RchiOBHm_Chr5g0073071

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000183
Physical Location & Seq
Reverse (-)
79132888 .. 79140841
7954 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34798

Sequence Viewer

Length: 3027 bp
ATGGGTTGCTGCACTTTCTCGTCGGACGATATTGTAATAAAGTCTCCAAATGACAAGCGACTGTACAGAGTGATTAAGCTTGAGAATGGCCTCACTGCATTGCTCATTCACGATCCTCAGGTTTACCCACAAGGACCACCCGACCATTCCTCCGACGTTGAACAACGAGAAGAAGACAGTGAGGCTGTAGTAGAAGGAGAGGAAGGTGAAGTTAAAACTAAGGAAGAGGGAGGTGCTTCTCAGACTAAGAAGGCAGCAGCAGCAATGTGTGTCGGAATAGGCAGCTTCTCTGACCCTCTCGAGGCTCAGGGGCTTGCACACTTTCTAGAACACATGCTCTTCATGGGGAGTACTAAGTTCCCAGATGAAAATGAGTATGCTAGTTACTTGTCCAAGCATGGAGGGTCGTCAAATGCACACACAGAAACAGAACATACTTGCTACTACTTTGATGTGAAACGAGAATTTCTTAAGGGTGCCTTGAAAAGATTTTCTCAGTTCTTTGTTTCACCTCTAATGAAAAGTGAAGCCATGGCAAGAGAGGTACAGGCTATAGATTCAGAGTTTAACACGGTTCTGCAAAACGATTTTTGCCGCCTTCAACAACTTCAAGGCCATACATCCTCACCTGGTCACCCATTTAATAAATTTTCTTGGGGAAATAAGAAGAGCTTGGATGATGCAAAGAAAAAAGGGATCAACTTGCAGGAACAAATACTTAAATTGTACAGAGACAATTACCACGGTGGATTAATGAAGCTAGTTGTCATTGGTGGAGGTAAAAGTTATAATCTCTCGATGTACTTGAAAGATCCCCAAGTAAATCTGGAGTTCAAGGCAGAAGGTCCTATTTGGAAAGCTGGAAAACTTTACAGGCTAGAGGCTGTTGACGATGTTCATATACTCCACCTAGCATGGACACTTCCATGTCTTCAAGAACACTATTTGAAGAAACCAGAAGATTACTTGAGTCATCTGCTTGGGCATGAGGGCAGGGGAAGTTTGCATTTCTACCTGAAAGCTAGAGGGTGGGTAACATCTTTAGATACTTGTTTGAGCGGGATGGACTGCTCTTCTGTGGCTTATATCTTTTGCATGGTCATATACCTCACTGACTCTGGATTGGAGAAGATGTTTGAAATAATTGGATTTGTGTATCAATACATTAAGTTATTGCGTCAAATGTCACCACAAGAGTGGATATTTCGGGAACTTCAGGATATTGGGAACATGGACTTTAGATTTGCAGAGGAGCAGGACCAGGATGATTCTGCTTCACAACTTGCAGAAAATTTACTATATTATGCAACAGAGCATGTTATTTATGGGGACTATGTGAACGAGAGTTGGGATAAGGAATTGATAGAATATGTTCTTGGTTTCTTCAGACCAGAAAACATGAGGATTGATGTGATATCAAAGTCCTTATTTAAGTCAGAAGATTTCCAGTGTGAGCCATGGTTTGGATCACATTATACTGAGGAAGATATATCTCCATCTTTAATAGATTTATGGAAGAATCCTCAAGAAATTAATGTTTCATTGCATCTGCCGGCAAAGAATGATTTCATTCCTCGTGATTTCTCCATCCGTTCTGATGTTTTGTGCATTGATACTGCAACGACATCTTATCCAAAATGTATACTTGATGAACCATTGATGAAGTTTTGGTACAAGCTCGACAGTACATTTAAACTTCCACATGTAAATACATACTTCAGCATCAATCTGAAGGGTGCATATGATGATGTGAAGAGTTGTGTTTTGACTAGCTTATACATTGACCTTCTCACAGATCAGCTGAATGAGATCCTCTATGAGCTCAGTGTCTCATCTCTTATAACTAGTCATTTTGCAGTTCAGATTAGATGGAAACTATTTGTATTTAAATGGTTTCAGTGGGAGCACTACTTGCATTTGATAGTAGAGACTAGAAACTGTTGCGCCTGTGTTGCCAGCCTGGGAACTTCTGTTTCTTTGTCTCTGGACAAGCTGCAGTTAGAGGTATACGGTTTCAATGATAAGCTTCCAGCTCTGTTATCAAAAATTTTGGAAACAATAAAAAGTTTCTTGCCAACTGATGATCGTTTTGAGGTTTTTAAAGAAGACATGGAGCGAGCATATACGAACGCCAATATGGATCCTTGGAGTTACTCAACATACTTGAGAGATCAAGTTCTGTTGCAGAAATTCTATACCGTAGATGAGCAGTTGCATGTTTTGAAGGGATTGTCTGTTTCTGATCTGAAGTCTTTCATTCCTGAGATTTTTTCCCAGTTGTATTTTCAAATTGAGCAGGCAGTGAAGAGTGAGTCCGTCAGACTAAAAGTATTGATTGACCTTTTTCAGGAAATTGTACAGGAACCACTTTTTAATCAACTAAGGACGAAGGAGCAGCTTGGTTATGTTGTTCTCTGTGGCCAGAATTGTACGTGCAATGTTTTTGGCTTCTATTTCTGTGTTCAGTCATCCGAGTACAACCCGATCCACCTTCAAGGCAGACTGGACAACTTTATAGATGGTCTGGAAGAGTTGTTGGAAGGGCTGGACGATGATTCCTTTGAGAATTATAAAGGTGGACTAATGGCCAAGGTTTTGGAGAAAGATGCATACCTCACATGTGAAACCAATCGATTGTGGACTCAGATTCTTGATAAATGGTATACGTTTGACTGTTCGAAAAAGGCCGCAGAACAACTCAGAAGCATTCAGAAGGAGGATGTTATCAACTTTTACAAGACCTATTTGCAACAATCATCTCCAAAGCGTCGAAGACTTGCAACTCGTGTATGGGGTTGCAATCAGCGTTTTAAGAGGCGACTGCATGAGGCGAGGCGTTTTGCCCGAGCATTAAGCCTCAAAAGCCTTAAGGCGCTTTTAATGTGCAAGTCATTGAAGACCTTGCAAGAAGCTGAAGAAGCACGACCGGAGTCTGTGCAAGTCATTGAAGACCTTGCAGCCTTTAAGATGTCGTCCAAGTTTTATGGTTGTAAAAGATGTCAAAAAGTTACTCCTCTGAAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

1008

Amino Acids

116.39

Weight (kDa)

5.57

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 81 - 210 2.8e-33 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 237 - 406 2.8e-09 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 413 - 613 1.1e-58 Middle or third domain of peptidase_M16
Peptidase_M16_M PF16187 648 - 739 8.9e-20 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 760 - 859 4.6e-11 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 781 - 880 7.2e-23 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 789, 1841, 2571
AccB1I GGYRCC 1 cut(s) 476
AccB7I CCANNNNNTGG 1 cut(s) 1463
AccBSI CCGCTC 1 cut(s) 1059
AccI GTMKAC 3 cut(s) 1642, 2007, 2663
AciI CCGC 3 cut(s) 595, 1059, 2688
AclWI GGATC 8 cut(s) 107, 704, 806, 1474, 1804, 2135, 2148, 2479
AcoI YGGCCR 2 cut(s) 2419, 2586
AcsI RAATTY 5 cut(s) 464, 647, 1291, 2046, 2189
AcuI CTGAAG 6 cut(s) 1199, 1369, 1702, 1751, 2267, 2934
AfiI CCNNNNNNNGG 3 cut(s) 301, 1463, 2347
AflII CTTAAG 2 cut(s) 470, 2867
AflIII ACRYGT 2 cut(s) 1702, 2618
AhlI ACTAGT 1 cut(s) 1844
AjnI CCWGG 3 cut(s) 628, 1260, 1959
AjuI GAANNNNNNNTTGG 2 cut(s) 1359, 1391
AleI CACNNNNGTG 1 cut(s) 1195
Alw21I GWGCWC 2 cut(s) 1824, 1908
Alw26I GTCTC 5 cut(s) 48, 726, 1834, 1922, 1986
AlwI GGATC 8 cut(s) 107, 704, 806, 1474, 1804, 2135, 2148, 2479
Ama87I CYCGRG 2 cut(s) 299, 2844
AoxI GGCC 5 cut(s) 88, 613, 2419, 2586, 2685
ApeKI GCWGC 8 cut(s) 9, 254, 257, 260, 282, 1993, 2395, 2957
ApoI RAATTY 5 cut(s) 464, 647, 1291, 2046, 2189
ArsI GACNNNNNNTTYG 2 cut(s) 1226, 1258
AseI ATTAAT 2 cut(s) 752, 1533
Asp700I GAANNNNTTC 3 cut(s) 1371, 1565, 2252
AspLEI GCGC 2 cut(s) 1946, 2875
AspS9I GGNCC 3 cut(s) 134, 845, 1258
AsuHPI GGTGA 5 cut(s) 218, 501, 618, 626, 1179
AsuII TTCGAA 1 cut(s) 2678
AvaI CYCGRG 2 cut(s) 299, 2844
AvaII GGWCC 3 cut(s) 134, 845, 1258
AxyI CCTNAGG 1 cut(s) 117
BalI TGGCCA 2 cut(s) 2421, 2588
BamHI GGATCC 1 cut(s) 2140
BanI GGYRCC 1 cut(s) 476
BanII GRGCYC 1 cut(s) 1824
BarI GAAGNNNNNNTAC 2 cut(s) 1655, 1687
BauI CACGAG 2 cut(s) 1575, 2784
BbsI GAAGAC 6 cut(s) 180, 923, 2112, 2779, 2903, 2955
Bbv12I GWGCWC 2 cut(s) 1824, 1908
BbvI GCAGC 7 cut(s) 266, 269, 272, 294, 1980, 2407, 2969
BccI CCATC 5 cut(s) 1057, 1504, 1595, 1863, 2513
BciT130I CCWGG 3 cut(s) 630, 1262, 1961
BcoDI GTCTC 5 cut(s) 48, 726, 1834, 1922, 1986
BcuI ACTAGT 1 cut(s) 1844
BfaI CTAG 9 cut(s) 326, 381, 761, 878, 911, 1023, 1770, 1845, 1932
BfmI CTRYAG 3 cut(s) 186, 552, 1994
BfoI RGCGCY 1 cut(s) 2876
BfrI CTTAAG 2 cut(s) 470, 2867
BmcAI AGTACT 1 cut(s) 352
Bme1390I CCNGG 3 cut(s) 630, 1262, 1961
Bme18I GGWCC 3 cut(s) 134, 845, 1258
BmeT110I CYCGRG 2 cut(s) 299, 2844
BmgT120I GGNCC 3 cut(s) 134, 845, 1258
BmiI GGNNCC 3 cut(s) 478, 2142, 2364
BmrFI CCNGG 3 cut(s) 630, 1262, 1961
BmrI ACTGGG 1 cut(s) 2269
BmsI GCATC 4 cut(s) 670, 1555, 1731, 2596
BmuI ACTGGG 1 cut(s) 2269
BoxI GACNNNNGTC 1 cut(s) 2929
BpiI GAAGAC 6 cut(s) 180, 923, 2112, 2779, 2903, 2955
BpmI CTGGAG 1 cut(s) 848
Bpu10I CCTNAGC 1 cut(s) 306
Bpu14I TTCGAA 1 cut(s) 2678
BpuEI CTTGAG 4 cut(s) 101, 988, 1509, 2185
Bsa29I ATCGAT 1 cut(s) 2632
BsaAI YACGTR 1 cut(s) 2433
BsaJI CCNNGG 6 cut(s) 531, 742, 1457, 1960, 2144, 2589
BsaWI WCCGGW 1 cut(s) 2926
BsaXI ACNNNNNCTCC 4 cut(s) 134, 164, 1118, 1148
Bsc4I CCNNNNNNNGG 3 cut(s) 301, 1463, 2347
Bse118I RCCGGY 1 cut(s) 1552
Bse1I ACTGG 3 cut(s) 1447, 2275, 2508
Bse21I CCTNAGG 1 cut(s) 117
Bse3DI GCAATG 4 cut(s) 98, 270, 1541, 2443
BseBI CCWGG 3 cut(s) 630, 1262, 1961
BseCI ATCGAT 1 cut(s) 2632
BseDI CCNNGG 6 cut(s) 531, 742, 1457, 1960, 2144, 2589
BseGI GGATG 7 cut(s) 620, 682, 1068, 1270, 1587, 2468, 2725
BseLI CCNNNNNNNGG 3 cut(s) 301, 1463, 2347
BseMI GCAATG 4 cut(s) 98, 270, 1541, 2443
BseMII CTCAG 9 cut(s) 131, 254, 320, 509, 1470, 1837, 2253, 2657, 2713
BseNI ACTGG 3 cut(s) 1447, 2275, 2508
BseRI GAGGAG 2 cut(s) 1265, 3003
BseXI GCAGC 7 cut(s) 266, 269, 272, 294, 1980, 2407, 2969
Bsh1285I CGRYCG 1 cut(s) 2927
BshFI GGCC 5 cut(s) 90, 615, 2421, 2588, 2687
BshNI GGYRCC 1 cut(s) 476
BshVI ATCGAT 1 cut(s) 2632
BsiEI CGRYCG 1 cut(s) 2927
BsiHKAI GWGCWC 2 cut(s) 1824, 1908
BsiHKCI CYCGRG 2 cut(s) 299, 2844
BsiSI CCGG 2 cut(s) 1553, 2927
BslFI GGGAC 1 cut(s) 1343
BslI CCNNNNNNNGG 3 cut(s) 301, 1463, 2347
BsmAI GTCTC 5 cut(s) 48, 726, 1834, 1922, 1986
BsmFI GGGAC 1 cut(s) 1343
BsmI GAATGC 1 cut(s) 2706
BsnI GGCC 5 cut(s) 90, 615, 2421, 2588, 2687
BsoBI CYCGRG 2 cut(s) 299, 2844
Bsp119I TTCGAA 1 cut(s) 2678
Bsp1286I GDGCHC 2 cut(s) 1824, 1908
Bsp1407I TGTACA 3 cut(s) 63, 726, 2356
Bsp19I CCATGG 2 cut(s) 531, 1457
BspACI CCGC 3 cut(s) 595, 1059, 2688
BspANI GGCC 5 cut(s) 90, 615, 2421, 2588, 2687
BspCNI CTCAG 9 cut(s) 130, 253, 319, 508, 1471, 1836, 2254, 2656, 2712
BspDI ATCGAT 1 cut(s) 2632
BspLI GGNNCC 3 cut(s) 478, 2142, 2364
BspMAI CTGCAG 1 cut(s) 1998
BspPI GGATC 8 cut(s) 107, 704, 806, 1474, 1804, 2135, 2148, 2479
BspQI GCTCTTC 3 cut(s) 344, 662, 1078
BspT104I TTCGAA 1 cut(s) 2678
BspT107I GGYRCC 1 cut(s) 476
BspTI CTTAAG 2 cut(s) 470, 2867
BsrBI CCGCTC 1 cut(s) 1059
BsrDI GCAATG 4 cut(s) 98, 270, 1541, 2443
BsrFI RCCGGY 1 cut(s) 1552
BsrGI TGTACA 3 cut(s) 63, 726, 2356
BsrI ACTGG 3 cut(s) 1447, 2275, 2508
BssAI RCCGGY 1 cut(s) 1552
BssECI CCNNGG 6 cut(s) 531, 742, 1457, 1960, 2144, 2589
BssNAI GTATAC 3 cut(s) 1643, 2008, 2664
BssSI CACGAG 2 cut(s) 1575, 2784
BssT1I CCWWGG 4 cut(s) 531, 1457, 2144, 2589
Bst1107I GTATAC 3 cut(s) 1643, 2008, 2664
Bst2BI CACGAG 2 cut(s) 1575, 2784
Bst2UI CCWGG 3 cut(s) 630, 1262, 1961
Bst4CI ACNGT 9 cut(s) 63, 179, 574, 746, 1685, 1940, 2012, 2200, 2675
Bst6I CTCTTC 7 cut(s) 219, 344, 662, 1078, 1748, 2300, 2523
BstAFI CTTAAG 2 cut(s) 470, 2867
BstAPI GCANNNNNTGC 1 cut(s) 1912
BstAUI TGTACA 3 cut(s) 63, 726, 2356
BstBAI YACGTR 1 cut(s) 2433
BstBI TTCGAA 1 cut(s) 2678
BstC8I GCNNGC 5 cut(s) 315, 1554, 1957, 2118, 2298
BstDSI CCRYGG 3 cut(s) 531, 742, 1457
BstEII GGTNACC 1 cut(s) 632
BstENI CCTNNNNNAGG 1 cut(s) 2345
BstF5I GGATG 7 cut(s) 620, 682, 1068, 1270, 1587, 2468, 2725
BstH2I RGCGCY 1 cut(s) 2876
BstHHI GCGC 2 cut(s) 1946, 2875
BstMAI GTCTC 5 cut(s) 48, 726, 1834, 1922, 1986
BstMCI CGRYCG 1 cut(s) 2927
BstMWI GCNNNNNNNGC 5 cut(s) 260, 1912, 1952, 2862, 2918
BstNI CCWGG 3 cut(s) 630, 1262, 1961
BstNSI RCATGY 5 cut(s) 337, 1319, 1706, 2219, 2622
BstPAI GACNNNNGTC 1 cut(s) 2929
BstPI GGTNACC 1 cut(s) 632
BstSCI CCNGG 3 cut(s) 628, 1260, 1959
BstSFI CTRYAG 3 cut(s) 186, 552, 1994
BstV1I GCAGC 7 cut(s) 266, 269, 272, 294, 1980, 2407, 2969
BstV2I GAAGAC 6 cut(s) 180, 923, 2112, 2779, 2903, 2955
BstX2I RGATCY 3 cut(s) 811, 1809, 2140
BstXI CCANNNNNNTGG 2 cut(s) 1197, 2596
BstYI RGATCY 3 cut(s) 811, 1809, 2140
BstZ17I GTATAC 3 cut(s) 1643, 2008, 2664
Bsu15I ATCGAT 1 cut(s) 2632
Bsu36I CCTNAGG 1 cut(s) 117
BsuRI GGCC 5 cut(s) 90, 615, 2421, 2588, 2687
BsuTUI ATCGAT 1 cut(s) 2632
BtgI CCRYGG 3 cut(s) 531, 742, 1457
BtsCI GGATG 7 cut(s) 620, 682, 1068, 1270, 1587, 2468, 2725
BtsI GCAGTG 2 cut(s) 93, 2307
BtsIMutI CAGTG 7 cut(s) 93, 184, 1110, 1454, 1831, 1904, 2307
Cac8I GCNNGC 5 cut(s) 315, 1554, 1957, 2118, 2298
CfoI GCGC 2 cut(s) 1946, 2875
Cfr10I RCCGGY 1 cut(s) 1552
Cfr13I GGNCC 3 cut(s) 134, 845, 1258
ClaI ATCGAT 1 cut(s) 2632
CseI GACGC 2 cut(s) 1166, 2756
CsiI ACCWGGT 1 cut(s) 628
DraI TTTAAA 3 cut(s) 1693, 1888, 2101
EaeI YGGCCR 2 cut(s) 2419, 2586
Eam1104I CTCTTC 7 cut(s) 219, 344, 662, 1078, 1748, 2300, 2523
EarI CTCTTC 7 cut(s) 219, 344, 662, 1078, 1748, 2300, 2523
Ecl136II GAGCTC 1 cut(s) 1822
Eco130I CCWWGG 4 cut(s) 531, 1457, 2144, 2589
Eco24I GRGCYC 1 cut(s) 1824
Eco32I GATATC 1 cut(s) 1416
Eco47I GGWCC 3 cut(s) 134, 845, 1258
Eco53kI GAGCTC 1 cut(s) 1822
Eco57I CTGAAG 6 cut(s) 1199, 1369, 1702, 1751, 2267, 2934
Eco81I CCTNAGG 1 cut(s) 117
Eco88I CYCGRG 2 cut(s) 299, 2844
Eco91I GGTNACC 1 cut(s) 632
EcoICRI GAGCTC 1 cut(s) 1822
EcoNI CCTNNNNNAGG 1 cut(s) 2345
EcoO109I RGGNCCY 1 cut(s) 845
EcoO65I GGTNACC 1 cut(s) 632
EcoRII CCWGG 3 cut(s) 628, 1260, 1959
EcoRV GATATC 1 cut(s) 1416
EcoT14I CCWWGG 4 cut(s) 531, 1457, 2144, 2589
EcoT22I ATGCAT 1 cut(s) 2611
EcoT38I GRGCYC 1 cut(s) 1824
ErhI CCWWGG 4 cut(s) 531, 1457, 2144, 2589
FalI AAGNNNNNCTT 4 cut(s) 464, 496, 656, 688
FaqI GGGAC 1 cut(s) 1343
FauI CCCGC 1 cut(s) 1052
FauNDI CATATG 1 cut(s) 1741
FblI GTMKAC 3 cut(s) 1642, 2007, 2663
FokI GGATG 7 cut(s) 607, 689, 1075, 1277, 1574, 2455, 2732
FriOI GRGCYC 1 cut(s) 1824
FspBI CTAG 9 cut(s) 326, 381, 761, 878, 911, 1023, 1770, 1845, 1932
GlaI GCGC 2 cut(s) 1945, 2874
GsuI CTGGAG 1 cut(s) 848
HaeII RGCGCY 1 cut(s) 2876
HaeIII GGCC 5 cut(s) 90, 615, 2421, 2588, 2687
HapII CCGG 2 cut(s) 1553, 2927
HgaI GACGC 2 cut(s) 1166, 2756
HhaI GCGC 2 cut(s) 1946, 2875
Hin6I GCGC 2 cut(s) 1944, 2873
HinP1I GCGC 2 cut(s) 1944, 2873
HincII GTYRAC 1 cut(s) 889
HindII GTYRAC 1 cut(s) 889
HindIII AAGCTT 2 cut(s) 77, 2024
HpaII CCGG 2 cut(s) 1553, 2927
HphI GGTGA 5 cut(s) 218, 501, 618, 626, 1179
Hpy166II GTNNAC 8 cut(s) 124, 889, 1339, 1643, 2008, 2579, 2640, 2664
Hpy8I GTNNAC 8 cut(s) 124, 889, 1339, 1643, 2008, 2579, 2640, 2664
Hpy99I CGWCG 3 cut(s) 25, 158, 2772
HpyCH4III ACNGT 9 cut(s) 63, 179, 574, 746, 1685, 1940, 2012, 2200, 2675
HpyCH4IV ACGT 3 cut(s) 156, 2432, 2666
HpyF10VI GCNNNNNNNGC 5 cut(s) 260, 1912, 1952, 2862, 2918
HpySE526I ACGT 3 cut(s) 156, 2432, 2666
HspAI GCGC 2 cut(s) 1944, 2873
KroI GCCGGC 1 cut(s) 1552
KroNI GCCGGC 1 cut(s) 1554
LguI GCTCTTC 3 cut(s) 344, 662, 1078
LmnI GCTCC 4 cut(s) 1252, 1903, 2113, 2392
Lsp1109I GCAGC 7 cut(s) 266, 269, 272, 294, 1980, 2407, 2969
LweI GCATC 4 cut(s) 670, 1555, 1731, 2596
MabI ACCWGGT 1 cut(s) 628
MaeI CTAG 9 cut(s) 326, 381, 761, 878, 911, 1023, 1770, 1845, 1932
MaeII ACGT 3 cut(s) 156, 2432, 2666
MaeIII GTNAC 6 cut(s) 383, 632, 1033, 1185, 2150, 3007
MbiI CCGCTC 1 cut(s) 1059
MflI RGATCY 3 cut(s) 811, 1809, 2140
MhlI GDGCHC 2 cut(s) 1824, 1908
MlsI TGGCCA 2 cut(s) 2421, 2588
MluNI TGGCCA 2 cut(s) 2421, 2588
MlyI GAGTC 5 cut(s) 979, 1109, 2321, 2635, 2939
MmeI TCCRAC 3 cut(s) 177, 253, 2517
Mox20I TGGCCA 2 cut(s) 2421, 2588
Mph1103I ATGCAT 1 cut(s) 2611
MroNI GCCGGC 1 cut(s) 1552
MroXI GAANNNNTTC 3 cut(s) 1371, 1565, 2252
MscI TGGCCA 2 cut(s) 2421, 2588
MslI CAYNNNNRTG 2 cut(s) 925, 1195
Msp20I TGGCCA 2 cut(s) 2421, 2588
MspA1I CMGCKG 1 cut(s) 1801
MspCI CTTAAG 2 cut(s) 470, 2867
MspI CCGG 2 cut(s) 1553, 2927
MspR9I CCNGG 3 cut(s) 630, 1262, 1961
Mva1269I GAATGC 1 cut(s) 2706
MvaI CCWGG 3 cut(s) 630, 1262, 1961
MwoI GCNNNNNNNGC 5 cut(s) 260, 1912, 1952, 2862, 2918
NaeI GCCGGC 1 cut(s) 1554
NcoI CCATGG 2 cut(s) 531, 1457
NdeI CATATG 1 cut(s) 1741
NgoMIV GCCGGC 1 cut(s) 1552
NlaIV GGNNCC 3 cut(s) 478, 2142, 2364
NmuCI GTSAC 2 cut(s) 632, 1185
NsiI ATGCAT 1 cut(s) 2611
NspI RCATGY 5 cut(s) 337, 1319, 1706, 2219, 2622
NspV TTCGAA 1 cut(s) 2678
OliI CACNNNNGTG 1 cut(s) 1195
PaeR7I CTCGAG 1 cut(s) 299
PciI ACATGT 2 cut(s) 1702, 2618
PciSI GCTCTTC 3 cut(s) 344, 662, 1078
PctI GAATGC 1 cut(s) 2706
PdiI GCCGGC 1 cut(s) 1554
PdmI GAANNNNTTC 3 cut(s) 1371, 1565, 2252
PfeI GAWTC 5 cut(s) 557, 1268, 1519, 2555, 2647
PflMI CCANNNNNTGG 1 cut(s) 1463
PleI GAGTC 5 cut(s) 978, 1109, 2320, 2635, 2938
PpsI GAGTC 5 cut(s) 978, 1109, 2320, 2635, 2938
Ppu21I YACGTR 1 cut(s) 2433
PpuMI RGGWCCY 1 cut(s) 845
PscI ACATGT 2 cut(s) 1702, 2618
PshAI GACNNNNGTC 1 cut(s) 2929
PshBI ATTAAT 2 cut(s) 752, 1533
PsiI TTATAA 3 cut(s) 789, 1841, 2571
Psp124BI GAGCTC 1 cut(s) 1824
Psp5II RGGWCCY 1 cut(s) 845
Psp6I CCWGG 3 cut(s) 628, 1260, 1959
PspEI GGTNACC 1 cut(s) 632
PspGI CCWGG 3 cut(s) 628, 1260, 1959
PspN4I GGNNCC 3 cut(s) 478, 2142, 2364
PspPI GGNCC 3 cut(s) 134, 845, 1258
PspPPI RGGWCCY 1 cut(s) 845
PstI CTGCAG 1 cut(s) 1998
PsuI RGATCY 3 cut(s) 811, 1809, 2140
PvuII CAGCTG 1 cut(s) 1801
RseI CAYNNNNRTG 2 cut(s) 925, 1195
SacI GAGCTC 1 cut(s) 1824
SapI GCTCTTC 3 cut(s) 344, 662, 1078
Sau96I GGNCC 3 cut(s) 134, 845, 1258
ScaI AGTACT 1 cut(s) 352
SchI GAGTC 5 cut(s) 979, 1109, 2321, 2635, 2939
ScrFI CCNGG 3 cut(s) 630, 1262, 1961
SduI GDGCHC 2 cut(s) 1824, 1908
SexAI ACCWGGT 1 cut(s) 628
SfaNI GCATC 4 cut(s) 670, 1555, 1731, 2596
SfcI CTRYAG 3 cut(s) 186, 552, 1994
Sfr274I CTCGAG 1 cut(s) 299
SfuI TTCGAA 1 cut(s) 2678
SinI GGWCC 3 cut(s) 134, 845, 1258
SlaI CTCGAG 1 cut(s) 299
SmiI ATTTAAAT 1 cut(s) 1888
SmiMI CAYNNNNRTG 2 cut(s) 925, 1195
SmlI CTYRAG 7 cut(s) 80, 299, 470, 967, 1524, 2164, 2867
SmoI CTYRAG 7 cut(s) 80, 299, 470, 967, 1524, 2164, 2867
SpeI ACTAGT 1 cut(s) 1844
SsiI CCGC 3 cut(s) 595, 1059, 2688
SspMI CTAG 9 cut(s) 326, 381, 761, 878, 911, 1023, 1770, 1845, 1932
SstI GAGCTC 1 cut(s) 1824
StyD4I CCNGG 3 cut(s) 628, 1260, 1959
StyI CCWWGG 4 cut(s) 531, 1457, 2144, 2589
SwaI ATTTAAAT 1 cut(s) 1888
TaaI ACNGT 9 cut(s) 63, 179, 574, 746, 1685, 1940, 2012, 2200, 2675
TaiI ACGT 3 cut(s) 159, 2435, 2669
TaqI TCGA 6 cut(s) 300, 797, 1680, 2632, 2678, 2770
TatI WGTACW 7 cut(s) 63, 350, 726, 801, 1685, 2356, 2475
TauI GCSGC 2 cut(s) 597, 2690
TfiI GAWTC 5 cut(s) 557, 1268, 1519, 2555, 2647
TscAI CASTG 7 cut(s) 100, 184, 1117, 1454, 1831, 1904, 2307
TseFI GTSAC 2 cut(s) 632, 1185
TseI GCWGC 8 cut(s) 9, 254, 257, 260, 282, 1993, 2395, 2957
Tsp45I GTSAC 2 cut(s) 632, 1185
TspGWI ACGGA 2 cut(s) 1580, 2305
TspRI CASTG 7 cut(s) 100, 184, 1117, 1454, 1831, 1904, 2307
Van91I CCANNNNNTGG 1 cut(s) 1463
Vha464I CTTAAG 2 cut(s) 470, 2867
VpaK11BI GGWCC 3 cut(s) 134, 845, 1258
VspI ATTAAT 2 cut(s) 752, 1533
XagI CCTNNNNNAGG 1 cut(s) 2345
XapI RAATTY 5 cut(s) 464, 647, 1291, 2046, 2189
XbaI TCTAGA 1 cut(s) 325
XceI RCATGY 5 cut(s) 337, 1319, 1706, 2219, 2622
XhoI CTCGAG 1 cut(s) 299
XmiI GTMKAC 3 cut(s) 1642, 2007, 2663
XmnI GAANNNNTTC 3 cut(s) 1371, 1565, 2252
XspI CTAG 9 cut(s) 326, 381, 761, 878, 911, 1023, 1770, 1845, 1932
ZrmI AGTACT 1 cut(s) 352
Zsp2I ATGCAT 1 cut(s) 2611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.