RchiOBHm_Chr5g0073051

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000183
Physical Location & Seq
Reverse (-)
79103450 .. 79111656
8207 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34796

Sequence Viewer

Length: 3084 bp
ATGGGTATGGGGGTGATGGGTTGCTGCACTTTCTCGTCGGACGACATTGTAATAAAGTCTCCTAATGATAAACGACTCTACAGAGTGATTAAGCTTGAGAATGGCCTTACTGCATTGCTCATTCACGATCCTCAGGTCTACCCACAAGGACCACCCCACCACTCCTCCGACCTTGAACAACAACAACAACAAGATAGTGAAGCTGTAGAAGAAGAAGAAGAAGAAGAGGAAGAAGAAGAGGAAGAGGAAGGTGAAGTTAAAAGGAAGGAAGAGGAGGGTGCTTCTGAGACTAAGAAGGCAGCAGCAGCAATGTGTGTCGGAATAGGCAGCTTCTCTGACCCTCTCGAGGCTCAGGGGCTTGCACATTTTCTAGAACACATGCTGTTCATGGGGAGTACAAAGTTCCCAGATGAAAATGAGTATGCTAGTTACTTGTCCAAGCATGGAGGGTCGTCAAATGCATATACAGAAGCAGAACATACTTGCTACTACTTTAATGTGAAACAAGAATTTCTTATGGGTGCCTTGAAAAGATTTTCTCAGTTCTTTGTTTCACCTCTAATGAAAAGTGAAGCCATGGAGAGAGAGGTTCAGGCTATAGATTCAGAGTTTAAAACGGTTCTGCAAAACGATTTCTCCCGCCTTCGACAACTTCAGGGCCATACATCCTCACCTGGTCACCCATTTAATAAATTCTCTTGGGGAAATAAGAAGAGCTTGGATGATGCAAAGGAAAAAGGGATAAACTTGCAGGAACGAATACTAAAATTGTACAGAGACTATTACCACGGTGGATTAATGAAGCTAGTTGTCATTGGTGGAGAATCTCTTGATGTACTTGAGCACTGGGTTTTGGAATTGTTTGGAGATGTCAAAAAAGGTCCCCAAGTAAATCTGGAGTTCAAGGCAGAAGGTCCTATTTGGAAAGCTGGAAAACTTTACAGGCTAGAGGCTGTTGACGATGTTCATATACTCCACCTAGCATGGACACTTCCATGTCTTCAAGAACACTATTTGAAGAAACCAGAAGATTACTTGAGTCATCTGCTTGGGCATGAGGGCAGGGGAAGTTTGCATTTCTACCTGAAAGCTAGAGGGTGGGTAACATCTTTAGATACTTGTTTGAGCGGGATGGACTGCTCTTCTGTGGCTTATATCTTTTGCATGGTCATATACCTCACTGACTCTGGATTGGAGAAGATTTTTGAAATAATTGGGTTCGTGTATCAATACATTGAGTTATTGCGTCAAATGTTGCCACAAGAATGGATATTTCGGGAACTTCAGGATATTGGGAACATGGACTTTAGATTTGCAGAGGAGCAGGATCAGGATGATTATGCTTCTCAACTTGCAGAAAATTTACTATATTATGCAACAGAGCATGTTATTTATGGGGACTATGTGAACGAGAGTTGGGACCAGGAATTGATAGAATATGTTCTTGGTTTCTTCAGACCAGAAAACATGAGGATTGATGTGATATCAAAGTCCTTATTTAAGTCAGAAGATTTCCAGTGTGAGCCTTGGTTTGGATCACATTATACTGAGGAAGATGTATCTCCATCTTTAATAAATTTATGGAAGAATCCTCAAGAAATTAGTGTTTCATTGCATCTGCCAGCAAAGAATGATTTCATTCCTCGTGATTTCTCCATCCGTTCTGATGTTTTGTGCATTGATACTGCAACGACATCTTGTCCGAGATGTATACTTGATGAACCATTGATGCAGTTTTGGTACAAGCTCGACAATACATTTAAACTTCCACATGTAAATACATATTTCCGCATCAATCTGAAGGGTGCATGTGATGATGTGAAGAGTTGTGTTTTGACTAGCTTATACCTTGACCTTCTCACAGATCAGCTGAATGAGATCCTCTATGAGGCCTGTGTTGCCAGCCTGGGAACTTCTTTTTCTCTGTCTCTGGACAAACTGCAGTTAGAGGTATACGGTTTCAATGATAAGCTTCCAGCTCTGTTGTCAAAAATTTTGGAAACAGTAAAAAGTTTCTTGCCAACTGATGATCGTTTTGAGGTTTTTAAAGAAGACATGGAGAGAGCATATACGAACGCCAATATGGATCCTTGGAGTTACTCAACATACTTGAGAGATCAAGTTCTGTTGAAGAAATTCTATACCATAGATGAGCAGTCGCATGTTTTGAAGGGATTGTCTGTTTCTGATCTGAAGTCTTTCATTCCTGAGATTTTTTCCCAGCTATACATTGAGGGCCTTTTGCATGGCAACTTGTCAGAAGAAGAAGCTATCAGTCTTGCAAAATTATTTCAAACAAATTTTACTGTACCACCACTTCCTAGCGAGTTGGTGTATAGAGACAATTGTATCTGTCTTCCTCCAAATGCTAACCTCATTAGAGATGCTACTGTGAAGAACAAGTCAGAAACAAACTCTGTGACTGAGCTGTATTTTCAAATTGAGCAAGCAGTGAAGATTGAGTCCATCAGACTAAAAGTATTGATTGATCTTTTTCATGAAATTGTACAGGAACCACTTTTTAATCAACTAAGGACAAAGGAGCAGCTTGGGTATGTTGTTCTCTGTGGCCGGAATCATACATGCAATGTTTTTGGCTTCTATTTCTGTGTTCAGTCGTCCGAGTACAACCCGATCCACCTTCAAGGCAGACTGGACAACTTTATCGATGGTCTGGAAGAGTTGTTGGAAGGACTGGAAGATGATTCCTTTGAGAATTATAAAGGTGGACTAATGGCAAAGATTCTGGAGAAAGATGCATCCCTCACATGTGAAACCAATCGATTGTGGACTCAGATTCTTGCTAAATGGTACAAGTTTGACTATTCGAAAAAGGCTGCAGAACAACTCAGAAGCGTTCAGAAGGAGGATGTTACCAAATTTTACAAGACCTATTTGCAACAATCATCTCCAAAGCGTCGAAGACTTGCAACTCGTGTTTGGGGTTGCAACACAGACTTGAAAGAAGCTGAAGAAGCGCGACCGGAGTCTGTGCAAGTCATTGAAGACCTTGCGGCCTTTAAGATGTCGTCCAAGTTTTATGATCGCAAAAGATGTCAAAAAGTGACTCCTCTAAATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

1027

Amino Acids

118.45

Weight (kDa)

4.94

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 96 - 218 1.8e-33 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 252 - 429 1.2e-17 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 436 - 719 2.5e-84 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 726 - 909 6.5e-17 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 831 - 930 1.2e-23 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2721
AccB1I GGYRCC 1 cut(s) 521
AccBSI CCGCTC 1 cut(s) 1128
AccI GTMKAC 3 cut(s) 138, 1711, 1953
AccII CGCG 1 cut(s) 2980
AciI CCGC 4 cut(s) 640, 1128, 1789, 3014
AclWI GGATC 7 cut(s) 122, 1335, 1543, 1873, 2081, 2094, 2629
AcoI YGGCCR 1 cut(s) 2569
AcsI RAATTY 8 cut(s) 509, 692, 1360, 1576, 1992, 2135, 2299, 2879
AcuI CTGAAG 6 cut(s) 638, 1268, 1438, 1820, 2213, 2991
AfaI GTAC 8 cut(s) 397, 773, 837, 1742, 2310, 2508, 2627, 2813
AfiI CCNNNNNNNGG 3 cut(s) 346, 1532, 1888
AflIII ACRYGT 2 cut(s) 1771, 2768
AhdI GACNNNNNGTC 1 cut(s) 1698
AjnI CCWGG 3 cut(s) 673, 1422, 1905
AjuI GAANNNNNNNTTGG 2 cut(s) 1428, 1460
Alw21I GWGCWC 1 cut(s) 846
Alw26I GTCTC 5 cut(s) 63, 281, 771, 1932, 2334
AlwI GGATC 7 cut(s) 122, 1335, 1543, 1873, 2081, 2094, 2629
Ama87I CYCGRG 1 cut(s) 344
AoxI GGCC 6 cut(s) 103, 658, 1890, 2236, 2569, 3015
ApeKI GCWGC 7 cut(s) 24, 299, 302, 305, 327, 2545, 2837
ApoI RAATTY 8 cut(s) 509, 692, 1360, 1576, 1992, 2135, 2299, 2879
ArsI GACNNNNNNTTYG 2 cut(s) 1295, 1327
AseI ATTAAT 1 cut(s) 797
Asp700I GAANNNNTTC 4 cut(s) 1440, 1634, 2135, 2198
AspLEI GCGC 1 cut(s) 2980
AspS9I GGNCC 6 cut(s) 149, 658, 881, 914, 1420, 2236
AsuHPI GGTGA 5 cut(s) 25, 263, 546, 663, 671
AsuII TTCGAA 1 cut(s) 2828
AvaI CYCGRG 1 cut(s) 344
AvaII GGWCC 4 cut(s) 149, 881, 914, 1420
AxyI CCTNAGG 1 cut(s) 132
BaeI ACNNNNGTAYC 2 cut(s) 2332, 2365
BamHI GGATCC 1 cut(s) 2086
BanI GGYRCC 1 cut(s) 521
BarI GAAGNNNNNNTAC 2 cut(s) 2301, 2333
BauI CACGAG 2 cut(s) 1644, 2934
BbsI GAAGAC 5 cut(s) 992, 2058, 2348, 2929, 3012
Bbv12I GWGCWC 1 cut(s) 846
BbvI GCAGC 7 cut(s) 11, 311, 314, 317, 339, 2557, 2824
BccI CCATC 6 cut(s) 10, 1126, 1573, 1664, 2474, 2663
BciT130I CCWGG 3 cut(s) 675, 1424, 1907
BcoDI GTCTC 5 cut(s) 63, 281, 771, 1932, 2334
BfaI CTAG 8 cut(s) 371, 426, 806, 947, 980, 1092, 1839, 2322
BfmI CTRYAG 5 cut(s) 79, 204, 597, 1940, 2838
BisI GCNGC 8 cut(s) 25, 300, 303, 306, 328, 2546, 2838, 3015
BlsI GCNGC 8 cut(s) 26, 301, 304, 307, 329, 2547, 2839, 3016
Bme1390I CCNGG 3 cut(s) 675, 1424, 1907
Bme18I GGWCC 4 cut(s) 149, 881, 914, 1420
BmeRI GACNNNNNGTC 1 cut(s) 1698
BmeT110I CYCGRG 1 cut(s) 344
BmgT120I GGNCC 6 cut(s) 149, 658, 881, 914, 1420, 2236
BmiI GGNNCC 5 cut(s) 523, 883, 1421, 2088, 2514
BmrFI CCNGG 3 cut(s) 675, 1424, 1907
BmrI ACTGGG 1 cut(s) 856
BmsI GCATC 7 cut(s) 715, 1624, 1719, 1800, 2374, 2746, 2768
BmuI ACTGGG 1 cut(s) 856
BoxI GACNNNNGTC 1 cut(s) 2986
BpiI GAAGAC 5 cut(s) 992, 2058, 2348, 2929, 3012
BpmI CTGGAG 2 cut(s) 917, 2768
Bpu10I CCTNAGC 1 cut(s) 351
Bpu14I TTCGAA 1 cut(s) 2828
BpuEI CTTGAG 5 cut(s) 116, 860, 1057, 1578, 2131
Bsa29I ATCGAT 2 cut(s) 2667, 2782
BsaJI CCNNGG 5 cut(s) 576, 787, 1526, 1906, 2090
BsaWI WCCGGW 1 cut(s) 2983
BsaXI ACNNNNNCTCC 4 cut(s) 149, 179, 620, 650
Bsc4I CCNNNNNNNGG 3 cut(s) 346, 1532, 1888
Bse1I ACTGG 4 cut(s) 851, 1516, 2658, 2700
Bse21I CCTNAGG 1 cut(s) 132
Bse3DI GCAATG 4 cut(s) 113, 315, 1610, 2593
BseBI CCWGG 3 cut(s) 675, 1424, 1907
BseCI ATCGAT 2 cut(s) 2667, 2782
BseDI CCNNGG 5 cut(s) 576, 787, 1526, 1906, 2090
BseGI GGATG 7 cut(s) 665, 727, 1137, 1339, 1656, 2759, 2875
BseLI CCNNNNNNNGG 3 cut(s) 346, 1532, 1888
BseMI GCAATG 4 cut(s) 113, 315, 1610, 2593
BseMII CTCAG 9 cut(s) 146, 276, 365, 554, 1539, 2199, 2415, 2807, 2863
BseNI ACTGG 4 cut(s) 851, 1516, 2658, 2700
BseRI GAGGAG 4 cut(s) 154, 287, 1334, 3060
BseXI GCAGC 7 cut(s) 11, 311, 314, 317, 339, 2557, 2824
BseYI CCCAGC 1 cut(s) 2220
BsgI GTGCAG 1 cut(s) 10
Bsh1236I CGCG 1 cut(s) 2980
Bsh1285I CGRYCG 1 cut(s) 2984
BshFI GGCC 6 cut(s) 105, 660, 1892, 2238, 2571, 3017
BshNI GGYRCC 1 cut(s) 521
BshVI ATCGAT 2 cut(s) 2667, 2782
BsiEI CGRYCG 1 cut(s) 2984
BsiHKAI GWGCWC 1 cut(s) 846
BsiHKCI CYCGRG 1 cut(s) 344
BsiSI CCGG 2 cut(s) 2572, 2984
BslFI GGGAC 3 cut(s) 867, 1412, 1433
BslI CCNNNNNNNGG 3 cut(s) 346, 1532, 1888
BsmAI GTCTC 5 cut(s) 63, 281, 771, 1932, 2334
BsmFI GGGAC 3 cut(s) 867, 1412, 1433
BsnI GGCC 6 cut(s) 105, 660, 1892, 2238, 2571, 3017
BsoBI CYCGRG 1 cut(s) 344
Bsp119I TTCGAA 1 cut(s) 2828
Bsp1286I GDGCHC 1 cut(s) 846
Bsp1407I TGTACA 2 cut(s) 771, 2506
Bsp19I CCATGG 1 cut(s) 576
BspACI CCGC 4 cut(s) 640, 1128, 1789, 3014
BspANI GGCC 6 cut(s) 105, 660, 1892, 2238, 2571, 3017
BspCNI CTCAG 9 cut(s) 145, 277, 364, 553, 1540, 2200, 2416, 2806, 2862
BspDI ATCGAT 2 cut(s) 2667, 2782
BspFNI CGCG 1 cut(s) 2980
BspHI TCATGA 1 cut(s) 2497
BspLI GGNNCC 5 cut(s) 523, 883, 1421, 2088, 2514
BspMAI CTGCAG 2 cut(s) 1944, 2842
BspPI GGATC 7 cut(s) 122, 1335, 1543, 1873, 2081, 2094, 2629
BspQI GCTCTTC 2 cut(s) 707, 1147
BspT104I TTCGAA 1 cut(s) 2828
BspT107I GGYRCC 1 cut(s) 521
BsrBI CCGCTC 1 cut(s) 1128
BsrDI GCAATG 4 cut(s) 113, 315, 1610, 2593
BsrGI TGTACA 2 cut(s) 771, 2506
BsrI ACTGG 4 cut(s) 851, 1516, 2658, 2700
BssECI CCNNGG 5 cut(s) 576, 787, 1526, 1906, 2090
BssNAI GTATAC 2 cut(s) 1712, 1954
BssSI CACGAG 2 cut(s) 1644, 2934
BssT1I CCWWGG 3 cut(s) 576, 1526, 2090
Bst1107I GTATAC 2 cut(s) 1712, 1954
Bst2BI CACGAG 2 cut(s) 1644, 2934
Bst2UI CCWGG 3 cut(s) 675, 1424, 1907
Bst4CI ACNGT 6 cut(s) 619, 791, 1958, 2005, 2308, 2392
Bst6I CTCTTC 8 cut(s) 219, 231, 237, 264, 707, 1147, 1817, 2673
BstAUI TGTACA 2 cut(s) 771, 2506
BstBI TTCGAA 1 cut(s) 2828
BstC8I GCNNGC 4 cut(s) 360, 1623, 1903, 2448
BstDSI CCRYGG 2 cut(s) 576, 787
BstEII GGTNACC 1 cut(s) 677
BstENI CCTNNNNNAGG 1 cut(s) 1886
BstF5I GGATG 7 cut(s) 665, 727, 1137, 1339, 1656, 2759, 2875
BstFNI CGCG 1 cut(s) 2980
BstHHI GCGC 1 cut(s) 2980
BstMAI GTCTC 5 cut(s) 63, 281, 771, 1932, 2334
BstMCI CGRYCG 1 cut(s) 2984
BstMWI GCNNNNNNNGC 3 cut(s) 305, 1898, 2975
BstNI CCWGG 3 cut(s) 675, 1424, 1907
BstNSI RCATGY 7 cut(s) 382, 1388, 1775, 1812, 2165, 2586, 2772
BstPAI GACNNNNGTC 1 cut(s) 2986
BstPI GGTNACC 1 cut(s) 677
BstSCI CCNGG 3 cut(s) 673, 1422, 1905
BstSFI CTRYAG 5 cut(s) 79, 204, 597, 1940, 2838
BstUI CGCG 1 cut(s) 2980
BstV1I GCAGC 7 cut(s) 11, 311, 314, 317, 339, 2557, 2824
BstV2I GAAGAC 5 cut(s) 992, 2058, 2348, 2929, 3012
BstX2I RGATCY 2 cut(s) 1878, 2086
BstXI CCANNNNNNTGG 1 cut(s) 1266
BstYI RGATCY 2 cut(s) 1878, 2086
BstZ17I GTATAC 2 cut(s) 1712, 1954
Bsu15I ATCGAT 2 cut(s) 2667, 2782
Bsu36I CCTNAGG 1 cut(s) 132
BsuRI GGCC 6 cut(s) 105, 660, 1892, 2238, 2571, 3017
BsuTUI ATCGAT 2 cut(s) 2667, 2782
BtgI CCRYGG 2 cut(s) 576, 787
BtsCI GGATG 7 cut(s) 665, 727, 1137, 1339, 1656, 2759, 2875
BtsI GCAGTG 1 cut(s) 2457
BtsIMutI CAGTG 4 cut(s) 844, 1179, 1523, 2457
Cac8I GCNNGC 4 cut(s) 360, 1623, 1903, 2448
CciI TCATGA 1 cut(s) 2497
CfoI GCGC 1 cut(s) 2980
Cfr13I GGNCC 6 cut(s) 149, 658, 881, 914, 1420, 2236
ClaI ATCGAT 2 cut(s) 2667, 2782
CseI GACGC 2 cut(s) 1235, 2906
CsiI ACCWGGT 1 cut(s) 673
Csp6I GTAC 8 cut(s) 396, 772, 836, 1741, 2309, 2507, 2626, 2812
CviQI GTAC 8 cut(s) 396, 772, 836, 1741, 2309, 2507, 2626, 2812
DraI TTTAAA 3 cut(s) 613, 1762, 2047
DriI GACNNNNNGTC 1 cut(s) 1698
EaeI YGGCCR 1 cut(s) 2569
Eam1104I CTCTTC 8 cut(s) 219, 231, 237, 264, 707, 1147, 1817, 2673
Eam1105I GACNNNNNGTC 1 cut(s) 1698
EarI CTCTTC 8 cut(s) 219, 231, 237, 264, 707, 1147, 1817, 2673
Eco130I CCWWGG 3 cut(s) 576, 1526, 2090
Eco147I AGGCCT 1 cut(s) 1892
Eco32I GATATC 1 cut(s) 1485
Eco47I GGWCC 4 cut(s) 149, 881, 914, 1420
Eco57I CTGAAG 6 cut(s) 638, 1268, 1438, 1820, 2213, 2991
Eco81I CCTNAGG 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 344
Eco91I GGTNACC 1 cut(s) 677
EcoNI CCTNNNNNAGG 1 cut(s) 1886
EcoO109I RGGNCCY 3 cut(s) 881, 914, 2236
EcoO65I GGTNACC 1 cut(s) 677
EcoRII CCWGG 3 cut(s) 673, 1422, 1905
EcoRV GATATC 1 cut(s) 1485
EcoT14I CCWWGG 3 cut(s) 576, 1526, 2090
EcoT22I ATGCAT 2 cut(s) 463, 2761
ErhI CCWWGG 3 cut(s) 576, 1526, 2090
FalI AAGNNNNNCTT 4 cut(s) 498, 530, 701, 733
FaqI GGGAC 3 cut(s) 867, 1412, 1433
FauI CCCGC 2 cut(s) 647, 1121
FblI GTMKAC 3 cut(s) 138, 1711, 1953
Fnu4HI GCNGC 8 cut(s) 25, 300, 303, 306, 328, 2546, 2838, 3015
FokI GGATG 7 cut(s) 652, 734, 1144, 1346, 1643, 2746, 2882
Fsp4HI GCNGC 8 cut(s) 25, 300, 303, 306, 328, 2546, 2838, 3015
FspBI CTAG 8 cut(s) 371, 426, 806, 947, 980, 1092, 1839, 2322
GlaI GCGC 1 cut(s) 2979
GluI GCNGC 8 cut(s) 25, 300, 303, 306, 328, 2546, 2838, 3015
GsaI CCCAGC 1 cut(s) 2224
GsuI CTGGAG 2 cut(s) 917, 2768
HaeIII GGCC 6 cut(s) 105, 660, 1892, 2238, 2571, 3017
HapII CCGG 2 cut(s) 2572, 2984
HgaI GACGC 2 cut(s) 1235, 2906
HhaI GCGC 1 cut(s) 2980
Hin6I GCGC 1 cut(s) 2978
HinP1I GCGC 1 cut(s) 2978
HincII GTYRAC 1 cut(s) 958
HindII GTYRAC 1 cut(s) 958
HindIII AAGCTT 2 cut(s) 92, 1970
HpaII CCGG 2 cut(s) 2572, 2984
HphI GGTGA 5 cut(s) 25, 263, 546, 663, 671
Hpy166II GTNNAC 7 cut(s) 139, 958, 1408, 1712, 1954, 2729, 2790
Hpy8I GTNNAC 7 cut(s) 139, 958, 1408, 1712, 1954, 2729, 2790
Hpy99I CGWCG 2 cut(s) 40, 2922
HpyCH4III ACNGT 6 cut(s) 619, 791, 1958, 2005, 2308, 2392
HpyF10VI GCNNNNNNNGC 3 cut(s) 305, 1898, 2975
HspAI GCGC 1 cut(s) 2978
LguI GCTCTTC 2 cut(s) 707, 1147
LmnI GCTCC 2 cut(s) 1321, 2542
Lsp1109I GCAGC 7 cut(s) 11, 311, 314, 317, 339, 2557, 2824
LweI GCATC 7 cut(s) 715, 1624, 1719, 1800, 2374, 2746, 2768
MabI ACCWGGT 1 cut(s) 673
MaeI CTAG 8 cut(s) 371, 426, 806, 947, 980, 1092, 1839, 2322
MaeIII GTNAC 7 cut(s) 428, 677, 1102, 2096, 2419, 2872, 3064
MbiI CCGCTC 1 cut(s) 1128
MfeI CAATTG 1 cut(s) 2344
MflI RGATCY 2 cut(s) 1878, 2086
MhlI GDGCHC 1 cut(s) 846
MlyI GAGTC 7 cut(s) 69, 1048, 1178, 2471, 2785, 2996, 3061
MmeI TCCRAC 4 cut(s) 18, 192, 298, 2667
Mph1103I ATGCAT 2 cut(s) 463, 2761
MroXI GAANNNNTTC 4 cut(s) 1440, 1634, 2135, 2198
MslI CAYNNNNRTG 2 cut(s) 994, 1264
MspA1I CMGCKG 1 cut(s) 1870
MspI CCGG 2 cut(s) 2572, 2984
MspR9I CCNGG 3 cut(s) 675, 1424, 1907
MunI CAATTG 1 cut(s) 2344
MvaI CCWGG 3 cut(s) 675, 1424, 1907
MvnI CGCG 1 cut(s) 2980
MwoI GCNNNNNNNGC 3 cut(s) 305, 1898, 2975
NcoI CCATGG 1 cut(s) 576
NlaIV GGNNCC 5 cut(s) 523, 883, 1421, 2088, 2514
NmuCI GTSAC 3 cut(s) 677, 2419, 3064
NsiI ATGCAT 2 cut(s) 463, 2761
NspI RCATGY 7 cut(s) 382, 1388, 1775, 1812, 2165, 2586, 2772
NspV TTCGAA 1 cut(s) 2828
PaeR7I CTCGAG 1 cut(s) 344
PagI TCATGA 1 cut(s) 2497
PceI AGGCCT 1 cut(s) 1892
PciI ACATGT 2 cut(s) 1771, 2768
PciSI GCTCTTC 2 cut(s) 707, 1147
PdmI GAANNNNTTC 4 cut(s) 1440, 1634, 2135, 2198
PfeI GAWTC 7 cut(s) 602, 824, 1588, 2575, 2705, 2743, 2797
PkrI GCNGC 8 cut(s) 26, 301, 304, 307, 329, 2547, 2839, 3016
PleI GAGTC 7 cut(s) 69, 1047, 1178, 2470, 2785, 2995, 3061
PpsI GAGTC 7 cut(s) 69, 1047, 1178, 2470, 2785, 2995, 3061
PpuMI RGGWCCY 2 cut(s) 881, 914
PscI ACATGT 2 cut(s) 1771, 2768
PshAI GACNNNNGTC 1 cut(s) 2986
PshBI ATTAAT 1 cut(s) 797
PsiI TTATAA 1 cut(s) 2721
Psp5II RGGWCCY 2 cut(s) 881, 914
Psp6I CCWGG 3 cut(s) 673, 1422, 1905
PspEI GGTNACC 1 cut(s) 677
PspFI CCCAGC 1 cut(s) 2220
PspGI CCWGG 3 cut(s) 673, 1422, 1905
PspN4I GGNNCC 5 cut(s) 523, 883, 1421, 2088, 2514
PspPI GGNCC 6 cut(s) 149, 658, 881, 914, 1420, 2236
PspPPI RGGWCCY 2 cut(s) 881, 914
PstI CTGCAG 2 cut(s) 1944, 2842
PsuI RGATCY 2 cut(s) 1878, 2086
PvuII CAGCTG 1 cut(s) 1870
RsaI GTAC 8 cut(s) 397, 773, 837, 1742, 2310, 2508, 2627, 2813
RsaNI GTAC 8 cut(s) 396, 772, 836, 1741, 2309, 2507, 2626, 2812
RseI CAYNNNNRTG 2 cut(s) 994, 1264
SapI GCTCTTC 2 cut(s) 707, 1147
SatI GCNGC 8 cut(s) 25, 300, 303, 306, 328, 2546, 2838, 3015
Sau96I GGNCC 6 cut(s) 149, 658, 881, 914, 1420, 2236
SchI GAGTC 7 cut(s) 69, 1048, 1178, 2471, 2785, 2996, 3061
ScrFI CCNGG 3 cut(s) 675, 1424, 1907
SduI GDGCHC 1 cut(s) 846
SexAI ACCWGGT 1 cut(s) 673
SfaNI GCATC 7 cut(s) 715, 1624, 1719, 1800, 2374, 2746, 2768
SfcI CTRYAG 5 cut(s) 79, 204, 597, 1940, 2838
Sfr274I CTCGAG 1 cut(s) 344
SfuI TTCGAA 1 cut(s) 2828
SinI GGWCC 4 cut(s) 149, 881, 914, 1420
SlaI CTCGAG 1 cut(s) 344
SmiMI CAYNNNNRTG 2 cut(s) 994, 1264
SmlI CTYRAG 6 cut(s) 95, 344, 839, 1036, 1593, 2110
SmoI CTYRAG 6 cut(s) 95, 344, 839, 1036, 1593, 2110
SseBI AGGCCT 1 cut(s) 1892
SsiI CCGC 4 cut(s) 640, 1128, 1789, 3014
SspMI CTAG 8 cut(s) 371, 426, 806, 947, 980, 1092, 1839, 2322
StuI AGGCCT 1 cut(s) 1892
StyD4I CCNGG 3 cut(s) 673, 1422, 1905
StyI CCWWGG 3 cut(s) 576, 1526, 2090
TaaI ACNGT 6 cut(s) 619, 791, 1958, 2005, 2308, 2392
TaqI TCGA 7 cut(s) 345, 646, 1749, 2667, 2782, 2828, 2920
TatI WGTACW 5 cut(s) 395, 771, 835, 2506, 2625
TauI GCSGC 1 cut(s) 3017
TfiI GAWTC 7 cut(s) 602, 824, 1588, 2575, 2705, 2743, 2797
TscAI CASTG 4 cut(s) 851, 1186, 1523, 2457
TseFI GTSAC 3 cut(s) 677, 2419, 3064
TseI GCWGC 7 cut(s) 24, 299, 302, 305, 327, 2545, 2837
Tsp45I GTSAC 3 cut(s) 677, 2419, 3064
TspGWI ACGGA 1 cut(s) 1649
TspRI CASTG 4 cut(s) 851, 1186, 1523, 2457
VpaK11BI GGWCC 4 cut(s) 149, 881, 914, 1420
VspI ATTAAT 1 cut(s) 797
XagI CCTNNNNNAGG 1 cut(s) 1886
XapI RAATTY 8 cut(s) 509, 692, 1360, 1576, 1992, 2135, 2299, 2879
XbaI TCTAGA 1 cut(s) 370
XceI RCATGY 7 cut(s) 382, 1388, 1775, 1812, 2165, 2586, 2772
XhoI CTCGAG 1 cut(s) 344
XmiI GTMKAC 3 cut(s) 138, 1711, 1953
XmnI GAANNNNTTC 4 cut(s) 1440, 1634, 2135, 2198
XspI CTAG 8 cut(s) 371, 426, 806, 947, 980, 1092, 1839, 2322
Zsp2I ATGCAT 2 cut(s) 463, 2761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.