Rroxscaffold_7G00190260

Non-structural maintenance of chromosomes element 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001336
Physical Location & Seq
Forward (+)
30318392 .. 30333435
15044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00190260.1

Sequence Viewer

Length: 1038 bp
ATGGCGGTCGATGTTGACGAACTAACCTTGCTTTTAAGATGCTTGTGTATCTTTGAGCATGGTGGACTCAAGTCTAGTAATGGAGAATACCCAATTTCTTCTTTCGGTTCTTTGGAAATTGATTGCTTTGGTTACAAGTGGATGCGTGCCCACGTGCCCCTGCGCACGCATCCCTGCTGCCCTGCAGCACCGCTGCACGTTTGCTGCCCCTGCAGCACCAACGCACACCCACTGCATCTTTTTCCCGTTCCTGTGCACATCTGTCTTCTTGAAAGCCCCGAAACGTCTAGTTCAGAACCTCAGATCAAAAAACTTTCTTCATCAAAGTTGTTCGTCCCTGTATTGTTTGGCTGGTTAAATAATGCGTTGGAGGTGTTTTCTCTAAATCATTCAGCTTCTAATTGTGGGCTTCATTGCTTCACAATATCTTTACAAATAGAAGCAATAGTGCAAGAAGCTGCTCAAGGAACCATATGTAGCAGTGACGCTCTTAATCTACGATTAGAGAATCAGGTTAACAGTGGTACATCATCACAATCACAAGGAGGTTCACTTCCTATCCCTACTGCATTGAAGAACTTTTCAATGTCCCAGAAGGAAAAAACTCTTGATGAATTTGTACGGGATAAATGGCTTTCCTTCATTGCTGAGGGTCATGTTGGACTTGGTGTCAGATCCTTCCTTGATCTGCGAAGTTGGTTTTGCAATAATGATGTTCCTTCATGCGAAGTGTGCAACGAAGCTGGCGTGAAGACGCCCATCAATCTCTCTCCCTCTGACGCTGCCTCATCTAGCCTTTCATCACGATTTTGGGTTGGAAATCGAAGCCTCACTCAAGCAAGGCTGCTTGGCGATTTGATTCAGATAGGTGACCTTGGAGTTCTTGGAACTGGAATACCAATAGGAAAACTTGGTATGTATGTTGCTGCAGCTGGTATTAATTCAGAGTCATTGGGAGTTCAAGCGGAATACCAAGCAACGCAGCAAGTTATATTATCTTTCATCATGCAAAACTTAGCTAGGAAATTTGAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SMC_Nse1 zf-RING-like

Protein Analysis

345

Amino Acids

37.37

Weight (kDa)

5.8

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMC_Nse1 PF07574 150 - 230 3.7e-10 Nse1 non-SMC component of SMC5-6 complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 164
AciI CCGC 3 cut(s) 5, 191, 965
AclWI GGATC 1 cut(s) 669
AcsI RAATTY 2 cut(s) 614, 1025
AcvI CACGTG 1 cut(s) 154
AcyI GRCGYC 1 cut(s) 755
AfaI GTAC 2 cut(s) 526, 621
AgsI TTSAA 5 cut(s) 272, 574, 585, 962, 1031
AhdI GACNNNNNGTC 1 cut(s) 668
AluBI AGCT 5 cut(s) 395, 458, 743, 932, 1019
AluI AGCT 5 cut(s) 395, 458, 743, 932, 1019
Alw21I GWGCWC 1 cut(s) 258
Alw44I GTGCAC 1 cut(s) 254
AlwI GGATC 1 cut(s) 669
ApaLI GTGCAC 1 cut(s) 254
ApoI RAATTY 2 cut(s) 614, 1025
AseI ATTAAT 1 cut(s) 939
AspLEI GCGC 1 cut(s) 165
AsuHPI GGTGA 1 cut(s) 881
BaeGI GKGCMC 3 cut(s) 151, 159, 258
BbrPI CACGTG 1 cut(s) 154
BbsI GAAGAC 2 cut(s) 257, 758
Bbv12I GWGCWC 1 cut(s) 258
BbvCI CCTCAGC 1 cut(s) 648
BccI CCATC 1 cut(s) 767
BfaI CTAG 4 cut(s) 75, 288, 792, 1020
BfmI CTRYAG 3 cut(s) 183, 211, 927
BmeRI GACNNNNNGTC 1 cut(s) 668
BmiI GGNNCC 1 cut(s) 469
BmsI GCATC 4 cut(s) 29, 132, 178, 244
BoxI GACNNNNGTC 1 cut(s) 70
BpiI GAAGAC 2 cut(s) 257, 758
Bpu10I CCTNAGC 1 cut(s) 648
BpuEI CTTGAG 3 cut(s) 53, 447, 819
BsaAI YACGTR 1 cut(s) 154
BsaHI GRCGYC 1 cut(s) 755
BsaJI CCNNGG 1 cut(s) 874
Bse1I ACTGG 1 cut(s) 895
Bse3DI GCAATG 2 cut(s) 412, 642
BseDI CCNNGG 1 cut(s) 874
BseGI GGATG 2 cut(s) 147, 169
BseMI GCAATG 2 cut(s) 412, 642
BseMII CTCAG 2 cut(s) 314, 639
BseNI ACTGG 1 cut(s) 895
BseSI GKGCMC 3 cut(s) 151, 159, 258
BsgI GTGCAG 1 cut(s) 179
Bsh1285I CGRYCG 1 cut(s) 9
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 258
BslFI GGGAC 2 cut(s) 320, 574
BsmFI GGGAC 2 cut(s) 320, 574
Bsp1286I GDGCHC 3 cut(s) 151, 159, 258
Bsp143I GATC 3 cut(s) 303, 674, 685
BspACI CCGC 3 cut(s) 5, 191, 965
BspCNI CTCAG 2 cut(s) 313, 640
BspLI GGNNCC 1 cut(s) 469
BspMAI CTGCAG 3 cut(s) 187, 215, 931
BspPI GGATC 1 cut(s) 669
BsrDI GCAATG 2 cut(s) 412, 642
BsrI ACTGG 1 cut(s) 895
BssECI CCNNGG 1 cut(s) 874
BssMI GATC 3 cut(s) 303, 674, 685
BssNI GRCGYC 1 cut(s) 755
BssT1I CCWWGG 1 cut(s) 874
Bst4CI ACNGT 1 cut(s) 521
BstACI GRCGYC 1 cut(s) 755
BstBAI YACGTR 1 cut(s) 154
BstC8I GCNNGC 3 cut(s) 147, 167, 745
BstDEI CTNAG 3 cut(s) 300, 648, 1015
BstEII GGTNACC 1 cut(s) 869
BstF5I GGATG 2 cut(s) 147, 169
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 3 cut(s) 306, 677, 688
BstMBI GATC 3 cut(s) 303, 674, 685
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 3 cut(s) 210, 213, 732
BstPAI GACNNNNGTC 1 cut(s) 70
BstPI GGTNACC 1 cut(s) 869
BstSFI CTRYAG 3 cut(s) 183, 211, 927
BstSLI GKGCMC 3 cut(s) 151, 159, 258
BstV2I GAAGAC 2 cut(s) 257, 758
BstX2I RGATCY 1 cut(s) 674
BstYI RGATCY 1 cut(s) 674
BtsCI GGATG 2 cut(s) 147, 169
BtsI GCAGTG 2 cut(s) 230, 487
BtsIMutI CAGTG 3 cut(s) 230, 487, 526
Cac8I GCNNGC 3 cut(s) 147, 167, 745
CfoI GCGC 1 cut(s) 165
CseI GACGC 3 cut(s) 494, 763, 788
Csp6I GTAC 2 cut(s) 525, 620
CviAII CATG 4 cut(s) 59, 656, 723, 1006
CviQI GTAC 2 cut(s) 525, 620
DdeI CTNAG 3 cut(s) 300, 648, 1015
DpnI GATC 3 cut(s) 305, 676, 687
DpnII GATC 3 cut(s) 303, 674, 685
DriI GACNNNNNGTC 1 cut(s) 668
Eam1105I GACNNNNNGTC 1 cut(s) 668
Eco130I CCWWGG 1 cut(s) 874
Eco72I CACGTG 1 cut(s) 154
Eco91I GGTNACC 1 cut(s) 869
EcoO65I GGTNACC 1 cut(s) 869
EcoT14I CCWWGG 1 cut(s) 874
ErhI CCWWGG 1 cut(s) 874
FaeI CATG 4 cut(s) 62, 659, 726, 1009
FaiI YATR 9 cut(s) 60, 473, 475, 657, 724, 917, 921, 992, 1007
FaqI GGGAC 2 cut(s) 320, 574
FatI CATG 4 cut(s) 58, 655, 722, 1005
FauNDI CATATG 1 cut(s) 473
FokI GGATG 2 cut(s) 154, 156
FspBI CTAG 4 cut(s) 75, 288, 792, 1020
FspI TGCGCA 1 cut(s) 164
GlaI GCGC 1 cut(s) 164
HgaI GACGC 3 cut(s) 494, 763, 788
HhaI GCGC 1 cut(s) 165
Hin1I GRCGYC 1 cut(s) 755
Hin1II CATG 4 cut(s) 62, 659, 726, 1009
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HincII GTYRAC 2 cut(s) 16, 517
HindII GTYRAC 2 cut(s) 16, 517
HinfI GANTC 4 cut(s) 66, 508, 859, 947
HpaI GTTAAC 1 cut(s) 517
HphI GGTGA 1 cut(s) 881
Hpy166II GTNNAC 5 cut(s) 16, 65, 256, 517, 551
Hpy188I TCNGA 6 cut(s) 295, 303, 674, 778, 864, 946
Hpy188III TCNNGA 3 cut(s) 269, 608, 804
Hpy8I GTNNAC 5 cut(s) 16, 65, 256, 517, 551
HpyAV CCTTC 4 cut(s) 589, 649, 688, 729
HpyCH4III ACNGT 1 cut(s) 521
HpyCH4IV ACGT 3 cut(s) 153, 198, 284
HpyF10VI GCNNNNNNNGC 3 cut(s) 210, 213, 732
HpyF3I CTNAG 3 cut(s) 300, 648, 1015
HpySE526I ACGT 3 cut(s) 153, 198, 284
Hsp92I GRCGYC 1 cut(s) 755
Hsp92II CATG 4 cut(s) 62, 659, 726, 1009
HspAI GCGC 1 cut(s) 163
KspAI GTTAAC 1 cut(s) 517
Kzo9I GATC 3 cut(s) 303, 674, 685
LweI GCATC 4 cut(s) 29, 132, 178, 244
MaeI CTAG 4 cut(s) 75, 288, 792, 1020
MaeII ACGT 3 cut(s) 153, 198, 284
MaeIII GTNAC 3 cut(s) 131, 482, 869
MalI GATC 3 cut(s) 305, 676, 687
MboI GATC 3 cut(s) 303, 674, 685
MboII GAAGA 5 cut(s) 90, 257, 309, 586, 763
MflI RGATCY 1 cut(s) 674
MhlI GDGCHC 3 cut(s) 151, 159, 258
MluCI AATT 7 cut(s) 93, 117, 400, 614, 940, 1025, 1033
MlyI GAGTC 2 cut(s) 60, 956
MmeI TCCRAC 3 cut(s) 348, 640, 796
MnlI CCTC 7 cut(s) 309, 364, 539, 643, 784, 796, 839
MseI TTAA 5 cut(s) 35, 356, 492, 516, 939
MspA1I CMGCKG 2 cut(s) 193, 932
MwoI GCNNNNNNNGC 3 cut(s) 210, 213, 732
NdeI CATATG 1 cut(s) 473
NdeII GATC 3 cut(s) 303, 674, 685
NlaIII CATG 4 cut(s) 62, 659, 726, 1009
NlaIV GGNNCC 1 cut(s) 469
NmuCI GTSAC 2 cut(s) 482, 869
NsbI TGCGCA 1 cut(s) 164
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 744
PfeI GAWTC 2 cut(s) 508, 859
PleI GAGTC 2 cut(s) 60, 955
PmaCI CACGTG 1 cut(s) 154
PmlI CACGTG 1 cut(s) 154
PpsI GAGTC 2 cut(s) 60, 955
Ppu21I YACGTR 1 cut(s) 154
PshAI GACNNNNGTC 1 cut(s) 70
PshBI ATTAAT 1 cut(s) 939
PspCI CACGTG 1 cut(s) 154
PspEI GGTNACC 1 cut(s) 869
PspN4I GGNNCC 1 cut(s) 469
PstI CTGCAG 3 cut(s) 187, 215, 931
PsuI RGATCY 1 cut(s) 674
PvuII CAGCTG 1 cut(s) 932
RsaI GTAC 2 cut(s) 526, 621
RsaNI GTAC 2 cut(s) 525, 620
SaqAI TTAA 5 cut(s) 35, 356, 492, 516, 939
Sau3AI GATC 3 cut(s) 303, 674, 685
SchI GAGTC 2 cut(s) 60, 956
SduI GDGCHC 3 cut(s) 151, 159, 258
SfaNI GCATC 4 cut(s) 29, 132, 178, 244
SfcI CTRYAG 3 cut(s) 183, 211, 927
SmlI CTYRAG 3 cut(s) 68, 462, 834
SmoI CTYRAG 3 cut(s) 68, 462, 834
Sse9I AATT 7 cut(s) 93, 117, 400, 614, 940, 1025, 1033
SsiI CCGC 3 cut(s) 5, 191, 965
SspMI CTAG 4 cut(s) 75, 288, 792, 1020
StyI CCWWGG 1 cut(s) 874
TaaI ACNGT 1 cut(s) 521
TaiI ACGT 3 cut(s) 156, 201, 287
TaqI TCGA 2 cut(s) 9, 823
TasI AATT 7 cut(s) 93, 117, 400, 614, 940, 1025, 1033
TfiI GAWTC 2 cut(s) 508, 859
Tru1I TTAA 5 cut(s) 35, 356, 492, 516, 939
Tru9I TTAA 5 cut(s) 35, 356, 492, 516, 939
TscAI CASTG 3 cut(s) 237, 487, 526
TseFI GTSAC 2 cut(s) 482, 869
Tsp45I GTSAC 2 cut(s) 482, 869
TspDTI ATGAA 7 cut(s) 309, 401, 627, 631, 711, 789, 991
TspRI CASTG 3 cut(s) 237, 487, 526
VneI GTGCAC 1 cut(s) 254
VspI ATTAAT 1 cut(s) 939
XapI RAATTY 2 cut(s) 614, 1025
XspI CTAG 4 cut(s) 75, 288, 792, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.