Rroxscaffold_3G00227720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001047
Physical Location & Seq
Forward (+)
11948037 .. 11948750
714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00227720.1

Sequence Viewer

Length: 543 bp
ATGACCGATCGGAGGCCGAGCTCTAACGGCGTGGATCTAGTTTGGACCGAGTTGGATGGGCGAGTCCGGATTTTGGATCGGGGTCGTCGGATTTTCGACGGTGATAGGGATTGTACAATCGCTTTGGTTGGCCCCGGTGGTTCGCTGCGTGTCGTGGAATGGGGGCGGATTTGGGGGTCCTTCGATCCCGGAGATGGTATAGTATCGTCGGCGGTGAACCAAGGTGACGGCGGCTATGGGGATGGCGGTTTCCGGGCGATGGAGCTCCTACTTGGGCTCTATGGGCTGGTGATCATTCCGGGGATGGGAATGCTCAACGTGGTGGCTTTTTTGGCTATGCTTGGTGATGGCCTGCAGGTGGTCGGCAGGGGAGGCGGCGTCTTTGCCGGCGACGGCGACATGCAACTTGGGAGTGGCCATGTTGTGGAGCAGTGGTTGTTAGGGTTTCGAAAAATTTGGGTCTGTTGGGCTCAAGTATTGGACTTAGTCCGGATTGGGATGCATTGGGGCCATATGGGCTCGAGGTTTTATTTTTTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

180

Amino Acids

19.55

Weight (kDa)

5.52

Isoelectric Point (pI)

26.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 346
Acc36I ACCTGC 1 cut(s) 346
AccB7I CCANNNNNTGG 1 cut(s) 424
AccIII TCCGGA 2 cut(s) 66, 489
AciI CCGC 5 cut(s) 166, 212, 231, 246, 375
AclWI GGATC 3 cut(s) 42, 84, 179
AcoI YGGCCR 1 cut(s) 415
AcsI RAATTY 1 cut(s) 453
AcyI GRCGYC 1 cut(s) 378
AfaI GTAC 1 cut(s) 115
AfiI CCNNNNNNNGG 7 cut(s) 12, 73, 194, 259, 305, 358, 424
AluBI AGCT 2 cut(s) 21, 265
AluI AGCT 2 cut(s) 21, 265
Alw21I GWGCWC 2 cut(s) 23, 267
AlwI GGATC 3 cut(s) 42, 84, 179
Ama87I CYCGRG 1 cut(s) 520
Aor13HI TCCGGA 2 cut(s) 66, 489
AoxI GGCC 5 cut(s) 14, 130, 349, 415, 508
ApeKI GCWGC 1 cut(s) 145
ApoI RAATTY 1 cut(s) 453
AspS9I GGNCC 4 cut(s) 45, 131, 177, 508
AsuC2I CCSGG 4 cut(s) 135, 189, 254, 300
AsuHPI GGTGA 5 cut(s) 113, 226, 236, 301, 356
AsuII TTCGAA 1 cut(s) 448
AvaI CYCGRG 1 cut(s) 520
AvaII GGWCC 2 cut(s) 45, 177
BalI TGGCCA 1 cut(s) 417
BanII GRGCYC 5 cut(s) 23, 267, 279, 472, 521
Bbv12I GWGCWC 2 cut(s) 23, 267
BbvI GCAGC 1 cut(s) 132
BccI CCATC 6 cut(s) 50, 188, 236, 253, 298, 341
BceAI ACGGC 3 cut(s) 43, 244, 409
BclI TGATCA 1 cut(s) 291
BcnI CCSGG 4 cut(s) 135, 189, 254, 300
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 1 cut(s) 353
BfuAI ACCTGC 1 cut(s) 346
BglI GCCNNNNNGGC 1 cut(s) 516
BisI GCNGC 3 cut(s) 146, 232, 376
BlsI GCNGC 3 cut(s) 147, 233, 377
Bme1390I CCNGG 4 cut(s) 135, 189, 254, 300
Bme18I GGWCC 2 cut(s) 45, 177
BmeT110I CYCGRG 1 cut(s) 520
BmgT120I GGNCC 4 cut(s) 45, 131, 177, 508
BmiI GGNNCC 3 cut(s) 133, 178, 509
BmrFI CCNGG 4 cut(s) 135, 189, 254, 300
BmsI GCATC 1 cut(s) 489
Bpu14I TTCGAA 1 cut(s) 448
BpuEI CTTGAG 1 cut(s) 456
BpuMI CCSGG 4 cut(s) 135, 189, 254, 300
BsaHI GRCGYC 1 cut(s) 378
BsaJI CCNNGG 3 cut(s) 133, 220, 299
BsaWI WCCGGW 2 cut(s) 66, 489
BsaXI ACNNNNNCTCC 4 cut(s) 363, 393, 419, 449
Bsc4I CCNNNNNNNGG 7 cut(s) 12, 73, 194, 259, 305, 358, 424
Bse118I RCCGGY 1 cut(s) 386
BseAI TCCGGA 2 cut(s) 66, 489
BseDI CCNNGG 3 cut(s) 133, 220, 299
BseGI GGATG 4 cut(s) 61, 247, 309, 504
BseLI CCNNNNNNNGG 7 cut(s) 12, 73, 194, 259, 305, 358, 424
BseXI GCAGC 1 cut(s) 132
Bsh1285I CGRYCG 1 cut(s) 10
BshFI GGCC 5 cut(s) 16, 132, 351, 417, 510
BsiEI CGRYCG 1 cut(s) 10
BsiHKAI GWGCWC 2 cut(s) 23, 267
BsiHKCI CYCGRG 1 cut(s) 520
BsiSI CCGG 7 cut(s) 67, 135, 189, 253, 299, 387, 490
BslI CCNNNNNNNGG 7 cut(s) 12, 73, 194, 259, 305, 358, 424
BsmI GAATGC 1 cut(s) 315
BsnI GGCC 5 cut(s) 16, 132, 351, 417, 510
BsoBI CYCGRG 1 cut(s) 520
Bsp119I TTCGAA 1 cut(s) 448
Bsp1286I GDGCHC 5 cut(s) 23, 267, 279, 472, 521
Bsp13I TCCGGA 2 cut(s) 66, 489
Bsp1407I TGTACA 1 cut(s) 113
Bsp143I GATC 5 cut(s) 7, 34, 76, 184, 291
BspACI CCGC 5 cut(s) 166, 212, 231, 246, 375
BspANI GGCC 5 cut(s) 16, 132, 351, 417, 510
BspEI TCCGGA 2 cut(s) 66, 489
BspLI GGNNCC 3 cut(s) 133, 178, 509
BspMAI CTGCAG 1 cut(s) 357
BspMI ACCTGC 1 cut(s) 346
BspPI GGATC 3 cut(s) 42, 84, 179
BspT104I TTCGAA 1 cut(s) 448
BsrFI RCCGGY 1 cut(s) 386
BsrGI TGTACA 1 cut(s) 113
BssAI RCCGGY 1 cut(s) 386
BssECI CCNNGG 3 cut(s) 133, 220, 299
BssMI GATC 5 cut(s) 7, 34, 76, 184, 291
BssNI GRCGYC 1 cut(s) 378
BssT1I CCWWGG 1 cut(s) 220
Bst4CI ACNGT 1 cut(s) 101
BstACI GRCGYC 1 cut(s) 378
BstAUI TGTACA 1 cut(s) 113
BstBI TTCGAA 1 cut(s) 448
BstC8I GCNNGC 2 cut(s) 353, 388
BstDEI CTNAG 1 cut(s) 484
BstF5I GGATG 4 cut(s) 61, 247, 309, 504
BstKTI GATC 5 cut(s) 10, 37, 79, 187, 294
BstMBI GATC 5 cut(s) 7, 34, 76, 184, 291
BstMCI CGRYCG 1 cut(s) 10
BstMWI GCNNNNNNNGC 5 cut(s) 27, 283, 332, 372, 516
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 4 cut(s) 133, 187, 252, 298
BstSFI CTRYAG 1 cut(s) 353
BstV1I GCAGC 1 cut(s) 132
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BsuRI GGCC 5 cut(s) 16, 132, 351, 417, 510
BtgZI GCGATG 1 cut(s) 272
BtsCI GGATG 4 cut(s) 61, 247, 309, 504
BtsI GCAGTG 1 cut(s) 437
BtsIMutI CAGTG 1 cut(s) 437
BveI ACCTGC 1 cut(s) 346
Cac8I GCNNGC 2 cut(s) 353, 388
Cfr10I RCCGGY 1 cut(s) 386
Cfr13I GGNCC 4 cut(s) 45, 131, 177, 508
CseI GACGC 1 cut(s) 367
Csp6I GTAC 1 cut(s) 114
CviAII CATG 2 cut(s) 400, 419
CviQI GTAC 1 cut(s) 114
DdeI CTNAG 1 cut(s) 484
DpnI GATC 5 cut(s) 9, 36, 78, 186, 293
DpnII GATC 5 cut(s) 7, 34, 76, 184, 291
EaeI YGGCCR 1 cut(s) 415
EciI GGCGGA 1 cut(s) 181
Ecl136II GAGCTC 2 cut(s) 21, 265
Eco130I CCWWGG 1 cut(s) 220
Eco24I GRGCYC 5 cut(s) 23, 267, 279, 472, 521
Eco47I GGWCC 2 cut(s) 45, 177
Eco53kI GAGCTC 2 cut(s) 21, 265
Eco88I CYCGRG 1 cut(s) 520
EcoICRI GAGCTC 2 cut(s) 21, 265
EcoO109I RGGNCCY 1 cut(s) 177
EcoT14I CCWWGG 1 cut(s) 220
EcoT22I ATGCAT 1 cut(s) 504
EcoT38I GRGCYC 5 cut(s) 23, 267, 279, 472, 521
ErhI CCWWGG 1 cut(s) 220
FaeI CATG 2 cut(s) 403, 422
FaiI YATR 8 cut(s) 200, 237, 282, 338, 401, 420, 513, 515
FatI CATG 2 cut(s) 399, 418
FauNDI CATATG 1 cut(s) 513
FbaI TGATCA 1 cut(s) 291
Fnu4HI GCNGC 3 cut(s) 146, 232, 376
FokI GGATG 4 cut(s) 68, 254, 316, 511
FriOI GRGCYC 5 cut(s) 23, 267, 279, 472, 521
Fsp4HI GCNGC 3 cut(s) 146, 232, 376
FspBI CTAG 1 cut(s) 38
GluI GCNGC 3 cut(s) 146, 232, 376
HaeIII GGCC 5 cut(s) 16, 132, 351, 417, 510
HapII CCGG 7 cut(s) 67, 135, 189, 253, 299, 387, 490
HgaI GACGC 1 cut(s) 367
Hin1I GRCGYC 1 cut(s) 378
Hin1II CATG 2 cut(s) 403, 422
HinfI GANTC 1 cut(s) 63
HpaII CCGG 7 cut(s) 67, 135, 189, 253, 299, 387, 490
HphI GGTGA 5 cut(s) 113, 226, 236, 301, 356
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 2 cut(s) 12, 90
Hpy188III TCNNGA 2 cut(s) 67, 490
Hpy8I GTNNAC 1 cut(s) 217
Hpy99I CGWCG 4 cut(s) 90, 101, 211, 395
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 3 cut(s) 355, 403, 502
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 283, 332, 372, 516
HpyF3I CTNAG 1 cut(s) 484
HpySE526I ACGT 1 cut(s) 318
Hsp92I GRCGYC 1 cut(s) 378
Hsp92II CATG 2 cut(s) 403, 422
Kpn2I TCCGGA 2 cut(s) 66, 489
KroI GCCGGC 1 cut(s) 386
KroNI GCCGGC 1 cut(s) 388
Ksp22I TGATCA 1 cut(s) 291
Kzo9I GATC 5 cut(s) 7, 34, 76, 184, 291
LmnI GCTCC 3 cut(s) 262, 270, 427
Lsp1109I GCAGC 1 cut(s) 132
LweI GCATC 1 cut(s) 489
MaeI CTAG 1 cut(s) 38
MaeII ACGT 1 cut(s) 318
MaeIII GTNAC 1 cut(s) 224
MalI GATC 5 cut(s) 9, 36, 78, 186, 293
MboI GATC 5 cut(s) 7, 34, 76, 184, 291
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 5 cut(s) 23, 267, 279, 472, 521
MlsI TGGCCA 1 cut(s) 417
MluCI AATT 1 cut(s) 453
MluNI TGGCCA 1 cut(s) 417
MlyI GAGTC 1 cut(s) 72
MmeI TCCRAC 2 cut(s) 33, 68
MnlI CCTC 3 cut(s) 6, 365, 516
Mox20I TGGCCA 1 cut(s) 417
Mph1103I ATGCAT 1 cut(s) 504
MroI TCCGGA 2 cut(s) 66, 489
MroNI GCCGGC 1 cut(s) 386
MscI TGGCCA 1 cut(s) 417
MseI TTAA 1 cut(s) 541
Msp20I TGGCCA 1 cut(s) 417
MspI CCGG 7 cut(s) 67, 135, 189, 253, 299, 387, 490
MspR9I CCNGG 4 cut(s) 135, 189, 254, 300
Mva1269I GAATGC 1 cut(s) 315
MwoI GCNNNNNNNGC 5 cut(s) 27, 283, 332, 372, 516
NaeI GCCGGC 1 cut(s) 388
NciI CCSGG 4 cut(s) 135, 189, 254, 300
NdeI CATATG 1 cut(s) 513
NdeII GATC 5 cut(s) 7, 34, 76, 184, 291
NgoMIV GCCGGC 1 cut(s) 386
NlaIII CATG 2 cut(s) 403, 422
NlaIV GGNNCC 3 cut(s) 133, 178, 509
NmeAIII GCCGAG 1 cut(s) 42
NmuCI GTSAC 1 cut(s) 224
NsiI ATGCAT 1 cut(s) 504
NspI RCATGY 1 cut(s) 403
NspV TTCGAA 1 cut(s) 448
PaeR7I CTCGAG 1 cut(s) 520
PaqCI CACCTGC 1 cut(s) 346
PctI GAATGC 1 cut(s) 315
PdiI GCCGGC 1 cut(s) 388
PflFI GACNNNGTC 1 cut(s) 485
PflMI CCANNNNNTGG 1 cut(s) 424
PfoI TCCNGGA 1 cut(s) 187
PkrI GCNGC 3 cut(s) 147, 233, 377
Ple19I CGATCG 1 cut(s) 10
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
PpuMI RGGWCCY 1 cut(s) 177
Psp124BI GAGCTC 2 cut(s) 23, 267
Psp5II RGGWCCY 1 cut(s) 177
PspN4I GGNNCC 3 cut(s) 133, 178, 509
PspPI GGNCC 4 cut(s) 45, 131, 177, 508
PspPPI RGGWCCY 1 cut(s) 177
PspXI VCTCGAGB 1 cut(s) 520
PstI CTGCAG 1 cut(s) 357
PsuI RGATCY 1 cut(s) 34
PsyI GACNNNGTC 1 cut(s) 485
PvuI CGATCG 1 cut(s) 10
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
SacI GAGCTC 2 cut(s) 23, 267
SaqAI TTAA 1 cut(s) 541
SatI GCNGC 3 cut(s) 146, 232, 376
Sau3AI GATC 5 cut(s) 7, 34, 76, 184, 291
Sau96I GGNCC 4 cut(s) 45, 131, 177, 508
SbfI CCTGCAGG 1 cut(s) 357
SchI GAGTC 1 cut(s) 72
ScrFI CCNGG 4 cut(s) 135, 189, 254, 300
SdaI CCTGCAGG 1 cut(s) 357
SduI GDGCHC 5 cut(s) 23, 267, 279, 472, 521
SetI ASST 6 cut(s) 23, 226, 267, 321, 360, 527
SfaNI GCATC 1 cut(s) 489
SfcI CTRYAG 1 cut(s) 353
Sfr274I CTCGAG 1 cut(s) 520
SfuI TTCGAA 1 cut(s) 448
SinI GGWCC 2 cut(s) 45, 177
SlaI CTCGAG 1 cut(s) 520
SmlI CTYRAG 2 cut(s) 471, 520
SmoI CTYRAG 2 cut(s) 471, 520
Sse8387I CCTGCAGG 1 cut(s) 357
Sse9I AATT 1 cut(s) 453
SsiI CCGC 5 cut(s) 166, 212, 231, 246, 375
SspMI CTAG 1 cut(s) 38
SstI GAGCTC 2 cut(s) 23, 267
StyD4I CCNGG 4 cut(s) 133, 187, 252, 298
StyI CCWWGG 1 cut(s) 220
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 1 cut(s) 321
TaqI TCGA 4 cut(s) 96, 183, 448, 521
TaqII GACCGA 2 cut(s) 20, 62
TasI AATT 1 cut(s) 453
TatI WGTACW 1 cut(s) 113
TauI GCSGC 2 cut(s) 234, 378
Tru1I TTAA 1 cut(s) 541
Tru9I TTAA 1 cut(s) 541
TscAI CASTG 1 cut(s) 437
TseFI GTSAC 1 cut(s) 224
TseI GCWGC 1 cut(s) 145
Tsp45I GTSAC 1 cut(s) 224
TspRI CASTG 1 cut(s) 437
Tth111I GACNNNGTC 1 cut(s) 485
Van91I CCANNNNNTGG 1 cut(s) 424
VpaK11BI GGWCC 2 cut(s) 45, 177
XapI RAATTY 1 cut(s) 453
XceI RCATGY 1 cut(s) 403
XhoI CTCGAG 1 cut(s) 520
XspI CTAG 1 cut(s) 38
Zsp2I ATGCAT 1 cut(s) 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.