component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 117 |
| AcsI | RAATTY | 3 cut(s) | 43, 126, 282 |
| AcyI | GRCGYC | 1 cut(s) | 120 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 72 |
| AgsI | TTSAA | 1 cut(s) | 113 |
| AluBI | AGCT | 1 cut(s) | 179 |
| AluI | AGCT | 1 cut(s) | 179 |
| ApoI | RAATTY | 3 cut(s) | 43, 126, 282 |
| BfaI | CTAG | 1 cut(s) | 104 |
| BfmI | CTRYAG | 1 cut(s) | 162 |
| BglII | AGATCT | 2 cut(s) | 158, 233 |
| BisI | GCNGC | 1 cut(s) | 118 |
| BlsI | GCNGC | 1 cut(s) | 119 |
| BmiI | GGNNCC | 1 cut(s) | 228 |
| BsaHI | GRCGYC | 1 cut(s) | 120 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 72 |
| Bse3DI | GCAATG | 1 cut(s) | 23 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 72 |
| BseMI | GCAATG | 1 cut(s) | 23 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 72 |
| BsmI | GAATGC | 1 cut(s) | 119 |
| Bsp143I | GATC | 2 cut(s) | 158, 233 |
| BspACI | CCGC | 1 cut(s) | 117 |
| BspLI | GGNNCC | 1 cut(s) | 228 |
| BsrDI | GCAATG | 1 cut(s) | 23 |
| BssMI | GATC | 2 cut(s) | 158, 233 |
| BssNI | GRCGYC | 1 cut(s) | 120 |
| Bst4CI | ACNGT | 1 cut(s) | 260 |
| BstACI | GRCGYC | 1 cut(s) | 120 |
| BstC8I | GCNNGC | 2 cut(s) | 100, 177 |
| BstKTI | GATC | 2 cut(s) | 161, 236 |
| BstMBI | GATC | 2 cut(s) | 158, 233 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 185 |
| BstSFI | CTRYAG | 1 cut(s) | 162 |
| BstX2I | RGATCY | 2 cut(s) | 158, 233 |
| BstYI | RGATCY | 2 cut(s) | 158, 233 |
| Cac8I | GCNNGC | 2 cut(s) | 100, 177 |
| CseI | GACGC | 1 cut(s) | 109 |
| CviAII | CATG | 2 cut(s) | 200, 211 |
| CviJI | RGCY | 3 cut(s) | 102, 179, 188 |
| CviKI_1 | RGCY | 3 cut(s) | 102, 179, 188 |
| DpnI | GATC | 2 cut(s) | 160, 235 |
| DpnII | GATC | 2 cut(s) | 158, 233 |
| DraI | TTTAAA | 1 cut(s) | 40 |
| FaeI | CATG | 2 cut(s) | 203, 214 |
| FaiI | YATR | 4 cut(s) | 72, 192, 201, 212 |
| FatI | CATG | 2 cut(s) | 199, 210 |
| Fnu4HI | GCNGC | 1 cut(s) | 118 |
| Fsp4HI | GCNGC | 1 cut(s) | 118 |
| FspBI | CTAG | 1 cut(s) | 104 |
| GluI | GCNGC | 1 cut(s) | 118 |
| HgaI | GACGC | 1 cut(s) | 109 |
| Hin1I | GRCGYC | 1 cut(s) | 120 |
| Hin1II | CATG | 2 cut(s) | 203, 214 |
| HinfI | GANTC | 2 cut(s) | 51, 91 |
| Hpy188III | TCNNGA | 1 cut(s) | 48 |
| HpyAV | CCTTC | 2 cut(s) | 16, 257 |
| HpyCH4III | ACNGT | 1 cut(s) | 260 |
| HpyCH4V | TGCA | 1 cut(s) | 194 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 185 |
| Hsp92I | GRCGYC | 1 cut(s) | 120 |
| Hsp92II | CATG | 2 cut(s) | 203, 214 |
| Kzo9I | GATC | 2 cut(s) | 158, 233 |
| LpnPI | CCDG | 4 cut(s) | 33, 80, 150, 244 |
| MaeI | CTAG | 1 cut(s) | 104 |
| MaeIII | GTNAC | 1 cut(s) | 152 |
| MalI | GATC | 2 cut(s) | 160, 235 |
| MboI | GATC | 2 cut(s) | 158, 233 |
| MflI | RGATCY | 2 cut(s) | 158, 233 |
| MluCI | AATT | 3 cut(s) | 43, 126, 282 |
| MnlI | CCTC | 1 cut(s) | 241 |
| MseI | TTAA | 2 cut(s) | 39, 289 |
| Mva1269I | GAATGC | 1 cut(s) | 119 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 185 |
| NdeII | GATC | 2 cut(s) | 158, 233 |
| NlaIII | CATG | 2 cut(s) | 203, 214 |
| NlaIV | GGNNCC | 1 cut(s) | 228 |
| NmuCI | GTSAC | 1 cut(s) | 152 |
| PctI | GAATGC | 1 cut(s) | 119 |
| PfeI | GAWTC | 2 cut(s) | 51, 91 |
| PkrI | GCNGC | 1 cut(s) | 119 |
| PspN4I | GGNNCC | 1 cut(s) | 228 |
| PsuI | RGATCY | 2 cut(s) | 158, 233 |
| SaqAI | TTAA | 2 cut(s) | 39, 289 |
| SatI | GCNGC | 1 cut(s) | 118 |
| Sau3AI | GATC | 2 cut(s) | 158, 233 |
| SetI | ASST | 5 cut(s) | 8, 169, 181, 228, 268 |
| SfcI | CTRYAG | 1 cut(s) | 162 |
| Sse9I | AATT | 3 cut(s) | 43, 126, 282 |
| SsiI | CCGC | 1 cut(s) | 117 |
| SspMI | CTAG | 1 cut(s) | 104 |
| TaaI | ACNGT | 1 cut(s) | 260 |
| TasI | AATT | 3 cut(s) | 43, 126, 282 |
| TauI | GCSGC | 1 cut(s) | 120 |
| TfiI | GAWTC | 2 cut(s) | 51, 91 |
| Tru1I | TTAA | 2 cut(s) | 39, 289 |
| Tru9I | TTAA | 2 cut(s) | 39, 289 |
| TseFI | GTSAC | 1 cut(s) | 152 |
| Tsp45I | GTSAC | 1 cut(s) | 152 |
| TspDTI | ATGAA | 1 cut(s) | 188 |
| XapI | RAATTY | 3 cut(s) | 43, 126, 282 |
| XspI | CTAG | 1 cut(s) | 104 |