Rh5DG520000

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000183
Physical Location & Seq
Reverse (-)
80733291 .. 80740787
7497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG520000.1

Sequence Viewer

Length: 471 bp
ATGCTTAATCAAAGCGTAGTTAAATTTATGATGTGCAGACTTAACTTATACATGTATGTTGTGCGTGCAGAACACATGCTCTTTATGGGGAGTACAAAATTTCCAGATGAGAAGGAGTATGACAGTTACTTGTCCAAGCACGGAGGGTGTTTAAATGCATACACGGAAGTAGAGCATACTTGCTACCATTTTGAAATGAAACGAGAGTTTCTCAAGGGTGCCTTGACAAGATTTTCTCAGTTCTTTGTTTCACCCCTTGTAAAAATTGAAGCCATGGAGCGGGAGTTACAGGCTGTAGATTCAGAAACAAAGCTTGTACAAAACAAAGCTGAGAGCAGAAACTGGAAGCAATTGTTGAATGTGAAGATGATGCTCAGCCGCAGCACAAGAGGAAGGCTTTATGACAATTCTCGGTTTTCTGCATTCGTAGTTTCTTTGCCATATGTAGCAGTACGTGGTGCACCTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

156

Amino Acids

18.28

Weight (kDa)

9.13

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 24 - 117 7e-20 Insulinase (Peptidase family M16)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 218
AccBSI CCGCTC 1 cut(s) 280
AciI CCGC 2 cut(s) 280, 379
AcsI RAATTY 2 cut(s) 23, 98
AfaI GTAC 3 cut(s) 94, 318, 453
AfiI CCNNNNNNNGG 1 cut(s) 279
AflIII ACRYGT 1 cut(s) 51
AgsI TTSAA 3 cut(s) 194, 269, 358
AluBI AGCT 2 cut(s) 313, 329
AluI AGCT 2 cut(s) 313, 329
Alw21I GWGCWC 1 cut(s) 463
Alw44I GTGCAC 1 cut(s) 459
AlwNI CAGNNNCTG 1 cut(s) 342
ApaLI GTGCAC 1 cut(s) 459
ApeKI GCWGC 1 cut(s) 381
ApoI RAATTY 2 cut(s) 23, 98
AsuHPI GGTGA 1 cut(s) 243
BaeGI GKGCMC 1 cut(s) 463
BanI GGYRCC 1 cut(s) 218
Bbv12I GWGCWC 1 cut(s) 463
BbvI GCAGC 1 cut(s) 393
BfmI CTRYAG 1 cut(s) 294
BisI GCNGC 2 cut(s) 379, 382
BlpI GCTNAGC 1 cut(s) 374
BlsI GCNGC 2 cut(s) 380, 383
BmiI GGNNCC 1 cut(s) 220
BmsI GCATC 1 cut(s) 360
BplI GAGNNNNNCTC 2 cut(s) 195, 227
Bpu1102I GCTNAGC 1 cut(s) 374
BpuEI CTTGAG 1 cut(s) 197
BsaAI YACGTR 1 cut(s) 455
BsaJI CCNNGG 1 cut(s) 273
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 1 cut(s) 279
Bse1I ACTGG 1 cut(s) 347
BseDI CCNNGG 1 cut(s) 273
BseLI CCNNNNNNNGG 1 cut(s) 279
BseMII CTCAG 3 cut(s) 251, 321, 388
BseNI ACTGG 1 cut(s) 347
BseSI GKGCMC 1 cut(s) 463
BseXI GCAGC 1 cut(s) 393
BsgI GTGCAG 2 cut(s) 55, 87
BshNI GGYRCC 1 cut(s) 218
BsiHKAI GWGCWC 1 cut(s) 463
BslI CCNNNNNNNGG 1 cut(s) 279
BsmI GAATGC 1 cut(s) 422
Bsp1286I GDGCHC 1 cut(s) 463
Bsp1407I TGTACA 1 cut(s) 316
Bsp1720I GCTNAGC 1 cut(s) 374
Bsp19I CCATGG 1 cut(s) 273
BspACI CCGC 2 cut(s) 280, 379
BspCNI CTCAG 3 cut(s) 250, 322, 387
BspLI GGNNCC 1 cut(s) 220
BspT107I GGYRCC 1 cut(s) 218
BsrBI CCGCTC 1 cut(s) 280
BsrGI TGTACA 1 cut(s) 316
BsrI ACTGG 1 cut(s) 347
BssECI CCNNGG 1 cut(s) 273
BssT1I CCWWGG 1 cut(s) 273
Bst4CI ACNGT 1 cut(s) 125
BstAUI TGTACA 1 cut(s) 316
BstBAI YACGTR 1 cut(s) 455
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 3 cut(s) 237, 330, 374
BstDSI CCRYGG 1 cut(s) 273
BstNSI RCATGY 2 cut(s) 55, 79
BstSFI CTRYAG 1 cut(s) 294
BstSLI GKGCMC 1 cut(s) 463
BstV1I GCAGC 1 cut(s) 393
BtgI CCRYGG 1 cut(s) 273
Cac8I GCNNGC 1 cut(s) 66
CaiI CAGNNNCTG 1 cut(s) 342
Csp6I GTAC 3 cut(s) 93, 317, 452
CviAII CATG 3 cut(s) 52, 76, 274
CviJI RGCY 6 cut(s) 272, 293, 313, 329, 378, 397
CviKI_1 RGCY 6 cut(s) 272, 293, 313, 329, 378, 397
CviQI GTAC 3 cut(s) 93, 317, 452
DdeI CTNAG 3 cut(s) 237, 330, 374
DraI TTTAAA 1 cut(s) 153
Eco130I CCWWGG 1 cut(s) 273
EcoT14I CCWWGG 1 cut(s) 273
EcoT22I ATGCAT 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 3 cut(s) 55, 79, 277
FalI AAGNNNNNCTT 2 cut(s) 206, 238
FatI CATG 3 cut(s) 51, 75, 273
FauI CCCGC 1 cut(s) 273
FauNDI CATATG 1 cut(s) 442
Fnu4HI GCNGC 2 cut(s) 379, 382
Fsp4HI GCNGC 2 cut(s) 379, 382
GluI GCNGC 2 cut(s) 379, 382
Hin1II CATG 3 cut(s) 55, 79, 277
HindIII AAGCTT 1 cut(s) 311
HinfI GANTC 1 cut(s) 299
HphI GGTGA 1 cut(s) 243
Hpy166II GTNNAC 1 cut(s) 461
Hpy188I TCNGA 1 cut(s) 304
Hpy188III TCNNGA 1 cut(s) 104
Hpy8I GTNNAC 1 cut(s) 461
HpyAV CCTTC 2 cut(s) 106, 387
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4IV ACGT 1 cut(s) 454
HpyCH4V TGCA 5 cut(s) 36, 68, 158, 422, 461
HpyF3I CTNAG 3 cut(s) 237, 330, 374
HpySE526I ACGT 1 cut(s) 454
Hsp92II CATG 3 cut(s) 55, 79, 277
LmnI GCTCC 1 cut(s) 277
LpnPI CCDG 3 cut(s) 117, 275, 328
Lsp1109I GCAGC 1 cut(s) 393
LweI GCATC 1 cut(s) 360
MaeII ACGT 1 cut(s) 454
MaeIII GTNAC 2 cut(s) 125, 285
MbiI CCGCTC 1 cut(s) 280
MboII GAAGA 1 cut(s) 376
MfeI CAATTG 1 cut(s) 350
MhlI GDGCHC 1 cut(s) 463
MluCI AATT 5 cut(s) 23, 98, 264, 350, 406
MnlI CCTC 2 cut(s) 137, 383
Mph1103I ATGCAT 1 cut(s) 160
MseI TTAA 4 cut(s) 6, 21, 42, 152
MslI CAYNNNNRTG 1 cut(s) 466
MunI CAATTG 1 cut(s) 350
Mva1269I GAATGC 1 cut(s) 422
NcoI CCATGG 1 cut(s) 273
NdeI CATATG 1 cut(s) 442
NlaIII CATG 3 cut(s) 55, 79, 277
NlaIV GGNNCC 1 cut(s) 220
NsiI ATGCAT 1 cut(s) 160
NspI RCATGY 2 cut(s) 55, 79
PciI ACATGT 1 cut(s) 51
PctI GAATGC 1 cut(s) 422
PfeI GAWTC 1 cut(s) 299
PkrI GCNGC 2 cut(s) 380, 383
Ppu21I YACGTR 1 cut(s) 455
PscI ACATGT 1 cut(s) 51
PspN4I GGNNCC 1 cut(s) 220
PstNI CAGNNNCTG 1 cut(s) 342
RsaI GTAC 3 cut(s) 94, 318, 453
RsaNI GTAC 3 cut(s) 93, 317, 452
RseI CAYNNNNRTG 1 cut(s) 466
SaqAI TTAA 4 cut(s) 6, 21, 42, 152
SatI GCNGC 2 cut(s) 379, 382
SduI GDGCHC 1 cut(s) 463
SetI ASST 4 cut(s) 315, 331, 457, 466
SfaNI GCATC 1 cut(s) 360
SfcI CTRYAG 1 cut(s) 294
SmiMI CAYNNNNRTG 1 cut(s) 466
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
Sse9I AATT 5 cut(s) 23, 98, 264, 350, 406
SsiI CCGC 2 cut(s) 280, 379
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 1 cut(s) 125
TaiI ACGT 1 cut(s) 457
TasI AATT 5 cut(s) 23, 98, 264, 350, 406
TatI WGTACW 2 cut(s) 92, 316
TauI GCSGC 1 cut(s) 381
TfiI GAWTC 1 cut(s) 299
Tru1I TTAA 4 cut(s) 6, 21, 42, 152
Tru9I TTAA 4 cut(s) 6, 21, 42, 152
TseI GCWGC 1 cut(s) 381
TspDTI ATGAA 1 cut(s) 212
TspGWI ACGGA 2 cut(s) 156, 179
VneI GTGCAC 1 cut(s) 459
XapI RAATTY 2 cut(s) 23, 98
XceI RCATGY 2 cut(s) 55, 79
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.