Rh5DG510400

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000183
Physical Location & Seq
Reverse (-)
79726913 .. 79739059
12147 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG510400.1

Sequence Viewer

Length: 2748 bp
ATGCGCATACCCTTCTTTATAGGGTGTGAACACATTACTTACAAACTAAATGTTGCAGAACACATGCTGTTCATGGGGAGTACAAAATTCCCAGTTGAAAATGAGTATGATAGTTACTTGTCCAAGCATGGAGGGTCATCAAATGCATACACCTGTGCAGAGCATACTTGCTACTTTTTTGAAGTGAAACCGGAGTTTCTTAAGGGTGCCTTGACAAGATTTTCTCAGTTCTTTGTTTCACCTCTGATAAAAAGTGAATCCATGGAGCGAGAGATACAGGCTATAGATTCAGAGTTTAACAGGGTTCTGCAAAATGACTTTTGCCGCCTTCGACAACTTCAATGCTATACATCCACACCAGGTCACCCGTTTAATAGATTTTCTCGAGGAAATAAGAAGAGCTTGGATGATGCAACCAAAAAGGGGATCAACTTGCAGGAACAAATACTGAAATTGTACAGAAACTATTACCGCGGTGGATTAATGAAGCTAGTTGTCATTGGTGGAGAGTCGCTCGATGTACTAGAGAAGTGGAGTTTGGAATTGTATAGAGATGTCAAAACCGGTCCCCAAGTAAATCTGGAGTTCAAGGCAGACGGTCCTATTTGGAAAGCTGGAAAAGTTTACAGGCTAGAGGCTGTTCAAGATGTTCATATACTGCAATTGTCATGGAAACTTCCATGCCTTCTTCAACACTATTTGAAAGGCCCAGAATTTTATTTACATCATCTCCTTGTGCATGAGGGCAGGGGAAGTTTGCATTTCTACCTCAAAGCTAGAGGGTGGGCAACGTATCTAGATGCTTCGGTGAGCCGCTATCCTGTGGCCGATGTCTTTTGCATGATCATATACCTCACTGACTCTGGACTGGAGAAGATTTTTGAAATAATTGGGTTGGTGTATCAATACATTAAGTTACTACGTCAGGAGACGCCAGAATGGATATTTCGGGAACTCCAGGATATAGGGAACATGTGCTTTAGATTTGCAGAGGAGGAGCCTTTCCAGGATGATTATGTTTCAAACCTTGCAGAAAATTTACGACGTTATGCAGCAGAGCATGTTATTTATGGGGACTATGTGTTTGGGATATGGGATGAGGTATTGATACAAAATGTTCTGGGTTTCTTCTCTCCAGAAAACATGAGGATTGATGTGGTATCAAAGTTCTCATTTAAGTCAGAAGAGTTTCAGTGCGAGCCTTGGTTCGGGTCACATTATACCGAGGAAGATATATCTCCAGCTTTGATGGAATTGTGGAAGGATCCTCCCGAAATTGATGTATCGTTGCATCTGCGGGCAAAAAATGAGTTCATTCCTAGTGACTTTTCCATCCGTTCTGATGGGATGTGTCTTGATATTTCAAATATATCTTCTCCGGCATGTATGTTTGATGAACCATTGATGAAGATCTGGTACAAGCTTGATAGTACTTTTAAACTTCCACGGGCAAATGCGTACTTCTTCATCAGAATGAAAGGTGGATATGATAATGTAAAAAGTAGTGTTTTGACAGACTTATACATCGACCTTCTTCAAGATGAGCTCAGTGAGATCATCTATCAGGCTTATGTTACCTGCTTGGGGACTTCTATTTCTGTGTTTACCGACAAACTCCAGCTGCAGGTGTATGGTTTCAATGATAAGCTTCCAGCTCTGTTGTCAAAAATTGTGGAAACAGTAAAGAGCTTCTTGCCAACTGATGATCGTTTTGAGGTTATTAAAGAAGACATGGTGCGAGTGTATAGGAATGCCAATATGAATCCTTGGGGTCACTCAACATACTTGAGAGAGCAAGTTTTGTTGCAGAGTTTCTACAACGTAGATGAGCTGTTTCATGTTTTGAATGGGTTGTCCGTTTCTGATTTGAAGTCATTTATTCCTGAGCTTTTCTCCCAGATCTACATTGAGGGCCTTTTCCATGGTAATTTGTCAGAAGAAGAAGCAATTAGCCTCTCAAATTTATTCAAGACAATCTTTTCTGTAAAACCACTTCCTGTTGAATTGATGTATAGAAATCATTGTATTTGTCTTCCTTCTAATGCTAACCTCATTAGAGATGCTACTGTGAAGAACAAGTCAGAAACAAATTCTGTGACAGAGCTGTATTTTCAAATTGAGTGGGCAGTAGGGAGCGAGTCCGTGAGATGGAAAGTTTTGATAGATCTTTTCGATGCAATTGTGAAGGAACCACTTTTTAATCAGCTAAGGACAAAGGAGCAGCTCGGGTATGGAGTTCAGTGTGGTCGGGATTTAAAATGGGGTGTTTTTGGCATTCTTTTCTCTGTTCAGTCATCTGAGTACAACCCAATCCACCTACAAGGAAGACTAGACAACTTCATAAATGGTCTGGAAGATTTGTTGGAAGGACTGGATGATGATTCCTTTGAGAATTATAAAGCTGGACTAATGGAAAATCTTTTGAAGAAAGATACATCCCTCACGTGTGAAACCAATCGATTGTGGAATGAGATTTATATGAAAAGGTATATGTTTGACTACTCAAAAAAGGCAGCAGAAGAACTCAGAAGCATTCAGAAGGGGGATGTTATCAACTTTTACAAGACATATTTGCAACAATCATCTCCCAAGTGTCGAAGACTTGCAACTCGTGTTTGGGGTTGCAACAACGACTTCAAAGAGGCAGAAGTAGCACGACTGGAGTCTGTGCAAGTCATTGAAGACGTTGCAGCCTTTAAGATGTCATCGAAGTTCTATAAACCTAAGATAAATACTCTGGATACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

915

Amino Acids

106.33

Weight (kDa)

5.46

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 19 - 105 4.2e-19 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 147 - 320 3.5e-15 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 327 - 612 4.3e-80 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 618 - 803 1.9e-17 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 724 - 822 2.4e-22 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2397
AarI CACCTGC 1 cut(s) 1615
Acc16I TGCGCA 1 cut(s) 5
Acc36I ACCTGC 2 cut(s) 1586, 1615
AccB1I GGYRCC 1 cut(s) 206
AccII CGCG 1 cut(s) 474
AciI CCGC 5 cut(s) 325, 472, 474, 814, 1297
AclWI GGATC 3 cut(s) 434, 1259, 1272
AcoI YGGCCR 1 cut(s) 825
AcsI RAATTY 5 cut(s) 86, 713, 1036, 1960, 2089
AcvI CACGTG 1 cut(s) 2445
AcyI GRCGYC 1 cut(s) 932
AfaI GTAC 7 cut(s) 82, 458, 522, 1418, 1432, 1460, 2303
AfiI CCNNNNNNNGG 5 cut(s) 423, 1208, 1584, 1624, 2148
AflII CTTAAG 1 cut(s) 200
AflIII ACRYGT 2 cut(s) 972, 2444
AgeI ACCGGT 1 cut(s) 563
AjnI CCWGG 3 cut(s) 358, 957, 1005
AjuI GAANNNNNNNTTGG 2 cut(s) 521, 553
Alw21I GWGCWC 1 cut(s) 1548
Alw26I GTCTC 1 cut(s) 923
AlwI GGATC 3 cut(s) 434, 1259, 1272
Ama87I CYCGRG 2 cut(s) 384, 2225
AoxI GGCC 3 cut(s) 706, 825, 1912
ApeKI GCWGC 5 cut(s) 1052, 1621, 2221, 2513, 2690
ApoI RAATTY 5 cut(s) 86, 713, 1036, 1960, 2089
ArsI GACNNNNNNTTYG 2 cut(s) 1067, 1099
AseI ATTAAT 1 cut(s) 482
AsiGI ACCGGT 1 cut(s) 563
Asp700I GAANNNNTTC 1 cut(s) 1188
AspLEI GCGC 1 cut(s) 6
AspS9I GGNCC 4 cut(s) 566, 599, 707, 1912
AsuHPI GGTGA 3 cut(s) 231, 356, 820
AvaI CYCGRG 2 cut(s) 384, 2225
AvaII GGWCC 2 cut(s) 566, 599
BamHI GGATCC 1 cut(s) 1264
BanI GGYRCC 1 cut(s) 206
BanII GRGCYC 1 cut(s) 1548
BarI GAAGNNNNNNTAC 4 cut(s) 1400, 1432, 1977, 2009
BauI CACGAG 1 cut(s) 2610
BbrPI CACGTG 1 cut(s) 2445
BbsI GAAGAC 5 cut(s) 1734, 2024, 2332, 2605, 2688
Bbv12I GWGCWC 1 cut(s) 1548
BbvI GCAGC 5 cut(s) 1064, 1608, 2233, 2525, 2702
BccI CCATC 4 cut(s) 1243, 1337, 1340, 2142
BciT130I CCWGG 3 cut(s) 360, 959, 1007
BciVI GTATCC 1 cut(s) 2734
BclI TGATCA 1 cut(s) 843
BcoDI GTCTC 1 cut(s) 923
BfaI CTAG 7 cut(s) 491, 524, 632, 777, 797, 1320, 2330
BfmI CTRYAG 2 cut(s) 282, 1622
BfrI CTTAAG 1 cut(s) 200
BfuAI ACCTGC 2 cut(s) 1586, 1615
BfuI GTATCC 1 cut(s) 2734
BglII AGATCT 3 cut(s) 1410, 1899, 2164
BisI GCNGC 7 cut(s) 325, 814, 1053, 1622, 2222, 2514, 2691
BlsI GCNGC 7 cut(s) 326, 815, 1054, 1623, 2223, 2515, 2692
BmcAI AGTACT 1 cut(s) 1432
Bme1390I CCNGG 3 cut(s) 360, 959, 1007
Bme18I GGWCC 2 cut(s) 566, 599
BmeT110I CYCGRG 2 cut(s) 384, 2225
BmgT120I GGNCC 4 cut(s) 566, 599, 707, 1912
BmiI GGNNCC 5 cut(s) 208, 568, 999, 1266, 2190
BmrFI CCNGG 3 cut(s) 360, 959, 1007
BmrI ACTGGG 1 cut(s) 86
BmsI GCATC 5 cut(s) 400, 790, 1300, 2050, 2164
BmuI ACTGGG 1 cut(s) 86
BoxI GACNNNNGTC 1 cut(s) 2662
BpiI GAAGAC 5 cut(s) 1734, 2024, 2332, 2605, 2688
BplI GAGNNNNNCTC 4 cut(s) 498, 530, 1877, 1909
BpmI CTGGAG 7 cut(s) 602, 890, 941, 1119, 1224, 1601, 2681
Bpu10I CCTNAGC 2 cut(s) 1884, 2207
BpuEI CTTGAG 1 cut(s) 1807
Bsa29I ATCGAT 1 cut(s) 2458
BsaAI YACGTR 1 cut(s) 2445
BsaBI GATNNNNATC 1 cut(s) 1409
BsaHI GRCGYC 1 cut(s) 932
BsaJI CCNNGG 7 cut(s) 261, 472, 1202, 1224, 1445, 1766, 1921
BsaWI WCCGGW 2 cut(s) 190, 563
BsaXI ACNNNNNCTCC 2 cut(s) 714, 744
Bsc4I CCNNNNNNNGG 5 cut(s) 423, 1208, 1584, 1624, 2148
Bse118I RCCGGY 1 cut(s) 563
Bse1I ACTGG 4 cut(s) 92, 873, 2376, 2664
Bse8I GATNNNNATC 1 cut(s) 1409
BseBI CCWGG 3 cut(s) 360, 959, 1007
BseCI ATCGAT 1 cut(s) 2458
BseDI CCNNGG 7 cut(s) 261, 472, 1202, 1224, 1445, 1766, 1921
BseGI GGATG 9 cut(s) 350, 412, 1015, 1102, 1332, 1353, 2380, 2435, 2551
BseJI GATNNNNATC 1 cut(s) 1409
BseLI CCNNNNNNNGG 5 cut(s) 423, 1208, 1584, 1624, 2148
BseMII CTCAG 5 cut(s) 239, 1561, 1875, 2289, 2539
BseNI ACTGG 4 cut(s) 92, 873, 2376, 2664
BseRI GAGGAG 2 cut(s) 1007, 1010
BseXI GCAGC 5 cut(s) 1064, 1608, 2233, 2525, 2702
BsgI GTGCAG 1 cut(s) 177
Bsh1236I CGCG 1 cut(s) 474
BshFI GGCC 3 cut(s) 708, 827, 1914
BshNI GGYRCC 1 cut(s) 206
BshTI ACCGGT 1 cut(s) 563
BshVI ATCGAT 1 cut(s) 2458
BsiHKAI GWGCWC 1 cut(s) 1548
BsiHKCI CYCGRG 2 cut(s) 384, 2225
BsiSI CCGG 3 cut(s) 191, 564, 1379
BslFI GGGAC 3 cut(s) 552, 1088, 1600
BslI CCNNNNNNNGG 5 cut(s) 423, 1208, 1584, 1624, 2148
BsmAI GTCTC 1 cut(s) 923
BsmBI CGTCTC 1 cut(s) 923
BsmFI GGGAC 3 cut(s) 552, 1088, 1600
BsmI GAATGC 3 cut(s) 1756, 2274, 2532
BsnI GGCC 3 cut(s) 708, 827, 1914
BsoBI CYCGRG 2 cut(s) 384, 2225
Bsp1286I GDGCHC 1 cut(s) 1548
Bsp1407I TGTACA 1 cut(s) 456
Bsp143I GATC 8 cut(s) 426, 843, 1264, 1410, 1554, 1705, 1899, 2164
Bsp19I CCATGG 2 cut(s) 261, 1921
BspACI CCGC 5 cut(s) 325, 472, 474, 814, 1297
BspANI GGCC 3 cut(s) 708, 827, 1914
BspCNI CTCAG 5 cut(s) 238, 1560, 1876, 2290, 2538
BspDI ATCGAT 1 cut(s) 2458
BspFNI CGCG 1 cut(s) 474
BspLI GGNNCC 5 cut(s) 208, 568, 999, 1266, 2190
BspMAI CTGCAG 1 cut(s) 1626
BspMI ACCTGC 2 cut(s) 1586, 1615
BspPI GGATC 3 cut(s) 434, 1259, 1272
BspQI GCTCTTC 1 cut(s) 392
BspT107I GGYRCC 1 cut(s) 206
BspTI CTTAAG 1 cut(s) 200
BsrFI RCCGGY 1 cut(s) 563
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 4 cut(s) 92, 873, 2376, 2664
BssAI RCCGGY 1 cut(s) 563
BssECI CCNNGG 7 cut(s) 261, 472, 1202, 1224, 1445, 1766, 1921
BssMI GATC 8 cut(s) 426, 843, 1264, 1410, 1554, 1705, 1899, 2164
BssNI GRCGYC 1 cut(s) 932
BssSI CACGAG 1 cut(s) 2610
BssT1I CCWWGG 4 cut(s) 261, 1202, 1766, 1921
Bst2BI CACGAG 1 cut(s) 2610
Bst2UI CCWGG 3 cut(s) 360, 959, 1007
Bst4CI ACNGT 3 cut(s) 599, 1681, 2068
Bst6I CTCTTC 2 cut(s) 392, 1179
BstACI GRCGYC 1 cut(s) 932
BstAFI CTTAAG 1 cut(s) 200
BstAUI TGTACA 1 cut(s) 456
BstBAI YACGTR 1 cut(s) 2445
BstC8I GCNNGC 2 cut(s) 1199, 1299
BstDEI CTNAG 7 cut(s) 225, 1547, 1884, 2207, 2298, 2525, 2724
BstDSI CCRYGG 4 cut(s) 261, 472, 1445, 1921
BstEII GGTNACC 1 cut(s) 362
BstF5I GGATG 9 cut(s) 350, 412, 1015, 1102, 1332, 1353, 2380, 2435, 2551
BstFNI CGCG 1 cut(s) 474
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 8 cut(s) 429, 846, 1267, 1413, 1557, 1708, 1902, 2167
BstMAI GTCTC 1 cut(s) 923
BstMBI GATC 8 cut(s) 426, 843, 1264, 1410, 1554, 1705, 1899, 2164
BstMWI GCNNNNNNNGC 1 cut(s) 2651
BstNI CCWGG 3 cut(s) 360, 959, 1007
BstNSI RCATGY 4 cut(s) 67, 976, 1064, 1386
BstPAI GACNNNNGTC 1 cut(s) 2662
BstPI GGTNACC 1 cut(s) 362
BstSCI CCNGG 3 cut(s) 358, 957, 1005
BstSFI CTRYAG 2 cut(s) 282, 1622
BstUI CGCG 1 cut(s) 474
BstV1I GCAGC 5 cut(s) 1064, 1608, 2233, 2525, 2702
BstV2I GAAGAC 5 cut(s) 1734, 2024, 2332, 2605, 2688
BstX2I RGATCY 4 cut(s) 1264, 1410, 1899, 2164
BstYI RGATCY 4 cut(s) 1264, 1410, 1899, 2164
Bsu15I ATCGAT 1 cut(s) 2458
BsuI GTATCC 1 cut(s) 2734
BsuRI GGCC 3 cut(s) 708, 827, 1914
BsuTUI ATCGAT 1 cut(s) 2458
BtgI CCRYGG 4 cut(s) 261, 472, 1445, 1921
BtsCI GGATG 9 cut(s) 350, 412, 1015, 1102, 1332, 1353, 2380, 2435, 2551
BtsIMutI CAGTG 4 cut(s) 855, 1199, 1555, 2246
BveI ACCTGC 2 cut(s) 1586, 1615
Cac8I GCNNGC 2 cut(s) 1199, 1299
CfoI GCGC 1 cut(s) 6
Cfr10I RCCGGY 1 cut(s) 563
Cfr13I GGNCC 4 cut(s) 566, 599, 707, 1912
Cfr42I CCGCGG 1 cut(s) 475
ClaI ATCGAT 1 cut(s) 2458
CseI GACGC 1 cut(s) 940
CsiI ACCWGGT 1 cut(s) 358
Csp6I GTAC 7 cut(s) 81, 457, 521, 1417, 1431, 1459, 2302
CspAI ACCGGT 1 cut(s) 563
CspCI CAANNNNNGTGG 4 cut(s) 1653, 1688, 2102, 2137
CviQI GTAC 7 cut(s) 81, 457, 521, 1417, 1431, 1459, 2302
DdeI CTNAG 7 cut(s) 225, 1547, 1884, 2207, 2298, 2525, 2724
DpnI GATC 8 cut(s) 428, 845, 1266, 1412, 1556, 1707, 1901, 2166
DpnII GATC 8 cut(s) 426, 843, 1264, 1410, 1554, 1705, 1899, 2164
DraI TTTAAA 2 cut(s) 1438, 2256
EaeI YGGCCR 1 cut(s) 825
Eam1104I CTCTTC 2 cut(s) 392, 1179
EarI CTCTTC 2 cut(s) 392, 1179
Ecl136II GAGCTC 1 cut(s) 1546
Eco130I CCWWGG 4 cut(s) 261, 1202, 1766, 1921
Eco24I GRGCYC 1 cut(s) 1548
Eco47I GGWCC 2 cut(s) 566, 599
Eco53kI GAGCTC 1 cut(s) 1546
Eco72I CACGTG 1 cut(s) 2445
Eco88I CYCGRG 2 cut(s) 384, 2225
Eco91I GGTNACC 1 cut(s) 362
EcoICRI GAGCTC 1 cut(s) 1546
EcoO109I RGGNCCY 1 cut(s) 1912
EcoO65I GGTNACC 1 cut(s) 362
EcoRII CCWGG 3 cut(s) 358, 957, 1005
EcoT14I CCWWGG 4 cut(s) 261, 1202, 1766, 1921
EcoT22I ATGCAT 1 cut(s) 148
EcoT38I GRGCYC 1 cut(s) 1548
ErhI CCWWGG 4 cut(s) 261, 1202, 1766, 1921
Esp3I CGTCTC 1 cut(s) 923
FalI AAGNNNNNCTT 8 cut(s) 194, 226, 386, 418, 1676, 1708, 1961, 1993
FaqI GGGAC 3 cut(s) 552, 1088, 1600
FauI CCCGC 1 cut(s) 1290
FbaI TGATCA 1 cut(s) 843
Fnu4HI GCNGC 7 cut(s) 325, 814, 1053, 1622, 2222, 2514, 2691
FokI GGATG 9 cut(s) 337, 419, 1022, 1109, 1319, 1360, 2387, 2422, 2558
FriOI GRGCYC 1 cut(s) 1548
Fsp4HI GCNGC 7 cut(s) 325, 814, 1053, 1622, 2222, 2514, 2691
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 7 cut(s) 491, 524, 632, 777, 797, 1320, 2330
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 1 cut(s) 5
GluI GCNGC 7 cut(s) 325, 814, 1053, 1622, 2222, 2514, 2691
GsuI CTGGAG 7 cut(s) 602, 890, 941, 1119, 1224, 1601, 2681
HaeIII GGCC 3 cut(s) 708, 827, 1914
HapII CCGG 3 cut(s) 191, 564, 1379
HgaI GACGC 1 cut(s) 940
HhaI GCGC 1 cut(s) 6
Hin1I GRCGYC 1 cut(s) 932
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HindIII AAGCTT 2 cut(s) 1421, 1646
HinfI GANTC 8 cut(s) 257, 287, 509, 860, 1762, 2138, 2381, 2663
HpaII CCGG 3 cut(s) 191, 564, 1379
HphI GGTGA 3 cut(s) 231, 356, 820
Hpy166II GTNNAC 3 cut(s) 29, 625, 1605
Hpy8I GTNNAC 3 cut(s) 29, 625, 1605
Hpy99I CGWCG 1 cut(s) 1047
HpyAV CCTTC 9 cut(s) 22, 338, 695, 1255, 1541, 2046, 2179, 2360, 2533
HpyCH4III ACNGT 3 cut(s) 599, 1681, 2068
HpyCH4IV ACGT 6 cut(s) 791, 922, 1045, 1821, 2444, 2685
HpyF10VI GCNNNNNNNGC 1 cut(s) 2651
HpyF3I CTNAG 7 cut(s) 225, 1547, 1884, 2207, 2298, 2525, 2724
HpySE526I ACGT 6 cut(s) 791, 922, 1045, 1821, 2444, 2685
Hsp92I GRCGYC 1 cut(s) 932
HspAI GCGC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 843
KspI CCGCGG 1 cut(s) 475
Kzo9I GATC 8 cut(s) 426, 843, 1264, 1410, 1554, 1705, 1899, 2164
LguI GCTCTTC 1 cut(s) 392
LmnI GCTCC 4 cut(s) 265, 997, 2133, 2218
Lsp1109I GCAGC 5 cut(s) 1064, 1608, 2233, 2525, 2702
LweI GCATC 5 cut(s) 400, 790, 1300, 2050, 2164
MabI ACCWGGT 1 cut(s) 358
MaeI CTAG 7 cut(s) 491, 524, 632, 777, 797, 1320, 2330
MaeII ACGT 6 cut(s) 791, 922, 1045, 1821, 2444, 2685
MaeIII GTNAC 8 cut(s) 113, 362, 915, 1212, 1322, 1573, 1772, 2095
MalI GATC 8 cut(s) 428, 845, 1266, 1412, 1556, 1707, 1901, 2166
MboI GATC 8 cut(s) 426, 843, 1264, 1410, 1554, 1705, 1899, 2164
MfeI CAATTG 2 cut(s) 662, 2178
MflI RGATCY 4 cut(s) 1264, 1410, 1899, 2164
MhlI GDGCHC 1 cut(s) 1548
MlyI GAGTC 4 cut(s) 518, 854, 2147, 2672
MmeI TCCRAC 1 cut(s) 2343
Mph1103I ATGCAT 1 cut(s) 148
MroXI GAANNNNTTC 1 cut(s) 1188
MslI CAYNNNNRTG 1 cut(s) 1472
MspA1I CMGCKG 2 cut(s) 474, 1621
MspCI CTTAAG 1 cut(s) 200
MspI CCGG 3 cut(s) 191, 564, 1379
MspR9I CCNGG 3 cut(s) 360, 959, 1007
MunI CAATTG 2 cut(s) 662, 2178
Mva1269I GAATGC 3 cut(s) 1756, 2274, 2532
MvaI CCWGG 3 cut(s) 360, 959, 1007
MvnI CGCG 1 cut(s) 474
MwoI GCNNNNNNNGC 1 cut(s) 2651
NcoI CCATGG 2 cut(s) 261, 1921
NdeII GATC 8 cut(s) 426, 843, 1264, 1410, 1554, 1705, 1899, 2164
NlaIV GGNNCC 5 cut(s) 208, 568, 999, 1266, 2190
NmuCI GTSAC 5 cut(s) 362, 1212, 1322, 1772, 2095
NsbI TGCGCA 1 cut(s) 5
NsiI ATGCAT 1 cut(s) 148
NspI RCATGY 4 cut(s) 67, 976, 1064, 1386
PaeR7I CTCGAG 1 cut(s) 384
PaqCI CACCTGC 1 cut(s) 1615
PciI ACATGT 1 cut(s) 972
PciSI GCTCTTC 1 cut(s) 392
PctI GAATGC 3 cut(s) 1756, 2274, 2532
PdmI GAANNNNTTC 1 cut(s) 1188
PfeI GAWTC 4 cut(s) 257, 287, 1762, 2381
PfoI TCCNGGA 2 cut(s) 957, 1005
PinAI ACCGGT 1 cut(s) 563
PkrI GCNGC 7 cut(s) 326, 815, 1054, 1623, 2223, 2515, 2692
PleI GAGTC 4 cut(s) 517, 854, 2146, 2671
PmaCI CACGTG 1 cut(s) 2445
PmlI CACGTG 1 cut(s) 2445
PpsI GAGTC 4 cut(s) 517, 854, 2146, 2671
Ppu21I YACGTR 1 cut(s) 2445
PscI ACATGT 1 cut(s) 972
PshAI GACNNNNGTC 1 cut(s) 2662
PshBI ATTAAT 1 cut(s) 482
PsiI TTATAA 1 cut(s) 2397
Psp124BI GAGCTC 1 cut(s) 1548
Psp6I CCWGG 3 cut(s) 358, 957, 1005
PspCI CACGTG 1 cut(s) 2445
PspEI GGTNACC 1 cut(s) 362
PspGI CCWGG 3 cut(s) 358, 957, 1005
PspN4I GGNNCC 5 cut(s) 208, 568, 999, 1266, 2190
PspPI GGNCC 4 cut(s) 566, 599, 707, 1912
PstI CTGCAG 1 cut(s) 1626
PsuI RGATCY 4 cut(s) 1264, 1410, 1899, 2164
PvuII CAGCTG 1 cut(s) 1621
RsaI GTAC 7 cut(s) 82, 458, 522, 1418, 1432, 1460, 2303
RsaNI GTAC 7 cut(s) 81, 457, 521, 1417, 1431, 1459, 2302
RseI CAYNNNNRTG 1 cut(s) 1472
SacI GAGCTC 1 cut(s) 1548
SacII CCGCGG 1 cut(s) 475
SapI GCTCTTC 1 cut(s) 392
SatI GCNGC 7 cut(s) 325, 814, 1053, 1622, 2222, 2514, 2691
Sau3AI GATC 8 cut(s) 426, 843, 1264, 1410, 1554, 1705, 1899, 2164
Sau96I GGNCC 4 cut(s) 566, 599, 707, 1912
ScaI AGTACT 1 cut(s) 1432
SchI GAGTC 4 cut(s) 518, 854, 2147, 2672
ScrFI CCNGG 3 cut(s) 360, 959, 1007
SduI GDGCHC 1 cut(s) 1548
SexAI ACCWGGT 1 cut(s) 358
SfaNI GCATC 5 cut(s) 400, 790, 1300, 2050, 2164
SfcI CTRYAG 2 cut(s) 282, 1622
Sfr274I CTCGAG 1 cut(s) 384
Sfr303I CCGCGG 1 cut(s) 475
SgrBI CCGCGG 1 cut(s) 475
SinI GGWCC 2 cut(s) 566, 599
SlaI CTCGAG 1 cut(s) 384
SmiMI CAYNNNNRTG 1 cut(s) 1472
SmlI CTYRAG 3 cut(s) 200, 384, 1786
SmoI CTYRAG 3 cut(s) 200, 384, 1786
SsiI CCGC 5 cut(s) 325, 472, 474, 814, 1297
SspMI CTAG 7 cut(s) 491, 524, 632, 777, 797, 1320, 2330
SstI GAGCTC 1 cut(s) 1548
StyD4I CCNGG 3 cut(s) 358, 957, 1005
StyI CCWWGG 4 cut(s) 261, 1202, 1766, 1921
TaaI ACNGT 3 cut(s) 599, 1681, 2068
TaiI ACGT 6 cut(s) 794, 925, 1048, 1824, 2447, 2688
TaqI TCGA 8 cut(s) 331, 385, 516, 1527, 2172, 2458, 2596, 2708
TatI WGTACW 5 cut(s) 80, 456, 520, 1430, 2301
TauI GCSGC 2 cut(s) 327, 816
TfiI GAWTC 4 cut(s) 257, 287, 1762, 2381
TscAI CASTG 4 cut(s) 862, 1199, 1555, 2246
TseFI GTSAC 5 cut(s) 362, 1212, 1322, 1772, 2095
TseI GCWGC 5 cut(s) 1052, 1621, 2221, 2513, 2690
Tsp45I GTSAC 5 cut(s) 362, 1212, 1322, 1772, 2095
TspGWI ACGGA 3 cut(s) 1325, 1846, 2131
TspRI CASTG 4 cut(s) 862, 1199, 1555, 2246
Vha464I CTTAAG 1 cut(s) 200
VpaK11BI GGWCC 2 cut(s) 566, 599
VspI ATTAAT 1 cut(s) 482
XapI RAATTY 5 cut(s) 86, 713, 1036, 1960, 2089
XbaI TCTAGA 1 cut(s) 796
XceI RCATGY 4 cut(s) 67, 976, 1064, 1386
XhoI CTCGAG 1 cut(s) 384
XmnI GAANNNNTTC 1 cut(s) 1188
XspI CTAG 7 cut(s) 491, 524, 632, 777, 797, 1320, 2330
ZrmI AGTACT 1 cut(s) 1432
Zsp2I ATGCAT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.