Rw5G044360

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000183
Physical Location & Seq
Reverse (-)
78053770 .. 78062092
8323 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G044360.1

Sequence Viewer

Length: 3000 bp
ATGGGTCGCCCCACTTTCTCCTCCGACGAAGTTGTGATCAAGTCTCCGAATGATAAACGACTCTACAGATTGATTGAGCTTGAGAATGGCCTCACTGCATTGCTCATTCATGATCCTGATATATACCCACAAGGGTTTAAGGGCCTTGAGCCCTGTGAAGATGAAGCAGCAGCCAAAGGTGGAGACGGTGAGGTTGGAAATAAGGGAAAGGGAGGTGCTTTGCGGACTAAGAAGGCAGCAGCAGCAATGTGCGTTGGAATAGGCAGCTGGTCTGACCCTATGGAGGCACAGGGGCTTGCACACTTTCTAGAACACATGCTGTTCATGGGGAGTACAAAATTCCCAGTTGAAAATGAGTATGATAGTTACTTGTCCAAGCATGGAGGGTCATCAAATGCATACACCTGTGCAGAGCATACTTGCTACTTTTTTGAAGTGAAACCGGAGTTTCTTAAGGGTGCCTTGACAAGATTTTCTCAGTTCTTTGTTTCACCTCTGATAAAAAGTGAATCCATGGAGCGAGAGATACAGGCTATAGATTCAGAGTTTAACAGGGTTCTGCAAAATGACTTTTGCCGCCTTCGACAACTTCAATGCTATACATCCACACCAGGTCATCCGTTTAATAGATTTTCTCGAGGAAATAAGAAGAGCTTGGATGATGCAACGAAAAAGGGGATCAACTTGCAGGAACAAATACTGAAATTGTACAGAAACTATTACCGCGGTGGATTAATGAAGCTAGTTGTCATTGGTGGAGAGTCGCTCGATGTACTAGAGAAGTGGATTTTGGAATTGTATGGAGATGTCAAAACTGGTCCCCAAGTAAATCTGGAGTTCAAGGCAGACGGTCCTATTTGGAAAGCTGGAAAAGTTTACAGGCTAGAGGCTGTTCAAGATGTTCATATACTGCAATTGTCATGGAAACTTCCATGCCTTCTTCAACACTATTTGAAAGGCCCAGAATTTTATTTACATAATCTCCTTGTGCATGAGGGCAAGGGAAGTTTGCATTTCTACCTCAAAGCTAGAGGGTGGGCAACGTATCTAGGTGCTTCGGTGAGCCGCTATCCTGTGGCCGATGTCTTTTGCACGATCATATACCTCACTGACTCTGGACTGGAGAAGATTTTTGAAATAATTGGGTTGGTGTATCAATACATTAAGTTACTACGTCAGGAGACGCCAGAATGGATATTTCGAGAACTCCAGGATATAGGGAACATGTGCTTTAGATTTGCAGAGGAGGAGCCTTTCCAGGATGATTATGTTTCAAACCTTGCAGAAAATTTACGACGTTATGCAGCAGAGCATGTTATTTATGGGGACTATGTGTTTGGGATATGGGATGAGGTATTGATACAAAATGTTCTGGGTTTCTTCTCTCCAGAAAACATGAGGATTGATGTGGTATCAAAGTTCTCATTTAAGTCAGAAGAGTTTCAGTGCGAGCCTTGGTTCGGGTCACATTATACCGAGGAAGATATATCTCCAGCTTTGATGGAATTGTGGAAGGATCCTCCAGAAATTGATGCATCGTTGCATCTGCGGGCAAAAAATGAGTTCATTCCTAGTGACTTTTCCATCCGTTCTGATGGGATGTGTCTTGATATTTCAAATATATCTTCTCCGGCATGTTTGTTTGACGAACCATTGATGAAGATCTGGTACAAGCTTGATAGTACTTTTAAACTTCCACGGGCAAATGCGTACTTCTTCATCAGAATGAAAGGTGGATATGATAATGTAAAAAGTAGTGTTTTGACAGACTTATACATCGACCTTCTTGAAGATGAGCTCAGTGAGATCATCTATCAGGCTTATGTTGCTTGCTTGGGGACTTCTATTTCTGTGTTTACCGACAAACTTCAGCTGCAGGTGTATGGTTTCAATGATAAGCTTCCAGGTCTGTTGTCAAAAATTGTGGAAACAGTAAAGAGCTTCTTGCCAACTGATGATCGTTTTGAGGTTATTAAAGAAGACATGGTGCGAGTGTATAGGAATGCCAATATGAATCCTTGGGGTCACTCAACATACTTGAGAGAGCAAGTTTTGTTGCAGAGTTTCTACAACGTAGATGAGCTGTTTCATGTTTTGAATGGGTTGTCCGTTTCTGATTTGAAGTCATTTATTCCTGAGCTTTTCTCCCAGATCTACATTGAGGGCCTTTTCCATGGTAATTTGTCAGAAGAAGAAGCAATTAGCCTCTCAAATTTATTCAAGACAATCTTTTCCGTAAAACCACTTCCTGTTGAATTGATGTATAGAAATCATTGTATTTGTCTTCCTTCTAATGCTAACCTCATTAGAGATGCTACTGTGAAGAACAAGTCAGAAACAAATTCTGTGACAGAGCTGTATTTTCAAATTGAGTGGGCAGTAGGGAGCGAGTCCGTGAGATGGAAAATTTTGATAGATCTTTTCGATGCAATTGTGAAGGAACCACTTTTTAATCAGCTAAGGACAAAGGAGCAGCTCGGGTATGGAGTTCAGTGTGGTCGGGATTTAAAATTGGGTGTTTTTGGCATTCTTTTCTCTGTTCAGTCATCTGAGTACAACCCAATCCACCTACAAGGAAGACTAGACAACTTCATAAATGGTCTGGAAGATTTGTTGGAAGGACTGGATGATGATTCTTTTGAGAATTATAAAGCTGGATTAATGGAAAATCTTTTGAAGAAAGATACATCCCTCACGTGTGAAACCAATCGATTGTGGAATGAGATTTATAATAAAAGGTATATGTTTGACTACTCAAAAAAGGCAGCAGAAGAACTCAGATGCATTCAGAAGGGGGATGCTATCAACTTTTACAAGACATATTTGCAACAATCATCTCCCAAGTGTCGAAGACTTGCAACTCGTGTTTGGGGTTGCAACACCGACTTCAAAGAGGCAGAAGTAGCACGACTGGAGTCTGTGCAAGTCATTGAAGACGTTGCAGCCTTTAAGATGTCATCGAAGTTCTATAAACCTAAGATAAATACTCTGGATACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

999

Amino Acids

114.6

Weight (kDa)

5.32

Isoelectric Point (pI)

40.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 76 - 189 1.1e-30 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 231 - 404 1.1e-16 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 411 - 696 1.2e-81 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 702 - 887 4.7e-17 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 808 - 906 1.3e-22 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2649, 2730
AarI CACCTGC 1 cut(s) 1867
Acc36I ACCTGC 1 cut(s) 1867
AccB1I GGYRCC 1 cut(s) 458
AccII CGCG 1 cut(s) 726
AciI CCGC 6 cut(s) 223, 577, 724, 726, 1066, 1549
AclWI GGATC 4 cut(s) 107, 686, 1511, 1524
AcoI YGGCCR 1 cut(s) 1077
AcsI RAATTY 6 cut(s) 338, 965, 1288, 2212, 2341, 2406
AcuI CTGAAG 1 cut(s) 1853
AcvI CACGTG 1 cut(s) 2697
AcyI GRCGYC 1 cut(s) 1184
AfaI GTAC 7 cut(s) 334, 710, 774, 1670, 1684, 1712, 2555
AfiI CCNNNNNNNGG 3 cut(s) 283, 1460, 2400
AflII CTTAAG 1 cut(s) 452
AflIII ACRYGT 2 cut(s) 1224, 2696
AjnI CCWGG 4 cut(s) 610, 1209, 1257, 1903
AjuI GAANNNNNNNTTGG 2 cut(s) 773, 805
Alw21I GWGCWC 1 cut(s) 1800
Alw26I GTCTC 3 cut(s) 48, 177, 1175
AlwI GGATC 4 cut(s) 107, 686, 1511, 1524
Ama87I CYCGRG 2 cut(s) 636, 2477
AoxI GGCC 5 cut(s) 88, 142, 958, 1077, 2164
ApoI RAATTY 6 cut(s) 338, 965, 1288, 2212, 2341, 2406
ArsI GACNNNNNNTTYG 2 cut(s) 1319, 1351
AseI ATTAAT 2 cut(s) 734, 2660
Asp700I GAANNNNTTC 1 cut(s) 1440
AspS9I GGNCC 5 cut(s) 142, 818, 851, 959, 2164
AsuHPI GGTGA 3 cut(s) 200, 483, 1072
AvaI CYCGRG 2 cut(s) 636, 2477
AvaII GGWCC 2 cut(s) 818, 851
BamHI GGATCC 1 cut(s) 1516
BanI GGYRCC 1 cut(s) 458
BanII GRGCYC 2 cut(s) 153, 1800
BarI GAAGNNNNNNTAC 4 cut(s) 1652, 1684, 2229, 2261
BauI CACGAG 1 cut(s) 2862
BbrPI CACGTG 1 cut(s) 2697
BbsI GAAGAC 5 cut(s) 1986, 2276, 2584, 2857, 2940
Bbv12I GWGCWC 1 cut(s) 1800
BccI CCATC 4 cut(s) 1495, 1589, 1592, 2394
BciT130I CCWGG 4 cut(s) 612, 1211, 1259, 1905
BciVI GTATCC 1 cut(s) 2986
BclI TGATCA 1 cut(s) 36
BcoDI GTCTC 3 cut(s) 48, 177, 1175
BfaI CTAG 8 cut(s) 308, 743, 776, 884, 1029, 1049, 1572, 2582
BfmI CTRYAG 3 cut(s) 64, 534, 1874
BfrI CTTAAG 1 cut(s) 452
BfuAI ACCTGC 1 cut(s) 1867
BfuI GTATCC 1 cut(s) 2986
BglII AGATCT 3 cut(s) 1662, 2151, 2416
BmcAI AGTACT 1 cut(s) 1684
Bme1390I CCNGG 4 cut(s) 612, 1211, 1259, 1905
Bme18I GGWCC 2 cut(s) 818, 851
BmeT110I CYCGRG 2 cut(s) 636, 2477
BmgT120I GGNCC 5 cut(s) 142, 818, 851, 959, 2164
BmiI GGNNCC 5 cut(s) 460, 820, 1251, 1518, 2442
BmrFI CCNGG 4 cut(s) 612, 1211, 1259, 1905
BmrI ACTGGG 1 cut(s) 338
BmsI GCATC 8 cut(s) 652, 1522, 1544, 1552, 2302, 2416, 2771, 2788
BmuI ACTGGG 1 cut(s) 338
BoxI GACNNNNGTC 1 cut(s) 2914
BpiI GAAGAC 5 cut(s) 1986, 2276, 2584, 2857, 2940
BplI GAGNNNNNCTC 4 cut(s) 750, 782, 2129, 2161
BpmI CTGGAG 7 cut(s) 854, 1142, 1193, 1371, 1476, 1506, 2933
Bpu10I CCTNAGC 2 cut(s) 2136, 2459
BpuEI CTTGAG 3 cut(s) 101, 167, 2059
Bsa29I ATCGAT 1 cut(s) 2710
BsaAI YACGTR 1 cut(s) 2697
BsaBI GATNNNNATC 1 cut(s) 1661
BsaHI GRCGYC 1 cut(s) 1184
BsaJI CCNNGG 7 cut(s) 513, 724, 1454, 1476, 1697, 2018, 2173
BsaWI WCCGGW 1 cut(s) 442
BsaXI ACNNNNNCTCC 2 cut(s) 966, 996
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 1460, 2400
Bse1I ACTGG 5 cut(s) 344, 820, 1125, 2628, 2916
Bse3DI GCAATG 2 cut(s) 98, 252
Bse8I GATNNNNATC 1 cut(s) 1661
BseBI CCWGG 4 cut(s) 612, 1211, 1259, 1905
BseCI ATCGAT 1 cut(s) 2710
BseDI CCNNGG 7 cut(s) 513, 724, 1454, 1476, 1697, 2018, 2173
BseJI GATNNNNATC 1 cut(s) 1661
BseLI CCNNNNNNNGG 3 cut(s) 283, 1460, 2400
BseMI GCAATG 2 cut(s) 98, 252
BseMII CTCAG 5 cut(s) 491, 1813, 2127, 2541, 2791
BseNI ACTGG 5 cut(s) 344, 820, 1125, 2628, 2916
BseRI GAGGAG 3 cut(s) 10, 1259, 1262
BsgI GTGCAG 1 cut(s) 429
Bsh1236I CGCG 1 cut(s) 726
BshFI GGCC 5 cut(s) 90, 144, 960, 1079, 2166
BshNI GGYRCC 1 cut(s) 458
BshVI ATCGAT 1 cut(s) 2710
BsiHKAI GWGCWC 1 cut(s) 1800
BsiHKCI CYCGRG 2 cut(s) 636, 2477
BsiSI CCGG 2 cut(s) 443, 1631
BslFI GGGAC 3 cut(s) 804, 1340, 1852
BslI CCNNNNNNNGG 3 cut(s) 283, 1460, 2400
BsmAI GTCTC 3 cut(s) 48, 177, 1175
BsmBI CGTCTC 2 cut(s) 177, 1175
BsmFI GGGAC 3 cut(s) 804, 1340, 1852
BsmI GAATGC 3 cut(s) 2008, 2526, 2784
BsnI GGCC 5 cut(s) 90, 144, 960, 1079, 2166
BsoBI CYCGRG 2 cut(s) 636, 2477
Bsp1286I GDGCHC 2 cut(s) 153, 1800
Bsp1407I TGTACA 1 cut(s) 708
Bsp19I CCATGG 2 cut(s) 513, 2173
BspACI CCGC 6 cut(s) 223, 577, 724, 726, 1066, 1549
BspANI GGCC 5 cut(s) 90, 144, 960, 1079, 2166
BspCNI CTCAG 5 cut(s) 490, 1812, 2128, 2542, 2790
BspDI ATCGAT 1 cut(s) 2710
BspFNI CGCG 1 cut(s) 726
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 5 cut(s) 460, 820, 1251, 1518, 2442
BspMAI CTGCAG 1 cut(s) 1878
BspMI ACCTGC 1 cut(s) 1867
BspPI GGATC 4 cut(s) 107, 686, 1511, 1524
BspQI GCTCTTC 1 cut(s) 644
BspT107I GGYRCC 1 cut(s) 458
BspTI CTTAAG 1 cut(s) 452
BsrDI GCAATG 2 cut(s) 98, 252
BsrGI TGTACA 1 cut(s) 708
BsrI ACTGG 5 cut(s) 344, 820, 1125, 2628, 2916
BssECI CCNNGG 7 cut(s) 513, 724, 1454, 1476, 1697, 2018, 2173
BssNI GRCGYC 1 cut(s) 1184
BssSI CACGAG 1 cut(s) 2862
BssT1I CCWWGG 4 cut(s) 513, 1454, 2018, 2173
Bst2BI CACGAG 1 cut(s) 2862
Bst2UI CCWGG 4 cut(s) 612, 1211, 1259, 1905
Bst4CI ACNGT 4 cut(s) 188, 851, 1933, 2320
Bst6I CTCTTC 2 cut(s) 644, 1431
BstACI GRCGYC 1 cut(s) 1184
BstAFI CTTAAG 1 cut(s) 452
BstAUI TGTACA 1 cut(s) 708
BstBAI YACGTR 1 cut(s) 2697
BstC8I GCNNGC 4 cut(s) 297, 1451, 1551, 1831
BstDEI CTNAG 8 cut(s) 228, 477, 1799, 2136, 2459, 2550, 2777, 2976
BstDSI CCRYGG 4 cut(s) 513, 724, 1697, 2173
BstFNI CGCG 1 cut(s) 726
BstMAI GTCTC 3 cut(s) 48, 177, 1175
BstMWI GCNNNNNNNGC 3 cut(s) 242, 1826, 2903
BstNI CCWGG 4 cut(s) 612, 1211, 1259, 1905
BstNSI RCATGY 4 cut(s) 319, 1228, 1316, 1638
BstPAI GACNNNNGTC 1 cut(s) 2914
BstSCI CCNGG 4 cut(s) 610, 1209, 1257, 1903
BstSFI CTRYAG 3 cut(s) 64, 534, 1874
BstUI CGCG 1 cut(s) 726
BstV2I GAAGAC 5 cut(s) 1986, 2276, 2584, 2857, 2940
BstX2I RGATCY 4 cut(s) 1516, 1662, 2151, 2416
BstYI RGATCY 4 cut(s) 1516, 1662, 2151, 2416
Bsu15I ATCGAT 1 cut(s) 2710
BsuI GTATCC 1 cut(s) 2986
BsuRI GGCC 5 cut(s) 90, 144, 960, 1079, 2166
BsuTUI ATCGAT 1 cut(s) 2710
BtgI CCRYGG 4 cut(s) 513, 724, 1697, 2173
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 5 cut(s) 93, 1107, 1451, 1807, 2498
BveI ACCTGC 1 cut(s) 1867
Cac8I GCNNGC 4 cut(s) 297, 1451, 1551, 1831
CciI TCATGA 1 cut(s) 109
Cfr13I GGNCC 5 cut(s) 142, 818, 851, 959, 2164
Cfr42I CCGCGG 1 cut(s) 727
ClaI ATCGAT 1 cut(s) 2710
CseI GACGC 1 cut(s) 1192
CsiI ACCWGGT 1 cut(s) 610
Csp6I GTAC 7 cut(s) 333, 709, 773, 1669, 1683, 1711, 2554
CspCI CAANNNNNGTGG 4 cut(s) 1905, 1940, 2354, 2389
CviQI GTAC 7 cut(s) 333, 709, 773, 1669, 1683, 1711, 2554
DdeI CTNAG 8 cut(s) 228, 477, 1799, 2136, 2459, 2550, 2777, 2976
DraI TTTAAA 2 cut(s) 1690, 2508
EaeI YGGCCR 1 cut(s) 1077
Eam1104I CTCTTC 2 cut(s) 644, 1431
EarI CTCTTC 2 cut(s) 644, 1431
Ecl136II GAGCTC 1 cut(s) 1798
Eco130I CCWWGG 4 cut(s) 513, 1454, 2018, 2173
Eco24I GRGCYC 2 cut(s) 153, 1800
Eco47I GGWCC 2 cut(s) 818, 851
Eco53kI GAGCTC 1 cut(s) 1798
Eco57I CTGAAG 1 cut(s) 1853
Eco72I CACGTG 1 cut(s) 2697
Eco88I CYCGRG 2 cut(s) 636, 2477
EcoICRI GAGCTC 1 cut(s) 1798
EcoO109I RGGNCCY 2 cut(s) 142, 2164
EcoRII CCWGG 4 cut(s) 610, 1209, 1257, 1903
EcoT14I CCWWGG 4 cut(s) 513, 1454, 2018, 2173
EcoT22I ATGCAT 3 cut(s) 400, 1537, 2786
EcoT38I GRGCYC 2 cut(s) 153, 1800
ErhI CCWWGG 4 cut(s) 513, 1454, 2018, 2173
Esp3I CGTCTC 2 cut(s) 177, 1175
FalI AAGNNNNNCTT 8 cut(s) 446, 478, 638, 670, 1928, 1960, 2213, 2245
FaqI GGGAC 3 cut(s) 804, 1340, 1852
FauI CCCGC 1 cut(s) 1542
FbaI TGATCA 1 cut(s) 36
FriOI GRGCYC 2 cut(s) 153, 1800
FspBI CTAG 8 cut(s) 308, 743, 776, 884, 1029, 1049, 1572, 2582
GsuI CTGGAG 7 cut(s) 854, 1142, 1193, 1371, 1476, 1506, 2933
HaeIII GGCC 5 cut(s) 90, 144, 960, 1079, 2166
HapII CCGG 2 cut(s) 443, 1631
HgaI GACGC 1 cut(s) 1192
Hin1I GRCGYC 1 cut(s) 1184
HindIII AAGCTT 2 cut(s) 1673, 1898
HinfI GANTC 9 cut(s) 60, 509, 539, 761, 1112, 2014, 2390, 2633, 2915
HpaII CCGG 2 cut(s) 443, 1631
HphI GGTGA 3 cut(s) 200, 483, 1072
Hpy166II GTNNAC 2 cut(s) 877, 1857
Hpy8I GTNNAC 2 cut(s) 877, 1857
Hpy99I CGWCG 2 cut(s) 29, 1299
HpyAV CCTTC 9 cut(s) 226, 590, 947, 1507, 1793, 2298, 2431, 2612, 2785
HpyCH4III ACNGT 4 cut(s) 188, 851, 1933, 2320
HpyCH4IV ACGT 6 cut(s) 1043, 1174, 1297, 2073, 2696, 2937
HpyF10VI GCNNNNNNNGC 3 cut(s) 242, 1826, 2903
HpyF3I CTNAG 8 cut(s) 228, 477, 1799, 2136, 2459, 2550, 2777, 2976
HpySE526I ACGT 6 cut(s) 1043, 1174, 1297, 2073, 2696, 2937
Hsp92I GRCGYC 1 cut(s) 1184
Ksp22I TGATCA 1 cut(s) 36
KspI CCGCGG 1 cut(s) 727
LguI GCTCTTC 1 cut(s) 644
LmnI GCTCC 4 cut(s) 517, 1249, 2385, 2470
LweI GCATC 8 cut(s) 652, 1522, 1544, 1552, 2302, 2416, 2771, 2788
MabI ACCWGGT 1 cut(s) 610
MaeI CTAG 8 cut(s) 308, 743, 776, 884, 1029, 1049, 1572, 2582
MaeII ACGT 6 cut(s) 1043, 1174, 1297, 2073, 2696, 2937
MaeIII GTNAC 6 cut(s) 365, 1167, 1464, 1574, 2024, 2347
MfeI CAATTG 2 cut(s) 914, 2430
MflI RGATCY 4 cut(s) 1516, 1662, 2151, 2416
MhlI GDGCHC 2 cut(s) 153, 1800
MlyI GAGTC 5 cut(s) 54, 770, 1106, 2399, 2924
MmeI TCCRAC 4 cut(s) 48, 175, 235, 2595
Mph1103I ATGCAT 3 cut(s) 400, 1537, 2786
MroXI GAANNNNTTC 1 cut(s) 1440
MslI CAYNNNNRTG 1 cut(s) 1724
MspA1I CMGCKG 3 cut(s) 267, 726, 1873
MspCI CTTAAG 1 cut(s) 452
MspI CCGG 2 cut(s) 443, 1631
MspR9I CCNGG 4 cut(s) 612, 1211, 1259, 1905
MunI CAATTG 2 cut(s) 914, 2430
Mva1269I GAATGC 3 cut(s) 2008, 2526, 2784
MvaI CCWGG 4 cut(s) 612, 1211, 1259, 1905
MvnI CGCG 1 cut(s) 726
MwoI GCNNNNNNNGC 3 cut(s) 242, 1826, 2903
NcoI CCATGG 2 cut(s) 513, 2173
NlaIV GGNNCC 5 cut(s) 460, 820, 1251, 1518, 2442
NmuCI GTSAC 4 cut(s) 1464, 1574, 2024, 2347
NsiI ATGCAT 3 cut(s) 400, 1537, 2786
NspI RCATGY 4 cut(s) 319, 1228, 1316, 1638
PaeR7I CTCGAG 1 cut(s) 636
PagI TCATGA 1 cut(s) 109
PaqCI CACCTGC 1 cut(s) 1867
PciI ACATGT 1 cut(s) 1224
PciSI GCTCTTC 1 cut(s) 644
PctI GAATGC 3 cut(s) 2008, 2526, 2784
PdmI GAANNNNTTC 1 cut(s) 1440
PfeI GAWTC 4 cut(s) 509, 539, 2014, 2633
PfoI TCCNGGA 2 cut(s) 1209, 1257
PleI GAGTC 5 cut(s) 54, 769, 1106, 2398, 2923
PmaCI CACGTG 1 cut(s) 2697
PmlI CACGTG 1 cut(s) 2697
PpsI GAGTC 5 cut(s) 54, 769, 1106, 2398, 2923
Ppu21I YACGTR 1 cut(s) 2697
PscI ACATGT 1 cut(s) 1224
PshAI GACNNNNGTC 1 cut(s) 2914
PshBI ATTAAT 2 cut(s) 734, 2660
PsiI TTATAA 2 cut(s) 2649, 2730
Psp124BI GAGCTC 1 cut(s) 1800
Psp6I CCWGG 4 cut(s) 610, 1209, 1257, 1903
PspCI CACGTG 1 cut(s) 2697
PspGI CCWGG 4 cut(s) 610, 1209, 1257, 1903
PspN4I GGNNCC 5 cut(s) 460, 820, 1251, 1518, 2442
PspPI GGNCC 5 cut(s) 142, 818, 851, 959, 2164
PstI CTGCAG 1 cut(s) 1878
PsuI RGATCY 4 cut(s) 1516, 1662, 2151, 2416
PvuII CAGCTG 2 cut(s) 267, 1873
RsaI GTAC 7 cut(s) 334, 710, 774, 1670, 1684, 1712, 2555
RsaNI GTAC 7 cut(s) 333, 709, 773, 1669, 1683, 1711, 2554
RseI CAYNNNNRTG 1 cut(s) 1724
SacI GAGCTC 1 cut(s) 1800
SacII CCGCGG 1 cut(s) 727
SapI GCTCTTC 1 cut(s) 644
Sau96I GGNCC 5 cut(s) 142, 818, 851, 959, 2164
ScaI AGTACT 1 cut(s) 1684
SchI GAGTC 5 cut(s) 54, 770, 1106, 2399, 2924
ScrFI CCNGG 4 cut(s) 612, 1211, 1259, 1905
SduI GDGCHC 2 cut(s) 153, 1800
SexAI ACCWGGT 1 cut(s) 610
SfaNI GCATC 8 cut(s) 652, 1522, 1544, 1552, 2302, 2416, 2771, 2788
SfcI CTRYAG 3 cut(s) 64, 534, 1874
Sfr274I CTCGAG 1 cut(s) 636
Sfr303I CCGCGG 1 cut(s) 727
SgrBI CCGCGG 1 cut(s) 727
SinI GGWCC 2 cut(s) 818, 851
SlaI CTCGAG 1 cut(s) 636
SmiMI CAYNNNNRTG 1 cut(s) 1724
SmlI CTYRAG 5 cut(s) 80, 146, 452, 636, 2038
SmoI CTYRAG 5 cut(s) 80, 146, 452, 636, 2038
SsiI CCGC 6 cut(s) 223, 577, 724, 726, 1066, 1549
SspMI CTAG 8 cut(s) 308, 743, 776, 884, 1029, 1049, 1572, 2582
SstI GAGCTC 1 cut(s) 1800
StyD4I CCNGG 4 cut(s) 610, 1209, 1257, 1903
StyI CCWWGG 4 cut(s) 513, 1454, 2018, 2173
TaaI ACNGT 4 cut(s) 188, 851, 1933, 2320
TaiI ACGT 6 cut(s) 1046, 1177, 1300, 2076, 2699, 2940
TaqI TCGA 9 cut(s) 583, 637, 768, 1201, 1779, 2424, 2710, 2848, 2960
TatI WGTACW 5 cut(s) 332, 708, 772, 1682, 2553
TauI GCSGC 2 cut(s) 579, 1068
TfiI GAWTC 4 cut(s) 509, 539, 2014, 2633
TscAI CASTG 5 cut(s) 100, 1114, 1451, 1807, 2498
TseFI GTSAC 4 cut(s) 1464, 1574, 2024, 2347
Tsp45I GTSAC 4 cut(s) 1464, 1574, 2024, 2347
TspGWI ACGGA 5 cut(s) 609, 1577, 2098, 2224, 2383
TspRI CASTG 5 cut(s) 100, 1114, 1451, 1807, 2498
Vha464I CTTAAG 1 cut(s) 452
VpaK11BI GGWCC 2 cut(s) 818, 851
VspI ATTAAT 2 cut(s) 734, 2660
XapI RAATTY 6 cut(s) 338, 965, 1288, 2212, 2341, 2406
XbaI TCTAGA 1 cut(s) 307
XceI RCATGY 4 cut(s) 319, 1228, 1316, 1638
XhoI CTCGAG 1 cut(s) 636
XmnI GAANNNNTTC 1 cut(s) 1440
XspI CTAG 8 cut(s) 308, 743, 776, 884, 1029, 1049, 1572, 2582
ZrmI AGTACT 1 cut(s) 1684
Zsp2I ATGCAT 3 cut(s) 400, 1537, 2786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.