Rh1AG178000

tRNA (guanine-N(7)-)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000530
Physical Location & Seq
Forward (+)
33902511 .. 33908004
5494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG178000.1

Sequence Viewer

Length: 825 bp
ATGCCTGCCATGGGATTTGTGCCCATTTTGGGTTCCAAGAGAGTGTCTGGTGACTACATATACCATATTGATGGATGGGCTATTAAGGTTATGGATGAGATGGAGGATAATGGGGTTGGCGCCAATGAGGTTACTTATGGGGTTGTCATTGAAGCATATTGTAAGGAGAAGAAGTCGGGCGAAGCGATTAATTTGCTTAGTGATATGGTCGAGAAGACGTATCTACCGAGCTCGGTGCTCTGTTGTAAGGTGATTGATGTTTTGTGTTGCAAAGGAAAGGTGGAGGATGCTTGTGAACTGTGGAAGAGGCTTTTGAAGAAGAATTGCAAGCCGGCAGGCTTATTTCTGGAATGTGTGAAGTGGGGGAGTTGTGTGAGGCAGGGAGGTTGTGGGATGACATGGTGGAGAAGGGATGATGTGATCAAAGGATTCTGTAAGATTAGAAAAGCGAAGGAGCGAATTAGGATTTTGGACAAGATGTTCGAAAATGGCTGTTTGCCAAACAAGTCTACTTATGCTATGTTGATTGAGGGGCTCTGTGACTCGGGAGAGGAAGCTGAAATCACTAGATGCGGGAGTTGTGTGATGCTTGTCAAACGTGCTGATGTCTGGGTTAAAGAGCTGGGTCGTAGTAACATACATTTCATGTATGCAAATGCCACTACTTCATTTAATCAACTGGTGTCTACATACCCTGGACCACTGATGCTTGTTTCAATCCTGTGCCCAGATCCGTATTTTAAGAGAAAGCATCACAAGAGACGGGTTGTACAGAAGCCTTTAGTAGATTCCATTGTGAATAATTTAGCTCCCCGCGGACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_4

Protein Analysis

274

Amino Acids

30.85

Weight (kDa)

8.85

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 42 - 78 9e-07 PPR repeat family
PPR_2 PF13041 140 - 181 7.3e-09 PPR repeat family
Methyltransf_4 PF02390 204 - 274 2.3e-12 Putative methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000530)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16420 AT5G16420 AT5G16420 AT5G16420
fragaria_vesca FvH4_7g08341 FvH4_7g08360
malus_domestica MD02G1232100.v1.1 MD07G1081300.v1.1
prunus_persica Prupe.2G106500_v2.0.a1
pyrus_communis pycom02g20040
rosa_chinensis RchiOBHm_Chr1g0340331 RchiOBHm_Chr1g0340431 RchiOBHm_Chr1g0340441 RchiOBHm_Chr1g0340491 RchiOBHm_Chr1g0340531 RchiOBHm_Chr1g0340541 RchiOBHm_Chr1g0340561 RchiOBHm_Chr1g0340621 RchiOBHm_Chr1g0340661 RchiOBHm_Chr1g0340691
rosa_laevigata RLG00000029013 RLG00000029018 RLG00000029020 RLG00000029024 RLG00000029026 RLG00000029029 RLG00000029030 RLG00000029032 RLG00000029033
rosa_multiflora Rmu_sc0000566.1_g000026 Rmu_sc0000566.1_g000034 Rmu_sc0000566.1_g000054 Rmu_sc0001836.1_g000016 Rmu_sc0001836.1_g000022 Rmu_sc0001836.1_g000023 Rmu_sc0001836.1_g000028 Rmu_sc0001836.1_g000058 Rmu_sc0001946.1_g000004 Rmu_sc0002595.1_g000001 Rmu_sc0002595.1_g000005 Rmu_sc0005167.1_g000003 Rmu_sc0005167.1_g000009 Rmu_sc0005167.1_g000018 Rmu_sc0024761.1_g000004
rosa_roxburghii Rroxscaffold_4G00311170 Rroxscaffold_4G00311190 Rroxscaffold_4G00311250 Rroxscaffold_4G00315930 Rroxscaffold_4G00315960 Rroxscaffold_5G00353270
rosa_rugosa Rorug01G0162000.1 Rorug01G0162500.1 Rorug01G0162800.1 Rorug01G0163000.1 Rorug01G0163400.1 Rorug01G0163700.1 Rorug01G0164100.1 Rorug01G0164300 Rorug05G0484800
rosa_samantha Rh1AG177800 Rh1AG178000 Rh1AG178300 Rh1AG178600 Rh1AG178900 Rh1BG145300 Rh1BG145600 Rh1BG145800 Rh1BG145900 Rh1BG146000 Rh1BG146700 Rh1CG165100 Rh1CG165200 Rh1CG165400 Rh1CG165600 Rh1CG165700 Rh1CG165900 Rh1DG177500 Rh1DG177600 Rh1DG177800 Rh1DG177900 Rh1DG178000 Rh1DG178800
rosa_wichuraiana Rw0G006210 Rw1G014800 Rw1G014890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 119
AccI GTMKAC 2 cut(s) 509, 686
AccII CGCG 1 cut(s) 816
AciI CCGC 3 cut(s) 573, 814, 816
AclWI GGATC 1 cut(s) 725
AcyI GRCGYC 1 cut(s) 120
AfaI GTAC 1 cut(s) 771
AfiI CCNNNNNNNGG 2 cut(s) 11, 29
AgsI TTSAA 3 cut(s) 152, 316, 717
AjnI CCWGG 1 cut(s) 694
AloI GAACNNNNNNTCC 2 cut(s) 464, 496
AluBI AGCT 4 cut(s) 231, 557, 622, 809
AluI AGCT 4 cut(s) 231, 557, 622, 809
Alw21I GWGCWC 2 cut(s) 233, 240
Alw26I GTCTC 1 cut(s) 754
AlwI GGATC 1 cut(s) 725
Ama87I CYCGRG 1 cut(s) 544
ArsI GACNNNNNNTTYG 2 cut(s) 464, 496
AseI ATTAAT 1 cut(s) 189
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 698
AsuHPI GGTGA 2 cut(s) 62, 262
AsuII TTCGAA 1 cut(s) 483
AvaI CYCGRG 1 cut(s) 544
AvaII GGWCC 1 cut(s) 698
BaeGI GKGCMC 2 cut(s) 24, 728
BanI GGYRCC 1 cut(s) 119
BanII GRGCYC 2 cut(s) 233, 537
BbsI GAAGAC 1 cut(s) 221
Bbv12I GWGCWC 2 cut(s) 233, 240
BccI CCATC 3 cut(s) 65, 69, 94
BcgI CGANNNNNNTGC 4 cut(s) 175, 209, 217, 251
BciT130I CCWGG 1 cut(s) 696
BclI TGATCA 1 cut(s) 420
BcoDI GTCTC 1 cut(s) 754
BfaI CTAG 1 cut(s) 567
BfoI RGCGCY 1 cut(s) 123
Bme1390I CCNGG 1 cut(s) 696
Bme18I GGWCC 1 cut(s) 698
BmeT110I CYCGRG 1 cut(s) 544
BmgT120I GGNCC 1 cut(s) 698
BmiI GGNNCC 2 cut(s) 34, 121
BmrFI CCNGG 1 cut(s) 696
BmsI GCATC 5 cut(s) 277, 560, 576, 696, 760
BpiI GAAGAC 1 cut(s) 221
Bpu14I TTCGAA 1 cut(s) 483
BsaHI GRCGYC 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 9, 694, 814
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 2 cut(s) 11, 29
Bse118I RCCGGY 1 cut(s) 331
Bse1I ACTGG 1 cut(s) 684
BseBI CCWGG 1 cut(s) 696
BseDI CCNNGG 3 cut(s) 9, 694, 814
BseGI GGATG 5 cut(s) 80, 100, 292, 399, 418
BseLI CCNNNNNNNGG 2 cut(s) 11, 29
BseNI ACTGG 1 cut(s) 684
BseSI GKGCMC 2 cut(s) 24, 728
BseYI CCCAGC 1 cut(s) 622
Bsh1236I CGCG 1 cut(s) 816
BshNI GGYRCC 1 cut(s) 119
BsiHKAI GWGCWC 2 cut(s) 233, 240
BsiHKCI CYCGRG 1 cut(s) 544
BsiSI CCGG 1 cut(s) 332
BslI CCNNNNNNNGG 2 cut(s) 11, 29
BsmAI GTCTC 1 cut(s) 754
BsmBI CGTCTC 1 cut(s) 754
BsoBI CYCGRG 1 cut(s) 544
Bsp119I TTCGAA 1 cut(s) 483
Bsp1286I GDGCHC 5 cut(s) 24, 233, 240, 537, 728
Bsp1407I TGTACA 1 cut(s) 769
Bsp143I GATC 2 cut(s) 420, 730
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 3 cut(s) 573, 814, 816
BspFNI CGCG 1 cut(s) 816
BspLI GGNNCC 2 cut(s) 34, 121
BspPI GGATC 1 cut(s) 725
BspT104I TTCGAA 1 cut(s) 483
BspT107I GGYRCC 1 cut(s) 119
BsrFI RCCGGY 1 cut(s) 331
BsrGI TGTACA 1 cut(s) 769
BsrI ACTGG 1 cut(s) 684
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 3 cut(s) 9, 694, 814
BssMI GATC 2 cut(s) 420, 730
BssNI GRCGYC 1 cut(s) 120
BssT1I CCWWGG 1 cut(s) 9
Bst2UI CCWGG 1 cut(s) 696
Bst4CI ACNGT 2 cut(s) 300, 822
Bst6I CTCTTC 1 cut(s) 299
BstACI GRCGYC 1 cut(s) 120
BstAUI TGTACA 1 cut(s) 769
BstBI TTCGAA 1 cut(s) 483
BstC8I GCNNGC 4 cut(s) 6, 329, 333, 337
BstDEI CTNAG 1 cut(s) 197
BstDSI CCRYGG 2 cut(s) 9, 814
BstF5I GGATG 5 cut(s) 80, 100, 292, 399, 418
BstFNI CGCG 1 cut(s) 816
BstH2I RGCGCY 1 cut(s) 123
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 2 cut(s) 423, 733
BstMAI GTCTC 1 cut(s) 754
BstMBI GATC 2 cut(s) 420, 730
BstNI CCWGG 1 cut(s) 696
BstSCI CCNGG 1 cut(s) 694
BstSLI GKGCMC 2 cut(s) 24, 728
BstUI CGCG 1 cut(s) 816
BstV2I GAAGAC 1 cut(s) 221
BstX2I RGATCY 1 cut(s) 730
BstXI CCANNNNNNTGG 1 cut(s) 71
BstYI RGATCY 1 cut(s) 730
BtgI CCRYGG 2 cut(s) 9, 814
BtsCI GGATG 5 cut(s) 80, 100, 292, 399, 418
BtsIMutI CAGTG 1 cut(s) 701
Cac8I GCNNGC 4 cut(s) 6, 329, 333, 337
CfoI GCGC 1 cut(s) 122
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 1 cut(s) 698
Cfr42I CCGCGG 1 cut(s) 817
Csp6I GTAC 1 cut(s) 770
CviAII CATG 3 cut(s) 10, 399, 646
CviQI GTAC 1 cut(s) 770
DdeI CTNAG 1 cut(s) 197
DinI GGCGCC 1 cut(s) 121
DpnI GATC 2 cut(s) 422, 732
DpnII GATC 2 cut(s) 420, 730
Eam1104I CTCTTC 1 cut(s) 299
EarI CTCTTC 1 cut(s) 299
Ecl136II GAGCTC 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 9
Eco24I GRGCYC 2 cut(s) 233, 537
Eco47I GGWCC 1 cut(s) 698
Eco53kI GAGCTC 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 544
EcoICRI GAGCTC 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 694
EcoT14I CCWWGG 1 cut(s) 9
EcoT38I GRGCYC 2 cut(s) 233, 537
EgeI GGCGCC 1 cut(s) 121
EheI GGCGCC 1 cut(s) 121
ErhI CCWWGG 1 cut(s) 9
Esp3I CGTCTC 1 cut(s) 754
FaeI CATG 3 cut(s) 13, 402, 649
FatI CATG 3 cut(s) 9, 398, 645
FauI CCCGC 2 cut(s) 566, 821
FbaI TGATCA 1 cut(s) 420
FblI GTMKAC 2 cut(s) 509, 686
FokI GGATG 5 cut(s) 87, 107, 299, 406, 425
FriOI GRGCYC 2 cut(s) 233, 537
FspBI CTAG 1 cut(s) 567
GlaI GCGC 1 cut(s) 121
GsaI CCCAGC 1 cut(s) 626
HaeII RGCGCY 1 cut(s) 123
HapII CCGG 1 cut(s) 332
HhaI GCGC 1 cut(s) 122
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 3 cut(s) 13, 402, 649
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HinfI GANTC 3 cut(s) 429, 542, 788
HpaII CCGG 1 cut(s) 332
HphI GGTGA 2 cut(s) 62, 262
Hpy166II GTNNAC 3 cut(s) 296, 510, 687
Hpy188III TCNNGA 3 cut(s) 211, 347, 546
Hpy8I GTNNAC 3 cut(s) 296, 510, 687
HpyAV CCTTC 2 cut(s) 402, 445
HpyCH4III ACNGT 2 cut(s) 300, 822
HpyCH4IV ACGT 2 cut(s) 218, 598
HpyCH4V TGCA 3 cut(s) 270, 327, 653
HpyF3I CTNAG 1 cut(s) 197
HpySE526I ACGT 2 cut(s) 218, 598
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 3 cut(s) 13, 402, 649
HspAI GCGC 1 cut(s) 120
KasI GGCGCC 1 cut(s) 119
KroI GCCGGC 1 cut(s) 331
KroNI GCCGGC 1 cut(s) 333
Ksp22I TGATCA 1 cut(s) 420
KspI CCGCGG 1 cut(s) 817
Kzo9I GATC 2 cut(s) 420, 730
LmnI GCTCC 2 cut(s) 454, 814
LweI GCATC 5 cut(s) 277, 560, 576, 696, 760
MaeI CTAG 1 cut(s) 567
MaeII ACGT 2 cut(s) 218, 598
MaeIII GTNAC 4 cut(s) 50, 130, 539, 632
MalI GATC 2 cut(s) 422, 732
MboI GATC 2 cut(s) 420, 730
MboII GAAGA 5 cut(s) 181, 226, 316, 328, 331
MflI RGATCY 1 cut(s) 730
MhlI GDGCHC 5 cut(s) 24, 233, 240, 537, 728
MluCI AATT 4 cut(s) 190, 322, 459, 802
Mly113I GGCGCC 1 cut(s) 120
MlyI GAGTC 1 cut(s) 536
MnlI CCTC 8 cut(s) 97, 121, 277, 300, 369, 377, 523, 544
MroNI GCCGGC 1 cut(s) 331
MseI TTAA 5 cut(s) 84, 189, 615, 672, 741
MslI CAYNNNNRTG 1 cut(s) 69
MspA1I CMGCKG 1 cut(s) 816
MspI CCGG 1 cut(s) 332
MspR9I CCNGG 1 cut(s) 696
MvaI CCWGG 1 cut(s) 696
MvnI CGCG 1 cut(s) 816
NaeI GCCGGC 1 cut(s) 333
NarI GGCGCC 1 cut(s) 120
NcoI CCATGG 1 cut(s) 9
NdeII GATC 2 cut(s) 420, 730
NgoMIV GCCGGC 1 cut(s) 331
NlaIII CATG 3 cut(s) 13, 402, 649
NlaIV GGNNCC 2 cut(s) 34, 121
NmuCI GTSAC 2 cut(s) 50, 539
NspV TTCGAA 1 cut(s) 483
PcsI WCGNNNNNNNCGW 2 cut(s) 182, 224
PdiI GCCGGC 1 cut(s) 333
PfeI GAWTC 2 cut(s) 429, 788
PleI GAGTC 1 cut(s) 536
PluTI GGCGCC 1 cut(s) 123
PpsI GAGTC 1 cut(s) 536
PshBI ATTAAT 1 cut(s) 189
Psp124BI GAGCTC 1 cut(s) 233
Psp6I CCWGG 1 cut(s) 694
PspFI CCCAGC 1 cut(s) 622
PspGI CCWGG 1 cut(s) 694
PspN4I GGNNCC 2 cut(s) 34, 121
PspPI GGNCC 1 cut(s) 698
PsuI RGATCY 1 cut(s) 730
RsaI GTAC 1 cut(s) 771
RsaNI GTAC 1 cut(s) 770
RseI CAYNNNNRTG 1 cut(s) 69
SacI GAGCTC 1 cut(s) 233
SacII CCGCGG 1 cut(s) 817
SaqAI TTAA 5 cut(s) 84, 189, 615, 672, 741
Sau3AI GATC 2 cut(s) 420, 730
Sau96I GGNCC 1 cut(s) 698
SchI GAGTC 1 cut(s) 536
ScrFI CCNGG 1 cut(s) 696
SduI GDGCHC 5 cut(s) 24, 233, 240, 537, 728
SfaNI GCATC 5 cut(s) 277, 560, 576, 696, 760
SfoI GGCGCC 1 cut(s) 121
Sfr303I CCGCGG 1 cut(s) 817
SfuI TTCGAA 1 cut(s) 483
SgrBI CCGCGG 1 cut(s) 817
SinI GGWCC 1 cut(s) 698
SmiMI CAYNNNNRTG 1 cut(s) 69
Sse9I AATT 4 cut(s) 190, 322, 459, 802
SsiI CCGC 3 cut(s) 573, 814, 816
SspDI GGCGCC 1 cut(s) 119
SspMI CTAG 1 cut(s) 567
SstI GAGCTC 1 cut(s) 233
StyD4I CCNGG 1 cut(s) 694
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 2 cut(s) 300, 822
TaiI ACGT 2 cut(s) 221, 601
TaqI TCGA 2 cut(s) 210, 483
TasI AATT 4 cut(s) 190, 322, 459, 802
TatI WGTACW 1 cut(s) 769
TfiI GAWTC 2 cut(s) 429, 788
Tru1I TTAA 5 cut(s) 84, 189, 615, 672, 741
Tru9I TTAA 5 cut(s) 84, 189, 615, 672, 741
TscAI CASTG 1 cut(s) 708
TseFI GTSAC 2 cut(s) 50, 539
Tsp45I GTSAC 2 cut(s) 50, 539
TspDTI ATGAA 2 cut(s) 634, 657
TspGWI ACGGA 1 cut(s) 723
TspRI CASTG 1 cut(s) 708
VpaK11BI GGWCC 1 cut(s) 698
VspI ATTAAT 1 cut(s) 189
XmiI GTMKAC 2 cut(s) 509, 686
XspI CTAG 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.