MD03G1052300.v1.1

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000891
Physical Location & Seq
Forward (+)
4166527 .. 4175409
8883 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1052300.v1.1.491

Sequence Viewer

Length: 3090 bp
ATGGCTCGCTGCACTTTCAAATCGGACGATATCATAATAAAGTCGCCGAATGACAGACGATTATACAGATTAATCAAGCTCGAGAATAGCCTCTCGGCATTGCTCGTTCATGATCCTGAGATTTACCCAGAAGGGCCGCCTGATCACTCCAAATCCGTCGAACACAGTGAGGCTGAAGAGGAAGAAGAGGACGAAGATATGGACGACGAGGACGGAGATGAGGGGGAAGACAGTGAAGGAGATGAGGATGAAGAGGACGATGACGAAGAAGGCGGAGATGGTGAACTGAAGAAGAAGGGGAAGGGTGGAGACTCTCAGACTAAGAAGGCAGCAGCAGCAATGTGTGTGGGAATAGGCAGCTTCTCCGACCCTTTTGAGGCACAAGGGCTTGCACACTTTCTAGAACACATGCTCTTCATGGGGAGTACAGAGTTTCCAGATGAAAATGAGTATGATAGTTACTTGTCCAAGCATGGAGGTTCATCAAATGCATACACAGAAGCAGAGCATACCTGCTACCATTTTGAAGTGAAGCCAGAGTTTCTTAAGGGTGCCTTGAGAAGATTTTCTCAGTTCTTTGTTTCACCTCTAGTGAAAATTGAAGCGATGGAGCGAGAGGTACAGGCTATAGATTCAGAATTTAACCAGGTTCTGCAGAATGATTCTTGCCGCCTTGAACAAATTCAGTGTCATACAGCCGCACCCGGTCACCCATTTAATAGATTCTGTTGGGGAAATAAGAAGAGCTTGGTTGATGCAATGGAAAAAGGGATCAACTTGCGAGAACAAATACTAAAGTTGTACAAGGATTATTACCATGGTGGACTGATGAAGCTAGTTGTCATTGGTGGAGAATCTCTTGATGTACTTGAGGATTGGGTTGTGGAATTGTATGGTAATGTCAAGAAAGGCCCCCAAGTAAAATTGGAGTTTAAGGCAGAAGGTCCTATCTGGAAAGTTGGCAAACTATACAGGCTAGAGGCTGTTAGAGATGTTAACATACTCAACCTTACATGGACATTTCCATGTCTTCATCAAGACTATTTGAAGAAACCAGAAGATTATTTAGCTCATCTACTTGGACATGAGGGCCGGGGAAGTTTGCATTTTTACCTCAAAACCAGAGGGTGGGCAACATCTTTGTCTGCTGGTGTTGGGGACGAGGGGATGCACCGATCTTCTGTGGCTTATGTCTTCCGCATGTCCATACACCTCACTGACTCTGGATTGGAAAAGATTTCCGAAATAATTTGCTATGTCTACCAATACATTAAGTTACTGCATCAAGTGTCTCCACAAGAATGGATATTTAGGGAACTTCAGGATATTGGGAATATGGATTTTAGATTTGCAGAGGAGCAGCCCCAAGATGATTATGCTGCAGAACTTGCAGAAAATTTGCTACTGTATCCAGCAGAGAACGTTATTTATGGGGACTATGTATACAAAATTTGGGACGCGGAATTGATAAAGTATGTTCTTGGTTTCTTCACACCAGAAAACATGAGGGTTGATGTGGTATCAAAGTCCTCAATTAAGTCGAAAGATTTCCATTGCGAGCCTTGGTTTGGGTCGCATTATACTGAGGAAGATATATCTCCATCTTTGATGGATATGTGGAAGAATCCCCCAGAAATTGATGACTCATTGTATCTCCCTTCAAAGAATGAGTTCATTCCTAGTGATTTTTCCATCCGCTCTGATAACTTGTGCCCTGATCCTGCAAATATATCTTATCCAAGCTGTGTAATTGATGAACCATTAATAAAGTTCTGGTACAAGCTCGATGATTCATTTAAACTTCCGCGGGCAAATACATACTTCCGCATCAATCTGAAGGGTGGATATGCTAATCTGAAAAGTTGTGTTTTGACTGAATTATATATTCTCCTTCTTAAGGATGAGCTGAATGAGATTGTGTACCAGGCCATTGTTGCCAAGCTGGAAACTTCTGTCTCTGTGTTCAGTGACAAACTGGAGCTGAAGGTCTATGGATTTAACGATAAACTTCCAGCTCTCTTGTCAAAAGTGTTGGCAACTGCCAAAAGTTTCATGCCAACAGATGATCGTTTTAAGGTCGTTAAAGAAGATATGAAGCGAAGGTTAAAGAACACCAATATGAAACCTCTGAGTCACTCATCATACTTAAGACTGCAAGTTCTGTGTCAAATTTTTTATGATGCAGATGAGAAGTTGCATGTTTTGGATGAATTGTCTGTTTCTGATTTGAAGTCGTTCATTCCTGCGCTTTGTTCCCAGCTATACATTGAGGGCCTTTGCCATGGAAATTTGTTAGAAGATGAAGCAATTACCCTGTCAAATATATTCAAATTGAATTTTTCTGTACCACCACTGCCTATTGAATTGAGGCATAAAGAGCATGTTATTTGTCTTCCTCCTGGTGCTAACCTGATTAGGGATTCCAATGTGAAGAATAAGTCAGAAACGAACTCTGTGATTGAGCTGTATTTTCAAGTTGAGCAAGAAGCAGGGATTGAGTCAATCCGATTGAAAACATTGATAGATCTTTTTGATGAAATTGTGGAGGAACCACTTTTCAATCAACTAAGGACGAAGGAGCAGCTTGGATATGTTGTTGAGTGTGGCCCCCGGGTAACATACCGTGTATATGGCTTTTGTTTCTGTGTCCAGTCGTCTGAGTACGACCCAATCTACTTACAAGGGAGAGTTGATAACTTTATAAACGGGCTGGAAGAAATGTTGGACGGGATTGATGATGATTCGTTTGAGAATTATAAAAGTGGACTACTTGCTAAGCTATTGGAGAAAGATCCATCCCTCACGTACGAAACCAACAGATTTTGGAATCAGATTATCGAGCAAAGGTATATGTTCGACCAGTCAAAAAGAGAAGCAGAAGAACTCGGCAGTATTCAGAAGAAGGACGTTATTGACTGGTACAAAACGTATTTGCAGCAATCATCTCCCAAGTGTCGTAGACTAGCGATTCGTGTTTGGGGTTGCAACACTGACCCGAGAGAGGCGGAACCACAACCGAAGTCTATCCAAGCCATCGAAGACCCTGCAACGTTCAAGAAGTCATCAAAGTTCTATCCTAGCCTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

1030

Amino Acids

118.2

Weight (kDa)

4.86

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 105 - 222 1.2e-31 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 261 - 441 4.1e-17 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 448 - 733 1.4e-86 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 738 - 923 8e-15 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 843 - 942 3.2e-25 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2702, 2757
Acc36I ACCTGC 1 cut(s) 521
AccB1I GGYRCC 1 cut(s) 551
AccB7I CCANNNNNTGG 1 cut(s) 1128
AccBSI CCGCTC 1 cut(s) 1698
AccI GTMKAC 3 cut(s) 1260, 1443, 2959
AccII CGCG 2 cut(s) 1460, 1807
AclI AACGTT 2 cut(s) 1422, 3050
AclWI GGATC 4 cut(s) 107, 779, 1712, 2786
AcsI RAATTY 7 cut(s) 638, 681, 1396, 1449, 2169, 2287, 2335
AcuI CTGAAG 5 cut(s) 195, 308, 1304, 1856, 2003
AfiI CCNNNNNNNGG 6 cut(s) 376, 1128, 1568, 1897, 2416, 3001
AflII CTTAAG 3 cut(s) 545, 1895, 2146
AjnI CCWGG 3 cut(s) 645, 1923, 2398
Alw26I GTCTC 3 cut(s) 303, 1296, 1960
AlwI GGATC 4 cut(s) 107, 779, 1712, 2786
AlwNI CAGNNNCTG 1 cut(s) 652
Ama87I CYCGRG 3 cut(s) 80, 2610, 2995
AoxI GGCC 6 cut(s) 134, 910, 1090, 1926, 2272, 2605
ApeKI GCWGC 9 cut(s) 9, 329, 332, 335, 357, 1360, 1379, 2581, 2935
ApoI RAATTY 7 cut(s) 638, 681, 1396, 1449, 2169, 2287, 2335
ArsI GACNNNNNNTTYG 4 cut(s) 673, 705, 2952, 2984
AseI ATTAAT 2 cut(s) 71, 1763
Asp700I GAANNNNTTC 5 cut(s) 565, 681, 1547, 1670, 2234
AspLEI GCGC 1 cut(s) 2248
AspS9I GGNCC 6 cut(s) 134, 911, 944, 1090, 2272, 2606
AsuC2I CCSGG 4 cut(s) 705, 1094, 2611, 2612
AsuHPI GGTGA 3 cut(s) 293, 576, 701
AvaI CYCGRG 3 cut(s) 80, 2610, 2995
AvaII GGWCC 1 cut(s) 944
BaeGI GKGCMC 1 cut(s) 1715
BanI GGYRCC 1 cut(s) 551
BbsI GAAGAC 5 cut(s) 234, 1022, 1186, 2384, 3045
BbvI GCAGC 8 cut(s) 341, 344, 347, 369, 1366, 1372, 2593, 2947
BccI CCATC 7 cut(s) 272, 601, 1603, 1609, 1700, 2803, 3041
BcgI CGANNNNNNTGC 2 cut(s) 3026, 3060
BciT130I CCWGG 3 cut(s) 647, 1925, 2400
BciVI GTATCC 1 cut(s) 1419
BclI TGATCA 1 cut(s) 142
BcnI CCSGG 4 cut(s) 705, 1094, 2611, 2612
BcoDI GTCTC 3 cut(s) 303, 1296, 1960
BfaI CTAG 7 cut(s) 401, 590, 836, 977, 1680, 2963, 3078
BfmI CTRYAG 3 cut(s) 627, 653, 1380
BfrI CTTAAG 3 cut(s) 545, 1895, 2146
BfuAI ACCTGC 1 cut(s) 521
BfuI GTATCC 1 cut(s) 1419
BglII AGATCT 1 cut(s) 2524
BlpI GCTNAGC 1 cut(s) 2775
Bme1390I CCNGG 7 cut(s) 647, 705, 1094, 1925, 2400, 2611, 2612
Bme18I GGWCC 1 cut(s) 944
BmeT110I CYCGRG 3 cut(s) 80, 2610, 2995
BmgT120I GGNCC 6 cut(s) 134, 911, 944, 1090, 2272, 2606
BmiI GGNNCC 5 cut(s) 553, 913, 2550, 2608, 3009
BmrFI CCNGG 7 cut(s) 647, 705, 1094, 1925, 2400, 2611, 2612
BmsI GCATC 5 cut(s) 745, 1158, 1291, 1836, 2170
BpiI GAAGAC 5 cut(s) 234, 1022, 1186, 2384, 3045
BpmI CTGGAG 1 cut(s) 1997
Bpu1102I GCTNAGC 1 cut(s) 2775
BpuEI CTTGAG 2 cut(s) 577, 890
BpuMI CCSGG 4 cut(s) 705, 1094, 2611, 2612
BsaAI YACGTR 1 cut(s) 2805
BsaJI CCNNGG 7 cut(s) 817, 1093, 1562, 1805, 2281, 2609, 2610
Bsc4I CCNNNNNNNGG 6 cut(s) 376, 1128, 1568, 1897, 2416, 3001
Bse1I ACTGG 4 cut(s) 1980, 2650, 2860, 2921
Bse3DI GCAATG 4 cut(s) 98, 345, 765, 1552
BseBI CCWGG 3 cut(s) 647, 1925, 2400
BseDI CCNNGG 7 cut(s) 817, 1093, 1562, 1805, 2281, 2609, 2610
BseGI GGATG 6 cut(s) 253, 1173, 1692, 1906, 2212, 2795
BseLI CCNNNNNNNGG 6 cut(s) 376, 1128, 1568, 1897, 2416, 3001
BseMI GCAATG 4 cut(s) 98, 345, 765, 1552
BseMII CTCAG 6 cut(s) 108, 329, 584, 1575, 2120, 2649
BseNI ACTGG 4 cut(s) 1980, 2650, 2860, 2921
BseRI GAGGAG 1 cut(s) 1370
BseSI GKGCMC 1 cut(s) 1715
BseXI GCAGC 8 cut(s) 341, 344, 347, 369, 1366, 1372, 2593, 2947
BseYI CCCAGC 1 cut(s) 2256
Bsh1236I CGCG 2 cut(s) 1460, 1807
BshFI GGCC 6 cut(s) 136, 912, 1092, 1928, 2274, 2607
BshNI GGYRCC 1 cut(s) 551
BsiHKCI CYCGRG 3 cut(s) 80, 2610, 2995
BsiSI CCGG 3 cut(s) 705, 1093, 2611
BsiWI CGTACG 1 cut(s) 2805
BslFI GGGAC 3 cut(s) 1172, 1448, 1469
BslI CCNNNNNNNGG 6 cut(s) 376, 1128, 1568, 1897, 2416, 3001
BsmAI GTCTC 3 cut(s) 303, 1296, 1960
BsmFI GGGAC 3 cut(s) 1172, 1448, 1469
BsnI GGCC 6 cut(s) 136, 912, 1092, 1928, 2274, 2607
BsoBI CYCGRG 3 cut(s) 80, 2610, 2995
Bsp1286I GDGCHC 1 cut(s) 1715
Bsp1407I TGTACA 1 cut(s) 801
Bsp143I GATC 8 cut(s) 112, 142, 771, 1175, 1717, 2065, 2524, 2791
Bsp1720I GCTNAGC 1 cut(s) 2775
Bsp19I CCATGG 2 cut(s) 817, 2281
BspANI GGCC 6 cut(s) 136, 912, 1092, 1928, 2274, 2607
BspCNI CTCAG 6 cut(s) 109, 328, 583, 1576, 2121, 2650
BspFNI CGCG 2 cut(s) 1460, 1807
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 5 cut(s) 553, 913, 2550, 2608, 3009
BspMAI CTGCAG 2 cut(s) 657, 1384
BspMI ACCTGC 1 cut(s) 521
BspPI GGATC 4 cut(s) 107, 779, 1712, 2786
BspQI GCTCTTC 2 cut(s) 419, 737
BspT107I GGYRCC 1 cut(s) 551
BspTI CTTAAG 3 cut(s) 545, 1895, 2146
BsrBI CCGCTC 1 cut(s) 1698
BsrDI GCAATG 4 cut(s) 98, 345, 765, 1552
BsrGI TGTACA 1 cut(s) 801
BsrI ACTGG 4 cut(s) 1980, 2650, 2860, 2921
BssECI CCNNGG 7 cut(s) 817, 1093, 1562, 1805, 2281, 2609, 2610
BssMI GATC 8 cut(s) 112, 142, 771, 1175, 1717, 2065, 2524, 2791
BssNAI GTATAC 1 cut(s) 1444
BssT1I CCWWGG 3 cut(s) 817, 1562, 2281
Bst1107I GTATAC 1 cut(s) 1444
Bst2UI CCWGG 3 cut(s) 647, 1925, 2400
Bst4CI ACNGT 4 cut(s) 167, 233, 1407, 2624
Bst6I CTCTTC 5 cut(s) 171, 180, 246, 419, 737
BstAFI CTTAAG 3 cut(s) 545, 1895, 2146
BstAPI GCANNNNNTGC 1 cut(s) 1388
BstAUI TGTACA 1 cut(s) 801
BstBAI YACGTR 1 cut(s) 2805
BstC8I GCNNGC 4 cut(s) 7, 390, 1559, 1809
BstDEI CTNAG 9 cut(s) 117, 315, 321, 570, 1584, 2129, 2567, 2658, 2775
BstDSI CCRYGG 3 cut(s) 817, 1805, 2281
BstEII GGTNACC 1 cut(s) 707
BstENI CCTNNNNNAGG 1 cut(s) 1895
BstF5I GGATG 6 cut(s) 253, 1173, 1692, 1906, 2212, 2795
BstFNI CGCG 2 cut(s) 1460, 1807
BstHHI GCGC 1 cut(s) 2248
BstKTI GATC 8 cut(s) 115, 145, 774, 1178, 1720, 2068, 2527, 2794
BstMAI GTCTC 3 cut(s) 303, 1296, 1960
BstMBI GATC 8 cut(s) 112, 142, 771, 1175, 1717, 2065, 2524, 2791
BstMWI GCNNNNNNNGC 4 cut(s) 335, 1388, 1934, 2377
BstNI CCWGG 3 cut(s) 647, 1925, 2400
BstNSI RCATGY 4 cut(s) 412, 1204, 2201, 2384
BstPI GGTNACC 1 cut(s) 707
BstSCI CCNGG 7 cut(s) 645, 703, 1092, 1923, 2398, 2609, 2610
BstSFI CTRYAG 3 cut(s) 627, 653, 1380
BstSLI GKGCMC 1 cut(s) 1715
BstUI CGCG 2 cut(s) 1460, 1807
BstV1I GCAGC 8 cut(s) 341, 344, 347, 369, 1366, 1372, 2593, 2947
BstV2I GAAGAC 5 cut(s) 234, 1022, 1186, 2384, 3045
BstX2I RGATCY 2 cut(s) 2524, 2791
BstXI CCANNNNNNTGG 1 cut(s) 1302
BstYI RGATCY 2 cut(s) 2524, 2791
BstZ17I GTATAC 1 cut(s) 1444
BsuI GTATCC 1 cut(s) 1419
BsuRI GGCC 6 cut(s) 136, 912, 1092, 1928, 2274, 2607
BtgI CCRYGG 3 cut(s) 817, 1805, 2281
BtgZI GCGATG 1 cut(s) 620
BtsCI GGATG 6 cut(s) 253, 1173, 1692, 1906, 2212, 2795
BtsI GCAGTG 1 cut(s) 2351
BtsIMutI CAGTG 7 cut(s) 172, 238, 692, 1215, 1972, 2351, 2988
BveI ACCTGC 1 cut(s) 521
Cac8I GCNNGC 4 cut(s) 7, 390, 1559, 1809
CaiI CAGNNNCTG 1 cut(s) 652
CciI TCATGA 1 cut(s) 109
CfoI GCGC 1 cut(s) 2248
Cfr13I GGNCC 6 cut(s) 134, 911, 944, 1090, 2272, 2606
Cfr42I CCGCGG 1 cut(s) 1808
Cfr9I CCCGGG 1 cut(s) 2610
CseI GACGC 1 cut(s) 1466
CsiI ACCWGGT 1 cut(s) 645
CspCI CAANNNNNGTGG 2 cut(s) 327, 362
DdeI CTNAG 9 cut(s) 117, 315, 321, 570, 1584, 2129, 2567, 2658, 2775
DpnI GATC 8 cut(s) 114, 144, 773, 1177, 1719, 2067, 2526, 2793
DpnII GATC 8 cut(s) 112, 142, 771, 1175, 1717, 2065, 2524, 2791
DraI TTTAAA 1 cut(s) 1798
Eam1104I CTCTTC 5 cut(s) 171, 180, 246, 419, 737
EarI CTCTTC 5 cut(s) 171, 180, 246, 419, 737
EciI GGCGGA 2 cut(s) 288, 3020
Eco130I CCWWGG 3 cut(s) 817, 1562, 2281
Eco32I GATATC 1 cut(s) 31
Eco47I GGWCC 1 cut(s) 944
Eco57I CTGAAG 5 cut(s) 195, 308, 1304, 1856, 2003
Eco88I CYCGRG 3 cut(s) 80, 2610, 2995
Eco91I GGTNACC 1 cut(s) 707
EcoNI CCTNNNNNAGG 1 cut(s) 1895
EcoO109I RGGNCCY 3 cut(s) 911, 944, 2272
EcoO65I GGTNACC 1 cut(s) 707
EcoRII CCWGG 3 cut(s) 645, 1923, 2398
EcoRV GATATC 1 cut(s) 31
EcoT14I CCWWGG 3 cut(s) 817, 1562, 2281
EcoT22I ATGCAT 1 cut(s) 493
ErhI CCWWGG 3 cut(s) 817, 1562, 2281
FalI AAGNNNNNCTT 4 cut(s) 539, 571, 731, 763
FaqI GGGAC 3 cut(s) 1172, 1448, 1469
FauI CCCGC 1 cut(s) 1800
FbaI TGATCA 1 cut(s) 142
FblI GTMKAC 3 cut(s) 1260, 1443, 2959
FokI GGATG 6 cut(s) 260, 1180, 1679, 1913, 2219, 2782
FspBI CTAG 7 cut(s) 401, 590, 836, 977, 1680, 2963, 3078
GlaI GCGC 1 cut(s) 2247
GsaI CCCAGC 1 cut(s) 2260
GsuI CTGGAG 1 cut(s) 1997
HaeIII GGCC 6 cut(s) 136, 912, 1092, 1928, 2274, 2607
HapII CCGG 3 cut(s) 705, 1093, 2611
HgaI GACGC 1 cut(s) 1466
HhaI GCGC 1 cut(s) 2248
Hin6I GCGC 1 cut(s) 2246
HinP1I GCGC 1 cut(s) 2246
HincII GTYRAC 1 cut(s) 997
HindII GTYRAC 1 cut(s) 997
HpaI GTTAAC 1 cut(s) 997
HpaII CCGG 3 cut(s) 705, 1093, 2611
HphI GGTGA 3 cut(s) 293, 576, 701
Hpy166II GTNNAC 8 cut(s) 284, 824, 997, 1261, 1444, 1921, 2765, 2960
Hpy8I GTNNAC 8 cut(s) 284, 824, 997, 1261, 1444, 1921, 2765, 2960
Hpy99I CGWCG 2 cut(s) 161, 209
HpyCH4III ACNGT 4 cut(s) 167, 233, 1407, 2624
HpyCH4IV ACGT 5 cut(s) 1422, 2804, 2907, 2927, 3050
HpyF10VI GCNNNNNNNGC 4 cut(s) 335, 1388, 1934, 2377
HpyF3I CTNAG 9 cut(s) 117, 315, 321, 570, 1584, 2129, 2567, 2658, 2775
HpySE526I ACGT 5 cut(s) 1422, 2804, 2907, 2927, 3050
HspAI GCGC 1 cut(s) 2246
Ksp22I TGATCA 1 cut(s) 142
KspAI GTTAAC 1 cut(s) 997
KspI CCGCGG 1 cut(s) 1808
Kzo9I GATC 8 cut(s) 112, 142, 771, 1175, 1717, 2065, 2524, 2791
LguI GCTCTTC 2 cut(s) 419, 737
LmnI GCTCC 4 cut(s) 610, 1357, 1978, 2578
Lsp1109I GCAGC 8 cut(s) 341, 344, 347, 369, 1366, 1372, 2593, 2947
LweI GCATC 5 cut(s) 745, 1158, 1291, 1836, 2170
MabI ACCWGGT 1 cut(s) 645
MaeI CTAG 7 cut(s) 401, 590, 836, 977, 1680, 2963, 3078
MaeII ACGT 5 cut(s) 1422, 2804, 2907, 2927, 3050
MaeIII GTNAC 6 cut(s) 458, 707, 1275, 1967, 2132, 2614
MalI GATC 8 cut(s) 114, 144, 773, 1177, 1719, 2067, 2526, 2793
MbiI CCGCTC 1 cut(s) 1698
MboI GATC 8 cut(s) 112, 142, 771, 1175, 1717, 2065, 2524, 2791
MflI RGATCY 2 cut(s) 2524, 2791
MhlI GDGCHC 1 cut(s) 1715
MlyI GAGTC 5 cut(s) 305, 1214, 1637, 2140, 2507
MmeI TCCRAC 2 cut(s) 390, 2703
Mph1103I ATGCAT 1 cut(s) 493
MroXI GAANNNNTTC 5 cut(s) 565, 681, 1547, 1670, 2234
MslI CAYNNNNRTG 3 cut(s) 1024, 1300, 2117
MspA1I CMGCKG 1 cut(s) 1807
MspCI CTTAAG 3 cut(s) 545, 1895, 2146
MspI CCGG 3 cut(s) 705, 1093, 2611
MspR9I CCNGG 7 cut(s) 647, 705, 1094, 1925, 2400, 2611, 2612
MvaI CCWGG 3 cut(s) 647, 1925, 2400
MvnI CGCG 2 cut(s) 1460, 1807
MwoI GCNNNNNNNGC 4 cut(s) 335, 1388, 1934, 2377
NciI CCSGG 4 cut(s) 705, 1094, 2611, 2612
NcoI CCATGG 2 cut(s) 817, 2281
NdeII GATC 8 cut(s) 112, 142, 771, 1175, 1717, 2065, 2524, 2791
NlaIV GGNNCC 5 cut(s) 553, 913, 2550, 2608, 3009
NmeAIII GCCGAG 2 cut(s) 74, 2865
NmuCI GTSAC 3 cut(s) 707, 1967, 2132
NsiI ATGCAT 1 cut(s) 493
NspI RCATGY 4 cut(s) 412, 1204, 2201, 2384
PaeR7I CTCGAG 1 cut(s) 80
PagI TCATGA 1 cut(s) 109
PciSI GCTCTTC 2 cut(s) 419, 737
PdmI GAANNNNTTC 5 cut(s) 565, 681, 1547, 1670, 2234
Pfl23II CGTACG 1 cut(s) 2805
PflMI CCANNNNNTGG 1 cut(s) 1128
PleI GAGTC 5 cut(s) 305, 1214, 1637, 2139, 2506
PpsI GAGTC 5 cut(s) 305, 1214, 1637, 2139, 2506
Ppu21I YACGTR 1 cut(s) 2805
PpuMI RGGWCCY 1 cut(s) 944
PshBI ATTAAT 2 cut(s) 71, 1763
PsiI TTATAA 2 cut(s) 2702, 2757
Psp1406I AACGTT 2 cut(s) 1422, 3050
Psp5II RGGWCCY 1 cut(s) 944
Psp6I CCWGG 3 cut(s) 645, 1923, 2398
PspEI GGTNACC 1 cut(s) 707
PspFI CCCAGC 1 cut(s) 2256
PspGI CCWGG 3 cut(s) 645, 1923, 2398
PspLI CGTACG 1 cut(s) 2805
PspN4I GGNNCC 5 cut(s) 553, 913, 2550, 2608, 3009
PspPI GGNCC 6 cut(s) 134, 911, 944, 1090, 2272, 2606
PspPPI RGGWCCY 1 cut(s) 944
PstI CTGCAG 2 cut(s) 657, 1384
PstNI CAGNNNCTG 1 cut(s) 652
PsuI RGATCY 2 cut(s) 2524, 2791
RseI CAYNNNNRTG 3 cut(s) 1024, 1300, 2117
SacII CCGCGG 1 cut(s) 1808
SapI GCTCTTC 2 cut(s) 419, 737
Sau3AI GATC 8 cut(s) 112, 142, 771, 1175, 1717, 2065, 2524, 2791
Sau96I GGNCC 6 cut(s) 134, 911, 944, 1090, 2272, 2606
SchI GAGTC 5 cut(s) 305, 1214, 1637, 2140, 2507
ScrFI CCNGG 7 cut(s) 647, 705, 1094, 1925, 2400, 2611, 2612
SduI GDGCHC 1 cut(s) 1715
SexAI ACCWGGT 1 cut(s) 645
SfaNI GCATC 5 cut(s) 745, 1158, 1291, 1836, 2170
SfcI CTRYAG 3 cut(s) 627, 653, 1380
Sfr274I CTCGAG 1 cut(s) 80
Sfr303I CCGCGG 1 cut(s) 1808
SgrBI CCGCGG 1 cut(s) 1808
SinI GGWCC 1 cut(s) 944
SlaI CTCGAG 1 cut(s) 80
SmaI CCCGGG 1 cut(s) 2612
SmiMI CAYNNNNRTG 3 cut(s) 1024, 1300, 2117
SmlI CTYRAG 6 cut(s) 80, 545, 556, 869, 1895, 2146
SmoI CTYRAG 6 cut(s) 80, 545, 556, 869, 1895, 2146
SspMI CTAG 7 cut(s) 401, 590, 836, 977, 1680, 2963, 3078
StyD4I CCNGG 7 cut(s) 645, 703, 1092, 1923, 2398, 2609, 2610
StyI CCWWGG 3 cut(s) 817, 1562, 2281
TaaI ACNGT 4 cut(s) 167, 233, 1407, 2624
TaiI ACGT 5 cut(s) 1425, 2807, 2910, 2930, 3053
TaqI TCGA 7 cut(s) 81, 159, 1541, 1785, 2838, 2856, 3036
TatI WGTACW 3 cut(s) 425, 801, 865
TauI GCSGC 3 cut(s) 139, 672, 701
TscAI CASTG 7 cut(s) 172, 238, 692, 1222, 1972, 2358, 2995
TseFI GTSAC 3 cut(s) 707, 1967, 2132
TseI GCWGC 9 cut(s) 9, 329, 332, 335, 357, 1360, 1379, 2581, 2935
Tsp45I GTSAC 3 cut(s) 707, 1967, 2132
TspGWI ACGGA 2 cut(s) 145, 228
TspMI CCCGGG 1 cut(s) 2610
TspRI CASTG 7 cut(s) 172, 238, 692, 1222, 1972, 2358, 2995
Van91I CCANNNNNTGG 1 cut(s) 1128
Vha464I CTTAAG 3 cut(s) 545, 1895, 2146
VpaK11BI GGWCC 1 cut(s) 944
VspI ATTAAT 2 cut(s) 71, 1763
XagI CCTNNNNNAGG 1 cut(s) 1895
XapI RAATTY 7 cut(s) 638, 681, 1396, 1449, 2169, 2287, 2335
XbaI TCTAGA 1 cut(s) 400
XceI RCATGY 4 cut(s) 412, 1204, 2201, 2384
XhoI CTCGAG 1 cut(s) 80
XmaI CCCGGG 1 cut(s) 2610
XmiI GTMKAC 3 cut(s) 1260, 1443, 2959
XmnI GAANNNNTTC 5 cut(s) 565, 681, 1547, 1670, 2234
XspI CTAG 7 cut(s) 401, 590, 836, 977, 1680, 2963, 3078
Zsp2I ATGCAT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.