FvH4_3g40361

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000183
Physical Location & Seq
Reverse (-)
34072758 .. 34099500
26743 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g40361.t26

Sequence Viewer

Length: 3093 bp
ATGGCTCGCCGGACTTTCTCATCGGACGACGTCGTTGTGAAGTCTCCGAACGACAAACGACTCTACCGTTTAATCAAGCTCGACAACGGCCTCACCGCATTGCTAGTTCACGATCCTGAGATTGGAGAAGCCCAACTCTCCAAGGACTCTGAGCGAACTGAAGAAGCTGAGGAAGAAGACGAAGAAGAAGAGGATGAGGATGAGGATGAGGATGACGAAGACGATGAAGATGACGAAGAAGATAGTGAAGAAGATGATGAAGAGGAGGATGAGGAAGATGAAGATGAGCTGAAGAAGAAGAAGAAAAAGGGAGGGGATTCTCAGACTAAGAAGGCAGCCGCAGCAATGTGTGTAGGAATAGGCAGCTTCTCTGACCCTCCTGAGGCACAGGGGCTCGCACACTTTCTAGAGCACATGCTCTTTATGGGTAGTAAGGAATTTCCAGATGAAAACGAGTATGATAGTTACTTGTCCAAGCACGGAGGGTCCTCAAATGCCTACACAGAAGCAGAGCATACTTGCTACCATTTCGAAGTGAAACGGGAGTTTCTCAAGGGTGCCTTGACAAGATTTTCTCAGTTCTTTGTTTCACCCCTGGTAAAAAGTGAAGCCATGGAGCGGGAGGTACAGGCTGTAGATTCAGAGTTTAACCAGGTTCTGCAAAACGATGCTTGCCGCCTTGAACAACTTCAATGCCATACAGCCTCACCTGGTCACCCATTTAATAGATTCGCATGGGGGAATAAAAAGAGCTTATCTGATGCCATGGAAAAAGGGATCAATTTGCGGGAACAAATATTGAAATTGTACAGAGATTTTTACCATGGTGGATTGATGAAGCTAGTTGTCATTGGTGGAGAGTCTCTTGATGTACTTGAGAATTGGGTTTTGGAATTGTTTGGTAACGTCAAAAAGGGTCCTCAAGTAAAGCTGGAATTCAAGGCAGCGGAAGGCCCAATTTGGAAAGGTGGAAAAGTTTACAGGCTAGAGGCTGTTAAAGATATTCATATACTCCACTTAACATGGACATTTCCATGTCTTCGTCAAGATTATTTGAAGAAATCAGAAGATTATATATCTCATCTCCTTGGGCATGAGGGTAGAGGAAGTTTGCATTCCTATTTCAAAGCTAGAGGGTGGGCAACATCTCTGGCTGCTGGTGTTGGAGATGATGGGATGCATCGTTCTTCTGTGGCTTATGTATTTCGCATGGATATTTATCTCACTGACTCTGGATTGGATAAGATTTTTGATATAATTGGCTTGGTCTATCAATACATAAAGTTATTGCATAAAGTGTCCCCACAAGAATGGATATTTAAGGAGCTGCAGGATACTGGGAACATGGAATTTAGATTTGCAGAGGAGCAGCCTCAGGATGATTATGCCTCAGAACTTGCAGGAAATCTACTCATTTATGCAGCAGAGCATGTTATTTATGGTGCCTATGCCTACAAGATCTGGGCTGAAGAATCAATCAAATATGTTCTTAATTTCCTTAGACCAGAAAACATGAGGATTGATGTTGTATCAAAGCCCTCAATGAAGTTAGAAGATTTCCAGTGCGAACCTTGGTTTGGCTCACATTATACTGAAGAAGATATATCTCCATCTTTGATAGATTTGTGGAAAGATCCTCCAGAAATTGATGTTTCATTGCATCTCCCGGAAAAAAATGAGTTCATTCCTACTGATTTTTCCATTCGTTCTGATGGTCTTGATACTACAGATGTATCTTTGCCGAGATGTATACTGGATGAGCCATTGGTGAAGTTCTGGTACAAACTTGATAGTACATTTAAACTCCCACGGGCAAATACTTATTTCCGCATCAATCTAAAGGGTGGATATGATAATGTGAAGAGTTGTGTCTTGACTGAACTATATATATCCCTCCTTAAAGATGAGCTGAATGAGATTGTTTATCAGGCTAGTATGGCCAAGCTGGAAACTTCGGTATCTGTATCTTCTGACAATCTGGAGCTGAAGGTCTACGGTTTCAATGACAAGCTTCCAGCTCTGTTGTCAAAGATTCTGAAAACAACCAAAAGTTTCATGCCGACTTCTGATCGTTTTCTGGTTATTAAAGAAAATATGGAGCGAAAATTGAAGAACACCAATATGAAGCCTCTGAGTCACTCTTCATACTTGAGACTGCAAGTATTGTTCCAGAAATTCTATGATGTTGATGAGAAGCTGCATGTTTTGAATGGATTGTCTGTTTCTGATATGAAGCTATTTATTCCCCAGCTTTGTTCCCAGCTATACATTGAAGGCCTTTGTCATGGAAATTTGTCAGAAAAAGAAGCAATCAGCCTTTCAGATATATTCAAAACAAATTTTTCTGTACAACCACTTCCTGTTGAACTGAGGCATAGAGAACACTTTACTTGTCTACCGCCTTCTGCTAACCTCATAAGAGATGCCAGTGTGAAGAATAAGTCCGAAACAAATTCGGTGATTGAGCTGTATTTTCAAATTGAGCGGGAAGTGTTTAGTGAGTCCCCCAGAATGAGGGCCCTGATTGATCTTTTTGATGAAATTGTGGAGGAACCACTTTTCAATCAGCTAAGGACAAAGGAGCAACTTGGTTATACTGTTCAGTGTGCCCCGCGAGTTACCACGAACGTATTTGGATTTTGCTTTTACGTTCAGTCGGCTGAGTACAACCCAATCTACTTGCAAGGAAGACTCGAGATCTTTATAAAAAGTTTGGAGGAATTGTTGCAAGGACTAGACGATGACTCTTTTGAGAATTACAGAGCTGGGCTAATGGCAAAGCTATTGGAGAAAGATCCATCCCTTCAATATGAAACCAATCGATTTTGGTCAGAGATTACCGATAAAAGGTATATGTTTGACTATACAAAACAGGAAGCAGTACAACTCAAAAACATTCAGAAGGAGGATGTCATCAACTGGTACAAAACATATTTGCAACAATTGTCTCCCAAGTGTCGGAAACTAGCAGTTCGTGTTTGGGGATGCAACACAGACATGAAAGAGGCTGAAGCACGGCCGGAGTCTGTAAAAGTCATTGAAGACCTTGGAGCCTTTACGATGTCATCTGAGTTCTATCCTAGCAATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

1031

Amino Acids

118.79

Weight (kDa)

4.87

Isoelectric Point (pI)

43.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 78 - 192 1.2e-29 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 234 - 407 3.1e-18 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 414 - 699 1.1e-83 Middle or third domain of peptidase_M16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2705
AatII GACGTC 1 cut(s) 33
AccB1I GGYRCC 2 cut(s) 557, 1442
AccBSI CCGCTC 2 cut(s) 619, 2485
AccI GTMKAC 3 cut(s) 1750, 1992, 2395
AccII CGCG 1 cut(s) 2614
AclWI GGATC 4 cut(s) 107, 785, 1628, 2789
AcoI YGGCCR 2 cut(s) 1938, 3017
AcsI RAATTY 7 cut(s) 437, 935, 1349, 2174, 2290, 2338, 2452
AcuI CTGAAG 6 cut(s) 180, 311, 1488, 1614, 2006, 3030
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 9 cut(s) 627, 809, 873, 1781, 1795, 2349, 2666, 2883, 2924
AfiI CCNNNNNNNGG 7 cut(s) 122, 382, 618, 1577, 2514, 2847, 2958
AjnI CCWGG 3 cut(s) 594, 651, 709
AjuI GAANNNNNNNTTGG 4 cut(s) 872, 904, 1560, 1592
AloI GAACNNNNNNTCC 2 cut(s) 1168, 1200
Alw21I GWGCWC 1 cut(s) 414
Alw26I GTCTC 4 cut(s) 48, 867, 2146, 2952
AlwI GGATC 4 cut(s) 107, 785, 1628, 2789
AlwNI CAGNNNCTG 1 cut(s) 658
Ama87I CYCGRG 1 cut(s) 2693
AoxI GGCC 6 cut(s) 88, 952, 1938, 2275, 2517, 3017
ApaI GGGCCC 1 cut(s) 2521
ApeKI GCWGC 9 cut(s) 335, 341, 363, 944, 1154, 1327, 1369, 1421, 2197
ApoI RAATTY 7 cut(s) 437, 935, 1349, 2174, 2290, 2338, 2452
Asp700I GAANNNNTTC 1 cut(s) 687
AspS9I GGNCC 5 cut(s) 486, 917, 953, 2517, 2518
AsuC2I CCSGG 1 cut(s) 1667
AsuHPI GGTGA 6 cut(s) 85, 582, 699, 707, 1780, 2470
AsuII TTCGAA 1 cut(s) 531
AvaI CYCGRG 1 cut(s) 2693
AvaII GGWCC 2 cut(s) 486, 917
AxyI CCTNAGG 2 cut(s) 381, 1374
BaeGI GKGCMC 2 cut(s) 2521, 2611
BaeI ACNNNNGTAYC 4 cut(s) 1716, 1749, 1941, 1974
BalI TGGCCA 1 cut(s) 1940
BanI GGYRCC 2 cut(s) 557, 1442
BanII GRGCYC 2 cut(s) 396, 2521
BarI GAAGNNNNNNTAC 4 cut(s) 1763, 1795, 2340, 2372
BbsI GAAGAC 5 cut(s) 183, 225, 1031, 2695, 3048
Bbv12I GWGCWC 1 cut(s) 414
BbvCI CCTCAGC 1 cut(s) 168
BbvI GCAGC 9 cut(s) 347, 353, 375, 956, 1141, 1314, 1381, 1433, 2184
BccI CCATC 4 cut(s) 1166, 1618, 1706, 2806
BceAI ACGGC 2 cut(s) 103, 3032
BciT130I CCWGG 3 cut(s) 596, 653, 711
BciVI GTATCC 1 cut(s) 1327
BcnI CCSGG 1 cut(s) 1667
BcoDI GTCTC 4 cut(s) 48, 867, 2146, 2952
BfaI CTAG 9 cut(s) 104, 407, 842, 986, 1131, 1932, 2735, 2966, 3081
BfmI CTRYAG 3 cut(s) 633, 1328, 1725
BfuI GTATCC 1 cut(s) 1327
BglII AGATCT 2 cut(s) 1458, 2697
Bme1390I CCNGG 4 cut(s) 596, 653, 711, 1667
Bme18I GGWCC 2 cut(s) 486, 917
BmeT110I CYCGRG 1 cut(s) 2693
BmgT120I GGNCC 5 cut(s) 486, 917, 953, 2517, 2518
BmiI GGNNCC 7 cut(s) 487, 559, 918, 1444, 2519, 2553, 3052
BmrFI CCNGG 4 cut(s) 596, 653, 711, 1667
BmrI ACTGGG 1 cut(s) 1347
BmsI GCATC 8 cut(s) 658, 751, 1167, 1189, 1669, 1839, 2413, 2975
BmuI ACTGGG 1 cut(s) 1347
BpiI GAAGAC 5 cut(s) 183, 225, 1031, 2695, 3048
BpmI CTGGAG 2 cut(s) 1623, 2000
Bpu10I CCTNAGC 2 cut(s) 168, 2570
Bpu14I TTCGAA 1 cut(s) 531
BpuEI CTTGAG 4 cut(s) 536, 896, 906, 2170
BpuMI CCSGG 1 cut(s) 1667
Bsa29I ATCGAT 1 cut(s) 2821
BsaBI GATNNNNATC 1 cut(s) 1218
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 9 cut(s) 141, 594, 612, 765, 823, 1087, 1571, 1808, 3046
BsaXI ACNNNNNCTCC 2 cut(s) 2708, 2738
Bsc4I CCNNNNNNNGG 7 cut(s) 122, 382, 618, 1577, 2514, 2847, 2958
Bse1I ACTGG 5 cut(s) 1342, 1561, 1758, 2427, 2924
Bse21I CCTNAGG 2 cut(s) 381, 1374
Bse3DI GCAATG 3 cut(s) 98, 351, 1655
Bse8I GATNNNNATC 1 cut(s) 1218
BseBI CCWGG 3 cut(s) 596, 653, 711
BseCI ATCGAT 1 cut(s) 2821
BseDI CCNNGG 9 cut(s) 141, 594, 612, 765, 823, 1087, 1571, 1808, 3046
BseJI GATNNNNATC 1 cut(s) 1218
BseLI CCNNNNNNNGG 7 cut(s) 122, 382, 618, 1577, 2514, 2847, 2958
BseMI GCAATG 3 cut(s) 98, 351, 1655
BseNI ACTGG 5 cut(s) 1342, 1561, 1758, 2427, 2924
BseRI GAGGAG 2 cut(s) 278, 1379
BseSI GKGCMC 2 cut(s) 2521, 2611
BseX3I CGGCCG 1 cut(s) 3017
BseXI GCAGC 9 cut(s) 347, 353, 375, 956, 1141, 1314, 1381, 1433, 2184
BseYI CCCAGC 3 cut(s) 2247, 2259, 2765
Bsh1236I CGCG 1 cut(s) 2614
Bsh1285I CGRYCG 1 cut(s) 3020
BshFI GGCC 6 cut(s) 90, 954, 1940, 2277, 2519, 3019
BshNI GGYRCC 2 cut(s) 557, 1442
BshVI ATCGAT 1 cut(s) 2821
BsiEI CGRYCG 1 cut(s) 3020
BsiHKAI GWGCWC 1 cut(s) 414
BsiHKCI CYCGRG 1 cut(s) 2693
BsiSI CCGG 3 cut(s) 10, 1667, 3020
BslFI GGGAC 2 cut(s) 1285, 2488
BslI CCNNNNNNNGG 7 cut(s) 122, 382, 618, 1577, 2514, 2847, 2958
BsmAI GTCTC 4 cut(s) 48, 867, 2146, 2952
BsmFI GGGAC 2 cut(s) 1285, 2488
BsmI GAATGC 1 cut(s) 1114
BsnI GGCC 6 cut(s) 90, 954, 1940, 2277, 2519, 3019
BsoBI CYCGRG 1 cut(s) 2693
Bsp119I TTCGAA 1 cut(s) 531
Bsp120I GGGCCC 1 cut(s) 2517
Bsp1286I GDGCHC 4 cut(s) 396, 414, 2521, 2611
Bsp1407I TGTACA 2 cut(s) 807, 2347
Bsp143I GATC 8 cut(s) 112, 777, 1458, 1633, 2068, 2527, 2697, 2794
Bsp19I CCATGG 3 cut(s) 612, 765, 823
BspANI GGCC 6 cut(s) 90, 954, 1940, 2277, 2519, 3019
BspDI ATCGAT 1 cut(s) 2821
BspFNI CGCG 1 cut(s) 2614
BspLI GGNNCC 7 cut(s) 487, 559, 918, 1444, 2519, 2553, 3052
BspMAI CTGCAG 1 cut(s) 1332
BspPI GGATC 4 cut(s) 107, 785, 1628, 2789
BspT104I TTCGAA 1 cut(s) 531
BspT107I GGYRCC 2 cut(s) 557, 1442
BsrBI CCGCTC 2 cut(s) 619, 2485
BsrDI GCAATG 3 cut(s) 98, 351, 1655
BsrGI TGTACA 2 cut(s) 807, 2347
BsrI ACTGG 5 cut(s) 1342, 1561, 1758, 2427, 2924
BssECI CCNNGG 9 cut(s) 141, 594, 612, 765, 823, 1087, 1571, 1808, 3046
BssMI GATC 8 cut(s) 112, 777, 1458, 1633, 2068, 2527, 2697, 2794
BssNAI GTATAC 1 cut(s) 1751
BssNI GRCGYC 1 cut(s) 30
BssT1I CCWWGG 7 cut(s) 141, 612, 765, 823, 1087, 1571, 3046
Bst1107I GTATAC 1 cut(s) 1751
Bst2UI CCWGG 3 cut(s) 596, 653, 711
Bst4CI ACNGT 3 cut(s) 68, 1997, 2599
Bst6I CTCTTC 4 cut(s) 183, 255, 1856, 2146
BstACI GRCGYC 1 cut(s) 30
BstAUI TGTACA 2 cut(s) 807, 2347
BstBI TTCGAA 1 cut(s) 531
BstC8I GCNNGC 3 cut(s) 7, 396, 673
BstDSI CCRYGG 4 cut(s) 612, 765, 823, 1808
BstEII GGTNACC 1 cut(s) 713
BstFNI CGCG 1 cut(s) 2614
BstKTI GATC 8 cut(s) 115, 780, 1461, 1636, 2071, 2530, 2700, 2797
BstMAI GTCTC 4 cut(s) 48, 867, 2146, 2952
BstMBI GATC 8 cut(s) 112, 777, 1458, 1633, 2068, 2527, 2697, 2794
BstMCI CGRYCG 1 cut(s) 3020
BstMWI GCNNNNNNNGC 2 cut(s) 341, 1937
BstNI CCWGG 3 cut(s) 596, 653, 711
BstNSI RCATGY 3 cut(s) 418, 1433, 2204
BstPI GGTNACC 1 cut(s) 713
BstSCI CCNGG 4 cut(s) 594, 651, 709, 1665
BstSFI CTRYAG 3 cut(s) 633, 1328, 1725
BstSLI GKGCMC 2 cut(s) 2521, 2611
BstUI CGCG 1 cut(s) 2614
BstV1I GCAGC 9 cut(s) 347, 353, 375, 956, 1141, 1314, 1381, 1433, 2184
BstV2I GAAGAC 5 cut(s) 183, 225, 1031, 2695, 3048
BstX2I RGATCY 4 cut(s) 1458, 1633, 2697, 2794
BstXI CCANNNNNNTGG 1 cut(s) 1311
BstYI RGATCY 4 cut(s) 1458, 1633, 2697, 2794
BstZ17I GTATAC 1 cut(s) 1751
BstZI CGGCCG 1 cut(s) 3017
Bsu15I ATCGAT 1 cut(s) 2821
Bsu36I CCTNAGG 2 cut(s) 381, 1374
BsuI GTATCC 1 cut(s) 1327
BsuRI GGCC 6 cut(s) 90, 954, 1940, 2277, 2519, 3019
BsuTUI ATCGAT 1 cut(s) 2821
BtgI CCRYGG 4 cut(s) 612, 765, 823, 1808
BtsIMutI CAGTG 4 cut(s) 1224, 1568, 2434, 2609
Cac8I GCNNGC 3 cut(s) 7, 396, 673
CaiI CAGNNNCTG 1 cut(s) 658
Cfr13I GGNCC 5 cut(s) 486, 917, 953, 2517, 2518
ClaI ATCGAT 1 cut(s) 2821
CsiI ACCWGGT 2 cut(s) 651, 709
Csp6I GTAC 9 cut(s) 626, 808, 872, 1780, 1794, 2348, 2665, 2882, 2923
CviQI GTAC 9 cut(s) 626, 808, 872, 1780, 1794, 2348, 2665, 2882, 2923
DpnI GATC 8 cut(s) 114, 779, 1460, 1635, 2070, 2529, 2699, 2796
DpnII GATC 8 cut(s) 112, 777, 1458, 1633, 2068, 2527, 2697, 2794
DraI TTTAAA 1 cut(s) 1801
EaeI YGGCCR 2 cut(s) 1938, 3017
EagI CGGCCG 1 cut(s) 3017
Eam1104I CTCTTC 4 cut(s) 183, 255, 1856, 2146
EarI CTCTTC 4 cut(s) 183, 255, 1856, 2146
EclXI CGGCCG 1 cut(s) 3017
Eco130I CCWWGG 7 cut(s) 141, 612, 765, 823, 1087, 1571, 3046
Eco147I AGGCCT 1 cut(s) 2277
Eco24I GRGCYC 2 cut(s) 396, 2521
Eco47I GGWCC 2 cut(s) 486, 917
Eco52I CGGCCG 1 cut(s) 3017
Eco57I CTGAAG 6 cut(s) 180, 311, 1488, 1614, 2006, 3030
Eco81I CCTNAGG 2 cut(s) 381, 1374
Eco88I CYCGRG 1 cut(s) 2693
Eco91I GGTNACC 1 cut(s) 713
EcoO109I RGGNCCY 4 cut(s) 486, 917, 2517, 2518
EcoO65I GGTNACC 1 cut(s) 713
EcoRI GAATTC 1 cut(s) 935
EcoRII CCWGG 3 cut(s) 594, 651, 709
EcoT14I CCWWGG 7 cut(s) 141, 612, 765, 823, 1087, 1571, 3046
EcoT22I ATGCAT 1 cut(s) 1182
EcoT38I GRGCYC 2 cut(s) 396, 2521
ErhI CCWWGG 7 cut(s) 141, 612, 765, 823, 1087, 1571, 3046
FalI AAGNNNNNCTT 2 cut(s) 545, 577
FaqI GGGAC 2 cut(s) 1285, 2488
FauI CCCGC 4 cut(s) 612, 780, 2478, 2619
FblI GTMKAC 3 cut(s) 1750, 1992, 2395
FriOI GRGCYC 2 cut(s) 396, 2521
FspBI CTAG 9 cut(s) 104, 407, 842, 986, 1131, 1932, 2735, 2966, 3081
GsaI CCCAGC 3 cut(s) 2251, 2263, 2769
GsuI CTGGAG 2 cut(s) 1623, 2000
HaeIII GGCC 6 cut(s) 90, 954, 1940, 2277, 2519, 3019
HapII CCGG 3 cut(s) 10, 1667, 3020
Hin1I GRCGYC 1 cut(s) 30
HindIII AAGCTT 1 cut(s) 2009
HpaII CCGG 3 cut(s) 10, 1667, 3020
HphI GGTGA 6 cut(s) 85, 582, 699, 707, 1780, 2470
Hpy166II GTNNAC 5 cut(s) 109, 979, 1751, 1993, 2396
Hpy8I GTNNAC 5 cut(s) 109, 979, 1751, 1993, 2396
Hpy99I CGWCG 2 cut(s) 32, 35
HpyAV CCTTC 7 cut(s) 325, 944, 1981, 2267, 2412, 2813, 2896
HpyCH4III ACNGT 3 cut(s) 68, 1997, 2599
HpyCH4IV ACGT 4 cut(s) 30, 906, 2628, 2649
HpyF10VI GCNNNNNNNGC 2 cut(s) 341, 1937
HpySE526I ACGT 4 cut(s) 30, 906, 2628, 2649
Hsp92I GRCGYC 1 cut(s) 30
Kzo9I GATC 8 cut(s) 112, 777, 1458, 1633, 2068, 2527, 2697, 2794
LmnI GCTCC 7 cut(s) 616, 1324, 1366, 1981, 2098, 2581, 3050
Lsp1109I GCAGC 9 cut(s) 347, 353, 375, 956, 1141, 1314, 1381, 1433, 2184
LweI GCATC 8 cut(s) 658, 751, 1167, 1189, 1669, 1839, 2413, 2975
MabI ACCWGGT 2 cut(s) 651, 709
MaeI CTAG 9 cut(s) 104, 407, 842, 986, 1131, 1932, 2735, 2966, 3081
MaeII ACGT 4 cut(s) 30, 906, 2628, 2649
MaeIII GTNAC 5 cut(s) 464, 713, 902, 2135, 2617
MalI GATC 8 cut(s) 114, 779, 1460, 1635, 2070, 2529, 2699, 2796
MbiI CCGCTC 2 cut(s) 619, 2485
MboI GATC 8 cut(s) 112, 777, 1458, 1633, 2068, 2527, 2697, 2794
MfeI CAATTG 2 cut(s) 2942, 3085
MflI RGATCY 4 cut(s) 1458, 1633, 2697, 2794
MhlI GDGCHC 4 cut(s) 396, 414, 2521, 2611
MlsI TGGCCA 1 cut(s) 1940
MluNI TGGCCA 1 cut(s) 1940
MlyI GAGTC 9 cut(s) 54, 140, 869, 1223, 2143, 2510, 2685, 2738, 3032
MmeI TCCRAC 2 cut(s) 1144, 2939
Mox20I TGGCCA 1 cut(s) 1940
Mph1103I ATGCAT 1 cut(s) 1182
MroXI GAANNNNTTC 1 cut(s) 687
MscI TGGCCA 1 cut(s) 1940
MslI CAYNNNNRTG 4 cut(s) 1033, 1309, 2120, 2996
Msp20I TGGCCA 1 cut(s) 1940
MspA1I CMGCKG 1 cut(s) 947
MspI CCGG 3 cut(s) 10, 1667, 3020
MspR9I CCNGG 4 cut(s) 596, 653, 711, 1667
MunI CAATTG 2 cut(s) 2942, 3085
Mva1269I GAATGC 1 cut(s) 1114
MvaI CCWGG 3 cut(s) 596, 653, 711
MvnI CGCG 1 cut(s) 2614
MwoI GCNNNNNNNGC 2 cut(s) 341, 1937
NciI CCSGG 1 cut(s) 1667
NcoI CCATGG 3 cut(s) 612, 765, 823
NdeII GATC 8 cut(s) 112, 777, 1458, 1633, 2068, 2527, 2697, 2794
NlaIV GGNNCC 7 cut(s) 487, 559, 918, 1444, 2519, 2553, 3052
NmeAIII GCCGAG 1 cut(s) 1767
NmuCI GTSAC 2 cut(s) 713, 2135
NsiI ATGCAT 1 cut(s) 1182
NspI RCATGY 3 cut(s) 418, 1433, 2204
NspV TTCGAA 1 cut(s) 531
PaeR7I CTCGAG 1 cut(s) 2693
PceI AGGCCT 1 cut(s) 2277
PcsI WCGNNNNNNNCGW 1 cut(s) 64
PctI GAATGC 1 cut(s) 1114
PdmI GAANNNNTTC 1 cut(s) 687
PfeI GAWTC 5 cut(s) 317, 638, 729, 1472, 2032
PflFI GACNNNGTC 1 cut(s) 29
PfoI TCCNGGA 1 cut(s) 1665
PleI GAGTC 9 cut(s) 54, 140, 868, 1223, 2142, 2509, 2685, 2738, 3031
PpsI GAGTC 9 cut(s) 54, 140, 868, 1223, 2142, 2509, 2685, 2738, 3031
PpuMI RGGWCCY 2 cut(s) 486, 917
PsiI TTATAA 1 cut(s) 2705
Psp5II RGGWCCY 2 cut(s) 486, 917
Psp6I CCWGG 3 cut(s) 594, 651, 709
PspEI GGTNACC 1 cut(s) 713
PspFI CCCAGC 3 cut(s) 2247, 2259, 2765
PspGI CCWGG 3 cut(s) 594, 651, 709
PspN4I GGNNCC 7 cut(s) 487, 559, 918, 1444, 2519, 2553, 3052
PspOMI GGGCCC 1 cut(s) 2517
PspPI GGNCC 5 cut(s) 486, 917, 953, 2517, 2518
PspPPI RGGWCCY 2 cut(s) 486, 917
PstI CTGCAG 1 cut(s) 1332
PstNI CAGNNNCTG 1 cut(s) 658
PsuI RGATCY 4 cut(s) 1458, 1633, 2697, 2794
PsyI GACNNNGTC 1 cut(s) 29
RsaI GTAC 9 cut(s) 627, 809, 873, 1781, 1795, 2349, 2666, 2883, 2924
RsaNI GTAC 9 cut(s) 626, 808, 872, 1780, 1794, 2348, 2665, 2882, 2923
RseI CAYNNNNRTG 4 cut(s) 1033, 1309, 2120, 2996
Sau3AI GATC 8 cut(s) 112, 777, 1458, 1633, 2068, 2527, 2697, 2794
Sau96I GGNCC 5 cut(s) 486, 917, 953, 2517, 2518
SchI GAGTC 9 cut(s) 54, 140, 869, 1223, 2143, 2510, 2685, 2738, 3032
ScrFI CCNGG 4 cut(s) 596, 653, 711, 1667
SduI GDGCHC 4 cut(s) 396, 414, 2521, 2611
SexAI ACCWGGT 2 cut(s) 651, 709
SfaNI GCATC 8 cut(s) 658, 751, 1167, 1189, 1669, 1839, 2413, 2975
SfcI CTRYAG 3 cut(s) 633, 1328, 1725
Sfr274I CTCGAG 1 cut(s) 2693
SfuI TTCGAA 1 cut(s) 531
SinI GGWCC 2 cut(s) 486, 917
SlaI CTCGAG 1 cut(s) 2693
SmiMI CAYNNNNRTG 4 cut(s) 1033, 1309, 2120, 2996
SmlI CTYRAG 5 cut(s) 551, 875, 921, 2149, 2693
SmoI CTYRAG 5 cut(s) 551, 875, 921, 2149, 2693
SseBI AGGCCT 1 cut(s) 2277
SspI AATATT 1 cut(s) 798
SspMI CTAG 9 cut(s) 104, 407, 842, 986, 1131, 1932, 2735, 2966, 3081
StuI AGGCCT 1 cut(s) 2277
StyD4I CCNGG 4 cut(s) 594, 651, 709, 1665
StyI CCWWGG 7 cut(s) 141, 612, 765, 823, 1087, 1571, 3046
TaaI ACNGT 3 cut(s) 68, 1997, 2599
TaiI ACGT 4 cut(s) 33, 909, 2631, 2652
TaqI TCGA 4 cut(s) 81, 531, 2694, 2821
TatI WGTACW 6 cut(s) 807, 871, 1793, 2347, 2664, 2881
TauI GCSGC 2 cut(s) 341, 678
TfiI GAWTC 5 cut(s) 317, 638, 729, 1472, 2032
TscAI CASTG 4 cut(s) 1231, 1568, 2434, 2609
TseFI GTSAC 2 cut(s) 713, 2135
TseI GCWGC 9 cut(s) 335, 341, 363, 944, 1154, 1327, 1369, 1421, 2197
Tsp45I GTSAC 2 cut(s) 713, 2135
TspGWI ACGGA 1 cut(s) 495
TspRI CASTG 4 cut(s) 1231, 1568, 2434, 2609
Tth111I GACNNNGTC 1 cut(s) 29
VpaK11BI GGWCC 2 cut(s) 486, 917
XapI RAATTY 7 cut(s) 437, 935, 1349, 2174, 2290, 2338, 2452
XbaI TCTAGA 1 cut(s) 406
XceI RCATGY 3 cut(s) 418, 1433, 2204
XhoI CTCGAG 1 cut(s) 2693
XmiI GTMKAC 3 cut(s) 1750, 1992, 2395
XmnI GAANNNNTTC 1 cut(s) 687
XspI CTAG 9 cut(s) 104, 407, 842, 986, 1131, 1932, 2735, 2966, 3081
ZraI GACGTC 1 cut(s) 31
Zsp2I ATGCAT 1 cut(s) 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.