Rh5BG497700

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000183
Physical Location & Seq
Reverse (-)
79315627 .. 79322560
6934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG497700.1

Sequence Viewer

Length: 2145 bp
ATGGGTCGCTGCACTTTCTCCTCGGACGATATTGTAATAAAGTCTCCGAATGATAAGAAATTGTACAGACTAATTGAGCTTGAGAATGGCCTCACTGCATTGCTCATTCATGATCCTGAGATTTACCCACAAGGGCCCAAAAGCCTTGAACCCAGTGAAGAAGATGAAGCAGCCAAAGGTGGAGATGGTGCCTCTGAGACTAAGACGGCAGCAGCAGGAATGTGCGTTGGAATAGGCAGCATGTCTGACCCTGTTGAGGCACAAGGGCTTGCACACTTCCTAGAACACATGCTCTTTATGGGGAGTACAAAGTTTCCTATTGAAAATGAGTTCGGAAGTTACTTGTCCAAGCATGGAGGATGGTCAAATGCATACACAGAAGCAGAGCAGACTTGCTACTATTTTGTTGTAAAACGAGAGTTTCTAAAGGGTGCCTTATCAAGATTTTCTCAGTTCTTTGTTTCACCTCTAATGAAAAGAGAAGCCATGGAGCGAGAGTTACAGGCTATAGATTCAGAGGGCAGGGGAAGTTTGTATTTCCACCTCAAAGCGAGAGGTTGGGCAACATTTGTAAGTGCTCCAGTGGAACACTATTCTGTGGCTGATGTCTTCTGCATGATCATATACCTCACTGACTCTGGATTGGAGAAGATTTTCGATATAATTGGGTTGGTGTATCAATACATTAAGTTCTTACGGCAAGTGTTGCCCCAAGAATGGATATTTCGTGAACTCCAGGATATTGGGAACATCTACCTTAAGTTTTTAGAGGAGCAGCCCCAGGATTATTATGCTTTAAGACTTGGAGGAAATTTACTACATTATCCCGCAGAGCATGCTATTTATGGGGACTATTTGCTAGAGAATTGGGATGAGAAATTGATAGAATATACCCTAGGTTTCTTGAGACCAGAAAACATGAGGATTGATGTGATATCTAAGTCCTCCACATTGTCAGAAGATTTCCAGTGCGAGCCTTGGTTTGGGTCACATTTTACCGAGGAAGATATATCTCCGTCTTTGATAGATTTATGGAAGGATCCTCAAGAAATTGATAGTTCATTGCATCTCCCAGAAAAGAATGAATTCATTCCCTCTGATTTTTCCATCCGTTCTGATGTTCTTGATACCGCAAATACATCTTGTCCTAGATGTATACTTGATGGGCCATTGGTGAAGTTATGGTACAAGCTTGATAGTACTTACAAACTTCCGCAGGCAAACATATACTTTCGCGTCAGTCTAAAGGAAGCATCTGGTAGTGTGAAGAGTTCTGTCTTGACTCAACTATATGGCGACCTTCTTTGGGATGAGCTGAATGAGGTTGTATATCAGGCTAGTGTCGCCCAGCTGGGAACTTCTGTATCTGTGTACACTAACTATCTGGAGCTGAAGGTCAGCGGTTTCAATGATAAGATTCCAACTCTCTTGTCAAAAATTATGACAACAGCCAAAAATTTCTCGCCAACTTACGAATGTTTCAAGGTTATTAAAGAGGACATGGAGCGAGCATATAAGAACACCAATATGGATCCTTGGAGCCACTCGACATACTTGAGAGATGAAGTTTTGTACCAAAATTTCTGTGATGTAGATGAGACGTTGCATGTTTTAAATGGATTGACTGTTTCTGATGTGAAGTCATTCATTCCCGAGCTTTGGTCCCCGTCGTCTGATTATAATCCAATCTACTTACAAAGAAGAGTTGACAACTTTATCAATGGTGTGGAAGAGCTTTTGCTAGGATTGGATGATGAATCCTTTGAGAATTACCAAGGTGGGTTAATAGCGAAGCTATTGGAGAAAGATTCAACCCTTATGGATGAAACTGCTCGATATTGGGATCATATTATCTGCAAAAGGTATAAGTTTGACAAATGGGAAAAGATAGCAGAAGAACTCAGATGTCTACAGAAGGGGGATGTTATCAACTTTTACAAGACTTACTTGCAACTATCATCTCCCAAGCGTCGGAGACTTGTAACTCGTGTTTGGGGTTGCAACACTGACATGAAAGAAGCTGAAGAAGCGCGAACGGAGTCTGAGCAAATCATTGAAGACCTTGCAGCCTTTAAGAGGTCATCTCAGTTCTATGGTCATGGAAGTTGTCAGACAGTTGCTCCTCCGATTATGTGTAAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

714

Amino Acids

81.95

Weight (kDa)

4.9

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 66 - 187 9.6e-31 Insulinase (Peptidase family M16)
Peptidase_M16_M PF16187 254 - 536 1.7e-81 Middle or third domain of peptidase_M16
PqqF-like_C_4 PF22456 557 - 614 2.8e-07 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1680
AccB1I GGYRCC 2 cut(s) 188, 431
AccI GTMKAC 2 cut(s) 1156, 1909
AccII CGCG 2 cut(s) 1236, 2032
AciI CCGC 4 cut(s) 828, 1131, 1214, 1401
AclWI GGATC 6 cut(s) 107, 1034, 1047, 1526, 1539, 1851
AcsI RAATTY 4 cut(s) 811, 1085, 1456, 1579
AcuI CTGAAG 2 cut(s) 1412, 2043
AfaI GTAC 6 cut(s) 65, 307, 1187, 1201, 1373, 1574
AfiI CCNNNNNNNGG 7 cut(s) 256, 717, 983, 1306, 1659, 1971, 2076
AflII CTTAAG 1 cut(s) 758
AgsI TTSAA 6 cut(s) 149, 323, 1408, 1483, 1812, 2057
AjnI CCWGG 2 cut(s) 735, 780
Alw21I GWGCWC 1 cut(s) 580
Alw26I GTCTC 5 cut(s) 48, 191, 901, 1592, 1969
AlwI GGATC 6 cut(s) 107, 1034, 1047, 1526, 1539, 1851
Ama87I CYCGRG 1 cut(s) 1652
AoxI GGCC 3 cut(s) 88, 134, 1166
ApaI GGGCCC 1 cut(s) 138
ApeKI GCWGC 7 cut(s) 9, 170, 209, 212, 237, 775, 2066
ApoI RAATTY 4 cut(s) 811, 1085, 1456, 1579
Asp700I GAANNNNTTC 4 cut(s) 653, 1085, 1089, 1643
AspA2I CCTAGG 1 cut(s) 895
AspLEI GCGC 1 cut(s) 2032
AspS9I GGNCC 4 cut(s) 134, 135, 1166, 1662
AsuHPI GGTGA 2 cut(s) 456, 1186
AvaI CYCGRG 1 cut(s) 1652
AvaII GGWCC 1 cut(s) 1662
AvrII CCTAGG 1 cut(s) 895
BaeGI GKGCMC 1 cut(s) 138
BaeI ACNNNNGTAYC 2 cut(s) 1347, 1380
BamHI GGATCC 2 cut(s) 1039, 1531
BanI GGYRCC 2 cut(s) 188, 431
BanII GRGCYC 1 cut(s) 138
BarI GAAGNNNNNNTAC 2 cut(s) 1169, 1201
BauI CACGAG 1 cut(s) 1986
BbsI GAAGAC 2 cut(s) 601, 2064
Bbv12I GWGCWC 1 cut(s) 580
BbvI GCAGC 6 cut(s) 182, 221, 224, 249, 787, 2078
BccI CCATC 4 cut(s) 179, 354, 1115, 1157
BceAI ACGGC 2 cut(s) 222, 713
BciT130I CCWGG 2 cut(s) 737, 782
BclI TGATCA 1 cut(s) 618
BcoDI GTCTC 5 cut(s) 48, 191, 901, 1592, 1969
BfaI CTAG 6 cut(s) 281, 860, 896, 1149, 1338, 1742
BfmI CTRYAG 2 cut(s) 507, 1910
BfrI CTTAAG 1 cut(s) 758
BisI GCNGC 7 cut(s) 10, 171, 210, 213, 238, 776, 2067
BlnI CCTAGG 1 cut(s) 895
BlsI GCNGC 7 cut(s) 11, 172, 211, 214, 239, 777, 2068
BmcAI AGTACT 1 cut(s) 1201
Bme1390I CCNGG 2 cut(s) 737, 782
Bme18I GGWCC 1 cut(s) 1662
BmeT110I CYCGRG 1 cut(s) 1652
BmgT120I GGNCC 4 cut(s) 134, 135, 1166, 1662
BmiI GGNNCC 7 cut(s) 136, 190, 433, 1041, 1533, 1541, 1664
BmrFI CCNGG 2 cut(s) 737, 782
BmrI ACTGGG 1 cut(s) 147
BmsI GCATC 2 cut(s) 1075, 1262
BmuI ACTGGG 1 cut(s) 147
BpiI GAAGAC 2 cut(s) 601, 2064
BplI GAGNNNNNCTC 2 cut(s) 2068, 2100
BpmI CTGGAG 3 cut(s) 564, 719, 1406
BpuEI CTTGAG 4 cut(s) 101, 925, 1029, 1576
BsaBI GATNNNNATC 1 cut(s) 1680
BsaI GGTCTC 1 cut(s) 901
BsaJI CCNNGG 8 cut(s) 21, 486, 780, 895, 977, 999, 1535, 1774
BsaXI ACNNNNNCTCC 4 cut(s) 482, 512, 2104, 2134
Bsc4I CCNNNNNNNGG 7 cut(s) 256, 717, 983, 1306, 1659, 1971, 2076
Bse1I ACTGG 3 cut(s) 153, 581, 967
Bse3DI GCAATG 2 cut(s) 98, 1061
Bse8I GATNNNNATC 1 cut(s) 1680
BseBI CCWGG 2 cut(s) 737, 782
BseDI CCNNGG 8 cut(s) 21, 486, 780, 895, 977, 999, 1535, 1774
BseGI GGATG 7 cut(s) 365, 877, 1107, 1315, 1756, 1828, 1927
BseJI GATNNNNATC 1 cut(s) 1680
BseLI CCNNNNNNNGG 7 cut(s) 256, 717, 983, 1306, 1659, 1971, 2076
BseMI GCAATG 2 cut(s) 98, 1061
BseMII CTCAG 6 cut(s) 108, 186, 464, 1915, 2034, 2099
BseNI ACTGG 3 cut(s) 153, 581, 967
BseRI GAGGAG 3 cut(s) 10, 785, 2112
BseSI GKGCMC 1 cut(s) 138
BseXI GCAGC 6 cut(s) 182, 221, 224, 249, 787, 2078
BseYI CCCAGC 2 cut(s) 1347, 1351
Bsh1236I CGCG 2 cut(s) 1236, 2032
BshFI GGCC 3 cut(s) 90, 136, 1168
BshNI GGYRCC 2 cut(s) 188, 431
BsiHKAI GWGCWC 1 cut(s) 580
BsiHKCI CYCGRG 1 cut(s) 1652
BslFI GGGAC 2 cut(s) 863, 1648
BslI CCNNNNNNNGG 7 cut(s) 256, 717, 983, 1306, 1659, 1971, 2076
BsmAI GTCTC 5 cut(s) 48, 191, 901, 1592, 1969
BsmBI CGTCTC 1 cut(s) 1592
BsmFI GGGAC 2 cut(s) 863, 1648
BsnI GGCC 3 cut(s) 90, 136, 1168
Bso31I GGTCTC 1 cut(s) 901
BsoBI CYCGRG 1 cut(s) 1652
Bsp120I GGGCCC 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 138, 580
Bsp1407I TGTACA 2 cut(s) 63, 1371
Bsp143I GATC 5 cut(s) 112, 618, 1039, 1531, 1843
Bsp19I CCATGG 1 cut(s) 486
BspACI CCGC 4 cut(s) 828, 1131, 1214, 1401
BspANI GGCC 3 cut(s) 90, 136, 1168
BspCNI CTCAG 6 cut(s) 109, 187, 463, 1914, 2035, 2098
BspFNI CGCG 2 cut(s) 1236, 2032
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 7 cut(s) 136, 190, 433, 1041, 1533, 1541, 1664
BspPI GGATC 6 cut(s) 107, 1034, 1047, 1526, 1539, 1851
BspQI GCTCTTC 1 cut(s) 1725
BspT107I GGYRCC 2 cut(s) 188, 431
BspTI CTTAAG 1 cut(s) 758
BspTNI GGTCTC 1 cut(s) 901
BsrDI GCAATG 2 cut(s) 98, 1061
BsrGI TGTACA 2 cut(s) 63, 1371
BsrI ACTGG 3 cut(s) 153, 581, 967
BssECI CCNNGG 8 cut(s) 21, 486, 780, 895, 977, 999, 1535, 1774
BssMI GATC 5 cut(s) 112, 618, 1039, 1531, 1843
BssNAI GTATAC 1 cut(s) 1157
BssSI CACGAG 1 cut(s) 1986
BssT1I CCWWGG 5 cut(s) 486, 895, 977, 1535, 1774
Bst1107I GTATAC 1 cut(s) 1157
Bst2BI CACGAG 1 cut(s) 1986
Bst2UI CCWGG 2 cut(s) 737, 782
Bst4CI ACNGT 2 cut(s) 1627, 2116
Bst6I CTCTTC 3 cut(s) 1262, 1696, 1725
BstAFI CTTAAG 1 cut(s) 758
BstAPI GCANNNNNTGC 2 cut(s) 706, 836
BstAUI TGTACA 2 cut(s) 63, 1371
BstC8I GCNNGC 5 cut(s) 270, 837, 974, 1218, 1509
BstDEI CTNAG 8 cut(s) 117, 195, 201, 450, 939, 1901, 2043, 2085
BstDSI CCRYGG 1 cut(s) 486
BstENI CCTNNNNNAGG 1 cut(s) 2074
BstF5I GGATG 7 cut(s) 365, 877, 1107, 1315, 1756, 1828, 1927
BstFNI CGCG 2 cut(s) 1236, 2032
BstHHI GCGC 1 cut(s) 2032
BstKTI GATC 5 cut(s) 115, 621, 1042, 1534, 1846
BstMAI GTCTC 5 cut(s) 48, 191, 901, 1592, 1969
BstMBI GATC 5 cut(s) 112, 618, 1039, 1531, 1843
BstMWI GCNNNNNNNGC 4 cut(s) 706, 836, 1343, 2027
BstNI CCWGG 2 cut(s) 737, 782
BstNSI RCATGY 4 cut(s) 244, 292, 839, 1610
BstSCI CCNGG 2 cut(s) 735, 780
BstSFI CTRYAG 2 cut(s) 507, 1910
BstSLI GKGCMC 1 cut(s) 138
BstUI CGCG 2 cut(s) 1236, 2032
BstV1I GCAGC 6 cut(s) 182, 221, 224, 249, 787, 2078
BstV2I GAAGAC 2 cut(s) 601, 2064
BstX2I RGATCY 2 cut(s) 1039, 1531
BstXI CCANNNNNNTGG 1 cut(s) 743
BstYI RGATCY 2 cut(s) 1039, 1531
BstZ17I GTATAC 1 cut(s) 1157
BsuRI GGCC 3 cut(s) 90, 136, 1168
BtgI CCRYGG 1 cut(s) 486
BtsCI GGATG 7 cut(s) 365, 877, 1107, 1315, 1756, 1828, 1927
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 6 cut(s) 93, 160, 588, 630, 974, 2004
Cac8I GCNNGC 5 cut(s) 270, 837, 974, 1218, 1509
CciI TCATGA 1 cut(s) 109
CfoI GCGC 1 cut(s) 2032
Cfr13I GGNCC 4 cut(s) 134, 135, 1166, 1662
CseI GACGC 2 cut(s) 1225, 1958
Csp6I GTAC 6 cut(s) 64, 306, 1186, 1200, 1372, 1573
CviQI GTAC 6 cut(s) 64, 306, 1186, 1200, 1372, 1573
DdeI CTNAG 8 cut(s) 117, 195, 201, 450, 939, 1901, 2043, 2085
DpnI GATC 5 cut(s) 114, 620, 1041, 1533, 1845
DpnII GATC 5 cut(s) 112, 618, 1039, 1531, 1843
DraI TTTAAA 1 cut(s) 1614
Eam1104I CTCTTC 3 cut(s) 1262, 1696, 1725
EarI CTCTTC 3 cut(s) 1262, 1696, 1725
Eco130I CCWWGG 5 cut(s) 486, 895, 977, 1535, 1774
Eco24I GRGCYC 1 cut(s) 138
Eco31I GGTCTC 1 cut(s) 901
Eco32I GATATC 1 cut(s) 936
Eco47I GGWCC 1 cut(s) 1662
Eco57I CTGAAG 2 cut(s) 1412, 2043
Eco88I CYCGRG 1 cut(s) 1652
EcoNI CCTNNNNNAGG 1 cut(s) 2074
EcoO109I RGGNCCY 1 cut(s) 134
EcoRI GAATTC 1 cut(s) 1085
EcoRII CCWGG 2 cut(s) 735, 780
EcoRV GATATC 1 cut(s) 936
EcoT14I CCWWGG 5 cut(s) 486, 895, 977, 1535, 1774
EcoT22I ATGCAT 1 cut(s) 373
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 5 cut(s) 486, 895, 977, 1535, 1774
Esp3I CGTCTC 1 cut(s) 1592
FalI AAGNNNNNCTT 4 cut(s) 419, 451, 1931, 1963
FaqI GGGAC 2 cut(s) 863, 1648
FauI CCCGC 1 cut(s) 835
FbaI TGATCA 1 cut(s) 618
FblI GTMKAC 2 cut(s) 1156, 1909
Fnu4HI GCNGC 7 cut(s) 10, 171, 210, 213, 238, 776, 2067
FokI GGATG 7 cut(s) 372, 884, 1094, 1322, 1763, 1835, 1934
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 7 cut(s) 10, 171, 210, 213, 238, 776, 2067
FspBI CTAG 6 cut(s) 281, 860, 896, 1149, 1338, 1742
GlaI GCGC 1 cut(s) 2031
GluI GCNGC 7 cut(s) 10, 171, 210, 213, 238, 776, 2067
GsaI CCCAGC 2 cut(s) 1351, 1355
GsuI CTGGAG 3 cut(s) 564, 719, 1406
HaeIII GGCC 3 cut(s) 90, 136, 1168
HgaI GACGC 2 cut(s) 1225, 1958
HhaI GCGC 1 cut(s) 2032
Hin6I GCGC 1 cut(s) 2030
HinP1I GCGC 1 cut(s) 2030
HincII GTYRAC 1 cut(s) 1708
HindII GTYRAC 1 cut(s) 1708
HindIII AAGCTT 2 cut(s) 1190, 2138
HinfI GANTC 7 cut(s) 512, 635, 1282, 1417, 1757, 1808, 2039
HphI GGTGA 2 cut(s) 456, 1186
Hpy166II GTNNAC 6 cut(s) 731, 1157, 1372, 1374, 1708, 1910
Hpy8I GTNNAC 6 cut(s) 731, 1157, 1372, 1374, 1708, 1910
Hpy99I CGWCG 2 cut(s) 1672, 1974
HpyAV CCTTC 4 cut(s) 1030, 1310, 1387, 1909
HpyCH4III ACNGT 2 cut(s) 1627, 2116
HpyCH4IV ACGT 1 cut(s) 1601
HpyF10VI GCNNNNNNNGC 4 cut(s) 706, 836, 1343, 2027
HpyF3I CTNAG 8 cut(s) 117, 195, 201, 450, 939, 1901, 2043, 2085
HpySE526I ACGT 1 cut(s) 1601
HspAI GCGC 1 cut(s) 2030
Ksp22I TGATCA 1 cut(s) 618
Kzo9I GATC 5 cut(s) 112, 618, 1039, 1531, 1843
LguI GCTCTTC 1 cut(s) 1725
LmnI GCTCC 7 cut(s) 490, 583, 772, 1387, 1504, 1539, 2125
Lsp1109I GCAGC 6 cut(s) 182, 221, 224, 249, 787, 2078
LweI GCATC 2 cut(s) 1075, 1262
MaeI CTAG 6 cut(s) 281, 860, 896, 1149, 1338, 1742
MaeII ACGT 1 cut(s) 1601
MaeIII GTNAC 4 cut(s) 338, 498, 987, 1981
MalI GATC 5 cut(s) 114, 620, 1041, 1533, 1845
MboI GATC 5 cut(s) 112, 618, 1039, 1531, 1843
MflI RGATCY 2 cut(s) 1039, 1531
MhlI GDGCHC 2 cut(s) 138, 580
MlyI GAGTC 3 cut(s) 629, 1276, 2048
MmeI TCCRAC 3 cut(s) 208, 1445, 1952
Mph1103I ATGCAT 1 cut(s) 373
MroXI GAANNNNTTC 4 cut(s) 653, 1085, 1089, 1643
MseI TTAA 7 cut(s) 687, 759, 797, 1491, 1613, 1784, 2073
MslI CAYNNNNRTG 2 cut(s) 1526, 2009
MspA1I CMGCKG 2 cut(s) 1351, 1401
MspCI CTTAAG 1 cut(s) 758
MspR9I CCNGG 2 cut(s) 737, 782
MvaI CCWGG 2 cut(s) 737, 782
MvnI CGCG 2 cut(s) 1236, 2032
MwoI GCNNNNNNNGC 4 cut(s) 706, 836, 1343, 2027
NcoI CCATGG 1 cut(s) 486
NdeII GATC 5 cut(s) 112, 618, 1039, 1531, 1843
NlaIV GGNNCC 7 cut(s) 136, 190, 433, 1041, 1533, 1541, 1664
NmuCI GTSAC 1 cut(s) 987
NsiI ATGCAT 1 cut(s) 373
NspI RCATGY 4 cut(s) 244, 292, 839, 1610
PaeI GCATGC 1 cut(s) 839
PagI TCATGA 1 cut(s) 109
PciSI GCTCTTC 1 cut(s) 1725
PdmI GAANNNNTTC 4 cut(s) 653, 1085, 1089, 1643
PfeI GAWTC 4 cut(s) 512, 1417, 1757, 1808
PfoI TCCNGGA 1 cut(s) 735
PkrI GCNGC 7 cut(s) 11, 172, 211, 214, 239, 777, 2068
PleI GAGTC 3 cut(s) 629, 1276, 2047
PpsI GAGTC 3 cut(s) 629, 1276, 2047
PsiI TTATAA 1 cut(s) 1680
Psp6I CCWGG 2 cut(s) 735, 780
PspFI CCCAGC 2 cut(s) 1347, 1351
PspGI CCWGG 2 cut(s) 735, 780
PspN4I GGNNCC 7 cut(s) 136, 190, 433, 1041, 1533, 1541, 1664
PspOMI GGGCCC 1 cut(s) 134
PspPI GGNCC 4 cut(s) 134, 135, 1166, 1662
PsuI RGATCY 2 cut(s) 1039, 1531
PvuII CAGCTG 1 cut(s) 1351
RsaI GTAC 6 cut(s) 65, 307, 1187, 1201, 1373, 1574
RsaNI GTAC 6 cut(s) 64, 306, 1186, 1200, 1372, 1573
RseI CAYNNNNRTG 2 cut(s) 1526, 2009
SapI GCTCTTC 1 cut(s) 1725
SaqAI TTAA 7 cut(s) 687, 759, 797, 1491, 1613, 1784, 2073
SatI GCNGC 7 cut(s) 10, 171, 210, 213, 238, 776, 2067
Sau3AI GATC 5 cut(s) 112, 618, 1039, 1531, 1843
Sau96I GGNCC 4 cut(s) 134, 135, 1166, 1662
ScaI AGTACT 1 cut(s) 1201
SchI GAGTC 3 cut(s) 629, 1276, 2048
ScrFI CCNGG 2 cut(s) 737, 782
SduI GDGCHC 2 cut(s) 138, 580
SfaNI GCATC 2 cut(s) 1075, 1262
SfcI CTRYAG 2 cut(s) 507, 1910
SinI GGWCC 1 cut(s) 1662
SmiMI CAYNNNNRTG 2 cut(s) 1526, 2009
SmlI CTYRAG 5 cut(s) 80, 758, 904, 1044, 1555
SmoI CTYRAG 5 cut(s) 80, 758, 904, 1044, 1555
SphI GCATGC 1 cut(s) 839
SsiI CCGC 4 cut(s) 828, 1131, 1214, 1401
SspMI CTAG 6 cut(s) 281, 860, 896, 1149, 1338, 1742
StyD4I CCNGG 2 cut(s) 735, 780
StyI CCWWGG 5 cut(s) 486, 895, 977, 1535, 1774
TaaI ACNGT 2 cut(s) 1627, 2116
TaiI ACGT 1 cut(s) 1604
TaqI TCGA 3 cut(s) 657, 1547, 1834
TatI WGTACW 4 cut(s) 63, 305, 1199, 1371
TfiI GAWTC 4 cut(s) 512, 1417, 1757, 1808
Tru1I TTAA 7 cut(s) 687, 759, 797, 1491, 1613, 1784, 2073
Tru9I TTAA 7 cut(s) 687, 759, 797, 1491, 1613, 1784, 2073
TscAI CASTG 6 cut(s) 100, 160, 588, 637, 974, 2011
TseFI GTSAC 1 cut(s) 987
TseI GCWGC 7 cut(s) 9, 170, 209, 212, 237, 775, 2066
Tsp45I GTSAC 1 cut(s) 987
TspGWI ACGGA 3 cut(s) 1005, 1100, 2051
TspRI CASTG 6 cut(s) 100, 160, 588, 637, 974, 2011
Vha464I CTTAAG 1 cut(s) 758
VpaK11BI GGWCC 1 cut(s) 1662
XagI CCTNNNNNAGG 1 cut(s) 2074
XapI RAATTY 4 cut(s) 811, 1085, 1456, 1579
XceI RCATGY 4 cut(s) 244, 292, 839, 1610
XmaJI CCTAGG 1 cut(s) 895
XmiI GTMKAC 2 cut(s) 1156, 1909
XmnI GAANNNNTTC 4 cut(s) 653, 1085, 1089, 1643
XspI CTAG 6 cut(s) 281, 860, 896, 1149, 1338, 1742
ZrmI AGTACT 1 cut(s) 1201
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.