RchiOBHm_Chr5g0074171

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000891
Physical Location & Seq
Reverse (-)
80172232 .. 80175081
2850 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34900

Sequence Viewer

Length: 567 bp
ATGGTGCACTATCCTGAGATTGGAGATTCCCAACTCTCCGAGGGCTCTGAGGAAGCTGAGGAAGAAGAAGGAATGGAGGAGGAGGATGAGGAAGACGAAGATTGTGAAGGAGAGGATGAAGATGATGAGGGAGAAGATGAAGATGAACTGAAGAAGAAAAAGGGAGGGGATTCTCAGACTAAGAAGGTTGCAATGTGTGCAGGAATAGGCAGCTTCTGTGACCCTCCTGAGGCACAGGGGCTTGCACACTTTTTAGAACACATGCTCTTTATGGGGAGTACAGAATTTGCAGATGAGAAGGAGTATGACAGTTACTTGTCCAAGCACGGAGGGTGTTTAAATGCATACACAGAAGTAGAGCATACTTGCTACCATTTTGAAGTGAAACGAGAGTTTCTCAAGGGTGCCTTGACAAGATTTTCTCAGTTCTTTGTTTCACCCCTTGTAAAAATTGAAGCCATGGAGCGGGAGTTACAGGCTGTAGATTCAGGTGCATGTTACTCTTGTCTGAGTTTATTGATATCAGTCTTTCCTTTTAAGAAATGTCCCAGTTTTGCTAAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

188

Amino Acids

21.15

Weight (kDa)

4.31

Isoelectric Point (pI)

59.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 59 - 162 2.3e-27 Insulinase (Peptidase family M16)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 404
AccBSI CCGCTC 1 cut(s) 466
AciI CCGC 1 cut(s) 466
AcsI RAATTY 1 cut(s) 284
AcuI CTGAAG 1 cut(s) 170
AfaI GTAC 1 cut(s) 280
AfiI CCNNNNNNNGG 3 cut(s) 20, 229, 465
AgsI TTSAA 2 cut(s) 380, 455
AluBI AGCT 2 cut(s) 56, 213
AluI AGCT 2 cut(s) 56, 213
Alw21I GWGCWC 1 cut(s) 9
Alw44I GTGCAC 1 cut(s) 5
AlwNI CAGNNNCTG 1 cut(s) 216
ApaLI GTGCAC 1 cut(s) 5
ApeKI GCWGC 1 cut(s) 210
ApoI RAATTY 1 cut(s) 284
AsuHPI GGTGA 1 cut(s) 429
AxyI CCTNAGG 1 cut(s) 228
BaeGI GKGCMC 1 cut(s) 9
BanI GGYRCC 1 cut(s) 404
BanII GRGCYC 1 cut(s) 47
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 9
BbvCI CCTCAGC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 222
BfmI CTRYAG 1 cut(s) 480
BisI GCNGC 1 cut(s) 211
BlsI GCNGC 1 cut(s) 212
BmiI GGNNCC 1 cut(s) 406
BmrI ACTGGG 1 cut(s) 543
BmuI ACTGGG 1 cut(s) 543
BpiI GAAGAC 1 cut(s) 99
BplI GAGNNNNNCTC 2 cut(s) 381, 413
Bpu10I CCTNAGC 1 cut(s) 57
BpuEI CTTGAG 1 cut(s) 383
BsaJI CCNNGG 2 cut(s) 39, 459
BsaXI ACNNNNNCTCC 2 cut(s) 455, 485
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 229, 465
Bse1I ACTGG 1 cut(s) 549
Bse21I CCTNAGG 1 cut(s) 228
Bse3DI GCAATG 1 cut(s) 198
BseDI CCNNGG 2 cut(s) 39, 459
BseGI GGATG 2 cut(s) 91, 121
BseLI CCNNNNNNNGG 3 cut(s) 20, 229, 465
BseMI GCAATG 1 cut(s) 198
BseMII CTCAG 7 cut(s) 6, 39, 48, 188, 219, 437, 500
BseNI ACTGG 1 cut(s) 549
BseRI GAGGAG 2 cut(s) 92, 95
BseSI GKGCMC 1 cut(s) 9
BseXI GCAGC 1 cut(s) 222
BsgI GTGCAG 1 cut(s) 219
BshNI GGYRCC 1 cut(s) 404
BsiHKAI GWGCWC 1 cut(s) 9
BslFI GGGAC 1 cut(s) 531
BslI CCNNNNNNNGG 3 cut(s) 20, 229, 465
BsmFI GGGAC 1 cut(s) 531
Bsp1286I GDGCHC 2 cut(s) 9, 47
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 1 cut(s) 466
BspCNI CTCAG 7 cut(s) 7, 40, 49, 187, 220, 436, 501
BspLI GGNNCC 1 cut(s) 406
BspT107I GGYRCC 1 cut(s) 404
BsrBI CCGCTC 1 cut(s) 466
BsrDI GCAATG 1 cut(s) 198
BsrI ACTGG 1 cut(s) 549
BssECI CCNNGG 2 cut(s) 39, 459
BssT1I CCWWGG 1 cut(s) 459
Bst4CI ACNGT 1 cut(s) 311
BstAPI GCANNNNNTGC 1 cut(s) 197
BstC8I GCNNGC 1 cut(s) 243
BstDEI CTNAG 8 cut(s) 15, 48, 57, 174, 180, 228, 423, 509
BstDSI CCRYGG 1 cut(s) 459
BstF5I GGATG 2 cut(s) 91, 121
BstMWI GCNNNNNNNGC 1 cut(s) 197
BstNSI RCATGY 2 cut(s) 265, 498
BstSFI CTRYAG 1 cut(s) 480
BstSLI GKGCMC 1 cut(s) 9
BstV1I GCAGC 1 cut(s) 222
BstV2I GAAGAC 1 cut(s) 99
Bsu36I CCTNAGG 1 cut(s) 228
BtgI CCRYGG 1 cut(s) 459
BtsCI GGATG 2 cut(s) 91, 121
Cac8I GCNNGC 1 cut(s) 243
CaiI CAGNNNCTG 1 cut(s) 216
Csp6I GTAC 1 cut(s) 279
CviAII CATG 3 cut(s) 262, 460, 495
CviJI RGCY 6 cut(s) 45, 56, 213, 241, 458, 479
CviKI_1 RGCY 6 cut(s) 45, 56, 213, 241, 458, 479
CviQI GTAC 1 cut(s) 279
DdeI CTNAG 8 cut(s) 15, 48, 57, 174, 180, 228, 423, 509
DraI TTTAAA 1 cut(s) 339
Eco130I CCWWGG 1 cut(s) 459
Eco24I GRGCYC 1 cut(s) 47
Eco32I GATATC 1 cut(s) 522
Eco57I CTGAAG 1 cut(s) 170
Eco81I CCTNAGG 1 cut(s) 228
EcoRV GATATC 1 cut(s) 522
EcoT14I CCWWGG 1 cut(s) 459
EcoT22I ATGCAT 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 47
ErhI CCWWGG 1 cut(s) 459
FaeI CATG 3 cut(s) 265, 463, 498
FaiI YATR 7 cut(s) 263, 272, 306, 346, 363, 461, 496
FalI AAGNNNNNCTT 2 cut(s) 392, 424
FaqI GGGAC 1 cut(s) 531
FatI CATG 3 cut(s) 261, 459, 494
FauI CCCGC 1 cut(s) 459
Fnu4HI GCNGC 1 cut(s) 211
FokI GGATG 2 cut(s) 98, 128
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 1 cut(s) 211
GluI GCNGC 1 cut(s) 211
Hin1II CATG 3 cut(s) 265, 463, 498
HinfI GANTC 3 cut(s) 26, 170, 485
HphI GGTGA 1 cut(s) 429
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 4 cut(s) 40, 49, 177, 510
Hpy188III TCNNGA 2 cut(s) 14, 227
Hpy8I GTNNAC 1 cut(s) 7
HpyAV CCTTC 4 cut(s) 62, 101, 178, 292
HpyCH4III ACNGT 1 cut(s) 311
HpyCH4V TGCA 7 cut(s) 7, 191, 200, 245, 290, 344, 494
HpyF10VI GCNNNNNNNGC 1 cut(s) 197
HpyF3I CTNAG 8 cut(s) 15, 48, 57, 174, 180, 228, 423, 509
Hsp92II CATG 3 cut(s) 265, 463, 498
LmnI GCTCC 1 cut(s) 463
LpnPI CCDG 7 cut(s) 27, 186, 221, 240, 461, 474, 562
Lsp1109I GCAGC 1 cut(s) 222
MaeIII GTNAC 4 cut(s) 218, 311, 471, 497
MbiI CCGCTC 1 cut(s) 466
MboII GAAGA 9 cut(s) 74, 77, 104, 110, 131, 146, 152, 163, 166
MhlI GDGCHC 2 cut(s) 9, 47
MluCI AATT 2 cut(s) 284, 450
Mph1103I ATGCAT 1 cut(s) 346
MseI TTAA 2 cut(s) 338, 537
MwoI GCNNNNNNNGC 1 cut(s) 197
NcoI CCATGG 1 cut(s) 459
NlaIII CATG 3 cut(s) 265, 463, 498
NlaIV GGNNCC 1 cut(s) 406
NmuCI GTSAC 1 cut(s) 218
NsiI ATGCAT 1 cut(s) 346
NspI RCATGY 2 cut(s) 265, 498
PfeI GAWTC 3 cut(s) 26, 170, 485
PkrI GCNGC 1 cut(s) 212
PspN4I GGNNCC 1 cut(s) 406
PstNI CAGNNNCTG 1 cut(s) 216
RsaI GTAC 1 cut(s) 280
RsaNI GTAC 1 cut(s) 279
SaqAI TTAA 2 cut(s) 338, 537
SatI GCNGC 1 cut(s) 211
SduI GDGCHC 2 cut(s) 9, 47
SetI ASST 4 cut(s) 58, 189, 215, 493
SfcI CTRYAG 1 cut(s) 480
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 2 cut(s) 284, 450
SsiI CCGC 1 cut(s) 466
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 1 cut(s) 311
TasI AATT 2 cut(s) 284, 450
TatI WGTACW 1 cut(s) 278
TfiI GAWTC 3 cut(s) 26, 170, 485
Tru1I TTAA 2 cut(s) 338, 537
Tru9I TTAA 2 cut(s) 338, 537
TseFI GTSAC 1 cut(s) 218
TseI GCWGC 1 cut(s) 210
Tsp45I GTSAC 1 cut(s) 218
TspDTI ATGAA 3 cut(s) 132, 153, 159
TspGWI ACGGA 1 cut(s) 342
VneI GTGCAC 1 cut(s) 5
XapI RAATTY 1 cut(s) 284
XceI RCATGY 2 cut(s) 265, 498
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.