RchiOBHm_Chr1g0340431

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000530
Physical Location & Seq
Reverse (-)
31988758 .. 31993199
4442 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56722

Sequence Viewer

Length: 792 bp
ATGGGTGGTTATGCTCGACGCAGTATGGTTGGTGCCAAGAGGGTGTTTGGTGAGATTTTGGACAGAGGGTGGTTTCCTGATGGGAAGCTGGTTGAGGCTATTAAGGTCATGGATGAGATGGAGGATAATGGAGTTGGCGCCAATGAGGTTACTTATGGGGTTATGATTAAAGCTTATTGTAAGGAAAAGAAGTCGGGCGAAGCGGTTAATTTGCTTGATGATATGGTAGAGAAGAGGTATATACCGAGCTCGGCGCTCTGTTGTAAGGTGATTGATGTTTTGTGTTGCGAAGGGAAGGTGGAGGATGCTTGTGTACTGTGGAAGAGGCTTTTGAAGAAGAATTGCACGCCGGATAATGCGGTATTGGGCACACTCATATACTGGCTTTGTAAGAAGGGAGAAGTGTGGGAAGCCAGGAGTTTGTTTGATCATGGGGAGGTTGTGGGGAGGTTGTGGGATGACATGGTGGAGAAAGGATATTCTCCGAATTCTTTTACATATAATGTATTGATCAAAGGATTCTGTAAGATTGGAAAAGCGAAGGAGGGAATTAGGATTTTGGACGAGATGTTCGAAAAAGGCTATTCGCCAAACAAGTCTACTTATGCTATGTTGATTGAGGGGCTCTGTGACTCGGTAGAGGATGCTGAAATGGATAGAGTAATATCTATGTCAATATCAAGTGGAGACATTAACAGTGATTCCTGGGATCTTTTCTTTACTAAAGTTGTTGGTGATTTGGATACTGGGGAAAGTGTTCTAGACAGAATACTACTGGAGAATGCTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

263

Amino Acids

29.4

Weight (kDa)

4.83

Isoelectric Point (pI)

29.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 27 - 61 9.3e-09 PPR repeat family
PPR_1 PF12854 44 - 76 7.2e-07 PPR repeat
PPR_2 PF13041 48 - 84 1.3e-07 PPR repeat family
PPR PF01535 50 - 79 3.8e-07 PPR repeat
PPR_2 PF13041 89 - 131 4.6e-06 PPR repeat family
PPR_2 PF13041 150 - 176 1.1e-09 PPR repeat family
PPR_3 PF13812 150 - 207 1.2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 158 - 190 5.7e-11 PPR repeat
PPR_2 PF13041 165 - 211 9.1e-15 PPR repeat family
PPR PF01535 165 - 194 1.2e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000530)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16420 AT5G16420 AT5G16420 AT5G16420
fragaria_vesca FvH4_7g08341 FvH4_7g08360
malus_domestica MD02G1232100.v1.1 MD07G1081300.v1.1
prunus_persica Prupe.2G106500_v2.0.a1
pyrus_communis pycom02g20040
rosa_chinensis RchiOBHm_Chr1g0340331 RchiOBHm_Chr1g0340431 RchiOBHm_Chr1g0340441 RchiOBHm_Chr1g0340491 RchiOBHm_Chr1g0340531 RchiOBHm_Chr1g0340541 RchiOBHm_Chr1g0340561 RchiOBHm_Chr1g0340621 RchiOBHm_Chr1g0340661 RchiOBHm_Chr1g0340691
rosa_laevigata RLG00000029013 RLG00000029018 RLG00000029020 RLG00000029024 RLG00000029026 RLG00000029029 RLG00000029030 RLG00000029032 RLG00000029033
rosa_multiflora Rmu_sc0000566.1_g000026 Rmu_sc0000566.1_g000034 Rmu_sc0000566.1_g000054 Rmu_sc0001836.1_g000016 Rmu_sc0001836.1_g000022 Rmu_sc0001836.1_g000023 Rmu_sc0001836.1_g000028 Rmu_sc0001836.1_g000058 Rmu_sc0001946.1_g000004 Rmu_sc0002595.1_g000001 Rmu_sc0002595.1_g000005 Rmu_sc0005167.1_g000003 Rmu_sc0005167.1_g000009 Rmu_sc0005167.1_g000018 Rmu_sc0024761.1_g000004
rosa_roxburghii Rroxscaffold_4G00311170 Rroxscaffold_4G00311190 Rroxscaffold_4G00311250 Rroxscaffold_4G00315930 Rroxscaffold_4G00315960 Rroxscaffold_5G00353270
rosa_rugosa Rorug01G0162000.1 Rorug01G0162500.1 Rorug01G0162800.1 Rorug01G0163000.1 Rorug01G0163400.1 Rorug01G0163700.1 Rorug01G0164100.1 Rorug01G0164300 Rorug05G0484800
rosa_samantha Rh1AG177800 Rh1AG178000 Rh1AG178300 Rh1AG178600 Rh1AG178900 Rh1BG145300 Rh1BG145600 Rh1BG145800 Rh1BG145900 Rh1BG146000 Rh1BG146700 Rh1CG165100 Rh1CG165200 Rh1CG165400 Rh1CG165600 Rh1CG165700 Rh1CG165900 Rh1DG177500 Rh1DG177600 Rh1DG177800 Rh1DG177900 Rh1DG178000 Rh1DG178800
rosa_wichuraiana Rw0G006210 Rw1G014800 Rw1G014890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 32, 137
AccI GTMKAC 1 cut(s) 599
AciI CCGC 2 cut(s) 203, 359
AclWI GGATC 1 cut(s) 717
AcsI RAATTY 1 cut(s) 487
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 1 cut(s) 315
AgsI TTSAA 1 cut(s) 334
AjnI CCWGG 2 cut(s) 413, 704
AloI GAACNNNNNNTCC 2 cut(s) 554, 586
AluBI AGCT 3 cut(s) 88, 173, 249
AluI AGCT 3 cut(s) 88, 173, 249
Alw21I GWGCWC 1 cut(s) 251
Alw26I GTCTC 1 cut(s) 681
AlwI GGATC 1 cut(s) 717
ApoI RAATTY 1 cut(s) 487
ArsI GACNNNNNNTTYG 2 cut(s) 554, 586
Asp700I GAANNNNTTC 1 cut(s) 756
AspLEI GCGC 2 cut(s) 140, 256
AsuHPI GGTGA 3 cut(s) 62, 280, 746
AsuII TTCGAA 1 cut(s) 573
BaeGI GKGCMC 1 cut(s) 371
BanI GGYRCC 2 cut(s) 32, 137
BanII GRGCYC 2 cut(s) 251, 627
Bbv12I GWGCWC 1 cut(s) 251
BccI CCATC 2 cut(s) 74, 112
BciT130I CCWGG 2 cut(s) 415, 706
BciVI GTATCC 1 cut(s) 736
BclI TGATCA 2 cut(s) 427, 510
BcoDI GTCTC 1 cut(s) 681
BfaI CTAG 1 cut(s) 761
BfoI RGCGCY 2 cut(s) 141, 257
BfuI GTATCC 1 cut(s) 736
Bme1390I CCNGG 2 cut(s) 415, 706
BmiI GGNNCC 2 cut(s) 34, 139
BmrFI CCNGG 2 cut(s) 415, 706
BmrI ACTGGG 1 cut(s) 756
BmsI GCATC 2 cut(s) 295, 634
BmuI ACTGGG 1 cut(s) 756
Bpu14I TTCGAA 1 cut(s) 573
BsaHI GRCGYC 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 705
Bse1I ACTGG 3 cut(s) 386, 751, 780
BseBI CCWGG 2 cut(s) 415, 706
BseDI CCNNGG 1 cut(s) 705
BseGI GGATG 4 cut(s) 118, 310, 463, 649
BseNI ACTGG 3 cut(s) 386, 751, 780
BseSI GKGCMC 1 cut(s) 371
BshNI GGYRCC 2 cut(s) 32, 137
BsiHKAI GWGCWC 1 cut(s) 251
BsiSI CCGG 1 cut(s) 350
BsmAI GTCTC 1 cut(s) 681
BsmI GAATGC 1 cut(s) 787
Bsp119I TTCGAA 1 cut(s) 573
Bsp1286I GDGCHC 3 cut(s) 251, 371, 627
Bsp143I GATC 3 cut(s) 427, 510, 709
BspACI CCGC 2 cut(s) 203, 359
BspLI GGNNCC 2 cut(s) 34, 139
BspPI GGATC 1 cut(s) 717
BspT104I TTCGAA 1 cut(s) 573
BspT107I GGYRCC 2 cut(s) 32, 137
BsrI ACTGG 3 cut(s) 386, 751, 780
BssECI CCNNGG 1 cut(s) 705
BssMI GATC 3 cut(s) 427, 510, 709
BssNI GRCGYC 1 cut(s) 138
Bst2UI CCWGG 2 cut(s) 415, 706
Bst4CI ACNGT 2 cut(s) 318, 698
Bst6I CTCTTC 2 cut(s) 227, 317
BstACI GRCGYC 1 cut(s) 138
BstBI TTCGAA 1 cut(s) 573
BstC8I GCNNGC 1 cut(s) 347
BstF5I GGATG 4 cut(s) 118, 310, 463, 649
BstH2I RGCGCY 2 cut(s) 141, 257
BstHHI GCGC 2 cut(s) 140, 256
BstKTI GATC 3 cut(s) 430, 513, 712
BstMAI GTCTC 1 cut(s) 681
BstMBI GATC 3 cut(s) 427, 510, 709
BstNI CCWGG 2 cut(s) 415, 706
BstSCI CCNGG 2 cut(s) 413, 704
BstSLI GKGCMC 1 cut(s) 371
BstX2I RGATCY 1 cut(s) 709
BstYI RGATCY 1 cut(s) 709
BsuI GTATCC 1 cut(s) 736
BtsCI GGATG 4 cut(s) 118, 310, 463, 649
BtsIMutI CAGTG 1 cut(s) 703
Cac8I GCNNGC 1 cut(s) 347
CfoI GCGC 2 cut(s) 140, 256
CseI GACGC 1 cut(s) 27
Csp6I GTAC 1 cut(s) 314
CviAII CATG 3 cut(s) 109, 431, 463
CviJI RGCY 9 cut(s) 88, 98, 173, 249, 328, 385, 413, 582, 625
CviKI_1 RGCY 9 cut(s) 88, 98, 173, 249, 328, 385, 413, 582, 625
CviQI GTAC 1 cut(s) 314
DinI GGCGCC 1 cut(s) 139
DpnI GATC 3 cut(s) 429, 512, 711
DpnII GATC 3 cut(s) 427, 510, 709
Eam1104I CTCTTC 2 cut(s) 227, 317
EarI CTCTTC 2 cut(s) 227, 317
Ecl136II GAGCTC 1 cut(s) 249
Eco24I GRGCYC 2 cut(s) 251, 627
Eco53kI GAGCTC 1 cut(s) 249
EcoICRI GAGCTC 1 cut(s) 249
EcoRI GAATTC 1 cut(s) 487
EcoRII CCWGG 2 cut(s) 413, 704
EcoT38I GRGCYC 2 cut(s) 251, 627
EgeI GGCGCC 1 cut(s) 139
EheI GGCGCC 1 cut(s) 139
FaeI CATG 3 cut(s) 112, 434, 466
FatI CATG 3 cut(s) 108, 430, 462
FbaI TGATCA 2 cut(s) 427, 510
FblI GTMKAC 1 cut(s) 599
FokI GGATG 4 cut(s) 125, 317, 470, 656
FriOI GRGCYC 2 cut(s) 251, 627
FspBI CTAG 1 cut(s) 761
GlaI GCGC 2 cut(s) 139, 255
HaeII RGCGCY 2 cut(s) 141, 257
HapII CCGG 1 cut(s) 350
HgaI GACGC 1 cut(s) 27
HhaI GCGC 2 cut(s) 140, 256
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 3 cut(s) 112, 434, 466
Hin6I GCGC 2 cut(s) 138, 254
HinP1I GCGC 2 cut(s) 138, 254
HindIII AAGCTT 1 cut(s) 171
HinfI GANTC 3 cut(s) 519, 632, 701
HpaII CCGG 1 cut(s) 350
HphI GGTGA 3 cut(s) 62, 280, 746
Hpy166II GTNNAC 2 cut(s) 314, 600
Hpy188I TCNGA 1 cut(s) 486
Hpy188III TCNNGA 2 cut(s) 77, 761
Hpy8I GTNNAC 2 cut(s) 314, 600
Hpy99I CGWCG 1 cut(s) 21
HpyAV CCTTC 4 cut(s) 284, 289, 388, 535
HpyCH4III ACNGT 2 cut(s) 318, 698
HpyCH4V TGCA 1 cut(s) 345
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 3 cut(s) 112, 434, 466
HspAI GCGC 2 cut(s) 138, 254
KasI GGCGCC 1 cut(s) 137
Ksp22I TGATCA 2 cut(s) 427, 510
Kzo9I GATC 3 cut(s) 427, 510, 709
LweI GCATC 2 cut(s) 295, 634
MaeI CTAG 1 cut(s) 761
MaeIII GTNAC 2 cut(s) 148, 629
MalI GATC 3 cut(s) 429, 512, 711
MboI GATC 3 cut(s) 427, 510, 709
MboII GAAGA 4 cut(s) 244, 334, 346, 349
MflI RGATCY 1 cut(s) 709
MhlI GDGCHC 3 cut(s) 251, 371, 627
MluCI AATT 4 cut(s) 208, 340, 487, 549
Mly113I GGCGCC 1 cut(s) 138
MlyI GAGTC 1 cut(s) 626
MroXI GAANNNNTTC 1 cut(s) 756
MseI TTAA 4 cut(s) 102, 168, 207, 693
MspI CCGG 1 cut(s) 350
MspR9I CCNGG 2 cut(s) 415, 706
Mva1269I GAATGC 1 cut(s) 787
MvaI CCWGG 2 cut(s) 415, 706
NarI GGCGCC 1 cut(s) 138
NdeII GATC 3 cut(s) 427, 510, 709
NlaIII CATG 3 cut(s) 112, 434, 466
NlaIV GGNNCC 2 cut(s) 34, 139
NmeAIII GCCGAG 1 cut(s) 230
NmuCI GTSAC 1 cut(s) 629
NspV TTCGAA 1 cut(s) 573
PcsI WCGNNNNNNNCGW 1 cut(s) 570
PctI GAATGC 1 cut(s) 787
PdmI GAANNNNTTC 1 cut(s) 756
PfeI GAWTC 2 cut(s) 519, 701
PleI GAGTC 1 cut(s) 626
PluTI GGCGCC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 626
Psp124BI GAGCTC 1 cut(s) 251
Psp6I CCWGG 2 cut(s) 413, 704
PspGI CCWGG 2 cut(s) 413, 704
PspN4I GGNNCC 2 cut(s) 34, 139
PsuI RGATCY 1 cut(s) 709
RsaI GTAC 1 cut(s) 315
RsaNI GTAC 1 cut(s) 314
SacI GAGCTC 1 cut(s) 251
SaqAI TTAA 4 cut(s) 102, 168, 207, 693
Sau3AI GATC 3 cut(s) 427, 510, 709
SchI GAGTC 1 cut(s) 626
ScrFI CCNGG 2 cut(s) 415, 706
SduI GDGCHC 3 cut(s) 251, 371, 627
SfaNI GCATC 2 cut(s) 295, 634
SfoI GGCGCC 1 cut(s) 139
SfuI TTCGAA 1 cut(s) 573
Sse9I AATT 4 cut(s) 208, 340, 487, 549
SsiI CCGC 2 cut(s) 203, 359
SspDI GGCGCC 1 cut(s) 137
SspMI CTAG 1 cut(s) 761
SstI GAGCTC 1 cut(s) 251
StyD4I CCNGG 2 cut(s) 413, 704
TaaI ACNGT 2 cut(s) 318, 698
TaqI TCGA 2 cut(s) 16, 573
TasI AATT 4 cut(s) 208, 340, 487, 549
TatI WGTACW 1 cut(s) 313
TfiI GAWTC 2 cut(s) 519, 701
Tru1I TTAA 4 cut(s) 102, 168, 207, 693
Tru9I TTAA 4 cut(s) 102, 168, 207, 693
TscAI CASTG 1 cut(s) 703
TseFI GTSAC 1 cut(s) 629
Tsp45I GTSAC 1 cut(s) 629
TspRI CASTG 1 cut(s) 703
XapI RAATTY 1 cut(s) 487
XbaI TCTAGA 1 cut(s) 760
XmiI GTMKAC 1 cut(s) 599
XmnI GAANNNNTTC 1 cut(s) 756
XspI CTAG 1 cut(s) 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.