Rh1CG165100

tRNA (guanine-N(7)-)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000530
Physical Location & Seq
Forward (+)
36401586 .. 36406750
5165 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG165100.1

Sequence Viewer

Length: 1011 bp
ATGCCTGCCATGGGATTTGTGCCCATTTTGGGTTCCAAGAGAGTGTCTGGTGACTACATATACCATATTGATGGATGGGCTATTAAGGTTATGGATGAGATGGAGGATAATGGGGTTGGCGCCAATGAGGTTACTTATGGGGTTGTCATTGAAGCATATTGTAAGGAGAAGAAGTCGGGCGAAGCGATTAATTTGCTTAGTGATATGGTCGAGAAGACGTATCTACCGAGCTCGGTGCTCTGTTGTAAGGTGATTGATGTTTTGTGTTGCAAAGGAAAGGTGGAGGATGCTTGTGAACTGTGGAAGAGGCTTTTGAAGAAGAATTGCAAGCCGGCAGGCTTATTTCTGGAATGTGTGAAGTGGGGGAGTTGTGTGAGGCAGGGAGGTTGTGGGATGACATGGTGGAGAAGGGATGATGTGATCAAAGGATTCTGTAAGATTAGAAAAGCGAAGGAGCGAATTAGGATTTTGGACAAGATGTTCGAAAATGGCTGTTTGCCAAACAAGTCTACTTATGCTATGTTGATTGAGGGGCTCTGTGACTCGGGAGAGGAAGCTGAAATCACTAGAGGGAAGCTTGTTGAGGCTATTAAGGTTATGGATGAGATGGAGGATAATGGGGTTGGCGCCAATGAGGTTACTTATGGGGTTATGATTGAAGCTTATTGTAAGGAGAAGAAGTCGGGCGAAGCGTGCGGGAGTTGTGTGATGCTTGTCAAACGTGCTGATGTCTGGGTTAAAGAGCTGGGTCGTAGTAACATACATTTCATGTATGCAAATGCCACTACTTCATTTAATCAACTGGTGTCTACATACCCTGGACCACTGATGCTTGTTTCAATCCTGTGCCCAGATCCGTATTTTAAGAGAAAGCATCACAAGAGACGGGTTGTACAGAAGCCTTTAGTAGATTCCATTGTGAATAATTTAGCTCCCCGCGGACAGTTTCTTTATTCAAAGGAGGAAAAAAAGGACCATAACATCAATAGAGAAAAAATATACTTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_4

Protein Analysis

336

Amino Acids

38.02

Weight (kDa)

8.54

Isoelectric Point (pI)

43.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 42 - 78 1.2e-06 PPR repeat family
PPR_2 PF13041 140 - 181 9.8e-09 PPR repeat family
PPR_2 PF13041 189 - 224 3.8e-08 PPR repeat family
Methyltransf_4 PF02390 245 - 316 8.8e-13 Putative methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000530)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16420 AT5G16420 AT5G16420 AT5G16420
fragaria_vesca FvH4_7g08341 FvH4_7g08360
malus_domestica MD02G1232100.v1.1 MD07G1081300.v1.1
prunus_persica Prupe.2G106500_v2.0.a1
pyrus_communis pycom02g20040
rosa_chinensis RchiOBHm_Chr1g0340331 RchiOBHm_Chr1g0340431 RchiOBHm_Chr1g0340441 RchiOBHm_Chr1g0340491 RchiOBHm_Chr1g0340531 RchiOBHm_Chr1g0340541 RchiOBHm_Chr1g0340561 RchiOBHm_Chr1g0340621 RchiOBHm_Chr1g0340661 RchiOBHm_Chr1g0340691
rosa_laevigata RLG00000029013 RLG00000029018 RLG00000029020 RLG00000029024 RLG00000029026 RLG00000029029 RLG00000029030 RLG00000029032 RLG00000029033
rosa_multiflora Rmu_sc0000566.1_g000026 Rmu_sc0000566.1_g000034 Rmu_sc0000566.1_g000054 Rmu_sc0001836.1_g000016 Rmu_sc0001836.1_g000022 Rmu_sc0001836.1_g000023 Rmu_sc0001836.1_g000028 Rmu_sc0001836.1_g000058 Rmu_sc0001946.1_g000004 Rmu_sc0002595.1_g000001 Rmu_sc0002595.1_g000005 Rmu_sc0005167.1_g000003 Rmu_sc0005167.1_g000009 Rmu_sc0005167.1_g000018 Rmu_sc0024761.1_g000004
rosa_roxburghii Rroxscaffold_4G00311170 Rroxscaffold_4G00311190 Rroxscaffold_4G00311250 Rroxscaffold_4G00315930 Rroxscaffold_4G00315960 Rroxscaffold_5G00353270
rosa_rugosa Rorug01G0162000.1 Rorug01G0162500.1 Rorug01G0162800.1 Rorug01G0163000.1 Rorug01G0163400.1 Rorug01G0163700.1 Rorug01G0164100.1 Rorug01G0164300 Rorug05G0484800
rosa_samantha Rh1AG177800 Rh1AG178000 Rh1AG178300 Rh1AG178600 Rh1AG178900 Rh1BG145300 Rh1BG145600 Rh1BG145800 Rh1BG145900 Rh1BG146000 Rh1BG146700 Rh1CG165100 Rh1CG165200 Rh1CG165400 Rh1CG165600 Rh1CG165700 Rh1CG165900 Rh1DG177500 Rh1DG177600 Rh1DG177800 Rh1DG177900 Rh1DG178000 Rh1DG178800
rosa_wichuraiana Rw0G006210 Rw1G014800 Rw1G014890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 119, 626
AccI GTMKAC 2 cut(s) 509, 809
AccII CGCG 1 cut(s) 939
AciI CCGC 3 cut(s) 696, 937, 939
AclWI GGATC 1 cut(s) 848
AcyI GRCGYC 2 cut(s) 120, 627
AfaI GTAC 1 cut(s) 894
AfiI CCNNNNNNNGG 2 cut(s) 11, 29
AgsI TTSAA 5 cut(s) 152, 316, 659, 840, 957
AjnI CCWGG 1 cut(s) 817
AloI GAACNNNNNNTCC 2 cut(s) 464, 496
AluBI AGCT 6 cut(s) 231, 557, 577, 662, 745, 932
AluI AGCT 6 cut(s) 231, 557, 577, 662, 745, 932
Alw21I GWGCWC 2 cut(s) 233, 240
Alw26I GTCTC 1 cut(s) 877
AlwI GGATC 1 cut(s) 848
Ama87I CYCGRG 1 cut(s) 544
ArsI GACNNNNNNTTYG 2 cut(s) 464, 496
AseI ATTAAT 1 cut(s) 189
AspLEI GCGC 2 cut(s) 122, 629
AspS9I GGNCC 2 cut(s) 821, 973
AsuHPI GGTGA 2 cut(s) 62, 262
AsuII TTCGAA 1 cut(s) 483
AvaI CYCGRG 1 cut(s) 544
AvaII GGWCC 2 cut(s) 821, 973
BaeGI GKGCMC 2 cut(s) 24, 851
BanI GGYRCC 2 cut(s) 119, 626
BanII GRGCYC 2 cut(s) 233, 537
BbsI GAAGAC 1 cut(s) 221
Bbv12I GWGCWC 2 cut(s) 233, 240
BccI CCATC 4 cut(s) 65, 69, 94, 601
BcgI CGANNNNNNTGC 4 cut(s) 175, 209, 217, 251
BciT130I CCWGG 1 cut(s) 819
BclI TGATCA 1 cut(s) 420
BcoDI GTCTC 1 cut(s) 877
BfaI CTAG 1 cut(s) 567
BfoI RGCGCY 2 cut(s) 123, 630
Bme1390I CCNGG 1 cut(s) 819
Bme18I GGWCC 2 cut(s) 821, 973
BmeT110I CYCGRG 1 cut(s) 544
BmgT120I GGNCC 2 cut(s) 821, 973
BmiI GGNNCC 3 cut(s) 34, 121, 628
BmrFI CCNGG 1 cut(s) 819
BmsI GCATC 4 cut(s) 277, 699, 819, 883
BpiI GAAGAC 1 cut(s) 221
Bpu14I TTCGAA 1 cut(s) 483
BsaHI GRCGYC 2 cut(s) 120, 627
BsaJI CCNNGG 3 cut(s) 9, 817, 937
BsaXI ACNNNNNCTCC 4 cut(s) 158, 188, 665, 695
Bsc4I CCNNNNNNNGG 2 cut(s) 11, 29
Bse118I RCCGGY 1 cut(s) 331
Bse1I ACTGG 1 cut(s) 807
BseBI CCWGG 1 cut(s) 819
BseDI CCNNGG 3 cut(s) 9, 817, 937
BseGI GGATG 6 cut(s) 80, 100, 292, 399, 418, 607
BseLI CCNNNNNNNGG 2 cut(s) 11, 29
BseNI ACTGG 1 cut(s) 807
BseSI GKGCMC 2 cut(s) 24, 851
BseYI CCCAGC 1 cut(s) 745
Bsh1236I CGCG 1 cut(s) 939
BshNI GGYRCC 2 cut(s) 119, 626
BsiHKAI GWGCWC 2 cut(s) 233, 240
BsiHKCI CYCGRG 1 cut(s) 544
BsiSI CCGG 1 cut(s) 332
BslI CCNNNNNNNGG 2 cut(s) 11, 29
BsmAI GTCTC 1 cut(s) 877
BsmBI CGTCTC 1 cut(s) 877
BsoBI CYCGRG 1 cut(s) 544
Bsp119I TTCGAA 1 cut(s) 483
Bsp1286I GDGCHC 5 cut(s) 24, 233, 240, 537, 851
Bsp1407I TGTACA 1 cut(s) 892
Bsp143I GATC 2 cut(s) 420, 853
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 3 cut(s) 696, 937, 939
BspFNI CGCG 1 cut(s) 939
BspLI GGNNCC 3 cut(s) 34, 121, 628
BspPI GGATC 1 cut(s) 848
BspT104I TTCGAA 1 cut(s) 483
BspT107I GGYRCC 2 cut(s) 119, 626
BsrFI RCCGGY 1 cut(s) 331
BsrGI TGTACA 1 cut(s) 892
BsrI ACTGG 1 cut(s) 807
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 3 cut(s) 9, 817, 937
BssMI GATC 2 cut(s) 420, 853
BssNI GRCGYC 2 cut(s) 120, 627
BssT1I CCWWGG 1 cut(s) 9
Bst2UI CCWGG 1 cut(s) 819
Bst4CI ACNGT 2 cut(s) 300, 945
Bst6I CTCTTC 1 cut(s) 299
BstACI GRCGYC 2 cut(s) 120, 627
BstAUI TGTACA 1 cut(s) 892
BstBI TTCGAA 1 cut(s) 483
BstC8I GCNNGC 5 cut(s) 6, 329, 333, 337, 694
BstDEI CTNAG 1 cut(s) 197
BstDSI CCRYGG 2 cut(s) 9, 937
BstF5I GGATG 6 cut(s) 80, 100, 292, 399, 418, 607
BstFNI CGCG 1 cut(s) 939
BstH2I RGCGCY 2 cut(s) 123, 630
BstHHI GCGC 2 cut(s) 122, 629
BstKTI GATC 2 cut(s) 423, 856
BstMAI GTCTC 1 cut(s) 877
BstMBI GATC 2 cut(s) 420, 853
BstMWI GCNNNNNNNGC 1 cut(s) 693
BstNI CCWGG 1 cut(s) 819
BstSCI CCNGG 1 cut(s) 817
BstSLI GKGCMC 2 cut(s) 24, 851
BstUI CGCG 1 cut(s) 939
BstV2I GAAGAC 1 cut(s) 221
BstX2I RGATCY 1 cut(s) 853
BstXI CCANNNNNNTGG 1 cut(s) 71
BstYI RGATCY 1 cut(s) 853
BtgI CCRYGG 2 cut(s) 9, 937
BtsCI GGATG 6 cut(s) 80, 100, 292, 399, 418, 607
BtsIMutI CAGTG 1 cut(s) 824
Cac8I GCNNGC 5 cut(s) 6, 329, 333, 337, 694
CfoI GCGC 2 cut(s) 122, 629
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 2 cut(s) 821, 973
Cfr42I CCGCGG 1 cut(s) 940
Csp6I GTAC 1 cut(s) 893
CviAII CATG 3 cut(s) 10, 399, 769
CviQI GTAC 1 cut(s) 893
DdeI CTNAG 1 cut(s) 197
DinI GGCGCC 2 cut(s) 121, 628
DpnI GATC 2 cut(s) 422, 855
DpnII GATC 2 cut(s) 420, 853
Eam1104I CTCTTC 1 cut(s) 299
EarI CTCTTC 1 cut(s) 299
Ecl136II GAGCTC 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 9
Eco24I GRGCYC 2 cut(s) 233, 537
Eco47I GGWCC 2 cut(s) 821, 973
Eco53kI GAGCTC 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 544
EcoICRI GAGCTC 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 817
EcoT14I CCWWGG 1 cut(s) 9
EcoT38I GRGCYC 2 cut(s) 233, 537
EgeI GGCGCC 2 cut(s) 121, 628
EheI GGCGCC 2 cut(s) 121, 628
ErhI CCWWGG 1 cut(s) 9
Esp3I CGTCTC 1 cut(s) 877
FaeI CATG 3 cut(s) 13, 402, 772
FatI CATG 3 cut(s) 9, 398, 768
FauI CCCGC 2 cut(s) 689, 944
FbaI TGATCA 1 cut(s) 420
FblI GTMKAC 2 cut(s) 509, 809
FokI GGATG 6 cut(s) 87, 107, 299, 406, 425, 614
FriOI GRGCYC 2 cut(s) 233, 537
FspBI CTAG 1 cut(s) 567
GlaI GCGC 2 cut(s) 121, 628
GsaI CCCAGC 1 cut(s) 749
HaeII RGCGCY 2 cut(s) 123, 630
HapII CCGG 1 cut(s) 332
HhaI GCGC 2 cut(s) 122, 629
Hin1I GRCGYC 2 cut(s) 120, 627
Hin1II CATG 3 cut(s) 13, 402, 772
Hin6I GCGC 2 cut(s) 120, 627
HinP1I GCGC 2 cut(s) 120, 627
HindIII AAGCTT 2 cut(s) 575, 660
HinfI GANTC 3 cut(s) 429, 542, 911
HpaII CCGG 1 cut(s) 332
HphI GGTGA 2 cut(s) 62, 262
Hpy166II GTNNAC 3 cut(s) 296, 510, 810
Hpy188III TCNNGA 3 cut(s) 211, 347, 546
Hpy8I GTNNAC 3 cut(s) 296, 510, 810
HpyAV CCTTC 2 cut(s) 402, 445
HpyCH4III ACNGT 2 cut(s) 300, 945
HpyCH4IV ACGT 2 cut(s) 218, 721
HpyCH4V TGCA 3 cut(s) 270, 327, 776
HpyF10VI GCNNNNNNNGC 1 cut(s) 693
HpyF3I CTNAG 1 cut(s) 197
HpySE526I ACGT 2 cut(s) 218, 721
Hsp92I GRCGYC 2 cut(s) 120, 627
Hsp92II CATG 3 cut(s) 13, 402, 772
HspAI GCGC 2 cut(s) 120, 627
KasI GGCGCC 2 cut(s) 119, 626
KroI GCCGGC 1 cut(s) 331
KroNI GCCGGC 1 cut(s) 333
Ksp22I TGATCA 1 cut(s) 420
KspI CCGCGG 1 cut(s) 940
Kzo9I GATC 2 cut(s) 420, 853
LmnI GCTCC 2 cut(s) 454, 937
LweI GCATC 4 cut(s) 277, 699, 819, 883
MaeI CTAG 1 cut(s) 567
MaeII ACGT 2 cut(s) 218, 721
MaeIII GTNAC 5 cut(s) 50, 130, 539, 637, 755
MalI GATC 2 cut(s) 422, 855
MboI GATC 2 cut(s) 420, 853
MboII GAAGA 6 cut(s) 181, 226, 316, 328, 331, 688
MflI RGATCY 1 cut(s) 853
MhlI GDGCHC 5 cut(s) 24, 233, 240, 537, 851
MluCI AATT 4 cut(s) 190, 322, 459, 925
Mly113I GGCGCC 2 cut(s) 120, 627
MlyI GAGTC 1 cut(s) 536
MroNI GCCGGC 1 cut(s) 331
MseI TTAA 6 cut(s) 84, 189, 591, 738, 795, 864
MslI CAYNNNNRTG 1 cut(s) 69
MspA1I CMGCKG 1 cut(s) 939
MspI CCGG 1 cut(s) 332
MspR9I CCNGG 1 cut(s) 819
MvaI CCWGG 1 cut(s) 819
MvnI CGCG 1 cut(s) 939
MwoI GCNNNNNNNGC 1 cut(s) 693
NaeI GCCGGC 1 cut(s) 333
NarI GGCGCC 2 cut(s) 120, 627
NcoI CCATGG 1 cut(s) 9
NdeII GATC 2 cut(s) 420, 853
NgoMIV GCCGGC 1 cut(s) 331
NlaIII CATG 3 cut(s) 13, 402, 772
NlaIV GGNNCC 3 cut(s) 34, 121, 628
NmuCI GTSAC 2 cut(s) 50, 539
NspV TTCGAA 1 cut(s) 483
PcsI WCGNNNNNNNCGW 3 cut(s) 182, 224, 689
PdiI GCCGGC 1 cut(s) 333
PfeI GAWTC 2 cut(s) 429, 911
PleI GAGTC 1 cut(s) 536
PluTI GGCGCC 2 cut(s) 123, 630
PpsI GAGTC 1 cut(s) 536
PshBI ATTAAT 1 cut(s) 189
Psp124BI GAGCTC 1 cut(s) 233
Psp6I CCWGG 1 cut(s) 817
PspFI CCCAGC 1 cut(s) 745
PspGI CCWGG 1 cut(s) 817
PspN4I GGNNCC 3 cut(s) 34, 121, 628
PspPI GGNCC 2 cut(s) 821, 973
PsuI RGATCY 1 cut(s) 853
RsaI GTAC 1 cut(s) 894
RsaNI GTAC 1 cut(s) 893
RseI CAYNNNNRTG 1 cut(s) 69
SacI GAGCTC 1 cut(s) 233
SacII CCGCGG 1 cut(s) 940
SaqAI TTAA 6 cut(s) 84, 189, 591, 738, 795, 864
Sau3AI GATC 2 cut(s) 420, 853
Sau96I GGNCC 2 cut(s) 821, 973
SchI GAGTC 1 cut(s) 536
ScrFI CCNGG 1 cut(s) 819
SduI GDGCHC 5 cut(s) 24, 233, 240, 537, 851
SfaNI GCATC 4 cut(s) 277, 699, 819, 883
SfoI GGCGCC 2 cut(s) 121, 628
Sfr303I CCGCGG 1 cut(s) 940
SfuI TTCGAA 1 cut(s) 483
SgrBI CCGCGG 1 cut(s) 940
SinI GGWCC 2 cut(s) 821, 973
SmiMI CAYNNNNRTG 1 cut(s) 69
Sse9I AATT 4 cut(s) 190, 322, 459, 925
SsiI CCGC 3 cut(s) 696, 937, 939
SspDI GGCGCC 2 cut(s) 119, 626
SspMI CTAG 1 cut(s) 567
SstI GAGCTC 1 cut(s) 233
StyD4I CCNGG 1 cut(s) 817
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 2 cut(s) 300, 945
TaiI ACGT 2 cut(s) 221, 724
TaqI TCGA 2 cut(s) 210, 483
TasI AATT 4 cut(s) 190, 322, 459, 925
TatI WGTACW 1 cut(s) 892
TfiI GAWTC 2 cut(s) 429, 911
Tru1I TTAA 6 cut(s) 84, 189, 591, 738, 795, 864
Tru9I TTAA 6 cut(s) 84, 189, 591, 738, 795, 864
TscAI CASTG 1 cut(s) 831
TseFI GTSAC 2 cut(s) 50, 539
Tsp45I GTSAC 2 cut(s) 50, 539
TspDTI ATGAA 2 cut(s) 757, 780
TspGWI ACGGA 1 cut(s) 846
TspRI CASTG 1 cut(s) 831
VpaK11BI GGWCC 2 cut(s) 821, 973
VspI ATTAAT 1 cut(s) 189
XmiI GTMKAC 2 cut(s) 509, 809
XspI CTAG 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.