Belongs to the peptidase M16 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 414 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 440 |
| AciI | CCGC | 1 cut(s) | 187 |
| AclWI | GGATC | 2 cut(s) | 129, 206 |
| AcsI | RAATTY | 1 cut(s) | 98 |
| AfaI | GTAC | 1 cut(s) | 80 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 221, 376, 440 |
| AgsI | TTSAA | 3 cut(s) | 35, 128, 179 |
| AjnI | CCWGG | 1 cut(s) | 40 |
| AluBI | AGCT | 2 cut(s) | 115, 360 |
| AluI | AGCT | 2 cut(s) | 115, 360 |
| Alw21I | GWGCWC | 1 cut(s) | 111 |
| Alw26I | GTCTC | 1 cut(s) | 171 |
| AlwI | GGATC | 2 cut(s) | 129, 206 |
| ApeKI | GCWGC | 2 cut(s) | 335, 357 |
| ApoI | RAATTY | 1 cut(s) | 98 |
| AspS9I | GGNCC | 1 cut(s) | 45 |
| AvaII | GGWCC | 1 cut(s) | 45 |
| AxyI | CCTNAGG | 1 cut(s) | 375 |
| BanII | GRGCYC | 1 cut(s) | 248 |
| BbsI | GAAGAC | 1 cut(s) | 261 |
| Bbv12I | GWGCWC | 1 cut(s) | 111 |
| BbvI | GCAGC | 2 cut(s) | 347, 369 |
| BciT130I | CCWGG | 1 cut(s) | 42 |
| BcoDI | GTCTC | 1 cut(s) | 171 |
| BfaI | CTAG | 2 cut(s) | 203, 401 |
| BisI | GCNGC | 3 cut(s) | 187, 336, 358 |
| BlsI | GCNGC | 3 cut(s) | 188, 337, 359 |
| Bme1390I | CCNGG | 1 cut(s) | 42 |
| Bme18I | GGWCC | 1 cut(s) | 45 |
| BmgT120I | GGNCC | 1 cut(s) | 45 |
| BmiI | GGNNCC | 1 cut(s) | 46 |
| BmrFI | CCNGG | 1 cut(s) | 42 |
| BmsI | GCATC | 2 cut(s) | 22, 338 |
| BpiI | GAAGAC | 1 cut(s) | 261 |
| BpmI | CTGGAG | 1 cut(s) | 24 |
| BpuEI | CTTGAG | 2 cut(s) | 95, 153 |
| BsaJI | CCNNGG | 2 cut(s) | 41, 240 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 221, 376, 440 |
| Bse21I | CCTNAGG | 1 cut(s) | 375 |
| Bse3DI | GCAATG | 2 cut(s) | 197, 345 |
| BseBI | CCWGG | 1 cut(s) | 42 |
| BseDI | CCNNGG | 2 cut(s) | 41, 240 |
| BseGI | GGATG | 1 cut(s) | 283 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 221, 376, 440 |
| BseMI | GCAATG | 2 cut(s) | 197, 345 |
| BseMII | CTCAG | 3 cut(s) | 207, 240, 366 |
| BseXI | GCAGC | 2 cut(s) | 347, 369 |
| BsiHKAI | GWGCWC | 1 cut(s) | 111 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 221, 376, 440 |
| BsmAI | GTCTC | 1 cut(s) | 171 |
| Bsp1286I | GDGCHC | 2 cut(s) | 111, 248 |
| Bsp143I | GATC | 3 cut(s) | 69, 121, 211 |
| BspACI | CCGC | 1 cut(s) | 187 |
| BspCNI | CTCAG | 3 cut(s) | 208, 241, 367 |
| BspLI | GGNNCC | 1 cut(s) | 46 |
| BspPI | GGATC | 2 cut(s) | 129, 206 |
| BsrDI | GCAATG | 2 cut(s) | 197, 345 |
| BssECI | CCNNGG | 2 cut(s) | 41, 240 |
| BssMI | GATC | 3 cut(s) | 69, 121, 211 |
| Bst2UI | CCWGG | 1 cut(s) | 42 |
| BstDEI | CTNAG | 4 cut(s) | 182, 216, 249, 375 |
| BstF5I | GGATG | 1 cut(s) | 283 |
| BstKTI | GATC | 3 cut(s) | 72, 124, 214 |
| BstMAI | GTCTC | 1 cut(s) | 171 |
| BstMBI | GATC | 3 cut(s) | 69, 121, 211 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 335 |
| BstNI | CCWGG | 1 cut(s) | 42 |
| BstSCI | CCNGG | 1 cut(s) | 40 |
| BstV1I | GCAGC | 2 cut(s) | 347, 369 |
| BstV2I | GAAGAC | 1 cut(s) | 261 |
| BstX2I | RGATCY | 1 cut(s) | 121 |
| BstYI | RGATCY | 1 cut(s) | 121 |
| Bsu36I | CCTNAGG | 1 cut(s) | 375 |
| BtsCI | GGATG | 1 cut(s) | 283 |
| BtsI | GCAGTG | 1 cut(s) | 192 |
| BtsIMutI | CAGTG | 1 cut(s) | 192 |
| Cfr13I | GGNCC | 1 cut(s) | 45 |
| Csp6I | GTAC | 1 cut(s) | 79 |
| CviAII | CATG | 1 cut(s) | 28 |
| CviJI | RGCY | 6 cut(s) | 90, 115, 131, 186, 246, 360 |
| CviKI_1 | RGCY | 6 cut(s) | 90, 115, 131, 186, 246, 360 |
| CviQI | GTAC | 1 cut(s) | 79 |
| DdeI | CTNAG | 4 cut(s) | 182, 216, 249, 375 |
| DpnI | GATC | 3 cut(s) | 71, 123, 213 |
| DpnII | GATC | 3 cut(s) | 69, 121, 211 |
| Eco24I | GRGCYC | 1 cut(s) | 248 |
| Eco47I | GGWCC | 1 cut(s) | 45 |
| Eco81I | CCTNAGG | 1 cut(s) | 375 |
| EcoRII | CCWGG | 1 cut(s) | 40 |
| EcoT38I | GRGCYC | 1 cut(s) | 248 |
| FaeI | CATG | 1 cut(s) | 31 |
| FaiI | YATR | 5 cut(s) | 29, 68, 414, 424, 426 |
| FatI | CATG | 1 cut(s) | 27 |
| FauNDI | CATATG | 1 cut(s) | 424 |
| Fnu4HI | GCNGC | 3 cut(s) | 187, 336, 358 |
| FokI | GGATG | 1 cut(s) | 290 |
| FriOI | GRGCYC | 1 cut(s) | 248 |
| Fsp4HI | GCNGC | 3 cut(s) | 187, 336, 358 |
| FspBI | CTAG | 2 cut(s) | 203, 401 |
| GluI | GCNGC | 3 cut(s) | 187, 336, 358 |
| GsuI | CTGGAG | 1 cut(s) | 24 |
| Hin1II | CATG | 1 cut(s) | 31 |
| HinfI | GANTC | 1 cut(s) | 311 |
| Hpy166II | GTNNAC | 2 cut(s) | 48, 208 |
| Hpy188I | TCNGA | 4 cut(s) | 241, 250, 318, 367 |
| Hpy188III | TCNNGA | 5 cut(s) | 125, 139, 209, 215, 374 |
| Hpy8I | GTNNAC | 2 cut(s) | 48, 208 |
| Hpy99I | CGWCG | 1 cut(s) | 263 |
| HpyAV | CCTTC | 2 cut(s) | 162, 263 |
| HpyCH4IV | ACGT | 1 cut(s) | 81 |
| HpyCH4V | TGCA | 5 cut(s) | 13, 76, 197, 392, 422 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 335 |
| HpyF3I | CTNAG | 4 cut(s) | 182, 216, 249, 375 |
| HpySE526I | ACGT | 1 cut(s) | 81 |
| Hsp92II | CATG | 1 cut(s) | 31 |
| Kzo9I | GATC | 3 cut(s) | 69, 121, 211 |
| LmnI | GCTCC | 1 cut(s) | 24 |
| LpnPI | CCDG | 5 cut(s) | 27, 54, 228, 368, 387 |
| Lsp1109I | GCAGC | 2 cut(s) | 347, 369 |
| LweI | GCATC | 2 cut(s) | 22, 338 |
| MaeI | CTAG | 2 cut(s) | 203, 401 |
| MaeII | ACGT | 1 cut(s) | 81 |
| MalI | GATC | 3 cut(s) | 71, 123, 213 |
| MboI | GATC | 3 cut(s) | 69, 121, 211 |
| MboII | GAAGA | 2 cut(s) | 266, 293 |
| MfeI | CAATTG | 1 cut(s) | 19 |
| MflI | RGATCY | 1 cut(s) | 121 |
| MhlI | GDGCHC | 2 cut(s) | 111, 248 |
| MluCI | AATT | 2 cut(s) | 19, 98 |
| MnlI | CCTC | 8 cut(s) | 48, 200, 235, 244, 268, 299, 370, 381 |
| MseI | TTAA | 1 cut(s) | 445 |
| MspR9I | CCNGG | 1 cut(s) | 42 |
| MunI | CAATTG | 1 cut(s) | 19 |
| MvaI | CCWGG | 1 cut(s) | 42 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 335 |
| NdeI | CATATG | 1 cut(s) | 424 |
| NdeII | GATC | 3 cut(s) | 69, 121, 211 |
| NlaIII | CATG | 1 cut(s) | 31 |
| NlaIV | GGNNCC | 1 cut(s) | 46 |
| PfeI | GAWTC | 1 cut(s) | 311 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 440 |
| PkrI | GCNGC | 3 cut(s) | 188, 337, 359 |
| PsiI | TTATAA | 1 cut(s) | 414 |
| Psp6I | CCWGG | 1 cut(s) | 40 |
| PspGI | CCWGG | 1 cut(s) | 40 |
| PspN4I | GGNNCC | 1 cut(s) | 46 |
| PspPI | GGNCC | 1 cut(s) | 45 |
| PsuI | RGATCY | 1 cut(s) | 121 |
| RsaI | GTAC | 1 cut(s) | 80 |
| RsaNI | GTAC | 1 cut(s) | 79 |
| SaqAI | TTAA | 1 cut(s) | 445 |
| SatI | GCNGC | 3 cut(s) | 187, 336, 358 |
| Sau3AI | GATC | 3 cut(s) | 69, 121, 211 |
| Sau96I | GGNCC | 1 cut(s) | 45 |
| ScrFI | CCNGG | 1 cut(s) | 42 |
| SduI | GDGCHC | 2 cut(s) | 111, 248 |
| SetI | ASST | 5 cut(s) | 40, 84, 117, 362, 406 |
| SfaNI | GCATC | 2 cut(s) | 22, 338 |
| SinI | GGWCC | 1 cut(s) | 45 |
| SmlI | CTYRAG | 2 cut(s) | 110, 168 |
| SmoI | CTYRAG | 2 cut(s) | 110, 168 |
| Sse9I | AATT | 2 cut(s) | 19, 98 |
| SsiI | CCGC | 1 cut(s) | 187 |
| SspMI | CTAG | 2 cut(s) | 203, 401 |
| StyD4I | CCNGG | 1 cut(s) | 40 |
| TaiI | ACGT | 1 cut(s) | 84 |
| TasI | AATT | 2 cut(s) | 19, 98 |
| TauI | GCSGC | 1 cut(s) | 189 |
| TfiI | GAWTC | 1 cut(s) | 311 |
| Tru1I | TTAA | 1 cut(s) | 445 |
| Tru9I | TTAA | 1 cut(s) | 445 |
| TscAI | CASTG | 1 cut(s) | 199 |
| TseI | GCWGC | 2 cut(s) | 335, 357 |
| TspDTI | ATGAA | 2 cut(s) | 294, 300 |
| TspRI | CASTG | 1 cut(s) | 199 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 440 |
| VpaK11BI | GGWCC | 1 cut(s) | 45 |
| XapI | RAATTY | 1 cut(s) | 98 |
| XspI | CTAG | 2 cut(s) | 203, 401 |