Rw7G017220

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000183
Physical Location & Seq
Forward (+)
17908837 .. 17920490
11654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G017220.1

Sequence Viewer

Length: 2958 bp
ATGGGTCGCTGCAATTTTTCATCGGACGACATTGTGATCAAGTCTCCTAATGATAACAAACTCTACAGATTGATTACGCTTGAGAATGGCCTCACTGCATTGATCCTTCACGATCCTGAGATTTACCCACAACCCTCCACTGAAGCTGAGATTCATCCATTGCTTCAACAAAATGGTGAACTCGAGAACAAAGAAAAGGGAGGTGCTTTTCAGACCAAGAAGGCAGCAGCAGCAATGTTTGTTGGAATGGGCAGCTTCTCGGACCCTCCTGAGGCACAGGGGCTTGCACACTTTCTAGAACACATGCTCTTCATGGGGAGTACAGCGTTTCAAGATGAAAATGAGTATGACAGTTACTTGTCTAAGCACGGAGGGTCATCAAATGCATGCACTCGTGAAGAGAATACTTCGTACCACTTTGAGGTGAAACCAGAGTTTCTTAAGGGTGCCTTGTCAAGATTTTCTCAGTTCTTTGTTTCACCTCTAATGAAAAGTGAAGCCATGGAGAGAGAGTTACAGTCTATAGATTCAGAGTTCAACCGGGTTCTGCAAAATGATTTTTGTCGCCTTGAACAACTTCAAGGCCATACAGCCACACCTGGTCACCCATTTAATAAATTCACTTGGGGAAATAAGAAAAGCTTGGATGATGCAAGGGAAAGGGGGATAAACTTGCGTCAGCAAATACTGAAATTGTACAGAGACTATTACCATGGTGGATTGATGAAGCTAGTTGTCATTGGTGGAGAATCTCTTGATATACTTGAGCAGTGGGTTTTGGAATTGTTTGGAGATATCAAAAAAGGTCCCCAAGTAAACCTGGAGTTCAAGGCAGAGAGTCCTATTTGGAATGCTGGAAAACTTTACAGGCTAGAGGCTGTTAATGATGTTCACATGCTCCACTTAGCATGGACACTTCCATGTCTTAATGAACACTATTTGAAAAGCCCAGATTATTTTTTAAGTCATCTCCTTGAGCATGAGGGCAGGGGAAGTCTGTATTTTTATCTCAAAGCTAGAGGGTGGGCAACATATCTCAGTGCTTCAGTGAACCGCAGTTCTGTGGCTGATGTCTTTTGCATGATCATATACCTTACTGACTCTGGATTGGTGCAGATTTTTGAGATAATTGGGTTGGTGTATCAATACATTAAATTATTGCGTCAAGTATCACCGCAAGAATGGATATTCCAGGAACTCCAGGATATGGGGAACATAAGCTTTAGATTTTTACAGGATCAACCACAGGATGAATATGCTGTAAAACTTGGAGGAAATTTACTACGCTATTCACCAGAACATGTTATTTTTGGGGACTATGTGTACGAGACTTGGGATGAGGAATTGATAAAATATGTTCTTGGTTTCTTCAGACCAGAAAACATGAGGATTGATGTGCTATCAAAGTCCTCATTTAATTCAGAAGATTTCCAGTGCGAGCCTTGGTTTGGGTCACATTATACTGAAGAAGATATATCTCCAACTTTGATGAAGTTGTGGAAGGATCCTCCAGAAGTTGATATTTCATTGCATCTCCCTCAAAAGAATATATTCATTCCTGGTGATTTCTCCATCCGTTCAGATGTTTGTTGTCCTGATATTGCAATTATATCTTCTCCAAGATGTATACTTGATGAACCATTGGTGAAGATCTGGTACAAGCTTGACAGTACTTTTAAACAACCACGGGTAAATGCATACTTCCGCATAAATCTGAAGGGTGGATATGATAATGTCAAGAGTTATGTTTTGACTGAACTGTATATCGAGCTTCTTGAGGATGAGCTGAATGAGATCATCTATCAGGCCAATGTTGCTGGGCTGGGAACTCATGTATATGGGTCTAACGACTTTATGGAGCTGAAGGTTTACGGTTTCAATGATAAGCTCCCAGCTTTATTGTCAAAAATTCTGACAGTAGCTAGAAATTTCTTGCCAACATATGATCGTTTCGAGGTTATTAAAGAAAGAATGGAGCGAATGTTAAAGAACACCAATATGGATCCTCTGAGTCACTCTTCATACTTGAGACGTCAAGTTTTGTACCATAGATACTATGATGTAGATGAAGAGTTGGAAGTTTTAAACAGTTTGTCTGTTTCTGATATGAAGTCGTTTATTCCTGAGCTTTGTTCCCAGCTTTACATTGAAGGCCTTTGTCATGGAAATTTGTTAGAAGAAGAGGCAATCAGCCTTTCCAAAATATTTAAAACAAAATTTTCTGTAGAACCACTTCCTATCGAATTGAGGCATAAGGATCACTGTATGTGCCTTCCTCCTTATGCTAACCTCATTAGAGAAGCCAGTGTGAACAATAATTCTGAAACAAACTCTGTATTTGAGCTATATTTTCAAATTGAGATTGTGTCCACCAGACTGAGTGCGTTGGCAAAACTTTTTCAGAAAATTGTAAAGGAACCGCTTTACAATCAGCTAAGGACGAAGGAGCAGCTTGGCTATGTTGTGTGGTGTGGCCTGCGAGAAATACGTCGTGTATATGGCTTTGTTTTCTGTGTCCAGTCATCTGAGTACAACCCAATCTACTTACAAGGAAGAGTTGACAACTTTATAAATGGTGTGGATCAGTTGTTGCATGGACTGGATGATGATTCCTTTCAGAATTATAAAGATGGACTAATGGCAAATCTATTGGAGAGAGATGAAACCCTCGCACGTGAAACATCTAGGCTTTGGGAGGAGATTACCAATAAAAGGTATATGTATGACTGGCCAGAAAAGGTAGTTGAAGAGGTCAGAAGCCTTCAGAAGGAGGACGTTATTAACTTTTACAAAACGTATTTGCAACCATCATCTCCCAAGTGTCGGAGACTTGCAATTCGTGTTTGGGGTTGCAACACTGATTTCAAAGAAGCTGAAGCACCACCGGAGTCTATACAAGTCATTCAGGACCTTGCAGCCTTTAAGATGTCATCTGAGTTCTATCCTCATGGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

985

Amino Acids

114.16

Weight (kDa)

5.22

Isoelectric Point (pI)

45.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 71 - 199 3.9e-31 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 226 - 401 3.4e-20 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 408 - 693 2.4e-84 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 698 - 879 1.6e-11 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 799 - 898 1.8e-27 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2570, 2625
AatII GACGTC 1 cut(s) 2035
AccB1I GGYRCC 1 cut(s) 446
AccB7I CCANNNNNTGG 1 cut(s) 1207
AccI GTMKAC 1 cut(s) 1627
AciI CCGC 4 cut(s) 1054, 1175, 1705, 2420
AclWI GGATC 9 cut(s) 97, 107, 1245, 1499, 1512, 1997, 2010, 2265, 2589
AcoI YGGCCR 1 cut(s) 2729
AcsI RAATTY 6 cut(s) 617, 1276, 1908, 1927, 2167, 2216
AcuI CTGAAG 8 cut(s) 162, 1029, 1354, 1485, 1736, 1883, 2747, 2895
AcvI CACGTG 1 cut(s) 2675
AcyI GRCGYC 1 cut(s) 2032
AfaI GTAC 8 cut(s) 322, 413, 698, 1325, 1658, 1672, 2045, 2531
AfiI CCNNNNNNNGG 7 cut(s) 271, 421, 1207, 1448, 2712, 2767, 2823
AflII CTTAAG 1 cut(s) 440
AflIII ACRYGT 1 cut(s) 1300
AjnI CCWGG 5 cut(s) 598, 819, 1191, 1200, 1558
Alw26I GTCTC 5 cut(s) 48, 696, 1322, 2023, 2821
AlwI GGATC 9 cut(s) 97, 107, 1245, 1499, 1512, 1997, 2010, 2265, 2589
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 6 cut(s) 88, 583, 1806, 2152, 2473, 2729
ApeKI GCWGC 7 cut(s) 9, 224, 227, 230, 252, 2449, 2915
ApoI RAATTY 6 cut(s) 617, 1276, 1908, 1927, 2167, 2216
ArsI GACNNNNNNTTYG 2 cut(s) 2820, 2852
Asp700I GAANNNNTTC 3 cut(s) 576, 1550, 2232
AspS9I GGNCC 3 cut(s) 262, 806, 2908
AsuC2I CCSGG 1 cut(s) 542
AsuHPI GGTGA 8 cut(s) 188, 436, 471, 596, 1164, 1284, 1574, 1657
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 3 cut(s) 262, 806, 2908
AxyI CCTNAGG 1 cut(s) 270
BalI TGGCCA 1 cut(s) 2731
BamHI GGATCC 2 cut(s) 1504, 2002
BanI GGYRCC 1 cut(s) 446
BarI GAAGNNNNNNTAC 4 cut(s) 1640, 1672, 2217, 2249
BauI CACGAG 1 cut(s) 393
BbrPI CACGTG 1 cut(s) 2675
BbvI GCAGC 6 cut(s) 236, 239, 242, 264, 2461, 2927
BccI CCATC 3 cut(s) 1580, 2624, 2815
BciT130I CCWGG 5 cut(s) 600, 821, 1193, 1202, 1560
BclI TGATCA 2 cut(s) 36, 1083
BcnI CCSGG 1 cut(s) 542
BcoDI GTCTC 5 cut(s) 48, 696, 1322, 2023, 2821
BfaI CTAG 6 cut(s) 296, 731, 872, 1017, 1923, 2685
BfmI CTRYAG 3 cut(s) 64, 522, 2223
BfrI CTTAAG 1 cut(s) 440
BglII AGATCT 1 cut(s) 1650
BisI GCNGC 7 cut(s) 10, 225, 228, 231, 253, 2450, 2916
BlsI GCNGC 7 cut(s) 11, 226, 229, 232, 254, 2451, 2917
BmcAI AGTACT 1 cut(s) 1672
Bme1390I CCNGG 6 cut(s) 542, 600, 821, 1193, 1202, 1560
Bme18I GGWCC 3 cut(s) 262, 806, 2908
BmeT110I CYCGRG 1 cut(s) 182
BmgT120I GGNCC 3 cut(s) 262, 806, 2908
BmiI GGNNCC 6 cut(s) 264, 448, 808, 1506, 2004, 2418
BmrFI CCNGG 6 cut(s) 542, 600, 821, 1193, 1202, 1560
BmsI GCATC 2 cut(s) 640, 1540
BpmI CTGGAG 3 cut(s) 842, 1184, 1494
Bpu10I CCTNAGC 2 cut(s) 2124, 2435
BpuEI CTTGAG 5 cut(s) 101, 785, 995, 1796, 2047
BpuMI CCSGG 1 cut(s) 542
BsaAI YACGTR 1 cut(s) 2675
BsaHI GRCGYC 1 cut(s) 2032
BsaJI CCNNGG 4 cut(s) 501, 712, 1442, 1685
BsaWI WCCGGW 1 cut(s) 2884
BsaXI ACNNNNNCTCC 4 cut(s) 497, 527, 1967, 1997
Bsc4I CCNNNNNNNGG 7 cut(s) 271, 421, 1207, 1448, 2712, 2767, 2823
Bse1I ACTGG 5 cut(s) 1432, 2304, 2518, 2604, 2732
Bse21I CCTNAGG 1 cut(s) 270
Bse3DI GCAATG 3 cut(s) 158, 240, 1526
BseBI CCWGG 5 cut(s) 600, 821, 1193, 1202, 1560
BseDI CCNNGG 4 cut(s) 501, 712, 1442, 1685
BseGI GGATG 7 cut(s) 154, 652, 1255, 1342, 1572, 1786, 2608
BseLI CCNNNNNNNGG 7 cut(s) 271, 421, 1207, 1448, 2712, 2767, 2823
BseMI GCAATG 3 cut(s) 158, 240, 1526
BseNI ACTGG 5 cut(s) 1432, 2304, 2518, 2604, 2732
BseRI GAGGAG 1 cut(s) 2711
BseXI GCAGC 6 cut(s) 236, 239, 242, 264, 2461, 2927
BseYI CCCAGC 4 cut(s) 1817, 1822, 1891, 2136
BsgI GTGCAG 1 cut(s) 1133
BshFI GGCC 6 cut(s) 90, 585, 1808, 2154, 2475, 2731
BshNI GGYRCC 1 cut(s) 446
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 2 cut(s) 541, 2885
BslFI GGGAC 2 cut(s) 792, 1328
BslI CCNNNNNNNGG 7 cut(s) 271, 421, 1207, 1448, 2712, 2767, 2823
BsmAI GTCTC 5 cut(s) 48, 696, 1322, 2023, 2821
BsmBI CGTCTC 1 cut(s) 2023
BsmFI GGGAC 2 cut(s) 792, 1328
BsmI GAATGC 1 cut(s) 856
BsnI GGCC 6 cut(s) 90, 585, 1808, 2154, 2475, 2731
BsoBI CYCGRG 1 cut(s) 182
Bsp1407I TGTACA 1 cut(s) 696
Bsp19I CCATGG 2 cut(s) 501, 712
BspACI CCGC 4 cut(s) 1054, 1175, 1705, 2420
BspANI GGCC 6 cut(s) 90, 585, 1808, 2154, 2475, 2731
BspLI GGNNCC 6 cut(s) 264, 448, 808, 1506, 2004, 2418
BspPI GGATC 9 cut(s) 97, 107, 1245, 1499, 1512, 1997, 2010, 2265, 2589
BspQI GCTCTTC 1 cut(s) 314
BspT107I GGYRCC 1 cut(s) 446
BspTI CTTAAG 1 cut(s) 440
BsrDI GCAATG 3 cut(s) 158, 240, 1526
BsrGI TGTACA 1 cut(s) 696
BsrI ACTGG 5 cut(s) 1432, 2304, 2518, 2604, 2732
BssECI CCNNGG 4 cut(s) 501, 712, 1442, 1685
BssNAI GTATAC 1 cut(s) 1628
BssNI GRCGYC 1 cut(s) 2032
BssSI CACGAG 1 cut(s) 393
BssT1I CCWWGG 3 cut(s) 501, 712, 1442
Bst1107I GTATAC 1 cut(s) 1628
Bst2BI CACGAG 1 cut(s) 393
Bst2UI CCWGG 5 cut(s) 600, 821, 1193, 1202, 1560
Bst4CI ACNGT 8 cut(s) 353, 519, 1670, 1761, 1874, 1918, 2090, 2264
Bst6I CTCTTC 7 cut(s) 314, 393, 2023, 2064, 2175, 2548, 2742
BstACI GRCGYC 1 cut(s) 2032
BstAFI CTTAAG 1 cut(s) 440
BstAUI TGTACA 1 cut(s) 696
BstBAI YACGTR 1 cut(s) 2675
BstC8I GCNNGC 4 cut(s) 285, 388, 1439, 2477
BstDSI CCRYGG 3 cut(s) 501, 712, 1685
BstEII GGTNACC 1 cut(s) 602
BstENI CCTNNNNNAGG 1 cut(s) 2765
BstF5I GGATG 7 cut(s) 154, 652, 1255, 1342, 1572, 1786, 2608
BstMAI GTCTC 5 cut(s) 48, 696, 1322, 2023, 2821
BstMWI GCNNNNNNNGC 2 cut(s) 230, 1814
BstNI CCWGG 5 cut(s) 600, 821, 1193, 1202, 1560
BstNSI RCATGY 4 cut(s) 307, 390, 898, 1304
BstPI GGTNACC 1 cut(s) 602
BstSCI CCNGG 6 cut(s) 540, 598, 819, 1191, 1200, 1558
BstSFI CTRYAG 3 cut(s) 64, 522, 2223
BstV1I GCAGC 6 cut(s) 236, 239, 242, 264, 2461, 2927
BstX2I RGATCY 3 cut(s) 1504, 1650, 2002
BstYI RGATCY 3 cut(s) 1504, 1650, 2002
BstZ17I GTATAC 1 cut(s) 1628
Bsu36I CCTNAGG 1 cut(s) 270
BsuRI GGCC 6 cut(s) 90, 585, 1808, 2154, 2475, 2731
BtgI CCRYGG 3 cut(s) 501, 712, 1685
BtsCI GGATG 7 cut(s) 154, 652, 1255, 1342, 1572, 1786, 2608
BtsI GCAGTG 2 cut(s) 93, 776
BtsIMutI CAGTG 9 cut(s) 93, 138, 776, 1045, 1053, 1439, 2260, 2311, 2856
Cac8I GCNNGC 4 cut(s) 285, 388, 1439, 2477
Cfr13I GGNCC 3 cut(s) 262, 806, 2908
CseI GACGC 2 cut(s) 665, 1151
CsiI ACCWGGT 1 cut(s) 598
Csp6I GTAC 8 cut(s) 321, 412, 697, 1324, 1657, 1671, 2044, 2530
CviQI GTAC 8 cut(s) 321, 412, 697, 1324, 1657, 1671, 2044, 2530
DraI TTTAAA 3 cut(s) 1678, 2085, 2209
EaeI YGGCCR 1 cut(s) 2729
Eam1104I CTCTTC 7 cut(s) 314, 393, 2023, 2064, 2175, 2548, 2742
EarI CTCTTC 7 cut(s) 314, 393, 2023, 2064, 2175, 2548, 2742
Eco130I CCWWGG 3 cut(s) 501, 712, 1442
Eco147I AGGCCT 1 cut(s) 2154
Eco32I GATATC 1 cut(s) 796
Eco47I GGWCC 3 cut(s) 262, 806, 2908
Eco57I CTGAAG 8 cut(s) 162, 1029, 1354, 1485, 1736, 1883, 2747, 2895
Eco72I CACGTG 1 cut(s) 2675
Eco81I CCTNAGG 1 cut(s) 270
Eco88I CYCGRG 1 cut(s) 182
Eco91I GGTNACC 1 cut(s) 602
EcoNI CCTNNNNNAGG 1 cut(s) 2765
EcoO109I RGGNCCY 2 cut(s) 806, 2908
EcoO65I GGTNACC 1 cut(s) 602
EcoRII CCWGG 5 cut(s) 598, 819, 1191, 1200, 1558
EcoRV GATATC 1 cut(s) 796
EcoT14I CCWWGG 3 cut(s) 501, 712, 1442
EcoT22I ATGCAT 2 cut(s) 388, 1699
ErhI CCWWGG 3 cut(s) 501, 712, 1442
Esp3I CGTCTC 1 cut(s) 2023
FalI AAGNNNNNCTT 4 cut(s) 434, 466, 626, 658
FaqI GGGAC 2 cut(s) 792, 1328
FauNDI CATATG 1 cut(s) 1942
FbaI TGATCA 2 cut(s) 36, 1083
FblI GTMKAC 1 cut(s) 1627
Fnu4HI GCNGC 7 cut(s) 10, 225, 228, 231, 253, 2450, 2916
FokI GGATG 7 cut(s) 141, 659, 1262, 1349, 1559, 1793, 2615
Fsp4HI GCNGC 7 cut(s) 10, 225, 228, 231, 253, 2450, 2916
FspBI CTAG 6 cut(s) 296, 731, 872, 1017, 1923, 2685
GluI GCNGC 7 cut(s) 10, 225, 228, 231, 253, 2450, 2916
GsaI CCCAGC 4 cut(s) 1821, 1826, 1895, 2140
GsuI CTGGAG 3 cut(s) 842, 1184, 1494
HaeIII GGCC 6 cut(s) 90, 585, 1808, 2154, 2475, 2731
HapII CCGG 2 cut(s) 541, 2885
HgaI GACGC 2 cut(s) 665, 1151
Hin1I GRCGYC 1 cut(s) 2032
HincII GTYRAC 1 cut(s) 2560
HindII GTYRAC 1 cut(s) 2560
HindIII AAGCTT 3 cut(s) 640, 1219, 1661
HinfI GANTC 8 cut(s) 151, 527, 749, 838, 1100, 2011, 2609, 2888
HpaII CCGG 2 cut(s) 541, 2885
HphI GGTGA 8 cut(s) 188, 436, 471, 596, 1164, 1284, 1574, 1657
Hpy99I CGWCG 1 cut(s) 2493
HpyCH4III ACNGT 8 cut(s) 353, 519, 1670, 1761, 1874, 1918, 2090, 2264
HpyCH4IV ACGT 5 cut(s) 2032, 2488, 2674, 2775, 2795
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 1814
HpySE526I ACGT 5 cut(s) 2032, 2488, 2674, 2775, 2795
Hsp92I GRCGYC 1 cut(s) 2032
Ksp22I TGATCA 2 cut(s) 36, 1083
LguI GCTCTTC 1 cut(s) 314
LmnI GCTCC 5 cut(s) 903, 1858, 1893, 1975, 2446
Lsp1109I GCAGC 6 cut(s) 236, 239, 242, 264, 2461, 2927
LweI GCATC 2 cut(s) 640, 1540
MabI ACCWGGT 1 cut(s) 598
MaeI CTAG 6 cut(s) 296, 731, 872, 1017, 1923, 2685
MaeII ACGT 5 cut(s) 2032, 2488, 2674, 2775, 2795
MaeIII GTNAC 5 cut(s) 353, 513, 602, 1452, 2012
MflI RGATCY 3 cut(s) 1504, 1650, 2002
MlsI TGGCCA 1 cut(s) 2731
MluNI TGGCCA 1 cut(s) 2731
MlyI GAGTC 4 cut(s) 847, 1094, 2020, 2897
MmeI TCCRAC 4 cut(s) 223, 1505, 2055, 2804
Mox20I TGGCCA 1 cut(s) 2731
Mph1103I ATGCAT 2 cut(s) 388, 1699
MroXI GAANNNNTTC 3 cut(s) 576, 1550, 2232
MscI TGGCCA 1 cut(s) 2731
MslI CAYNNNNRTG 3 cut(s) 919, 1836, 1997
Msp20I TGGCCA 1 cut(s) 2731
MspCI CTTAAG 1 cut(s) 440
MspI CCGG 2 cut(s) 541, 2885
MspR9I CCNGG 6 cut(s) 542, 600, 821, 1193, 1202, 1560
Mva1269I GAATGC 1 cut(s) 856
MvaI CCWGG 5 cut(s) 600, 821, 1193, 1202, 1560
MwoI GCNNNNNNNGC 2 cut(s) 230, 1814
NciI CCSGG 1 cut(s) 542
NcoI CCATGG 2 cut(s) 501, 712
NdeI CATATG 1 cut(s) 1942
NlaIV GGNNCC 6 cut(s) 264, 448, 808, 1506, 2004, 2418
NmuCI GTSAC 3 cut(s) 602, 1452, 2012
NsiI ATGCAT 2 cut(s) 388, 1699
NspI RCATGY 4 cut(s) 307, 390, 898, 1304
PaeI GCATGC 1 cut(s) 390
PaeR7I CTCGAG 1 cut(s) 182
PceI AGGCCT 1 cut(s) 2154
PciI ACATGT 1 cut(s) 1300
PciSI GCTCTTC 1 cut(s) 314
PctI GAATGC 1 cut(s) 856
PdmI GAANNNNTTC 3 cut(s) 576, 1550, 2232
PfeI GAWTC 4 cut(s) 151, 527, 749, 2609
PflMI CCANNNNNTGG 1 cut(s) 1207
PfoI TCCNGGA 2 cut(s) 1191, 1200
PkrI GCNGC 7 cut(s) 11, 226, 229, 232, 254, 2451, 2917
PleI GAGTC 4 cut(s) 846, 1094, 2019, 2896
PmaCI CACGTG 1 cut(s) 2675
PmlI CACGTG 1 cut(s) 2675
PpsI GAGTC 4 cut(s) 846, 1094, 2019, 2896
Ppu21I YACGTR 1 cut(s) 2675
PpuMI RGGWCCY 2 cut(s) 806, 2908
PscI ACATGT 1 cut(s) 1300
PsiI TTATAA 2 cut(s) 2570, 2625
Psp5II RGGWCCY 2 cut(s) 806, 2908
Psp6I CCWGG 5 cut(s) 598, 819, 1191, 1200, 1558
PspCI CACGTG 1 cut(s) 2675
PspEI GGTNACC 1 cut(s) 602
PspFI CCCAGC 4 cut(s) 1817, 1822, 1891, 2136
PspGI CCWGG 5 cut(s) 598, 819, 1191, 1200, 1558
PspN4I GGNNCC 6 cut(s) 264, 448, 808, 1506, 2004, 2418
PspPI GGNCC 3 cut(s) 262, 806, 2908
PspPPI RGGWCCY 2 cut(s) 806, 2908
PsuI RGATCY 3 cut(s) 1504, 1650, 2002
RsaI GTAC 8 cut(s) 322, 413, 698, 1325, 1658, 1672, 2045, 2531
RsaNI GTAC 8 cut(s) 321, 412, 697, 1324, 1657, 1671, 2044, 2530
RseI CAYNNNNRTG 3 cut(s) 919, 1836, 1997
SapI GCTCTTC 1 cut(s) 314
SatI GCNGC 7 cut(s) 10, 225, 228, 231, 253, 2450, 2916
Sau96I GGNCC 3 cut(s) 262, 806, 2908
ScaI AGTACT 1 cut(s) 1672
SchI GAGTC 4 cut(s) 847, 1094, 2020, 2897
ScrFI CCNGG 6 cut(s) 542, 600, 821, 1193, 1202, 1560
SexAI ACCWGGT 1 cut(s) 598
SfaNI GCATC 2 cut(s) 640, 1540
SfcI CTRYAG 3 cut(s) 64, 522, 2223
Sfr274I CTCGAG 1 cut(s) 182
SinI GGWCC 3 cut(s) 262, 806, 2908
SlaI CTCGAG 1 cut(s) 182
SmiMI CAYNNNNRTG 3 cut(s) 919, 1836, 1997
SmlI CTYRAG 7 cut(s) 80, 182, 440, 764, 974, 1775, 2026
SmoI CTYRAG 7 cut(s) 80, 182, 440, 764, 974, 1775, 2026
SphI GCATGC 1 cut(s) 390
SseBI AGGCCT 1 cut(s) 2154
SsiI CCGC 4 cut(s) 1054, 1175, 1705, 2420
SspI AATATT 1 cut(s) 2205
SspMI CTAG 6 cut(s) 296, 731, 872, 1017, 1923, 2685
StuI AGGCCT 1 cut(s) 2154
StyD4I CCNGG 6 cut(s) 540, 598, 819, 1191, 1200, 1558
StyI CCWWGG 3 cut(s) 501, 712, 1442
TaaI ACNGT 8 cut(s) 353, 519, 1670, 1761, 1874, 1918, 2090, 2264
TaiI ACGT 5 cut(s) 2035, 2491, 2677, 2778, 2798
TaqI TCGA 4 cut(s) 183, 1767, 1953, 2241
TatI WGTACW 4 cut(s) 320, 696, 1670, 2529
TfiI GAWTC 4 cut(s) 151, 527, 749, 2609
TscAI CASTG 9 cut(s) 100, 145, 776, 1045, 1053, 1439, 2267, 2311, 2863
TseFI GTSAC 3 cut(s) 602, 1452, 2012
TseI GCWGC 7 cut(s) 9, 224, 227, 230, 252, 2449, 2915
Tsp45I GTSAC 3 cut(s) 602, 1452, 2012
TspGWI ACGGA 2 cut(s) 384, 1565
TspRI CASTG 9 cut(s) 100, 145, 776, 1045, 1053, 1439, 2267, 2311, 2863
Van91I CCANNNNNTGG 1 cut(s) 1207
Vha464I CTTAAG 1 cut(s) 440
VpaK11BI GGWCC 3 cut(s) 262, 806, 2908
XagI CCTNNNNNAGG 1 cut(s) 2765
XapI RAATTY 6 cut(s) 617, 1276, 1908, 1927, 2167, 2216
XbaI TCTAGA 1 cut(s) 295
XceI RCATGY 4 cut(s) 307, 390, 898, 1304
XhoI CTCGAG 1 cut(s) 182
XmiI GTMKAC 1 cut(s) 1627
XmnI GAANNNNTTC 3 cut(s) 576, 1550, 2232
XspI CTAG 6 cut(s) 296, 731, 872, 1017, 1923, 2685
ZraI GACGTC 1 cut(s) 2033
ZrmI AGTACT 1 cut(s) 1672
Zsp2I ATGCAT 2 cut(s) 388, 1699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.