Rroxscaffold_3G00225330

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001047
Physical Location & Seq
Reverse (-)
8607515 .. 8608426
912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00225330.1

Sequence Viewer

Length: 912 bp
ATGGCGAGTACTAGTTCCCCCAATTCTTCTGTCCGTGTCATCAAGAAATATGTAGTGGATGGTCTTAAGGACAAGGCCCTGGACAAGGCCTTGGAAGAACACTTGAACATGATTCGGGACAAAGACTCCCTTCACAAATATGGGGCAAAGCTGTACAAATCTATAGCAAAATTCGCCATCGTTGATGAAGCCCTAGAGCAGCTAATTAAGGTTCACTCTGAGCCGCCCACCCGCACTATTGTCATTGGGAACAATCAAGTAATGTCATACTCTTATTCTCGCAACTCTTCTGCTGATGATCTCAAGGAACATGCAATGGAGGTTTTCAAGGACGCGACCAAAGATTGTGAGGACAAGGACATGAAAGAAAAATTTGACATGATTCAGGACTACGGTTCCCTTGAGAAATCTGCGTTTGTGTTCTTCAATTCTATGGTGAATTCTGGCTTCCTTGATGAAGCTGAAGAGCTCCTTAAGAAGGTCATACTGCCTGTTGTGGCGGTCCACACCTATGTCATTGGGGTTGGGGTCGCTGCCGGCAATACCAATGCTGCTCTCAAGGCCTTTCAGCACATGTCAGCCTCTGGGGTTGCTCCAAACTCCTATACTTATGCAGTTTTGATCAAGGGACTCACTGCTGACCCCAACTACTGTGGAGATGCCAAGAAGTTCTTGCTTGAGATGATGGACAAGGGTATGAGGCCCAATGCTGCTACCTACACTGCAGTGATAGAGGGCTTTGCAAAGCTGGAGGACAAGGCAGCTGAGGAGGAGGGCAAACAGTTTGTGGATGTGATGATGCATAAGGGGTTTGTGCCAAATGCAAAGGCTATGATGGAGGTTCTAAAGGGAAGGCCAAAACCTTTAATTAGAAGGGTTATCAACATTGCGCTTTCCAAGTTGAAGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

303

Amino Acids

33.49

Weight (kDa)

8.25

Isoelectric Point (pI)

26.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 172 - 211 4.1e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 335
AciI CCGC 3 cut(s) 224, 232, 500
AcsI RAATTY 3 cut(s) 170, 371, 439
AcuI CTGAAG 1 cut(s) 483
AfaI GTAC 2 cut(s) 10, 155
AfiI CCNNNNNNNGG 2 cut(s) 85, 478
AflII CTTAAG 2 cut(s) 65, 473
AflIII ACRYGT 1 cut(s) 573
AgsI TTSAA 4 cut(s) 106, 328, 427, 904
AhlI ACTAGT 1 cut(s) 11
AjnI CCWGG 1 cut(s) 78
AleI CACNNNNGTG 1 cut(s) 725
AluBI AGCT 6 cut(s) 151, 202, 461, 469, 748, 764
AluI AGCT 6 cut(s) 151, 202, 461, 469, 748, 764
Alw21I GWGCWC 1 cut(s) 471
AlwNI CAGNNNCTG 1 cut(s) 584
AoxI GGCC 5 cut(s) 75, 87, 561, 701, 854
ApeKI GCWGC 5 cut(s) 199, 533, 551, 710, 761
ApoI RAATTY 3 cut(s) 170, 371, 439
AspLEI GCGC 1 cut(s) 892
AspS9I GGNCC 3 cut(s) 76, 502, 702
AsuHPI GGTGA 1 cut(s) 448
AvaII GGWCC 1 cut(s) 502
BanII GRGCYC 1 cut(s) 471
Bbv12I GWGCWC 1 cut(s) 471
BbvCI CCTCAGC 1 cut(s) 765
BbvI GCAGC 5 cut(s) 211, 520, 538, 697, 773
BccI CCATC 4 cut(s) 53, 185, 679, 829
BciT130I CCWGG 1 cut(s) 80
BclI TGATCA 1 cut(s) 621
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 2 cut(s) 12, 194
BfmI CTRYAG 2 cut(s) 162, 723
BfrI CTTAAG 2 cut(s) 65, 473
BisI GCNGC 6 cut(s) 200, 224, 534, 552, 711, 762
BlsI GCNGC 6 cut(s) 201, 225, 535, 553, 712, 763
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 1 cut(s) 80
Bme18I GGWCC 1 cut(s) 502
BmgT120I GGNCC 3 cut(s) 76, 502, 702
BmiI GGNNCC 1 cut(s) 397
BmrFI CCNGG 1 cut(s) 80
BmsI GCATC 2 cut(s) 649, 789
BpmI CTGGAG 1 cut(s) 770
Bpu10I CCTNAGC 1 cut(s) 765
BpuEI CTTGAG 4 cut(s) 287, 422, 542, 698
BsaJI CCNNGG 2 cut(s) 78, 90
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 478
Bse118I RCCGGY 1 cut(s) 536
Bse3DI GCAATG 2 cut(s) 321, 885
BseBI CCWGG 1 cut(s) 80
BseDI CCNNGG 2 cut(s) 78, 90
BseGI GGATG 2 cut(s) 64, 796
BseLI CCNNNNNNNGG 2 cut(s) 85, 478
BseMI GCAATG 2 cut(s) 321, 885
BseMII CTCAG 2 cut(s) 210, 756
BseRI GAGGAG 2 cut(s) 782, 785
BseXI GCAGC 5 cut(s) 211, 520, 538, 697, 773
Bsh1236I CGCG 1 cut(s) 335
BshFI GGCC 5 cut(s) 77, 89, 563, 703, 856
BsiHKAI GWGCWC 1 cut(s) 471
BsiSI CCGG 1 cut(s) 537
BslFI GGGAC 2 cut(s) 131, 642
BslI CCNNNNNNNGG 2 cut(s) 85, 478
BsmFI GGGAC 2 cut(s) 131, 642
BsnI GGCC 5 cut(s) 77, 89, 563, 703, 856
Bsp1286I GDGCHC 1 cut(s) 471
Bsp1407I TGTACA 1 cut(s) 153
Bsp143I GATC 2 cut(s) 298, 621
BspACI CCGC 3 cut(s) 224, 232, 500
BspANI GGCC 5 cut(s) 77, 89, 563, 703, 856
BspCNI CTCAG 2 cut(s) 211, 757
BspFNI CGCG 1 cut(s) 335
BspLI GGNNCC 1 cut(s) 397
BspMAI CTGCAG 1 cut(s) 727
BspQI GCTCTTC 1 cut(s) 459
BspTI CTTAAG 2 cut(s) 65, 473
BsrDI GCAATG 2 cut(s) 321, 885
BsrFI RCCGGY 1 cut(s) 536
BsrGI TGTACA 1 cut(s) 153
BssAI RCCGGY 1 cut(s) 536
BssECI CCNNGG 2 cut(s) 78, 90
BssMI GATC 2 cut(s) 298, 621
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 80
Bst4CI ACNGT 3 cut(s) 395, 653, 783
Bst6I CTCTTC 2 cut(s) 292, 459
BstAFI CTTAAG 2 cut(s) 65, 473
BstAUI TGTACA 1 cut(s) 153
BstC8I GCNNGC 1 cut(s) 538
BstDEI CTNAG 2 cut(s) 219, 765
BstENI CCTNNNNNAGG 2 cut(s) 83, 476
BstF5I GGATG 2 cut(s) 64, 796
BstFNI CGCG 1 cut(s) 335
BstHHI GCGC 1 cut(s) 892
BstKTI GATC 2 cut(s) 301, 624
BstMBI GATC 2 cut(s) 298, 621
BstMWI GCNNNNNNNGC 2 cut(s) 173, 560
BstNI CCWGG 1 cut(s) 80
BstNSI RCATGY 2 cut(s) 314, 577
BstSCI CCNGG 1 cut(s) 78
BstSFI CTRYAG 2 cut(s) 162, 723
BstUI CGCG 1 cut(s) 335
BstV1I GCAGC 5 cut(s) 211, 520, 538, 697, 773
BsuRI GGCC 5 cut(s) 77, 89, 563, 703, 856
BtsCI GGATG 2 cut(s) 64, 796
BtsI GCAGTG 3 cut(s) 633, 720, 732
BtsIMutI CAGTG 3 cut(s) 633, 720, 732
Cac8I GCNNGC 1 cut(s) 538
CaiI CAGNNNCTG 1 cut(s) 584
CfoI GCGC 1 cut(s) 892
Cfr10I RCCGGY 1 cut(s) 536
Cfr13I GGNCC 3 cut(s) 76, 502, 702
CseI GACGC 1 cut(s) 341
Csp6I GTAC 2 cut(s) 9, 154
CspCI CAANNNNNGTGG 2 cut(s) 634, 669
CviAII CATG 5 cut(s) 109, 311, 361, 379, 574
CviQI GTAC 2 cut(s) 9, 154
DdeI CTNAG 2 cut(s) 219, 765
DpnI GATC 2 cut(s) 300, 623
DpnII GATC 2 cut(s) 298, 621
Eam1104I CTCTTC 2 cut(s) 292, 459
EarI CTCTTC 2 cut(s) 292, 459
Ecl136II GAGCTC 1 cut(s) 469
Eco130I CCWWGG 1 cut(s) 90
Eco147I AGGCCT 2 cut(s) 89, 563
Eco24I GRGCYC 1 cut(s) 471
Eco47I GGWCC 1 cut(s) 502
Eco53kI GAGCTC 1 cut(s) 469
Eco57I CTGAAG 1 cut(s) 483
EcoICRI GAGCTC 1 cut(s) 469
EcoNI CCTNNNNNAGG 2 cut(s) 83, 476
EcoO109I RGGNCCY 1 cut(s) 76
EcoRI GAATTC 1 cut(s) 439
EcoRII CCWGG 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 90
EcoT22I ATGCAT 1 cut(s) 804
EcoT38I GRGCYC 1 cut(s) 471
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 5 cut(s) 112, 314, 364, 382, 577
FalI AAGNNNNNCTT 6 cut(s) 114, 146, 456, 488, 656, 688
FaqI GGGAC 2 cut(s) 131, 642
FatI CATG 5 cut(s) 108, 310, 360, 378, 573
FauI CCCGC 1 cut(s) 239
FbaI TGATCA 1 cut(s) 621
Fnu4HI GCNGC 6 cut(s) 200, 224, 534, 552, 711, 762
FokI GGATG 2 cut(s) 71, 803
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 6 cut(s) 200, 224, 534, 552, 711, 762
FspBI CTAG 2 cut(s) 12, 194
GlaI GCGC 1 cut(s) 891
GluI GCNGC 6 cut(s) 200, 224, 534, 552, 711, 762
GsuI CTGGAG 1 cut(s) 770
HaeIII GGCC 5 cut(s) 77, 89, 563, 703, 856
HapII CCGG 1 cut(s) 537
HgaI GACGC 1 cut(s) 341
HhaI GCGC 1 cut(s) 892
Hin1II CATG 5 cut(s) 112, 314, 364, 382, 577
Hin6I GCGC 1 cut(s) 890
HinP1I GCGC 1 cut(s) 890
HinfI GANTC 4 cut(s) 112, 125, 382, 630
HpaII CCGG 1 cut(s) 537
HphI GGTGA 1 cut(s) 448
Hpy166II GTNNAC 2 cut(s) 214, 505
Hpy188I TCNGA 1 cut(s) 220
Hpy188III TCNNGA 3 cut(s) 43, 116, 386
Hpy8I GTNNAC 2 cut(s) 214, 505
HpyAV CCTTC 5 cut(s) 140, 472, 846, 867, 898
HpyCH4III ACNGT 3 cut(s) 395, 653, 783
HpyCH4V TGCA 6 cut(s) 314, 614, 725, 743, 802, 824
HpyF10VI GCNNNNNNNGC 2 cut(s) 173, 560
HpyF3I CTNAG 2 cut(s) 219, 765
Hsp92II CATG 5 cut(s) 112, 314, 364, 382, 577
HspAI GCGC 1 cut(s) 890
KroI GCCGGC 1 cut(s) 536
KroNI GCCGGC 1 cut(s) 538
Ksp22I TGATCA 1 cut(s) 621
Kzo9I GATC 2 cut(s) 298, 621
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 2 cut(s) 474, 598
LpnPI CCDG 8 cut(s) 65, 92, 371, 429, 504, 550, 570, 734
Lsp1109I GCAGC 5 cut(s) 211, 520, 538, 697, 773
LweI GCATC 2 cut(s) 649, 789
MaeI CTAG 2 cut(s) 12, 194
MalI GATC 2 cut(s) 300, 623
MboI GATC 2 cut(s) 298, 621
MboII GAAGA 5 cut(s) 18, 107, 279, 415, 476
MhlI GDGCHC 1 cut(s) 471
MluCI AATT 7 cut(s) 22, 170, 204, 371, 427, 439, 867
MlyI GAGTC 2 cut(s) 119, 624
Mph1103I ATGCAT 1 cut(s) 804
MroNI GCCGGC 1 cut(s) 536
MseI TTAA 4 cut(s) 66, 207, 474, 866
MslI CAYNNNNRTG 3 cut(s) 138, 510, 725
MspA1I CMGCKG 1 cut(s) 764
MspCI CTTAAG 2 cut(s) 65, 473
MspI CCGG 1 cut(s) 537
MspR9I CCNGG 1 cut(s) 80
MvaI CCWGG 1 cut(s) 80
MvnI CGCG 1 cut(s) 335
MwoI GCNNNNNNNGC 2 cut(s) 173, 560
NaeI GCCGGC 1 cut(s) 538
NdeII GATC 2 cut(s) 298, 621
NgoMIV GCCGGC 1 cut(s) 536
NlaIII CATG 5 cut(s) 112, 314, 364, 382, 577
NlaIV GGNNCC 1 cut(s) 397
NsiI ATGCAT 1 cut(s) 804
NspI RCATGY 2 cut(s) 314, 577
OliI CACNNNNGTG 1 cut(s) 725
PceI AGGCCT 2 cut(s) 89, 563
PciI ACATGT 1 cut(s) 573
PciSI GCTCTTC 1 cut(s) 459
PdiI GCCGGC 1 cut(s) 538
PfeI GAWTC 2 cut(s) 112, 382
PkrI GCNGC 6 cut(s) 201, 225, 535, 553, 712, 763
PleI GAGTC 2 cut(s) 119, 624
PpsI GAGTC 2 cut(s) 119, 624
PscI ACATGT 1 cut(s) 573
Psp124BI GAGCTC 1 cut(s) 471
Psp6I CCWGG 1 cut(s) 78
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 1 cut(s) 397
PspPI GGNCC 3 cut(s) 76, 502, 702
PstI CTGCAG 1 cut(s) 727
PstNI CAGNNNCTG 1 cut(s) 584
PvuII CAGCTG 1 cut(s) 764
RsaI GTAC 2 cut(s) 10, 155
RsaNI GTAC 2 cut(s) 9, 154
RseI CAYNNNNRTG 3 cut(s) 138, 510, 725
SacI GAGCTC 1 cut(s) 471
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 4 cut(s) 66, 207, 474, 866
SatI GCNGC 6 cut(s) 200, 224, 534, 552, 711, 762
Sau3AI GATC 2 cut(s) 298, 621
Sau96I GGNCC 3 cut(s) 76, 502, 702
ScaI AGTACT 1 cut(s) 10
SchI GAGTC 2 cut(s) 119, 624
ScrFI CCNGG 1 cut(s) 80
SduI GDGCHC 1 cut(s) 471
SfaNI GCATC 2 cut(s) 649, 789
SfcI CTRYAG 2 cut(s) 162, 723
SinI GGWCC 1 cut(s) 502
SmiMI CAYNNNNRTG 3 cut(s) 138, 510, 725
SmlI CTYRAG 6 cut(s) 65, 302, 401, 473, 557, 677
SmoI CTYRAG 6 cut(s) 65, 302, 401, 473, 557, 677
SpeI ACTAGT 1 cut(s) 11
Sse9I AATT 7 cut(s) 22, 170, 204, 371, 427, 439, 867
SseBI AGGCCT 2 cut(s) 89, 563
SsiI CCGC 3 cut(s) 224, 232, 500
SspMI CTAG 2 cut(s) 12, 194
SstI GAGCTC 1 cut(s) 471
StuI AGGCCT 2 cut(s) 89, 563
StyD4I CCNGG 1 cut(s) 78
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 3 cut(s) 395, 653, 783
TasI AATT 7 cut(s) 22, 170, 204, 371, 427, 439, 867
TatI WGTACW 2 cut(s) 8, 153
TauI GCSGC 1 cut(s) 226
TfiI GAWTC 2 cut(s) 112, 382
Tru1I TTAA 4 cut(s) 66, 207, 474, 866
Tru9I TTAA 4 cut(s) 66, 207, 474, 866
TscAI CASTG 3 cut(s) 640, 727, 732
TseI GCWGC 5 cut(s) 199, 533, 551, 710, 761
TspDTI ATGAA 3 cut(s) 201, 377, 471
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 3 cut(s) 640, 727, 732
Vha464I CTTAAG 2 cut(s) 65, 473
VpaK11BI GGWCC 1 cut(s) 502
XagI CCTNNNNNAGG 2 cut(s) 83, 476
XapI RAATTY 3 cut(s) 170, 371, 439
XceI RCATGY 2 cut(s) 314, 577
XspI CTAG 2 cut(s) 12, 194
ZrmI AGTACT 1 cut(s) 10
Zsp2I ATGCAT 1 cut(s) 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.