Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0000530

Physical Location & Seq

Genomic Coordinates Reverse (-)

31604959 .. 31610684

Gene Structure 5726 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000029020

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2112 bp

ATGGAGGCCATCAAGTTGTTTGGGCATCTCAACTCACTTGAAACCTTGAAGATTGCTCATGGATACTGGGCAGCCCACACCTCTCTTTCTTCTGTACGGCTTGTGCATAGCAGCGGTTTGCAGGCCTACATTTTGAGGGACAAGAAGCTTCTTAATGAACTAGTTCTGAATTGGGCAGAATGGGATGTAAATGAAGAAAAAGCAGAGTCTGATAATTTGAATAATTTAGTAGATTTGAATTGGGAAGGAATCATAAGGGAAGAAATAGAAGAGCTCAATGAACAAGTTTTGGATTGGGGAGGAAACAGAACTGAAGAAAGAGAAGTGCTTGAGAAGCTCCAACCTCACCTGAACCTGGAAAGGCTTAGAATTAGAGAATATTATTATTCCTCTGCTCTGGTTGATCTTCAACTTGTTAATTGTGGGAATTGCATCTCCTTGCCACCACTGGGACAGCTACCTTCCCTCAGCGAGCTTTATATTTCTGGGTTACATGGAGTGGTGTCTATAGTTCACCATGCTGCTTCGCCGGAACCTCTCCCCCAAACCCTTCTGTCTCCTCCACTCAATCACCGCCGTTCCTTCTCCACCTCCACCTCCGCCGTCGATTTCCTCCCCTCCTACACCGTCACCCCTCCAATCCAAGACTGGCCCCACCACCTCCACCCAAAGCACCTCATCTCCCTCATCACCCGCGAGCCCAAACTCGACCTCGCCCTCCAAATCTTCCACCACGCCTCCAAATACCACCCCGGATTCCACCATAATTACCACACCTACCACGCCATCATCCGCCGCCTCCTCCGCTCCCGCGCTTTTCACCACATCGATCCCCTCCTCTCCGACCTCCGCACCTCCAATCTCCGATGCAGCGAAGACCTCTTCATCTCCATAATCCGCAACTACGACGTCGCTTCACGTCCCAAACTCGCTTTCAAGACATTTATCACCATTCCGAAATTCGGCGTACAACGATCCATGAGGTCCTTGAATGCGCTGCTAAACGCTTTGGTTCAGAATCACGAGTATAGATTGGTGTTTCGGAACTGCAGGGAGAGGTTTGAGGTGGCGCCGAATGTGTTTAGTTGTAATATATTGATCAAAGCACTATGTAAGAGAAATGATTTGGAGACTGCACACCAGGTGTTCGACGAAATGCCTGCGATAGGATTTGTGCCCAATGTGGTGACTTATACTACCATTTTGGGAGGTTATGTGTCGCGGCGCGATATGGTTGGTGCCAAGAGGGTGTTTGGTGAGATTTTGGACAGAGGGTGGTTTCCTGATGTGACTACATATACTATATTGATGGATGGGTATGTGAAGCAGGGGAAGCTGGTTGAGGCTATTAAGGTTATGGATGAGATGGAGGATAATGGGGTTGGCGCCAATGAGGTTACTTATGGGGTTATGATTGAAGCTTATTGTAAGGAAAAGAAGTCGGGCGAAGCGGTTAATTTGCTTAATGATATGGTGGAGAAGAGGTATATACCAAGCTCGGCGCTCTGTTGTAAGGTGATTGATGTTTTGTGTTGCGAAGGGAAGGTGGAGGATGCTTGTGAACTGTGGAAGAGGCTTTTGAAGAAGAATTGCACGCCGGATAATGCGGTATTGGGCACGCTCATATACTGGCTTTGTAAGAAGGGAGAAGTGTGGGAAGCCAGGAGTTTGTTTGATCAGTTTGAGATGAGTGAGCCTCCAGATGTCATGACTTACAACGTGCTTATTTCTGGAATGTGTGAAGTGGGGGAGTTGTGTGAGGCAGGGAGGTTGTGGGATGACATGGTGGAGAAAGGTTATTCTCCGAATTCTTTTACATATAATGTATTGATCAAAGGATTCTGTAAGATTGGAAAAGCGAAGGAGGGAATTAGGATTTTGGACGAGATGTTCGAAAAAGGCTGTTTGCCAAACAAGTCTACTTATGCTATGTTGATTGAGGGGCTCTGCGACTCAGGAGAGGAAGCTGAAATCACTAGAGTTATATCTATGGCAATGTCAAGTGGAGACATTGACAGTGATTCCTGGGATCTTTTCCTTACTAAGGTTGTTCGTGATTTGGATTCTGGGGAAAGTGTTCTAAACAGAATATTACTGGAGAATGCTGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

704

Amino Acids

79.93

Weight (kDa)

5.68

Isoelectric Point (pI)

40.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_R13L1-DRL21	PF25019	91 - 163	7.5e-12	R13L1/DRL21 LRRs
PPR_3	PF13812	347 - 407	5.1e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	356 - 386	8.4e-08	PPR repeat
PPR_2	PF13041	358 - 405	6.2e-15	PPR repeat family
PPR_2	PF13041	393 - 442	1.6e-13	PPR repeat family
PPR_3	PF13812	417 - 477	8.8e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	425 - 457	7.3e-09	PPR repeat
PPR_2	PF13041	428 - 477	3.4e-16	PPR repeat family
PPR	PF01535	432 - 461	4.1e-08	PPR repeat
PPR_1	PF12854	460 - 492	6.5e-06	PPR repeat
PPR_2	PF13041	464 - 500	3.3e-07	PPR repeat family
PPR	PF01535	466 - 495	3.9e-06	PPR repeat
PPR_2	PF13041	505 - 547	3.5e-06	PPR repeat family
PPR_2	PF13041	567 - 616	4e-19	PPR repeat family
PPR_1	PF12854	567 - 596	1.2e-07	PPR repeat
PPR_3	PF13812	567 - 614	1.2e-06	Pentatricopeptide repeat domain
PPR_3	PF13812	590 - 647	9.8e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	598 - 630	2e-10	PPR repeat
PPR_2	PF13041	605 - 651	1.9e-14	PPR repeat family
PPR	PF01535	605 - 635	1.1e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000530)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16420 AT5G16420 AT5G16420 AT5G16420
fragaria_vesca	FvH4_7g08341 FvH4_7g08360
malus_domestica	MD02G1232100.v1.1 MD07G1081300.v1.1
prunus_persica	Prupe.2G106500_v2.0.a1
pyrus_communis	pycom02g20040
rosa_chinensis	RchiOBHm_Chr1g0340331 RchiOBHm_Chr1g0340431 RchiOBHm_Chr1g0340441 RchiOBHm_Chr1g0340491 RchiOBHm_Chr1g0340531 RchiOBHm_Chr1g0340541 RchiOBHm_Chr1g0340561 RchiOBHm_Chr1g0340621 RchiOBHm_Chr1g0340661 RchiOBHm_Chr1g0340691
rosa_laevigata	RLG00000029013 RLG00000029018 RLG00000029020 RLG00000029024 RLG00000029026 RLG00000029029 RLG00000029030 RLG00000029032 RLG00000029033
rosa_multiflora	Rmu_sc0000566.1_g000026 Rmu_sc0000566.1_g000034 Rmu_sc0000566.1_g000054 Rmu_sc0001836.1_g000016 Rmu_sc0001836.1_g000022 Rmu_sc0001836.1_g000023 Rmu_sc0001836.1_g000028 Rmu_sc0001836.1_g000058 Rmu_sc0001946.1_g000004 Rmu_sc0002595.1_g000001 Rmu_sc0002595.1_g000005 Rmu_sc0005167.1_g000003 Rmu_sc0005167.1_g000009 Rmu_sc0005167.1_g000018 Rmu_sc0024761.1_g000004
rosa_roxburghii	Rroxscaffold_4G00311170 Rroxscaffold_4G00311190 Rroxscaffold_4G00311250 Rroxscaffold_4G00315930 Rroxscaffold_4G00315960 Rroxscaffold_5G00353270
rosa_rugosa	Rorug01G0162000.1 Rorug01G0162500.1 Rorug01G0162800.1 Rorug01G0163000.1 Rorug01G0163400.1 Rorug01G0163700.1 Rorug01G0164100.1 Rorug01G0164300 Rorug05G0484800
rosa_samantha	Rh1AG177800 Rh1AG178000 Rh1AG178300 Rh1AG178600 Rh1AG178900 Rh1BG145300 Rh1BG145600 Rh1BG145800 Rh1BG145900 Rh1BG146000 Rh1BG146700 Rh1CG165100 Rh1CG165200 Rh1CG165400 Rh1CG165600 Rh1CG165700 Rh1CG165900 Rh1DG177500 Rh1DG177600 Rh1DG177800 Rh1DG177900 Rh1DG178000 Rh1DG178800
rosa_wichuraiana	Rw0G006210 Rw1G014800 Rw1G014890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	912
AccB1I	GGYRCC	3 cut(s)	1069, 1238, 1385
AccBSI	CCGCTC	1 cut(s)	807
AccI	GTMKAC	1 cut(s)	1919
AccII	CGCG	4 cut(s)	696, 813, 1222, 1227
AclWI	GGATC	3 cut(s)	824, 969, 2037
AcsI	RAATTY	2 cut(s)	959, 1807
AcuI	CTGAAG	1 cut(s)	333
AcyI	GRCGYC	3 cut(s)	909, 1070, 1386
AdeI	CACNNNGTG	1 cut(s)	1144
AfaI	GTAC	2 cut(s)	96, 969
AfiI	CCNNNNNNNGG	5 cut(s)	355, 449, 962, 1166, 2044
AgsI	TTSAA	9 cut(s)	41, 49, 220, 238, 410, 937, 991, 1418, 1582
AhlI	ACTAGT	1 cut(s)	160
AjiI	CACGTC	1 cut(s)	920
AjnI	CCWGG	4 cut(s)	354, 1140, 1661, 2024
AjuI	GAANNNNNNNTTGG	2 cut(s)	917, 949
AloI	GAACNNNNNNTCC	2 cut(s)	1874, 1906
AluBI	AGCT	9 cut(s)	148, 274, 337, 457, 475, 1336, 1421, 1497, 1967
AluI	AGCT	9 cut(s)	148, 274, 337, 457, 475, 1336, 1421, 1497, 1967
Alw21I	GWGCWC	1 cut(s)	276
Alw26I	GTCTC	3 cut(s)	561, 1124, 2001
AlwI	GGATC	3 cut(s)	824, 969, 2037
AlwNI	CAGNNNCTG	1 cut(s)	209
AoxI	GGCC	3 cut(s)	6, 123, 650
ApeKI	GCWGC	5 cut(s)	71, 111, 521, 870, 997
ApoI	RAATTY	2 cut(s)	959, 1807
ArsI	GACNNNNNNTTYG	2 cut(s)	1874, 1906
Asp700I	GAANNNNTTC	2 cut(s)	162, 2076
AspLEI	GCGC	6 cut(s)	815, 997, 1072, 1227, 1388, 1504
AspS9I	GGNCC	2 cut(s)	651, 984
AsuC2I	CCSGG	1 cut(s)	753
AsuII	TTCGAA	1 cut(s)	1893
AvaII	GGWCC	1 cut(s)	984
BaeGI	GKGCMC	2 cut(s)	1179, 1619
BanI	GGYRCC	3 cut(s)	1069, 1238, 1385
BanII	GRGCYC	3 cut(s)	276, 702, 1947
BauI	CACGAG	1 cut(s)	1022
BbsI	GAAGAC	1 cut(s)	882
Bbv12I	GWGCWC	1 cut(s)	276
BbvCI	CCTCAGC	1 cut(s)	467
BbvI	GCAGC	5 cut(s)	83, 123, 508, 882, 984
BccI	CCATC	5 cut(s)	17, 794, 1303, 1307, 1360
BceAI	ACGGC	3 cut(s)	113, 561, 587
BcgI	CGANNNNNNTGC	2 cut(s)	1142, 1176
BciT130I	CCWGG	4 cut(s)	356, 1142, 1663, 2026
BciVI	GTATCC	1 cut(s)	56
BclI	TGATCA	3 cut(s)	1098, 1675, 1830
BcnI	CCSGG	1 cut(s)	753
BcoDI	GTCTC	3 cut(s)	561, 1124, 2001
BcuI	ACTAGT	1 cut(s)	160
BfaI	CTAG	2 cut(s)	161, 1977
BfmI	CTRYAG	2 cut(s)	507, 1048
BfoI	RGCGCY	3 cut(s)	1073, 1389, 1505
BfuI	GTATCC	1 cut(s)	56
BisI	GCNGC	7 cut(s)	72, 112, 522, 796, 871, 998, 1223
BlsI	GCNGC	7 cut(s)	73, 113, 523, 797, 872, 999, 1224
Bme1390I	CCNGG	5 cut(s)	356, 753, 1142, 1663, 2026
Bme18I	GGWCC	1 cut(s)	984
BmgBI	CACGTC	1 cut(s)	920
BmgT120I	GGNCC	2 cut(s)	651, 984
BmiI	GGNNCC	5 cut(s)	534, 653, 1071, 1240, 1387
BmrFI	CCNGG	5 cut(s)	356, 753, 1142, 1663, 2026
BmrI	ACTGGG	2 cut(s)	76, 458
BmsI	GCATC	4 cut(s)	34, 441, 857, 1543
BmuI	ACTGGG	2 cut(s)	76, 458
BpiI	GAAGAC	1 cut(s)	882
BplI	GAGNNNNNCTC	2 cut(s)	1681, 1713
BpmI	CTGGAG	1 cut(s)	1683
Bpu10I	CCTNAGC	1 cut(s)	467
Bpu14I	TTCGAA	1 cut(s)	1893
BpuEI	CTTGAG	1 cut(s)	350
BpuMI	CCSGG	1 cut(s)	753
Bsa29I	ATCGAT	1 cut(s)	828
BsaHI	GRCGYC	3 cut(s)	909, 1070, 1386
BsaJI	CCNNGG	2 cut(s)	751, 2025
BsaXI	ACNNNNNCTCC	8 cut(s)	665, 695, 722, 752, 1200, 1230, 1469, 1499
Bsc4I	CCNNNNNNNGG	5 cut(s)	355, 449, 962, 1166, 2044
Bse1I	ACTGG	5 cut(s)	71, 453, 653, 1634, 2100
Bse3DI	GCAATG	1 cut(s)	2001
BseBI	CCWGG	4 cut(s)	356, 1142, 1663, 2026
BseCI	ATCGAT	1 cut(s)	828
BseDI	CCNNGG	2 cut(s)	751, 2025
BseGI	GGATG	6 cut(s)	190, 789, 1318, 1366, 1558, 1783
BseLI	CCNNNNNNNGG	5 cut(s)	355, 449, 962, 1166, 2044
BseMI	GCAATG	1 cut(s)	2001
BseMII	CTCAG	2 cut(s)	481, 1968
BseNI	ACTGG	5 cut(s)	71, 453, 653, 1634, 2100
BseRI	GAGGAG	3 cut(s)	549, 791, 827
BseSI	GKGCMC	2 cut(s)	1179, 1619
BseXI	GCAGC	5 cut(s)	83, 123, 508, 882, 984
BsgI	GTGCAG	1 cut(s)	1119
Bsh1236I	CGCG	4 cut(s)	696, 813, 1222, 1227
BshFI	GGCC	3 cut(s)	8, 125, 652
BshNI	GGYRCC	3 cut(s)	1069, 1238, 1385
BshVI	ATCGAT	1 cut(s)	828
BsiHKAI	GWGCWC	1 cut(s)	276
BsiSI	CCGG	3 cut(s)	530, 753, 1598
BslFI	GGGAC	3 cut(s)	152, 465, 906
BslI	CCNNNNNNNGG	5 cut(s)	355, 449, 962, 1166, 2044
BsmAI	GTCTC	3 cut(s)	561, 1124, 2001
BsmFI	GGGAC	3 cut(s)	152, 465, 906
BsmI	GAATGC	2 cut(s)	997, 2107
BsnI	GGCC	3 cut(s)	8, 125, 652
Bsp119I	TTCGAA	1 cut(s)	1893
Bsp1286I	GDGCHC	5 cut(s)	276, 702, 1179, 1619, 1947
Bsp143I	GATC	7 cut(s)	403, 829, 974, 1098, 1675, 1830, 2029
BspANI	GGCC	3 cut(s)	8, 125, 652
BspCNI	CTCAG	2 cut(s)	480, 1967
BspDI	ATCGAT	1 cut(s)	828
BspFNI	CGCG	4 cut(s)	696, 813, 1222, 1227
BspHI	TCATGA	1 cut(s)	1707
BspLI	GGNNCC	5 cut(s)	534, 653, 1071, 1240, 1387
BspMAI	CTGCAG	1 cut(s)	1052
BspPI	GGATC	3 cut(s)	824, 969, 2037
BspQI	GCTCTTC	1 cut(s)	264
BspT104I	TTCGAA	1 cut(s)	1893
BspT107I	GGYRCC	3 cut(s)	1069, 1238, 1385
BsrBI	CCGCTC	1 cut(s)	807
BsrDI	GCAATG	1 cut(s)	2001
BsrI	ACTGG	5 cut(s)	71, 453, 653, 1634, 2100
BssECI	CCNNGG	2 cut(s)	751, 2025
BssMI	GATC	7 cut(s)	403, 829, 974, 1098, 1675, 1830, 2029
BssNI	GRCGYC	3 cut(s)	909, 1070, 1386
BssSI	CACGAG	1 cut(s)	1022
Bst2BI	CACGAG	1 cut(s)	1022
Bst2UI	CCWGG	4 cut(s)	356, 1142, 1663, 2026
Bst4CI	ACNGT	3 cut(s)	628, 1566, 2018
Bst6I	CTCTTC	4 cut(s)	264, 887, 1475, 1565
BstACI	GRCGYC	3 cut(s)	909, 1070, 1386
BstBI	TTCGAA	1 cut(s)	1893
BstC8I	GCNNGC	6 cut(s)	123, 473, 698, 1161, 1595, 1619
BstDEI	CTNAG	4 cut(s)	365, 467, 1954, 2043
BstENI	CCTNNNNNAGG	2 cut(s)	1164, 2042
BstF5I	GGATG	6 cut(s)	190, 789, 1318, 1366, 1558, 1783
BstFNI	CGCG	4 cut(s)	696, 813, 1222, 1227
BstH2I	RGCGCY	3 cut(s)	1073, 1389, 1505
BstHHI	GCGC	6 cut(s)	815, 997, 1072, 1227, 1388, 1504
BstKTI	GATC	7 cut(s)	406, 832, 977, 1101, 1678, 1833, 2032
BstMAI	GTCTC	3 cut(s)	561, 1124, 2001
BstMBI	GATC	7 cut(s)	403, 829, 974, 1098, 1675, 1830, 2029
BstMWI	GCNNNNNNNGC	3 cut(s)	334, 804, 1333
BstNI	CCWGG	4 cut(s)	356, 1142, 1663, 2026
BstSCI	CCNGG	5 cut(s)	354, 751, 1140, 1661, 2024
BstSFI	CTRYAG	2 cut(s)	507, 1048
BstSLI	GKGCMC	2 cut(s)	1179, 1619
BstUI	CGCG	4 cut(s)	696, 813, 1222, 1227
BstV1I	GCAGC	5 cut(s)	83, 123, 508, 882, 984
BstV2I	GAAGAC	1 cut(s)	882
BstX2I	RGATCY	1 cut(s)	2029
BstYI	RGATCY	1 cut(s)	2029
Bsu15I	ATCGAT	1 cut(s)	828
BsuI	GTATCC	1 cut(s)	56
BsuRI	GGCC	3 cut(s)	8, 125, 652
BsuTUI	ATCGAT	1 cut(s)	828
BtrI	CACGTC	1 cut(s)	920
BtsCI	GGATG	6 cut(s)	190, 789, 1318, 1366, 1558, 1783
BtsIMutI	CAGTG	2 cut(s)	446, 2023
Cac8I	GCNNGC	6 cut(s)	123, 473, 698, 1161, 1595, 1619
CaiI	CAGNNNCTG	1 cut(s)	209
CciI	TCATGA	1 cut(s)	1707
CfoI	GCGC	6 cut(s)	815, 997, 1072, 1227, 1388, 1504
Cfr13I	GGNCC	2 cut(s)	651, 984
ClaI	ATCGAT	1 cut(s)	828
CsiI	ACCWGGT	1 cut(s)	1140
Csp6I	GTAC	2 cut(s)	95, 968
CviAII	CATG	6 cut(s)	59, 494, 518, 979, 1708, 1783
CviQI	GTAC	2 cut(s)	95, 968
DdeI	CTNAG	4 cut(s)	365, 467, 1954, 2043
DinI	GGCGCC	2 cut(s)	1071, 1387
DpnI	GATC	7 cut(s)	405, 831, 976, 1100, 1677, 1832, 2031
DpnII	GATC	7 cut(s)	403, 829, 974, 1098, 1675, 1830, 2029
DraIII	CACNNNGTG	1 cut(s)	1144
Eam1104I	CTCTTC	4 cut(s)	264, 887, 1475, 1565
EarI	CTCTTC	4 cut(s)	264, 887, 1475, 1565
EciI	GGCGGA	2 cut(s)	589, 782
Ecl136II	GAGCTC	1 cut(s)	274
Eco147I	AGGCCT	1 cut(s)	125
Eco24I	GRGCYC	3 cut(s)	276, 702, 1947
Eco47I	GGWCC	1 cut(s)	984
Eco53kI	GAGCTC	1 cut(s)	274
Eco57I	CTGAAG	1 cut(s)	333
EcoICRI	GAGCTC	1 cut(s)	274
EcoNI	CCTNNNNNAGG	2 cut(s)	1164, 2042
EcoO109I	RGGNCCY	1 cut(s)	984
EcoRI	GAATTC	1 cut(s)	1807
EcoRII	CCWGG	4 cut(s)	354, 1140, 1661, 2024
EcoT38I	GRGCYC	3 cut(s)	276, 702, 1947
EgeI	GGCGCC	2 cut(s)	1071, 1387
EheI	GGCGCC	2 cut(s)	1071, 1387
FaeI	CATG	6 cut(s)	62, 497, 521, 982, 1711, 1786
FaqI	GGGAC	3 cut(s)	152, 465, 906
FatI	CATG	6 cut(s)	58, 493, 517, 978, 1707, 1782
FauI	CCCGC	2 cut(s)	701, 818
FbaI	TGATCA	3 cut(s)	1098, 1675, 1830
FblI	GTMKAC	1 cut(s)	1919
Fnu4HI	GCNGC	7 cut(s)	72, 112, 522, 796, 871, 998, 1223
FokI	GGATG	6 cut(s)	197, 776, 1325, 1373, 1565, 1790
FriOI	GRGCYC	3 cut(s)	276, 702, 1947
Fsp4HI	GCNGC	7 cut(s)	72, 112, 522, 796, 871, 998, 1223
FspBI	CTAG	2 cut(s)	161, 1977
GlaI	GCGC	6 cut(s)	814, 996, 1071, 1226, 1387, 1503
GluI	GCNGC	7 cut(s)	72, 112, 522, 796, 871, 998, 1223
GsuI	CTGGAG	1 cut(s)	1683
HaeII	RGCGCY	3 cut(s)	1073, 1389, 1505
HaeIII	GGCC	3 cut(s)	8, 125, 652
HapII	CCGG	3 cut(s)	530, 753, 1598
HhaI	GCGC	6 cut(s)	815, 997, 1072, 1227, 1388, 1504
Hin1I	GRCGYC	3 cut(s)	909, 1070, 1386
Hin1II	CATG	6 cut(s)	62, 497, 521, 982, 1711, 1786
Hin6I	GCGC	6 cut(s)	813, 995, 1070, 1225, 1386, 1502
HinP1I	GCGC	6 cut(s)	813, 995, 1070, 1225, 1386, 1502
HindIII	AAGCTT	2 cut(s)	146, 1419
HinfI	GANTC	8 cut(s)	206, 249, 756, 1018, 1839, 1952, 2021, 2063
HpaII	CCGG	3 cut(s)	530, 753, 1598
Hpy166II	GTNNAC	3 cut(s)	514, 1562, 1920
Hpy188I	TCNGA	8 cut(s)	168, 211, 844, 866, 957, 1017, 1044, 1806
Hpy188III	TCNNGA	8 cut(s)	937, 1022, 1283, 1700, 1708, 1731, 1956, 2054
Hpy8I	GTNNAC	3 cut(s)	514, 1562, 1920
Hpy99I	CGWCG	4 cut(s)	608, 911, 914, 1154
HpyAV	CCTTC	8 cut(s)	239, 471, 560, 592, 1532, 1537, 1636, 1855
HpyCH4III	ACNGT	3 cut(s)	628, 1566, 2018
HpyCH4IV	ACGT	3 cut(s)	909, 919, 1719
HpyCH4V	TGCA	7 cut(s)	106, 121, 432, 870, 1050, 1136, 1593
HpyF10VI	GCNNNNNNNGC	3 cut(s)	334, 804, 1333
HpyF3I	CTNAG	4 cut(s)	365, 467, 1954, 2043
HpySE526I	ACGT	3 cut(s)	909, 919, 1719
Hsp92I	GRCGYC	3 cut(s)	909, 1070, 1386
Hsp92II	CATG	6 cut(s)	62, 497, 521, 982, 1711, 1786
HspAI	GCGC	6 cut(s)	813, 995, 1070, 1225, 1386, 1502
KasI	GGCGCC	2 cut(s)	1069, 1385
Ksp22I	TGATCA	3 cut(s)	1098, 1675, 1830
Kzo9I	GATC	7 cut(s)	403, 829, 974, 1098, 1675, 1830, 2029
LguI	GCTCTTC	1 cut(s)	264
LmnI	GCTCC	2 cut(s)	342, 812
Lsp1109I	GCAGC	5 cut(s)	83, 123, 508, 882, 984
LweI	GCATC	4 cut(s)	34, 441, 857, 1543
MabI	ACCWGGT	1 cut(s)	1140
MaeI	CTAG	2 cut(s)	161, 1977
MaeII	ACGT	3 cut(s)	909, 919, 1719
MaeIII	GTNAC	5 cut(s)	489, 628, 1186, 1288, 1396
MalI	GATC	7 cut(s)	405, 831, 976, 1100, 1677, 1832, 2031
MbiI	CCGCTC	1 cut(s)	807
MboI	GATC	7 cut(s)	403, 829, 974, 1098, 1675, 1830, 2029
MflI	RGATCY	1 cut(s)	2029
MhlI	GDGCHC	5 cut(s)	276, 702, 1179, 1619, 1947
Mly113I	GGCGCC	2 cut(s)	1070, 1386
MlyI	GAGTC	2 cut(s)	215, 1946
MmeI	TCCRAC	2 cut(s)	364, 867
MroXI	GAANNNNTTC	2 cut(s)	162, 2076
MseI	TTAA	5 cut(s)	153, 417, 1350, 1455, 1464
MspA1I	CMGCKG	1 cut(s)	114
MspI	CCGG	3 cut(s)	530, 753, 1598
MspR9I	CCNGG	5 cut(s)	356, 753, 1142, 1663, 2026
Mva1269I	GAATGC	2 cut(s)	997, 2107
MvaI	CCWGG	4 cut(s)	356, 1142, 1663, 2026
MvnI	CGCG	4 cut(s)	696, 813, 1222, 1227
MwoI	GCNNNNNNNGC	3 cut(s)	334, 804, 1333
NarI	GGCGCC	2 cut(s)	1070, 1386
NciI	CCSGG	1 cut(s)	753
NdeII	GATC	7 cut(s)	403, 829, 974, 1098, 1675, 1830, 2029
NlaIII	CATG	6 cut(s)	62, 497, 521, 982, 1711, 1786
NlaIV	GGNNCC	5 cut(s)	534, 653, 1071, 1240, 1387
NmeAIII	GCCGAG	1 cut(s)	1478
NmuCI	GTSAC	3 cut(s)	628, 1186, 1288
NspV	TTCGAA	1 cut(s)	1893
PagI	TCATGA	1 cut(s)	1707
PceI	AGGCCT	1 cut(s)	125
PciSI	GCTCTTC	1 cut(s)	264
PcsI	WCGNNNNNNNCGW	1 cut(s)	1890
PctI	GAATGC	2 cut(s)	997, 2107
PdmI	GAANNNNTTC	2 cut(s)	162, 2076
PfeI	GAWTC	6 cut(s)	249, 756, 1018, 1839, 2021, 2063
PkrI	GCNGC	7 cut(s)	73, 113, 523, 797, 872, 999, 1224
PleI	GAGTC	2 cut(s)	214, 1946
PluTI	GGCGCC	2 cut(s)	1073, 1389
PpsI	GAGTC	2 cut(s)	214, 1946
PpuMI	RGGWCCY	1 cut(s)	984
Psp124BI	GAGCTC	1 cut(s)	276
Psp5II	RGGWCCY	1 cut(s)	984
Psp6I	CCWGG	4 cut(s)	354, 1140, 1661, 2024
PspGI	CCWGG	4 cut(s)	354, 1140, 1661, 2024
PspN4I	GGNNCC	5 cut(s)	534, 653, 1071, 1240, 1387
PspPI	GGNCC	2 cut(s)	651, 984
PspPPI	RGGWCCY	1 cut(s)	984
PstI	CTGCAG	1 cut(s)	1052
PstNI	CAGNNNCTG	1 cut(s)	209
PsuI	RGATCY	1 cut(s)	2029
RsaI	GTAC	2 cut(s)	96, 969
RsaNI	GTAC	2 cut(s)	95, 968
SacI	GAGCTC	1 cut(s)	276
SapI	GCTCTTC	1 cut(s)	264
SaqAI	TTAA	5 cut(s)	153, 417, 1350, 1455, 1464
SatI	GCNGC	7 cut(s)	72, 112, 522, 796, 871, 998, 1223
Sau3AI	GATC	7 cut(s)	403, 829, 974, 1098, 1675, 1830, 2029
Sau96I	GGNCC	2 cut(s)	651, 984
SchI	GAGTC	2 cut(s)	215, 1946
ScrFI	CCNGG	5 cut(s)	356, 753, 1142, 1663, 2026
SduI	GDGCHC	5 cut(s)	276, 702, 1179, 1619, 1947
SexAI	ACCWGGT	1 cut(s)	1140
SfaNI	GCATC	4 cut(s)	34, 441, 857, 1543
SfcI	CTRYAG	2 cut(s)	507, 1048
SfoI	GGCGCC	2 cut(s)	1071, 1387
SfuI	TTCGAA	1 cut(s)	1893
SinI	GGWCC	1 cut(s)	984
SmlI	CTYRAG	1 cut(s)	329
SmoI	CTYRAG	1 cut(s)	329
SpeI	ACTAGT	1 cut(s)	160
SseBI	AGGCCT	1 cut(s)	125
SspDI	GGCGCC	2 cut(s)	1069, 1385
SspI	AATATT	2 cut(s)	380, 2091
SspMI	CTAG	2 cut(s)	161, 1977
SstI	GAGCTC	1 cut(s)	276
StuI	AGGCCT	1 cut(s)	125
StyD4I	CCNGG	5 cut(s)	354, 751, 1140, 1661, 2024
TaaI	ACNGT	3 cut(s)	628, 1566, 2018
TaiI	ACGT	3 cut(s)	912, 922, 1722
TaqI	TCGA	5 cut(s)	606, 708, 828, 1149, 1893
TauI	GCSGC	2 cut(s)	798, 1225
TfiI	GAWTC	6 cut(s)	249, 756, 1018, 1839, 2021, 2063
Tru1I	TTAA	5 cut(s)	153, 417, 1350, 1455, 1464
Tru9I	TTAA	5 cut(s)	153, 417, 1350, 1455, 1464
TscAI	CASTG	2 cut(s)	453, 2023
TseFI	GTSAC	3 cut(s)	628, 1186, 1288
TseI	GCWGC	5 cut(s)	71, 111, 521, 870, 997
Tsp45I	GTSAC	3 cut(s)	628, 1186, 1288
TspDTI	ATGAA	4 cut(s)	171, 207, 294, 874
TspRI	CASTG	2 cut(s)	453, 2023
VpaK11BI	GGWCC	1 cut(s)	984
XagI	CCTNNNNNAGG	2 cut(s)	1164, 2042
XapI	RAATTY	2 cut(s)	959, 1807
XcmI	CCANNNNNNNNNTGG	1 cut(s)	645
XmiI	GTMKAC	1 cut(s)	1919
XmnI	GAANNNNTTC	2 cut(s)	162, 2076
XspI	CTAG	2 cut(s)	161, 1977
ZraI	GACGTC	1 cut(s)	910

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.