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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 212 |
| AclWI | GGATC | 1 cut(s) | 182 |
| AluBI | AGCT | 1 cut(s) | 206 |
| AluI | AGCT | 1 cut(s) | 206 |
| AlwI | GGATC | 1 cut(s) | 182 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 191 |
| AoxI | GGCC | 1 cut(s) | 290 |
| BccI | CCATC | 1 cut(s) | 299 |
| Bse1I | ACTGG | 1 cut(s) | 125 |
| BseGI | GGATG | 1 cut(s) | 38 |
| BseMII | CTCAG | 1 cut(s) | 162 |
| BseNI | ACTGG | 1 cut(s) | 125 |
| BshFI | GGCC | 1 cut(s) | 292 |
| BslFI | GGGAC | 1 cut(s) | 290 |
| BsmFI | GGGAC | 1 cut(s) | 290 |
| BsnI | GGCC | 1 cut(s) | 292 |
| Bsp143I | GATC | 1 cut(s) | 187 |
| BspACI | CCGC | 1 cut(s) | 212 |
| BspANI | GGCC | 1 cut(s) | 292 |
| BspCNI | CTCAG | 1 cut(s) | 161 |
| BspPI | GGATC | 1 cut(s) | 182 |
| BsrI | ACTGG | 1 cut(s) | 125 |
| BssMI | GATC | 1 cut(s) | 187 |
| BstDEI | CTNAG | 1 cut(s) | 148 |
| BstF5I | GGATG | 1 cut(s) | 38 |
| BstKTI | GATC | 1 cut(s) | 190 |
| BstMBI | GATC | 1 cut(s) | 187 |
| BstX2I | RGATCY | 1 cut(s) | 187 |
| BstYI | RGATCY | 1 cut(s) | 187 |
| BsuRI | GGCC | 1 cut(s) | 292 |
| BtsCI | GGATG | 1 cut(s) | 38 |
| CaiI | CAGNNNCTG | 1 cut(s) | 191 |
| CviJI | RGCY | 4 cut(s) | 5, 206, 292, 314 |
| CviKI_1 | RGCY | 4 cut(s) | 5, 206, 292, 314 |
| DdeI | CTNAG | 1 cut(s) | 148 |
| DpnI | GATC | 1 cut(s) | 189 |
| DpnII | GATC | 1 cut(s) | 187 |
| Eco147I | AGGCCT | 1 cut(s) | 292 |
| FaiI | YATR | 2 cut(s) | 50, 119 |
| FaqI | GGGAC | 1 cut(s) | 290 |
| FauI | CCCGC | 1 cut(s) | 205 |
| FokI | GGATG | 1 cut(s) | 25 |
| HaeIII | GGCC | 1 cut(s) | 292 |
| HinfI | GANTC | 1 cut(s) | 194 |
| Hpy188III | TCNNGA | 3 cut(s) | 21, 185, 191 |
| HpyAV | CCTTC | 1 cut(s) | 54 |
| HpyCH4V | TGCA | 1 cut(s) | 71 |
| HpyF3I | CTNAG | 1 cut(s) | 148 |
| Kzo9I | GATC | 1 cut(s) | 187 |
| LmnI | GCTCC | 1 cut(s) | 303 |
| LpnPI | CCDG | 4 cut(s) | 6, 138, 198, 204 |
| MaeIII | GTNAC | 1 cut(s) | 64 |
| MalI | GATC | 1 cut(s) | 189 |
| MboI | GATC | 1 cut(s) | 187 |
| MflI | RGATCY | 1 cut(s) | 187 |
| MmeI | TCCRAC | 1 cut(s) | 185 |
| NdeII | GATC | 1 cut(s) | 187 |
| PceI | AGGCCT | 1 cut(s) | 292 |
| PfeI | GAWTC | 1 cut(s) | 194 |
| PstNI | CAGNNNCTG | 1 cut(s) | 191 |
| PsuI | RGATCY | 1 cut(s) | 187 |
| Sau3AI | GATC | 1 cut(s) | 187 |
| SetI | ASST | 2 cut(s) | 20, 208 |
| SseBI | AGGCCT | 1 cut(s) | 292 |
| SsiI | CCGC | 1 cut(s) | 212 |
| StuI | AGGCCT | 1 cut(s) | 292 |
| TaqI | TCGA | 1 cut(s) | 268 |
| TfiI | GAWTC | 1 cut(s) | 194 |
| TspDTI | ATGAA | 1 cut(s) | 27 |