Rroxscaffold_1G00008310

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000183
Physical Location & Seq
Forward (+)
10502499 .. 10510810
8312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00008310.1

Sequence Viewer

Length: 2421 bp
ATGGGTCTGATGGGTCGCTGCACTTTCTCCTCGGACGATATTGTAATAAAGTCTCCGAATGATAAGAAATTGTACAGACTAATTAAGCTTGAGAATGGCCTCACTGCATTGCTCATTCATGACCCTGAGACATACCCACAAGGGTTTGGCCTTGAACCCAGTGAAGAAGATGAAGCAGCAGCTAAAGGTGGAGATGGTGCCTCAGAGACTAAGAAGGCAGCAGCGGCAATGTGCGTTGGAATGGGCAGCTTCTCTGACCCTATGGAAGCACAGGGGCTTGCGCACTTTCTTGAACATATGCTCTTCATGGGGAGTACAAAGTTTCCAGTTGAAAATGAGTATAACAGTTACTTGTCCAAGCATGGGGGCTCATCAAATGCTTACACAAAGGAAGAGCACACTTGCTACCATTTTGACGTGAAACGAGAGTTTCTTAAAGGTGCCTTGAAAAGGGTTCTGCATAGCGATTTGTGGCGCCTTCAACAACTTCGAGACCATACATCCTCACCTGGAAATAAGAAGAGCTTGGATGGTGCAAAGGAAAAAGGGATAAACTTGCAGGAACAGATATTGAAATTGTACAGAGACTATTACCATGGTGGATTAATGAAGCTAGTTGTTATTGGTGGAGAATCTCTCAATGTACTTGAGCAGTGGGTTCTGAAGTTGTTTGCAGACATCAGAAAAGGTCCCCAAGTAAATCTTGAGTTCAAGGCAGAAGGTCCTATTTGGAACGTTGGAAAGCTTTACAGGCTAGAGGCTGTTAATGATGTTCCTATGCTCAACTTGGCATGGACGCTTCCATGTCTTACTCAACACTATTTGAAGAGCCCAGAATATTATTTATGGCATCTCCTTGCGCATGAGAGCAGGGGAGGTTTGCAGTTCTACCTCAAAACTAGAGGGTGGGCAGTATCTCTAAGTGCTTGGGTGGAGAGCTATTCTGTGGCTTATGTCTTTGGCATGAATATTTACCTCACCGACTCTGGATTGGAGAAGATTTTTGAAATTATTGGATTTGTAGAGGAGCAGCCCCAGGACGACTATGCTTCAGAACTTGCAGGAAATTTACTACTTTATCCAGCAGAGCATGTTATTTCTGCGCAATGTGGGTATGAGATTTGGGATGAGGAATTGATTGTATATGTTCTTAGTTTCTTCAGACCAGACAACATGAGGATGGATGTGATATCAAAGTTTTCAACTATGTCCAAAGATTTTCAGTGCGAGCCTTGGTTTGGGTCACATTATATTGAGGAAGATGTATCTCCGTTTTTGATGGATTTGTGGAAGGATCCTCCAGAGATTGATGTTTCGTTGCATCTCCGAGAAAAGAATGAGTTTATTCCTTCTGATTTTTCCATTCGTTCTGATGGTCTTGATACTACATATACATCTTCTCCGAGATGTATACTTGATGAACCTTTGCTTAAGTTCTGGTACAAGCTTGATCGTACTTTTAAAGTTCCACGGGCAAATATATACTTTCGAATCAGTCTAAAGGGTGGATTTGATAACTTGAAGAGTTCTCTGTTGACTGTACTATATATCGACCTTCTTAGAGATGAGCTGAATGAGATTTTATATCAGCTGTGGGGCTTCAATGATAAGCTTCCAGCTGTATTGTCAACAATTCTGACAACAACCAAAAATTTCCTGCCAACTTATGATCGTTTCAAGGTTATTAAAGAAAATATGGAGCGTCTGATAAAGAACACTAATATGAATCCTCTGAGTCATGCTGAATACTTGAAACTGCAAGTTTTGTACCAGAGATTCTACGATGTAGATGACCAGTTGCATGTTTTAAATGGATTGTCTGTTTCTGATGTGAAGTCATTTATTCCAGAGCTTTGGTCCCAGCTTTACATTGAAGGCCTTGGTCATGGAAATCTGTTAGAAGAAGAAGCAATTACCCTTTTGAATATGGTGAAAACAATTTTTTCTGGACAACCACTTCCTACCGAACTGATGCTTAAGAACAAATGTATTTGTCTCGCTCCTGATGCTAACCTCATTAGAGATGTCAGTGTGAAGTATAAGACTGAAACAAACTCTGTGATTCAGCTATATTTTCAAATTGAGCGGGCAGTGGGGATTGACTCCACCAGATTGAGAGCATTAATAGATCTTTTTGATGAAATTATAGACGAACCACTTTACGATCAACTAAGGTATACATTTGACTATGCAAAGAGGGTGGCAGAAGAGCTCAGCAGTCTACAGAAGGAGGATGTTGTCAACTTTTACAAGACCTACTTGCAACAATCATCTCCCAAGCGTCGGAGACTTGGCATTCGTGTTTGGGGTTGCAACACTGACTTGAAAGAAGCTGAAGAATCGCGACCGGAGTCTGTGCAGGTCATTGAAGACCTTGAAGCCTTTAAGATGTCATCTGAGTTCTATCCTCACGGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

806

Amino Acids

92.72

Weight (kDa)

5.25

Isoelectric Point (pI)

44.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 69 - 151 1.8e-24 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 188 - 336 2.1e-16 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 340 - 530 2.1e-49 Middle or third domain of peptidase_M16
Peptidase_M16_M PF16187 531 - 601 2.4e-11 Middle or third domain of peptidase_M16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 3 cut(s) 282, 861, 1104
Acc36I ACCTGC 1 cut(s) 2350
AccB1I GGYRCC 3 cut(s) 197, 440, 474
AccBSI CCGCTC 1 cut(s) 2086
AccI GTMKAC 3 cut(s) 1411, 2177, 2221
AccII CGCG 1 cut(s) 2344
AciI CCGC 2 cut(s) 224, 2086
AclI AACGTT 1 cut(s) 735
AclWI GGATC 2 cut(s) 1289, 1302
AcsI RAATTY 2 cut(s) 1066, 1651
AcuI CTGAAG 4 cut(s) 683, 1035, 1144, 2355
AcyI GRCGYC 1 cut(s) 475
AfaI GTAC 8 cut(s) 74, 316, 581, 645, 1442, 1456, 1542, 1769
AfiI CCNNNNNNNGG 4 cut(s) 363, 450, 1238, 2283
AflII CTTAAG 2 cut(s) 1430, 1976
AjiI CACGTC 1 cut(s) 418
AjnI CCWGG 2 cut(s) 508, 1035
Alw21I GWGCWC 2 cut(s) 399, 2214
Alw26I GTCTC 7 cut(s) 57, 122, 200, 486, 579, 2000, 2281
AlwI GGATC 2 cut(s) 1289, 1302
AoxI GGCC 3 cut(s) 97, 148, 1876
ApeKI GCWGC 7 cut(s) 18, 176, 179, 218, 221, 246, 1030
ApoI RAATTY 2 cut(s) 1066, 1651
ArsI GACNNNNNNTTYG 2 cut(s) 2280, 2312
AseI ATTAAT 2 cut(s) 605, 2123
AspLEI GCGC 4 cut(s) 283, 477, 862, 1105
AspS9I GGNCC 3 cut(s) 689, 722, 1857
AsuHPI GGTGA 3 cut(s) 498, 970, 1942
AsuII TTCGAA 1 cut(s) 1489
AvaII GGWCC 3 cut(s) 689, 722, 1857
BamHI GGATCC 1 cut(s) 1294
BanI GGYRCC 3 cut(s) 197, 440, 474
BanII GRGCYC 3 cut(s) 371, 833, 2214
BbsI GAAGAC 1 cut(s) 2376
Bbv12I GWGCWC 2 cut(s) 399, 2214
BbvI GCAGC 7 cut(s) 5, 188, 191, 230, 233, 258, 1042
BccI CCATC 6 cut(s) 4, 188, 524, 1174, 1273, 1367
BciT130I CCWGG 2 cut(s) 510, 1037
BcoDI GTCTC 7 cut(s) 57, 122, 200, 486, 579, 2000, 2281
BfaI CTAG 3 cut(s) 614, 755, 900
BfmI CTRYAG 1 cut(s) 2222
BfoI RGCGCY 1 cut(s) 478
BfrI CTTAAG 2 cut(s) 1430, 1976
BfuAI ACCTGC 1 cut(s) 2350
BglII AGATCT 1 cut(s) 2128
BisI GCNGC 8 cut(s) 19, 177, 180, 219, 222, 225, 247, 1031
BlpI GCTNAGC 1 cut(s) 2213
BlsI GCNGC 8 cut(s) 20, 178, 181, 220, 223, 226, 248, 1032
Bme1390I CCNGG 2 cut(s) 510, 1037
Bme18I GGWCC 3 cut(s) 689, 722, 1857
BmgBI CACGTC 1 cut(s) 418
BmgT120I GGNCC 3 cut(s) 689, 722, 1857
BmiI GGNNCC 6 cut(s) 199, 442, 476, 691, 1296, 1859
BmrFI CCNGG 2 cut(s) 510, 1037
BmrI ACTGGG 1 cut(s) 153
BmsI GCATC 4 cut(s) 859, 1330, 1962, 1996
BmuI ACTGGG 1 cut(s) 153
BoxI GACNNNNGTC 1 cut(s) 2350
BpiI GAAGAC 1 cut(s) 2376
BplI GAGNNNNNCTC 2 cut(s) 621, 653
BpmI CTGGAG 1 cut(s) 1284
Bpu1102I GCTNAGC 1 cut(s) 2213
Bpu14I TTCGAA 1 cut(s) 1489
BpuEI CTTGAG 3 cut(s) 110, 668, 725
BsaHI GRCGYC 1 cut(s) 475
BsaI GGTCTC 1 cut(s) 486
BsaJI CCNNGG 6 cut(s) 30, 595, 1035, 1232, 1469, 1879
BsaWI WCCGGW 1 cut(s) 2347
BsaXI ACNNNNNCTCC 2 cut(s) 1384, 1414
Bsc4I CCNNNNNNNGG 4 cut(s) 363, 450, 1238, 2283
Bse1I ACTGG 3 cut(s) 159, 326, 1795
Bse3DI GCAATG 3 cut(s) 107, 234, 1112
BseBI CCWGG 2 cut(s) 510, 1037
BseDI CCNNGG 6 cut(s) 30, 595, 1035, 1232, 1469, 1879
BseGI GGATG 6 cut(s) 500, 535, 1132, 1185, 1189, 2239
BseLI CCNNNNNNNGG 4 cut(s) 363, 450, 1238, 2283
BseMI GCAATG 3 cut(s) 107, 234, 1112
BseMII CTCAG 5 cut(s) 117, 216, 1724, 2227, 2388
BseNI ACTGG 3 cut(s) 159, 326, 1795
BseRI GAGGAG 2 cut(s) 19, 1040
BseXI GCAGC 7 cut(s) 5, 188, 191, 230, 233, 258, 1042
BseYI CCCAGC 1 cut(s) 1860
BsgI GTGCAG 2 cut(s) 4, 2378
Bsh1236I CGCG 1 cut(s) 2344
Bsh1285I CGRYCG 1 cut(s) 2348
BshFI GGCC 3 cut(s) 99, 150, 1878
BshNI GGYRCC 3 cut(s) 197, 440, 474
BsiEI CGRYCG 1 cut(s) 2348
BsiHKAI GWGCWC 2 cut(s) 399, 2214
BsiSI CCGG 1 cut(s) 2348
BslFI GGGAC 2 cut(s) 675, 1843
BslI CCNNNNNNNGG 4 cut(s) 363, 450, 1238, 2283
BsmAI GTCTC 7 cut(s) 57, 122, 200, 486, 579, 2000, 2281
BsmFI GGGAC 2 cut(s) 675, 1843
BsmI GAATGC 1 cut(s) 2295
BsnI GGCC 3 cut(s) 99, 150, 1878
Bso31I GGTCTC 1 cut(s) 486
Bsp119I TTCGAA 1 cut(s) 1489
Bsp1286I GDGCHC 4 cut(s) 371, 399, 833, 2214
Bsp1407I TGTACA 2 cut(s) 72, 579
Bsp143I GATC 5 cut(s) 1294, 1450, 1669, 2128, 2164
Bsp1720I GCTNAGC 1 cut(s) 2213
Bsp19I CCATGG 1 cut(s) 595
Bsp68I TCGCGA 1 cut(s) 2344
BspACI CCGC 2 cut(s) 224, 2086
BspANI GGCC 3 cut(s) 99, 150, 1878
BspCNI CTCAG 5 cut(s) 118, 215, 1725, 2226, 2389
BspFNI CGCG 1 cut(s) 2344
BspHI TCATGA 1 cut(s) 118
BspLI GGNNCC 6 cut(s) 199, 442, 476, 691, 1296, 1859
BspMI ACCTGC 1 cut(s) 2350
BspPI GGATC 2 cut(s) 1289, 1302
BspQI GCTCTTC 5 cut(s) 308, 387, 515, 821, 2202
BspT104I TTCGAA 1 cut(s) 1489
BspT107I GGYRCC 3 cut(s) 197, 440, 474
BspTI CTTAAG 2 cut(s) 1430, 1976
BspTNI GGTCTC 1 cut(s) 486
BsrBI CCGCTC 1 cut(s) 2086
BsrDI GCAATG 3 cut(s) 107, 234, 1112
BsrGI TGTACA 2 cut(s) 72, 579
BsrI ACTGG 3 cut(s) 159, 326, 1795
BssECI CCNNGG 6 cut(s) 30, 595, 1035, 1232, 1469, 1879
BssMI GATC 5 cut(s) 1294, 1450, 1669, 2128, 2164
BssNAI GTATAC 2 cut(s) 1412, 2178
BssNI GRCGYC 1 cut(s) 475
BssT1I CCWWGG 3 cut(s) 595, 1232, 1879
Bst1107I GTATAC 2 cut(s) 1412, 2178
Bst2UI CCWGG 2 cut(s) 510, 1037
Bst4CI ACNGT 3 cut(s) 347, 1540, 2414
Bst6I CTCTTC 6 cut(s) 308, 387, 515, 821, 1517, 2202
BstACI GRCGYC 1 cut(s) 475
BstAFI CTTAAG 2 cut(s) 1430, 1976
BstAUI TGTACA 2 cut(s) 72, 579
BstBI TTCGAA 1 cut(s) 1489
BstC8I GCNNGC 3 cut(s) 279, 1229, 2088
BstDSI CCRYGG 2 cut(s) 595, 1469
BstENI CCTNNNNNAGG 1 cut(s) 448
BstF5I GGATG 6 cut(s) 500, 535, 1132, 1185, 1189, 2239
BstFNI CGCG 1 cut(s) 2344
BstH2I RGCGCY 1 cut(s) 478
BstHHI GCGC 4 cut(s) 283, 477, 862, 1105
BstKTI GATC 5 cut(s) 1297, 1453, 1672, 2131, 2167
BstMAI GTCTC 7 cut(s) 57, 122, 200, 486, 579, 2000, 2281
BstMBI GATC 5 cut(s) 1294, 1450, 1669, 2128, 2164
BstMCI CGRYCG 1 cut(s) 2348
BstMWI GCNNNNNNNGC 3 cut(s) 224, 751, 2006
BstNI CCWGG 2 cut(s) 510, 1037
BstNSI RCATGY 2 cut(s) 1094, 1805
BstPAI GACNNNNGTC 1 cut(s) 2350
BstSCI CCNGG 2 cut(s) 508, 1035
BstSFI CTRYAG 1 cut(s) 2222
BstUI CGCG 1 cut(s) 2344
BstV1I GCAGC 7 cut(s) 5, 188, 191, 230, 233, 258, 1042
BstV2I GAAGAC 1 cut(s) 2376
BstX2I RGATCY 2 cut(s) 1294, 2128
BstXI CCANNNNNNTGG 1 cut(s) 1854
BstYI RGATCY 2 cut(s) 1294, 2128
BstZ17I GTATAC 2 cut(s) 1412, 2178
BsuRI GGCC 3 cut(s) 99, 150, 1878
BtgI CCRYGG 2 cut(s) 595, 1469
BtrI CACGTC 1 cut(s) 418
BtsCI GGATG 6 cut(s) 500, 535, 1132, 1185, 1189, 2239
BtsI GCAGTG 3 cut(s) 102, 659, 2097
BtsIMutI CAGTG 7 cut(s) 102, 166, 659, 1229, 2035, 2097, 2316
BtuMI TCGCGA 1 cut(s) 2344
BveI ACCTGC 1 cut(s) 2350
Cac8I GCNNGC 3 cut(s) 279, 1229, 2088
CciI TCATGA 1 cut(s) 118
CfoI GCGC 4 cut(s) 283, 477, 862, 1105
Cfr13I GGNCC 3 cut(s) 689, 722, 1857
CseI GACGC 3 cut(s) 805, 1691, 2270
Csp6I GTAC 8 cut(s) 73, 315, 580, 644, 1441, 1455, 1541, 1768
CspCI CAANNNNNGTGG 2 cut(s) 2181, 2216
CviQI GTAC 8 cut(s) 73, 315, 580, 644, 1441, 1455, 1541, 1768
DinI GGCGCC 1 cut(s) 476
DpnI GATC 5 cut(s) 1296, 1452, 1671, 2130, 2166
DpnII GATC 5 cut(s) 1294, 1450, 1669, 2128, 2164
DraI TTTAAA 2 cut(s) 1462, 1809
Eam1104I CTCTTC 6 cut(s) 308, 387, 515, 821, 1517, 2202
EarI CTCTTC 6 cut(s) 308, 387, 515, 821, 1517, 2202
Ecl136II GAGCTC 1 cut(s) 2212
Eco130I CCWWGG 3 cut(s) 595, 1232, 1879
Eco147I AGGCCT 1 cut(s) 1878
Eco24I GRGCYC 3 cut(s) 371, 833, 2214
Eco31I GGTCTC 1 cut(s) 486
Eco32I GATATC 1 cut(s) 1191
Eco47I GGWCC 3 cut(s) 689, 722, 1857
Eco53kI GAGCTC 1 cut(s) 2212
Eco57I CTGAAG 4 cut(s) 683, 1035, 1144, 2355
EcoICRI GAGCTC 1 cut(s) 2212
EcoNI CCTNNNNNAGG 1 cut(s) 448
EcoO109I RGGNCCY 2 cut(s) 689, 722
EcoRII CCWGG 2 cut(s) 508, 1035
EcoRV GATATC 1 cut(s) 1191
EcoT14I CCWWGG 3 cut(s) 595, 1232, 1879
EcoT38I GRGCYC 3 cut(s) 371, 833, 2214
EgeI GGCGCC 1 cut(s) 476
EheI GGCGCC 1 cut(s) 476
ErhI CCWWGG 3 cut(s) 595, 1232, 1879
FalI AAGNNNNNCTT 6 cut(s) 509, 541, 687, 719, 2243, 2275
FaqI GGGAC 2 cut(s) 675, 1843
FauI CCCGC 1 cut(s) 2079
FauNDI CATATG 1 cut(s) 297
FblI GTMKAC 3 cut(s) 1411, 2177, 2221
Fnu4HI GCNGC 8 cut(s) 19, 177, 180, 219, 222, 225, 247, 1031
FokI GGATG 6 cut(s) 487, 542, 1139, 1192, 1196, 2246
FriOI GRGCYC 3 cut(s) 371, 833, 2214
Fsp4HI GCNGC 8 cut(s) 19, 177, 180, 219, 222, 225, 247, 1031
FspBI CTAG 3 cut(s) 614, 755, 900
FspI TGCGCA 3 cut(s) 282, 861, 1104
GlaI GCGC 4 cut(s) 282, 476, 861, 1104
GluI GCNGC 8 cut(s) 19, 177, 180, 219, 222, 225, 247, 1031
GsaI CCCAGC 1 cut(s) 1864
GsuI CTGGAG 1 cut(s) 1284
HaeII RGCGCY 1 cut(s) 478
HaeIII GGCC 3 cut(s) 99, 150, 1878
HapII CCGG 1 cut(s) 2348
HgaI GACGC 3 cut(s) 805, 1691, 2270
HhaI GCGC 4 cut(s) 283, 477, 862, 1105
Hin1I GRCGYC 1 cut(s) 475
Hin6I GCGC 4 cut(s) 281, 475, 860, 1103
HinP1I GCGC 4 cut(s) 281, 475, 860, 1103
HincII GTYRAC 3 cut(s) 1536, 1629, 2242
HindII GTYRAC 3 cut(s) 1536, 1629, 2242
HindIII AAGCTT 4 cut(s) 86, 743, 1445, 1610
HpaII CCGG 1 cut(s) 2348
HphI GGTGA 3 cut(s) 498, 970, 1942
Hpy166II GTNNAC 6 cut(s) 1412, 1536, 1629, 2178, 2222, 2242
Hpy8I GTNNAC 6 cut(s) 1412, 1536, 1629, 2178, 2222, 2242
Hpy99I CGWCG 1 cut(s) 2286
HpyAV CCTTC 8 cut(s) 208, 488, 713, 1285, 1359, 1565, 1868, 2221
HpyCH4III ACNGT 3 cut(s) 347, 1540, 2414
HpyCH4IV ACGT 2 cut(s) 417, 735
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 751, 2006
HpySE526I ACGT 2 cut(s) 417, 735
Hsp92I GRCGYC 1 cut(s) 475
HspAI GCGC 4 cut(s) 281, 475, 860, 1103
KasI GGCGCC 1 cut(s) 474
Kzo9I GATC 5 cut(s) 1294, 1450, 1669, 2128, 2164
LguI GCTCTTC 5 cut(s) 308, 387, 515, 821, 2202
LmnI GCTCC 3 cut(s) 1027, 1699, 2005
Lsp1109I GCAGC 7 cut(s) 5, 188, 191, 230, 233, 258, 1042
LweI GCATC 4 cut(s) 859, 1330, 1962, 1996
MaeI CTAG 3 cut(s) 614, 755, 900
MaeII ACGT 2 cut(s) 417, 735
MaeIII GTNAC 2 cut(s) 347, 1242
MalI GATC 5 cut(s) 1296, 1452, 1671, 2130, 2166
MbiI CCGCTC 1 cut(s) 2086
MboI GATC 5 cut(s) 1294, 1450, 1669, 2128, 2164
MflI RGATCY 2 cut(s) 1294, 2128
MhlI GDGCHC 4 cut(s) 371, 399, 833, 2214
Mly113I GGCGCC 1 cut(s) 475
MlyI GAGTC 4 cut(s) 977, 1744, 2096, 2360
MmeI TCCRAC 3 cut(s) 217, 718, 2264
MslI CAYNNNNRTG 2 cut(s) 1178, 1721
MspA1I CMGCKG 3 cut(s) 224, 1591, 1619
MspCI CTTAAG 2 cut(s) 1430, 1976
MspI CCGG 1 cut(s) 2348
MspR9I CCNGG 2 cut(s) 510, 1037
Mva1269I GAATGC 1 cut(s) 2295
MvaI CCWGG 2 cut(s) 510, 1037
MvnI CGCG 1 cut(s) 2344
MwoI GCNNNNNNNGC 3 cut(s) 224, 751, 2006
NarI GGCGCC 1 cut(s) 475
NcoI CCATGG 1 cut(s) 595
NdeI CATATG 1 cut(s) 297
NdeII GATC 5 cut(s) 1294, 1450, 1669, 2128, 2164
NlaIV GGNNCC 6 cut(s) 199, 442, 476, 691, 1296, 1859
NmuCI GTSAC 1 cut(s) 1242
NruI TCGCGA 1 cut(s) 2344
NsbI TGCGCA 3 cut(s) 282, 861, 1104
NspI RCATGY 2 cut(s) 1094, 1805
NspV TTCGAA 1 cut(s) 1489
PagI TCATGA 1 cut(s) 118
PceI AGGCCT 1 cut(s) 1878
PciSI GCTCTTC 5 cut(s) 308, 387, 515, 821, 2202
PctI GAATGC 1 cut(s) 2295
PfeI GAWTC 6 cut(s) 632, 1491, 1726, 1776, 2062, 2339
PkrI GCNGC 8 cut(s) 20, 178, 181, 220, 223, 226, 248, 1032
PleI GAGTC 4 cut(s) 977, 1743, 2096, 2359
PluTI GGCGCC 1 cut(s) 478
PpsI GAGTC 4 cut(s) 977, 1743, 2096, 2359
PpuMI RGGWCCY 2 cut(s) 689, 722
PshAI GACNNNNGTC 1 cut(s) 2350
PshBI ATTAAT 2 cut(s) 605, 2123
Psp124BI GAGCTC 1 cut(s) 2214
Psp1406I AACGTT 1 cut(s) 735
Psp5II RGGWCCY 2 cut(s) 689, 722
Psp6I CCWGG 2 cut(s) 508, 1035
PspFI CCCAGC 1 cut(s) 1860
PspGI CCWGG 2 cut(s) 508, 1035
PspN4I GGNNCC 6 cut(s) 199, 442, 476, 691, 1296, 1859
PspPI GGNCC 3 cut(s) 689, 722, 1857
PspPPI RGGWCCY 2 cut(s) 689, 722
PsuI RGATCY 2 cut(s) 1294, 2128
PvuII CAGCTG 2 cut(s) 1591, 1619
RruI TCGCGA 1 cut(s) 2344
RsaI GTAC 8 cut(s) 74, 316, 581, 645, 1442, 1456, 1542, 1769
RsaNI GTAC 8 cut(s) 73, 315, 580, 644, 1441, 1455, 1541, 1768
RseI CAYNNNNRTG 2 cut(s) 1178, 1721
SacI GAGCTC 1 cut(s) 2214
SapI GCTCTTC 5 cut(s) 308, 387, 515, 821, 2202
SatI GCNGC 8 cut(s) 19, 177, 180, 219, 222, 225, 247, 1031
Sau3AI GATC 5 cut(s) 1294, 1450, 1669, 2128, 2164
Sau96I GGNCC 3 cut(s) 689, 722, 1857
SchI GAGTC 4 cut(s) 977, 1744, 2096, 2360
ScrFI CCNGG 2 cut(s) 510, 1037
SduI GDGCHC 4 cut(s) 371, 399, 833, 2214
SfaNI GCATC 4 cut(s) 859, 1330, 1962, 1996
SfcI CTRYAG 1 cut(s) 2222
SfoI GGCGCC 1 cut(s) 476
SfuI TTCGAA 1 cut(s) 1489
SinI GGWCC 3 cut(s) 689, 722, 1857
SmiMI CAYNNNNRTG 2 cut(s) 1178, 1721
SmlI CTYRAG 5 cut(s) 89, 647, 704, 1430, 1976
SmoI CTYRAG 5 cut(s) 89, 647, 704, 1430, 1976
SseBI AGGCCT 1 cut(s) 1878
SsiI CCGC 2 cut(s) 224, 2086
SspDI GGCGCC 1 cut(s) 474
SspI AATATT 2 cut(s) 839, 970
SspMI CTAG 3 cut(s) 614, 755, 900
SstI GAGCTC 1 cut(s) 2214
StuI AGGCCT 1 cut(s) 1878
StyD4I CCNGG 2 cut(s) 508, 1035
StyI CCWWGG 3 cut(s) 595, 1232, 1879
TaaI ACNGT 3 cut(s) 347, 1540, 2414
TaiI ACGT 2 cut(s) 420, 738
TaqI TCGA 3 cut(s) 490, 1489, 1551
TatI WGTACW 5 cut(s) 72, 314, 579, 643, 1540
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 6 cut(s) 632, 1491, 1726, 1776, 2062, 2339
TscAI CASTG 7 cut(s) 109, 166, 659, 1229, 2035, 2097, 2323
TseFI GTSAC 1 cut(s) 1242
TseI GCWGC 7 cut(s) 18, 176, 179, 218, 221, 246, 1030
Tsp45I GTSAC 1 cut(s) 1242
TspDTI ATGAA 8 cut(s) 107, 186, 295, 623, 980, 1434, 1739, 2154
TspGWI ACGGA 1 cut(s) 1260
TspRI CASTG 7 cut(s) 109, 166, 659, 1229, 2035, 2097, 2323
Vha464I CTTAAG 2 cut(s) 1430, 1976
VpaK11BI GGWCC 3 cut(s) 689, 722, 1857
VspI ATTAAT 2 cut(s) 605, 2123
XagI CCTNNNNNAGG 1 cut(s) 448
XapI RAATTY 2 cut(s) 1066, 1651
XceI RCATGY 2 cut(s) 1094, 1805
XmiI GTMKAC 3 cut(s) 1411, 2177, 2221
XspI CTAG 3 cut(s) 614, 755, 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.