Rh5DG501800

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000183
Physical Location & Seq
Reverse (-)
78611881 .. 78630032
18152 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG501800.1

Sequence Viewer

Length: 888 bp
ATGGAAGGGACAAGTACATCCCCAAAACAGGGTGTTCTGGAGTGTACTCTATTGCCTCCCAATGTACATTCGAAGGGAATAAAACCGAAACAAGAACTCTATAGACTGACATCTTCCCTTCCGATGCTGGAGATGCAGATGGCTATGATTTTCATCACAACCCAAGTCCTCCATTTACTTTTGAAGAGGTTTCATATCTCCAAGTTCAGCAGCCATCTTTTTACAGGCATACTCTTCGGCGTGCTTTACCACTATTGGGGATTTGGTGAAAAAGCATTTAATGTGAAAAGTCAAGGAGTTATTAGTCTTTTGGGTGAGCTTGGGTACGCCCTCTTCCTGTTTGTACTCGGAACGACAATGGATTTGGGAATGATAAGAAGATCAGGATCTAAAACCATGCTCACCGGGGTTTTATGCATGTTGATCCCTTTGGCAAGTGGTGCGGTTGTCCAAGTGAAATATGTCTATAACCTTAACAAGAGAGACAACAATCAGCTTGAGCTTGTGACCGCTACGCATAGCATAACTGCGTTTCCGGTTGTTGTTCTCCTTCTCGAGGACCTGAAGCTCCTCAATTCTGAAATTGGCCGGTTAAGCCAGTCTGCAACATTAGTCTGTGACGTCTTTACTGATGCATTTGTTCTTGCTCTCATTTTAAGCTGCAAAGGAATTGTTCAGCTTTCAGCCTATTCTAGGTTCAGAGACTTCCGGATAGTAAATGATTCGGTGTATGCTGTCATGTTTTCTAGCGTTGTAGTGACTTCAACTCGTGCCTATTATAGTGAAGCACCTCTACGATCCTTCAAGAAAATACGCAGGCTACGAGAGAAGGGACATTATGCATTTGAAACCCAATGCAGAGCTCAAAATTCTTACCTGCATCCATAG

Protein Analysis

295

Amino Acids

33.11

Weight (kDa)

9.53

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 60 - 210 5.3e-12 Sodium/hydrogen exchanger, transmembrane
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 624
AccIII TCCGGA 1 cut(s) 708
AciI CCGC 2 cut(s) 443, 510
AclWI GGATC 3 cut(s) 394, 418, 792
AcoI YGGCCR 1 cut(s) 586
AcsI RAATTY 1 cut(s) 868
AcuI CTGAAG 1 cut(s) 584
AcyI GRCGYC 1 cut(s) 621
AfaI GTAC 5 cut(s) 16, 46, 66, 326, 345
AfiI CCNNNNNNNGG 5 cut(s) 28, 29, 256, 556, 693
AgsI TTSAA 4 cut(s) 184, 765, 805, 848
AluBI AGCT 7 cut(s) 319, 496, 502, 568, 660, 679, 863
AluI AGCT 7 cut(s) 319, 496, 502, 568, 660, 679, 863
Alw21I GWGCWC 1 cut(s) 865
Alw26I GTCTC 2 cut(s) 477, 696
AlwI GGATC 3 cut(s) 394, 418, 792
Ama87I CYCGRG 1 cut(s) 554
Aor13HI TCCGGA 1 cut(s) 708
AoxI GGCC 1 cut(s) 586
ApeKI GCWGC 2 cut(s) 210, 660
ApoI RAATTY 1 cut(s) 868
ArsI GACNNNNNNTTYG 2 cut(s) 346, 378
AspS9I GGNCC 1 cut(s) 559
AsuC2I CCSGG 1 cut(s) 406
AsuHPI GGTGA 3 cut(s) 278, 326, 394
AsuII TTCGAA 1 cut(s) 71
AvaI CYCGRG 1 cut(s) 554
AvaII GGWCC 1 cut(s) 559
BanII GRGCYC 1 cut(s) 865
BarI GAAGNNNNNNTAC 4 cut(s) 317, 349, 777, 809
BauI CACGAG 1 cut(s) 768
Bbv12I GWGCWC 1 cut(s) 865
BbvI GCAGC 2 cut(s) 222, 647
BccI CCATC 2 cut(s) 133, 222
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BcnI CCSGG 1 cut(s) 406
BcoDI GTCTC 2 cut(s) 477, 696
BfaI CTAG 2 cut(s) 693, 747
BfmI CTRYAG 1 cut(s) 100
BisI GCNGC 2 cut(s) 211, 661
BlsI GCNGC 2 cut(s) 212, 662
Bme1390I CCNGG 1 cut(s) 406
Bme18I GGWCC 1 cut(s) 559
BmeT110I CYCGRG 1 cut(s) 554
BmgT120I GGNCC 1 cut(s) 559
BmrFI CCNGG 1 cut(s) 406
BmsI GCATC 3 cut(s) 114, 123, 622
BpmI CTGGAG 2 cut(s) 59, 149
Bpu14I TTCGAA 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 518
BpuMI CCSGG 1 cut(s) 406
BsaBI GATNNNNATC 2 cut(s) 152, 385
BsaHI GRCGYC 1 cut(s) 621
BsaJI CCNNGG 1 cut(s) 405
BsaWI WCCGGW 2 cut(s) 535, 708
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 29, 256, 556, 693
Bse118I RCCGGY 1 cut(s) 588
Bse1I ACTGG 1 cut(s) 598
Bse8I GATNNNNATC 2 cut(s) 152, 385
BseAI TCCGGA 1 cut(s) 708
BseDI CCNNGG 1 cut(s) 405
BseGI GGATG 2 cut(s) 17, 880
BseJI GATNNNNATC 2 cut(s) 152, 385
BseLI CCNNNNNNNGG 5 cut(s) 28, 29, 256, 556, 693
BseNI ACTGG 1 cut(s) 598
BseRI GAGGAG 1 cut(s) 560
BseXI GCAGC 2 cut(s) 222, 647
BshFI GGCC 1 cut(s) 588
BsiHKAI GWGCWC 1 cut(s) 865
BsiHKCI CYCGRG 1 cut(s) 554
BsiSI CCGG 4 cut(s) 405, 536, 589, 709
BslFI GGGAC 2 cut(s) 22, 846
BslI CCNNNNNNNGG 5 cut(s) 28, 29, 256, 556, 693
BsmAI GTCTC 2 cut(s) 477, 696
BsmFI GGGAC 2 cut(s) 22, 846
BsnI GGCC 1 cut(s) 588
BsoBI CYCGRG 1 cut(s) 554
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 865
Bsp13I TCCGGA 1 cut(s) 708
Bsp1407I TGTACA 1 cut(s) 64
Bsp143I GATC 4 cut(s) 380, 386, 423, 797
BspACI CCGC 2 cut(s) 443, 510
BspANI GGCC 1 cut(s) 588
BspEI TCCGGA 1 cut(s) 708
BspPI GGATC 3 cut(s) 394, 418, 792
BspT104I TTCGAA 1 cut(s) 71
BsrFI RCCGGY 1 cut(s) 588
BsrGI TGTACA 1 cut(s) 64
BsrI ACTGG 1 cut(s) 598
BssAI RCCGGY 1 cut(s) 588
BssECI CCNNGG 1 cut(s) 405
BssMI GATC 4 cut(s) 380, 386, 423, 797
BssNI GRCGYC 1 cut(s) 621
BssSI CACGAG 1 cut(s) 768
Bst2BI CACGAG 1 cut(s) 768
Bst6I CTCTTC 3 cut(s) 179, 239, 338
BstACI GRCGYC 1 cut(s) 621
BstAPI GCANNNNNTGC 1 cut(s) 440
BstAUI TGTACA 1 cut(s) 64
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 2 cut(s) 242, 818
BstENI CCTNNNNNAGG 2 cut(s) 554, 691
BstF5I GGATG 2 cut(s) 17, 880
BstKTI GATC 4 cut(s) 383, 389, 426, 800
BstMAI GTCTC 2 cut(s) 477, 696
BstMBI GATC 4 cut(s) 380, 386, 423, 797
BstMWI GCNNNNNNNGC 3 cut(s) 133, 440, 594
BstNSI RCATGY 1 cut(s) 421
BstSCI CCNGG 1 cut(s) 404
BstSFI CTRYAG 1 cut(s) 100
BstV1I GCAGC 2 cut(s) 222, 647
BstX2I RGATCY 1 cut(s) 386
BstYI RGATCY 1 cut(s) 386
BsuRI GGCC 1 cut(s) 588
BtsCI GGATG 2 cut(s) 17, 880
Cac8I GCNNGC 2 cut(s) 242, 818
Cfr10I RCCGGY 1 cut(s) 588
Cfr13I GGNCC 1 cut(s) 559
Csp6I GTAC 5 cut(s) 15, 45, 65, 325, 344
CviAII CATG 3 cut(s) 397, 418, 739
CviQI GTAC 5 cut(s) 15, 45, 65, 325, 344
DpnI GATC 4 cut(s) 382, 388, 425, 799
DpnII GATC 4 cut(s) 380, 386, 423, 797
EaeI YGGCCR 1 cut(s) 586
Eam1104I CTCTTC 3 cut(s) 179, 239, 338
EarI CTCTTC 3 cut(s) 179, 239, 338
Ecl136II GAGCTC 1 cut(s) 863
Eco24I GRGCYC 1 cut(s) 865
Eco47I GGWCC 1 cut(s) 559
Eco53kI GAGCTC 1 cut(s) 863
Eco57I CTGAAG 1 cut(s) 584
Eco88I CYCGRG 1 cut(s) 554
EcoICRI GAGCTC 1 cut(s) 863
EcoNI CCTNNNNNAGG 2 cut(s) 554, 691
EcoO109I RGGNCCY 1 cut(s) 559
EcoT22I ATGCAT 3 cut(s) 419, 637, 844
EcoT38I GRGCYC 1 cut(s) 865
FaeI CATG 3 cut(s) 400, 421, 742
FaqI GGGAC 2 cut(s) 22, 846
FatI CATG 3 cut(s) 396, 417, 738
Fnu4HI GCNGC 2 cut(s) 211, 661
FokI GGATG 2 cut(s) 4, 867
FriOI GRGCYC 1 cut(s) 865
Fsp4HI GCNGC 2 cut(s) 211, 661
FspBI CTAG 2 cut(s) 693, 747
GluI GCNGC 2 cut(s) 211, 661
GsuI CTGGAG 2 cut(s) 59, 149
HaeIII GGCC 1 cut(s) 588
HapII CCGG 4 cut(s) 405, 536, 589, 709
Hin1I GRCGYC 1 cut(s) 621
Hin1II CATG 3 cut(s) 400, 421, 742
HinfI GANTC 1 cut(s) 722
HpaII CCGG 4 cut(s) 405, 536, 589, 709
HphI GGTGA 3 cut(s) 278, 326, 394
Hpy166II GTNNAC 1 cut(s) 45
Hpy188I TCNGA 4 cut(s) 123, 350, 580, 701
Hpy188III TCNNGA 5 cut(s) 38, 384, 554, 709, 805
Hpy8I GTNNAC 1 cut(s) 45
HpyAV CCTTC 5 cut(s) 67, 128, 560, 811, 823
HpyCH4IV ACGT 1 cut(s) 621
HpyCH4V TGCA 8 cut(s) 136, 417, 605, 635, 663, 842, 858, 880
HpyF10VI GCNNNNNNNGC 3 cut(s) 133, 440, 594
HpySE526I ACGT 1 cut(s) 621
Hsp92I GRCGYC 1 cut(s) 621
Hsp92II CATG 3 cut(s) 400, 421, 742
Kpn2I TCCGGA 1 cut(s) 708
Kzo9I GATC 4 cut(s) 380, 386, 423, 797
LmnI GCTCC 1 cut(s) 573
Lsp1109I GCAGC 2 cut(s) 222, 647
LweI GCATC 3 cut(s) 114, 123, 622
MaeI CTAG 2 cut(s) 693, 747
MaeII ACGT 1 cut(s) 621
MaeIII GTNAC 3 cut(s) 505, 617, 757
MalI GATC 4 cut(s) 382, 388, 425, 799
MboI GATC 4 cut(s) 380, 386, 423, 797
MboII GAAGA 5 cut(s) 105, 196, 226, 325, 390
MflI RGATCY 1 cut(s) 386
MhlI GDGCHC 1 cut(s) 865
MluCI AATT 4 cut(s) 574, 582, 669, 868
MnlI CCTC 7 cut(s) 66, 179, 180, 341, 550, 581, 801
Mph1103I ATGCAT 3 cut(s) 419, 637, 844
MroI TCCGGA 1 cut(s) 708
MseI TTAA 4 cut(s) 279, 474, 593, 656
MspI CCGG 4 cut(s) 405, 536, 589, 709
MspR9I CCNGG 1 cut(s) 406
MwoI GCNNNNNNNGC 3 cut(s) 133, 440, 594
NciI CCSGG 1 cut(s) 406
NdeII GATC 4 cut(s) 380, 386, 423, 797
NlaIII CATG 3 cut(s) 400, 421, 742
NmuCI GTSAC 3 cut(s) 505, 617, 757
NsiI ATGCAT 3 cut(s) 419, 637, 844
NspI RCATGY 1 cut(s) 421
NspV TTCGAA 1 cut(s) 71
PaeR7I CTCGAG 1 cut(s) 554
PcsI WCGNNNNNNNCGW 1 cut(s) 820
PfeI GAWTC 1 cut(s) 722
PkrI GCNGC 2 cut(s) 212, 662
PpuMI RGGWCCY 1 cut(s) 559
Psp124BI GAGCTC 1 cut(s) 865
Psp5II RGGWCCY 1 cut(s) 559
PspPI GGNCC 1 cut(s) 559
PspPPI RGGWCCY 1 cut(s) 559
PsuI RGATCY 1 cut(s) 386
RsaI GTAC 5 cut(s) 16, 46, 66, 326, 345
RsaNI GTAC 5 cut(s) 15, 45, 65, 325, 344
SacI GAGCTC 1 cut(s) 865
SaqAI TTAA 4 cut(s) 279, 474, 593, 656
SatI GCNGC 2 cut(s) 211, 661
Sau3AI GATC 4 cut(s) 380, 386, 423, 797
Sau96I GGNCC 1 cut(s) 559
ScrFI CCNGG 1 cut(s) 406
SduI GDGCHC 1 cut(s) 865
SfaNI GCATC 3 cut(s) 114, 123, 622
SfcI CTRYAG 1 cut(s) 100
Sfr274I CTCGAG 1 cut(s) 554
SfuI TTCGAA 1 cut(s) 71
SinI GGWCC 1 cut(s) 559
SlaI CTCGAG 1 cut(s) 554
SmlI CTYRAG 2 cut(s) 497, 554
SmoI CTYRAG 2 cut(s) 497, 554
Sse9I AATT 4 cut(s) 574, 582, 669, 868
SsiI CCGC 2 cut(s) 443, 510
SspMI CTAG 2 cut(s) 693, 747
SstI GAGCTC 1 cut(s) 865
StyD4I CCNGG 1 cut(s) 404
TaiI ACGT 1 cut(s) 624
TaqI TCGA 2 cut(s) 71, 555
TasI AATT 4 cut(s) 574, 582, 669, 868
TatI WGTACW 4 cut(s) 14, 44, 64, 343
TfiI GAWTC 1 cut(s) 722
Tru1I TTAA 4 cut(s) 279, 474, 593, 656
Tru9I TTAA 4 cut(s) 279, 474, 593, 656
TseFI GTSAC 3 cut(s) 505, 617, 757
TseI GCWGC 2 cut(s) 210, 660
Tsp45I GTSAC 3 cut(s) 505, 617, 757
TspDTI ATGAA 2 cut(s) 142, 182
VpaK11BI GGWCC 1 cut(s) 559
XagI CCTNNNNNAGG 2 cut(s) 554, 691
XapI RAATTY 1 cut(s) 868
XceI RCATGY 1 cut(s) 421
XhoI CTCGAG 1 cut(s) 554
XspI CTAG 2 cut(s) 693, 747
ZraI GACGTC 1 cut(s) 622
Zsp2I ATGCAT 3 cut(s) 419, 637, 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.