Rroxscaffold_1G00008170

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000183
Physical Location & Seq
Forward (+)
10306485 .. 10314973
8489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00008170.1

Sequence Viewer

Length: 2523 bp
ATGGGTCGCCCCACTTTCTCCTCCGACGAAGTTGTGATCAAGTCTCCGAATGATAAGCGACTCTACAGATTGATTAAGCTTGAGAATGGCCTCACTGCATTGCTCATTCATGATCCTGATATATACCCACAAGGGTTTAAGAGCCTTGAGCCCTGTGAAGATGAAGAAGCAGCCAAAGGTGGAGATGGTGAGGTTGGAAATAAGGGAAAGGGAGGTGCTTTACGGACTAAGAAGGCAGCAGCAGCAATGTGCGTTGGAATAGGCAGCTGGTCTGACCCTATGGAGGCACAGGGGCTTGCACACTTTCTAGAGTTTAACAGGGTTCTGCAAAATGACTTTTGCCGCCTTCAACAACTTCAATGCTATACATCCACACCAGGTCACCCATTTAATAGATTTTCTCGAGAGTCGCTCGATGTACTAGAGAAGTGGATTTTGGAATTGTATAGAGATGTCAAAACCGGTCCCCAAGTAAATCTGGAGTTCAAGGCAGACGGTCCTATTTGGAAAGCTGGAAAAGTTTACAGGCTAGAGGCTGTTCAAGATGTTCATATACTGCAATTGTCATGGAAACTTCCATGCCTTCTTCAACACTATTTGAAATGCCCAGAATTTTATTTACATCATCTCCTTGTGCATGAGGGCAGGGGAAGTTTGCATTTCTACCTCAAAGCTAGAGGGTGGGCAACGTATCTAGGTGCTTCGGTGAGCCGCTATCCTGTGGCCGATGTCTTTTGCACGATCATATACCTCACTGACTCTGGACTGGAGAAGTGGGAAATATCAGTTAGTGTTGAACGATCTAATGAAGTCAACTTTACTCCATCTCTCACTTCTCAATTGCTATATGGAATGCTTATCTTGCAGATTTTTGAAATAATTGGGTTGGTGTATCAATACATTAAGTTACTACGTCAGGAGACGCCAGAATGGATATTTCGGGAACTCCAGGATATAGGGAACATGTGCTTTAGATTTGCAGAGGAGGAGCCTTTCCAGGATGATTATGTTTCAAACCTTGCAGAAAATTTACGACGTTATGCAGCAGAGCATGTTATTTATGGGGACTATGTGTTTGGGATATGGGATGAGGTATTGATACAAAATGTTCTGGGTTTCTTCTCTCCAGAAAACATGAGGATTGATGTGGTATCAAAGTTCTCATTTAAGTCAGAAGAGTTTCAGTGTGAGCCTTGGTTCGGGTCACATTATACCGAGGAAGATATATCTCCAGCTTTGATGGAATTGTGGAAGGATCCTCCCGAAATTGATGTATCGTTGCATCTGCGGGCAAAAAATGAGTTCATTCCTAGTGACTTTTCCATCCGTTCTGATGGGATGTGTCTTGATATTTCAAATATATCTTCTCCGGCATGTATGTTTGATGAACCATTGATGAAGATCTGGTACAAGCTTGATAGTACTTTTAAACTTCCACGGGCAAATGCGTACTTCTTCATCAGAATGAAAGGTGGATATGATAATGTAAAAAGTAGTGTTTTGACAGACTTATACATCGACCTTCTTGAAGATGAGCTCAGTGAGATCATCTATCAGGCTTATGTTGCTTGCTTGGGGACTTCTATTTCTGTGTTTACCGACAAACTCCAGCTGCAGGTGTATGGTTTCAATGATAAGCTTCCAGCTCTGTTGTCAAAAATTGTGGAAACAGTAAAGAGCTTCTTGCCAACTGATGATCGTTTTGAGGTTATTAAAGAAGACATGGTGCGAGTGTATAGGAATGCCAATACGAATCCTTGGGGTCACTCAACATACTTGAGAGAGCAAGTTTTGTTGCAGAGTTTCTACAACGTAGATGAGCTGTTTCATGTTTTGAATGGGTTGTCCGTTTCTGATTTGAAGTCATTTATTCCTGAGCTTTTCTCCCAGGCATATGCTTACAAAGTTACAATGTACTCTCCTCAATATGTACAGATCTACATTGAGGGCCTTTTCCATGGTAATTTGTCAGAAGAAGAAGCAATTAGCCTCTCAAATTTATTCAAGACAATCTTTTCCGTAAAACCACTTCCTGTTGAATTGATGTATAGAAATCATTGTATTTGTCTTCCTTCTAATGCTAACCTCATTAGAGATGCTACTGTGAAGAACAAGTCAGAAACAAACTCTGTGACAGAGGAAGGACTGGATGATGATTCCTTTGAGAATTATAAAGCTGGACAAATGGAAAATCTTTTGAAGAAAGATACATCCCTCACGTGTGAAACCAATCGATTGTGGAATGAGATTTATATTAAAAGGTATATGTTTGACTACTCAAAAGAGGCAGCAGAAGAACTCAGATGCATTCAGAAGGGGGATGTTATCAACTTTTACAAGACATATTTGCAACAATCATCTCCCAAGTGTCGAAGACTTGCAACTCGTGTTTGGGGTTGCAACACCGACTTCAAAGAGGCAGAAGTAGCACGACTGGAGTCTGTGCAAGTCATTGAAGACGTTGCAGCCTTTAAGATGTCATCGAAGTTCTATAAACCTAAGATAAATACTCTGGATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

840

Amino Acids

96.8

Weight (kDa)

5.18

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16_M PF16187 324 - 609 3e-81 Middle or third domain of peptidase_M16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2172
AarI CACCTGC 1 cut(s) 1606
Acc36I ACCTGC 1 cut(s) 1606
AciI CCGC 3 cut(s) 343, 712, 1288
AclWI GGATC 3 cut(s) 107, 1250, 1263
AcoI YGGCCR 1 cut(s) 723
AcsI RAATTY 3 cut(s) 611, 1027, 1996
AcvI CACGTG 1 cut(s) 2220
AcyI GRCGYC 1 cut(s) 923
AfaI GTAC 6 cut(s) 420, 1409, 1423, 1451, 1916, 1932
AfiI CCNNNNNNNGG 3 cut(s) 283, 1199, 1615
AflIII ACRYGT 2 cut(s) 963, 2219
AgeI ACCGGT 1 cut(s) 461
AjnI CCWGG 4 cut(s) 376, 948, 996, 1887
AjuI GAANNNNNNNTTGG 2 cut(s) 419, 451
Alw21I GWGCWC 1 cut(s) 1539
Alw26I GTCTC 2 cut(s) 48, 914
AlwI GGATC 3 cut(s) 107, 1250, 1263
Ama87I CYCGRG 1 cut(s) 402
AoxI GGCC 3 cut(s) 88, 723, 1948
ApeKI GCWGC 9 cut(s) 170, 236, 239, 242, 264, 1043, 1612, 2288, 2465
ApoI RAATTY 3 cut(s) 611, 1027, 1996
ArsI GACNNNNNNTTYG 2 cut(s) 1058, 1090
AsiGI ACCGGT 1 cut(s) 461
Asp700I GAANNNNTTC 1 cut(s) 1179
AspS9I GGNCC 3 cut(s) 464, 497, 1948
AsuHPI GGTGA 3 cut(s) 200, 374, 718
AvaI CYCGRG 1 cut(s) 402
AvaII GGWCC 2 cut(s) 464, 497
BamHI GGATCC 1 cut(s) 1255
BanII GRGCYC 2 cut(s) 153, 1539
BarI GAAGNNNNNNTAC 4 cut(s) 1391, 1423, 2013, 2045
BauI CACGAG 1 cut(s) 2385
BbrPI CACGTG 1 cut(s) 2220
BbsI GAAGAC 4 cut(s) 1725, 2060, 2380, 2463
Bbv12I GWGCWC 1 cut(s) 1539
BbvI GCAGC 9 cut(s) 182, 248, 251, 254, 276, 1055, 1599, 2300, 2477
BccI CCATC 5 cut(s) 179, 832, 1234, 1328, 1331
BciT130I CCWGG 4 cut(s) 378, 950, 998, 1889
BclI TGATCA 1 cut(s) 36
BcoDI GTCTC 2 cut(s) 48, 914
BfaI CTAG 6 cut(s) 308, 422, 530, 675, 695, 1311
BfmI CTRYAG 2 cut(s) 64, 1613
BfuAI ACCTGC 1 cut(s) 1606
BglII AGATCT 2 cut(s) 1401, 1935
BmcAI AGTACT 1 cut(s) 1423
Bme1390I CCNGG 4 cut(s) 378, 950, 998, 1889
Bme18I GGWCC 2 cut(s) 464, 497
BmeT110I CYCGRG 1 cut(s) 402
BmgT120I GGNCC 3 cut(s) 464, 497, 1948
BmiI GGNNCC 3 cut(s) 466, 990, 1257
BmrFI CCNGG 4 cut(s) 378, 950, 998, 1889
BmsI GCATC 4 cut(s) 1291, 2086, 2294, 2506
BoxI GACNNNNGTC 1 cut(s) 2437
BpiI GAAGAC 4 cut(s) 1725, 2060, 2380, 2463
BplI GAGNNNNNCTC 4 cut(s) 396, 428, 1868, 1900
BpmI CTGGAG 7 cut(s) 500, 788, 932, 1110, 1215, 1592, 2456
Bpu10I CCTNAGC 1 cut(s) 1875
BpuEI CTTGAG 3 cut(s) 101, 167, 1798
Bsa29I ATCGAT 1 cut(s) 2233
BsaAI YACGTR 1 cut(s) 2220
BsaBI GATNNNNATC 1 cut(s) 1400
BsaHI GRCGYC 1 cut(s) 923
BsaJI CCNNGG 6 cut(s) 1193, 1215, 1436, 1757, 1887, 1957
BsaWI WCCGGW 1 cut(s) 461
BsaXI ACNNNNNCTCC 2 cut(s) 612, 642
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 1199, 1615
Bse118I RCCGGY 1 cut(s) 461
Bse1I ACTGG 3 cut(s) 771, 2151, 2439
Bse3DI GCAATG 2 cut(s) 98, 252
Bse8I GATNNNNATC 1 cut(s) 1400
BseBI CCWGG 4 cut(s) 378, 950, 998, 1889
BseCI ATCGAT 1 cut(s) 2233
BseDI CCNNGG 6 cut(s) 1193, 1215, 1436, 1757, 1887, 1957
BseGI GGATG 9 cut(s) 368, 1006, 1093, 1323, 1344, 2155, 2210, 2326, 2521
BseJI GATNNNNATC 1 cut(s) 1400
BseLI CCNNNNNNNGG 3 cut(s) 283, 1199, 1615
BseMI GCAATG 2 cut(s) 98, 252
BseMII CTCAG 3 cut(s) 1552, 1866, 2314
BseNI ACTGG 3 cut(s) 771, 2151, 2439
BseRI GAGGAG 4 cut(s) 10, 998, 1001, 1911
BseXI GCAGC 9 cut(s) 182, 248, 251, 254, 276, 1055, 1599, 2300, 2477
BshFI GGCC 3 cut(s) 90, 725, 1950
BshTI ACCGGT 1 cut(s) 461
BshVI ATCGAT 1 cut(s) 2233
BsiHKAI GWGCWC 1 cut(s) 1539
BsiHKCI CYCGRG 1 cut(s) 402
BsiSI CCGG 2 cut(s) 462, 1370
BslFI GGGAC 3 cut(s) 450, 1079, 1591
BslI CCNNNNNNNGG 3 cut(s) 283, 1199, 1615
BsmAI GTCTC 2 cut(s) 48, 914
BsmBI CGTCTC 1 cut(s) 914
BsmFI GGGAC 3 cut(s) 450, 1079, 1591
BsmI GAATGC 3 cut(s) 858, 1747, 2307
BsnI GGCC 3 cut(s) 90, 725, 1950
BsoBI CYCGRG 1 cut(s) 402
Bsp1286I GDGCHC 2 cut(s) 153, 1539
Bsp1407I TGTACA 1 cut(s) 1930
Bsp143I GATC 9 cut(s) 36, 112, 741, 800, 1255, 1401, 1545, 1696, 1935
Bsp19I CCATGG 1 cut(s) 1957
BspACI CCGC 3 cut(s) 343, 712, 1288
BspANI GGCC 3 cut(s) 90, 725, 1950
BspCNI CTCAG 3 cut(s) 1551, 1867, 2313
BspDI ATCGAT 1 cut(s) 2233
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 3 cut(s) 466, 990, 1257
BspMAI CTGCAG 1 cut(s) 1617
BspMI ACCTGC 1 cut(s) 1606
BspPI GGATC 3 cut(s) 107, 1250, 1263
BsrDI GCAATG 2 cut(s) 98, 252
BsrFI RCCGGY 1 cut(s) 461
BsrGI TGTACA 1 cut(s) 1930
BsrI ACTGG 3 cut(s) 771, 2151, 2439
BssAI RCCGGY 1 cut(s) 461
BssECI CCNNGG 6 cut(s) 1193, 1215, 1436, 1757, 1887, 1957
BssMI GATC 9 cut(s) 36, 112, 741, 800, 1255, 1401, 1545, 1696, 1935
BssNI GRCGYC 1 cut(s) 923
BssSI CACGAG 1 cut(s) 2385
BssT1I CCWWGG 3 cut(s) 1193, 1757, 1957
Bst2BI CACGAG 1 cut(s) 2385
Bst2UI CCWGG 4 cut(s) 378, 950, 998, 1889
Bst4CI ACNGT 3 cut(s) 497, 1672, 2104
Bst6I CTCTTC 1 cut(s) 1170
BstACI GRCGYC 1 cut(s) 923
BstAUI TGTACA 1 cut(s) 1930
BstBAI YACGTR 1 cut(s) 2220
BstC8I GCNNGC 3 cut(s) 297, 1290, 1570
BstDEI CTNAG 5 cut(s) 228, 1538, 1875, 2300, 2499
BstDSI CCRYGG 2 cut(s) 1436, 1957
BstEII GGTNACC 1 cut(s) 380
BstF5I GGATG 9 cut(s) 368, 1006, 1093, 1323, 1344, 2155, 2210, 2326, 2521
BstKTI GATC 9 cut(s) 39, 115, 744, 803, 1258, 1404, 1548, 1699, 1938
BstMAI GTCTC 2 cut(s) 48, 914
BstMBI GATC 9 cut(s) 36, 112, 741, 800, 1255, 1401, 1545, 1696, 1935
BstMWI GCNNNNNNNGC 4 cut(s) 242, 862, 1565, 2426
BstNI CCWGG 4 cut(s) 378, 950, 998, 1889
BstNSI RCATGY 3 cut(s) 967, 1055, 1377
BstPAI GACNNNNGTC 1 cut(s) 2437
BstPI GGTNACC 1 cut(s) 380
BstSCI CCNGG 4 cut(s) 376, 948, 996, 1887
BstSFI CTRYAG 2 cut(s) 64, 1613
BstV1I GCAGC 9 cut(s) 182, 248, 251, 254, 276, 1055, 1599, 2300, 2477
BstV2I GAAGAC 4 cut(s) 1725, 2060, 2380, 2463
BstX2I RGATCY 3 cut(s) 1255, 1401, 1935
BstYI RGATCY 3 cut(s) 1255, 1401, 1935
Bsu15I ATCGAT 1 cut(s) 2233
BsuRI GGCC 3 cut(s) 90, 725, 1950
BsuTUI ATCGAT 1 cut(s) 2233
BtgI CCRYGG 2 cut(s) 1436, 1957
BtsCI GGATG 9 cut(s) 368, 1006, 1093, 1323, 1344, 2155, 2210, 2326, 2521
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 4 cut(s) 93, 753, 1190, 1546
BveI ACCTGC 1 cut(s) 1606
Cac8I GCNNGC 3 cut(s) 297, 1290, 1570
CciI TCATGA 1 cut(s) 109
Cfr10I RCCGGY 1 cut(s) 461
Cfr13I GGNCC 3 cut(s) 464, 497, 1948
ClaI ATCGAT 1 cut(s) 2233
CseI GACGC 1 cut(s) 931
CsiI ACCWGGT 1 cut(s) 376
Csp6I GTAC 6 cut(s) 419, 1408, 1422, 1450, 1915, 1931
CspAI ACCGGT 1 cut(s) 461
CspCI CAANNNNNGTGG 2 cut(s) 1644, 1679
CviQI GTAC 6 cut(s) 419, 1408, 1422, 1450, 1915, 1931
DdeI CTNAG 5 cut(s) 228, 1538, 1875, 2300, 2499
DpnI GATC 9 cut(s) 38, 114, 743, 802, 1257, 1403, 1547, 1698, 1937
DpnII GATC 9 cut(s) 36, 112, 741, 800, 1255, 1401, 1545, 1696, 1935
DraI TTTAAA 1 cut(s) 1429
EaeI YGGCCR 1 cut(s) 723
Eam1104I CTCTTC 1 cut(s) 1170
EarI CTCTTC 1 cut(s) 1170
Ecl136II GAGCTC 1 cut(s) 1537
Eco130I CCWWGG 3 cut(s) 1193, 1757, 1957
Eco24I GRGCYC 2 cut(s) 153, 1539
Eco47I GGWCC 2 cut(s) 464, 497
Eco53kI GAGCTC 1 cut(s) 1537
Eco72I CACGTG 1 cut(s) 2220
Eco88I CYCGRG 1 cut(s) 402
Eco91I GGTNACC 1 cut(s) 380
EcoICRI GAGCTC 1 cut(s) 1537
EcoO109I RGGNCCY 1 cut(s) 1948
EcoO65I GGTNACC 1 cut(s) 380
EcoRII CCWGG 4 cut(s) 376, 948, 996, 1887
EcoT14I CCWWGG 3 cut(s) 1193, 1757, 1957
EcoT22I ATGCAT 1 cut(s) 2309
EcoT38I GRGCYC 2 cut(s) 153, 1539
ErhI CCWWGG 3 cut(s) 1193, 1757, 1957
Esp3I CGTCTC 1 cut(s) 914
FalI AAGNNNNNCTT 4 cut(s) 1667, 1699, 1997, 2029
FaqI GGGAC 3 cut(s) 450, 1079, 1591
FauI CCCGC 1 cut(s) 1281
FauNDI CATATG 1 cut(s) 1894
FbaI TGATCA 1 cut(s) 36
FokI GGATG 8 cut(s) 355, 1013, 1100, 1310, 1351, 2162, 2197, 2333
FriOI GRGCYC 2 cut(s) 153, 1539
FspBI CTAG 6 cut(s) 308, 422, 530, 675, 695, 1311
GsuI CTGGAG 7 cut(s) 500, 788, 932, 1110, 1215, 1592, 2456
HaeIII GGCC 3 cut(s) 90, 725, 1950
HapII CCGG 2 cut(s) 462, 1370
HgaI GACGC 1 cut(s) 931
Hin1I GRCGYC 1 cut(s) 923
HincII GTYRAC 1 cut(s) 814
HindII GTYRAC 1 cut(s) 814
HindIII AAGCTT 3 cut(s) 77, 1412, 1637
HinfI GANTC 6 cut(s) 60, 407, 758, 1753, 2156, 2438
HpaII CCGG 2 cut(s) 462, 1370
HphI GGTGA 3 cut(s) 200, 374, 718
Hpy166II GTNNAC 3 cut(s) 523, 814, 1596
Hpy8I GTNNAC 3 cut(s) 523, 814, 1596
Hpy99I CGWCG 2 cut(s) 29, 1038
HpyAV CCTTC 8 cut(s) 226, 356, 593, 1246, 1532, 2082, 2135, 2308
HpyCH4III ACNGT 3 cut(s) 497, 1672, 2104
HpyCH4IV ACGT 6 cut(s) 689, 913, 1036, 1812, 2219, 2460
HpyF10VI GCNNNNNNNGC 4 cut(s) 242, 862, 1565, 2426
HpyF3I CTNAG 5 cut(s) 228, 1538, 1875, 2300, 2499
HpySE526I ACGT 6 cut(s) 689, 913, 1036, 1812, 2219, 2460
Hsp92I GRCGYC 1 cut(s) 923
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 9 cut(s) 36, 112, 741, 800, 1255, 1401, 1545, 1696, 1935
LmnI GCTCC 1 cut(s) 988
Lsp1109I GCAGC 9 cut(s) 182, 248, 251, 254, 276, 1055, 1599, 2300, 2477
LweI GCATC 4 cut(s) 1291, 2086, 2294, 2506
MabI ACCWGGT 1 cut(s) 376
MaeI CTAG 6 cut(s) 308, 422, 530, 675, 695, 1311
MaeII ACGT 6 cut(s) 689, 913, 1036, 1812, 2219, 2460
MaeIII GTNAC 7 cut(s) 380, 906, 1203, 1313, 1763, 1906, 2131
MalI GATC 9 cut(s) 38, 114, 743, 802, 1257, 1403, 1547, 1698, 1937
MboI GATC 9 cut(s) 36, 112, 741, 800, 1255, 1401, 1545, 1696, 1935
MfeI CAATTG 2 cut(s) 560, 839
MflI RGATCY 3 cut(s) 1255, 1401, 1935
MhlI GDGCHC 2 cut(s) 153, 1539
MlyI GAGTC 4 cut(s) 54, 416, 752, 2447
MmeI TCCRAC 3 cut(s) 48, 175, 235
Mph1103I ATGCAT 1 cut(s) 2309
MroXI GAANNNNTTC 1 cut(s) 1179
MslI CAYNNNNRTG 1 cut(s) 1463
MspA1I CMGCKG 2 cut(s) 267, 1612
MspI CCGG 2 cut(s) 462, 1370
MspR9I CCNGG 4 cut(s) 378, 950, 998, 1889
MunI CAATTG 2 cut(s) 560, 839
Mva1269I GAATGC 3 cut(s) 858, 1747, 2307
MvaI CCWGG 4 cut(s) 378, 950, 998, 1889
MwoI GCNNNNNNNGC 4 cut(s) 242, 862, 1565, 2426
NcoI CCATGG 1 cut(s) 1957
NdeI CATATG 1 cut(s) 1894
NdeII GATC 9 cut(s) 36, 112, 741, 800, 1255, 1401, 1545, 1696, 1935
NlaIV GGNNCC 3 cut(s) 466, 990, 1257
NmuCI GTSAC 5 cut(s) 380, 1203, 1313, 1763, 2131
NsiI ATGCAT 1 cut(s) 2309
NspI RCATGY 3 cut(s) 967, 1055, 1377
PaeR7I CTCGAG 1 cut(s) 402
PagI TCATGA 1 cut(s) 109
PaqCI CACCTGC 1 cut(s) 1606
PciI ACATGT 1 cut(s) 963
PctI GAATGC 3 cut(s) 858, 1747, 2307
PdmI GAANNNNTTC 1 cut(s) 1179
PfeI GAWTC 2 cut(s) 1753, 2156
PfoI TCCNGGA 2 cut(s) 948, 996
PinAI ACCGGT 1 cut(s) 461
PleI GAGTC 4 cut(s) 54, 415, 752, 2446
PmaCI CACGTG 1 cut(s) 2220
PmlI CACGTG 1 cut(s) 2220
PpsI GAGTC 4 cut(s) 54, 415, 752, 2446
Ppu21I YACGTR 1 cut(s) 2220
PscI ACATGT 1 cut(s) 963
PshAI GACNNNNGTC 1 cut(s) 2437
PsiI TTATAA 1 cut(s) 2172
Psp124BI GAGCTC 1 cut(s) 1539
Psp6I CCWGG 4 cut(s) 376, 948, 996, 1887
PspCI CACGTG 1 cut(s) 2220
PspEI GGTNACC 1 cut(s) 380
PspGI CCWGG 4 cut(s) 376, 948, 996, 1887
PspN4I GGNNCC 3 cut(s) 466, 990, 1257
PspPI GGNCC 3 cut(s) 464, 497, 1948
PstI CTGCAG 1 cut(s) 1617
PsuI RGATCY 3 cut(s) 1255, 1401, 1935
PvuII CAGCTG 2 cut(s) 267, 1612
RsaI GTAC 6 cut(s) 420, 1409, 1423, 1451, 1916, 1932
RsaNI GTAC 6 cut(s) 419, 1408, 1422, 1450, 1915, 1931
RseI CAYNNNNRTG 1 cut(s) 1463
SacI GAGCTC 1 cut(s) 1539
Sau3AI GATC 9 cut(s) 36, 112, 741, 800, 1255, 1401, 1545, 1696, 1935
Sau96I GGNCC 3 cut(s) 464, 497, 1948
ScaI AGTACT 1 cut(s) 1423
SchI GAGTC 4 cut(s) 54, 416, 752, 2447
ScrFI CCNGG 4 cut(s) 378, 950, 998, 1889
SduI GDGCHC 2 cut(s) 153, 1539
SexAI ACCWGGT 1 cut(s) 376
SfaNI GCATC 4 cut(s) 1291, 2086, 2294, 2506
SfcI CTRYAG 2 cut(s) 64, 1613
Sfr274I CTCGAG 1 cut(s) 402
SinI GGWCC 2 cut(s) 464, 497
SlaI CTCGAG 1 cut(s) 402
SmiMI CAYNNNNRTG 1 cut(s) 1463
SmlI CTYRAG 4 cut(s) 80, 146, 402, 1777
SmoI CTYRAG 4 cut(s) 80, 146, 402, 1777
SsiI CCGC 3 cut(s) 343, 712, 1288
SspMI CTAG 6 cut(s) 308, 422, 530, 675, 695, 1311
SstI GAGCTC 1 cut(s) 1539
StyD4I CCNGG 4 cut(s) 376, 948, 996, 1887
StyI CCWWGG 3 cut(s) 1193, 1757, 1957
TaaI ACNGT 3 cut(s) 497, 1672, 2104
TaiI ACGT 6 cut(s) 692, 916, 1039, 1815, 2222, 2463
TaqI TCGA 6 cut(s) 403, 414, 1518, 2233, 2371, 2483
TatI WGTACW 4 cut(s) 418, 1421, 1914, 1930
TauI GCSGC 2 cut(s) 345, 714
TfiI GAWTC 2 cut(s) 1753, 2156
TscAI CASTG 4 cut(s) 100, 760, 1190, 1546
TseFI GTSAC 5 cut(s) 380, 1203, 1313, 1763, 2131
TseI GCWGC 9 cut(s) 170, 236, 239, 242, 264, 1043, 1612, 2288, 2465
Tsp45I GTSAC 5 cut(s) 380, 1203, 1313, 1763, 2131
TspGWI ACGGA 4 cut(s) 238, 1316, 1837, 2008
TspRI CASTG 4 cut(s) 100, 760, 1190, 1546
VpaK11BI GGWCC 2 cut(s) 464, 497
XapI RAATTY 3 cut(s) 611, 1027, 1996
XbaI TCTAGA 1 cut(s) 307
XceI RCATGY 3 cut(s) 967, 1055, 1377
XhoI CTCGAG 1 cut(s) 402
XmnI GAANNNNTTC 1 cut(s) 1179
XspI CTAG 6 cut(s) 308, 422, 530, 675, 695, 1311
ZrmI AGTACT 1 cut(s) 1423
Zsp2I ATGCAT 1 cut(s) 2309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.