Rh5AG477200

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000183
Physical Location & Seq
Reverse (-)
81993725 .. 82000693
6969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG477200.1

Sequence Viewer

Length: 1854 bp
ATGGGTCGCTGCACTTTCTCCTCGGACGATATTGTAATAAAGTCTCCGAATGATAAGAAATTGTACAGACTAATTGAGCTTGAGAATGGCCTCACTGCATTGCTCATTCATGATCCTGAGATTTACCCACAAGGGCCCAAAAGCCTTGAACCCAGTGAAGAAGATGAAGCAGCCAAAGGTGGAGATGGTGCCTCTGAGACTAAGACGGCAGCAGCAGCAATGTGCGTTGGAATAGGCAGCATGTCTGACCCTGTTGAGGCACAAGGGCTTGCACACTTCCTAGAACACATGCTCTTTATGGGGAGTACAAAGTTTCCTATTGAAAATGAGTTCGGTAGTTACTTGTCCAAGCATGGAGGATGGTCAAATGCATACACAGAAGCAGAGCAGACTTGCTACTATTTTGTTGTAAAACGAGAGTTTCTAAAGGGTGCCTTATCAAGATTTTCTCAGTTCTTTGTTTCACCTCTAATGAAAAGTGAAGCCATGGAGCGAGAGTTACAGGCTATAGATTCAGGAAATTTACTACATTATCCCGCAGCGCATGCTATTTATGGGGACTATTTGCTAGAGAGTTGGGATGAGAAATTGATAGAATATACTCTAGGTTTCTTGAGACCAGAAAACATGAGGATTGATGTGATATCTAAGTCCTCCACGTTGTCAGAAGATTTCCAGTGCGAGCCTTGGTTTGGGTCACATTTTACCGAGGAAGATATATCTCCGTCTTTGATAGATTTATGGAAGGATCCTCAAGAAATTGATAGTTCATTGCATCTCCCAGAAAATAATGAATTCATTCCCTCTGATTTTTCCATCCGTTCTGATGTTCTTGATACCGCAAATACATCTTGTCCGAGATGTATACTTGATGGGCCATTGGTGAAGTTATGGTACAAGCTTGATAGTACTTACAAACTTCCGCAGGCAAACATATACTTTCGCGTCAGTCTAAAGGAAGCATCTGGTAGTGTGAAGAATTCTGTCTTGACTCAACTATATGGCGACCTTCTTTGGGATGAGCTGAATGAGGTTGTATATCAGGCTAGTGTCGCCCAGCTGGGAACTTCTGTATCTGTGTACACTAACTATCTGGAGCTGAAGGTCAGCGGTTTCAATGATAAGATTCCAACTCTCTTGTCAAAAATTATGACAACAGCCAAAAATTTCTCGCCAACTTACGAATGTTTCAAGGTTATTAAAGAGGACATGGAGCGAGCATATACGAACACCAATATGGATCCTTGGAGCCACTCGACATACTTGAGAGATGAAGTTTTGTACCAAAATTTCAGTGATGTAGATGAGACGTTGCATGTTTTAAATGGATTGACTGTTTCTGATGTGAAGTCATTCATTCCCGAGCTTTGGTCCCCGTCGTCTGATTACAATCCAATCTACTTACAAATAAGAGTTGACAACTTTATCAATGGTATGGAAGAGCTTTTGCTAGGATTGGATGATGAATCCTTTGAGAATTACCAAGGTGGGTTAATAGCGAAGCTATTGGAGAAAGATTCAACCCTTACGGATGAAACTGCTCGATATTGGGATCATATTATCTGCAAAAGGTATAAGTTTGACAAATGGGAAAAGATAGCAGAAGAACTCAGAAGTCTACAGAAGGGGGATGTTATCAACTTTTACAAGACTTACTTGCAATTATCATCTCCCAAGCGTCGGAGACTTGTAACTCGTGTTTGGGGTTGCAACACTGACATGAAAGAAGCTGAAGAAGCGCGAACGGAGTCTGAGCAAGTCATTGAAGACCTTGCAGCCTTTAAGAGGTCATCTCAGTTCTATGGTCATGGAAGTTGTCAGACAGTTGCTCCTCCGATTATGTGTAAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

617

Amino Acids

70.26

Weight (kDa)

4.78

Isoelectric Point (pI)

42.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 66 - 171 1e-29 Insulinase (Peptidase family M16)
Peptidase_M16_M PF16187 172 - 438 3.8e-77 Middle or third domain of peptidase_M16
PqqF-like_C_4 PF22456 460 - 517 3.6e-07 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 188, 431
AccI GTMKAC 2 cut(s) 865, 1618
AccII CGCG 2 cut(s) 945, 1741
AciI CCGC 4 cut(s) 537, 840, 923, 1110
AclWI GGATC 6 cut(s) 107, 743, 756, 1235, 1248, 1560
AcsI RAATTY 5 cut(s) 520, 794, 979, 1165, 1288
AcuI CTGAAG 2 cut(s) 1121, 1752
AfaI GTAC 6 cut(s) 65, 307, 896, 910, 1082, 1283
AfiI CCNNNNNNNGG 6 cut(s) 256, 692, 1015, 1368, 1680, 1785
AgsI TTSAA 6 cut(s) 149, 323, 1117, 1192, 1521, 1766
Alw26I GTCTC 5 cut(s) 48, 191, 610, 1301, 1678
AlwI GGATC 6 cut(s) 107, 743, 756, 1235, 1248, 1560
Ama87I CYCGRG 1 cut(s) 1361
AoxI GGCC 3 cut(s) 88, 134, 875
ApaI GGGCCC 1 cut(s) 138
ApeKI GCWGC 8 cut(s) 9, 170, 209, 212, 215, 237, 539, 1775
ApoI RAATTY 5 cut(s) 520, 794, 979, 1165, 1288
Asp700I GAANNNNTTC 2 cut(s) 798, 1352
AspLEI GCGC 2 cut(s) 544, 1741
AspS9I GGNCC 4 cut(s) 134, 135, 875, 1371
AsuHPI GGTGA 2 cut(s) 456, 895
AvaI CYCGRG 1 cut(s) 1361
AvaII GGWCC 1 cut(s) 1371
BaeGI GKGCMC 1 cut(s) 138
BaeI ACNNNNGTAYC 2 cut(s) 1056, 1089
BamHI GGATCC 2 cut(s) 748, 1240
BanI GGYRCC 2 cut(s) 188, 431
BanII GRGCYC 1 cut(s) 138
BarI GAAGNNNNNNTAC 2 cut(s) 878, 910
BauI CACGAG 1 cut(s) 1695
BbsI GAAGAC 1 cut(s) 1773
BbvI GCAGC 7 cut(s) 182, 221, 224, 227, 249, 551, 1787
BccI CCATC 4 cut(s) 179, 354, 824, 866
BceAI ACGGC 1 cut(s) 222
BcoDI GTCTC 5 cut(s) 48, 191, 610, 1301, 1678
BfaI CTAG 5 cut(s) 281, 569, 605, 1047, 1451
BfmI CTRYAG 2 cut(s) 507, 1619
BisI GCNGC 8 cut(s) 10, 171, 210, 213, 216, 238, 540, 1776
BlsI GCNGC 8 cut(s) 11, 172, 211, 214, 217, 239, 541, 1777
BmcAI AGTACT 1 cut(s) 910
Bme18I GGWCC 1 cut(s) 1371
BmeT110I CYCGRG 1 cut(s) 1361
BmgT120I GGNCC 4 cut(s) 134, 135, 875, 1371
BmiI GGNNCC 7 cut(s) 136, 190, 433, 750, 1242, 1250, 1373
BmrI ACTGGG 1 cut(s) 147
BmsI GCATC 2 cut(s) 784, 971
BmuI ACTGGG 1 cut(s) 147
BpiI GAAGAC 1 cut(s) 1773
BplI GAGNNNNNCTC 2 cut(s) 1777, 1809
BpmI CTGGAG 1 cut(s) 1115
BpuEI CTTGAG 4 cut(s) 101, 634, 738, 1285
BsaBI GATNNNNATC 1 cut(s) 1389
BsaI GGTCTC 1 cut(s) 610
BsaJI CCNNGG 6 cut(s) 21, 486, 686, 708, 1244, 1483
BsaXI ACNNNNNCTCC 4 cut(s) 482, 512, 1813, 1843
Bsc4I CCNNNNNNNGG 6 cut(s) 256, 692, 1015, 1368, 1680, 1785
Bse1I ACTGG 2 cut(s) 153, 676
Bse3DI GCAATG 3 cut(s) 98, 225, 770
Bse8I GATNNNNATC 1 cut(s) 1389
BseDI CCNNGG 6 cut(s) 21, 486, 686, 708, 1244, 1483
BseGI GGATG 7 cut(s) 365, 586, 816, 1024, 1465, 1537, 1636
BseJI GATNNNNATC 1 cut(s) 1389
BseLI CCNNNNNNNGG 6 cut(s) 256, 692, 1015, 1368, 1680, 1785
BseMI GCAATG 3 cut(s) 98, 225, 770
BseMII CTCAG 6 cut(s) 108, 186, 464, 1624, 1743, 1808
BseNI ACTGG 2 cut(s) 153, 676
BseRI GAGGAG 2 cut(s) 10, 1821
BseSI GKGCMC 1 cut(s) 138
BseXI GCAGC 7 cut(s) 182, 221, 224, 227, 249, 551, 1787
BseYI CCCAGC 2 cut(s) 1056, 1060
Bsh1236I CGCG 2 cut(s) 945, 1741
BshFI GGCC 3 cut(s) 90, 136, 877
BshNI GGYRCC 2 cut(s) 188, 431
BsiHKCI CYCGRG 1 cut(s) 1361
BslFI GGGAC 2 cut(s) 572, 1357
BslI CCNNNNNNNGG 6 cut(s) 256, 692, 1015, 1368, 1680, 1785
BsmAI GTCTC 5 cut(s) 48, 191, 610, 1301, 1678
BsmBI CGTCTC 1 cut(s) 1301
BsmFI GGGAC 2 cut(s) 572, 1357
BsnI GGCC 3 cut(s) 90, 136, 877
Bso31I GGTCTC 1 cut(s) 610
BsoBI CYCGRG 1 cut(s) 1361
Bsp120I GGGCCC 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 138
Bsp1407I TGTACA 2 cut(s) 63, 1080
Bsp143I GATC 4 cut(s) 112, 748, 1240, 1552
Bsp19I CCATGG 1 cut(s) 486
BspACI CCGC 4 cut(s) 537, 840, 923, 1110
BspANI GGCC 3 cut(s) 90, 136, 877
BspCNI CTCAG 6 cut(s) 109, 187, 463, 1623, 1744, 1807
BspFNI CGCG 2 cut(s) 945, 1741
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 7 cut(s) 136, 190, 433, 750, 1242, 1250, 1373
BspPI GGATC 6 cut(s) 107, 743, 756, 1235, 1248, 1560
BspQI GCTCTTC 1 cut(s) 1434
BspT107I GGYRCC 2 cut(s) 188, 431
BspTNI GGTCTC 1 cut(s) 610
BsrDI GCAATG 3 cut(s) 98, 225, 770
BsrGI TGTACA 2 cut(s) 63, 1080
BsrI ACTGG 2 cut(s) 153, 676
BssECI CCNNGG 6 cut(s) 21, 486, 686, 708, 1244, 1483
BssMI GATC 4 cut(s) 112, 748, 1240, 1552
BssNAI GTATAC 1 cut(s) 866
BssSI CACGAG 1 cut(s) 1695
BssT1I CCWWGG 4 cut(s) 486, 686, 1244, 1483
Bst1107I GTATAC 1 cut(s) 866
Bst2BI CACGAG 1 cut(s) 1695
Bst4CI ACNGT 2 cut(s) 1336, 1825
Bst6I CTCTTC 1 cut(s) 1434
BstAPI GCANNNNNTGC 1 cut(s) 545
BstAUI TGTACA 2 cut(s) 63, 1080
BstC8I GCNNGC 5 cut(s) 270, 546, 683, 927, 1218
BstDEI CTNAG 8 cut(s) 117, 195, 201, 450, 648, 1610, 1752, 1794
BstDSI CCRYGG 1 cut(s) 486
BstENI CCTNNNNNAGG 1 cut(s) 1783
BstF5I GGATG 7 cut(s) 365, 586, 816, 1024, 1465, 1537, 1636
BstFNI CGCG 2 cut(s) 945, 1741
BstHHI GCGC 2 cut(s) 544, 1741
BstKTI GATC 4 cut(s) 115, 751, 1243, 1555
BstMAI GTCTC 5 cut(s) 48, 191, 610, 1301, 1678
BstMBI GATC 4 cut(s) 112, 748, 1240, 1552
BstMWI GCNNNNNNNGC 4 cut(s) 215, 545, 1052, 1736
BstNSI RCATGY 4 cut(s) 244, 292, 548, 1319
BstSFI CTRYAG 2 cut(s) 507, 1619
BstSLI GKGCMC 1 cut(s) 138
BstUI CGCG 2 cut(s) 945, 1741
BstV1I GCAGC 7 cut(s) 182, 221, 224, 227, 249, 551, 1787
BstV2I GAAGAC 1 cut(s) 1773
BstX2I RGATCY 2 cut(s) 748, 1240
BstYI RGATCY 2 cut(s) 748, 1240
BstZ17I GTATAC 1 cut(s) 866
BsuRI GGCC 3 cut(s) 90, 136, 877
BtgI CCRYGG 1 cut(s) 486
BtsCI GGATG 7 cut(s) 365, 586, 816, 1024, 1465, 1537, 1636
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 5 cut(s) 93, 160, 683, 1300, 1713
Cac8I GCNNGC 5 cut(s) 270, 546, 683, 927, 1218
CciI TCATGA 1 cut(s) 109
CfoI GCGC 2 cut(s) 544, 1741
Cfr13I GGNCC 4 cut(s) 134, 135, 875, 1371
CseI GACGC 2 cut(s) 934, 1667
Csp6I GTAC 6 cut(s) 64, 306, 895, 909, 1081, 1282
CviQI GTAC 6 cut(s) 64, 306, 895, 909, 1081, 1282
DdeI CTNAG 8 cut(s) 117, 195, 201, 450, 648, 1610, 1752, 1794
DpnI GATC 4 cut(s) 114, 750, 1242, 1554
DpnII GATC 4 cut(s) 112, 748, 1240, 1552
DraI TTTAAA 1 cut(s) 1323
Eam1104I CTCTTC 1 cut(s) 1434
EarI CTCTTC 1 cut(s) 1434
Eco130I CCWWGG 4 cut(s) 486, 686, 1244, 1483
Eco24I GRGCYC 1 cut(s) 138
Eco31I GGTCTC 1 cut(s) 610
Eco32I GATATC 1 cut(s) 645
Eco47I GGWCC 1 cut(s) 1371
Eco57I CTGAAG 2 cut(s) 1121, 1752
Eco88I CYCGRG 1 cut(s) 1361
EcoNI CCTNNNNNAGG 1 cut(s) 1783
EcoO109I RGGNCCY 1 cut(s) 134
EcoRI GAATTC 2 cut(s) 794, 979
EcoRV GATATC 1 cut(s) 645
EcoT14I CCWWGG 4 cut(s) 486, 686, 1244, 1483
EcoT22I ATGCAT 1 cut(s) 373
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 4 cut(s) 486, 686, 1244, 1483
Esp3I CGTCTC 1 cut(s) 1301
FalI AAGNNNNNCTT 4 cut(s) 419, 451, 1640, 1672
FaqI GGGAC 2 cut(s) 572, 1357
FauI CCCGC 1 cut(s) 544
FblI GTMKAC 2 cut(s) 865, 1618
Fnu4HI GCNGC 8 cut(s) 10, 171, 210, 213, 216, 238, 540, 1776
FokI GGATG 7 cut(s) 372, 593, 803, 1031, 1472, 1544, 1643
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 8 cut(s) 10, 171, 210, 213, 216, 238, 540, 1776
FspBI CTAG 5 cut(s) 281, 569, 605, 1047, 1451
GlaI GCGC 2 cut(s) 543, 1740
GluI GCNGC 8 cut(s) 10, 171, 210, 213, 216, 238, 540, 1776
GsaI CCCAGC 2 cut(s) 1060, 1064
GsuI CTGGAG 1 cut(s) 1115
HaeIII GGCC 3 cut(s) 90, 136, 877
HgaI GACGC 2 cut(s) 934, 1667
HhaI GCGC 2 cut(s) 544, 1741
Hin6I GCGC 2 cut(s) 542, 1739
HinP1I GCGC 2 cut(s) 542, 1739
HincII GTYRAC 1 cut(s) 1417
HindII GTYRAC 1 cut(s) 1417
HindIII AAGCTT 2 cut(s) 899, 1847
HinfI GANTC 6 cut(s) 512, 991, 1126, 1466, 1517, 1748
HphI GGTGA 2 cut(s) 456, 895
Hpy166II GTNNAC 5 cut(s) 866, 1081, 1083, 1417, 1619
Hpy8I GTNNAC 5 cut(s) 866, 1081, 1083, 1417, 1619
Hpy99I CGWCG 2 cut(s) 1381, 1683
HpyAV CCTTC 4 cut(s) 739, 1019, 1096, 1618
HpyCH4III ACNGT 2 cut(s) 1336, 1825
HpyCH4IV ACGT 2 cut(s) 659, 1310
HpyF10VI GCNNNNNNNGC 4 cut(s) 215, 545, 1052, 1736
HpyF3I CTNAG 8 cut(s) 117, 195, 201, 450, 648, 1610, 1752, 1794
HpySE526I ACGT 2 cut(s) 659, 1310
HspAI GCGC 2 cut(s) 542, 1739
Kzo9I GATC 4 cut(s) 112, 748, 1240, 1552
LguI GCTCTTC 1 cut(s) 1434
LmnI GCTCC 5 cut(s) 490, 1096, 1213, 1248, 1834
Lsp1109I GCAGC 7 cut(s) 182, 221, 224, 227, 249, 551, 1787
LweI GCATC 2 cut(s) 784, 971
MaeI CTAG 5 cut(s) 281, 569, 605, 1047, 1451
MaeII ACGT 2 cut(s) 659, 1310
MaeIII GTNAC 4 cut(s) 338, 498, 696, 1690
MalI GATC 4 cut(s) 114, 750, 1242, 1554
MboI GATC 4 cut(s) 112, 748, 1240, 1552
MboII GAAGA 9 cut(s) 170, 173, 680, 725, 988, 1451, 1616, 1745, 1778
MflI RGATCY 2 cut(s) 748, 1240
MhlI GDGCHC 1 cut(s) 138
MlyI GAGTC 2 cut(s) 985, 1757
MmeI TCCRAC 3 cut(s) 208, 1154, 1661
Mph1103I ATGCAT 1 cut(s) 373
MroXI GAANNNNTTC 2 cut(s) 798, 1352
MseI TTAA 4 cut(s) 1200, 1322, 1493, 1782
MslI CAYNNNNRTG 2 cut(s) 1235, 1718
MspA1I CMGCKG 2 cut(s) 1060, 1110
MvnI CGCG 2 cut(s) 945, 1741
MwoI GCNNNNNNNGC 4 cut(s) 215, 545, 1052, 1736
NcoI CCATGG 1 cut(s) 486
NdeII GATC 4 cut(s) 112, 748, 1240, 1552
NlaIV GGNNCC 7 cut(s) 136, 190, 433, 750, 1242, 1250, 1373
NmuCI GTSAC 1 cut(s) 696
NsiI ATGCAT 1 cut(s) 373
NspI RCATGY 4 cut(s) 244, 292, 548, 1319
PaeI GCATGC 1 cut(s) 548
PagI TCATGA 1 cut(s) 109
PciSI GCTCTTC 1 cut(s) 1434
PdmI GAANNNNTTC 2 cut(s) 798, 1352
PfeI GAWTC 4 cut(s) 512, 1126, 1466, 1517
PkrI GCNGC 8 cut(s) 11, 172, 211, 214, 217, 239, 541, 1777
PleI GAGTC 2 cut(s) 985, 1756
PpsI GAGTC 2 cut(s) 985, 1756
PspFI CCCAGC 2 cut(s) 1056, 1060
PspN4I GGNNCC 7 cut(s) 136, 190, 433, 750, 1242, 1250, 1373
PspOMI GGGCCC 1 cut(s) 134
PspPI GGNCC 4 cut(s) 134, 135, 875, 1371
PsuI RGATCY 2 cut(s) 748, 1240
PvuII CAGCTG 1 cut(s) 1060
RsaI GTAC 6 cut(s) 65, 307, 896, 910, 1082, 1283
RsaNI GTAC 6 cut(s) 64, 306, 895, 909, 1081, 1282
RseI CAYNNNNRTG 2 cut(s) 1235, 1718
SapI GCTCTTC 1 cut(s) 1434
SaqAI TTAA 4 cut(s) 1200, 1322, 1493, 1782
SatI GCNGC 8 cut(s) 10, 171, 210, 213, 216, 238, 540, 1776
Sau3AI GATC 4 cut(s) 112, 748, 1240, 1552
Sau96I GGNCC 4 cut(s) 134, 135, 875, 1371
ScaI AGTACT 1 cut(s) 910
SchI GAGTC 2 cut(s) 985, 1757
SduI GDGCHC 1 cut(s) 138
SfaNI GCATC 2 cut(s) 784, 971
SfcI CTRYAG 2 cut(s) 507, 1619
SinI GGWCC 1 cut(s) 1371
SmiMI CAYNNNNRTG 2 cut(s) 1235, 1718
SmlI CTYRAG 4 cut(s) 80, 613, 753, 1264
SmoI CTYRAG 4 cut(s) 80, 613, 753, 1264
SphI GCATGC 1 cut(s) 548
SsiI CCGC 4 cut(s) 537, 840, 923, 1110
SspMI CTAG 5 cut(s) 281, 569, 605, 1047, 1451
StyI CCWWGG 4 cut(s) 486, 686, 1244, 1483
TaaI ACNGT 2 cut(s) 1336, 1825
TaiI ACGT 2 cut(s) 662, 1313
TaqI TCGA 2 cut(s) 1256, 1543
TatI WGTACW 4 cut(s) 63, 305, 908, 1080
TfiI GAWTC 4 cut(s) 512, 1126, 1466, 1517
Tru1I TTAA 4 cut(s) 1200, 1322, 1493, 1782
Tru9I TTAA 4 cut(s) 1200, 1322, 1493, 1782
TscAI CASTG 5 cut(s) 100, 160, 683, 1300, 1720
TseFI GTSAC 1 cut(s) 696
TseI GCWGC 8 cut(s) 9, 170, 209, 212, 215, 237, 539, 1775
Tsp45I GTSAC 1 cut(s) 696
TspGWI ACGGA 4 cut(s) 714, 809, 1544, 1760
TspRI CASTG 5 cut(s) 100, 160, 683, 1300, 1720
VpaK11BI GGWCC 1 cut(s) 1371
XagI CCTNNNNNAGG 1 cut(s) 1783
XapI RAATTY 5 cut(s) 520, 794, 979, 1165, 1288
XceI RCATGY 4 cut(s) 244, 292, 548, 1319
XmiI GTMKAC 2 cut(s) 865, 1618
XmnI GAANNNNTTC 2 cut(s) 798, 1352
XspI CTAG 5 cut(s) 281, 569, 605, 1047, 1451
ZrmI AGTACT 1 cut(s) 910
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.