AT1G06900

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000891
Physical Location & Seq
Reverse (-)
2114765 .. 2120745
5981 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G06900.1

Sequence Viewer

Length: 3075 bp
ATGTCTTCAATGAAATCCGTCTCGGCTTTGGACAATGTAGTGGTGAAGTCACCAAACGATCGGAGGTTATACAGAGTGATTGAATTGGAGAATGGATTGTGTGCTCTGCTCATTCACGATCCAGATATTTACCCCGAAGGCTCTGTTCCGGATCAAATTGATGAAGATGACGAGGACGGTGAAGAAGAAGATAGTGACGGGAGCTCTGAAGATGATGATGATGATGAAGATGATGAGGAAGATGGGGAAGGAGATGAAGAAGATGAGGATGAGGATGAAGATGAAGTGAAAGGGAAAGGGGATCATCAGACCAAAAAGGCTGCTGCAGCTATGTGTGTTTCAATGGGAAGCTTCTTGGATCCTCCGGAGGCACAAGGCCTGGCTCACTTTCTTGAACACATGCTTTTTATGGGTAGCACTGAGTTCCCCGATGAGAATGAATATGATAGTTACTTGTCTAAGCATGGTGGATCCTCTAACGCATACACAGAAATGGAGCATACATGCTATCACTTCGAAGTCAAGAGAGAATTTCTTCAAGGGGCCTTGAAAAGATTCTCTCAGTTTTTTGTTGCACCGCTCATGAAGACTGAAGCTATGGAACGGGAAGTCCTGGCCGTTGATTCAGAATTTAACCAGGCCCTCCAAAATGATGCATGTCGCCTGCAACAACTTCAGTGCTATACATCTGCAAAGGGTCATCCTTTTAATAGGTTTGCATGGGGTAACAAGAAGAGCTTAAGTGGTGCAATGGAAAATGGGGTTGATCTACGGGAATGTATTGTGAAATTATACAAGGAATATTACCATGGTGGACTGATGAAGCTCGTTGTCATTGGGGGAGAATCTCTCGACATGCTTGAAAGTTGGGTTGTAGAACTATTTGGTGATGTCAAAAACGGATCCAAAATCAGGCCAACTCTGGAGGCAGAAGGTCCTATTTGGAAAGGCGGTAAGTTATATCGCCTAGAGGCGGTTAAAGATGTTCATATACTTGATTTGACATGGACTCTCCCTCCTCTTCGCTCTGCCTATGTGAAGAAGCCGGAAGACTACCTAGCACATCTATTAGGACATGAGGGTAGAGGAAGTCTGCATTCATTCCTCAAAGCCAAGGGTTGGGCAACCTCACTGTCTGCTGGTGTTGGGGATGATGGAATCAACCGCTCGTCTCTAGCATATGTGTTCGGCATGTCCATACATCTCACTGACTCTGGTTTAGAGAAGATTTATGACATCATTGGTTACATCTATCAATATCTCAAGTTATTGCGTGATGTGTCACCACAAGAATGGATATTCAAGGAACTCCAAGATATTGGGAACATGGACTTCAGATTTGCTGAGGAGCAGCCGGCAGATGATTATGCTGCTGAGCTCTCAGAGAATATGCTTGCTTATCCAGTGGAGCACGTTATTTATGGTGATTATGTGTACCAGACATGGGATCCAAAATTGATAGAAGATCTTATGGGTTTTTTCACACCTCAGAACATGAGGATTGATGTTGTTTCGAAATCCATCAAGTCGGAAGAGTTCCAACAAGAACCTTGGTTCGGTTCTAGTTACATAGAGGAAGATGTTCCATTGTCTTTGATGGAATCATGGAGCAATCCTTCAGAAGTAGATAACTCTTTACATCTCCCCTCGAAAAACCAATTCATCCCTTGTGATTTTTCGATCCGAGCCATTAACTCTGATGTGGATCCCAAAAGTCAGTCTCCTCCGAGATGTATAATTGATGAACCGTTCATGAAATTCTGGTACAAGCTTGACGAAACTTTTAAGGTTCCTCGTGCAAATACATACTTCCGCATAAATTTGAAGGGGGCATATGCCAGTGTGAAGAACTGCCTTTTGACAGAATTATATATTAACCTTCTGAAAGATGAGCTTAATGAGATCATCTACCAGGCCAGTATAGCAAAACTTGAAACTTCTTTATCTATGTATGGTGATAAGCTAGAGCTTAAAGTGTATGGCTTTAATGAGAAAATTCCAGCTCTTCTATCAAAAATCTTAGCCATAGCCAAATCCTTCATGCCAAACCTGGAGCGATTTAAGGTTATCAAAGAAAACATGGAGAGAGGGTTTAGGAATACTAATATGAAGCCTTTGAACCATTCTACATACTTGAGACTGCAACTCTTGTGTAAACGGATCTATGACAGCGATGAAAAGTTGTCTGTACTAAATGATTTGTCTCTCGATGATCTCAACAGCTTTATTCCTGAACTCCGCTCTCAGATATTTATTGAGGCTCTATGTCATGGTAATTTGTCCGAAGACGAAGCAGTGAACATATCAAACATTTTCAAAGACAGTTTGACGGTTGAACCACTCCCAAGCAAATGTAGACATGGTGAACAGATAACATGTTTCCCTATGGGTGCCAAACTTGTGAGAGATGTCAATGTGAAGAACAAATCTGAAACAAACTCAGTAGTCGAGCTTTACTATCAAATCGAGCCTGAAGAAGCTCAATCAACGAGAACGAAAGCTGTGCTGGATCTCTTTCATGAAATCATAGAAGAGCCATTGTTCAATCAGTTGAGGACAAAGGAGCAGCTTGGTTATGTTGTCGAGTGTGGCCCTCGCTTAACGTATCGTGTGCACGGTTTCTGTTTCTGTGTTCAATCTTCTAAGTACGGTCCAGTTCATTTGCTGGGGAGAGTTGACAATTTCATAAAAGATATCGAAGGGCTTCTGGAACAACTGGATGATGAATCCTATGAAGATTACCGAAGTGGTATGATTGCTAGATTGCTGGAAAAGGATCCCTCTCTCTTGTCCGAGACAAATGACTTATGGAGTCAGATTGTTGACAAAAGGTACATGTTTGATTTCTCCCACAAAGAAGCAGAAGAACTAAGAAGTATACAGAAGAAAGATGTGATCAGTTGGTACAAAACCTATTTCAGAGAATCATCACCCAAATGTCGTAGGCTTGCGGTAAGGGTTTGGGGATGCGATACCAATATGAAAGAAACTCAAACAGATCAAAAGGCGGTGCAGGTCATCGCAGACGCAGTGGCTTTCAAGTCAACTTCTAAGTTTTACCCCAGCCTTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

1024

Amino Acids

117.17

Weight (kDa)

4.77

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 101 - 224 1.3e-33 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 261 - 438 2.9e-20 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 445 - 729 4.5e-90 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 733 - 917 1.3e-18 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 838 - 937 2.3e-25 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 3004
AccB1I GGYRCC 1 cut(s) 2390
AccB7I CCANNNNNTGG 1 cut(s) 1119
AccBSI CCGCTC 3 cut(s) 580, 1167, 2241
AccI GTMKAC 2 cut(s) 2356, 2878
AccIII TCCGGA 2 cut(s) 148, 364
AciI CCGC 8 cut(s) 578, 951, 974, 1165, 1813, 2239, 2951, 3008
AcoI YGGCCR 1 cut(s) 615
AcsI RAATTY 5 cut(s) 530, 629, 1757, 1819, 1995
AcuI CTGAAG 6 cut(s) 228, 612, 659, 1318, 1602, 2493
AfaI GTAC 6 cut(s) 1436, 1766, 2190, 2648, 2834, 2906
AfiI CCNNNNNNNGG 5 cut(s) 912, 973, 1119, 1444, 1556
AflII CTTAAG 1 cut(s) 739
AflIII ACRYGT 2 cut(s) 2375, 2835
AjnI CCWGG 5 cut(s) 378, 612, 636, 1911, 2049
AloI GAACNNNNNNTCC 2 cut(s) 594, 626
Alw21I GWGCWC 5 cut(s) 106, 206, 1380, 1413, 2616
Alw26I GTCTC 6 cut(s) 25, 1176, 1725, 2131, 2208, 2789
Alw44I GTGCAC 1 cut(s) 2612
Aor13HI TCCGGA 2 cut(s) 148, 364
AoxI GGCC 7 cut(s) 376, 543, 615, 639, 914, 1914, 2590
ApaLI GTGCAC 1 cut(s) 2612
ApeKI GCWGC 6 cut(s) 320, 323, 326, 1351, 1370, 2566
ApoI RAATTY 5 cut(s) 530, 629, 1757, 1819, 1995
ArsI GACNNNNNNTTYG 2 cut(s) 1322, 1354
Asp700I GAANNNNTTC 4 cut(s) 534, 554, 1581, 2703
AspS9I GGNCC 5 cut(s) 543, 640, 935, 2591, 2651
AsuHPI GGTGA 9 cut(s) 42, 55, 191, 899, 1275, 1436, 1967, 2375, 2922
AsuII TTCGAA 2 cut(s) 516, 1514
AvaII GGWCC 2 cut(s) 935, 2651
BaeGI GKGCMC 1 cut(s) 2616
BamHI GGATCC 6 cut(s) 358, 470, 902, 1447, 1705, 2776
BanI GGYRCC 1 cut(s) 2390
BanII GRGCYC 2 cut(s) 206, 1380
BauI CACGAG 1 cut(s) 1794
BbsI GAAGAC 3 cut(s) 593, 1056, 2292
Bbv12I GWGCWC 5 cut(s) 106, 206, 1380, 1413, 2616
BbvCI CCTCAGC 1 cut(s) 1344
BbvI GCAGC 6 cut(s) 307, 310, 338, 1357, 1363, 2578
BccI CCATC 4 cut(s) 236, 1148, 1529, 1591
BceAI ACGGC 1 cut(s) 602
BcgI CGANNNNNNTGC 2 cut(s) 2485, 2519
BciT130I CCWGG 5 cut(s) 380, 614, 638, 1913, 2051
BclI TGATCA 1 cut(s) 2895
BcoDI GTCTC 6 cut(s) 25, 1176, 1725, 2131, 2208, 2789
BfaI CTAG 6 cut(s) 968, 1058, 1175, 1563, 1964, 2760
BfmI CTRYAG 1 cut(s) 324
BfrI CTTAAG 1 cut(s) 739
BfuAI ACCTGC 1 cut(s) 3004
BglII AGATCT 1 cut(s) 1465
BisI GCNGC 6 cut(s) 321, 324, 327, 1352, 1371, 2567
BlpI GCTNAGC 1 cut(s) 1374
BlsI GCNGC 6 cut(s) 322, 325, 328, 1353, 1372, 2568
Bme1390I CCNGG 5 cut(s) 380, 614, 638, 1913, 2051
Bme18I GGWCC 2 cut(s) 935, 2651
BmgT120I GGNCC 5 cut(s) 543, 640, 935, 2591, 2651
BmiI GGNNCC 9 cut(s) 360, 472, 544, 904, 1449, 1707, 1791, 2392, 2778
BmrFI CCNGG 5 cut(s) 380, 614, 638, 1913, 2051
BmsI GCATC 2 cut(s) 643, 2957
BpiI GAAGAC 3 cut(s) 593, 1056, 2292
BplI GAGNNNNNCTC 2 cut(s) 834, 866
BpmI CTGGAG 2 cut(s) 944, 2072
Bpu10I CCTNAGC 1 cut(s) 1344
Bpu1102I GCTNAGC 1 cut(s) 1374
Bpu14I TTCGAA 2 cut(s) 516, 1514
BpuEI CTTGAG 2 cut(s) 1247, 2155
BsaBI GATNNNNATC 1 cut(s) 1704
BsaJI CCNNGG 3 cut(s) 808, 1113, 1550
BsaWI WCCGGW 2 cut(s) 148, 364
BsaXI ACNNNNNCTCC 4 cut(s) 1000, 1030, 2075, 2105
Bsc4I CCNNNNNNNGG 5 cut(s) 912, 973, 1119, 1444, 1556
Bse118I RCCGGY 1 cut(s) 1354
Bse1I ACTGG 5 cut(s) 1403, 1839, 1917, 2654, 2721
Bse3DI GCAATG 1 cut(s) 756
Bse8I GATNNNNATC 1 cut(s) 1704
BseAI TCCGGA 2 cut(s) 148, 364
BseBI CCWGG 5 cut(s) 380, 614, 638, 1913, 2051
BseDI CCNNGG 3 cut(s) 808, 1113, 1550
BseGI GGATG 7 cut(s) 274, 280, 700, 1156, 1662, 2725, 2972
BseJI GATNNNNATC 1 cut(s) 1704
BseLI CCNNNNNNNGG 5 cut(s) 912, 973, 1119, 1444, 1556
BseMI GCAATG 1 cut(s) 756
BseMII CTCAG 8 cut(s) 411, 575, 1335, 1365, 1395, 1502, 2258, 2454
BseNI ACTGG 5 cut(s) 1403, 1839, 1917, 2654, 2721
BseRI GAGGAG 3 cut(s) 1008, 1361, 1713
BseSI GKGCMC 1 cut(s) 2616
BseXI GCAGC 6 cut(s) 307, 310, 338, 1357, 1363, 2578
BseYI CCCAGC 2 cut(s) 2665, 3062
BsgI GTGCAG 1 cut(s) 3032
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 7 cut(s) 378, 545, 617, 641, 916, 1916, 2592
BshNI GGYRCC 1 cut(s) 2390
BsiEI CGRYCG 1 cut(s) 61
BsiHKAI GWGCWC 5 cut(s) 106, 206, 1380, 1413, 2616
BsiSI CCGG 4 cut(s) 149, 365, 1046, 1355
BslI CCNNNNNNNGG 5 cut(s) 912, 973, 1119, 1444, 1556
BsmAI GTCTC 6 cut(s) 25, 1176, 1725, 2131, 2208, 2789
BsmBI CGTCTC 2 cut(s) 25, 1176
BsmI GAATGC 1 cut(s) 1096
BsnI GGCC 7 cut(s) 378, 545, 617, 641, 916, 1916, 2592
Bsp119I TTCGAA 2 cut(s) 516, 1514
Bsp1286I GDGCHC 5 cut(s) 106, 206, 1380, 1413, 2616
Bsp13I TCCGGA 2 cut(s) 148, 364
Bsp1720I GCTNAGC 1 cut(s) 1374
Bsp19I CCATGG 1 cut(s) 808
BspACI CCGC 8 cut(s) 578, 951, 974, 1165, 1813, 2239, 2951, 3008
BspANI GGCC 7 cut(s) 378, 545, 617, 641, 916, 1916, 2592
BspCNI CTCAG 8 cut(s) 412, 574, 1336, 1366, 1394, 1501, 2257, 2453
BspEI TCCGGA 2 cut(s) 148, 364
BspHI TCATGA 3 cut(s) 582, 1752, 2518
BspLI GGNNCC 9 cut(s) 360, 472, 544, 904, 1449, 1707, 1791, 2392, 2778
BspMAI CTGCAG 1 cut(s) 328
BspMI ACCTGC 1 cut(s) 3004
BspQI GCTCTTC 3 cut(s) 728, 2010, 2526
BspT104I TTCGAA 2 cut(s) 516, 1514
BspT107I GGYRCC 1 cut(s) 2390
BspTI CTTAAG 1 cut(s) 739
BsrBI CCGCTC 3 cut(s) 580, 1167, 2241
BsrDI GCAATG 1 cut(s) 756
BsrFI RCCGGY 1 cut(s) 1354
BsrI ACTGG 5 cut(s) 1403, 1839, 1917, 2654, 2721
BssAI RCCGGY 1 cut(s) 1354
BssECI CCNNGG 3 cut(s) 808, 1113, 1550
BssNAI GTATAC 1 cut(s) 2879
BssSI CACGAG 1 cut(s) 1794
BssT1I CCWWGG 3 cut(s) 808, 1113, 1550
Bst1107I GTATAC 1 cut(s) 2879
Bst2BI CACGAG 1 cut(s) 1794
Bst2UI CCWGG 5 cut(s) 380, 614, 638, 1913, 2051
Bst4CI ACNGT 7 cut(s) 179, 1134, 1749, 2324, 2332, 2618, 2651
Bst6I CTCTTC 5 cut(s) 728, 1026, 1527, 2010, 2526
BstAFI CTTAAG 1 cut(s) 739
BstBI TTCGAA 2 cut(s) 516, 1514
BstC8I GCNNGC 4 cut(s) 665, 1356, 1395, 2949
BstDSI CCRYGG 1 cut(s) 808
BstF5I GGATG 7 cut(s) 274, 280, 700, 1156, 1662, 2725, 2972
BstMAI GTCTC 6 cut(s) 25, 1176, 1725, 2131, 2208, 2789
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 2 cut(s) 326, 1922
BstNI CCWGG 5 cut(s) 380, 614, 638, 1913, 2051
BstNSI RCATGY 7 cut(s) 403, 507, 660, 859, 1195, 2379, 2839
BstSCI CCNGG 5 cut(s) 378, 612, 636, 1911, 2049
BstSFI CTRYAG 1 cut(s) 324
BstSLI GKGCMC 1 cut(s) 2616
BstV1I GCAGC 6 cut(s) 307, 310, 338, 1357, 1363, 2578
BstV2I GAAGAC 3 cut(s) 593, 1056, 2292
BstX2I RGATCY 9 cut(s) 358, 470, 902, 1447, 1465, 1705, 2160, 2509, 2776
BstXI CCANNNNNNTGG 2 cut(s) 1293, 1319
BstYI RGATCY 9 cut(s) 358, 470, 902, 1447, 1465, 1705, 2160, 2509, 2776
BstZ17I GTATAC 1 cut(s) 2879
BsuRI GGCC 7 cut(s) 378, 545, 617, 641, 916, 1916, 2592
BtgI CCRYGG 1 cut(s) 808
BtgZI GCGATG 2 cut(s) 2187, 3004
BtsCI GGATG 7 cut(s) 274, 280, 700, 1156, 1662, 2725, 2972
BtsI GCAGTG 2 cut(s) 2301, 3036
BtsIMutI CAGTG 8 cut(s) 417, 683, 1130, 1206, 1410, 1846, 2301, 3036
BveI ACCTGC 1 cut(s) 3004
Cac8I GCNNGC 4 cut(s) 665, 1356, 1395, 2949
CciI TCATGA 3 cut(s) 582, 1752, 2518
Cfr10I RCCGGY 1 cut(s) 1354
Cfr13I GGNCC 5 cut(s) 543, 640, 935, 2591, 2651
CseI GACGC 1 cut(s) 3035
Csp6I GTAC 6 cut(s) 1435, 1765, 2189, 2647, 2833, 2905
CviQI GTAC 6 cut(s) 1435, 1765, 2189, 2647, 2833, 2905
EaeI YGGCCR 1 cut(s) 615
Eam1104I CTCTTC 5 cut(s) 728, 1026, 1527, 2010, 2526
EarI CTCTTC 5 cut(s) 728, 1026, 1527, 2010, 2526
Ecl136II GAGCTC 2 cut(s) 204, 1378
Eco130I CCWWGG 3 cut(s) 808, 1113, 1550
Eco147I AGGCCT 1 cut(s) 378
Eco24I GRGCYC 2 cut(s) 206, 1380
Eco32I GATATC 1 cut(s) 2695
Eco47I GGWCC 2 cut(s) 935, 2651
Eco53kI GAGCTC 2 cut(s) 204, 1378
Eco57I CTGAAG 6 cut(s) 228, 612, 659, 1318, 1602, 2493
EcoICRI GAGCTC 2 cut(s) 204, 1378
EcoO109I RGGNCCY 3 cut(s) 543, 640, 935
EcoRII CCWGG 5 cut(s) 378, 612, 636, 1911, 2049
EcoRV GATATC 1 cut(s) 2695
EcoT14I CCWWGG 3 cut(s) 808, 1113, 1550
EcoT22I ATGCAT 1 cut(s) 658
EcoT38I GRGCYC 2 cut(s) 206, 1380
ErhI CCWWGG 3 cut(s) 808, 1113, 1550
Esp3I CGTCTC 2 cut(s) 25, 1176
FalI AAGNNNNNCTT 4 cut(s) 722, 754, 1878, 1910
FauNDI CATATG 2 cut(s) 1180, 1834
FbaI TGATCA 1 cut(s) 2895
FblI GTMKAC 2 cut(s) 2356, 2878
Fnu4HI GCNGC 6 cut(s) 321, 324, 327, 1352, 1371, 2567
FokI GGATG 7 cut(s) 281, 287, 687, 1163, 1649, 2732, 2979
FriOI GRGCYC 2 cut(s) 206, 1380
Fsp4HI GCNGC 6 cut(s) 321, 324, 327, 1352, 1371, 2567
FspBI CTAG 6 cut(s) 968, 1058, 1175, 1563, 1964, 2760
GluI GCNGC 6 cut(s) 321, 324, 327, 1352, 1371, 2567
GsaI CCCAGC 2 cut(s) 2669, 3066
GsuI CTGGAG 2 cut(s) 944, 2072
HaeIII GGCC 7 cut(s) 378, 545, 617, 641, 916, 1916, 2592
HapII CCGG 4 cut(s) 149, 365, 1046, 1355
HgaI GACGC 1 cut(s) 3035
HincII GTYRAC 3 cut(s) 2677, 2824, 3045
HindII GTYRAC 3 cut(s) 2677, 2824, 3045
HindIII AAGCTT 2 cut(s) 349, 1769
HpaII CCGG 4 cut(s) 149, 365, 1046, 1355
HphI GGTGA 9 cut(s) 42, 55, 191, 899, 1275, 1436, 1967, 2375, 2922
HpyAV CCTTC 8 cut(s) 131, 242, 926, 1626, 1819, 1889, 2047, 2693
HpyCH4III ACNGT 7 cut(s) 179, 1134, 1749, 2324, 2332, 2618, 2651
HpyCH4IV ACGT 2 cut(s) 1413, 2603
HpyF10VI GCNNNNNNNGC 2 cut(s) 326, 1922
HpySE526I ACGT 2 cut(s) 1413, 2603
Kpn2I TCCGGA 2 cut(s) 148, 364
KroI GCCGGC 1 cut(s) 1354
KroNI GCCGGC 1 cut(s) 1356
Ksp22I TGATCA 1 cut(s) 2895
LguI GCTCTTC 3 cut(s) 728, 2010, 2526
LmnI GCTCC 7 cut(s) 201, 496, 1348, 1408, 1608, 2053, 2563
Lsp1109I GCAGC 6 cut(s) 307, 310, 338, 1357, 1363, 2578
LweI GCATC 2 cut(s) 643, 2957
MaeI CTAG 6 cut(s) 968, 1058, 1175, 1563, 1964, 2760
MaeII ACGT 2 cut(s) 1413, 2603
MaeIII GTNAC 7 cut(s) 48, 194, 449, 725, 1244, 1281, 1565
MbiI CCGCTC 3 cut(s) 580, 1167, 2241
MflI RGATCY 9 cut(s) 358, 470, 902, 1447, 1465, 1705, 2160, 2509, 2776
MhlI GDGCHC 5 cut(s) 106, 206, 1380, 1413, 2616
MlyI GAGTC 3 cut(s) 1003, 1205, 2821
MmeI TCCRAC 2 cut(s) 1509, 1564
Mph1103I ATGCAT 1 cut(s) 658
MroI TCCGGA 2 cut(s) 148, 364
MroNI GCCGGC 1 cut(s) 1354
MroXI GAANNNNTTC 4 cut(s) 534, 554, 1581, 2703
MslI CAYNNNNRTG 3 cut(s) 491, 1291, 2935
MspCI CTTAAG 1 cut(s) 739
MspI CCGG 4 cut(s) 149, 365, 1046, 1355
MspR9I CCNGG 5 cut(s) 380, 614, 638, 1913, 2051
Mva1269I GAATGC 1 cut(s) 1096
MvaI CCWGG 5 cut(s) 380, 614, 638, 1913, 2051
MwoI GCNNNNNNNGC 2 cut(s) 326, 1922
NaeI GCCGGC 1 cut(s) 1356
NcoI CCATGG 1 cut(s) 808
NdeI CATATG 2 cut(s) 1180, 1834
NgoMIV GCCGGC 1 cut(s) 1354
NlaIV GGNNCC 9 cut(s) 360, 472, 544, 904, 1449, 1707, 1791, 2392, 2778
NmuCI GTSAC 3 cut(s) 48, 194, 1281
NsiI ATGCAT 1 cut(s) 658
NspI RCATGY 7 cut(s) 403, 507, 660, 859, 1195, 2379, 2839
NspV TTCGAA 2 cut(s) 516, 1514
PagI TCATGA 3 cut(s) 582, 1752, 2518
PceI AGGCCT 1 cut(s) 378
PciI ACATGT 2 cut(s) 2375, 2835
PciSI GCTCTTC 3 cut(s) 728, 2010, 2526
PctI GAATGC 1 cut(s) 1096
PdiI GCCGGC 1 cut(s) 1356
PdmI GAANNNNTTC 4 cut(s) 534, 554, 1581, 2703
PfeI GAWTC 7 cut(s) 555, 623, 845, 1158, 1601, 2726, 2924
PflMI CCANNNNNTGG 1 cut(s) 1119
PkrI GCNGC 6 cut(s) 322, 325, 328, 1353, 1372, 2568
Ple19I CGATCG 1 cut(s) 61
PleI GAGTC 3 cut(s) 1003, 1205, 2820
PpsI GAGTC 3 cut(s) 1003, 1205, 2820
PpuMI RGGWCCY 1 cut(s) 935
PscI ACATGT 2 cut(s) 2375, 2835
Psp124BI GAGCTC 2 cut(s) 206, 1380
Psp5II RGGWCCY 1 cut(s) 935
Psp6I CCWGG 5 cut(s) 378, 612, 636, 1911, 2049
PspFI CCCAGC 2 cut(s) 2665, 3062
PspGI CCWGG 5 cut(s) 378, 612, 636, 1911, 2049
PspN4I GGNNCC 9 cut(s) 360, 472, 544, 904, 1449, 1707, 1791, 2392, 2778
PspPI GGNCC 5 cut(s) 543, 640, 935, 2591, 2651
PspPPI RGGWCCY 1 cut(s) 935
PstI CTGCAG 1 cut(s) 328
PsuI RGATCY 9 cut(s) 358, 470, 902, 1447, 1465, 1705, 2160, 2509, 2776
PvuI CGATCG 1 cut(s) 61
RsaI GTAC 6 cut(s) 1436, 1766, 2190, 2648, 2834, 2906
RsaNI GTAC 6 cut(s) 1435, 1765, 2189, 2647, 2833, 2905
RseI CAYNNNNRTG 3 cut(s) 491, 1291, 2935
SacI GAGCTC 2 cut(s) 206, 1380
SapI GCTCTTC 3 cut(s) 728, 2010, 2526
SatI GCNGC 6 cut(s) 321, 324, 327, 1352, 1371, 2567
Sau96I GGNCC 5 cut(s) 543, 640, 935, 2591, 2651
SchI GAGTC 3 cut(s) 1003, 1205, 2821
ScrFI CCNGG 5 cut(s) 380, 614, 638, 1913, 2051
SduI GDGCHC 5 cut(s) 106, 206, 1380, 1413, 2616
SfaNI GCATC 2 cut(s) 643, 2957
SfcI CTRYAG 1 cut(s) 324
SfuI TTCGAA 2 cut(s) 516, 1514
SinI GGWCC 2 cut(s) 935, 2651
SmiMI CAYNNNNRTG 3 cut(s) 491, 1291, 2935
SmlI CTYRAG 3 cut(s) 739, 1262, 2134
SmoI CTYRAG 3 cut(s) 739, 1262, 2134
SseBI AGGCCT 1 cut(s) 378
SsiI CCGC 8 cut(s) 578, 951, 974, 1165, 1813, 2239, 2951, 3008
SspI AATATT 1 cut(s) 803
SspMI CTAG 6 cut(s) 968, 1058, 1175, 1563, 1964, 2760
SstI GAGCTC 2 cut(s) 206, 1380
StuI AGGCCT 1 cut(s) 378
StyD4I CCNGG 5 cut(s) 378, 612, 636, 1911, 2049
StyI CCWWGG 3 cut(s) 808, 1113, 1550
TaaI ACNGT 7 cut(s) 179, 1134, 1749, 2324, 2332, 2618, 2651
TaiI ACGT 2 cut(s) 1416, 2606
TatI WGTACW 1 cut(s) 2188
TfiI GAWTC 7 cut(s) 555, 623, 845, 1158, 1601, 2726, 2924
TscAI CASTG 8 cut(s) 424, 683, 1137, 1213, 1410, 1846, 2301, 3036
TseFI GTSAC 3 cut(s) 48, 194, 1281
TseI GCWGC 6 cut(s) 320, 323, 326, 1351, 1370, 2566
Tsp45I GTSAC 3 cut(s) 48, 194, 1281
TspGWI ACGGA 3 cut(s) 7, 915, 2173
TspRI CASTG 8 cut(s) 424, 683, 1137, 1213, 1410, 1846, 2301, 3036
Van91I CCANNNNNTGG 1 cut(s) 1119
Vha464I CTTAAG 1 cut(s) 739
VneI GTGCAC 1 cut(s) 2612
VpaK11BI GGWCC 2 cut(s) 935, 2651
XapI RAATTY 5 cut(s) 530, 629, 1757, 1819, 1995
XceI RCATGY 7 cut(s) 403, 507, 660, 859, 1195, 2379, 2839
XmiI GTMKAC 2 cut(s) 2356, 2878
XmnI GAANNNNTTC 4 cut(s) 534, 554, 1581, 2703
XspI CTAG 6 cut(s) 968, 1058, 1175, 1563, 1964, 2760
Zsp2I ATGCAT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.