Rorug05G0420300

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000891
Physical Location & Seq
Forward (+)
58440462 .. 58441306
845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0420300.1

Sequence Viewer

Length: 498 bp
ATGGCGAGCCTCCTCCACTTCTTCCTCCTCGCCTTCCTCCTACTCCAATTCACTGCTTCTTCCTCTTCACCAACTCCATCTCCCCAGCCAGATTCCGATTCCCCATCTCCCTCCTCCGATTCACCTTCACCCTCCAATGCCCCGCAGATTTCACTCCCGCCCTCTCCCTCCGACGCTCCGGCGAACTCGCCGAATTCCGCTTCACCTCCTGCCCCGCCGGCCTCCGATTCTCCCTCGCCGCTCCCGGATTCCGCTTCCGATGCTCCCGCGCCGGCGCCGGGTGCCAGCGACGTCAACCACAGCGATATCAACACTGACGGAGGACCTAAAACGGCGTCGTCCGACGGAGGGATGAGTGCGGGGAAGAAGGCCGGGATCGGACTCGGAGTCATCGTCGGGGTTGGGTTGGTCGGACTCGGAGCGTTGGTGTACAAGAGGCGCCAGGATAACATTCGCAGATCTCAGTACGGTTACGCCGCCAGGAGAGAGATGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

165

Amino Acids

16.55

Weight (kDa)

5.08

Isoelectric Point (pI)

103.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 294
AccB1I GGYRCC 3 cut(s) 274, 281, 438
AccBSI CCGCTC 1 cut(s) 241
AccII CGCG 1 cut(s) 269
AciI CCGC 9 cut(s) 143, 158, 198, 215, 239, 252, 267, 359, 477
AclWI GGATC 1 cut(s) 383
AcsI RAATTY 1 cut(s) 193
AcyI GRCGYC 4 cut(s) 275, 291, 335, 439
AfaI GTAC 2 cut(s) 431, 467
AfiI CCNNNNNNNGG 2 cut(s) 278, 348
AhdI GACNNNNNGTC 1 cut(s) 386
AjnI CCWGG 2 cut(s) 441, 479
AlwI GGATC 1 cut(s) 383
AoxI GGCC 2 cut(s) 219, 369
ApoI RAATTY 1 cut(s) 193
AspLEI GCGC 3 cut(s) 271, 277, 441
AspS9I GGNCC 1 cut(s) 323
AsuC2I CCSGG 3 cut(s) 245, 279, 373
AsuHPI GGTGA 4 cut(s) 60, 114, 120, 195
AvaII GGWCC 1 cut(s) 323
BanI GGYRCC 3 cut(s) 274, 281, 438
BccI CCATC 2 cut(s) 85, 112
BceAI ACGGC 1 cut(s) 348
BciT130I CCWGG 2 cut(s) 443, 481
BcnI CCSGG 3 cut(s) 245, 279, 373
BfoI RGCGCY 2 cut(s) 278, 442
BglI GCCNNNNNGGC 1 cut(s) 218
BglII AGATCT 1 cut(s) 458
BisI GCNGC 2 cut(s) 239, 477
BlsI GCNGC 2 cut(s) 240, 478
Bme1390I CCNGG 5 cut(s) 245, 279, 373, 443, 481
Bme18I GGWCC 1 cut(s) 323
BmeRI GACNNNNNGTC 1 cut(s) 386
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 3 cut(s) 276, 283, 440
BmrFI CCNGG 5 cut(s) 245, 279, 373, 443, 481
BmsI GCATC 2 cut(s) 250, 480
BplI GAGNNNNNCTC 1 cut(s) 477
BpuMI CCSGG 3 cut(s) 245, 279, 373
BsaHI GRCGYC 4 cut(s) 275, 291, 335, 439
BsaXI ACNNNNNCTCC 6 cut(s) 64, 94, 378, 408, 411, 441
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 348
Bse118I RCCGGY 2 cut(s) 217, 271
BseBI CCWGG 2 cut(s) 443, 481
BseGI GGATG 1 cut(s) 357
BseLI CCNNNNNNNGG 2 cut(s) 278, 348
BseMII CTCAG 1 cut(s) 476
BseRI GAGGAG 2 cut(s) 17, 103
BseYI CCCAGC 1 cut(s) 84
Bsh1236I CGCG 1 cut(s) 269
BshFI GGCC 2 cut(s) 221, 371
BshNI GGYRCC 3 cut(s) 274, 281, 438
BsiSI CCGG 6 cut(s) 179, 218, 245, 272, 278, 372
BslI CCNNNNNNNGG 2 cut(s) 278, 348
BsnI GGCC 2 cut(s) 221, 371
Bsp1407I TGTACA 1 cut(s) 429
Bsp143I GATC 2 cut(s) 375, 458
BspACI CCGC 9 cut(s) 143, 158, 198, 215, 239, 252, 267, 359, 477
BspANI GGCC 2 cut(s) 221, 371
BspCNI CTCAG 1 cut(s) 475
BspFNI CGCG 1 cut(s) 269
BspLI GGNNCC 3 cut(s) 276, 283, 440
BspPI GGATC 1 cut(s) 383
BspT107I GGYRCC 3 cut(s) 274, 281, 438
BsrBI CCGCTC 1 cut(s) 241
BsrFI RCCGGY 2 cut(s) 217, 271
BsrGI TGTACA 1 cut(s) 429
BssAI RCCGGY 2 cut(s) 217, 271
BssMI GATC 2 cut(s) 375, 458
BssNI GRCGYC 4 cut(s) 275, 291, 335, 439
Bst2UI CCWGG 2 cut(s) 443, 481
Bst4CI ACNGT 1 cut(s) 470
Bst6I CTCTTC 1 cut(s) 70
BstACI GRCGYC 4 cut(s) 275, 291, 335, 439
BstAUI TGTACA 1 cut(s) 429
BstC8I GCNNGC 4 cut(s) 7, 219, 273, 286
BstDEI CTNAG 1 cut(s) 462
BstF5I GGATG 1 cut(s) 357
BstFNI CGCG 1 cut(s) 269
BstH2I RGCGCY 2 cut(s) 278, 442
BstHHI GCGC 3 cut(s) 271, 277, 441
BstKTI GATC 2 cut(s) 378, 461
BstMBI GATC 2 cut(s) 375, 458
BstMWI GCNNNNNNNGC 3 cut(s) 218, 260, 281
BstNI CCWGG 2 cut(s) 443, 481
BstSCI CCNGG 5 cut(s) 243, 277, 371, 441, 479
BstUI CGCG 1 cut(s) 269
BstX2I RGATCY 1 cut(s) 458
BstYI RGATCY 1 cut(s) 458
BsuRI GGCC 2 cut(s) 221, 371
BtsCI GGATG 1 cut(s) 357
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 2 cut(s) 51, 312
Cac8I GCNNGC 4 cut(s) 7, 219, 273, 286
CfoI GCGC 3 cut(s) 271, 277, 441
Cfr10I RCCGGY 2 cut(s) 217, 271
Cfr13I GGNCC 1 cut(s) 323
CseI GACGC 2 cut(s) 182, 324
Csp6I GTAC 2 cut(s) 430, 466
CviJI RGCY 4 cut(s) 9, 88, 221, 371
CviKI_1 RGCY 4 cut(s) 9, 88, 221, 371
CviQI GTAC 2 cut(s) 430, 466
DdeI CTNAG 1 cut(s) 462
DinI GGCGCC 2 cut(s) 276, 440
DpnI GATC 2 cut(s) 377, 460
DpnII GATC 2 cut(s) 375, 458
DriI GACNNNNNGTC 1 cut(s) 386
Eam1104I CTCTTC 1 cut(s) 70
Eam1105I GACNNNNNGTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 70
Eco32I GATATC 1 cut(s) 307
Eco47I GGWCC 1 cut(s) 323
EcoO109I RGGNCCY 1 cut(s) 323
EcoRI GAATTC 1 cut(s) 193
EcoRII CCWGG 2 cut(s) 441, 479
EcoRV GATATC 1 cut(s) 307
EgeI GGCGCC 2 cut(s) 276, 440
EheI GGCGCC 2 cut(s) 276, 440
FauI CCCGC 5 cut(s) 150, 165, 222, 274, 352
Fnu4HI GCNGC 2 cut(s) 239, 477
FokI GGATG 1 cut(s) 364
Fsp4HI GCNGC 2 cut(s) 239, 477
GlaI GCGC 3 cut(s) 270, 276, 440
GluI GCNGC 2 cut(s) 239, 477
GsaI CCCAGC 1 cut(s) 88
HaeII RGCGCY 2 cut(s) 278, 442
HaeIII GGCC 2 cut(s) 221, 371
HapII CCGG 6 cut(s) 179, 218, 245, 272, 278, 372
HgaI GACGC 2 cut(s) 182, 324
HhaI GCGC 3 cut(s) 271, 277, 441
Hin1I GRCGYC 4 cut(s) 275, 291, 335, 439
Hin6I GCGC 3 cut(s) 269, 275, 439
HinP1I GCGC 3 cut(s) 269, 275, 439
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 8 cut(s) 92, 98, 119, 227, 248, 381, 387, 414
HpaII CCGG 6 cut(s) 179, 218, 245, 272, 278, 372
HphI GGTGA 4 cut(s) 60, 114, 120, 195
Hpy166II GTNNAC 2 cut(s) 295, 430
Hpy8I GTNNAC 2 cut(s) 295, 430
Hpy99I CGWCG 5 cut(s) 176, 293, 340, 347, 398
HpyAV CCTTC 3 cut(s) 43, 135, 361
HpyCH4III ACNGT 1 cut(s) 470
HpyCH4IV ACGT 1 cut(s) 291
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 260, 281
HpyF3I CTNAG 1 cut(s) 462
HpySE526I ACGT 1 cut(s) 291
Hsp92I GRCGYC 4 cut(s) 275, 291, 335, 439
HspAI GCGC 3 cut(s) 269, 275, 439
KasI GGCGCC 2 cut(s) 274, 438
KroI GCCGGC 2 cut(s) 217, 271
KroNI GCCGGC 2 cut(s) 219, 273
Kzo9I GATC 2 cut(s) 375, 458
LmnI GCTCC 4 cut(s) 181, 246, 268, 419
LweI GCATC 2 cut(s) 250, 480
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 1 cut(s) 470
MalI GATC 2 cut(s) 377, 460
MbiI CCGCTC 1 cut(s) 241
MboI GATC 2 cut(s) 375, 458
MboII GAAGA 4 cut(s) 13, 51, 57, 376
MflI RGATCY 1 cut(s) 458
MluCI AATT 2 cut(s) 47, 193
Mly113I GGCGCC 2 cut(s) 275, 439
MlyI GAGTC 3 cut(s) 375, 396, 408
MmeI TCCRAC 3 cut(s) 195, 366, 391
MreI CGCCGGCG 1 cut(s) 271
MroNI GCCGGC 2 cut(s) 217, 271
MspI CCGG 6 cut(s) 179, 218, 245, 272, 278, 372
MspR9I CCNGG 5 cut(s) 245, 279, 373, 443, 481
MvaI CCWGG 2 cut(s) 443, 481
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 3 cut(s) 218, 260, 281
NaeI GCCGGC 2 cut(s) 219, 273
NarI GGCGCC 2 cut(s) 275, 439
NciI CCSGG 3 cut(s) 245, 279, 373
NdeII GATC 2 cut(s) 375, 458
NgoMIV GCCGGC 2 cut(s) 217, 271
NlaIV GGNNCC 3 cut(s) 276, 283, 440
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PdiI GCCGGC 2 cut(s) 219, 273
PfeI GAWTC 5 cut(s) 92, 98, 119, 227, 248
PfoI TCCNGGA 1 cut(s) 243
PkrI GCNGC 2 cut(s) 240, 478
PleI GAGTC 3 cut(s) 375, 395, 408
PluTI GGCGCC 2 cut(s) 278, 442
PpsI GAGTC 3 cut(s) 375, 395, 408
PpuMI RGGWCCY 1 cut(s) 323
Psp5II RGGWCCY 1 cut(s) 323
Psp6I CCWGG 2 cut(s) 441, 479
PspFI CCCAGC 1 cut(s) 84
PspGI CCWGG 2 cut(s) 441, 479
PspN4I GGNNCC 3 cut(s) 276, 283, 440
PspPI GGNCC 1 cut(s) 323
PspPPI RGGWCCY 1 cut(s) 323
PsuI RGATCY 1 cut(s) 458
RsaI GTAC 2 cut(s) 431, 467
RsaNI GTAC 2 cut(s) 430, 466
SatI GCNGC 2 cut(s) 239, 477
Sau3AI GATC 2 cut(s) 375, 458
Sau96I GGNCC 1 cut(s) 323
SchI GAGTC 3 cut(s) 375, 396, 408
ScrFI CCNGG 5 cut(s) 245, 279, 373, 443, 481
SetI ASST 4 cut(s) 127, 208, 294, 328
SfaNI GCATC 2 cut(s) 250, 480
SfoI GGCGCC 2 cut(s) 276, 440
SgrAI CRCCGGYG 1 cut(s) 271
SinI GGWCC 1 cut(s) 323
Sse9I AATT 2 cut(s) 47, 193
SsiI CCGC 9 cut(s) 143, 158, 198, 215, 239, 252, 267, 359, 477
SspDI GGCGCC 2 cut(s) 274, 438
StyD4I CCNGG 5 cut(s) 243, 277, 371, 441, 479
TaaI ACNGT 1 cut(s) 470
TaiI ACGT 1 cut(s) 294
TasI AATT 2 cut(s) 47, 193
TatI WGTACW 1 cut(s) 429
TauI GCSGC 2 cut(s) 241, 479
TfiI GAWTC 5 cut(s) 92, 98, 119, 227, 248
TscAI CASTG 2 cut(s) 58, 319
TspGWI ACGGA 2 cut(s) 333, 360
TspRI CASTG 2 cut(s) 58, 319
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 1 cut(s) 193
ZraI GACGTC 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.