Rh2CG176400

Calmodulin-binding receptor-like cytoplasmic kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000688
Physical Location & Seq
Forward (+)
16176454 .. 16177041
588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG176400.1

Sequence Viewer

Length: 588 bp
ATGGCACTGGACTTGATAGCAGGAGCAGCATTTGGGGCGTTGTATGACCGTGTTTCGCGAGCGGTGGACAAGAAGGTGGCGTTGAAAGACAGTCTCAAAGATATGAAACGCACATTGGAGTCCTTAAGAGGACAGGCTGGAATCATTCGAGGGATACGAGAGTTCAACGAGGAACTGAGTCTCCCAAACGATGGTATAGATGATCTCGAAACACAAATGATTGAAGGCGAAAAACTCATTTGCAGGTTGTCTTGTCTTGGTATCTACACCTACTGCTGCACAAGTGGCTGTGCTGAGCAGCTTGATGAATTGAATAGGTGTCTGGAAAGACTCTTGGAAACCTTGATGTTGCAGCAAGCAAGAGATGTGAAGGAGATCTTGAAAGGGTCAAGGCAAACCCGCGATGATGTTGATGAAATAAGGCAAATGATGAAGAAGATACTACTCCATATTGAAGGGGATTCGGTGGAGTCTCCAGCTTCAGAAACCACTACAGAGAATGAAGGAAATGGCGAGGAACAAGGTGAATTTCTTAGAAACTTGAAAATCAAAATTCTATACTATTGGAAATTGTTGTTGAAAGCCTAA

Protein Analysis

195

Amino Acids

22.11

Weight (kDa)

5.0

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 138 7.1e-14 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000688)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09820 FvH4_2g17160 FvH4_2g17160
malus_domestica MD02G1107100.v1.1 MD02G1107300.v1.1 MD05G1144900.v1.1 MD10G1144700.v1.1 MD15G1226500.v1.1
prunus_persica Prupe.7G188700_v2.0.a1 Prupe.7G188700_v2.0.a1 Prupe.7G188700_v2.0.a1 Prupe.8G183000_v2.0.a1
pyrus_communis pycom05g14000 pycom10g12440 pycom15g20140
rosa_chinensis RchiOBHm_Chr2g0096621 RchiOBHm_Chr6g0281601 RchiOBHm_Chr6g0281651 RchiOBHm_Chr7g0212121
rosa_laevigata RLG00000002926 RLG00000002927 RLG00000002933 RLG00000008638 RLG00000012954 RLG00000012955 RLG00000016656
rosa_multiflora Rmu_co8091766.1_g000001 Rmu_co8287847.1_g000001 Rmu_co8297221.1_g000001 Rmu_co8485327.1_g000001 Rmu_co8512767.1_g000001 Rmu_sc0002316.1_g000041 Rmu_sc0003565.1_g000009 Rmu_sc0014556.1_g000004 Rmu_sc0027573.1_g000001 Rmu_sc0030028.1_g000001
rosa_roxburghii Rroxscaffold_1G00026540 Rroxscaffold_2G00145470 Rroxscaffold_3G00247100 Rroxscaffold_3G00247110 Rroxscaffold_3G00247120 Rroxscaffold_5G00362680 Rroxscaffold_5G00362710 Rroxscaffold_7G00186950
rosa_rugosa Rorug02G0059000 Rorug02G0059100 Rorug06G0142300 Rorug07G0131000 Rorug07G0131100
rosa_samantha Rh1DG155700 Rh2AG106500 Rh2AG171500 Rh2BG108800 Rh2BG178900 Rh2CG110600 Rh2CG176400 Rh2DG110100 Rh2DG177600 Rh4BG120600 Rh6BG258300 Rh6CG257100 Rh6DG249200 Rh6DG249300 Rh7AG264100 Rh7AG264200 Rh7BG257800 Rh7BG257900 Rh7BG258000 Rh7BG258100 Rh7CG280600 Rh7CG280700 Rh7DG270600
rosa_wichuraiana Rw2G008240 Rw6G022110 Rw7G022540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 234
AccB7I CCANNNNNTGG 1 cut(s) 191
AccBSI CCGCTC 1 cut(s) 62
AccII CGCG 2 cut(s) 58, 402
AciI CCGC 2 cut(s) 62, 400
AcsI RAATTY 2 cut(s) 527, 552
AcuI CTGAAG 1 cut(s) 465
AfiI CCNNNNNNNGG 1 cut(s) 191
AflII CTTAAG 1 cut(s) 124
AgsI TTSAA 8 cut(s) 85, 166, 224, 313, 382, 455, 544, 580
AjuI GAANNNNNNNTTGG 2 cut(s) 98, 130
AloI GAACNNNNNNTCC 2 cut(s) 165, 197
AluBI AGCT 2 cut(s) 301, 479
AluI AGCT 2 cut(s) 301, 479
Alw26I GTCTC 3 cut(s) 98, 185, 477
ApeKI GCWGC 4 cut(s) 26, 276, 298, 352
ApoI RAATTY 2 cut(s) 527, 552
AsuHPI GGTGA 1 cut(s) 536
BaeI ACNNNNGTAYC 2 cut(s) 146, 179
BbvI GCAGC 4 cut(s) 38, 263, 310, 364
BccI CCATC 1 cut(s) 185
BciVI GTATCC 1 cut(s) 147
BcoDI GTCTC 3 cut(s) 98, 185, 477
BfmI CTRYAG 1 cut(s) 492
BfrI CTTAAG 1 cut(s) 124
BfuAI ACCTGC 1 cut(s) 234
BfuI GTATCC 1 cut(s) 147
BglII AGATCT 1 cut(s) 375
BisI GCNGC 4 cut(s) 27, 277, 299, 353
BlpI GCTNAGC 1 cut(s) 294
BlsI GCNGC 4 cut(s) 28, 278, 300, 354
BpmI CTGGAG 1 cut(s) 459
Bpu1102I GCTNAGC 1 cut(s) 294
BsaXI ACNNNNNCTCC 2 cut(s) 165, 195
Bsc4I CCNNNNNNNGG 1 cut(s) 191
Bse1I ACTGG 1 cut(s) 12
BseLI CCNNNNNNNGG 1 cut(s) 191
BseMII CTCAG 2 cut(s) 167, 285
BseNI ACTGG 1 cut(s) 12
BseXI GCAGC 4 cut(s) 38, 263, 310, 364
BsgI GTGCAG 1 cut(s) 262
Bsh1236I CGCG 2 cut(s) 58, 402
BslI CCNNNNNNNGG 1 cut(s) 191
BsmAI GTCTC 3 cut(s) 98, 185, 477
Bsp143I GATC 2 cut(s) 202, 375
Bsp1720I GCTNAGC 1 cut(s) 294
Bsp68I TCGCGA 1 cut(s) 58
BspACI CCGC 2 cut(s) 62, 400
BspCNI CTCAG 2 cut(s) 168, 286
BspFNI CGCG 2 cut(s) 58, 402
BspMI ACCTGC 1 cut(s) 234
BspTI CTTAAG 1 cut(s) 124
BsrBI CCGCTC 1 cut(s) 62
BsrI ACTGG 1 cut(s) 12
BssMI GATC 2 cut(s) 202, 375
Bst4CI ACNGT 2 cut(s) 50, 92
BstAFI CTTAAG 1 cut(s) 124
BstC8I GCNNGC 2 cut(s) 60, 357
BstDEI CTNAG 3 cut(s) 176, 294, 533
BstFNI CGCG 2 cut(s) 58, 402
BstKTI GATC 2 cut(s) 205, 378
BstMAI GTCTC 3 cut(s) 98, 185, 477
BstMBI GATC 2 cut(s) 202, 375
BstMWI GCNNNNNNNGC 3 cut(s) 26, 35, 285
BstSFI CTRYAG 1 cut(s) 492
BstUI CGCG 2 cut(s) 58, 402
BstV1I GCAGC 4 cut(s) 38, 263, 310, 364
BstX2I RGATCY 1 cut(s) 375
BstYI RGATCY 1 cut(s) 375
BsuI GTATCC 1 cut(s) 147
BtgZI GCGATG 1 cut(s) 417
BtsIMutI CAGTG 1 cut(s) 5
BtuMI TCGCGA 1 cut(s) 58
BveI ACCTGC 1 cut(s) 234
Cac8I GCNNGC 2 cut(s) 60, 357
CviJI RGCY 5 cut(s) 137, 288, 301, 479, 584
CviKI_1 RGCY 5 cut(s) 137, 288, 301, 479, 584
DdeI CTNAG 3 cut(s) 176, 294, 533
DpnI GATC 2 cut(s) 204, 377
DpnII GATC 2 cut(s) 202, 375
Eco57I CTGAAG 1 cut(s) 465
FaiI YATR 5 cut(s) 45, 104, 197, 450, 559
FalI AAGNNNNNCTT 2 cut(s) 362, 394
FauI CCCGC 1 cut(s) 407
Fnu4HI GCNGC 4 cut(s) 27, 277, 299, 353
Fsp4HI GCNGC 4 cut(s) 27, 277, 299, 353
GluI GCNGC 4 cut(s) 27, 277, 299, 353
GsuI CTGGAG 1 cut(s) 459
HinfI GANTC 6 cut(s) 119, 141, 178, 330, 461, 470
HphI GGTGA 1 cut(s) 536
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 1 cut(s) 484
Hpy188III TCNNGA 4 cut(s) 57, 206, 323, 379
Hpy8I GTNNAC 1 cut(s) 67
HpyAV CCTTC 5 cut(s) 67, 218, 364, 449, 497
HpyCH4III ACNGT 2 cut(s) 50, 92
HpyCH4V TGCA 3 cut(s) 243, 279, 352
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 35, 285
HpyF3I CTNAG 3 cut(s) 176, 294, 533
Kzo9I GATC 2 cut(s) 202, 375
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 6 cut(s) 6, 119, 123, 229, 308, 489
Lsp1109I GCAGC 4 cut(s) 38, 263, 310, 364
MalI GATC 2 cut(s) 204, 377
MbiI CCGCTC 1 cut(s) 62
MboI GATC 2 cut(s) 202, 375
MboII GAAGA 2 cut(s) 445, 448
MflI RGATCY 1 cut(s) 375
MluCI AATT 4 cut(s) 308, 527, 552, 569
MlyI GAGTC 4 cut(s) 128, 187, 324, 479
MnlI CCTC 4 cut(s) 122, 143, 163, 508
MseI TTAA 1 cut(s) 125
MspCI CTTAAG 1 cut(s) 124
MvnI CGCG 2 cut(s) 58, 402
MwoI GCNNNNNNNGC 3 cut(s) 26, 35, 285
NdeII GATC 2 cut(s) 202, 375
NruI TCGCGA 1 cut(s) 58
PcsI WCGNNNNNNNCGW 1 cut(s) 154
PfeI GAWTC 2 cut(s) 141, 461
PflMI CCANNNNNTGG 1 cut(s) 191
PkrI GCNGC 4 cut(s) 28, 278, 300, 354
PleI GAGTC 4 cut(s) 127, 186, 324, 478
PpsI GAGTC 4 cut(s) 127, 186, 324, 478
PsuI RGATCY 1 cut(s) 375
RruI TCGCGA 1 cut(s) 58
SaqAI TTAA 1 cut(s) 125
SatI GCNGC 4 cut(s) 27, 277, 299, 353
Sau3AI GATC 2 cut(s) 202, 375
SchI GAGTC 4 cut(s) 128, 187, 324, 479
SetI ASST 8 cut(s) 78, 248, 272, 303, 320, 344, 481, 526
SfcI CTRYAG 1 cut(s) 492
SmlI CTYRAG 1 cut(s) 124
SmoI CTYRAG 1 cut(s) 124
Sse9I AATT 4 cut(s) 308, 527, 552, 569
SsiI CCGC 2 cut(s) 62, 400
TaaI ACNGT 2 cut(s) 50, 92
TaqI TCGA 2 cut(s) 148, 207
TasI AATT 4 cut(s) 308, 527, 552, 569
TfiI GAWTC 2 cut(s) 141, 461
Tru1I TTAA 1 cut(s) 125
Tru9I TTAA 1 cut(s) 125
TscAI CASTG 1 cut(s) 12
TseI GCWGC 4 cut(s) 26, 276, 298, 352
TspDTI ATGAA 5 cut(s) 119, 321, 429, 446, 516
TspRI CASTG 1 cut(s) 12
Van91I CCANNNNNTGG 1 cut(s) 191
Vha464I CTTAAG 1 cut(s) 124
XapI RAATTY 2 cut(s) 527, 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.