RchiOBHm_Chr5g0072811

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000891
Physical Location & Seq
Reverse (-)
78863032 .. 78864870
1839 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34773

Sequence Viewer

Length: 519 bp
ATGGCTCGCCGGACTTTCTCATCGGACGACGTCGTTTTGAAGTCTCCGAATGATAGACGACTGTACCGATTGATCAATCTCGAGAATGGCCTCACTGCATTGCTAGTCCACGATCCTGAGATTGGAGATTCCCAACTCTCCAAGGGCTCTGAGGAAGCTGAGATGGAAGAAGAAGAAGAAGAAGAAGGAATGGAGGAGGATGAGAAAGACGACGATGAAGAAGACGAAGATAGTGAAGGAGAGGATGAAGAGATGGATGAAGATGATGAGGGAGAAGATGAAGATGGACTGAAGAAGAAAAAGGGAGGGGATTCTCAGACTAAGAAGGCAGCAGCGGCAATGTGTGTAGGAATAGGCAGCTTCTCTGACCCTCCTGAGGCACCGGGGCTTGCACACTTTTTAGAACACATGCTCTTTATGGGGAGTACAGAATTTCCAGATGAAAATGAGTTACTTGTCCAAGCATGGAGGGTGTTCAAATGCATACACGGAAGTAGAGCATACTTGCTACCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

172

Amino Acids

19.32

Weight (kDa)

4.14

Isoelectric Point (pI)

58.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 105 - 151 1.6e-12 Insulinase (Peptidase family M16)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 33
AccB1I GGYRCC 1 cut(s) 379
AciI CCGC 1 cut(s) 335
AclWI GGATC 1 cut(s) 107
AcsI RAATTY 1 cut(s) 431
AcuI CTGAAG 1 cut(s) 311
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 2 cut(s) 65, 427
AfiI CCNNNNNNNGG 2 cut(s) 122, 376
AgsI TTSAA 2 cut(s) 40, 478
AluBI AGCT 2 cut(s) 158, 360
AluI AGCT 2 cut(s) 158, 360
Alw26I GTCTC 1 cut(s) 48
AlwI GGATC 1 cut(s) 107
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 1 cut(s) 88
ApeKI GCWGC 3 cut(s) 329, 332, 357
ApoI RAATTY 1 cut(s) 431
AsuC2I CCSGG 1 cut(s) 384
AvaI CYCGRG 1 cut(s) 80
AxyI CCTNAGG 1 cut(s) 375
BaeI ACNNNNGTAYC 2 cut(s) 47, 80
BanI GGYRCC 1 cut(s) 379
BanII GRGCYC 1 cut(s) 149
BbsI GAAGAC 1 cut(s) 228
BbvI GCAGC 3 cut(s) 341, 344, 369
BccI CCATC 3 cut(s) 157, 247, 278
BclI TGATCA 1 cut(s) 72
BcnI CCSGG 1 cut(s) 384
BcoDI GTCTC 1 cut(s) 48
BfaI CTAG 1 cut(s) 104
BisI GCNGC 4 cut(s) 330, 333, 336, 358
BlsI GCNGC 4 cut(s) 331, 334, 337, 359
Bme1390I CCNGG 1 cut(s) 384
BmeT110I CYCGRG 1 cut(s) 80
BmiI GGNNCC 1 cut(s) 381
BmrFI CCNGG 1 cut(s) 384
BpiI GAAGAC 1 cut(s) 228
BpuMI CCSGG 1 cut(s) 384
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 2 cut(s) 141, 383
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 376
Bse21I CCTNAGG 1 cut(s) 375
Bse3DI GCAATG 2 cut(s) 98, 345
BseDI CCNNGG 2 cut(s) 141, 383
BseGI GGATG 3 cut(s) 205, 250, 262
BseLI CCNNNNNNNGG 2 cut(s) 122, 376
BseMI GCAATG 2 cut(s) 98, 345
BseMII CTCAG 5 cut(s) 108, 141, 150, 329, 366
BseRI GAGGAG 1 cut(s) 209
BseXI GCAGC 3 cut(s) 341, 344, 369
BshFI GGCC 1 cut(s) 90
BshNI GGYRCC 1 cut(s) 379
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 2 cut(s) 10, 383
BslI CCNNNNNNNGG 2 cut(s) 122, 376
BsmAI GTCTC 1 cut(s) 48
BsnI GGCC 1 cut(s) 90
BsoBI CYCGRG 1 cut(s) 80
Bsp1286I GDGCHC 1 cut(s) 149
Bsp143I GATC 2 cut(s) 72, 112
BspACI CCGC 1 cut(s) 335
BspANI GGCC 1 cut(s) 90
BspCNI CTCAG 5 cut(s) 109, 142, 151, 328, 367
BspLI GGNNCC 1 cut(s) 381
BspPI GGATC 1 cut(s) 107
BspT107I GGYRCC 1 cut(s) 379
BsrDI GCAATG 2 cut(s) 98, 345
BssECI CCNNGG 2 cut(s) 141, 383
BssMI GATC 2 cut(s) 72, 112
BssNI GRCGYC 1 cut(s) 30
BssT1I CCWWGG 1 cut(s) 141
Bst4CI ACNGT 1 cut(s) 63
Bst6I CTCTTC 1 cut(s) 243
BstACI GRCGYC 1 cut(s) 30
BstC8I GCNNGC 2 cut(s) 7, 390
BstDEI CTNAG 6 cut(s) 117, 150, 159, 315, 321, 375
BstF5I GGATG 3 cut(s) 205, 250, 262
BstKTI GATC 2 cut(s) 75, 115
BstMAI GTCTC 1 cut(s) 48
BstMBI GATC 2 cut(s) 72, 112
BstMWI GCNNNNNNNGC 1 cut(s) 335
BstNSI RCATGY 1 cut(s) 412
BstSCI CCNGG 1 cut(s) 382
BstV1I GCAGC 3 cut(s) 341, 344, 369
BstV2I GAAGAC 1 cut(s) 228
Bsu36I CCTNAGG 1 cut(s) 375
BsuRI GGCC 1 cut(s) 90
BtsCI GGATG 3 cut(s) 205, 250, 262
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 93
Cac8I GCNNGC 2 cut(s) 7, 390
Csp6I GTAC 2 cut(s) 64, 426
CviAII CATG 2 cut(s) 409, 465
CviJI RGCY 6 cut(s) 5, 90, 147, 158, 360, 388
CviKI_1 RGCY 6 cut(s) 5, 90, 147, 158, 360, 388
CviQI GTAC 2 cut(s) 64, 426
DdeI CTNAG 6 cut(s) 117, 150, 159, 315, 321, 375
DpnI GATC 2 cut(s) 74, 114
DpnII GATC 2 cut(s) 72, 112
Eam1104I CTCTTC 1 cut(s) 243
EarI CTCTTC 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 149
Eco57I CTGAAG 1 cut(s) 311
Eco81I CCTNAGG 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 80
EcoT14I CCWWGG 1 cut(s) 141
EcoT22I ATGCAT 1 cut(s) 485
EcoT38I GRGCYC 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 2 cut(s) 412, 468
FaiI YATR 5 cut(s) 410, 419, 466, 485, 502
FatI CATG 2 cut(s) 408, 464
FbaI TGATCA 1 cut(s) 72
Fnu4HI GCNGC 4 cut(s) 330, 333, 336, 358
FokI GGATG 3 cut(s) 212, 257, 269
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 4 cut(s) 330, 333, 336, 358
FspBI CTAG 1 cut(s) 104
GluI GCNGC 4 cut(s) 330, 333, 336, 358
HaeIII GGCC 1 cut(s) 90
HapII CCGG 2 cut(s) 10, 383
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 2 cut(s) 412, 468
HinfI GANTC 2 cut(s) 128, 311
HpaII CCGG 2 cut(s) 10, 383
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 5 cut(s) 25, 48, 151, 318, 367
Hpy188III TCNNGA 5 cut(s) 80, 82, 116, 374, 437
Hpy8I GTNNAC 1 cut(s) 109
Hpy99I CGWCG 3 cut(s) 32, 35, 215
HpyAV CCTTC 3 cut(s) 179, 230, 319
HpyCH4III ACNGT 1 cut(s) 63
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 3 cut(s) 98, 392, 483
HpyF10VI GCNNNNNNNGC 1 cut(s) 335
HpyF3I CTNAG 6 cut(s) 117, 150, 159, 315, 321, 375
HpySE526I ACGT 1 cut(s) 30
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 2 cut(s) 412, 468
Ksp22I TGATCA 1 cut(s) 72
Kzo9I GATC 2 cut(s) 72, 112
LpnPI CCDG 5 cut(s) 23, 129, 387, 396, 450
Lsp1109I GCAGC 3 cut(s) 341, 344, 369
MaeI CTAG 1 cut(s) 104
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 1 cut(s) 450
MalI GATC 2 cut(s) 74, 114
MboI GATC 2 cut(s) 72, 112
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 1 cut(s) 431
Mph1103I ATGCAT 1 cut(s) 485
MspA1I CMGCKG 1 cut(s) 335
MspI CCGG 2 cut(s) 10, 383
MspR9I CCNGG 1 cut(s) 384
MwoI GCNNNNNNNGC 1 cut(s) 335
NciI CCSGG 1 cut(s) 384
NdeII GATC 2 cut(s) 72, 112
NlaIII CATG 2 cut(s) 412, 468
NlaIV GGNNCC 1 cut(s) 381
NsiI ATGCAT 1 cut(s) 485
NspI RCATGY 1 cut(s) 412
PaeR7I CTCGAG 1 cut(s) 80
PcsI WCGNNNNNNNCGW 1 cut(s) 64
PfeI GAWTC 2 cut(s) 128, 311
PflFI GACNNNGTC 1 cut(s) 29
PkrI GCNGC 4 cut(s) 331, 334, 337, 359
PspN4I GGNNCC 1 cut(s) 381
PsyI GACNNNGTC 1 cut(s) 29
RsaI GTAC 2 cut(s) 65, 427
RsaNI GTAC 2 cut(s) 64, 426
SatI GCNGC 4 cut(s) 330, 333, 336, 358
Sau3AI GATC 2 cut(s) 72, 112
ScrFI CCNGG 1 cut(s) 384
SduI GDGCHC 1 cut(s) 149
SetI ASST 3 cut(s) 33, 160, 362
Sfr274I CTCGAG 1 cut(s) 80
SlaI CTCGAG 1 cut(s) 80
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 1 cut(s) 431
SsiI CCGC 1 cut(s) 335
SspMI CTAG 1 cut(s) 104
StyD4I CCNGG 1 cut(s) 382
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 1 cut(s) 63
TaiI ACGT 1 cut(s) 33
TaqI TCGA 1 cut(s) 81
TasI AATT 1 cut(s) 431
TatI WGTACW 1 cut(s) 425
TauI GCSGC 1 cut(s) 338
TfiI GAWTC 2 cut(s) 128, 311
TscAI CASTG 1 cut(s) 100
TseI GCWGC 3 cut(s) 329, 332, 357
TspDTI ATGAA 5 cut(s) 231, 261, 273, 294, 456
TspGWI ACGGA 1 cut(s) 504
TspRI CASTG 1 cut(s) 100
Tth111I GACNNNGTC 1 cut(s) 29
XapI RAATTY 1 cut(s) 431
XceI RCATGY 1 cut(s) 412
XhoI CTCGAG 1 cut(s) 80
XspI CTAG 1 cut(s) 104
ZraI GACGTC 1 cut(s) 31
Zsp2I ATGCAT 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.