RLG00000020513

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001047
Physical Location & Seq
Reverse (-)
65820506 .. 65823214
2709 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020513

Sequence Viewer

Length: 753 bp
ATGGCTATTGCTCGTCTGGTAGCTAATCGTTTCTTTCTTGTTTTTGCTTTGGCTTTCTTGCTCGGAGCGCCGCCTTGGTATGGGCTCCCTGTGATTACTGATTGGGAGGGTAGTGTTCAAAGCTTGGTGGATCTTCAAGTTGTCTTGGGAGCCTCCGATCGGGTTCATACCGATCTAGGATCTGGTTTCTTCAATGCTTGCGAAGGCACCAGAATGGATCTGAAAGATGGTACAGTATCGTCGGTGGTGACCCACGGGGACGATGGCTATGGGGATGGAGGTTTCCAGGGCGACGGTGTTCCTACTTGGGCTCTCTGGGCTGGTGATCGTTCTGGGGATGGGGATGCTCAACGTGGTGGCTATTTTGGCTATGCTGGTGATGGCCTGAAGGAGGTTAGTAGGGGCGGCGGCGACGGAGACATGCAACTTGGGAGCGGCTTTGTTGTGGAGCAACTGGTTGTTAGGGTTTCAGAAAATTTGGGCTATTGGGCTCAACGTTGGGATCTCGACCTTGGTTTTCGAAGACTCCGGTTGAGGCGATTCTCGGTTGAGGGTGGTTTCTTAAACGTGGTTTGGCAGATCGTGTCAAAGTTGCGACGTTGGTGTCGATGCCTACAGATTTCGGGGCAATTGGACTATGCGTTGTCATGGTTGCTGGAGGCAAAGTTCCTTCAGTGGCGTCGAGCTAGGATCGATGAACTCAACTCTGGCTATGTTGACGCGAATGTGTTGCTCTTTGTGGAAGCGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

251

Amino Acids

27.57

Weight (kDa)

4.99

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 603
AccB1I GGYRCC 1 cut(s) 206
AccBSI CCGCTC 1 cut(s) 435
AccII CGCG 1 cut(s) 722
AciI CCGC 5 cut(s) 71, 405, 408, 435, 746
AclI AACGTT 1 cut(s) 496
AclWI GGATC 5 cut(s) 138, 187, 225, 510, 698
AcsI RAATTY 1 cut(s) 475
AcuI CTGAAG 2 cut(s) 407, 656
AcyI GRCGYC 1 cut(s) 679
AfaI GTAC 1 cut(s) 232
AfiI CCNNNNNNNGG 3 cut(s) 80, 159, 391
AgsI TTSAA 3 cut(s) 119, 137, 193
AjnI CCWGG 1 cut(s) 285
AloI GAACNNNNNNTCC 2 cut(s) 650, 682
AluBI AGCT 3 cut(s) 23, 123, 686
AluI AGCT 3 cut(s) 23, 123, 686
Alw26I GTCTC 1 cut(s) 411
AlwI GGATC 5 cut(s) 138, 187, 225, 510, 698
AoxI GGCC 1 cut(s) 382
ApoI RAATTY 1 cut(s) 475
AspLEI GCGC 1 cut(s) 70
AsuHPI GGTGA 3 cut(s) 259, 335, 389
AsuII TTCGAA 1 cut(s) 520
BanI GGYRCC 1 cut(s) 206
BanII GRGCYC 3 cut(s) 87, 313, 493
BbsI GAAGAC 1 cut(s) 529
BccI CCATC 5 cut(s) 221, 257, 269, 332, 374
BcgI CGANNNNNNTGC 2 cut(s) 712, 746
BciT130I CCWGG 1 cut(s) 287
BcoDI GTCTC 1 cut(s) 411
BfaI CTAG 2 cut(s) 176, 687
BfmI CTRYAG 1 cut(s) 614
BfoI RGCGCY 1 cut(s) 71
BisI GCNGC 5 cut(s) 71, 406, 409, 436, 747
BlsI GCNGC 5 cut(s) 72, 407, 410, 437, 748
Bme1390I CCNGG 1 cut(s) 287
BmiI GGNNCC 3 cut(s) 86, 151, 208
BmrFI CCNGG 1 cut(s) 287
BmsI GCATC 2 cut(s) 334, 599
BpiI GAAGAC 1 cut(s) 529
BpmI CTGGAG 1 cut(s) 677
Bpu14I TTCGAA 1 cut(s) 520
Bsa29I ATCGAT 1 cut(s) 693
BsaHI GRCGYC 1 cut(s) 679
BsaJI CCNNGG 4 cut(s) 74, 253, 286, 511
BsaWI WCCGGW 1 cut(s) 528
BsaXI ACNNNNNCTCC 2 cut(s) 650, 680
Bsc4I CCNNNNNNNGG 3 cut(s) 80, 159, 391
Bse1I ACTGG 1 cut(s) 459
BseBI CCWGG 1 cut(s) 287
BseCI ATCGAT 1 cut(s) 693
BseDI CCNNGG 4 cut(s) 74, 253, 286, 511
BseGI GGATG 3 cut(s) 280, 343, 349
BseLI CCNNNNNNNGG 3 cut(s) 80, 159, 391
BseNI ACTGG 1 cut(s) 459
Bsh1236I CGCG 1 cut(s) 722
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 1 cut(s) 384
BshNI GGYRCC 1 cut(s) 206
BshVI ATCGAT 1 cut(s) 693
BsiEI CGRYCG 1 cut(s) 160
BsiSI CCGG 1 cut(s) 529
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 3 cut(s) 80, 159, 391
BsmAI GTCTC 1 cut(s) 411
BsmFI GGGAC 1 cut(s) 272
BsnI GGCC 1 cut(s) 384
Bsp119I TTCGAA 1 cut(s) 520
Bsp1286I GDGCHC 3 cut(s) 87, 313, 493
Bsp143I GATC 9 cut(s) 130, 157, 172, 179, 217, 325, 502, 579, 690
BspACI CCGC 5 cut(s) 71, 405, 408, 435, 746
BspANI GGCC 1 cut(s) 384
BspDI ATCGAT 1 cut(s) 693
BspFNI CGCG 1 cut(s) 722
BspLI GGNNCC 3 cut(s) 86, 151, 208
BspPI GGATC 5 cut(s) 138, 187, 225, 510, 698
BspT104I TTCGAA 1 cut(s) 520
BspT107I GGYRCC 1 cut(s) 206
BsrBI CCGCTC 1 cut(s) 435
BsrI ACTGG 1 cut(s) 459
BssECI CCNNGG 4 cut(s) 74, 253, 286, 511
BssMI GATC 9 cut(s) 130, 157, 172, 179, 217, 325, 502, 579, 690
BssNI GRCGYC 1 cut(s) 679
BssT1I CCWWGG 2 cut(s) 74, 511
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 2 cut(s) 235, 296
BstACI GRCGYC 1 cut(s) 679
BstBI TTCGAA 1 cut(s) 520
BstC8I GCNNGC 1 cut(s) 199
BstDSI CCRYGG 1 cut(s) 253
BstEII GGTNACC 1 cut(s) 247
BstENI CCTNNNNNAGG 1 cut(s) 389
BstF5I GGATG 3 cut(s) 280, 343, 349
BstFNI CGCG 1 cut(s) 722
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 9 cut(s) 133, 160, 175, 182, 220, 328, 505, 582, 693
BstMAI GTCTC 1 cut(s) 411
BstMBI GATC 9 cut(s) 130, 157, 172, 179, 217, 325, 502, 579, 690
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 3 cut(s) 67, 317, 366
BstNI CCWGG 1 cut(s) 287
BstNSI RCATGY 1 cut(s) 424
BstPI GGTNACC 1 cut(s) 247
BstSCI CCNGG 1 cut(s) 285
BstSFI CTRYAG 1 cut(s) 614
BstUI CGCG 1 cut(s) 722
BstV2I GAAGAC 1 cut(s) 529
BstX2I RGATCY 4 cut(s) 130, 179, 217, 502
BstYI RGATCY 4 cut(s) 130, 179, 217, 502
Bsu15I ATCGAT 1 cut(s) 693
BsuRI GGCC 1 cut(s) 384
BsuTUI ATCGAT 1 cut(s) 693
BtgI CCRYGG 1 cut(s) 253
BtsCI GGATG 3 cut(s) 280, 343, 349
BtsIMutI CAGTG 1 cut(s) 680
Cac8I GCNNGC 1 cut(s) 199
CfoI GCGC 1 cut(s) 70
ClaI ATCGAT 1 cut(s) 693
CseI GACGC 2 cut(s) 668, 728
Csp6I GTAC 1 cut(s) 231
CviAII CATG 3 cut(s) 421, 648, 750
CviQI GTAC 1 cut(s) 231
DpnI GATC 9 cut(s) 132, 159, 174, 181, 219, 327, 504, 581, 692
DpnII GATC 9 cut(s) 130, 157, 172, 179, 217, 325, 502, 579, 690
DrdI GACNNNNNNGTC 1 cut(s) 603
DseDI GACNNNNNNGTC 1 cut(s) 603
Eco130I CCWWGG 2 cut(s) 74, 511
Eco24I GRGCYC 3 cut(s) 87, 313, 493
Eco57I CTGAAG 2 cut(s) 407, 656
Eco91I GGTNACC 1 cut(s) 247
EcoNI CCTNNNNNAGG 1 cut(s) 389
EcoO65I GGTNACC 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 285
EcoT14I CCWWGG 2 cut(s) 74, 511
EcoT38I GRGCYC 3 cut(s) 87, 313, 493
ErhI CCWWGG 2 cut(s) 74, 511
FaeI CATG 3 cut(s) 424, 651, 753
FaiI YATR 9 cut(s) 81, 168, 270, 372, 422, 639, 649, 714, 751
FaqI GGGAC 1 cut(s) 272
FatI CATG 3 cut(s) 420, 647, 749
Fnu4HI GCNGC 5 cut(s) 71, 406, 409, 436, 747
FokI GGATG 3 cut(s) 287, 350, 356
FriOI GRGCYC 3 cut(s) 87, 313, 493
Fsp4HI GCNGC 5 cut(s) 71, 406, 409, 436, 747
FspBI CTAG 2 cut(s) 176, 687
GlaI GCGC 1 cut(s) 69
GluI GCNGC 5 cut(s) 71, 406, 409, 436, 747
GsuI CTGGAG 1 cut(s) 677
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 1 cut(s) 384
HapII CCGG 1 cut(s) 529
HgaI GACGC 2 cut(s) 668, 728
HhaI GCGC 1 cut(s) 70
Hin1I GRCGYC 1 cut(s) 679
Hin1II CATG 3 cut(s) 424, 651, 753
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HincII GTYRAC 1 cut(s) 718
HindII GTYRAC 1 cut(s) 718
HindIII AAGCTT 1 cut(s) 121
HinfI GANTC 2 cut(s) 525, 540
HpaII CCGG 1 cut(s) 529
HphI GGTGA 3 cut(s) 259, 335, 389
Hpy166II GTNNAC 1 cut(s) 718
Hpy188I TCNGA 4 cut(s) 65, 157, 222, 472
Hpy188III TCNNGA 1 cut(s) 506
Hpy8I GTNNAC 1 cut(s) 718
Hpy99I CGWCG 5 cut(s) 244, 296, 416, 600, 684
HpyAV CCTTC 3 cut(s) 197, 382, 680
HpyCH4III ACNGT 2 cut(s) 235, 296
HpyCH4IV ACGT 4 cut(s) 352, 496, 567, 598
HpyCH4V TGCA 1 cut(s) 424
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 317, 366
HpySE526I ACGT 4 cut(s) 352, 496, 567, 598
Hsp92I GRCGYC 1 cut(s) 679
Hsp92II CATG 3 cut(s) 424, 651, 753
HspAI GCGC 1 cut(s) 68
Kzo9I GATC 9 cut(s) 130, 157, 172, 179, 217, 325, 502, 579, 690
LmnI GCTCC 5 cut(s) 65, 90, 149, 432, 448
LweI GCATC 2 cut(s) 334, 599
MaeI CTAG 2 cut(s) 176, 687
MaeII ACGT 4 cut(s) 352, 496, 567, 598
MaeIII GTNAC 1 cut(s) 247
MalI GATC 9 cut(s) 132, 159, 174, 181, 219, 327, 504, 581, 692
MbiI CCGCTC 1 cut(s) 435
MboI GATC 9 cut(s) 130, 157, 172, 179, 217, 325, 502, 579, 690
MboII GAAGA 3 cut(s) 125, 181, 534
MfeI CAATTG 1 cut(s) 629
MflI RGATCY 4 cut(s) 130, 179, 217, 502
MhlI GDGCHC 3 cut(s) 87, 313, 493
MluCI AATT 2 cut(s) 475, 629
MlyI GAGTC 1 cut(s) 519
MnlI CCTC 7 cut(s) 100, 163, 272, 385, 528, 544, 652
MseI TTAA 1 cut(s) 563
MslI CAYNNNNRTG 1 cut(s) 212
MspI CCGG 1 cut(s) 529
MspR9I CCNGG 1 cut(s) 287
MunI CAATTG 1 cut(s) 629
MvaI CCWGG 1 cut(s) 287
MvnI CGCG 1 cut(s) 722
MwoI GCNNNNNNNGC 3 cut(s) 67, 317, 366
NdeII GATC 9 cut(s) 130, 157, 172, 179, 217, 325, 502, 579, 690
NlaIII CATG 3 cut(s) 424, 651, 753
NlaIV GGNNCC 3 cut(s) 86, 151, 208
NmuCI GTSAC 1 cut(s) 247
NspI RCATGY 1 cut(s) 424
NspV TTCGAA 1 cut(s) 520
PcsI WCGNNNNNNNCGW 1 cut(s) 604
PfeI GAWTC 1 cut(s) 540
PkrI GCNGC 5 cut(s) 72, 407, 410, 437, 748
Ple19I CGATCG 1 cut(s) 160
PleI GAGTC 1 cut(s) 519
PpsI GAGTC 1 cut(s) 519
Psp1406I AACGTT 1 cut(s) 496
Psp6I CCWGG 1 cut(s) 285
PspEI GGTNACC 1 cut(s) 247
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 3 cut(s) 86, 151, 208
PsuI RGATCY 4 cut(s) 130, 179, 217, 502
PvuI CGATCG 1 cut(s) 160
RsaI GTAC 1 cut(s) 232
RsaNI GTAC 1 cut(s) 231
RseI CAYNNNNRTG 1 cut(s) 212
SaqAI TTAA 1 cut(s) 563
SatI GCNGC 5 cut(s) 71, 406, 409, 436, 747
Sau3AI GATC 9 cut(s) 130, 157, 172, 179, 217, 325, 502, 579, 690
SchI GAGTC 1 cut(s) 519
ScrFI CCNGG 1 cut(s) 287
SduI GDGCHC 3 cut(s) 87, 313, 493
SfaNI GCATC 2 cut(s) 334, 599
SfcI CTRYAG 1 cut(s) 614
SfuI TTCGAA 1 cut(s) 520
SmiMI CAYNNNNRTG 1 cut(s) 212
Sse9I AATT 2 cut(s) 475, 629
SsiI CCGC 5 cut(s) 71, 405, 408, 435, 746
SspMI CTAG 2 cut(s) 176, 687
StyD4I CCNGG 1 cut(s) 285
StyI CCWWGG 2 cut(s) 74, 511
TaaI ACNGT 2 cut(s) 235, 296
TaiI ACGT 4 cut(s) 355, 499, 570, 601
TaqI TCGA 5 cut(s) 507, 520, 607, 682, 693
TasI AATT 2 cut(s) 475, 629
TauI GCSGC 5 cut(s) 73, 408, 411, 438, 749
TfiI GAWTC 1 cut(s) 540
Tru1I TTAA 1 cut(s) 563
Tru9I TTAA 1 cut(s) 563
TscAI CASTG 1 cut(s) 680
TseFI GTSAC 1 cut(s) 247
Tsp45I GTSAC 1 cut(s) 247
TspDTI ATGAA 2 cut(s) 155, 711
TspGWI ACGGA 1 cut(s) 429
TspRI CASTG 1 cut(s) 680
XagI CCTNNNNNAGG 1 cut(s) 389
XapI RAATTY 1 cut(s) 475
XceI RCATGY 1 cut(s) 424
XcmI CCANNNNNNNNNTGG 1 cut(s) 260
XspI CTAG 2 cut(s) 176, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.