Rh4DG240200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001047
Physical Location & Seq
Forward (+)
44878066 .. 44878398
333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG240200.1

Sequence Viewer

Length: 333 bp
ATGACTGATCGGAGGCCGAGCTCTAACAGCGTGGATCTGGTTTGGACTGAGTTGAATGGGCGAGTCTGGGTTTTGGATCGGGGTCGTCAGATCTCCGACGGTGATAGGGATTGTACAATCGCTTTGGTTGGCCCTGGTGGTTCGCTGCAGCGTAGTGGTGTTGCCGGAGTGGGGTTGGCCAGACCAGTTCAGGGGATAGATCAAAGCTTGGAGGATCTTCAGGTTGTCCTGGGCGCCTCCGATCAGATTCATCCCGATCTAGGATCTGGTTTCTTCAATGATTGCGAAGGCACTGGAATGGATCTGAAGTTGGTTCGAGGTGATCCGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

110

Amino Acids

11.72

Weight (kDa)

4.62

Isoelectric Point (pI)

31.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 233
AclWI GGATC 6 cut(s) 42, 84, 222, 271, 309, 317
AcoI YGGCCR 1 cut(s) 177
AcuI CTGAAG 2 cut(s) 203, 326
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 1 cut(s) 115
AfiI CCNNNNNNNGG 3 cut(s) 171, 191, 260
AgsI TTSAA 2 cut(s) 55, 277
AjnI CCWGG 2 cut(s) 133, 228
AluBI AGCT 2 cut(s) 21, 207
AluI AGCT 2 cut(s) 21, 207
Alw21I GWGCWC 1 cut(s) 23
AlwI GGATC 6 cut(s) 42, 84, 222, 271, 309, 317
AoxI GGCC 3 cut(s) 14, 130, 177
ApeKI GCWGC 2 cut(s) 145, 148
AspLEI GCGC 1 cut(s) 236
AspS9I GGNCC 1 cut(s) 131
AsuHPI GGTGA 2 cut(s) 113, 332
BalI TGGCCA 1 cut(s) 179
BanI GGYRCC 1 cut(s) 233
BanII GRGCYC 1 cut(s) 23
Bbv12I GWGCWC 1 cut(s) 23
BbvI GCAGC 2 cut(s) 132, 160
BciT130I CCWGG 2 cut(s) 135, 230
BfaI CTAG 1 cut(s) 260
BfmI CTRYAG 1 cut(s) 146
BfoI RGCGCY 1 cut(s) 237
BglII AGATCT 1 cut(s) 90
BisI GCNGC 2 cut(s) 146, 149
BlsI GCNGC 2 cut(s) 147, 150
Bme1390I CCNGG 2 cut(s) 135, 230
BmgT120I GGNCC 1 cut(s) 131
BmiI GGNNCC 1 cut(s) 235
BmrFI CCNGG 2 cut(s) 135, 230
BsaHI GRCGYC 1 cut(s) 234
BsaJI CCNNGG 2 cut(s) 133, 229
Bsc4I CCNNNNNNNGG 3 cut(s) 171, 191, 260
Bse1I ACTGG 2 cut(s) 185, 298
BseBI CCWGG 2 cut(s) 135, 230
BseDI CCNNGG 2 cut(s) 133, 229
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 3 cut(s) 171, 191, 260
BseMII CTCAG 1 cut(s) 39
BseNI ACTGG 2 cut(s) 185, 298
BseXI GCAGC 2 cut(s) 132, 160
BshFI GGCC 3 cut(s) 16, 132, 179
BshNI GGYRCC 1 cut(s) 233
BsiHKAI GWGCWC 1 cut(s) 23
BsiSI CCGG 1 cut(s) 165
BslI CCNNNNNNNGG 3 cut(s) 171, 191, 260
BsnI GGCC 3 cut(s) 16, 132, 179
Bsp1286I GDGCHC 1 cut(s) 23
Bsp1407I TGTACA 1 cut(s) 113
BspANI GGCC 3 cut(s) 16, 132, 179
BspCNI CTCAG 1 cut(s) 40
BspLI GGNNCC 1 cut(s) 235
BspMAI CTGCAG 1 cut(s) 150
BspPI GGATC 6 cut(s) 42, 84, 222, 271, 309, 317
BspT107I GGYRCC 1 cut(s) 233
BsrGI TGTACA 1 cut(s) 113
BsrI ACTGG 2 cut(s) 185, 298
BssECI CCNNGG 2 cut(s) 133, 229
BssNI GRCGYC 1 cut(s) 234
Bst2UI CCWGG 2 cut(s) 135, 230
Bst4CI ACNGT 1 cut(s) 101
BstACI GRCGYC 1 cut(s) 234
BstAUI TGTACA 1 cut(s) 113
BstDEI CTNAG 1 cut(s) 48
BstF5I GGATG 1 cut(s) 250
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 1 cut(s) 236
BstMWI GCNNNNNNNGC 1 cut(s) 27
BstNI CCWGG 2 cut(s) 135, 230
BstSCI CCNGG 2 cut(s) 133, 228
BstSFI CTRYAG 1 cut(s) 146
BstV1I GCAGC 2 cut(s) 132, 160
BstX2I RGATCY 5 cut(s) 34, 90, 214, 263, 301
BstYI RGATCY 5 cut(s) 34, 90, 214, 263, 301
BsuRI GGCC 3 cut(s) 16, 132, 179
BtsCI GGATG 1 cut(s) 250
BtsIMutI CAGTG 1 cut(s) 291
CfoI GCGC 1 cut(s) 236
Cfr13I GGNCC 1 cut(s) 131
Csp6I GTAC 1 cut(s) 114
CviJI RGCY 5 cut(s) 16, 21, 132, 179, 207
CviKI_1 RGCY 5 cut(s) 16, 21, 132, 179, 207
CviQI GTAC 1 cut(s) 114
DdeI CTNAG 1 cut(s) 48
DinI GGCGCC 1 cut(s) 235
EaeI YGGCCR 1 cut(s) 177
Ecl136II GAGCTC 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 23
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 2 cut(s) 203, 326
EcoICRI GAGCTC 1 cut(s) 21
EcoRII CCWGG 2 cut(s) 133, 228
EcoT38I GRGCYC 1 cut(s) 23
EgeI GGCGCC 1 cut(s) 235
EheI GGCGCC 1 cut(s) 235
Fnu4HI GCNGC 2 cut(s) 146, 149
FokI GGATG 1 cut(s) 237
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 2 cut(s) 146, 149
FspBI CTAG 1 cut(s) 260
GlaI GCGC 1 cut(s) 235
GluI GCNGC 2 cut(s) 146, 149
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 3 cut(s) 16, 132, 179
HapII CCGG 1 cut(s) 165
HhaI GCGC 1 cut(s) 236
Hin1I GRCGYC 1 cut(s) 234
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HindIII AAGCTT 1 cut(s) 205
HinfI GANTC 2 cut(s) 63, 247
HpaII CCGG 1 cut(s) 165
HphI GGTGA 2 cut(s) 113, 332
Hpy188I TCNGA 7 cut(s) 12, 90, 97, 241, 246, 306, 327
Hpy188III TCNNGA 1 cut(s) 254
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 1 cut(s) 281
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4V TGCA 1 cut(s) 148
HpyF10VI GCNNNNNNNGC 1 cut(s) 27
HpyF3I CTNAG 1 cut(s) 48
Hsp92I GRCGYC 1 cut(s) 234
HspAI GCGC 1 cut(s) 234
KasI GGCGCC 1 cut(s) 233
Lsp1109I GCAGC 2 cut(s) 132, 160
MaeI CTAG 1 cut(s) 260
MboII GAAGA 2 cut(s) 209, 265
MflI RGATCY 5 cut(s) 34, 90, 214, 263, 301
MhlI GDGCHC 1 cut(s) 23
MlsI TGGCCA 1 cut(s) 179
MluNI TGGCCA 1 cut(s) 179
Mly113I GGCGCC 1 cut(s) 234
MlyI GAGTC 1 cut(s) 72
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 4 cut(s) 6, 205, 247, 311
Mox20I TGGCCA 1 cut(s) 179
MscI TGGCCA 1 cut(s) 179
MslI CAYNNNNRTG 1 cut(s) 296
Msp20I TGGCCA 1 cut(s) 179
MspI CCGG 1 cut(s) 165
MspR9I CCNGG 2 cut(s) 135, 230
MvaI CCWGG 2 cut(s) 135, 230
MwoI GCNNNNNNNGC 1 cut(s) 27
NarI GGCGCC 1 cut(s) 234
NlaIV GGNNCC 1 cut(s) 235
NmeAIII GCCGAG 1 cut(s) 42
PfeI GAWTC 1 cut(s) 247
PkrI GCNGC 2 cut(s) 147, 150
PleI GAGTC 1 cut(s) 71
PluTI GGCGCC 1 cut(s) 237
PpsI GAGTC 1 cut(s) 71
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 2 cut(s) 133, 228
PspGI CCWGG 2 cut(s) 133, 228
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 1 cut(s) 131
PstI CTGCAG 1 cut(s) 150
PsuI RGATCY 5 cut(s) 34, 90, 214, 263, 301
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
RseI CAYNNNNRTG 1 cut(s) 296
SacI GAGCTC 1 cut(s) 23
SatI GCNGC 2 cut(s) 146, 149
Sau96I GGNCC 1 cut(s) 131
SchI GAGTC 1 cut(s) 72
ScrFI CCNGG 2 cut(s) 135, 230
SduI GDGCHC 1 cut(s) 23
SetI ASST 4 cut(s) 23, 209, 225, 322
SfcI CTRYAG 1 cut(s) 146
SfoI GGCGCC 1 cut(s) 235
SmiMI CAYNNNNRTG 1 cut(s) 296
SspDI GGCGCC 1 cut(s) 233
SspMI CTAG 1 cut(s) 260
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 2 cut(s) 133, 228
TaaI ACNGT 1 cut(s) 101
TaqI TCGA 1 cut(s) 316
TatI WGTACW 1 cut(s) 113
TfiI GAWTC 1 cut(s) 247
TscAI CASTG 1 cut(s) 298
TseI GCWGC 2 cut(s) 145, 148
TspDTI ATGAA 1 cut(s) 239
TspRI CASTG 1 cut(s) 298
XspI CTAG 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.