RchiOBHm_Chr7g0236221

Golgi vesicle prefusion complex stabilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001047
Physical Location & Seq
Forward (+)
61063161 .. 61063879
719 bp
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UTR
Exon/CDS
Intron
PRQ21167

Sequence Viewer

Length: 459 bp
ATGCTATGGTTAGTCAAACTGATGGGTCTTGGTGGCATAGTGTTTGATTTAGTCAAGCTTTTAGGTAACCTAGAGCAGCTAATTAAGGTTCACTCTGAGCCGCCCACCCGCACTATGGTAATTGGGAACAATCAAGTAATGTCATACACTTGTTCTCACAATTCTTCCGCCGATGACCTCAACAAACATGTCATGAAGGTTTTCAAGGATGCGACCAAAGATTGTGAGGACAAGGTCTTGGAAGAAATGTTTGACGCCATTGGGGACTACGACTCCCTTAACGAATATGCCTTTCATGTCTTCAGTATATTGGCAGATTCCGGCATGCCTGACGAAGCCAAAGAGCTCTTTAAACCTCGCTCTGAGTTGGCTGTACTGCCTGATGTGGCCATCTTCACCATTGTCATTCGGGCCTATGCCAATGCCGGCAAGATCAACGCTGCTCACCAGGTCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

152

Amino Acids

16.95

Weight (kDa)

5.11

Isoelectric Point (pI)

30.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 453
AciI CCGC 3 cut(s) 101, 109, 168
AcoI YGGCCR 1 cut(s) 387
AcuI CTGAAG 1 cut(s) 286
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 1 cut(s) 375
AfiI CCNNNNNNNGG 1 cut(s) 115
AflIII ACRYGT 1 cut(s) 187
AgsI TTSAA 1 cut(s) 205
AjnI CCWGG 1 cut(s) 447
AluBI AGCT 3 cut(s) 58, 79, 346
AluI AGCT 3 cut(s) 58, 79, 346
Alw21I GWGCWC 1 cut(s) 348
AoxI GGCC 2 cut(s) 387, 411
ApeKI GCWGC 2 cut(s) 76, 440
Asp700I GAANNNNTTC 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 411
AsuHPI GGTGA 2 cut(s) 388, 437
BalI TGGCCA 1 cut(s) 389
BanII GRGCYC 1 cut(s) 348
BbsI GAAGAC 1 cut(s) 292
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 2 cut(s) 88, 427
BccI CCATC 2 cut(s) 16, 398
BciT130I CCWGG 1 cut(s) 449
BfaI CTAG 2 cut(s) 71, 457
BisI GCNGC 3 cut(s) 77, 101, 441
BlsI GCNGC 3 cut(s) 78, 102, 442
Bme1390I CCNGG 1 cut(s) 449
BmgT120I GGNCC 1 cut(s) 411
BmrFI CCNGG 1 cut(s) 449
BmsI GCATC 1 cut(s) 199
BpiI GAAGAC 1 cut(s) 292
BsaHI GRCGYC 1 cut(s) 255
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 1 cut(s) 115
Bse118I RCCGGY 1 cut(s) 425
BseBI CCWGG 1 cut(s) 449
BseGI GGATG 1 cut(s) 214
BseLI CCNNNNNNNGG 1 cut(s) 115
BseMII CTCAG 2 cut(s) 87, 354
BseXI GCAGC 2 cut(s) 88, 427
BshFI GGCC 2 cut(s) 389, 413
BsiHKAI GWGCWC 1 cut(s) 348
BsiSI CCGG 2 cut(s) 321, 426
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 1 cut(s) 115
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 2 cut(s) 389, 413
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 1 cut(s) 432
BspACI CCGC 3 cut(s) 101, 109, 168
BspANI GGCC 2 cut(s) 389, 413
BspCNI CTCAG 2 cut(s) 88, 355
BspHI TCATGA 1 cut(s) 192
BsrFI RCCGGY 1 cut(s) 425
BssAI RCCGGY 1 cut(s) 425
BssMI GATC 1 cut(s) 432
BssNI GRCGYC 1 cut(s) 255
Bst2UI CCWGG 1 cut(s) 449
BstACI GRCGYC 1 cut(s) 255
BstC8I GCNNGC 2 cut(s) 326, 427
BstDEI CTNAG 2 cut(s) 96, 363
BstEII GGTNACC 1 cut(s) 65
BstF5I GGATG 1 cut(s) 214
BstKTI GATC 1 cut(s) 435
BstMBI GATC 1 cut(s) 432
BstNI CCWGG 1 cut(s) 449
BstNSI RCATGY 2 cut(s) 191, 328
BstPI GGTNACC 1 cut(s) 65
BstSCI CCNGG 1 cut(s) 447
BstV1I GCAGC 2 cut(s) 88, 427
BstV2I GAAGAC 1 cut(s) 292
BsuRI GGCC 2 cut(s) 389, 413
BtsCI GGATG 1 cut(s) 214
Cac8I GCNNGC 2 cut(s) 326, 427
CciI TCATGA 1 cut(s) 192
Cfr10I RCCGGY 1 cut(s) 425
Cfr13I GGNCC 1 cut(s) 411
CseI GACGC 1 cut(s) 263
CsiI ACCWGGT 1 cut(s) 447
Csp6I GTAC 1 cut(s) 374
CviAII CATG 4 cut(s) 188, 193, 296, 325
CviJI RGCY 8 cut(s) 58, 79, 100, 338, 346, 371, 389, 413
CviKI_1 RGCY 8 cut(s) 58, 79, 100, 338, 346, 371, 389, 413
CviQI GTAC 1 cut(s) 374
DdeI CTNAG 2 cut(s) 96, 363
DpnI GATC 1 cut(s) 434
DpnII GATC 1 cut(s) 432
DraI TTTAAA 1 cut(s) 352
EaeI YGGCCR 1 cut(s) 387
EciI GGCGGA 1 cut(s) 157
Ecl136II GAGCTC 1 cut(s) 346
Eco24I GRGCYC 1 cut(s) 348
Eco53kI GAGCTC 1 cut(s) 346
Eco57I CTGAAG 1 cut(s) 286
Eco91I GGTNACC 1 cut(s) 65
EcoICRI GAGCTC 1 cut(s) 346
EcoO65I GGTNACC 1 cut(s) 65
EcoRII CCWGG 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 348
FaeI CATG 4 cut(s) 191, 196, 299, 328
FaqI GGGAC 1 cut(s) 278
FatI CATG 4 cut(s) 187, 192, 295, 324
FauI CCCGC 1 cut(s) 116
FblI GTMKAC 1 cut(s) 453
Fnu4HI GCNGC 3 cut(s) 77, 101, 441
FokI GGATG 1 cut(s) 221
FriOI GRGCYC 1 cut(s) 348
Fsp4HI GCNGC 3 cut(s) 77, 101, 441
FspBI CTAG 2 cut(s) 71, 457
GluI GCNGC 3 cut(s) 77, 101, 441
HaeIII GGCC 2 cut(s) 389, 413
HapII CCGG 2 cut(s) 321, 426
HgaI GACGC 1 cut(s) 263
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 4 cut(s) 191, 196, 299, 328
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 2 cut(s) 272, 317
HpaII CCGG 2 cut(s) 321, 426
HphI GGTGA 2 cut(s) 388, 437
Hpy166II GTNNAC 2 cut(s) 91, 454
Hpy188I TCNGA 2 cut(s) 97, 364
Hpy188III TCNNGA 1 cut(s) 193
Hpy8I GTNNAC 2 cut(s) 91, 454
HpyAV CCTTC 1 cut(s) 190
HpyF3I CTNAG 2 cut(s) 96, 363
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 4 cut(s) 191, 196, 299, 328
KroI GCCGGC 1 cut(s) 425
KroNI GCCGGC 1 cut(s) 427
Kzo9I GATC 1 cut(s) 432
LpnPI CCDG 5 cut(s) 334, 342, 393, 434, 439
Lsp1109I GCAGC 2 cut(s) 88, 427
LweI GCATC 1 cut(s) 199
MabI ACCWGGT 1 cut(s) 447
MaeI CTAG 2 cut(s) 71, 457
MaeIII GTNAC 1 cut(s) 65
MalI GATC 1 cut(s) 434
MboI GATC 1 cut(s) 432
MboII GAAGA 4 cut(s) 156, 254, 292, 385
MhlI GDGCHC 1 cut(s) 348
MlsI TGGCCA 1 cut(s) 389
MluCI AATT 3 cut(s) 81, 120, 160
MluNI TGGCCA 1 cut(s) 389
MlyI GAGTC 1 cut(s) 266
MnlI CCTC 3 cut(s) 188, 220, 366
Mox20I TGGCCA 1 cut(s) 389
MroNI GCCGGC 1 cut(s) 425
MroXI GAANNNNTTC 1 cut(s) 200
MscI TGGCCA 1 cut(s) 389
MseI TTAA 3 cut(s) 84, 279, 351
Msp20I TGGCCA 1 cut(s) 389
MspI CCGG 2 cut(s) 321, 426
MspR9I CCNGG 1 cut(s) 449
MvaI CCWGG 1 cut(s) 449
NaeI GCCGGC 1 cut(s) 427
NdeII GATC 1 cut(s) 432
NgoMIV GCCGGC 1 cut(s) 425
NlaIII CATG 4 cut(s) 191, 196, 299, 328
NspI RCATGY 2 cut(s) 191, 328
PaeI GCATGC 1 cut(s) 328
PagI TCATGA 1 cut(s) 192
PciI ACATGT 1 cut(s) 187
PdiI GCCGGC 1 cut(s) 427
PdmI GAANNNNTTC 1 cut(s) 200
PfeI GAWTC 1 cut(s) 317
PflFI GACNNNGTC 1 cut(s) 233
PkrI GCNGC 3 cut(s) 78, 102, 442
PleI GAGTC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 266
PscI ACATGT 1 cut(s) 187
Psp124BI GAGCTC 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 447
PspEI GGTNACC 1 cut(s) 65
PspGI CCWGG 1 cut(s) 447
PspPI GGNCC 1 cut(s) 411
PsyI GACNNNGTC 1 cut(s) 233
RsaI GTAC 1 cut(s) 375
RsaNI GTAC 1 cut(s) 374
SacI GAGCTC 1 cut(s) 348
SaqAI TTAA 3 cut(s) 84, 279, 351
SatI GCNGC 3 cut(s) 77, 101, 441
Sau3AI GATC 1 cut(s) 432
Sau96I GGNCC 1 cut(s) 411
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 1 cut(s) 449
SduI GDGCHC 1 cut(s) 348
SexAI ACCWGGT 1 cut(s) 447
SfaNI GCATC 1 cut(s) 199
SphI GCATGC 1 cut(s) 328
Sse9I AATT 3 cut(s) 81, 120, 160
SsiI CCGC 3 cut(s) 101, 109, 168
SspMI CTAG 2 cut(s) 71, 457
SstI GAGCTC 1 cut(s) 348
StyD4I CCNGG 1 cut(s) 447
TasI AATT 3 cut(s) 81, 120, 160
TatI WGTACW 1 cut(s) 373
TauI GCSGC 1 cut(s) 103
TfiI GAWTC 1 cut(s) 317
Tru1I TTAA 3 cut(s) 84, 279, 351
Tru9I TTAA 3 cut(s) 84, 279, 351
TseI GCWGC 2 cut(s) 76, 440
TspDTI ATGAA 2 cut(s) 209, 284
Tth111I GACNNNGTC 1 cut(s) 233
XceI RCATGY 2 cut(s) 191, 328
XcmI CCANNNNNNNNNTGG 1 cut(s) 112
XmiI GTMKAC 1 cut(s) 453
XmnI GAANNNNTTC 1 cut(s) 200
XspI CTAG 2 cut(s) 71, 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.