Rroxscaffold_3G00254700

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000183
Physical Location & Seq
Reverse (-)
48991776 .. 48999348
7573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00254700.1

Sequence Viewer

Length: 2739 bp
ATGGGTCGCAGCAATTTTTCATCGGACGACATTGTGATCAAGTCTCCTAATGATAACAAACTTTACAGATTGATTACGCTTGAGAATGGCCTCACTGCATTGATCCTTCACGATCCTGAGATTTACCCACAACCCTCCACTGAAGCTCAGATTGATCCATTGCTAATTCAACAAAATGGTGAACTCAGGAGCAAGGAAAAGGGAGGTGCTTTTCAGACTAAGAAGGCAGCAGCAGCAATGTTTGTTGGAATGGGCAGCTTCTCTGACCCTCCTGAGGCACAGGGGCTTGCACACTTTCTAGAACACATGCTCTTCATGGGGAGTACAGCGTTTCCAGATGAAAATGAGTATGACAGTTACTTGTCTAAGCACGGAGGGTCATCAAATGCATGCACTCGTGAAGAGAATACTTCGTACCACTTTGAGGTGAAACCAGAGTTTCTTAAGGGTGCCTTGTCAAGTACTGCAACATACAATTTCCTTATCGCTGAATTGTATTCAAAATGCAATGCAGAGTTCAACCGGGTTCTGCAAAACGATTTTTGCCGCCTTGAACAACTTCAAGGCCATACAGCCACACCTGGTCACCCATTTAATAAATTCACTTGGGGAAATAAGAAAAGCTTGGATGATGCAAGGGAAAAGGGGATAAACTTGCGTCAACAAATACTGAAATTGTACAGAGACTATTACCATGGTGGATTGATGAAGCTAGTTGTCATTGGTGGAGAATCTCTTGATATACTTGAGCAGTGGGTTCTGGAATTGTTTGGAGATATCAAAAAAGGTCCCCGAGTAAACCTGGAGTTCAAGGCAGAGGGTCCTATTTGGAATGCTGGAAAACTTTACAGGCTAGAGGCTGTTAATGATGTTCACATGCTCCACTTAGCATGGACACTTCCATGTCTTAATGAACACTATTTGAAAAGCCCAGATTATTTTTTACGTCATCTCCTTGAGCATGAGGGCAGGGGAAGTCTGTATTTTTATCTCAAAGCTAGAGGGTGGGCAACATTTCTCAGTGCTTCGGTGAACCGCAGTTCTGTGGCTGATGTCTTTTGCATGATCATATACCTTACTGACTCTGGATTGGAGCAGATTTTTGAGATAATTGGGTTGGTGTATCAATACATTAAATTATTGCGTGAAGTATCACCGCAAGAATGGATATTCCAGGAACTCCAGGATATTGGGAACATGAGCTTTAGATTTTTACAGGATCAACCCCAGGATGAATATGCTGTAAAACTTGGAGGAAATTTACTACGTTATGCACCAGAACATGTTATTTATGGGGACTATGTGTACGAGACTTGGGATGAGGAATTGATAAAATATGTTCTTGGTTTCTTCAGACCAGAAAACATGAGGATTGATGTGATATCAAAGTCCTCATTTAAGTCAGAAGCAGTAAAACAGTGTGGTCAAGCACTAACATGTCCTTCAATTGTTTCAGATTTCCAGTGCGAGCCTTGGTTTGGGTCACATTATACTGAAGAAGATATATCTCCAACTTTGATGAAGTTGTGGAAGGATCCTCCAGAAGTTGATATTTCATTGCATCTCCCTCAAAAGAATATGTTCATTCCTGGTGATTTCTCCATTCGTTCAGATGTTTGTTGTCCTGATATTGCAATTATATCTTCTCCAAGATGTATACTTGATGAACCATTGGTGAAGATCTGGTACAAGCTTGACAGTACTTTTAAACAACCACGGGTAAATGCATACTTCCGCATAAATCTGAAGGGTGGATATGATAATGTCAAGAGTTATGTTTTGACTGAACTGTATATCGAGCTTCTTGAGGATGAGCTGAATGAGATCATCTATCAGGCCAATGTTGCTGGGCTAGGAACTCATGTATATGGGTCTAACGACTTTATGGAGCTGAAGGTTTATGGTTTCAATGATAAGCTTCCAGCTTTATTGTCAAAAATTCTGACAGTAGCTAGAAATTTCTTGCCAACATATGATCGTTTCAAGGTTATTAAAGAAAGAATGGAGCGAATGTTAAAGAACACTAATATGGATCCTCTGAGTCACTCTTCATACTTGAGACGTCAAGTTTTGTACCGTAGATACTATGATGTAGATGAAGAGTTGCAAGTCTTAAACAGTCTGTCTGTTTCTGATATGAAGTCATTTATTCCTGAGCTTTGTTCCCAGCTTTACATTGAAGGCCTTTGTCATGGAAATTTGTTAGAAGAAGAGGTAATCAGCCTTTCCAATATATTTAAAACAAATTTTCCAGTAGAACCACTTCCTATCGAATTGAGGCATAAGGATCACTGTATATGCCTTCCTCCTTATGCTAACCTCATTAGAGAAGCCAGTGTGAAGAATAATTCTGAAACAAACTCTGTATTTGAGCATGGACTGGATGATGATTCCTTTCAGAATTATAAAGATGGACTAATGGCAAATCTATTGGAGAGAGATGAAATCCTCGCACGTGAAACATCTAGGCTTTGGGAGGAGATTACCAATAAAAGGCATACGTTTGACTGGCCAAAAAAGGTAGCTGAAGAGGTCAGAAGCCTTCAGAAGGGGGACGTTATTAACTTTTACAAAACGTATTTGCAACCATCATCTCCCAAGTGTCGGAGACTTGCAATTCGTGTTTGGGGTTGCAACACTGATTTCAAAGAAGCTGAAGCACCACCAGAGTCTATACAAGTCATTCAGGACCTTGCAGCCTTTAAGATGTCATCTGAGTTCTATCCTCATGGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

912

Amino Acids

105.01

Weight (kDa)

5.39

Isoelectric Point (pI)

43.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 72 - 153 4.8e-23 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 220 - 395 2.2e-18 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 402 - 703 5.1e-81 Middle or third domain of peptidase_M16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2406
AatII GACGTC 1 cut(s) 2065
AccB1I GGYRCC 1 cut(s) 449
AccI GTMKAC 1 cut(s) 1657
AciI CCGC 4 cut(s) 547, 1036, 1157, 1735
AclWI GGATC 9 cut(s) 97, 107, 149, 1227, 1529, 1542, 2027, 2040, 2295
AcoI YGGCCR 1 cut(s) 2510
AcsI RAATTY 6 cut(s) 599, 1258, 1938, 1957, 2197, 2245
AcuI CTGAAG 8 cut(s) 162, 1336, 1515, 1766, 1913, 2528, 2547, 2676
AcvI CACGTG 1 cut(s) 2456
AcyI GRCGYC 1 cut(s) 2062
AfaI GTAC 8 cut(s) 325, 416, 463, 680, 1307, 1688, 1702, 2075
AfiI CCNNNNNNNGG 6 cut(s) 274, 424, 1478, 2493, 2548, 2604
AflII CTTAAG 1 cut(s) 443
AflIII ACRYGT 2 cut(s) 1282, 1436
AjnI CCWGG 6 cut(s) 580, 801, 1173, 1182, 1227, 1588
Alw26I GTCTC 5 cut(s) 48, 678, 1304, 2053, 2602
AlwI GGATC 9 cut(s) 97, 107, 149, 1227, 1529, 1542, 2027, 2040, 2295
Ama87I CYCGRG 1 cut(s) 792
AoxI GGCC 5 cut(s) 88, 565, 1836, 2182, 2510
ApeKI GCWGC 6 cut(s) 9, 227, 230, 233, 255, 2696
ApoI RAATTY 6 cut(s) 599, 1258, 1938, 1957, 2197, 2245
ArsI GACNNNNNNTTYG 2 cut(s) 2601, 2633
Asp700I GAANNNNTTC 3 cut(s) 558, 1580, 2262
AspS9I GGNCC 3 cut(s) 788, 821, 2689
AsuC2I CCSGG 1 cut(s) 524
AsuHPI GGTGA 7 cut(s) 191, 439, 578, 1042, 1146, 1604, 1687
AvaI CYCGRG 1 cut(s) 792
AvaII GGWCC 3 cut(s) 788, 821, 2689
AxyI CCTNAGG 1 cut(s) 273
BalI TGGCCA 1 cut(s) 2512
BamHI GGATCC 2 cut(s) 1534, 2032
BanI GGYRCC 1 cut(s) 449
BarI GAAGNNNNNNTAC 4 cut(s) 1670, 1702, 2247, 2279
BauI CACGAG 1 cut(s) 396
BbrPI CACGTG 1 cut(s) 2456
BbvI GCAGC 6 cut(s) 21, 239, 242, 245, 267, 2708
BccI CCATC 2 cut(s) 2405, 2596
BciT130I CCWGG 6 cut(s) 582, 803, 1175, 1184, 1229, 1590
BclI TGATCA 2 cut(s) 36, 1065
BcnI CCSGG 1 cut(s) 524
BcoDI GTCTC 5 cut(s) 48, 678, 1304, 2053, 2602
BfaI CTAG 7 cut(s) 299, 713, 854, 999, 1853, 1953, 2466
BfrI CTTAAG 1 cut(s) 443
BglII AGATCT 1 cut(s) 1680
BisI GCNGC 7 cut(s) 10, 228, 231, 234, 256, 547, 2697
BlsI GCNGC 7 cut(s) 11, 229, 232, 235, 257, 548, 2698
BmcAI AGTACT 2 cut(s) 463, 1702
Bme1390I CCNGG 7 cut(s) 524, 582, 803, 1175, 1184, 1229, 1590
Bme18I GGWCC 3 cut(s) 788, 821, 2689
BmeT110I CYCGRG 1 cut(s) 792
BmgT120I GGNCC 3 cut(s) 788, 821, 2689
BmiI GGNNCC 5 cut(s) 451, 790, 822, 1536, 2034
BmrFI CCNGG 7 cut(s) 524, 582, 803, 1175, 1184, 1229, 1590
BmsI GCATC 2 cut(s) 622, 1570
BpmI CTGGAG 3 cut(s) 824, 1166, 1524
Bpu10I CCTNAGC 1 cut(s) 2154
BpuEI CTTGAG 5 cut(s) 101, 767, 977, 1826, 2077
BpuMI CCSGG 1 cut(s) 524
BsaAI YACGTR 1 cut(s) 2456
BsaHI GRCGYC 1 cut(s) 2062
BsaJI CCNNGG 4 cut(s) 694, 1227, 1472, 1715
BsaXI ACNNNNNCTCC 4 cut(s) 936, 966, 1997, 2027
Bsc4I CCNNNNNNNGG 6 cut(s) 274, 424, 1478, 2493, 2548, 2604
Bse1I ACTGG 5 cut(s) 1462, 2252, 2334, 2385, 2513
Bse21I CCTNAGG 1 cut(s) 273
Bse3DI GCAATG 4 cut(s) 158, 243, 514, 1556
BseBI CCWGG 6 cut(s) 582, 803, 1175, 1184, 1229, 1590
BseDI CCNNGG 4 cut(s) 694, 1227, 1472, 1715
BseGI GGATG 5 cut(s) 634, 1237, 1324, 1816, 2389
BseLI CCNNNNNNNGG 6 cut(s) 274, 424, 1478, 2493, 2548, 2604
BseMI GCAATG 4 cut(s) 158, 243, 514, 1556
BseMII CTCAG 8 cut(s) 108, 161, 199, 264, 1033, 2030, 2145, 2706
BseNI ACTGG 5 cut(s) 1462, 2252, 2334, 2385, 2513
BseRI GAGGAG 1 cut(s) 2492
BseXI GCAGC 6 cut(s) 21, 239, 242, 245, 267, 2708
BseYI CCCAGC 2 cut(s) 1847, 2166
BshFI GGCC 5 cut(s) 90, 567, 1838, 2184, 2512
BshNI GGYRCC 1 cut(s) 449
BsiHKCI CYCGRG 1 cut(s) 792
BsiSI CCGG 1 cut(s) 523
BslFI GGGAC 3 cut(s) 774, 1310, 2567
BslI CCNNNNNNNGG 6 cut(s) 274, 424, 1478, 2493, 2548, 2604
BsmAI GTCTC 5 cut(s) 48, 678, 1304, 2053, 2602
BsmBI CGTCTC 1 cut(s) 2053
BsmFI GGGAC 3 cut(s) 774, 1310, 2567
BsmI GAATGC 1 cut(s) 838
BsnI GGCC 5 cut(s) 90, 567, 1838, 2184, 2512
BsoBI CYCGRG 1 cut(s) 792
Bsp1407I TGTACA 1 cut(s) 678
Bsp19I CCATGG 1 cut(s) 694
BspACI CCGC 4 cut(s) 547, 1036, 1157, 1735
BspANI GGCC 5 cut(s) 90, 567, 1838, 2184, 2512
BspCNI CTCAG 8 cut(s) 109, 160, 198, 265, 1032, 2031, 2146, 2707
BspLI GGNNCC 5 cut(s) 451, 790, 822, 1536, 2034
BspPI GGATC 9 cut(s) 97, 107, 149, 1227, 1529, 1542, 2027, 2040, 2295
BspQI GCTCTTC 1 cut(s) 317
BspT107I GGYRCC 1 cut(s) 449
BspTI CTTAAG 1 cut(s) 443
BsrDI GCAATG 4 cut(s) 158, 243, 514, 1556
BsrGI TGTACA 1 cut(s) 678
BsrI ACTGG 5 cut(s) 1462, 2252, 2334, 2385, 2513
BssECI CCNNGG 4 cut(s) 694, 1227, 1472, 1715
BssNAI GTATAC 1 cut(s) 1658
BssNI GRCGYC 1 cut(s) 2062
BssSI CACGAG 1 cut(s) 396
BssT1I CCWWGG 2 cut(s) 694, 1472
Bst1107I GTATAC 1 cut(s) 1658
Bst2BI CACGAG 1 cut(s) 396
Bst2UI CCWGG 6 cut(s) 582, 803, 1175, 1184, 1229, 1590
Bst4CI ACNGT 8 cut(s) 356, 1419, 1700, 1791, 1948, 2078, 2120, 2294
Bst6I CTCTTC 6 cut(s) 317, 396, 2053, 2094, 2205, 2523
BstACI GRCGYC 1 cut(s) 2062
BstAFI CTTAAG 1 cut(s) 443
BstAUI TGTACA 1 cut(s) 678
BstBAI YACGTR 1 cut(s) 2456
BstC8I GCNNGC 3 cut(s) 288, 391, 1469
BstDSI CCRYGG 2 cut(s) 694, 1715
BstEII GGTNACC 1 cut(s) 584
BstENI CCTNNNNNAGG 1 cut(s) 2546
BstF5I GGATG 5 cut(s) 634, 1237, 1324, 1816, 2389
BstMAI GTCTC 5 cut(s) 48, 678, 1304, 2053, 2602
BstMWI GCNNNNNNNGC 2 cut(s) 233, 1844
BstNI CCWGG 6 cut(s) 582, 803, 1175, 1184, 1229, 1590
BstNSI RCATGY 5 cut(s) 310, 393, 880, 1286, 1440
BstPI GGTNACC 1 cut(s) 584
BstSCI CCNGG 7 cut(s) 522, 580, 801, 1173, 1182, 1227, 1588
BstV1I GCAGC 6 cut(s) 21, 239, 242, 245, 267, 2708
BstX2I RGATCY 3 cut(s) 1534, 1680, 2032
BstXI CCANNNNNNTGG 1 cut(s) 1190
BstYI RGATCY 3 cut(s) 1534, 1680, 2032
BstZ17I GTATAC 1 cut(s) 1658
Bsu36I CCTNAGG 1 cut(s) 273
BsuRI GGCC 5 cut(s) 90, 567, 1838, 2184, 2512
BtgI CCRYGG 2 cut(s) 694, 1715
BtsCI GGATG 5 cut(s) 634, 1237, 1324, 1816, 2389
BtsI GCAGTG 2 cut(s) 93, 758
BtsIMutI CAGTG 9 cut(s) 93, 138, 758, 1027, 1424, 1469, 2290, 2341, 2637
Cac8I GCNNGC 3 cut(s) 288, 391, 1469
Cfr13I GGNCC 3 cut(s) 788, 821, 2689
CseI GACGC 1 cut(s) 647
CsiI ACCWGGT 1 cut(s) 580
Csp6I GTAC 8 cut(s) 324, 415, 462, 679, 1306, 1687, 1701, 2074
CviQI GTAC 8 cut(s) 324, 415, 462, 679, 1306, 1687, 1701, 2074
DraI TTTAAA 2 cut(s) 1708, 2239
EaeI YGGCCR 1 cut(s) 2510
Eam1104I CTCTTC 6 cut(s) 317, 396, 2053, 2094, 2205, 2523
EarI CTCTTC 6 cut(s) 317, 396, 2053, 2094, 2205, 2523
Eco130I CCWWGG 2 cut(s) 694, 1472
Eco147I AGGCCT 1 cut(s) 2184
Eco32I GATATC 2 cut(s) 778, 1383
Eco47I GGWCC 3 cut(s) 788, 821, 2689
Eco57I CTGAAG 8 cut(s) 162, 1336, 1515, 1766, 1913, 2528, 2547, 2676
Eco72I CACGTG 1 cut(s) 2456
Eco81I CCTNAGG 1 cut(s) 273
Eco88I CYCGRG 1 cut(s) 792
Eco91I GGTNACC 1 cut(s) 584
EcoNI CCTNNNNNAGG 1 cut(s) 2546
EcoO109I RGGNCCY 3 cut(s) 788, 821, 2689
EcoO65I GGTNACC 1 cut(s) 584
EcoRII CCWGG 6 cut(s) 580, 801, 1173, 1182, 1227, 1588
EcoRV GATATC 2 cut(s) 778, 1383
EcoT14I CCWWGG 2 cut(s) 694, 1472
EcoT22I ATGCAT 2 cut(s) 391, 1729
ErhI CCWWGG 2 cut(s) 694, 1472
Esp3I CGTCTC 1 cut(s) 2053
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 608, 640
FaqI GGGAC 3 cut(s) 774, 1310, 2567
FauNDI CATATG 1 cut(s) 1972
FbaI TGATCA 2 cut(s) 36, 1065
FblI GTMKAC 1 cut(s) 1657
Fnu4HI GCNGC 7 cut(s) 10, 228, 231, 234, 256, 547, 2697
FokI GGATG 5 cut(s) 641, 1244, 1331, 1823, 2396
Fsp4HI GCNGC 7 cut(s) 10, 228, 231, 234, 256, 547, 2697
FspBI CTAG 7 cut(s) 299, 713, 854, 999, 1853, 1953, 2466
GluI GCNGC 7 cut(s) 10, 228, 231, 234, 256, 547, 2697
GsaI CCCAGC 2 cut(s) 1851, 2170
GsuI CTGGAG 3 cut(s) 824, 1166, 1524
HaeIII GGCC 5 cut(s) 90, 567, 1838, 2184, 2512
HapII CCGG 1 cut(s) 523
HgaI GACGC 1 cut(s) 647
Hin1I GRCGYC 1 cut(s) 2062
HincII GTYRAC 1 cut(s) 662
HindII GTYRAC 1 cut(s) 662
HindIII AAGCTT 3 cut(s) 622, 1691, 1916
HinfI GANTC 5 cut(s) 731, 1082, 2041, 2390, 2669
HpaII CCGG 1 cut(s) 523
HphI GGTGA 7 cut(s) 191, 439, 578, 1042, 1146, 1604, 1687
Hpy166II GTNNAC 7 cut(s) 182, 662, 799, 874, 1033, 1306, 1658
Hpy8I GTNNAC 7 cut(s) 182, 662, 799, 874, 1033, 1306, 1658
HpyCH4III ACNGT 8 cut(s) 356, 1419, 1700, 1791, 1948, 2078, 2120, 2294
HpyCH4IV ACGT 7 cut(s) 946, 1267, 2062, 2455, 2501, 2556, 2576
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 1844
HpySE526I ACGT 7 cut(s) 946, 1267, 2062, 2455, 2501, 2556, 2576
Hsp92I GRCGYC 1 cut(s) 2062
Ksp22I TGATCA 2 cut(s) 36, 1065
LguI GCTCTTC 1 cut(s) 317
LmnI GCTCC 5 cut(s) 189, 885, 1093, 1888, 2005
Lsp1109I GCAGC 6 cut(s) 21, 239, 242, 245, 267, 2708
LweI GCATC 2 cut(s) 622, 1570
MabI ACCWGGT 1 cut(s) 580
MaeI CTAG 7 cut(s) 299, 713, 854, 999, 1853, 1953, 2466
MaeII ACGT 7 cut(s) 946, 1267, 2062, 2455, 2501, 2556, 2576
MaeIII GTNAC 4 cut(s) 356, 584, 1482, 2042
MfeI CAATTG 1 cut(s) 1446
MflI RGATCY 3 cut(s) 1534, 1680, 2032
MlsI TGGCCA 1 cut(s) 2512
MluNI TGGCCA 1 cut(s) 2512
MlyI GAGTC 3 cut(s) 1076, 2050, 2678
MmeI TCCRAC 3 cut(s) 226, 1535, 2585
Mox20I TGGCCA 1 cut(s) 2512
Mph1103I ATGCAT 2 cut(s) 391, 1729
MroXI GAANNNNTTC 3 cut(s) 558, 1580, 2262
MscI TGGCCA 1 cut(s) 2512
MslI CAYNNNNRTG 4 cut(s) 901, 1435, 1866, 2027
Msp20I TGGCCA 1 cut(s) 2512
MspCI CTTAAG 1 cut(s) 443
MspI CCGG 1 cut(s) 523
MspR9I CCNGG 7 cut(s) 524, 582, 803, 1175, 1184, 1229, 1590
MunI CAATTG 1 cut(s) 1446
Mva1269I GAATGC 1 cut(s) 838
MvaI CCWGG 6 cut(s) 582, 803, 1175, 1184, 1229, 1590
MwoI GCNNNNNNNGC 2 cut(s) 233, 1844
NciI CCSGG 1 cut(s) 524
NcoI CCATGG 1 cut(s) 694
NdeI CATATG 1 cut(s) 1972
NlaIV GGNNCC 5 cut(s) 451, 790, 822, 1536, 2034
NmuCI GTSAC 3 cut(s) 584, 1482, 2042
NsiI ATGCAT 2 cut(s) 391, 1729
NspI RCATGY 5 cut(s) 310, 393, 880, 1286, 1440
PaeI GCATGC 1 cut(s) 393
PceI AGGCCT 1 cut(s) 2184
PciI ACATGT 2 cut(s) 1282, 1436
PciSI GCTCTTC 1 cut(s) 317
PctI GAATGC 1 cut(s) 838
PdmI GAANNNNTTC 3 cut(s) 558, 1580, 2262
PfeI GAWTC 2 cut(s) 731, 2390
PfoI TCCNGGA 2 cut(s) 1173, 1182
PkrI GCNGC 7 cut(s) 11, 229, 232, 235, 257, 548, 2698
PleI GAGTC 3 cut(s) 1076, 2049, 2677
PmaCI CACGTG 1 cut(s) 2456
PmlI CACGTG 1 cut(s) 2456
PpsI GAGTC 3 cut(s) 1076, 2049, 2677
Ppu21I YACGTR 1 cut(s) 2456
PpuMI RGGWCCY 3 cut(s) 788, 821, 2689
PscI ACATGT 2 cut(s) 1282, 1436
PsiI TTATAA 1 cut(s) 2406
Psp5II RGGWCCY 3 cut(s) 788, 821, 2689
Psp6I CCWGG 6 cut(s) 580, 801, 1173, 1182, 1227, 1588
PspCI CACGTG 1 cut(s) 2456
PspEI GGTNACC 1 cut(s) 584
PspFI CCCAGC 2 cut(s) 1847, 2166
PspGI CCWGG 6 cut(s) 580, 801, 1173, 1182, 1227, 1588
PspN4I GGNNCC 5 cut(s) 451, 790, 822, 1536, 2034
PspPI GGNCC 3 cut(s) 788, 821, 2689
PspPPI RGGWCCY 3 cut(s) 788, 821, 2689
PsuI RGATCY 3 cut(s) 1534, 1680, 2032
RsaI GTAC 8 cut(s) 325, 416, 463, 680, 1307, 1688, 1702, 2075
RsaNI GTAC 8 cut(s) 324, 415, 462, 679, 1306, 1687, 1701, 2074
RseI CAYNNNNRTG 4 cut(s) 901, 1435, 1866, 2027
SapI GCTCTTC 1 cut(s) 317
SatI GCNGC 7 cut(s) 10, 228, 231, 234, 256, 547, 2697
Sau96I GGNCC 3 cut(s) 788, 821, 2689
ScaI AGTACT 2 cut(s) 463, 1702
SchI GAGTC 3 cut(s) 1076, 2050, 2678
ScrFI CCNGG 7 cut(s) 524, 582, 803, 1175, 1184, 1229, 1590
SexAI ACCWGGT 1 cut(s) 580
SfaNI GCATC 2 cut(s) 622, 1570
SinI GGWCC 3 cut(s) 788, 821, 2689
SmiMI CAYNNNNRTG 4 cut(s) 901, 1435, 1866, 2027
SmlI CTYRAG 6 cut(s) 80, 443, 746, 956, 1805, 2056
SmoI CTYRAG 6 cut(s) 80, 443, 746, 956, 1805, 2056
SphI GCATGC 1 cut(s) 393
SseBI AGGCCT 1 cut(s) 2184
SsiI CCGC 4 cut(s) 547, 1036, 1157, 1735
SspMI CTAG 7 cut(s) 299, 713, 854, 999, 1853, 1953, 2466
StuI AGGCCT 1 cut(s) 2184
StyD4I CCNGG 7 cut(s) 522, 580, 801, 1173, 1182, 1227, 1588
StyI CCWWGG 2 cut(s) 694, 1472
TaaI ACNGT 8 cut(s) 356, 1419, 1700, 1791, 1948, 2078, 2120, 2294
TaiI ACGT 7 cut(s) 949, 1270, 2065, 2458, 2504, 2559, 2579
TaqI TCGA 2 cut(s) 1797, 2271
TatI WGTACW 4 cut(s) 323, 461, 678, 1700
TauI GCSGC 1 cut(s) 549
TfiI GAWTC 2 cut(s) 731, 2390
TscAI CASTG 9 cut(s) 100, 145, 758, 1027, 1424, 1469, 2297, 2341, 2644
TseFI GTSAC 3 cut(s) 584, 1482, 2042
TseI GCWGC 6 cut(s) 9, 227, 230, 233, 255, 2696
Tsp45I GTSAC 3 cut(s) 584, 1482, 2042
TspGWI ACGGA 1 cut(s) 387
TspRI CASTG 9 cut(s) 100, 145, 758, 1027, 1424, 1469, 2297, 2341, 2644
Vha464I CTTAAG 1 cut(s) 443
VpaK11BI GGWCC 3 cut(s) 788, 821, 2689
XagI CCTNNNNNAGG 1 cut(s) 2546
XapI RAATTY 6 cut(s) 599, 1258, 1938, 1957, 2197, 2245
XbaI TCTAGA 1 cut(s) 298
XceI RCATGY 5 cut(s) 310, 393, 880, 1286, 1440
XmiI GTMKAC 1 cut(s) 1657
XmnI GAANNNNTTC 3 cut(s) 558, 1580, 2262
XspI CTAG 7 cut(s) 299, 713, 854, 999, 1853, 1953, 2466
ZraI GACGTC 1 cut(s) 2063
ZrmI AGTACT 2 cut(s) 463, 1702
Zsp2I ATGCAT 2 cut(s) 391, 1729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.