Rh5AG477400

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000183
Physical Location & Seq
Reverse (-)
82086183 .. 82094168
7986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG477400.1

Sequence Viewer

Length: 1764 bp
ATGGGTCGCCCCACTTTCTCCTCCGATGAAGTTGTGATCAAGTCTCCGAATGATAAACGACTCTACAGATTGATTAAGCTTGAGAATGGCCTCACTGCATTGCTCATTCATGATCCTGATATATACCCACAAGGGTTTAAGAGCCTTGAGCCCTGTGAAGATGAAGAAGCAGCCAAAGGTGGAGATGGTGAGGTTGGCAATAAGGGAAAGGGAGGTGCTTTACGGACTAAGAAGGCAGCAGCAGCAATGTGCGTTGGAATAGGCAGCTGGTCTGACCCTATGGAGGCACAGGGGCTTGCACACTTTCTAGAACACATGCTGTTCATGGGGAGTACAAAATTCCCAGTTGAAAATGAGTATGATAGTTACTTGTCCAAGCATGGAGGGTCATCAAATGCATACACCTGTGCAGAGCATACTTGCTACTTTTTTGAAGTGAAACCGGAGTTTCTTAAGGGTGCCTTGACAAGATTTTCTCAGTTCTTTGTTTCACCTCTGATAAAAAGTGAATCCATGGAGCGAGAGATACAGGCTATAGATTCAGAGTTTAACAGGGTTCTGCAAAATGACTTTTGCCGCCTTCGACAACTTCAATGCTATACATCCACACCAGGTCACCCGTTTAATAGATTTTCTCGAGGAAATAAGAAGAGCTTGGATGATGCAACGAAAAAGGGGATCAACTTGCAGGAACAAATACTGAAATTGTACAGAAACTATTACCGCGGTGGATTAATGAAGCTAGTTGTCATTGGTGGAGAGTTTCAGTGCGAGCCTTGGTTCGGGTCACATTATACCGAGGAAGATATATCTCCAGCTTTGATGGAATTGTGGAAGGATCCTCCAGAAATTGATGTATCGTTGCATCTGCGGGCAAAAAATGAGTTCATTCCTAGTGACTTTTCCATCCGTTCTGATGGGATGTGTCTTGATATTTCAAATATATCTTCTCCGGCATGTATGTTTGATGAACCATTGATGAAGATCTGGTACAAGCTTGATAGTACTTTTAAACTTCCACGGGCAAATGCGTACTTCTTCATCAGAATGAAAGGTGGATATGATAATGTAAAAAGTAGTGTTTTGACAGACTTATACATCGACCTTCTTCAAGATGAGCTCAGTGAGATCATCTATCAGGCTTATGTTGCCTGCTTGGGGACTTCTATTTCTGTGTTTACCGACAAACTTCAGCTGCAGGTGTATGGTTTCAATGATAAGCTTCCAGCTCTGTTGTCAAAAATTGTGGAAACAGTAAAGAGCTTCTTGCCAACTGATGATCGTTTTGAGGACAAAGGAGCAGCTCGGTCATCTGAGTACAACCCAATCCACCTACAAGGAAGACTAGACAACTTCATAAATGGTCTGGAAGATTTGTTGGAAGGACTGGATGATGATTCTTTTGAGAATTATAAAGCTGGACTAATGGAAAATCTTTTGAAGAAAGATACATCCCTCACGTGTGAAACCAATCGATTGTGGAATGAGATTTATATTAAAAGGTATATGTTTGACTACTCAAAAAAGGCAGCAGAAGAACTCAGATGCATTCAGAAGGGGGATGTTATCAACTTTTACAAGACATATTTGCAACAATCATCTCCCAAGTGTCGAAGACTTGCAACTCGTGTTTGGGGTTGCAACACCGACTTCAAAGAGGCAGAAGTAGCACAACTGGAGTCTGTGCAAGTCATTGAAGACGTTGCAGCCTTTAAGATGTCATCGAAGTTCTATAAACCTAAGATAAATACTCTGGATACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

587

Amino Acids

66.91

Weight (kDa)

5.48

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 76 - 189 5.1e-31 Insulinase (Peptidase family M16)
Peptidase_M16_M PF16187 256 - 431 3.8e-51 Middle or third domain of peptidase_M16
PqqF-like_C_4 PF22456 436 - 494 2.7e-07 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1413
AarI CACCTGC 1 cut(s) 1189
Acc36I ACCTGC 1 cut(s) 1189
AccB1I GGYRCC 1 cut(s) 458
AccII CGCG 1 cut(s) 726
AciI CCGC 4 cut(s) 577, 724, 726, 871
AclWI GGATC 4 cut(s) 107, 686, 833, 846
AcsI RAATTY 1 cut(s) 338
AcuI CTGAAG 1 cut(s) 1175
AcvI CACGTG 1 cut(s) 1461
AfaI GTAC 6 cut(s) 334, 710, 992, 1006, 1034, 1319
AfiI CCNNNNNNNGG 3 cut(s) 283, 782, 1158
AflII CTTAAG 1 cut(s) 452
AflIII ACRYGT 1 cut(s) 1460
AgsI TTSAA 9 cut(s) 350, 434, 593, 939, 1112, 1213, 1441, 1654, 1697
AjnI CCWGG 1 cut(s) 610
Alw21I GWGCWC 1 cut(s) 1122
Alw26I GTCTC 1 cut(s) 48
AlwI GGATC 4 cut(s) 107, 686, 833, 846
Ama87I CYCGRG 1 cut(s) 636
AoxI GGCC 1 cut(s) 88
ApeKI GCWGC 9 cut(s) 170, 236, 239, 242, 264, 1195, 1301, 1529, 1706
ApoI RAATTY 1 cut(s) 338
AseI ATTAAT 1 cut(s) 734
AsuHPI GGTGA 3 cut(s) 200, 483, 608
AvaI CYCGRG 1 cut(s) 636
BamHI GGATCC 1 cut(s) 838
BanI GGYRCC 1 cut(s) 458
BanII GRGCYC 2 cut(s) 153, 1122
BarI GAAGNNNNNNTAC 2 cut(s) 974, 1006
BauI CACGAG 1 cut(s) 1626
BbrPI CACGTG 1 cut(s) 1461
BbsI GAAGAC 3 cut(s) 1348, 1621, 1704
Bbv12I GWGCWC 1 cut(s) 1122
BbvI GCAGC 9 cut(s) 182, 248, 251, 254, 276, 1182, 1313, 1541, 1718
BccI CCATC 4 cut(s) 179, 817, 911, 914
BciT130I CCWGG 1 cut(s) 612
BciVI GTATCC 1 cut(s) 1750
BclI TGATCA 1 cut(s) 36
BcoDI GTCTC 1 cut(s) 48
BfaI CTAG 4 cut(s) 308, 743, 894, 1346
BfmI CTRYAG 3 cut(s) 64, 534, 1196
BfrI CTTAAG 1 cut(s) 452
BfuAI ACCTGC 1 cut(s) 1189
BfuI GTATCC 1 cut(s) 1750
BglII AGATCT 1 cut(s) 984
BmcAI AGTACT 1 cut(s) 1006
Bme1390I CCNGG 1 cut(s) 612
BmeT110I CYCGRG 1 cut(s) 636
BmiI GGNNCC 2 cut(s) 460, 840
BmrFI CCNGG 1 cut(s) 612
BmrI ACTGGG 1 cut(s) 338
BmsI GCATC 3 cut(s) 652, 874, 1535
BmuI ACTGGG 1 cut(s) 338
BpiI GAAGAC 3 cut(s) 1348, 1621, 1704
BpmI CTGGAG 3 cut(s) 798, 828, 1697
BpuEI CTTGAG 2 cut(s) 101, 167
Bsa29I ATCGAT 1 cut(s) 1474
BsaAI YACGTR 1 cut(s) 1461
BsaBI GATNNNNATC 1 cut(s) 983
BsaJI CCNNGG 5 cut(s) 513, 724, 776, 798, 1019
BsaWI WCCGGW 1 cut(s) 442
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 782, 1158
Bse1I ACTGG 3 cut(s) 344, 1392, 1680
Bse3DI GCAATG 2 cut(s) 98, 252
Bse8I GATNNNNATC 1 cut(s) 983
BseBI CCWGG 1 cut(s) 612
BseCI ATCGAT 1 cut(s) 1474
BseDI CCNNGG 5 cut(s) 513, 724, 776, 798, 1019
BseGI GGATG 7 cut(s) 602, 664, 906, 927, 1396, 1451, 1567
BseJI GATNNNNATC 1 cut(s) 983
BseLI CCNNNNNNNGG 3 cut(s) 283, 782, 1158
BseMI GCAATG 2 cut(s) 98, 252
BseMII CTCAG 4 cut(s) 491, 1135, 1305, 1555
BseNI ACTGG 3 cut(s) 344, 1392, 1680
BseRI GAGGAG 1 cut(s) 10
BseXI GCAGC 9 cut(s) 182, 248, 251, 254, 276, 1182, 1313, 1541, 1718
BsgI GTGCAG 1 cut(s) 429
Bsh1236I CGCG 1 cut(s) 726
BshFI GGCC 1 cut(s) 90
BshNI GGYRCC 1 cut(s) 458
BshVI ATCGAT 1 cut(s) 1474
BsiHKAI GWGCWC 1 cut(s) 1122
BsiHKCI CYCGRG 1 cut(s) 636
BsiSI CCGG 2 cut(s) 443, 953
BslFI GGGAC 1 cut(s) 1174
BslI CCNNNNNNNGG 3 cut(s) 283, 782, 1158
BsmAI GTCTC 1 cut(s) 48
BsmFI GGGAC 1 cut(s) 1174
BsmI GAATGC 1 cut(s) 1548
BsnI GGCC 1 cut(s) 90
BsoBI CYCGRG 1 cut(s) 636
Bsp1286I GDGCHC 2 cut(s) 153, 1122
Bsp1407I TGTACA 1 cut(s) 708
Bsp143I GATC 7 cut(s) 36, 112, 678, 838, 984, 1128, 1279
Bsp19I CCATGG 1 cut(s) 513
BspACI CCGC 4 cut(s) 577, 724, 726, 871
BspANI GGCC 1 cut(s) 90
BspCNI CTCAG 4 cut(s) 490, 1134, 1306, 1554
BspDI ATCGAT 1 cut(s) 1474
BspFNI CGCG 1 cut(s) 726
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 2 cut(s) 460, 840
BspMAI CTGCAG 1 cut(s) 1200
BspMI ACCTGC 1 cut(s) 1189
BspPI GGATC 4 cut(s) 107, 686, 833, 846
BspQI GCTCTTC 1 cut(s) 644
BspT107I GGYRCC 1 cut(s) 458
BspTI CTTAAG 1 cut(s) 452
BsrDI GCAATG 2 cut(s) 98, 252
BsrGI TGTACA 1 cut(s) 708
BsrI ACTGG 3 cut(s) 344, 1392, 1680
BssECI CCNNGG 5 cut(s) 513, 724, 776, 798, 1019
BssMI GATC 7 cut(s) 36, 112, 678, 838, 984, 1128, 1279
BssSI CACGAG 1 cut(s) 1626
BssT1I CCWWGG 2 cut(s) 513, 776
Bst2BI CACGAG 1 cut(s) 1626
Bst2UI CCWGG 1 cut(s) 612
Bst4CI ACNGT 1 cut(s) 1255
Bst6I CTCTTC 1 cut(s) 644
BstAFI CTTAAG 1 cut(s) 452
BstAUI TGTACA 1 cut(s) 708
BstBAI YACGTR 1 cut(s) 1461
BstC8I GCNNGC 4 cut(s) 297, 773, 873, 1153
BstDEI CTNAG 6 cut(s) 228, 477, 1121, 1314, 1541, 1740
BstDSI CCRYGG 3 cut(s) 513, 724, 1019
BstEII GGTNACC 1 cut(s) 614
BstF5I GGATG 7 cut(s) 602, 664, 906, 927, 1396, 1451, 1567
BstFNI CGCG 1 cut(s) 726
BstKTI GATC 7 cut(s) 39, 115, 681, 841, 987, 1131, 1282
BstMAI GTCTC 1 cut(s) 48
BstMBI GATC 7 cut(s) 36, 112, 678, 838, 984, 1128, 1279
BstMWI GCNNNNNNNGC 3 cut(s) 242, 1148, 1667
BstNI CCWGG 1 cut(s) 612
BstNSI RCATGY 2 cut(s) 319, 960
BstPI GGTNACC 1 cut(s) 614
BstSCI CCNGG 1 cut(s) 610
BstSFI CTRYAG 3 cut(s) 64, 534, 1196
BstUI CGCG 1 cut(s) 726
BstV1I GCAGC 9 cut(s) 182, 248, 251, 254, 276, 1182, 1313, 1541, 1718
BstV2I GAAGAC 3 cut(s) 1348, 1621, 1704
BstX2I RGATCY 2 cut(s) 838, 984
BstYI RGATCY 2 cut(s) 838, 984
Bsu15I ATCGAT 1 cut(s) 1474
BsuI GTATCC 1 cut(s) 1750
BsuRI GGCC 1 cut(s) 90
BsuTUI ATCGAT 1 cut(s) 1474
BtgI CCRYGG 3 cut(s) 513, 724, 1019
BtsCI GGATG 7 cut(s) 602, 664, 906, 927, 1396, 1451, 1567
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 3 cut(s) 93, 773, 1129
BveI ACCTGC 1 cut(s) 1189
Cac8I GCNNGC 4 cut(s) 297, 773, 873, 1153
CciI TCATGA 1 cut(s) 109
Cfr42I CCGCGG 1 cut(s) 727
ClaI ATCGAT 1 cut(s) 1474
CsiI ACCWGGT 1 cut(s) 610
Csp6I GTAC 6 cut(s) 333, 709, 991, 1005, 1033, 1318
CspCI CAANNNNNGTGG 2 cut(s) 1227, 1262
CviAII CATG 6 cut(s) 110, 316, 325, 380, 514, 957
CviQI GTAC 6 cut(s) 333, 709, 991, 1005, 1033, 1318
DdeI CTNAG 6 cut(s) 228, 477, 1121, 1314, 1541, 1740
DpnI GATC 7 cut(s) 38, 114, 680, 840, 986, 1130, 1281
DpnII GATC 7 cut(s) 36, 112, 678, 838, 984, 1128, 1279
DraI TTTAAA 1 cut(s) 1012
Eam1104I CTCTTC 1 cut(s) 644
EarI CTCTTC 1 cut(s) 644
Ecl136II GAGCTC 1 cut(s) 1120
Eco130I CCWWGG 2 cut(s) 513, 776
Eco24I GRGCYC 2 cut(s) 153, 1122
Eco53kI GAGCTC 1 cut(s) 1120
Eco57I CTGAAG 1 cut(s) 1175
Eco72I CACGTG 1 cut(s) 1461
Eco88I CYCGRG 1 cut(s) 636
Eco91I GGTNACC 1 cut(s) 614
EcoICRI GAGCTC 1 cut(s) 1120
EcoO65I GGTNACC 1 cut(s) 614
EcoRII CCWGG 1 cut(s) 610
EcoT14I CCWWGG 2 cut(s) 513, 776
EcoT22I ATGCAT 2 cut(s) 400, 1550
EcoT38I GRGCYC 2 cut(s) 153, 1122
ErhI CCWWGG 2 cut(s) 513, 776
FaeI CATG 6 cut(s) 113, 319, 328, 383, 517, 960
FalI AAGNNNNNCTT 6 cut(s) 446, 478, 638, 670, 1250, 1282
FaqI GGGAC 1 cut(s) 1174
FatI CATG 6 cut(s) 109, 315, 324, 379, 513, 956
FauI CCCGC 1 cut(s) 864
FbaI TGATCA 1 cut(s) 36
FokI GGATG 7 cut(s) 589, 671, 893, 934, 1403, 1438, 1574
FriOI GRGCYC 2 cut(s) 153, 1122
FspBI CTAG 4 cut(s) 308, 743, 894, 1346
GsuI CTGGAG 3 cut(s) 798, 828, 1697
HaeIII GGCC 1 cut(s) 90
HapII CCGG 2 cut(s) 443, 953
Hin1II CATG 6 cut(s) 113, 319, 328, 383, 517, 960
HindIII AAGCTT 3 cut(s) 77, 995, 1220
HinfI GANTC 5 cut(s) 60, 509, 539, 1397, 1679
HpaII CCGG 2 cut(s) 443, 953
HphI GGTGA 3 cut(s) 200, 483, 608
Hpy166II GTNNAC 1 cut(s) 1179
Hpy188III TCNNGA 9 cut(s) 110, 116, 308, 636, 845, 929, 1112, 1367, 1754
Hpy8I GTNNAC 1 cut(s) 1179
HpyAV CCTTC 6 cut(s) 226, 590, 829, 1115, 1376, 1549
HpyCH4III ACNGT 1 cut(s) 1255
HpyCH4IV ACGT 2 cut(s) 1460, 1701
HpyF10VI GCNNNNNNNGC 3 cut(s) 242, 1148, 1667
HpyF3I CTNAG 6 cut(s) 228, 477, 1121, 1314, 1541, 1740
HpySE526I ACGT 2 cut(s) 1460, 1701
Hsp92II CATG 6 cut(s) 113, 319, 328, 383, 517, 960
Ksp22I TGATCA 1 cut(s) 36
KspI CCGCGG 1 cut(s) 727
Kzo9I GATC 7 cut(s) 36, 112, 678, 838, 984, 1128, 1279
LguI GCTCTTC 1 cut(s) 644
LmnI GCTCC 2 cut(s) 517, 1298
Lsp1109I GCAGC 9 cut(s) 182, 248, 251, 254, 276, 1182, 1313, 1541, 1718
LweI GCATC 3 cut(s) 652, 874, 1535
MabI ACCWGGT 1 cut(s) 610
MaeI CTAG 4 cut(s) 308, 743, 894, 1346
MaeII ACGT 2 cut(s) 1460, 1701
MaeIII GTNAC 4 cut(s) 365, 614, 786, 896
MalI GATC 7 cut(s) 38, 114, 680, 840, 986, 1130, 1281
MboI GATC 7 cut(s) 36, 112, 678, 838, 984, 1128, 1279
MflI RGATCY 2 cut(s) 838, 984
MhlI GDGCHC 2 cut(s) 153, 1122
MluCI AATT 6 cut(s) 338, 704, 827, 849, 1242, 1408
MlyI GAGTC 2 cut(s) 54, 1688
MmeI TCCRAC 2 cut(s) 235, 1359
Mph1103I ATGCAT 2 cut(s) 400, 1550
MseI TTAA 9 cut(s) 75, 138, 453, 549, 624, 734, 1011, 1497, 1713
MslI CAYNNNNRTG 1 cut(s) 1046
MspA1I CMGCKG 3 cut(s) 267, 726, 1195
MspCI CTTAAG 1 cut(s) 452
MspI CCGG 2 cut(s) 443, 953
MspR9I CCNGG 1 cut(s) 612
Mva1269I GAATGC 1 cut(s) 1548
MvaI CCWGG 1 cut(s) 612
MvnI CGCG 1 cut(s) 726
MwoI GCNNNNNNNGC 3 cut(s) 242, 1148, 1667
NcoI CCATGG 1 cut(s) 513
NdeII GATC 7 cut(s) 36, 112, 678, 838, 984, 1128, 1279
NlaIII CATG 6 cut(s) 113, 319, 328, 383, 517, 960
NlaIV GGNNCC 2 cut(s) 460, 840
NmuCI GTSAC 3 cut(s) 614, 786, 896
NsiI ATGCAT 2 cut(s) 400, 1550
NspI RCATGY 2 cut(s) 319, 960
PaeR7I CTCGAG 1 cut(s) 636
PagI TCATGA 1 cut(s) 109
PaqCI CACCTGC 1 cut(s) 1189
PciSI GCTCTTC 1 cut(s) 644
PctI GAATGC 1 cut(s) 1548
PfeI GAWTC 3 cut(s) 509, 539, 1397
PleI GAGTC 2 cut(s) 54, 1687
PmaCI CACGTG 1 cut(s) 1461
PmlI CACGTG 1 cut(s) 1461
PpsI GAGTC 2 cut(s) 54, 1687
Ppu21I YACGTR 1 cut(s) 1461
PshBI ATTAAT 1 cut(s) 734
PsiI TTATAA 1 cut(s) 1413
Psp124BI GAGCTC 1 cut(s) 1122
Psp6I CCWGG 1 cut(s) 610
PspCI CACGTG 1 cut(s) 1461
PspEI GGTNACC 1 cut(s) 614
PspGI CCWGG 1 cut(s) 610
PspN4I GGNNCC 2 cut(s) 460, 840
PstI CTGCAG 1 cut(s) 1200
PsuI RGATCY 2 cut(s) 838, 984
PvuII CAGCTG 2 cut(s) 267, 1195
RsaI GTAC 6 cut(s) 334, 710, 992, 1006, 1034, 1319
RsaNI GTAC 6 cut(s) 333, 709, 991, 1005, 1033, 1318
RseI CAYNNNNRTG 1 cut(s) 1046
SacI GAGCTC 1 cut(s) 1122
SacII CCGCGG 1 cut(s) 727
SapI GCTCTTC 1 cut(s) 644
SaqAI TTAA 9 cut(s) 75, 138, 453, 549, 624, 734, 1011, 1497, 1713
Sau3AI GATC 7 cut(s) 36, 112, 678, 838, 984, 1128, 1279
ScaI AGTACT 1 cut(s) 1006
SchI GAGTC 2 cut(s) 54, 1688
ScrFI CCNGG 1 cut(s) 612
SduI GDGCHC 2 cut(s) 153, 1122
SexAI ACCWGGT 1 cut(s) 610
SfaNI GCATC 3 cut(s) 652, 874, 1535
SfcI CTRYAG 3 cut(s) 64, 534, 1196
Sfr274I CTCGAG 1 cut(s) 636
Sfr303I CCGCGG 1 cut(s) 727
SgrBI CCGCGG 1 cut(s) 727
SlaI CTCGAG 1 cut(s) 636
SmiMI CAYNNNNRTG 1 cut(s) 1046
SmlI CTYRAG 4 cut(s) 80, 146, 452, 636
SmoI CTYRAG 4 cut(s) 80, 146, 452, 636
Sse9I AATT 6 cut(s) 338, 704, 827, 849, 1242, 1408
SsiI CCGC 4 cut(s) 577, 724, 726, 871
SspMI CTAG 4 cut(s) 308, 743, 894, 1346
SstI GAGCTC 1 cut(s) 1122
StyD4I CCNGG 1 cut(s) 610
StyI CCWWGG 2 cut(s) 513, 776
TaaI ACNGT 1 cut(s) 1255
TaiI ACGT 2 cut(s) 1463, 1704
TaqI TCGA 6 cut(s) 583, 637, 1101, 1474, 1612, 1724
TaqII GACCGA 1 cut(s) 1296
TasI AATT 6 cut(s) 338, 704, 827, 849, 1242, 1408
TatI WGTACW 4 cut(s) 332, 708, 1004, 1317
TauI GCSGC 1 cut(s) 579
TfiI GAWTC 3 cut(s) 509, 539, 1397
Tru1I TTAA 9 cut(s) 75, 138, 453, 549, 624, 734, 1011, 1497, 1713
Tru9I TTAA 9 cut(s) 75, 138, 453, 549, 624, 734, 1011, 1497, 1713
TscAI CASTG 3 cut(s) 100, 773, 1129
TseFI GTSAC 3 cut(s) 614, 786, 896
TseI GCWGC 9 cut(s) 170, 236, 239, 242, 264, 1195, 1301, 1529, 1706
Tsp45I GTSAC 3 cut(s) 614, 786, 896
TspGWI ACGGA 2 cut(s) 238, 899
TspRI CASTG 3 cut(s) 100, 773, 1129
Vha464I CTTAAG 1 cut(s) 452
VspI ATTAAT 1 cut(s) 734
XapI RAATTY 1 cut(s) 338
XbaI TCTAGA 1 cut(s) 307
XceI RCATGY 2 cut(s) 319, 960
XhoI CTCGAG 1 cut(s) 636
XspI CTAG 4 cut(s) 308, 743, 894, 1346
ZrmI AGTACT 1 cut(s) 1006
Zsp2I ATGCAT 2 cut(s) 400, 1550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.