Rw2G020930

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001047
Physical Location & Seq
Reverse (-)
28727194 .. 28729268
2075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G020930.1

Sequence Viewer

Length: 780 bp
ATGATTGATCGGAGGCCGAGCTCTAACGGCGTGGATCTGGTTTGGACTGAGTTGGATGGGCGAGTCTGGGTTTTGGATCGGGGTCGTCAGATTTCCGACGGTGATAGGGCTTGTACAATCGCTTTGGTTGGCCCTGGTGGTTCGCTGCAGCGTCATGGTGTTGCCAGAGTGGGGTTGGCCCGACCAGTTCAGGGGATCGATCAAAGCTTGGAGGATCTTCAGGTTGTCCTGGGCGCCTCCGATCGGGTTTATCCCGATCTAGGATCTGGTTTCTTCAATGATTGCGAAGGCACTGGAATGGATCTGAAGTCGGTTCGAGGTGATCAGGAATGGGCGCGAACTTGGGTGTCCTTGGATCATGGAGATGGTACAGTATCGTCGGCGGTGAACCACGGTGACGGCGGCTATGGGGATGGCGGTTTTCAGGGCGATGGAGCTCCTCCTTGGGCTCTATGGGCTGGTGATCGTTCTGGGGATGGGGTTGCTCAATGTGGTGGCTTTTTTGGCTATGCTGGTGATGGCCTGAAGGTGGTCAGTAGGGGAGGCGGCGACGGCGACGGCGACATGAAACTTGGGAGCGGCCATGTTGTGGAGCAGTTGGTTGTTAGGGTTTCAGAAAATTTGGGCTGTTGGGCTCAAGTATTGGGCTTAGTCTGGATGGGGATGCCTTGGGGCCATATGGACTCTGAGGTGGTTATGAAGGATGGCGGTGGTGGAGGTTTGTTGGTCGGCTGGGATAGTAGTGAAGTTGACGAGTTTGATCATAAGGGAGTAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

259

Amino Acids

27.14

Weight (kDa)

4.49

Isoelectric Point (pI)

31.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 233
AccB7I CCANNNNNTGG 1 cut(s) 589
AccBSI CCGCTC 1 cut(s) 579
AccII CGCG 1 cut(s) 337
AciI CCGC 6 cut(s) 383, 402, 417, 546, 579, 708
AclWI GGATC 7 cut(s) 42, 84, 203, 222, 271, 309, 363
AcoI YGGCCR 1 cut(s) 580
AcsI RAATTY 1 cut(s) 619
AcuI CTGAAG 3 cut(s) 203, 326, 545
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 2 cut(s) 115, 370
AfiI CCNNNNNNNGG 6 cut(s) 171, 191, 243, 260, 529, 589
AgsI TTSAA 1 cut(s) 277
AjnI CCWGG 2 cut(s) 133, 228
AluBI AGCT 3 cut(s) 21, 207, 437
AluI AGCT 3 cut(s) 21, 207, 437
Alw21I GWGCWC 2 cut(s) 23, 439
AlwI GGATC 7 cut(s) 42, 84, 203, 222, 271, 309, 363
AoxI GGCC 6 cut(s) 14, 130, 177, 520, 580, 673
ApeKI GCWGC 2 cut(s) 145, 148
ApoI RAATTY 1 cut(s) 619
AspLEI GCGC 2 cut(s) 236, 337
AspS9I GGNCC 3 cut(s) 131, 178, 673
AsuHPI GGTGA 6 cut(s) 113, 332, 397, 407, 473, 527
BanI GGYRCC 1 cut(s) 233
BanII GRGCYC 4 cut(s) 23, 439, 451, 637
Bbv12I GWGCWC 2 cut(s) 23, 439
BbvI GCAGC 2 cut(s) 132, 160
BccI CCATC 8 cut(s) 50, 359, 407, 425, 470, 512, 652, 698
BceAI ACGGC 4 cut(s) 43, 415, 568, 574
BciT130I CCWGG 2 cut(s) 135, 230
BclI TGATCA 2 cut(s) 322, 760
BfaI CTAG 1 cut(s) 260
BfmI CTRYAG 1 cut(s) 146
BfoI RGCGCY 1 cut(s) 237
BisI GCNGC 5 cut(s) 146, 149, 403, 547, 580
BlsI GCNGC 5 cut(s) 147, 150, 404, 548, 581
Bme1390I CCNGG 2 cut(s) 135, 230
BmgT120I GGNCC 3 cut(s) 131, 178, 673
BmiI GGNNCC 2 cut(s) 235, 674
BmrFI CCNGG 2 cut(s) 135, 230
BmsI GCATC 1 cut(s) 654
BpuEI CTTGAG 1 cut(s) 621
Bsa29I ATCGAT 1 cut(s) 198
BsaHI GRCGYC 1 cut(s) 234
BsaJI CCNNGG 6 cut(s) 133, 229, 351, 391, 443, 668
Bsc4I CCNNNNNNNGG 6 cut(s) 171, 191, 243, 260, 529, 589
Bse1I ACTGG 2 cut(s) 185, 298
BseBI CCWGG 2 cut(s) 135, 230
BseCI ATCGAT 1 cut(s) 198
BseDI CCNNGG 6 cut(s) 133, 229, 351, 391, 443, 668
BseGI GGATG 6 cut(s) 61, 418, 481, 663, 669, 709
BseLI CCNNNNNNNGG 6 cut(s) 171, 191, 243, 260, 529, 589
BseMII CTCAG 2 cut(s) 39, 678
BseNI ACTGG 2 cut(s) 185, 298
BseRI GAGGAG 1 cut(s) 429
BseXI GCAGC 2 cut(s) 132, 160
BseYI CCCAGC 1 cut(s) 732
Bsh1236I CGCG 1 cut(s) 337
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 6 cut(s) 16, 132, 179, 522, 582, 675
BshNI GGYRCC 1 cut(s) 233
BshVI ATCGAT 1 cut(s) 198
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 2 cut(s) 23, 439
BslI CCNNNNNNNGG 6 cut(s) 171, 191, 243, 260, 529, 589
BsnI GGCC 6 cut(s) 16, 132, 179, 522, 582, 675
Bsp1286I GDGCHC 4 cut(s) 23, 439, 451, 637
Bsp1407I TGTACA 1 cut(s) 113
BspACI CCGC 6 cut(s) 383, 402, 417, 546, 579, 708
BspANI GGCC 6 cut(s) 16, 132, 179, 522, 582, 675
BspCNI CTCAG 2 cut(s) 40, 679
BspDI ATCGAT 1 cut(s) 198
BspFNI CGCG 1 cut(s) 337
BspLI GGNNCC 2 cut(s) 235, 674
BspMAI CTGCAG 1 cut(s) 150
BspPI GGATC 7 cut(s) 42, 84, 203, 222, 271, 309, 363
BspT107I GGYRCC 1 cut(s) 233
BsrBI CCGCTC 1 cut(s) 579
BsrGI TGTACA 1 cut(s) 113
BsrI ACTGG 2 cut(s) 185, 298
BssECI CCNNGG 6 cut(s) 133, 229, 351, 391, 443, 668
BssNI GRCGYC 1 cut(s) 234
BssT1I CCWWGG 3 cut(s) 351, 443, 668
Bst2UI CCWGG 2 cut(s) 135, 230
Bst4CI ACNGT 3 cut(s) 101, 373, 395
BstACI GRCGYC 1 cut(s) 234
BstAUI TGTACA 1 cut(s) 113
BstDEI CTNAG 3 cut(s) 48, 649, 687
BstDSI CCRYGG 1 cut(s) 391
BstF5I GGATG 6 cut(s) 61, 418, 481, 663, 669, 709
BstFNI CGCG 1 cut(s) 337
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 2 cut(s) 236, 337
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 4 cut(s) 27, 455, 504, 552
BstNI CCWGG 2 cut(s) 135, 230
BstSCI CCNGG 2 cut(s) 133, 228
BstSFI CTRYAG 1 cut(s) 146
BstUI CGCG 1 cut(s) 337
BstV1I GCAGC 2 cut(s) 132, 160
BstX2I RGATCY 4 cut(s) 34, 214, 263, 301
BstYI RGATCY 4 cut(s) 34, 214, 263, 301
Bsu15I ATCGAT 1 cut(s) 198
BsuRI GGCC 6 cut(s) 16, 132, 179, 522, 582, 675
BsuTUI ATCGAT 1 cut(s) 198
BtgI CCRYGG 1 cut(s) 391
BtgZI GCGATG 1 cut(s) 444
BtsCI GGATG 6 cut(s) 61, 418, 481, 663, 669, 709
BtsIMutI CAGTG 1 cut(s) 291
CfoI GCGC 2 cut(s) 236, 337
Cfr13I GGNCC 3 cut(s) 131, 178, 673
ClaI ATCGAT 1 cut(s) 198
CseI GACGC 1 cut(s) 140
Csp6I GTAC 2 cut(s) 114, 369
CviAII CATG 4 cut(s) 155, 359, 565, 584
CviQI GTAC 2 cut(s) 114, 369
DdeI CTNAG 3 cut(s) 48, 649, 687
DinI GGCGCC 1 cut(s) 235
EaeI YGGCCR 1 cut(s) 580
Ecl136II GAGCTC 2 cut(s) 21, 437
Eco130I CCWWGG 3 cut(s) 351, 443, 668
Eco24I GRGCYC 4 cut(s) 23, 439, 451, 637
Eco53kI GAGCTC 2 cut(s) 21, 437
Eco57I CTGAAG 3 cut(s) 203, 326, 545
EcoICRI GAGCTC 2 cut(s) 21, 437
EcoRII CCWGG 2 cut(s) 133, 228
EcoT14I CCWWGG 3 cut(s) 351, 443, 668
EcoT38I GRGCYC 4 cut(s) 23, 439, 451, 637
EgeI GGCGCC 1 cut(s) 235
EheI GGCGCC 1 cut(s) 235
ErhI CCWWGG 3 cut(s) 351, 443, 668
FaeI CATG 4 cut(s) 158, 362, 568, 587
FatI CATG 4 cut(s) 154, 358, 564, 583
FauNDI CATATG 1 cut(s) 678
FbaI TGATCA 2 cut(s) 322, 760
Fnu4HI GCNGC 5 cut(s) 146, 149, 403, 547, 580
FokI GGATG 6 cut(s) 68, 425, 488, 670, 676, 716
FriOI GRGCYC 4 cut(s) 23, 439, 451, 637
Fsp4HI GCNGC 5 cut(s) 146, 149, 403, 547, 580
FspBI CTAG 1 cut(s) 260
GlaI GCGC 2 cut(s) 235, 336
GluI GCNGC 5 cut(s) 146, 149, 403, 547, 580
GsaI CCCAGC 1 cut(s) 736
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 6 cut(s) 16, 132, 179, 522, 582, 675
HgaI GACGC 1 cut(s) 140
HhaI GCGC 2 cut(s) 236, 337
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 4 cut(s) 158, 362, 568, 587
Hin6I GCGC 2 cut(s) 234, 335
HinP1I GCGC 2 cut(s) 234, 335
HincII GTYRAC 1 cut(s) 751
HindII GTYRAC 1 cut(s) 751
HindIII AAGCTT 1 cut(s) 205
HinfI GANTC 2 cut(s) 63, 683
HphI GGTGA 6 cut(s) 113, 332, 397, 407, 473, 527
Hpy166II GTNNAC 2 cut(s) 388, 751
Hpy188I TCNGA 7 cut(s) 12, 90, 97, 241, 306, 616, 688
Hpy188III TCNNGA 3 cut(s) 254, 326, 655
Hpy8I GTNNAC 2 cut(s) 388, 751
Hpy99I CGWCG 4 cut(s) 101, 382, 554, 560
HpyAV CCTTC 3 cut(s) 281, 520, 694
HpyCH4III ACNGT 3 cut(s) 101, 373, 395
HpyCH4V TGCA 1 cut(s) 148
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 455, 504, 552
HpyF3I CTNAG 3 cut(s) 48, 649, 687
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 4 cut(s) 158, 362, 568, 587
HspAI GCGC 2 cut(s) 234, 335
KasI GGCGCC 1 cut(s) 233
Ksp22I TGATCA 2 cut(s) 322, 760
LmnI GCTCC 4 cut(s) 434, 442, 576, 592
Lsp1109I GCAGC 2 cut(s) 132, 160
LweI GCATC 1 cut(s) 654
MaeI CTAG 1 cut(s) 260
MaeIII GTNAC 1 cut(s) 395
MbiI CCGCTC 1 cut(s) 579
MboII GAAGA 2 cut(s) 209, 265
MflI RGATCY 4 cut(s) 34, 214, 263, 301
MhlI GDGCHC 4 cut(s) 23, 439, 451, 637
MluCI AATT 1 cut(s) 619
Mly113I GGCGCC 1 cut(s) 234
MlyI GAGTC 2 cut(s) 72, 677
MmeI TCCRAC 2 cut(s) 33, 120
MnlI CCTC 8 cut(s) 6, 205, 247, 311, 450, 536, 682, 710
MslI CAYNNNNRTG 2 cut(s) 296, 363
MspR9I CCNGG 2 cut(s) 135, 230
MvaI CCWGG 2 cut(s) 135, 230
MvnI CGCG 1 cut(s) 337
MwoI GCNNNNNNNGC 4 cut(s) 27, 455, 504, 552
NarI GGCGCC 1 cut(s) 234
NdeI CATATG 1 cut(s) 678
NlaIII CATG 4 cut(s) 158, 362, 568, 587
NlaIV GGNNCC 2 cut(s) 235, 674
NmeAIII GCCGAG 1 cut(s) 42
NmuCI GTSAC 1 cut(s) 395
PcsI WCGNNNNNNNCGW 1 cut(s) 558
PflMI CCANNNNNTGG 1 cut(s) 589
PkrI GCNGC 5 cut(s) 147, 150, 404, 548, 581
Ple19I CGATCG 1 cut(s) 244
PleI GAGTC 2 cut(s) 71, 677
PluTI GGCGCC 1 cut(s) 237
PpsI GAGTC 2 cut(s) 71, 677
Psp124BI GAGCTC 2 cut(s) 23, 439
Psp6I CCWGG 2 cut(s) 133, 228
PspFI CCCAGC 1 cut(s) 732
PspGI CCWGG 2 cut(s) 133, 228
PspN4I GGNNCC 2 cut(s) 235, 674
PspPI GGNCC 3 cut(s) 131, 178, 673
PstI CTGCAG 1 cut(s) 150
PsuI RGATCY 4 cut(s) 34, 214, 263, 301
PvuI CGATCG 1 cut(s) 244
RsaI GTAC 2 cut(s) 115, 370
RsaNI GTAC 2 cut(s) 114, 369
RseI CAYNNNNRTG 2 cut(s) 296, 363
SacI GAGCTC 2 cut(s) 23, 439
SatI GCNGC 5 cut(s) 146, 149, 403, 547, 580
Sau96I GGNCC 3 cut(s) 131, 178, 673
SchI GAGTC 2 cut(s) 72, 677
ScrFI CCNGG 2 cut(s) 135, 230
SduI GDGCHC 4 cut(s) 23, 439, 451, 637
SetI ASST 8 cut(s) 23, 209, 225, 322, 439, 531, 693, 721
SfaNI GCATC 1 cut(s) 654
SfcI CTRYAG 1 cut(s) 146
SfoI GGCGCC 1 cut(s) 235
SmiMI CAYNNNNRTG 2 cut(s) 296, 363
SmlI CTYRAG 1 cut(s) 636
SmoI CTYRAG 1 cut(s) 636
Sse9I AATT 1 cut(s) 619
SsiI CCGC 6 cut(s) 383, 402, 417, 546, 579, 708
SspDI GGCGCC 1 cut(s) 233
SspMI CTAG 1 cut(s) 260
SstI GAGCTC 2 cut(s) 23, 439
StyD4I CCNGG 2 cut(s) 133, 228
StyI CCWWGG 3 cut(s) 351, 443, 668
TaaI ACNGT 3 cut(s) 101, 373, 395
TaqI TCGA 2 cut(s) 198, 316
TasI AATT 1 cut(s) 619
TatI WGTACW 1 cut(s) 113
TauI GCSGC 3 cut(s) 405, 549, 582
TscAI CASTG 1 cut(s) 298
TseFI GTSAC 1 cut(s) 395
TseI GCWGC 2 cut(s) 145, 148
Tsp45I GTSAC 1 cut(s) 395
TspDTI ATGAA 2 cut(s) 581, 713
TspRI CASTG 1 cut(s) 298
Van91I CCANNNNNTGG 1 cut(s) 589
XapI RAATTY 1 cut(s) 619
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XspI CTAG 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.