Belongs to the peptidase M16 family
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Peptidase_M16_M | PF16187 | 1 - 196 | 8.9e-48 | Middle or third domain of peptidase_M16 |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 933 |
| AarI | CACCTGC | 1 cut(s) | 367 |
| Acc36I | ACCTGC | 1 cut(s) | 367 |
| AclWI | GGATC | 2 cut(s) | 11, 24 |
| AcsI | RAATTY | 1 cut(s) | 757 |
| AcuI | CTGAAG | 1 cut(s) | 179 |
| AcvI | CACGTG | 1 cut(s) | 981 |
| AfaI | GTAC | 4 cut(s) | 184, 212, 677, 693 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 336, 376 |
| AflIII | ACRYGT | 1 cut(s) | 980 |
| AgsI | TTSAA | 9 cut(s) | 117, 391, 598, 622, 766, 800, 961, 1174, 1217 |
| AjnI | CCWGG | 1 cut(s) | 648 |
| AluBI | AGCT | 9 cut(s) | 175, 298, 373, 400, 407, 441, 583, 640, 938 |
| AluI | AGCT | 9 cut(s) | 175, 298, 373, 400, 407, 441, 583, 640, 938 |
| Alw21I | GWGCWC | 1 cut(s) | 300 |
| AlwI | GGATC | 2 cut(s) | 11, 24 |
| AoxI | GGCC | 1 cut(s) | 709 |
| ApeKI | GCWGC | 3 cut(s) | 373, 1049, 1226 |
| ApoI | RAATTY | 1 cut(s) | 757 |
| AspS9I | GGNCC | 1 cut(s) | 709 |
| BamHI | GGATCC | 1 cut(s) | 16 |
| BanII | GRGCYC | 1 cut(s) | 300 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 152, 184, 774, 806 |
| BauI | CACGAG | 1 cut(s) | 1146 |
| BbrPI | CACGTG | 1 cut(s) | 981 |
| BbsI | GAAGAC | 4 cut(s) | 486, 821, 1141, 1224 |
| Bbv12I | GWGCWC | 1 cut(s) | 300 |
| BbvI | GCAGC | 3 cut(s) | 360, 1061, 1238 |
| BccI | CCATC | 2 cut(s) | 89, 92 |
| BciT130I | CCWGG | 1 cut(s) | 650 |
| BfaI | CTAG | 1 cut(s) | 72 |
| BfmI | CTRYAG | 1 cut(s) | 374 |
| BfuAI | ACCTGC | 1 cut(s) | 367 |
| BglII | AGATCT | 2 cut(s) | 162, 696 |
| BisI | GCNGC | 3 cut(s) | 374, 1050, 1227 |
| BlsI | GCNGC | 3 cut(s) | 375, 1051, 1228 |
| BmcAI | AGTACT | 1 cut(s) | 184 |
| Bme1390I | CCNGG | 1 cut(s) | 650 |
| BmgT120I | GGNCC | 1 cut(s) | 709 |
| BmiI | GGNNCC | 1 cut(s) | 18 |
| BmrFI | CCNGG | 1 cut(s) | 650 |
| BmsI | GCATC | 4 cut(s) | 52, 847, 1055, 1267 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1198 |
| BpiI | GAAGAC | 4 cut(s) | 486, 821, 1141, 1224 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 629, 661 |
| BpmI | CTGGAG | 3 cut(s) | 6, 353, 1217 |
| Bpu10I | CCTNAGC | 1 cut(s) | 636 |
| BpuEI | CTTGAG | 1 cut(s) | 559 |
| Bsa29I | ATCGAT | 1 cut(s) | 994 |
| BsaAI | YACGTR | 1 cut(s) | 981 |
| BsaJI | CCNNGG | 3 cut(s) | 518, 648, 718 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 336, 376 |
| Bse1I | ACTGG | 2 cut(s) | 912, 1200 |
| Bse3DI | GCAATG | 1 cut(s) | 152 |
| BseBI | CCWGG | 1 cut(s) | 650 |
| BseCI | ATCGAT | 1 cut(s) | 994 |
| BseDI | CCNNGG | 3 cut(s) | 518, 648, 718 |
| BseGI | GGATG | 6 cut(s) | 84, 105, 916, 971, 1087, 1282 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 336, 376 |
| BseMI | GCAATG | 1 cut(s) | 152 |
| BseMII | CTCAG | 4 cut(s) | 38, 313, 627, 1075 |
| BseNI | ACTGG | 2 cut(s) | 912, 1200 |
| BseRI | GAGGAG | 2 cut(s) | 672, 1027 |
| BseXI | GCAGC | 3 cut(s) | 360, 1061, 1238 |
| BshFI | GGCC | 1 cut(s) | 711 |
| BshVI | ATCGAT | 1 cut(s) | 994 |
| BsiHKAI | GWGCWC | 1 cut(s) | 300 |
| BsiSI | CCGG | 1 cut(s) | 131 |
| BslFI | GGGAC | 1 cut(s) | 352 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 336, 376 |
| BsmFI | GGGAC | 1 cut(s) | 352 |
| BsmI | GAATGC | 2 cut(s) | 508, 1068 |
| BsnI | GGCC | 1 cut(s) | 711 |
| Bsp1286I | GDGCHC | 1 cut(s) | 300 |
| Bsp1407I | TGTACA | 1 cut(s) | 691 |
| Bsp143I | GATC | 5 cut(s) | 16, 162, 306, 457, 696 |
| Bsp19I | CCATGG | 1 cut(s) | 718 |
| BspANI | GGCC | 1 cut(s) | 711 |
| BspCNI | CTCAG | 4 cut(s) | 39, 312, 628, 1074 |
| BspDI | ATCGAT | 1 cut(s) | 994 |
| BspLI | GGNNCC | 1 cut(s) | 18 |
| BspMAI | CTGCAG | 1 cut(s) | 378 |
| BspMI | ACCTGC | 1 cut(s) | 367 |
| BspPI | GGATC | 2 cut(s) | 11, 24 |
| BsrDI | GCAATG | 1 cut(s) | 152 |
| BsrGI | TGTACA | 1 cut(s) | 691 |
| BsrI | ACTGG | 2 cut(s) | 912, 1200 |
| BssECI | CCNNGG | 3 cut(s) | 518, 648, 718 |
| BssMI | GATC | 5 cut(s) | 16, 162, 306, 457, 696 |
| BssSI | CACGAG | 1 cut(s) | 1146 |
| BssT1I | CCWWGG | 2 cut(s) | 518, 718 |
| Bst2BI | CACGAG | 1 cut(s) | 1146 |
| Bst2UI | CCWGG | 1 cut(s) | 650 |
| Bst4CI | ACNGT | 2 cut(s) | 433, 865 |
| BstAUI | TGTACA | 1 cut(s) | 691 |
| BstBAI | YACGTR | 1 cut(s) | 981 |
| BstC8I | GCNNGC | 1 cut(s) | 331 |
| BstDEI | CTNAG | 5 cut(s) | 47, 299, 636, 1061, 1260 |
| BstDSI | CCRYGG | 1 cut(s) | 718 |
| BstF5I | GGATG | 6 cut(s) | 84, 105, 916, 971, 1087, 1282 |
| BstKTI | GATC | 5 cut(s) | 19, 165, 309, 460, 699 |
| BstMBI | GATC | 5 cut(s) | 16, 162, 306, 457, 696 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 326, 1187 |
| BstNI | CCWGG | 1 cut(s) | 650 |
| BstNSI | RCATGY | 1 cut(s) | 138 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1198 |
| BstSCI | CCNGG | 1 cut(s) | 648 |
| BstSFI | CTRYAG | 1 cut(s) | 374 |
| BstV1I | GCAGC | 3 cut(s) | 360, 1061, 1238 |
| BstV2I | GAAGAC | 4 cut(s) | 486, 821, 1141, 1224 |
| BstX2I | RGATCY | 3 cut(s) | 16, 162, 696 |
| BstYI | RGATCY | 3 cut(s) | 16, 162, 696 |
| Bsu15I | ATCGAT | 1 cut(s) | 994 |
| BsuRI | GGCC | 1 cut(s) | 711 |
| BsuTUI | ATCGAT | 1 cut(s) | 994 |
| BtgI | CCRYGG | 1 cut(s) | 718 |
| BtsCI | GGATG | 6 cut(s) | 84, 105, 916, 971, 1087, 1282 |
| BtsIMutI | CAGTG | 1 cut(s) | 307 |
| BveI | ACCTGC | 1 cut(s) | 367 |
| Cac8I | GCNNGC | 1 cut(s) | 331 |
| Cfr13I | GGNCC | 1 cut(s) | 709 |
| ClaI | ATCGAT | 1 cut(s) | 994 |
| Csp6I | GTAC | 4 cut(s) | 183, 211, 676, 692 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 405, 440 |
| CviAII | CATG | 4 cut(s) | 135, 484, 590, 719 |
| CviQI | GTAC | 4 cut(s) | 183, 211, 676, 692 |
| DdeI | CTNAG | 5 cut(s) | 47, 299, 636, 1061, 1260 |
| DpnI | GATC | 5 cut(s) | 18, 164, 308, 459, 698 |
| DpnII | GATC | 5 cut(s) | 16, 162, 306, 457, 696 |
| DraI | TTTAAA | 1 cut(s) | 190 |
| Ecl136II | GAGCTC | 1 cut(s) | 298 |
| Eco130I | CCWWGG | 2 cut(s) | 518, 718 |
| Eco24I | GRGCYC | 1 cut(s) | 300 |
| Eco53kI | GAGCTC | 1 cut(s) | 298 |
| Eco57I | CTGAAG | 1 cut(s) | 179 |
| Eco72I | CACGTG | 1 cut(s) | 981 |
| EcoICRI | GAGCTC | 1 cut(s) | 298 |
| EcoO109I | RGGNCCY | 1 cut(s) | 709 |
| EcoRII | CCWGG | 1 cut(s) | 648 |
| EcoT14I | CCWWGG | 2 cut(s) | 518, 718 |
| EcoT22I | ATGCAT | 1 cut(s) | 1070 |
| EcoT38I | GRGCYC | 1 cut(s) | 300 |
| ErhI | CCWWGG | 2 cut(s) | 518, 718 |
| FaeI | CATG | 4 cut(s) | 138, 487, 593, 722 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 428, 460, 758, 790 |
| FaqI | GGGAC | 1 cut(s) | 352 |
| FatI | CATG | 4 cut(s) | 134, 483, 589, 718 |
| FauNDI | CATATG | 1 cut(s) | 655 |
| Fnu4HI | GCNGC | 3 cut(s) | 374, 1050, 1227 |
| FokI | GGATG | 5 cut(s) | 71, 112, 923, 958, 1094 |
| FriOI | GRGCYC | 1 cut(s) | 300 |
| Fsp4HI | GCNGC | 3 cut(s) | 374, 1050, 1227 |
| FspBI | CTAG | 1 cut(s) | 72 |
| GluI | GCNGC | 3 cut(s) | 374, 1050, 1227 |
| GsuI | CTGGAG | 3 cut(s) | 6, 353, 1217 |
| HaeIII | GGCC | 1 cut(s) | 711 |
| HapII | CCGG | 1 cut(s) | 131 |
| Hin1II | CATG | 4 cut(s) | 138, 487, 593, 722 |
| HindIII | AAGCTT | 2 cut(s) | 173, 398 |
| HinfI | GANTC | 4 cut(s) | 514, 917, 1034, 1199 |
| HpaII | CCGG | 1 cut(s) | 131 |
| Hpy166II | GTNNAC | 1 cut(s) | 357 |
| Hpy188I | TCNGA | 7 cut(s) | 48, 224, 616, 733, 880, 1064, 1074 |
| Hpy188III | TCNNGA | 7 cut(s) | 23, 92, 107, 635, 766, 925, 1274 |
| Hpy8I | GTNNAC | 1 cut(s) | 357 |
| HpyAV | CCTTC | 6 cut(s) | 7, 293, 843, 896, 931, 1069 |
| HpyCH4III | ACNGT | 2 cut(s) | 433, 865 |
| HpyCH4IV | ACGT | 3 cut(s) | 573, 980, 1221 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 326, 1187 |
| HpyF3I | CTNAG | 5 cut(s) | 47, 299, 636, 1061, 1260 |
| HpySE526I | ACGT | 3 cut(s) | 573, 980, 1221 |
| Hsp92II | CATG | 4 cut(s) | 138, 487, 593, 722 |
| Kzo9I | GATC | 5 cut(s) | 16, 162, 306, 457, 696 |
| Lsp1109I | GCAGC | 3 cut(s) | 360, 1061, 1238 |
| LweI | GCATC | 4 cut(s) | 52, 847, 1055, 1267 |
| MaeI | CTAG | 1 cut(s) | 72 |
| MaeII | ACGT | 3 cut(s) | 573, 980, 1221 |
| MaeIII | GTNAC | 5 cut(s) | 74, 167, 524, 667, 892 |
| MalI | GATC | 5 cut(s) | 18, 164, 308, 459, 698 |
| MboI | GATC | 5 cut(s) | 16, 162, 306, 457, 696 |
| MflI | RGATCY | 3 cut(s) | 16, 162, 696 |
| MhlI | GDGCHC | 1 cut(s) | 300 |
| MluCI | AATT | 8 cut(s) | 5, 27, 420, 724, 744, 757, 800, 928 |
| MlyI | GAGTC | 2 cut(s) | 1028, 1208 |
| Mph1103I | ATGCAT | 1 cut(s) | 1070 |
| MseI | TTAA | 5 cut(s) | 189, 288, 474, 1017, 1233 |
| MslI | CAYNNNNRTG | 1 cut(s) | 224 |
| MspA1I | CMGCKG | 1 cut(s) | 373 |
| MspI | CCGG | 1 cut(s) | 131 |
| MspR9I | CCNGG | 1 cut(s) | 650 |
| Mva1269I | GAATGC | 2 cut(s) | 508, 1068 |
| MvaI | CCWGG | 1 cut(s) | 650 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 326, 1187 |
| NcoI | CCATGG | 1 cut(s) | 718 |
| NdeI | CATATG | 1 cut(s) | 655 |
| NdeII | GATC | 5 cut(s) | 16, 162, 306, 457, 696 |
| NlaIII | CATG | 4 cut(s) | 138, 487, 593, 722 |
| NlaIV | GGNNCC | 1 cut(s) | 18 |
| NmuCI | GTSAC | 3 cut(s) | 74, 524, 892 |
| NsiI | ATGCAT | 1 cut(s) | 1070 |
| NspI | RCATGY | 1 cut(s) | 138 |
| PaqCI | CACCTGC | 1 cut(s) | 367 |
| PctI | GAATGC | 2 cut(s) | 508, 1068 |
| PfeI | GAWTC | 2 cut(s) | 514, 917 |
| PkrI | GCNGC | 3 cut(s) | 375, 1051, 1228 |
| PleI | GAGTC | 2 cut(s) | 1028, 1207 |
| PmaCI | CACGTG | 1 cut(s) | 981 |
| PmlI | CACGTG | 1 cut(s) | 981 |
| PpsI | GAGTC | 2 cut(s) | 1028, 1207 |
| Ppu21I | YACGTR | 1 cut(s) | 981 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1198 |
| PsiI | TTATAA | 1 cut(s) | 933 |
| Psp124BI | GAGCTC | 1 cut(s) | 300 |
| Psp6I | CCWGG | 1 cut(s) | 648 |
| PspCI | CACGTG | 1 cut(s) | 981 |
| PspGI | CCWGG | 1 cut(s) | 648 |
| PspN4I | GGNNCC | 1 cut(s) | 18 |
| PspPI | GGNCC | 1 cut(s) | 709 |
| PstI | CTGCAG | 1 cut(s) | 378 |
| PsuI | RGATCY | 3 cut(s) | 16, 162, 696 |
| PvuII | CAGCTG | 1 cut(s) | 373 |
| RsaI | GTAC | 4 cut(s) | 184, 212, 677, 693 |
| RsaNI | GTAC | 4 cut(s) | 183, 211, 676, 692 |
| RseI | CAYNNNNRTG | 1 cut(s) | 224 |
| SacI | GAGCTC | 1 cut(s) | 300 |
| SaqAI | TTAA | 5 cut(s) | 189, 288, 474, 1017, 1233 |
| SatI | GCNGC | 3 cut(s) | 374, 1050, 1227 |
| Sau3AI | GATC | 5 cut(s) | 16, 162, 306, 457, 696 |
| Sau96I | GGNCC | 1 cut(s) | 709 |
| ScaI | AGTACT | 1 cut(s) | 184 |
| SchI | GAGTC | 2 cut(s) | 1028, 1208 |
| ScrFI | CCNGG | 1 cut(s) | 650 |
| SduI | GDGCHC | 1 cut(s) | 300 |
| SfaNI | GCATC | 4 cut(s) | 52, 847, 1055, 1267 |
| SfcI | CTRYAG | 1 cut(s) | 374 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 224 |
| SmlI | CTYRAG | 1 cut(s) | 538 |
| SmoI | CTYRAG | 1 cut(s) | 538 |
| Sse9I | AATT | 8 cut(s) | 5, 27, 420, 724, 744, 757, 800, 928 |
| SspMI | CTAG | 1 cut(s) | 72 |
| SstI | GAGCTC | 1 cut(s) | 300 |
| StyD4I | CCNGG | 1 cut(s) | 648 |
| StyI | CCWWGG | 2 cut(s) | 518, 718 |
| TaaI | ACNGT | 2 cut(s) | 433, 865 |
| TaiI | ACGT | 3 cut(s) | 576, 983, 1224 |
| TaqI | TCGA | 5 cut(s) | 279, 924, 994, 1132, 1244 |
| TasI | AATT | 8 cut(s) | 5, 27, 420, 724, 744, 757, 800, 928 |
| TatI | WGTACW | 3 cut(s) | 182, 675, 691 |
| TfiI | GAWTC | 2 cut(s) | 514, 917 |
| Tru1I | TTAA | 5 cut(s) | 189, 288, 474, 1017, 1233 |
| Tru9I | TTAA | 5 cut(s) | 189, 288, 474, 1017, 1233 |
| TscAI | CASTG | 1 cut(s) | 307 |
| TseFI | GTSAC | 3 cut(s) | 74, 524, 892 |
| TseI | GCWGC | 3 cut(s) | 373, 1049, 1226 |
| Tsp45I | GTSAC | 3 cut(s) | 74, 524, 892 |
| TspDTI | ATGAA | 5 cut(s) | 55, 208, 242, 527, 578 |
| TspGWI | ACGGA | 2 cut(s) | 77, 769 |
| TspRI | CASTG | 1 cut(s) | 307 |
| XapI | RAATTY | 1 cut(s) | 757 |
| XceI | RCATGY | 1 cut(s) | 138 |
| XspI | CTAG | 1 cut(s) | 72 |
| ZrmI | AGTACT | 1 cut(s) | 184 |
| Zsp2I | ATGCAT | 1 cut(s) | 1070 |