Rw0G011760

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00509
OG0000891
Physical Location & Seq
Reverse (-)
598 .. 8749
8152 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G011760.1

Sequence Viewer

Length: 3000 bp
ATGGCTCGCCGGACTTTCTCATCGGACGACGTCGTTTTGAAGTCTCCGAATGATAGACGACTGTACCGATTAATAGAGCTCGAGAATGGCCTCACTGCATTGCTAGTCCACGATCCTGAGATTGGAGATTCCCAACTCTCCAATGGCTCTGAGGAAGCTGAGATGGAAGAAGAAGAAGAAGGAATGGAGGAGGATGAGGAAGACGACGATGAAGAAGACGAAGATAGTGAAGGAGAGGATGAAGAGATGGATGAAGATGATGAGGGAGAAGATGAAGATGGACTGAAGAAGAAAAAGGGAGGGGATTCTCAGACTAAGAAGGCAGCAGCGGCAATGTGTGTAGGAATAGGCAGCTTCTCTGACCCTCCTGAGGCACCGGGGCTTGCACACTTTCTAGAACACATGCTCTTTATGGGGAGTACAGAATTTCCAGATGAAAATGAGTATGACAGTTACTTGTCCAAGCATGGAGGGTGTTCAAATGCATACACGGAGGTAGAGCATACTTGCTACCATTTTGAAGTGAAACGAGAGTTTCTCAAGGGTGCCTTGACCAGATTTTCTCAGTTCTTTGTTTCACCCCTTGTAAAAATTGAAGCCATGGAGCGGGAGGTACAGGCTGTAGATTCAGAGTTTAACCAGGTTCAGCAGAACGATGCTTGCCGCCTTGAACAACTTCAATGCCATACAGCCTCACCTGGTCACCCATTTAATAGATTCGCATGGGGTAAAAATTATATCTTGGGCAATGCCATGGAAAAAGGGATCAACTTGCGGGAACAAATATTGAAATTGTACAGAGATTATTACCATGGTGGATTGATGAAGCTAGTTGTCATAGGAGGAGAATCTCTTGATGTACTTGAGAATTGGGTTTTGGAATTGTTTGGTGACGTCAAAAAAGGTCCCCAAGTAAATCTGGAATTCAAGGCGGAAGGTCCCATTTGGAAAGCTGGAAAAGTTTACAGGCTAGAGGCTGTTAAAGATGTTCATATACTCCACTTAACATGGACATTGCCATGCCTTCGTCAAGACTATTTGAAGAAATCAGAAGATTATTTATCTCATCTCCTTGGGCATGAGGGCAGGGGAAGTTTGCATTCCCATTTCAAAGTTAAGGGGTGGGCAACATCTCTGGCTGCTGGTGTTGGGGATGACGGGATGCATCGTTCTTCTGTGTCTTATGTCTTTCGCATGGACATTCATCTCACTGACTCTGGATTGGATAAGATTTTTGATATAATTGGCTTGGTCTACCAATACATAAAGTTATTGCATCAAATGTCCCCACAAGAATGGATATTTAAGGAACTGCAGGATACTGGGAACATGGAATTTAGATTTGCAGAGGAGCAGCCTCAGGATGATTATGCCTCAGAACTTCAACATGTTATTTATGGGTCCTACGCATACAAGATTTGGGATGAGGAATCAATCAAATATGTTCTTGATTTCCTTAGACCAGAAAACATGAGGATTGATGTTGTATCAAAGCCCTCATTTAAGTCAGAAGATTTCCAGTGCGAACCGTGGTTTGGGTCACATTATACTGAGGAAGATATATCTCCATCTTTGATAGATTTGTGGAAGGACCCTCCAGAAATTGATGTTTCATTGCATCTCCCAGAAAAGAATGAGTTCATTCCTACTGATTTTTCCATTCGTTCTGAAGATCTTGATACTACAGATACAGCTTTTCCGAGATGTATACTGGATGAGCCATTGGTGAAGTTCTGGTACAAACTTGATAATACTTTTAAACTTCCGCGGGCAAATACTTATTTCCGCATCAATCCCAAGGGTGGATATGATAATGTGAAGAGTTGTGTCTTGACTGAACTATATGTATCCCTCCTCAAAGATGAGCTGAATGAGATTGTTTATCAGGCTAGTGTTGCCAAGCTGGAAACTTCGGTATCTGTATCGACTGACAAGCTGGAGCTAAAGGTCTACGGTTTCAATGACAAGCTTCCAGCTCTGTTGTCAAAGATTCTGAAAACAACCAAAAGTTTCATGCCAACTTCTGATCGTTTTATGGTTATCAAAGAAGATATGGAGCGAAAGTTAAAGAACACCAATATGAAGCCTCTGAGTCACTCTACATACTTGAGAGTGCAAGTTTTGTTCCAGAGATTCTATGATGTTGATGAGAAGTTGCATGTTTTAAGTGGACTGTCTATTTCTGATATGAAGTCATTTATTCCCCAACTTTGGTCCCAGGTATACATTGAAGGCCTCTGTCATGGAAATTTGTCAGAAAAAGAAGCAATCAGCCTTCAGATATTTAAAACAAATTTTTGTGTACAACCACTTCCAATTGAACTGAGGCGCAGTGAACAGTGTATTTGTCTTCCTCCTTCTGCTAACCTCATTAGAGATGTCAGTGTGAAGAATCAGTCTGAAAAAAATTCAGTGATTGAGGATGAGTCCCCCAGATTGAGAGCCCTGATAGATCTTTTCAATGAAATTGTGGAGGAACCACTTTTCAATCAGCTAAGGACGAAGGAGCAGCTTGGTTATACTGTTCATTGTGGCCCACGGGTTACTTGCAATATATTTGGATTTTATTTTTGCGTTCAGTCAGCTGAGTACAACCCAATCTACTTGCAAGGGAGAGTTGAGAACTTTATAATACAAGGACTGGATGATGACTCTTTTGAGGATTACAGAGCTGGGCTAATTGCAAAGCTGTTGGAGAAAGATCCATCCCTCCATTATGAAACCAATCGATATTGGTTACAGATTACCGATAAAATGTATATGTTTGACTATACAAAACAGGAGGCAGAACGACTCAAGAGCATTCAGAAGGAGGATGTCATCAACTGGTACAAAACATATTTGCAACAATCATCTCCCAAGTGTCGGAGACTTGCAATTCGTGTTTGGGGATGCAACACAGACTTGAAAGAGGCTGAAGCACGGCCGGAGTCTGTAAAAGTCATTGAAGACCTTGCAGCCTTTAAGATGTCATCTGAGTTCTATCCAAGCATTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

999

Amino Acids

115.29

Weight (kDa)

4.85

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 103 - 226 1e-32 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 259 - 439 8.1e-19 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 446 - 720 4.1e-83 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 726 - 890 2.3e-08 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 819 - 912 5.6e-20 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2630
AatII GACGTC 2 cut(s) 33, 897
AccB1I GGYRCC 2 cut(s) 373, 545
AccBSI CCGCTC 1 cut(s) 607
AccI GTMKAC 4 cut(s) 1254, 1708, 1950, 2223
AccII CGCG 1 cut(s) 1768
AciI CCGC 8 cut(s) 329, 607, 664, 775, 932, 1766, 1768, 1786
AclWI GGATC 3 cut(s) 107, 773, 2696
AcoI YGGCCR 1 cut(s) 2924
AcsI RAATTY 6 cut(s) 425, 923, 1334, 2248, 2293, 2407
AcuI CTGAAG 4 cut(s) 305, 1689, 2261, 2937
AcyI GRCGYC 2 cut(s) 30, 894
AfaI GTAC 9 cut(s) 65, 421, 615, 797, 861, 1739, 2304, 2591, 2831
AfiI CCNNNNNNNGG 7 cut(s) 122, 370, 606, 1535, 1802, 2211, 2865
AflIII ACRYGT 1 cut(s) 1387
AjnI CCWGG 3 cut(s) 639, 697, 2217
AjuI GAANNNNNNNTTGG 4 cut(s) 860, 892, 1518, 1550
AloI GAACNNNNNNTCC 2 cut(s) 1153, 1185
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 2 cut(s) 48, 2863
AlwI GGATC 3 cut(s) 107, 773, 2696
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 4 cut(s) 88, 2233, 2533, 2924
ApeKI GCWGC 7 cut(s) 323, 326, 351, 1139, 1354, 2509, 2957
ApoI RAATTY 6 cut(s) 425, 923, 1334, 2248, 2293, 2407
ArsI GACNNNNNNTTYG 2 cut(s) 2862, 2894
AseI ATTAAT 1 cut(s) 71
Asp700I GAANNNNTTC 2 cut(s) 675, 1637
AspLEI GCGC 1 cut(s) 2331
AspS9I GGNCC 6 cut(s) 905, 938, 1401, 1591, 2214, 2534
AsuC2I CCSGG 1 cut(s) 378
AsuHPI GGTGA 5 cut(s) 570, 687, 695, 902, 1738
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 5 cut(s) 905, 938, 1401, 1591, 2214
AxyI CCTNAGG 2 cut(s) 369, 1359
BaeI ACNNNNGTAYC 4 cut(s) 47, 80, 1899, 1932
BanI GGYRCC 2 cut(s) 373, 545
BanII GRGCYC 2 cut(s) 81, 2446
BarI GAAGNNNNNNTAC 4 cut(s) 1721, 1753, 2295, 2327
BbsI GAAGAC 4 cut(s) 207, 222, 2342, 2955
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 7 cut(s) 335, 338, 363, 1126, 1366, 2521, 2969
BccI CCATC 5 cut(s) 157, 241, 272, 1576, 2713
BceAI ACGGC 1 cut(s) 2939
BciT130I CCWGG 3 cut(s) 641, 699, 2219
BciVI GTATCC 2 cut(s) 1312, 1858
BcnI CCSGG 1 cut(s) 378
BcoDI GTCTC 2 cut(s) 48, 2863
BfaI CTAG 5 cut(s) 104, 395, 830, 971, 1890
BfmI CTRYAG 3 cut(s) 621, 1313, 1683
BfuI GTATCC 2 cut(s) 1312, 1858
BglII AGATCT 2 cut(s) 1672, 2452
BisI GCNGC 9 cut(s) 324, 327, 330, 352, 664, 1140, 1355, 2510, 2958
BlsI GCNGC 9 cut(s) 325, 328, 331, 353, 665, 1141, 1356, 2511, 2959
Bme1390I CCNGG 4 cut(s) 378, 641, 699, 2219
Bme18I GGWCC 5 cut(s) 905, 938, 1401, 1591, 2214
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 6 cut(s) 905, 938, 1401, 1591, 2214, 2534
BmiI GGNNCC 8 cut(s) 375, 547, 907, 940, 1402, 1593, 2216, 2478
BmrFI CCNGG 4 cut(s) 378, 641, 699, 2219
BmrI ACTGGG 1 cut(s) 1332
BmsI GCATC 7 cut(s) 646, 1152, 1174, 1285, 1627, 1797, 2882
BmuI ACTGGG 1 cut(s) 1332
BpiI GAAGAC 4 cut(s) 207, 222, 2342, 2955
BplI GAGNNNNNCTC 2 cut(s) 522, 554
BpmI CTGGAG 2 cut(s) 1581, 1958
Bpu10I CCTNAGC 1 cut(s) 2495
BpuEI CTTGAG 4 cut(s) 524, 884, 2128, 2780
BpuMI CCSGG 1 cut(s) 378
Bsa29I ATCGAT 1 cut(s) 2728
BsaHI GRCGYC 2 cut(s) 30, 894
BsaXI ACNNNNNCTCC 2 cut(s) 2048, 2078
Bsc4I CCNNNNNNNGG 7 cut(s) 122, 370, 606, 1535, 1802, 2211, 2865
Bse1I ACTGG 5 cut(s) 1327, 1519, 1716, 2646, 2831
Bse21I CCTNAGG 2 cut(s) 369, 1359
Bse3DI GCAATG 5 cut(s) 98, 339, 754, 1013, 1613
BseBI CCWGG 3 cut(s) 641, 699, 2219
BseCI ATCGAT 1 cut(s) 2728
BseLI CCNNNNNNNGG 7 cut(s) 122, 370, 606, 1535, 1802, 2211, 2865
BseMI GCAATG 5 cut(s) 98, 339, 754, 1013, 1613
BseNI ACTGG 5 cut(s) 1327, 1519, 1716, 2646, 2831
BseRI GAGGAG 4 cut(s) 203, 858, 1364, 1844
BseX3I CGGCCG 1 cut(s) 2924
BseXI GCAGC 7 cut(s) 335, 338, 363, 1126, 1366, 2521, 2969
BseYI CCCAGC 1 cut(s) 2672
Bsh1236I CGCG 1 cut(s) 1768
Bsh1285I CGRYCG 1 cut(s) 2927
BshFI GGCC 4 cut(s) 90, 2235, 2535, 2926
BshNI GGYRCC 2 cut(s) 373, 545
BshVI ATCGAT 1 cut(s) 2728
BsiEI CGRYCG 1 cut(s) 2927
BsiHKAI GWGCWC 1 cut(s) 81
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 3 cut(s) 10, 377, 2927
BslFI GGGAC 5 cut(s) 891, 924, 1270, 2200, 2413
BslI CCNNNNNNNGG 7 cut(s) 122, 370, 606, 1535, 1802, 2211, 2865
BsmAI GTCTC 2 cut(s) 48, 2863
BsmFI GGGAC 5 cut(s) 891, 924, 1270, 2200, 2413
BsmI GAATGC 2 cut(s) 1099, 2802
BsnI GGCC 4 cut(s) 90, 2235, 2535, 2926
BsoBI CYCGRG 1 cut(s) 80
Bsp1286I GDGCHC 2 cut(s) 81, 2446
Bsp1407I TGTACA 2 cut(s) 795, 2302
Bsp143I GATC 6 cut(s) 112, 765, 1672, 2026, 2452, 2701
Bsp19I CCATGG 3 cut(s) 600, 753, 811
BspACI CCGC 8 cut(s) 329, 607, 664, 775, 932, 1766, 1768, 1786
BspANI GGCC 4 cut(s) 90, 2235, 2535, 2926
BspDI ATCGAT 1 cut(s) 2728
BspFNI CGCG 1 cut(s) 1768
BspLI GGNNCC 8 cut(s) 375, 547, 907, 940, 1402, 1593, 2216, 2478
BspMAI CTGCAG 1 cut(s) 1317
BspPI GGATC 3 cut(s) 107, 773, 2696
BspT107I GGYRCC 2 cut(s) 373, 545
BsrBI CCGCTC 1 cut(s) 607
BsrDI GCAATG 5 cut(s) 98, 339, 754, 1013, 1613
BsrGI TGTACA 2 cut(s) 795, 2302
BsrI ACTGG 5 cut(s) 1327, 1519, 1716, 2646, 2831
BssMI GATC 6 cut(s) 112, 765, 1672, 2026, 2452, 2701
BssNAI GTATAC 2 cut(s) 1709, 2224
BssNI GRCGYC 2 cut(s) 30, 894
BssT1I CCWWGG 5 cut(s) 600, 753, 811, 1072, 1797
Bst1107I GTATAC 2 cut(s) 1709, 2224
Bst2UI CCWGG 3 cut(s) 641, 699, 2219
Bst4CI ACNGT 7 cut(s) 63, 452, 1530, 1955, 2175, 2340, 2524
Bst6I CTCTTC 2 cut(s) 237, 1814
BstACI GRCGYC 2 cut(s) 30, 894
BstAUI TGTACA 2 cut(s) 795, 2302
BstC8I GCNNGC 4 cut(s) 7, 384, 661, 1770
BstDSI CCRYGG 6 cut(s) 600, 753, 811, 1529, 1766, 2537
BstEII GGTNACC 1 cut(s) 701
BstFNI CGCG 1 cut(s) 1768
BstHHI GCGC 1 cut(s) 2331
BstKTI GATC 6 cut(s) 115, 768, 1675, 2029, 2455, 2704
BstMAI GTCTC 2 cut(s) 48, 2863
BstMBI GATC 6 cut(s) 112, 765, 1672, 2026, 2452, 2701
BstMCI CGRYCG 1 cut(s) 2927
BstMWI GCNNNNNNNGC 2 cut(s) 329, 1895
BstNI CCWGG 3 cut(s) 641, 699, 2219
BstNSI RCATGY 3 cut(s) 406, 1391, 2162
BstPI GGTNACC 1 cut(s) 701
BstSCI CCNGG 4 cut(s) 376, 639, 697, 2217
BstSFI CTRYAG 3 cut(s) 621, 1313, 1683
BstUI CGCG 1 cut(s) 1768
BstV1I GCAGC 7 cut(s) 335, 338, 363, 1126, 1366, 2521, 2969
BstV2I GAAGAC 4 cut(s) 207, 222, 2342, 2955
BstX2I RGATCY 3 cut(s) 1672, 2452, 2701
BstXI CCANNNNNNTGG 1 cut(s) 1296
BstYI RGATCY 3 cut(s) 1672, 2452, 2701
BstZ17I GTATAC 2 cut(s) 1709, 2224
BstZI CGGCCG 1 cut(s) 2924
Bsu15I ATCGAT 1 cut(s) 2728
Bsu36I CCTNAGG 2 cut(s) 369, 1359
BsuI GTATCC 2 cut(s) 1312, 1858
BsuRI GGCC 4 cut(s) 90, 2235, 2535, 2926
BsuTUI ATCGAT 1 cut(s) 2728
BtgI CCRYGG 6 cut(s) 600, 753, 811, 1529, 1766, 2537
BtsI GCAGTG 2 cut(s) 93, 2338
BtsIMutI CAGTG 7 cut(s) 93, 1209, 1526, 2338, 2345, 2389, 2418
Cac8I GCNNGC 4 cut(s) 7, 384, 661, 1770
CfoI GCGC 1 cut(s) 2331
Cfr13I GGNCC 6 cut(s) 905, 938, 1401, 1591, 2214, 2534
Cfr42I CCGCGG 1 cut(s) 1769
ClaI ATCGAT 1 cut(s) 2728
CsiI ACCWGGT 2 cut(s) 639, 697
Csp6I GTAC 9 cut(s) 64, 420, 614, 796, 860, 1738, 2303, 2590, 2830
CviQI GTAC 9 cut(s) 64, 420, 614, 796, 860, 1738, 2303, 2590, 2830
DpnI GATC 6 cut(s) 114, 767, 1674, 2028, 2454, 2703
DpnII GATC 6 cut(s) 112, 765, 1672, 2026, 2452, 2701
DraI TTTAAA 2 cut(s) 1759, 2287
EaeI YGGCCR 1 cut(s) 2924
EagI CGGCCG 1 cut(s) 2924
Eam1104I CTCTTC 2 cut(s) 237, 1814
EarI CTCTTC 2 cut(s) 237, 1814
EciI GGCGGA 1 cut(s) 947
Ecl136II GAGCTC 1 cut(s) 79
EclXI CGGCCG 1 cut(s) 2924
Eco130I CCWWGG 5 cut(s) 600, 753, 811, 1072, 1797
Eco147I AGGCCT 1 cut(s) 2235
Eco24I GRGCYC 2 cut(s) 81, 2446
Eco47I GGWCC 5 cut(s) 905, 938, 1401, 1591, 2214
Eco52I CGGCCG 1 cut(s) 2924
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 4 cut(s) 305, 1689, 2261, 2937
Eco81I CCTNAGG 2 cut(s) 369, 1359
Eco88I CYCGRG 1 cut(s) 80
Eco91I GGTNACC 1 cut(s) 701
EcoICRI GAGCTC 1 cut(s) 79
EcoO109I RGGNCCY 4 cut(s) 905, 938, 1401, 1591
EcoO65I GGTNACC 1 cut(s) 701
EcoRI GAATTC 1 cut(s) 923
EcoRII CCWGG 3 cut(s) 639, 697, 2217
EcoT14I CCWWGG 5 cut(s) 600, 753, 811, 1072, 1797
EcoT22I ATGCAT 2 cut(s) 487, 1167
EcoT38I GRGCYC 2 cut(s) 81, 2446
ErhI CCWWGG 5 cut(s) 600, 753, 811, 1072, 1797
FalI AAGNNNNNCTT 2 cut(s) 533, 565
FaqI GGGAC 5 cut(s) 891, 924, 1270, 2200, 2413
FauI CCCGC 3 cut(s) 600, 768, 1761
FblI GTMKAC 4 cut(s) 1254, 1708, 1950, 2223
Fnu4HI GCNGC 9 cut(s) 324, 327, 330, 352, 664, 1140, 1355, 2510, 2958
FriOI GRGCYC 2 cut(s) 81, 2446
Fsp4HI GCNGC 9 cut(s) 324, 327, 330, 352, 664, 1140, 1355, 2510, 2958
FspBI CTAG 5 cut(s) 104, 395, 830, 971, 1890
GlaI GCGC 1 cut(s) 2330
GluI GCNGC 9 cut(s) 324, 327, 330, 352, 664, 1140, 1355, 2510, 2958
GsaI CCCAGC 1 cut(s) 2676
GsuI CTGGAG 2 cut(s) 1581, 1958
HaeIII GGCC 4 cut(s) 90, 2235, 2535, 2926
HapII CCGG 3 cut(s) 10, 377, 2927
HhaI GCGC 1 cut(s) 2331
Hin1I GRCGYC 2 cut(s) 30, 894
Hin6I GCGC 1 cut(s) 2329
HinP1I GCGC 1 cut(s) 2329
HindIII AAGCTT 1 cut(s) 1967
HpaII CCGG 3 cut(s) 10, 377, 2927
HphI GGTGA 5 cut(s) 570, 687, 695, 902, 1738
Hpy166II GTNNAC 9 cut(s) 109, 964, 1255, 1709, 1951, 2171, 2224, 2303, 2336
Hpy8I GTNNAC 9 cut(s) 109, 964, 1255, 1709, 1951, 2171, 2224, 2303, 2336
Hpy99I CGWCG 3 cut(s) 32, 35, 209
HpyCH4III ACNGT 7 cut(s) 63, 452, 1530, 1955, 2175, 2340, 2524
HpyCH4IV ACGT 2 cut(s) 30, 894
HpyF10VI GCNNNNNNNGC 2 cut(s) 329, 1895
HpySE526I ACGT 2 cut(s) 30, 894
Hsp92I GRCGYC 2 cut(s) 30, 894
HspAI GCGC 1 cut(s) 2329
KspI CCGCGG 1 cut(s) 1769
Kzo9I GATC 6 cut(s) 112, 765, 1672, 2026, 2452, 2701
LmnI GCTCC 5 cut(s) 604, 1351, 1939, 2056, 2506
Lsp1109I GCAGC 7 cut(s) 335, 338, 363, 1126, 1366, 2521, 2969
LweI GCATC 7 cut(s) 646, 1152, 1174, 1285, 1627, 1797, 2882
MabI ACCWGGT 2 cut(s) 639, 697
MaeI CTAG 5 cut(s) 104, 395, 830, 971, 1890
MaeII ACGT 2 cut(s) 30, 894
MaeIII GTNAC 7 cut(s) 452, 701, 890, 1539, 2093, 2542, 2736
MalI GATC 6 cut(s) 114, 767, 1674, 2028, 2454, 2703
MbiI CCGCTC 1 cut(s) 607
MboI GATC 6 cut(s) 112, 765, 1672, 2026, 2452, 2701
MfeI CAATTG 1 cut(s) 2316
MflI RGATCY 3 cut(s) 1672, 2452, 2701
MhlI GDGCHC 2 cut(s) 81, 2446
MlyI GAGTC 6 cut(s) 1208, 2101, 2435, 2645, 2787, 2939
MmeI TCCRAC 2 cut(s) 2673, 2846
Mph1103I ATGCAT 2 cut(s) 487, 1167
MroXI GAANNNNTTC 2 cut(s) 675, 1637
MslI CAYNNNNRTG 3 cut(s) 1018, 1294, 2078
MspA1I CMGCKG 3 cut(s) 329, 1768, 2585
MspI CCGG 3 cut(s) 10, 377, 2927
MspR9I CCNGG 4 cut(s) 378, 641, 699, 2219
MunI CAATTG 1 cut(s) 2316
Mva1269I GAATGC 2 cut(s) 1099, 2802
MvaI CCWGG 3 cut(s) 641, 699, 2219
MvnI CGCG 1 cut(s) 1768
MwoI GCNNNNNNNGC 2 cut(s) 329, 1895
NciI CCSGG 1 cut(s) 378
NcoI CCATGG 3 cut(s) 600, 753, 811
NdeII GATC 6 cut(s) 112, 765, 1672, 2026, 2452, 2701
NlaIV GGNNCC 8 cut(s) 375, 547, 907, 940, 1402, 1593, 2216, 2478
NmuCI GTSAC 4 cut(s) 701, 890, 1539, 2093
NsiI ATGCAT 2 cut(s) 487, 1167
NspI RCATGY 3 cut(s) 406, 1391, 2162
PaeR7I CTCGAG 1 cut(s) 80
PceI AGGCCT 1 cut(s) 2235
PciI ACATGT 1 cut(s) 1387
PcsI WCGNNNNNNNCGW 1 cut(s) 64
PctI GAATGC 2 cut(s) 1099, 2802
PdmI GAANNNNTTC 2 cut(s) 675, 1637
PfeI GAWTC 9 cut(s) 128, 305, 626, 717, 848, 1430, 1990, 2133, 2392
PflFI GACNNNGTC 1 cut(s) 29
PkrI GCNGC 9 cut(s) 325, 328, 331, 353, 665, 1141, 1356, 2511, 2959
PleI GAGTC 6 cut(s) 1208, 2100, 2434, 2645, 2787, 2938
PpsI GAGTC 6 cut(s) 1208, 2100, 2434, 2645, 2787, 2938
PpuMI RGGWCCY 4 cut(s) 905, 938, 1401, 1591
PscI ACATGT 1 cut(s) 1387
PshBI ATTAAT 1 cut(s) 71
PsiI TTATAA 1 cut(s) 2630
Psp124BI GAGCTC 1 cut(s) 81
Psp5II RGGWCCY 4 cut(s) 905, 938, 1401, 1591
Psp6I CCWGG 3 cut(s) 639, 697, 2217
PspEI GGTNACC 1 cut(s) 701
PspFI CCCAGC 1 cut(s) 2672
PspGI CCWGG 3 cut(s) 639, 697, 2217
PspN4I GGNNCC 8 cut(s) 375, 547, 907, 940, 1402, 1593, 2216, 2478
PspPI GGNCC 6 cut(s) 905, 938, 1401, 1591, 2214, 2534
PspPPI RGGWCCY 4 cut(s) 905, 938, 1401, 1591
PstI CTGCAG 1 cut(s) 1317
PsuI RGATCY 3 cut(s) 1672, 2452, 2701
PsyI GACNNNGTC 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 2585
RsaI GTAC 9 cut(s) 65, 421, 615, 797, 861, 1739, 2304, 2591, 2831
RsaNI GTAC 9 cut(s) 64, 420, 614, 796, 860, 1738, 2303, 2590, 2830
RseI CAYNNNNRTG 3 cut(s) 1018, 1294, 2078
SacI GAGCTC 1 cut(s) 81
SacII CCGCGG 1 cut(s) 1769
SatI GCNGC 9 cut(s) 324, 327, 330, 352, 664, 1140, 1355, 2510, 2958
Sau3AI GATC 6 cut(s) 112, 765, 1672, 2026, 2452, 2701
Sau96I GGNCC 6 cut(s) 905, 938, 1401, 1591, 2214, 2534
SchI GAGTC 6 cut(s) 1208, 2101, 2435, 2645, 2787, 2939
ScrFI CCNGG 4 cut(s) 378, 641, 699, 2219
SduI GDGCHC 2 cut(s) 81, 2446
SexAI ACCWGGT 2 cut(s) 639, 697
SfaNI GCATC 7 cut(s) 646, 1152, 1174, 1285, 1627, 1797, 2882
SfcI CTRYAG 3 cut(s) 621, 1313, 1683
Sfr274I CTCGAG 1 cut(s) 80
Sfr303I CCGCGG 1 cut(s) 1769
SgrBI CCGCGG 1 cut(s) 1769
SinI GGWCC 5 cut(s) 905, 938, 1401, 1591, 2214
SlaI CTCGAG 1 cut(s) 80
SmiMI CAYNNNNRTG 3 cut(s) 1018, 1294, 2078
SmlI CTYRAG 5 cut(s) 80, 539, 863, 2107, 2795
SmoI CTYRAG 5 cut(s) 80, 539, 863, 2107, 2795
SseBI AGGCCT 1 cut(s) 2235
SsiI CCGC 8 cut(s) 329, 607, 664, 775, 932, 1766, 1768, 1786
SspI AATATT 1 cut(s) 786
SspMI CTAG 5 cut(s) 104, 395, 830, 971, 1890
SstI GAGCTC 1 cut(s) 81
StuI AGGCCT 1 cut(s) 2235
StyD4I CCNGG 4 cut(s) 376, 639, 697, 2217
StyI CCWWGG 5 cut(s) 600, 753, 811, 1072, 1797
TaaI ACNGT 7 cut(s) 63, 452, 1530, 1955, 2175, 2340, 2524
TaiI ACGT 2 cut(s) 33, 897
TaqI TCGA 3 cut(s) 81, 1925, 2728
TatI WGTACW 5 cut(s) 419, 795, 859, 2302, 2589
TauI GCSGC 2 cut(s) 332, 666
TfiI GAWTC 9 cut(s) 128, 305, 626, 717, 848, 1430, 1990, 2133, 2392
TscAI CASTG 7 cut(s) 100, 1216, 1526, 2338, 2345, 2389, 2418
TseFI GTSAC 4 cut(s) 701, 890, 1539, 2093
TseI GCWGC 7 cut(s) 323, 326, 351, 1139, 1354, 2509, 2957
Tsp45I GTSAC 4 cut(s) 701, 890, 1539, 2093
TspGWI ACGGA 1 cut(s) 506
TspRI CASTG 7 cut(s) 100, 1216, 1526, 2338, 2345, 2389, 2418
Tth111I GACNNNGTC 1 cut(s) 29
VpaK11BI GGWCC 5 cut(s) 905, 938, 1401, 1591, 2214
VspI ATTAAT 1 cut(s) 71
XapI RAATTY 6 cut(s) 425, 923, 1334, 2248, 2293, 2407
XbaI TCTAGA 1 cut(s) 394
XceI RCATGY 3 cut(s) 406, 1391, 2162
XcmI CCANNNNNNNNNTGG 1 cut(s) 140
XhoI CTCGAG 1 cut(s) 80
XmiI GTMKAC 4 cut(s) 1254, 1708, 1950, 2223
XmnI GAANNNNTTC 2 cut(s) 675, 1637
XspI CTAG 5 cut(s) 104, 395, 830, 971, 1890
ZraI GACGTC 2 cut(s) 31, 895
Zsp2I ATGCAT 2 cut(s) 487, 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.