ribosome biogenesis protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 2954, 3195 |
| AarI | CACCTGC | 2 cut(s) | 881, 3025 |
| Acc36I | ACCTGC | 2 cut(s) | 881, 3025 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 382 |
| AccII | CGCG | 2 cut(s) | 1995, 3162 |
| AccIII | TCCGGA | 2 cut(s) | 2397, 2466 |
| AciI | CCGC | 9 cut(s) | 370, 420, 850, 1736, 2361, 2608, 2853, 3274, 3674 |
| AclWI | GGATC | 4 cut(s) | 929, 1029, 1450, 2855 |
| AcsI | RAATTY | 6 cut(s) | 425, 1680, 1937, 2147, 2818, 3474 |
| AcuI | CTGAAG | 2 cut(s) | 1647, 2399 |
| AfaI | GTAC | 2 cut(s) | 2738, 3369 |
| AfeI | AGCGCT | 1 cut(s) | 803 |
| AflII | CTTAAG | 1 cut(s) | 2732 |
| AflIII | ACRYGT | 2 cut(s) | 1507, 2323 |
| AhlI | ACTAGT | 1 cut(s) | 2911 |
| AjnI | CCWGG | 3 cut(s) | 1086, 2597, 3233 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 1214, 1246, 1296, 1328, 1715, 1747 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 733, 765 |
| Alw21I | GWGCWC | 1 cut(s) | 3119 |
| Alw26I | GTCTC | 8 cut(s) | 161, 168, 1682, 1747, 1872, 2132, 3264, 3661 |
| Alw44I | GTGCAC | 1 cut(s) | 3115 |
| AlwI | GGATC | 4 cut(s) | 929, 1029, 1450, 2855 |
| Aor13HI | TCCGGA | 2 cut(s) | 2397, 2466 |
| Aor51HI | AGCGCT | 1 cut(s) | 803 |
| AoxI | GGCC | 6 cut(s) | 72, 337, 1868, 2052, 2595, 2776 |
| ApaI | GGGCCC | 1 cut(s) | 2780 |
| ApaLI | GTGCAC | 1 cut(s) | 3115 |
| ApeKI | GCWGC | 6 cut(s) | 184, 589, 956, 973, 2027, 3047 |
| ApoI | RAATTY | 6 cut(s) | 425, 1680, 1937, 2147, 2818, 3474 |
| AsiSI | GCGATCGC | 1 cut(s) | 1999 |
| AspLEI | GCGC | 7 cut(s) | 804, 1995, 2190, 3162, 3164, 3531, 3606 |
| AspS9I | GGNCC | 6 cut(s) | 51, 257, 338, 373, 2776, 2777 |
| AsuC2I | CCSGG | 3 cut(s) | 55, 3029, 3635 |
| AsuII | TTCGAA | 1 cut(s) | 3010 |
| AvaII | GGWCC | 3 cut(s) | 51, 257, 373 |
| AxyI | CCTNAGG | 1 cut(s) | 3399 |
| BaeGI | GKGCMC | 3 cut(s) | 392, 2780, 3119 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2310, 2343 |
| BanII | GRGCYC | 1 cut(s) | 2780 |
| BauI | CACGAG | 1 cut(s) | 165 |
| BbsI | GAAGAC | 3 cut(s) | 1148, 1653, 3371 |
| Bbv12I | GWGCWC | 1 cut(s) | 3119 |
| BbvCI | CCTCAGC | 1 cut(s) | 952 |
| BbvI | GCAGC | 6 cut(s) | 196, 601, 943, 960, 2014, 3034 |
| BceAI | ACGGC | 3 cut(s) | 155, 238, 2874 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1610, 1644 |
| BciT130I | CCWGG | 3 cut(s) | 1088, 2599, 3235 |
| BciVI | GTATCC | 2 cut(s) | 2579, 3397 |
| BclI | TGATCA | 2 cut(s) | 285, 1063 |
| BcnI | CCSGG | 3 cut(s) | 55, 3029, 3635 |
| BcoDI | GTCTC | 8 cut(s) | 161, 168, 1682, 1747, 1872, 2132, 3264, 3661 |
| BcuI | ACTAGT | 1 cut(s) | 2911 |
| BfaI | CTAG | 3 cut(s) | 761, 1437, 2912 |
| BfmI | CTRYAG | 1 cut(s) | 3252 |
| BfoI | RGCGCY | 2 cut(s) | 805, 3607 |
| BfrI | CTTAAG | 1 cut(s) | 2732 |
| BfuAI | ACCTGC | 2 cut(s) | 881, 3025 |
| BfuI | GTATCC | 2 cut(s) | 2579, 3397 |
| BglII | AGATCT | 2 cut(s) | 1255, 2974 |
| BisI | GCNGC | 9 cut(s) | 185, 420, 590, 957, 974, 2028, 2362, 2609, 3048 |
| BlpI | GCTNAGC | 1 cut(s) | 928 |
| BlsI | GCNGC | 9 cut(s) | 186, 421, 591, 958, 975, 2029, 2363, 2610, 3049 |
| Bme1390I | CCNGG | 6 cut(s) | 55, 1088, 2599, 3029, 3235, 3635 |
| Bme18I | GGWCC | 3 cut(s) | 51, 257, 373 |
| BmgT120I | GGNCC | 6 cut(s) | 51, 257, 338, 373, 2776, 2777 |
| BmiI | GGNNCC | 7 cut(s) | 53, 67, 374, 2310, 2777, 2778, 3459 |
| BmrFI | CCNGG | 6 cut(s) | 55, 1088, 2599, 3029, 3235, 3635 |
| BpiI | GAAGAC | 3 cut(s) | 1148, 1653, 3371 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 730, 762, 3445, 3477 |
| BpmI | CTGGAG | 2 cut(s) | 143, 2727 |
| Bpu10I | CCTNAGC | 1 cut(s) | 952 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 928 |
| Bpu14I | TTCGAA | 1 cut(s) | 3010 |
| BpuEI | CTTGAG | 3 cut(s) | 1145, 2891, 3156 |
| BpuMI | CCSGG | 3 cut(s) | 55, 3029, 3635 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2124 |
| BsaI | GGTCTC | 1 cut(s) | 3264 |
| BsaJI | CCNNGG | 8 cut(s) | 69, 117, 253, 264, 511, 1143, 2856, 3028 |
| BsaWI | WCCGGW | 3 cut(s) | 1649, 2397, 2466 |
| Bse1I | ACTGG | 9 cut(s) | 160, 376, 1967, 2014, 2332, 2587, 2640, 2803, 3354 |
| Bse21I | CCTNAGG | 1 cut(s) | 3399 |
| Bse3DI | GCAATG | 3 cut(s) | 440, 2755, 2772 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2124 |
| BseAI | TCCGGA | 2 cut(s) | 2397, 2466 |
| BseBI | CCWGG | 3 cut(s) | 1088, 2599, 3235 |
| BseDI | CCNNGG | 8 cut(s) | 69, 117, 253, 264, 511, 1143, 2856, 3028 |
| BseGI | GGATG | 9 cut(s) | 724, 748, 1096, 1135, 2539, 2885, 3024, 3249, 3547 |
| BseJI | GATNNNNATC | 1 cut(s) | 2124 |
| BseMI | GCAATG | 3 cut(s) | 440, 2755, 2772 |
| BseMII | CTCAG | 6 cut(s) | 566, 966, 1389, 2985, 3249, 3665 |
| BseNI | ACTGG | 9 cut(s) | 160, 376, 1967, 2014, 2332, 2587, 2640, 2803, 3354 |
| BsePI | GCGCGC | 1 cut(s) | 3160 |
| BseRI | GAGGAG | 7 cut(s) | 1604, 1607, 2198, 2318, 2399, 3280, 3679 |
| BseSI | GKGCMC | 3 cut(s) | 392, 2780, 3119 |
| BseXI | GCAGC | 6 cut(s) | 196, 601, 943, 960, 2014, 3034 |
| BseYI | CCCAGC | 1 cut(s) | 1331 |
| Bsh1236I | CGCG | 2 cut(s) | 1995, 3162 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1999 |
| BshFI | GGCC | 6 cut(s) | 74, 339, 1870, 2054, 2597, 2778 |
| BsiEI | CGRYCG | 1 cut(s) | 1999 |
| BsiHKAI | GWGCWC | 1 cut(s) | 3119 |
| BsiSI | CCGG | 6 cut(s) | 55, 1650, 2398, 2467, 3028, 3634 |
| BslFI | GGGAC | 3 cut(s) | 37, 277, 2488 |
| BsmAI | GTCTC | 8 cut(s) | 161, 168, 1682, 1747, 1872, 2132, 3264, 3661 |
| BsmBI | CGTCTC | 2 cut(s) | 161, 1872 |
| BsmFI | GGGAC | 3 cut(s) | 37, 277, 2488 |
| BsmI | GAATGC | 1 cut(s) | 1278 |
| BsnI | GGCC | 6 cut(s) | 74, 339, 1870, 2054, 2597, 2778 |
| Bso31I | GGTCTC | 1 cut(s) | 3264 |
| Bsp119I | TTCGAA | 1 cut(s) | 3010 |
| Bsp120I | GGGCCC | 1 cut(s) | 2776 |
| Bsp1286I | GDGCHC | 3 cut(s) | 392, 2780, 3119 |
| Bsp13I | TCCGGA | 2 cut(s) | 2397, 2466 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 928 |
| Bsp19I | CCATGG | 3 cut(s) | 253, 511, 1143 |
| BspACI | CCGC | 9 cut(s) | 370, 420, 850, 1736, 2361, 2608, 2853, 3274, 3674 |
| BspANI | GGCC | 6 cut(s) | 74, 339, 1870, 2054, 2597, 2778 |
| BspCNI | CTCAG | 6 cut(s) | 567, 965, 1388, 2984, 3250, 3664 |
| BspEI | TCCGGA | 2 cut(s) | 2397, 2466 |
| BspFNI | CGCG | 2 cut(s) | 1995, 3162 |
| BspHI | TCATGA | 5 cut(s) | 661, 1519, 1684, 2497, 3463 |
| BspLI | GGNNCC | 7 cut(s) | 53, 67, 374, 2310, 2777, 2778, 3459 |
| BspMI | ACCTGC | 2 cut(s) | 881, 3025 |
| BspPI | GGATC | 4 cut(s) | 929, 1029, 1450, 2855 |
| BspQI | GCTCTTC | 2 cut(s) | 2214, 3436 |
| BspT104I | TTCGAA | 1 cut(s) | 3010 |
| BspTI | CTTAAG | 1 cut(s) | 2732 |
| BspTNI | GGTCTC | 1 cut(s) | 3264 |
| BsrDI | GCAATG | 3 cut(s) | 440, 2755, 2772 |
| BsrI | ACTGG | 9 cut(s) | 160, 376, 1967, 2014, 2332, 2587, 2640, 2803, 3354 |
| BssECI | CCNNGG | 8 cut(s) | 69, 117, 253, 264, 511, 1143, 2856, 3028 |
| BssHII | GCGCGC | 1 cut(s) | 3160 |
| BssSI | CACGAG | 1 cut(s) | 165 |
| BssT1I | CCWWGG | 6 cut(s) | 69, 117, 253, 264, 511, 1143 |
| Bst2BI | CACGAG | 1 cut(s) | 165 |
| Bst2UI | CCWGG | 3 cut(s) | 1088, 2599, 3235 |
| Bst4CI | ACNGT | 7 cut(s) | 142, 908, 1616, 1832, 1847, 3016, 3253 |
| Bst6I | CTCTTC | 5 cut(s) | 1651, 1936, 2175, 2214, 3436 |
| BstAFI | CTTAAG | 1 cut(s) | 2732 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 428, 2728 |
| BstBI | TTCGAA | 1 cut(s) | 3010 |
| BstC8I | GCNNGC | 6 cut(s) | 2170, 2192, 2205, 2595, 2729, 3162 |
| BstDEI | CTNAG | 9 cut(s) | 575, 928, 952, 1375, 2173, 2971, 3258, 3399, 3651 |
| BstDSI | CCRYGG | 4 cut(s) | 253, 511, 1143, 2856 |
| BstEII | GGTNACC | 2 cut(s) | 2918, 3065 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2435 |
| BstF5I | GGATG | 9 cut(s) | 724, 748, 1096, 1135, 2539, 2885, 3024, 3249, 3547 |
| BstFNI | CGCG | 2 cut(s) | 1995, 3162 |
| BstH2I | RGCGCY | 2 cut(s) | 805, 3607 |
| BstHHI | GCGC | 7 cut(s) | 804, 1995, 2190, 3162, 3164, 3531, 3606 |
| BstMAI | GTCTC | 8 cut(s) | 161, 168, 1682, 1747, 1872, 2132, 3264, 3661 |
| BstMCI | CGRYCG | 1 cut(s) | 1999 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 222, 428, 437, 2200, 2213, 2617, 2728, 3371, 3575 |
| BstNI | CCWGG | 3 cut(s) | 1088, 2599, 3235 |
| BstNSI | RCATGY | 6 cut(s) | 1511, 2327, 2593, 2731, 2755, 3674 |
| BstPI | GGTNACC | 2 cut(s) | 2918, 3065 |
| BstSCI | CCNGG | 6 cut(s) | 53, 1086, 2597, 3027, 3233, 3633 |
| BstSFI | CTRYAG | 1 cut(s) | 3252 |
| BstSLI | GKGCMC | 3 cut(s) | 392, 2780, 3119 |
| BstUI | CGCG | 2 cut(s) | 1995, 3162 |
| BstV1I | GCAGC | 6 cut(s) | 196, 601, 943, 960, 2014, 3034 |
| BstV2I | GAAGAC | 3 cut(s) | 1148, 1653, 3371 |
| BstX2I | RGATCY | 4 cut(s) | 1021, 1255, 1442, 2974 |
| BstYI | RGATCY | 4 cut(s) | 1021, 1255, 1442, 2974 |
| Bsu36I | CCTNAGG | 1 cut(s) | 3399 |
| BsuI | GTATCC | 2 cut(s) | 2579, 3397 |
| BsuRI | GGCC | 6 cut(s) | 74, 339, 1870, 2054, 2597, 2778 |
| BtgI | CCRYGG | 4 cut(s) | 253, 511, 1143, 2856 |
| BtgZI | GCGATG | 1 cut(s) | 2708 |
| BtsCI | GGATG | 9 cut(s) | 724, 748, 1096, 1135, 2539, 2885, 3024, 3249, 3547 |
| BtsIMutI | CAGTG | 7 cut(s) | 913, 1852, 2007, 2580, 2647, 3025, 3347 |
| BveI | ACCTGC | 2 cut(s) | 881, 3025 |
| Cac8I | GCNNGC | 6 cut(s) | 2170, 2192, 2205, 2595, 2729, 3162 |
| CciI | TCATGA | 5 cut(s) | 661, 1519, 1684, 2497, 3463 |
| CfoI | GCGC | 7 cut(s) | 804, 1995, 2190, 3162, 3164, 3531, 3606 |
| Cfr13I | GGNCC | 6 cut(s) | 51, 257, 338, 373, 2776, 2777 |
| CseI | GACGC | 1 cut(s) | 143 |
| Csp6I | GTAC | 2 cut(s) | 2737, 3368 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2845, 2880 |
| CviQI | GTAC | 2 cut(s) | 2737, 3368 |
| DdeI | CTNAG | 9 cut(s) | 575, 928, 952, 1375, 2173, 2971, 3258, 3399, 3651 |
| DraI | TTTAAA | 2 cut(s) | 597, 3388 |
| Eam1104I | CTCTTC | 5 cut(s) | 1651, 1936, 2175, 2214, 3436 |
| EarI | CTCTTC | 5 cut(s) | 1651, 1936, 2175, 2214, 3436 |
| EciI | GGCGGA | 1 cut(s) | 1751 |
| Eco130I | CCWWGG | 6 cut(s) | 69, 117, 253, 264, 511, 1143 |
| Eco24I | GRGCYC | 1 cut(s) | 2780 |
| Eco31I | GGTCTC | 1 cut(s) | 3264 |
| Eco32I | GATATC | 3 cut(s) | 400, 781, 2126 |
| Eco47I | GGWCC | 3 cut(s) | 51, 257, 373 |
| Eco47III | AGCGCT | 1 cut(s) | 803 |
| Eco57I | CTGAAG | 2 cut(s) | 1647, 2399 |
| Eco81I | CCTNAGG | 1 cut(s) | 3399 |
| Eco91I | GGTNACC | 2 cut(s) | 2918, 3065 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2435 |
| EcoO109I | RGGNCCY | 3 cut(s) | 51, 2776, 2777 |
| EcoO65I | GGTNACC | 2 cut(s) | 2918, 3065 |
| EcoRII | CCWGG | 3 cut(s) | 1086, 2597, 3233 |
| EcoRV | GATATC | 3 cut(s) | 400, 781, 2126 |
| EcoT14I | CCWWGG | 6 cut(s) | 69, 117, 253, 264, 511, 1143 |
| EcoT22I | ATGCAT | 3 cut(s) | 1280, 2757, 2887 |
| EcoT38I | GRGCYC | 1 cut(s) | 2780 |
| ErhI | CCWWGG | 6 cut(s) | 69, 117, 253, 264, 511, 1143 |
| Esp3I | CGTCTC | 2 cut(s) | 161, 1872 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1107, 1139, 3363, 3395 |
| FaqI | GGGAC | 3 cut(s) | 37, 277, 2488 |
| FauI | CCCGC | 2 cut(s) | 363, 843 |
| FauNDI | CATATG | 1 cut(s) | 2281 |
| FbaI | TGATCA | 2 cut(s) | 285, 1063 |
| Fnu4HI | GCNGC | 9 cut(s) | 185, 420, 590, 957, 974, 2028, 2362, 2609, 3048 |
| FokI | GGATG | 9 cut(s) | 711, 735, 1103, 1142, 2526, 2872, 3011, 3256, 3554 |
| FriOI | GRGCYC | 1 cut(s) | 2780 |
| Fsp4HI | GCNGC | 9 cut(s) | 185, 420, 590, 957, 974, 2028, 2362, 2609, 3048 |
| FspBI | CTAG | 3 cut(s) | 761, 1437, 2912 |
| GlaI | GCGC | 7 cut(s) | 803, 1994, 2189, 3161, 3163, 3530, 3605 |
| GluI | GCNGC | 9 cut(s) | 185, 420, 590, 957, 974, 2028, 2362, 2609, 3048 |
| GsaI | CCCAGC | 1 cut(s) | 1335 |
| GsuI | CTGGAG | 2 cut(s) | 143, 2727 |
| HaeII | RGCGCY | 2 cut(s) | 805, 3607 |
| HaeIII | GGCC | 6 cut(s) | 74, 339, 1870, 2054, 2597, 2778 |
| HapII | CCGG | 6 cut(s) | 55, 1650, 2398, 2467, 3028, 3634 |
| HgaI | GACGC | 1 cut(s) | 143 |
| HhaI | GCGC | 7 cut(s) | 804, 1995, 2190, 3162, 3164, 3531, 3606 |
| Hin6I | GCGC | 7 cut(s) | 802, 1993, 2188, 3160, 3162, 3529, 3604 |
| HinP1I | GCGC | 7 cut(s) | 802, 1993, 2188, 3160, 3162, 3529, 3604 |
| HincII | GTYRAC | 3 cut(s) | 1483, 2371, 3295 |
| HindII | GTYRAC | 3 cut(s) | 1483, 2371, 3295 |
| HindIII | AAGCTT | 2 cut(s) | 1976, 2201 |
| HpaI | GTTAAC | 2 cut(s) | 1483, 3295 |
| HpaII | CCGG | 6 cut(s) | 55, 1650, 2398, 2467, 3028, 3634 |
| Hpy166II | GTNNAC | 8 cut(s) | 558, 1483, 2371, 2739, 2967, 3089, 3117, 3295 |
| Hpy8I | GTNNAC | 8 cut(s) | 558, 1483, 2371, 2739, 2967, 3089, 3117, 3295 |
| Hpy99I | CGWCG | 3 cut(s) | 176, 192, 3624 |
| HpyAV | CCTTC | 9 cut(s) | 961, 1495, 1521, 2374, 2492, 2712, 2990, 3076, 3095 |
| HpyCH4III | ACNGT | 7 cut(s) | 142, 908, 1616, 1832, 1847, 3016, 3253 |
| HpyCH4IV | ACGT | 1 cut(s) | 679 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 222, 428, 437, 2200, 2213, 2617, 2728, 3371, 3575 |
| HpyF3I | CTNAG | 9 cut(s) | 575, 928, 952, 1375, 2173, 2971, 3258, 3399, 3651 |
| HpySE526I | ACGT | 1 cut(s) | 679 |
| HspAI | GCGC | 7 cut(s) | 802, 1993, 2188, 3160, 3162, 3529, 3604 |
| Kpn2I | TCCGGA | 2 cut(s) | 2397, 2466 |
| Ksp22I | TGATCA | 2 cut(s) | 285, 1063 |
| KspAI | GTTAAC | 2 cut(s) | 1483, 3295 |
| LguI | GCTCTTC | 2 cut(s) | 2214, 3436 |
| LmnI | GCTCC | 6 cut(s) | 28, 221, 629, 1192, 2185, 3457 |
| Lsp1109I | GCAGC | 6 cut(s) | 196, 601, 943, 960, 2014, 3034 |
| MaeI | CTAG | 3 cut(s) | 761, 1437, 2912 |
| MaeII | ACGT | 1 cut(s) | 679 |
| MfeI | CAATTG | 1 cut(s) | 3318 |
| MflI | RGATCY | 4 cut(s) | 1021, 1255, 1442, 2974 |
| MhlI | GDGCHC | 3 cut(s) | 392, 2780, 3119 |
| MlyI | GAGTC | 6 cut(s) | 107, 1604, 1667, 1736, 1922, 3163 |
| MmeI | TCCRAC | 4 cut(s) | 227, 1170, 2469, 2533 |
| Mph1103I | ATGCAT | 3 cut(s) | 1280, 2757, 2887 |
| MroI | TCCGGA | 2 cut(s) | 2397, 2466 |
| MslI | CAYNNNNRTG | 3 cut(s) | 753, 1283, 2133 |
| MspA1I | CMGCKG | 1 cut(s) | 956 |
| MspCI | CTTAAG | 1 cut(s) | 2732 |
| MspI | CCGG | 6 cut(s) | 55, 1650, 2398, 2467, 3028, 3634 |
| MspR9I | CCNGG | 6 cut(s) | 55, 1088, 2599, 3029, 3235, 3635 |
| MunI | CAATTG | 1 cut(s) | 3318 |
| Mva1269I | GAATGC | 1 cut(s) | 1278 |
| MvaI | CCWGG | 3 cut(s) | 1088, 2599, 3235 |
| MvnI | CGCG | 2 cut(s) | 1995, 3162 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 222, 428, 437, 2200, 2213, 2617, 2728, 3371, 3575 |
| NciI | CCSGG | 3 cut(s) | 55, 3029, 3635 |
| NcoI | CCATGG | 3 cut(s) | 253, 511, 1143 |
| NdeI | CATATG | 1 cut(s) | 2281 |
| NlaIV | GGNNCC | 7 cut(s) | 53, 67, 374, 2310, 2777, 2778, 3459 |
| NmuCI | GTSAC | 9 cut(s) | 33, 829, 1138, 2005, 2084, 2260, 2642, 3143, 3229 |
| NsiI | ATGCAT | 3 cut(s) | 1280, 2757, 2887 |
| NspI | RCATGY | 6 cut(s) | 1511, 2327, 2593, 2731, 2755, 3674 |
| NspV | TTCGAA | 1 cut(s) | 3010 |
| PaeI | GCATGC | 1 cut(s) | 2731 |
| PagI | TCATGA | 5 cut(s) | 661, 1519, 1684, 2497, 3463 |
| PaqCI | CACCTGC | 2 cut(s) | 881, 3025 |
| PauI | GCGCGC | 1 cut(s) | 3160 |
| PciI | ACATGT | 2 cut(s) | 1507, 2323 |
| PciSI | GCTCTTC | 2 cut(s) | 2214, 3436 |
| PctI | GAATGC | 1 cut(s) | 1278 |
| PfeI | GAWTC | 9 cut(s) | 685, 880, 1235, 1265, 1379, 1433, 1583, 3302, 3355 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 382 |
| PkrI | GCNGC | 9 cut(s) | 186, 421, 591, 958, 975, 2029, 2363, 2610, 3049 |
| Ple19I | CGATCG | 1 cut(s) | 1999 |
| PleI | GAGTC | 6 cut(s) | 106, 1603, 1666, 1735, 1922, 3163 |
| PpsI | GAGTC | 6 cut(s) | 106, 1603, 1666, 1735, 1922, 3163 |
| PpuMI | RGGWCCY | 1 cut(s) | 51 |
| PscI | ACATGT | 2 cut(s) | 1507, 2323 |
| PsiI | TTATAA | 2 cut(s) | 2954, 3195 |
| Psp5II | RGGWCCY | 1 cut(s) | 51 |
| Psp6I | CCWGG | 3 cut(s) | 1086, 2597, 3233 |
| PspEI | GGTNACC | 2 cut(s) | 2918, 3065 |
| PspFI | CCCAGC | 1 cut(s) | 1331 |
| PspGI | CCWGG | 3 cut(s) | 1086, 2597, 3233 |
| PspN4I | GGNNCC | 7 cut(s) | 53, 67, 374, 2310, 2777, 2778, 3459 |
| PspOMI | GGGCCC | 1 cut(s) | 2776 |
| PspPI | GGNCC | 6 cut(s) | 51, 257, 338, 373, 2776, 2777 |
| PspPPI | RGGWCCY | 1 cut(s) | 51 |
| PsuI | RGATCY | 4 cut(s) | 1021, 1255, 1442, 2974 |
| PteI | GCGCGC | 1 cut(s) | 3160 |
| PvuI | CGATCG | 1 cut(s) | 1999 |
| PvuII | CAGCTG | 1 cut(s) | 956 |
| RgaI | GCGATCGC | 1 cut(s) | 1999 |
| RsaI | GTAC | 2 cut(s) | 2738, 3369 |
| RsaNI | GTAC | 2 cut(s) | 2737, 3368 |
| RseI | CAYNNNNRTG | 3 cut(s) | 753, 1283, 2133 |
| SapI | GCTCTTC | 2 cut(s) | 2214, 3436 |
| SatI | GCNGC | 9 cut(s) | 185, 420, 590, 957, 974, 2028, 2362, 2609, 3048 |
| Sau96I | GGNCC | 6 cut(s) | 51, 257, 338, 373, 2776, 2777 |
| SchI | GAGTC | 6 cut(s) | 107, 1604, 1667, 1736, 1922, 3163 |
| ScrFI | CCNGG | 6 cut(s) | 55, 1088, 2599, 3029, 3235, 3635 |
| SduI | GDGCHC | 3 cut(s) | 392, 2780, 3119 |
| SfaAI | GCGATCGC | 1 cut(s) | 1999 |
| SfcI | CTRYAG | 1 cut(s) | 3252 |
| SfuI | TTCGAA | 1 cut(s) | 3010 |
| SgfI | GCGATCGC | 1 cut(s) | 1999 |
| SinI | GGWCC | 3 cut(s) | 51, 257, 373 |
| SmiI | ATTTAAAT | 1 cut(s) | 3388 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 753, 1283, 2133 |
| SmlI | CTYRAG | 4 cut(s) | 1124, 2732, 2870, 3171 |
| SmoI | CTYRAG | 4 cut(s) | 1124, 2732, 2870, 3171 |
| SpeI | ACTAGT | 1 cut(s) | 2911 |
| SphI | GCATGC | 1 cut(s) | 2731 |
| SsiI | CCGC | 9 cut(s) | 370, 420, 850, 1736, 2361, 2608, 2853, 3274, 3674 |
| SspI | AATATT | 3 cut(s) | 2271, 2525, 3280 |
| SspMI | CTAG | 3 cut(s) | 761, 1437, 2912 |
| StyD4I | CCNGG | 6 cut(s) | 53, 1086, 2597, 3027, 3233, 3633 |
| StyI | CCWWGG | 6 cut(s) | 69, 117, 253, 264, 511, 1143 |
| SwaI | ATTTAAAT | 1 cut(s) | 3388 |
| TaaI | ACNGT | 7 cut(s) | 142, 908, 1616, 1832, 1847, 3016, 3253 |
| TaiI | ACGT | 1 cut(s) | 682 |
| TaqI | TCGA | 8 cut(s) | 101, 174, 1751, 2347, 2457, 2825, 2989, 3010 |
| TatI | WGTACW | 2 cut(s) | 2736, 3367 |
| TauI | GCSGC | 3 cut(s) | 422, 2364, 2611 |
| TfiI | GAWTC | 9 cut(s) | 685, 880, 1235, 1265, 1379, 1433, 1583, 3302, 3355 |
| TscAI | CASTG | 7 cut(s) | 913, 1852, 2014, 2587, 2647, 3025, 3354 |
| TseFI | GTSAC | 9 cut(s) | 33, 829, 1138, 2005, 2084, 2260, 2642, 3143, 3229 |
| TseI | GCWGC | 6 cut(s) | 184, 589, 956, 973, 2027, 3047 |
| Tsp45I | GTSAC | 9 cut(s) | 33, 829, 1138, 2005, 2084, 2260, 2642, 3143, 3229 |
| TspGWI | ACGGA | 4 cut(s) | 471, 1756, 1896, 2730 |
| TspRI | CASTG | 7 cut(s) | 913, 1852, 2014, 2587, 2647, 3025, 3354 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 382 |
| Vha464I | CTTAAG | 1 cut(s) | 2732 |
| VneI | GTGCAC | 1 cut(s) | 3115 |
| VpaK11BI | GGWCC | 3 cut(s) | 51, 257, 373 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2435 |
| XapI | RAATTY | 6 cut(s) | 425, 1680, 1937, 2147, 2818, 3474 |
| XceI | RCATGY | 6 cut(s) | 1511, 2327, 2593, 2731, 2755, 3674 |
| XspI | CTAG | 3 cut(s) | 761, 1437, 2912 |
| Zsp2I | ATGCAT | 3 cut(s) | 1280, 2757, 2887 |