RchiOBHm_Chr5g0073061

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000183
Physical Location & Seq
Reverse (-)
79116293 .. 79124327
8035 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34797

Sequence Viewer

Length: 3147 bp
ATGGGTATGGGTGTGATGGGTTGCTGCACTTTCTCGTCGGACGACATTGTAATAAAGTCTCCTAATGACAAACGACTCTACAGAGTGATTAAGCTTGAGAATGGCCTTACTGCATTGCTCATTCACGATCCTCAGGTCTACCCACAAGGACCACCCCACCACTCCTCCAACCTTGAACAAGAAGAAGAAGAAGATTGTGAAGCTGTAGAAGAAGAAAAGGAAGAGGAAGGTGATGTTAAAAGGAAGGAAGAGGGAGGTGCTTCTGAGACTAAGAAGGCAGCAGCAGCAATGTGTGTCGGAATAGGCAGCTTCTCTGACCCTCTCGAGGCTCAGGGGCTTGCACATTTTCTAGAACACATGCTGTTCATGGGGAGTACAAAGTTCCCAGATGAAAATGAGTATGCTAGTTACTTGTCCAAGCATGGAGGCTCGTCAAATGCATACACAGAAGCAGAGCATACTTGTTACTACTTTGATGTGAAACGAGAATTTCTTAAGGGTGCCTTGGAAAGATTTTCTCAGTTCTTTGTTTCACCTCTAATGAAAAGTGAAGCCATGGCAAGAGAGGTACAGGCTATAGATTCAGAGTTTAATACGGTTCTGCAAAACGATTTTTGCCGCCTTCAACAACTTCAAGGCCATACATCCTCACCTGGTCACCCATTTAATAAATTTTCTTGGGGAAATAAGAAGAGCTTGGATGATGCAAAGAAAAAAGGGATCAACTTGCAGGAACAAATACTTAAATTGTACAGAGACAATTACCACGGTGGATTAATGAAGCTAGTTGTCATTGGTGGAGAATCTCTCGATGTACTTGAGCACTGGGTTTCGGAATTGTTTGGAGATGTCAAAAAAGATCCCCAAGTAAATCTGGAGTTCAAGGCAGAAGGTCCTATTTGGAAAGCTGGAAAACTTTACAGGCTAGAGGCTGTTGACGATGTTCATATACTCCACCTAGCATGGACACTTCCATGTCTTCAAGAACACTATTTGAAGAAACCAGAAGATTACTTGAGTCATCTGCTTGGGCATGAGGGCAGGGGAAGTTTGCATTTCTACCTGAAAGCTAGAGGGTGGGTAACATCTTTAGATACTTGTTTGAGCGGGATGGACTGCTCTTCTGTGGCTTATATCTTTTGCATGGTCATATACCTCACTGACTCTGGATTGGAGAAGATGTTTGAAATAATTGGATTTGTGTATCAATACATTAAGTTATTGAGTCAAATGTCACCACAAGAGTGGATATTCCGGGAACTTCAGGATATTGGGAACATGGACTTTAGATTTGCAGAGGAGCAGGACCAGGATGATTATGCTTCACAACTTGCAGAAAATTTACTATATTATGCAACAGAGCATGTTATTTATGGGGACTATGTGAACGAGAGTTGGGATAAGGAATTGATAGAATATGTTCTTGGTTTCTTCAGACCAGAAAACATGAGGATTGATGTGATATCAAAGTCCTTATTTAAGTCAGAAGATTTCCAGTGTGAGCCTTGGTTTGGATCACATTATACTGAGGAAGATATATCTCCATCTTTAATAGATTTATGGAAGAATCCTCAAGAAATTAATGTTTCATTGCATCTGCCGGCAAAGAATGATTTCATTCCTCGTGATTTCTCCATCCGTTCTGATGTTTTGTGCATTGATACTGCAACGACATCTTATCCAAAATGTATACTTGATGAACCATTGATGAAGTTTTGGTACAAGCTCGACAGTACATTTAAACTTCCACATGTAAATACATACTTCAGCATCAATCTGAAGGGTGCATATGATGATGTGAAGAGTTGTGTTTTGACTAGCTTATACCTTGACCTTCTCACAGATCAGCTGAATGAGATCCTCTATGAGGCCTGTGTTGCCAGCCTGGGAACTTCTTTTTCTCTGTCTCTGGACAAACTGCAGTTAGAGGTATGCGGTTTCAATGATAAGCTTCCAGCTCTGTTATCAAAAATTTTGGAAACAATAAAAAGTTTCTTGCCAACTGATGATCGTTTTGAGGTTTATAAAGAAGATATGGAGCGAGCATATATGAACACCAATATGGATCCTTGGAGTTACTCAACATACTTGAGAGATCAAGTTCTGTTGCAGAAATTCTATACCGTAGATGAGCAGTTGCATGTTTTGAAGGGATTGTCTGTTTCTGATCTGAAGTCTTTCATTCCTGAGATTTTTTCCCAGGCATGGCCTTTTGCATGGCAATTGTCAGAAGAAGAAACAATTAATCTTGCAAAATTAATTCAAACAAACTTTACTGTACCAGCACTTCCTACCAAATTGGTGTATAAAGACCATTGTATCTGTCTTCCTCCAAATGCTAACCTCGTCAGAGATGCTACTGTGAAGAACAAGTCAGAAACAAACTCTGTGACTGAGTTGTATTTTCAAATTGAGCAGGCAGTGAAGAGTGAGTCCGTCAGACTAAAAGTATTGATTGACCTTTTTCAGGAAATTGTACAGGAACCACTTTTTAATCAACTAAGGACGAAGGAGCAGCTTGGTTATGTTGTTCTCTGTGGCCAGAATTGTACGTGCAATGTTTTTGGCTTCTATTTCTGTGTTCAGTCATCCGAGTACAACCCGATCCACCTTCAAGGCAGACTGGACAACTTTATAGATGGTCTGGAAGAGTTGTTGGAAGGGCTGGACGATGATTCCTTTGAGAATTATAAAGGTGGACTAATGGCCAAGGTTTTGGAGAAAGATGCATACCTCACATGTGAAACCAATCGATTGTGGACTCAGATTCTTGATAAATGGTATACGTTTGACTGTTCGAAAAAGGCCGCAGAACAACTCAGAAGCATTCAGAAGGAGGATGTTATCAACTTTTACAAGACCTATTTGCAACAATCATCTCCAAAGCGTCGAAGACTTGCAACTCGTGTATGGGGTTGCAATCAGCGTTTTAAGAGGCGACTGCATGAGGCGAGGCGTTTTGCCCGAGCATTAAGCCTCAAAAGCCTTAAGGCGCTTTTAATGTGCAAGTCATTGAAGACCTTGCAAGAAGCTGAAGAAGCACGACCGGAGTCTGTGCAAGTCATTGAAGACCTTGCAGCCTTTAAGATGTCGTCCAAGTTTTATGGTTGTAAAAGATGTCAAAAAGTTACTCCTCTGAAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

1048

Amino Acids

120.66

Weight (kDa)

5.18

Isoelectric Point (pI)

44.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 89 - 218 3e-34 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 245 - 422 1.9e-17 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 429 - 714 1.9e-86 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 778 - 899 6.7e-13 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 821 - 920 7.5e-23 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2025, 2691
AccB1I GGYRCC 1 cut(s) 500
AccBSI CCGCTC 1 cut(s) 1107
AccI GTMKAC 3 cut(s) 138, 1690, 2783
AciI CCGC 4 cut(s) 619, 1107, 1935, 2808
AclWI GGATC 8 cut(s) 122, 728, 854, 1522, 1852, 2060, 2073, 2599
AcoI YGGCCR 2 cut(s) 2539, 2706
AcsI RAATTY 5 cut(s) 488, 671, 1339, 1971, 2114
AcuI CTGAAG 6 cut(s) 1247, 1417, 1750, 1799, 2192, 3054
AfiI CCNNNNNNNGG 5 cut(s) 325, 1511, 1867, 2205, 2467
AflII CTTAAG 2 cut(s) 494, 2987
AflIII ACRYGT 2 cut(s) 1750, 2738
AjnI CCWGG 4 cut(s) 652, 1308, 1884, 2199
AjuI GAANNNNNNNTTGG 2 cut(s) 1407, 1439
AleI CACNNNNGTG 1 cut(s) 1243
Alw21I GWGCWC 1 cut(s) 825
Alw26I GTCTC 4 cut(s) 63, 260, 750, 1911
AlwI GGATC 8 cut(s) 122, 728, 854, 1522, 1852, 2060, 2073, 2599
Ama87I CYCGRG 2 cut(s) 323, 2964
AoxI GGCC 7 cut(s) 103, 637, 1869, 2207, 2539, 2706, 2805
ApeKI GCWGC 7 cut(s) 24, 278, 281, 284, 306, 2515, 3077
ApoI RAATTY 5 cut(s) 488, 671, 1339, 1971, 2114
ArsI GACNNNNNNTTYG 2 cut(s) 1274, 1306
AseI ATTAAT 4 cut(s) 776, 1581, 2244, 2258
Asp700I GAANNNNTTC 3 cut(s) 1419, 1613, 2177
AspLEI GCGC 1 cut(s) 2995
AspS9I GGNCC 3 cut(s) 149, 893, 1306
AsuC2I CCSGG 1 cut(s) 1256
AsuHPI GGTGA 5 cut(s) 242, 525, 642, 650, 1227
AsuII TTCGAA 1 cut(s) 2798
AvaI CYCGRG 2 cut(s) 323, 2964
AvaII GGWCC 3 cut(s) 149, 893, 1306
AxyI CCTNAGG 1 cut(s) 132
BaeI ACNNNNGTAYC 2 cut(s) 2302, 2335
BalI TGGCCA 2 cut(s) 2541, 2708
BamHI GGATCC 1 cut(s) 2065
BanI GGYRCC 1 cut(s) 500
BarI GAAGNNNNNNTAC 4 cut(s) 1703, 1735, 2271, 2303
BauI CACGAG 2 cut(s) 1623, 2904
BbsI GAAGAC 5 cut(s) 971, 2318, 2899, 3023, 3075
Bbv12I GWGCWC 1 cut(s) 825
BbvI GCAGC 7 cut(s) 11, 290, 293, 296, 318, 2527, 3089
BccI CCATC 5 cut(s) 10, 1105, 1552, 1643, 2633
BciT130I CCWGG 4 cut(s) 654, 1310, 1886, 2201
BcnI CCSGG 1 cut(s) 1256
BcoDI GTCTC 4 cut(s) 63, 260, 750, 1911
BfaI CTAG 7 cut(s) 350, 405, 785, 926, 959, 1071, 1818
BfmI CTRYAG 4 cut(s) 79, 204, 576, 1919
BfoI RGCGCY 1 cut(s) 2996
BfrI CTTAAG 2 cut(s) 494, 2987
BisI GCNGC 9 cut(s) 25, 279, 282, 285, 307, 619, 2516, 2808, 3078
BlsI GCNGC 9 cut(s) 26, 280, 283, 286, 308, 620, 2517, 2809, 3079
Bme1390I CCNGG 5 cut(s) 654, 1256, 1310, 1886, 2201
Bme18I GGWCC 3 cut(s) 149, 893, 1306
BmeT110I CYCGRG 2 cut(s) 323, 2964
BmgT120I GGNCC 3 cut(s) 149, 893, 1306
BmiI GGNNCC 3 cut(s) 502, 2067, 2484
BmrFI CCNGG 5 cut(s) 654, 1256, 1310, 1886, 2201
BmrI ACTGGG 1 cut(s) 835
BmsI GCATC 5 cut(s) 694, 1603, 1779, 2344, 2716
BmuI ACTGGG 1 cut(s) 835
BoxI GACNNNNGTC 1 cut(s) 3049
BpiI GAAGAC 5 cut(s) 971, 2318, 2899, 3023, 3075
BplI GAGNNNNNCTC 2 cut(s) 792, 824
BpmI CTGGAG 1 cut(s) 896
Bpu10I CCTNAGC 1 cut(s) 330
Bpu14I TTCGAA 1 cut(s) 2798
BpuEI CTTGAG 5 cut(s) 116, 839, 1036, 1557, 2110
BpuMI CCSGG 1 cut(s) 1256
Bsa29I ATCGAT 1 cut(s) 2752
BsaAI YACGTR 1 cut(s) 2553
BsaJI CCNNGG 8 cut(s) 504, 555, 766, 1505, 1885, 2069, 2199, 2709
BsaWI WCCGGW 1 cut(s) 3046
BsaXI ACNNNNNCTCC 4 cut(s) 149, 179, 1166, 1196
Bsc4I CCNNNNNNNGG 5 cut(s) 325, 1511, 1867, 2205, 2467
Bse118I RCCGGY 1 cut(s) 1600
Bse1I ACTGG 3 cut(s) 830, 1495, 2628
Bse21I CCTNAGG 1 cut(s) 132
Bse3DI GCAATG 4 cut(s) 113, 294, 1589, 2563
BseBI CCWGG 4 cut(s) 654, 1310, 1886, 2201
BseCI ATCGAT 1 cut(s) 2752
BseDI CCNNGG 8 cut(s) 504, 555, 766, 1505, 1885, 2069, 2199, 2709
BseGI GGATG 7 cut(s) 644, 706, 1116, 1318, 1635, 2588, 2845
BseLI CCNNNNNNNGG 5 cut(s) 325, 1511, 1867, 2205, 2467
BseMI GCAATG 4 cut(s) 113, 294, 1589, 2563
BseMII CTCAG 9 cut(s) 146, 255, 344, 533, 1518, 2178, 2385, 2777, 2833
BseNI ACTGG 3 cut(s) 830, 1495, 2628
BseRI GAGGAG 3 cut(s) 154, 1313, 3123
BseXI GCAGC 7 cut(s) 11, 290, 293, 296, 318, 2527, 3089
BsgI GTGCAG 1 cut(s) 10
Bsh1285I CGRYCG 1 cut(s) 3047
BshFI GGCC 7 cut(s) 105, 639, 1871, 2209, 2541, 2708, 2807
BshNI GGYRCC 1 cut(s) 500
BshVI ATCGAT 1 cut(s) 2752
BsiEI CGRYCG 1 cut(s) 3047
BsiHKAI GWGCWC 1 cut(s) 825
BsiHKCI CYCGRG 2 cut(s) 323, 2964
BsiSI CCGG 3 cut(s) 1255, 1601, 3047
BslFI GGGAC 1 cut(s) 1391
BslI CCNNNNNNNGG 5 cut(s) 325, 1511, 1867, 2205, 2467
BsmAI GTCTC 4 cut(s) 63, 260, 750, 1911
BsmFI GGGAC 1 cut(s) 1391
BsmI GAATGC 1 cut(s) 2826
BsnI GGCC 7 cut(s) 105, 639, 1871, 2209, 2541, 2708, 2807
BsoBI CYCGRG 2 cut(s) 323, 2964
Bsp119I TTCGAA 1 cut(s) 2798
Bsp1286I GDGCHC 1 cut(s) 825
Bsp1407I TGTACA 2 cut(s) 750, 2476
Bsp19I CCATGG 1 cut(s) 555
BspACI CCGC 4 cut(s) 619, 1107, 1935, 2808
BspANI GGCC 7 cut(s) 105, 639, 1871, 2209, 2541, 2708, 2807
BspCNI CTCAG 9 cut(s) 145, 256, 343, 532, 1519, 2179, 2386, 2776, 2832
BspDI ATCGAT 1 cut(s) 2752
BspLI GGNNCC 3 cut(s) 502, 2067, 2484
BspMAI CTGCAG 1 cut(s) 1923
BspPI GGATC 8 cut(s) 122, 728, 854, 1522, 1852, 2060, 2073, 2599
BspQI GCTCTTC 2 cut(s) 686, 1126
BspT104I TTCGAA 1 cut(s) 2798
BspT107I GGYRCC 1 cut(s) 500
BspTI CTTAAG 2 cut(s) 494, 2987
BsrBI CCGCTC 1 cut(s) 1107
BsrDI GCAATG 4 cut(s) 113, 294, 1589, 2563
BsrFI RCCGGY 1 cut(s) 1600
BsrGI TGTACA 2 cut(s) 750, 2476
BsrI ACTGG 3 cut(s) 830, 1495, 2628
BssAI RCCGGY 1 cut(s) 1600
BssECI CCNNGG 8 cut(s) 504, 555, 766, 1505, 1885, 2069, 2199, 2709
BssNAI GTATAC 2 cut(s) 1691, 2784
BssSI CACGAG 2 cut(s) 1623, 2904
BssT1I CCWWGG 5 cut(s) 504, 555, 1505, 2069, 2709
Bst1107I GTATAC 2 cut(s) 1691, 2784
Bst2BI CACGAG 2 cut(s) 1623, 2904
Bst2UI CCWGG 4 cut(s) 654, 1310, 1886, 2201
Bst4CI ACNGT 7 cut(s) 598, 770, 1733, 2125, 2278, 2362, 2795
Bst6I CTCTTC 7 cut(s) 216, 243, 686, 1126, 1796, 2420, 2643
BstAFI CTTAAG 2 cut(s) 494, 2987
BstAUI TGTACA 2 cut(s) 750, 2476
BstBAI YACGTR 1 cut(s) 2553
BstBI TTCGAA 1 cut(s) 2798
BstC8I GCNNGC 5 cut(s) 339, 1602, 1882, 2043, 2418
BstDSI CCRYGG 2 cut(s) 555, 766
BstEII GGTNACC 1 cut(s) 656
BstENI CCTNNNNNAGG 2 cut(s) 1865, 2465
BstF5I GGATG 7 cut(s) 644, 706, 1116, 1318, 1635, 2588, 2845
BstH2I RGCGCY 1 cut(s) 2996
BstHHI GCGC 1 cut(s) 2995
BstMAI GTCTC 4 cut(s) 63, 260, 750, 1911
BstMCI CGRYCG 1 cut(s) 3047
BstMWI GCNNNNNNNGC 4 cut(s) 284, 1877, 2982, 3038
BstNI CCWGG 4 cut(s) 654, 1310, 1886, 2201
BstNSI RCATGY 5 cut(s) 361, 1367, 1754, 2144, 2742
BstPAI GACNNNNGTC 1 cut(s) 3049
BstPI GGTNACC 1 cut(s) 656
BstSCI CCNGG 5 cut(s) 652, 1254, 1308, 1884, 2199
BstSFI CTRYAG 4 cut(s) 79, 204, 576, 1919
BstV1I GCAGC 7 cut(s) 11, 290, 293, 296, 318, 2527, 3089
BstV2I GAAGAC 5 cut(s) 971, 2318, 2899, 3023, 3075
BstX2I RGATCY 3 cut(s) 859, 1857, 2065
BstXI CCANNNNNNTGG 2 cut(s) 1245, 2716
BstYI RGATCY 3 cut(s) 859, 1857, 2065
BstZ17I GTATAC 2 cut(s) 1691, 2784
Bsu15I ATCGAT 1 cut(s) 2752
Bsu36I CCTNAGG 1 cut(s) 132
BsuRI GGCC 7 cut(s) 105, 639, 1871, 2209, 2541, 2708, 2807
BsuTUI ATCGAT 1 cut(s) 2752
BtgI CCRYGG 2 cut(s) 555, 766
BtsCI GGATG 7 cut(s) 644, 706, 1116, 1318, 1635, 2588, 2845
BtsI GCAGTG 1 cut(s) 2427
BtsIMutI CAGTG 4 cut(s) 823, 1158, 1502, 2427
Cac8I GCNNGC 5 cut(s) 339, 1602, 1882, 2043, 2418
CfoI GCGC 1 cut(s) 2995
Cfr10I RCCGGY 1 cut(s) 1600
Cfr13I GGNCC 3 cut(s) 149, 893, 1306
ClaI ATCGAT 1 cut(s) 2752
CseI GACGC 1 cut(s) 2876
CsiI ACCWGGT 1 cut(s) 652
DraI TTTAAA 1 cut(s) 1741
EaeI YGGCCR 2 cut(s) 2539, 2706
Eam1104I CTCTTC 7 cut(s) 216, 243, 686, 1126, 1796, 2420, 2643
EarI CTCTTC 7 cut(s) 216, 243, 686, 1126, 1796, 2420, 2643
Eco130I CCWWGG 5 cut(s) 504, 555, 1505, 2069, 2709
Eco147I AGGCCT 1 cut(s) 1871
Eco32I GATATC 1 cut(s) 1464
Eco47I GGWCC 3 cut(s) 149, 893, 1306
Eco57I CTGAAG 6 cut(s) 1247, 1417, 1750, 1799, 2192, 3054
Eco81I CCTNAGG 1 cut(s) 132
Eco88I CYCGRG 2 cut(s) 323, 2964
Eco91I GGTNACC 1 cut(s) 656
EcoNI CCTNNNNNAGG 2 cut(s) 1865, 2465
EcoO109I RGGNCCY 1 cut(s) 893
EcoO65I GGTNACC 1 cut(s) 656
EcoRII CCWGG 4 cut(s) 652, 1308, 1884, 2199
EcoRV GATATC 1 cut(s) 1464
EcoT14I CCWWGG 5 cut(s) 504, 555, 1505, 2069, 2709
EcoT22I ATGCAT 2 cut(s) 442, 2731
ErhI CCWWGG 5 cut(s) 504, 555, 1505, 2069, 2709
FalI AAGNNNNNCTT 4 cut(s) 488, 520, 680, 712
FaqI GGGAC 1 cut(s) 1391
FauI CCCGC 1 cut(s) 1100
FauNDI CATATG 1 cut(s) 1789
FblI GTMKAC 3 cut(s) 138, 1690, 2783
Fnu4HI GCNGC 9 cut(s) 25, 279, 282, 285, 307, 619, 2516, 2808, 3078
FokI GGATG 7 cut(s) 631, 713, 1123, 1325, 1622, 2575, 2852
Fsp4HI GCNGC 9 cut(s) 25, 279, 282, 285, 307, 619, 2516, 2808, 3078
FspBI CTAG 7 cut(s) 350, 405, 785, 926, 959, 1071, 1818
GlaI GCGC 1 cut(s) 2994
GluI GCNGC 9 cut(s) 25, 279, 282, 285, 307, 619, 2516, 2808, 3078
GsuI CTGGAG 1 cut(s) 896
HaeII RGCGCY 1 cut(s) 2996
HaeIII GGCC 7 cut(s) 105, 639, 1871, 2209, 2541, 2708, 2807
HapII CCGG 3 cut(s) 1255, 1601, 3047
HgaI GACGC 1 cut(s) 2876
HhaI GCGC 1 cut(s) 2995
Hin6I GCGC 1 cut(s) 2993
HinP1I GCGC 1 cut(s) 2993
HincII GTYRAC 1 cut(s) 937
HindII GTYRAC 1 cut(s) 937
HindIII AAGCTT 2 cut(s) 92, 1949
HpaII CCGG 3 cut(s) 1255, 1601, 3047
HphI GGTGA 5 cut(s) 242, 525, 642, 650, 1227
Hpy166II GTNNAC 7 cut(s) 139, 937, 1387, 1691, 2699, 2760, 2784
Hpy8I GTNNAC 7 cut(s) 139, 937, 1387, 1691, 2699, 2760, 2784
Hpy99I CGWCG 2 cut(s) 40, 2892
HpyCH4III ACNGT 7 cut(s) 598, 770, 1733, 2125, 2278, 2362, 2795
HpyCH4IV ACGT 2 cut(s) 2552, 2786
HpyF10VI GCNNNNNNNGC 4 cut(s) 284, 1877, 2982, 3038
HpySE526I ACGT 2 cut(s) 2552, 2786
HspAI GCGC 1 cut(s) 2993
KroI GCCGGC 1 cut(s) 1600
KroNI GCCGGC 1 cut(s) 1602
LguI GCTCTTC 2 cut(s) 686, 1126
LmnI GCTCC 3 cut(s) 1300, 2038, 2512
Lsp1109I GCAGC 7 cut(s) 11, 290, 293, 296, 318, 2527, 3089
LweI GCATC 5 cut(s) 694, 1603, 1779, 2344, 2716
MabI ACCWGGT 1 cut(s) 652
MaeI CTAG 7 cut(s) 350, 405, 785, 926, 959, 1071, 1818
MaeII ACGT 2 cut(s) 2552, 2786
MaeIII GTNAC 8 cut(s) 407, 464, 656, 1081, 1233, 2075, 2389, 3127
MbiI CCGCTC 1 cut(s) 1107
MfeI CAATTG 1 cut(s) 2222
MflI RGATCY 3 cut(s) 859, 1857, 2065
MhlI GDGCHC 1 cut(s) 825
MlsI TGGCCA 2 cut(s) 2541, 2708
MluNI TGGCCA 2 cut(s) 2541, 2708
MlyI GAGTC 7 cut(s) 69, 1027, 1157, 1234, 2441, 2755, 3059
MmeI TCCRAC 4 cut(s) 18, 192, 277, 2637
Mox20I TGGCCA 2 cut(s) 2541, 2708
Mph1103I ATGCAT 2 cut(s) 442, 2731
MroNI GCCGGC 1 cut(s) 1600
MroXI GAANNNNTTC 3 cut(s) 1419, 1613, 2177
MscI TGGCCA 2 cut(s) 2541, 2708
MslI CAYNNNNRTG 3 cut(s) 973, 1243, 2060
Msp20I TGGCCA 2 cut(s) 2541, 2708
MspA1I CMGCKG 1 cut(s) 1849
MspCI CTTAAG 2 cut(s) 494, 2987
MspI CCGG 3 cut(s) 1255, 1601, 3047
MspR9I CCNGG 5 cut(s) 654, 1256, 1310, 1886, 2201
MunI CAATTG 1 cut(s) 2222
Mva1269I GAATGC 1 cut(s) 2826
MvaI CCWGG 4 cut(s) 654, 1310, 1886, 2201
MwoI GCNNNNNNNGC 4 cut(s) 284, 1877, 2982, 3038
NaeI GCCGGC 1 cut(s) 1602
NciI CCSGG 1 cut(s) 1256
NcoI CCATGG 1 cut(s) 555
NdeI CATATG 1 cut(s) 1789
NgoMIV GCCGGC 1 cut(s) 1600
NlaIV GGNNCC 3 cut(s) 502, 2067, 2484
NmuCI GTSAC 3 cut(s) 656, 1233, 2389
NsiI ATGCAT 2 cut(s) 442, 2731
NspI RCATGY 5 cut(s) 361, 1367, 1754, 2144, 2742
NspV TTCGAA 1 cut(s) 2798
OliI CACNNNNGTG 1 cut(s) 1243
PaeR7I CTCGAG 1 cut(s) 323
PceI AGGCCT 1 cut(s) 1871
PciI ACATGT 2 cut(s) 1750, 2738
PciSI GCTCTTC 2 cut(s) 686, 1126
PctI GAATGC 1 cut(s) 2826
PdiI GCCGGC 1 cut(s) 1602
PdmI GAANNNNTTC 3 cut(s) 1419, 1613, 2177
PfeI GAWTC 5 cut(s) 581, 803, 1567, 2675, 2767
PfoI TCCNGGA 1 cut(s) 1254
PkrI GCNGC 9 cut(s) 26, 280, 283, 286, 308, 620, 2517, 2809, 3079
PleI GAGTC 7 cut(s) 69, 1026, 1157, 1233, 2440, 2755, 3058
PpsI GAGTC 7 cut(s) 69, 1026, 1157, 1233, 2440, 2755, 3058
Ppu21I YACGTR 1 cut(s) 2553
PpuMI RGGWCCY 1 cut(s) 893
PscI ACATGT 2 cut(s) 1750, 2738
PshAI GACNNNNGTC 1 cut(s) 3049
PshBI ATTAAT 4 cut(s) 776, 1581, 2244, 2258
PsiI TTATAA 2 cut(s) 2025, 2691
Psp5II RGGWCCY 1 cut(s) 893
Psp6I CCWGG 4 cut(s) 652, 1308, 1884, 2199
PspEI GGTNACC 1 cut(s) 656
PspGI CCWGG 4 cut(s) 652, 1308, 1884, 2199
PspN4I GGNNCC 3 cut(s) 502, 2067, 2484
PspPI GGNCC 3 cut(s) 149, 893, 1306
PspPPI RGGWCCY 1 cut(s) 893
PstI CTGCAG 1 cut(s) 1923
PsuI RGATCY 3 cut(s) 859, 1857, 2065
PvuII CAGCTG 1 cut(s) 1849
RseI CAYNNNNRTG 3 cut(s) 973, 1243, 2060
SapI GCTCTTC 2 cut(s) 686, 1126
SatI GCNGC 9 cut(s) 25, 279, 282, 285, 307, 619, 2516, 2808, 3078
Sau96I GGNCC 3 cut(s) 149, 893, 1306
SchI GAGTC 7 cut(s) 69, 1027, 1157, 1234, 2441, 2755, 3059
ScrFI CCNGG 5 cut(s) 654, 1256, 1310, 1886, 2201
SduI GDGCHC 1 cut(s) 825
SexAI ACCWGGT 1 cut(s) 652
SfaNI GCATC 5 cut(s) 694, 1603, 1779, 2344, 2716
SfcI CTRYAG 4 cut(s) 79, 204, 576, 1919
Sfr274I CTCGAG 1 cut(s) 323
SfuI TTCGAA 1 cut(s) 2798
SinI GGWCC 3 cut(s) 149, 893, 1306
SlaI CTCGAG 1 cut(s) 323
SmiMI CAYNNNNRTG 3 cut(s) 973, 1243, 2060
SmlI CTYRAG 8 cut(s) 95, 323, 494, 818, 1015, 1572, 2089, 2987
SmoI CTYRAG 8 cut(s) 95, 323, 494, 818, 1015, 1572, 2089, 2987
SseBI AGGCCT 1 cut(s) 1871
SsiI CCGC 4 cut(s) 619, 1107, 1935, 2808
SspMI CTAG 7 cut(s) 350, 405, 785, 926, 959, 1071, 1818
StuI AGGCCT 1 cut(s) 1871
StyD4I CCNGG 5 cut(s) 652, 1254, 1308, 1884, 2199
StyI CCWWGG 5 cut(s) 504, 555, 1505, 2069, 2709
TaaI ACNGT 7 cut(s) 598, 770, 1733, 2125, 2278, 2362, 2795
TaiI ACGT 2 cut(s) 2555, 2789
TaqI TCGA 6 cut(s) 324, 810, 1728, 2752, 2798, 2890
TatI WGTACW 6 cut(s) 374, 750, 814, 1733, 2476, 2595
TauI GCSGC 2 cut(s) 621, 2810
TfiI GAWTC 5 cut(s) 581, 803, 1567, 2675, 2767
TscAI CASTG 4 cut(s) 830, 1165, 1502, 2427
TseFI GTSAC 3 cut(s) 656, 1233, 2389
TseI GCWGC 7 cut(s) 24, 278, 281, 284, 306, 2515, 3077
Tsp45I GTSAC 3 cut(s) 656, 1233, 2389
TspGWI ACGGA 2 cut(s) 1628, 2425
TspRI CASTG 4 cut(s) 830, 1165, 1502, 2427
Vha464I CTTAAG 2 cut(s) 494, 2987
VpaK11BI GGWCC 3 cut(s) 149, 893, 1306
VspI ATTAAT 4 cut(s) 776, 1581, 2244, 2258
XagI CCTNNNNNAGG 2 cut(s) 1865, 2465
XapI RAATTY 5 cut(s) 488, 671, 1339, 1971, 2114
XbaI TCTAGA 1 cut(s) 349
XceI RCATGY 5 cut(s) 361, 1367, 1754, 2144, 2742
XhoI CTCGAG 1 cut(s) 323
XmiI GTMKAC 3 cut(s) 138, 1690, 2783
XmnI GAANNNNTTC 3 cut(s) 1419, 1613, 2177
XspI CTAG 7 cut(s) 350, 405, 785, 926, 959, 1071, 1818
Zsp2I ATGCAT 2 cut(s) 442, 2731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.