Rorug05G0420900

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000183
Physical Location & Seq
Reverse (-)
58509572 .. 58511357
1786 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0420900.1

Sequence Viewer

Length: 1593 bp
ATGTCGGACCCCATTGCCAGCCTTGCTGCTGCCTTTGCCCTCTCTGATGACGCCCACGTCGACCTTAGCTTTGCTGGGGAGGCGGCGCTGCAAGATCCCCATCACTACATGGTGGGACGGTTGTTGACGCGGCGACCAAATACTACAGGTTTTCTTGGTACTATGCCAACGATCTGGAGGATGAGGCAAGGCTTATCCATCAGAGCAGTTGGGGATCGATTTGTTTTTGAGTTTGATAATGAAATTGACAGGAATCGGATTCTGCATGGGGCTCCATGGTTTTATCATAATACTATGCTCTTGTTAGCGCCATATGATGGTATATCTGATGTGGAAGGTATCCCAATTCATTCTCTGGAACTCTGGATTACTGTCAGAGGGCTGCCATTTGGCCTACGTAACAAACTATCTTTGTCCTTGATTGGTTCCGCAATTGGACGGGTTCTCCGTTTCGATCCAGTGGCGCTGAATAGGAAGGATGAAGAGCAGAGGATCCGGTTGGTCGTCGATATTCGTCGGCGTTTTAGGATCTTCCGTAGCTTCTCATTTTCTGCAGTAGTCGCTGTGAATCTCTCGATCGTCTATGAAAAAACTCGAGGATTGTGTAGAGATTGTGGGCTGTTCATGCATTCCACAGATGGCTGTGACAGGTTGATTGCCAATGAAAGGGCGGAACTGCATTCAACTCCGCCGGTACATGCTATGGCGGGTCTATCACTCTTTGCAGCTGGTGGTGGAGCTAATGGCTCGGTAAGAAGTGCTCGGGCTCAGTCTGGAGAGGGTAGGGTTTTGTCAAACCCTAGGAATGGGTGGAATGAATGTAGGGTTTCTTCTAGGGTGGGTTTTCTTCAAGGGGAGGCTGCAGTTAGGGCTACACATCAAGAAAACCAAGCTGCATTCTTTTATACTCATCTTAACTCCGGACTACAGGCAGCTGGCTTGTTGAAGAAGATAGTCAAGCCGATTCCTTTATCTGTGCAGCCTAAGAATGGTCTGGAGAGCAATCAGGTTACTGCTTCTCCTGGTGCTTTATTTATTCAGGCAAGCAAGAATGGGGGTGCTATTGCTCCTAGTTTGGGTAGTGTCCCAGGGGCTCTGAGTGGACTGAAGAGGCTCGCTGAGATCATGATGCGTCCAGGGAAGAAGTTGCTGGCATTGCCTGCACCGAATCTGGCTGGGGTTGCAGAATTATCTCTCTCTCTTCCACAAATGGATGACAGATTGATAATTTCTCCCCAGAAAAAGAAAAAGGCTGGTCGTCCCAAAGGATCCAAAAACAAGGCCAAATTGGTTGGTAATGTGGATGATGTCCTGAGTCCCCGTGTTAAGATCAAGGGAAGGAAACGTTTCCTCAAAAGGCTTGAGGATGAGGTGGAGGTGTCTCATGTTCCTACAGAGAAGGAACCTGCTGAAACTATTGAAGAGGTGATGTCTGCAATGGTTGATTCGGAGTTACCTGTGAAGGGACAGAATGTTGGGAACGAGATGGAGGAGCAGGACGTGGCTTCGGTTTCATCTACAGAGGGTCTGCATCTGGTTGAGGAGGTGATTACCTCAACTGAGGGTGTTGAGACTGTTTCAATTTCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

530

Amino Acids

57.62

Weight (kDa)

9.24

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 35 - 106 4.6e-11 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 56
Acc36I ACCTGC 1 cut(s) 1414
AccB7I CCANNNNNTGG 1 cut(s) 317
AccI GTMKAC 1 cut(s) 60
AccII CGCG 1 cut(s) 130
AccIII TCCGGA 1 cut(s) 920
AciI CCGC 6 cut(s) 83, 130, 429, 671, 689, 707
AclI AACGTT 1 cut(s) 1345
AclWI GGATC 8 cut(s) 89, 222, 449, 487, 500, 536, 1263, 1276
AcuI CTGAAG 1 cut(s) 1127
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 2 cut(s) 160, 696
AfiI CCNNNNNNNGG 6 cut(s) 317, 666, 806, 989, 1076, 1463
AgsI TTSAA 5 cut(s) 684, 851, 946, 1421, 1581
AjiI CACGTC 2 cut(s) 58, 1501
AjnI CCWGG 3 cut(s) 1021, 1087, 1135
AluBI AGCT 6 cut(s) 69, 540, 728, 740, 893, 935
AluI AGCT 6 cut(s) 69, 540, 728, 740, 893, 935
Alw21I GWGCWC 1 cut(s) 763
Alw26I GTCTC 2 cut(s) 1386, 1565
AlwI GGATC 8 cut(s) 89, 222, 449, 487, 500, 536, 1263, 1276
Ama87I CYCGRG 2 cut(s) 594, 762
Aor13HI TCCGGA 1 cut(s) 920
AoxI GGCC 2 cut(s) 391, 1281
ApeKI GCWGC 9 cut(s) 26, 29, 88, 382, 725, 860, 893, 932, 979
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
Asp700I GAANNNNTTC 1 cut(s) 1346
AspA2I CCTAGG 1 cut(s) 800
AspLEI GCGC 3 cut(s) 88, 310, 466
AspS9I GGNCC 1 cut(s) 7
AsuHPI GGTGA 2 cut(s) 1438, 1558
AvaI CYCGRG 2 cut(s) 594, 762
AvaII GGWCC 1 cut(s) 7
AvrII CCTAGG 1 cut(s) 800
BamHI GGATCC 2 cut(s) 492, 1268
BanII GRGCYC 3 cut(s) 274, 769, 1096
BarI GAAGNNNNNNTAC 2 cut(s) 814, 846
Bbv12I GWGCWC 1 cut(s) 763
BbvI GCAGC 9 cut(s) 13, 16, 75, 369, 737, 847, 880, 944, 991
BccI CCATC 5 cut(s) 108, 206, 311, 632, 1480
BciT130I CCWGG 3 cut(s) 1023, 1089, 1137
BciVI GTATCC 1 cut(s) 350
BcoDI GTCTC 2 cut(s) 1386, 1565
BfaI CTAG 3 cut(s) 801, 834, 1071
BfmI CTRYAG 6 cut(s) 144, 552, 861, 926, 1392, 1518
BfoI RGCGCY 3 cut(s) 89, 311, 467
BfuAI ACCTGC 1 cut(s) 1414
BfuI GTATCC 1 cut(s) 350
BlnI CCTAGG 1 cut(s) 800
Bme1390I CCNGG 3 cut(s) 1023, 1089, 1137
Bme18I GGWCC 1 cut(s) 7
BmeT110I CYCGRG 2 cut(s) 594, 762
BmgBI CACGTC 2 cut(s) 58, 1501
BmgT120I GGNCC 1 cut(s) 7
BmiI GGNNCC 6 cut(s) 9, 273, 427, 494, 1270, 1404
BmrFI CCNGG 3 cut(s) 1023, 1089, 1137
BmsI GCATC 2 cut(s) 1119, 1540
BpmI CTGGAG 3 cut(s) 196, 795, 1016
Bpu10I CCTNAGC 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 1382
Bsa29I ATCGAT 1 cut(s) 217
BsaAI YACGTR 1 cut(s) 398
BsaBI GATNNNNATC 1 cut(s) 99
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 5 cut(s) 275, 800, 1087, 1088, 1136
BsaWI WCCGGW 2 cut(s) 495, 920
BsaXI ACNNNNNCTCC 4 cut(s) 429, 459, 1003, 1033
Bsc4I CCNNNNNNNGG 6 cut(s) 317, 666, 806, 989, 1076, 1463
Bse118I RCCGGY 1 cut(s) 691
Bse1I ACTGG 1 cut(s) 458
Bse3DI GCAATG 3 cut(s) 12, 1154, 1443
Bse8I GATNNNNATC 1 cut(s) 99
BseAI TCCGGA 1 cut(s) 920
BseBI CCWGG 3 cut(s) 1023, 1089, 1137
BseCI ATCGAT 1 cut(s) 217
BseDI CCNNGG 5 cut(s) 275, 800, 1087, 1088, 1136
BseGI GGATG 5 cut(s) 186, 484, 1219, 1309, 1372
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 6 cut(s) 317, 666, 806, 989, 1076, 1463
BseMI GCAATG 3 cut(s) 12, 1154, 1443
BseMII CTCAG 5 cut(s) 782, 1088, 1110, 1304, 1551
BseNI ACTGG 1 cut(s) 458
BseRI GAGGAG 2 cut(s) 1505, 1556
BseXI GCAGC 9 cut(s) 13, 16, 75, 369, 737, 847, 880, 944, 991
BseYI CCCAGC 2 cut(s) 74, 1175
BsgI GTGCAG 2 cut(s) 998, 1146
Bsh1236I CGCG 1 cut(s) 130
Bsh1285I CGRYCG 1 cut(s) 579
BshFI GGCC 2 cut(s) 393, 1283
BshVI ATCGAT 1 cut(s) 217
BsiEI CGRYCG 1 cut(s) 579
BsiHKAI GWGCWC 1 cut(s) 763
BsiHKCI CYCGRG 2 cut(s) 594, 762
BsiSI CCGG 3 cut(s) 496, 692, 921
BslFI GGGAC 5 cut(s) 129, 1070, 1245, 1302, 1479
BslI CCNNNNNNNGG 6 cut(s) 317, 666, 806, 989, 1076, 1463
BsmAI GTCTC 2 cut(s) 1386, 1565
BsmFI GGGAC 5 cut(s) 129, 1070, 1245, 1302, 1479
BsmI GAATGC 3 cut(s) 628, 679, 896
BsnI GGCC 2 cut(s) 393, 1283
BsoBI CYCGRG 2 cut(s) 594, 762
Bsp1286I GDGCHC 4 cut(s) 274, 763, 769, 1096
Bsp13I TCCGGA 1 cut(s) 920
Bsp19I CCATGG 1 cut(s) 275
BspACI CCGC 6 cut(s) 83, 130, 429, 671, 689, 707
BspANI GGCC 2 cut(s) 393, 1283
BspCNI CTCAG 5 cut(s) 781, 1089, 1111, 1305, 1552
BspDI ATCGAT 1 cut(s) 217
BspEI TCCGGA 1 cut(s) 920
BspFNI CGCG 1 cut(s) 130
BspHI TCATGA 1 cut(s) 1125
BspLI GGNNCC 6 cut(s) 9, 273, 427, 494, 1270, 1404
BspMAI CTGCAG 2 cut(s) 556, 865
BspMI ACCTGC 1 cut(s) 1414
BspPI GGATC 8 cut(s) 89, 222, 449, 487, 500, 536, 1263, 1276
BspQI GCTCTTC 1 cut(s) 477
BsrDI GCAATG 3 cut(s) 12, 1154, 1443
BsrFI RCCGGY 1 cut(s) 691
BsrI ACTGG 1 cut(s) 458
BssAI RCCGGY 1 cut(s) 691
BssECI CCNNGG 5 cut(s) 275, 800, 1087, 1088, 1136
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 2 cut(s) 275, 800
Bst2UI CCWGG 3 cut(s) 1023, 1089, 1137
Bst4CI ACNGT 3 cut(s) 120, 373, 1576
Bst6I CTCTTC 4 cut(s) 477, 1103, 1206, 1416
BstACI GRCGYC 1 cut(s) 51
BstAPI GCANNNNNTGC 1 cut(s) 1160
BstBAI YACGTR 1 cut(s) 398
BstC8I GCNNGC 6 cut(s) 19, 937, 1045, 1116, 1152, 1161
BstDEI CTNAG 7 cut(s) 65, 768, 984, 1097, 1119, 1313, 1560
BstDSI CCRYGG 1 cut(s) 275
BstF5I GGATG 5 cut(s) 186, 484, 1219, 1309, 1372
BstFNI CGCG 1 cut(s) 130
BstH2I RGCGCY 3 cut(s) 89, 311, 467
BstHHI GCGC 3 cut(s) 88, 310, 466
BstMAI GTCTC 2 cut(s) 1386, 1565
BstMCI CGRYCG 1 cut(s) 579
BstMWI GCNNNNNNNGC 9 cut(s) 23, 35, 80, 560, 625, 869, 1156, 1160, 1181
BstNI CCWGG 3 cut(s) 1023, 1089, 1137
BstNSI RCATGY 1 cut(s) 701
BstSCI CCNGG 3 cut(s) 1021, 1087, 1135
BstSFI CTRYAG 6 cut(s) 144, 552, 861, 926, 1392, 1518
BstSNI TACGTA 1 cut(s) 398
BstUI CGCG 1 cut(s) 130
BstV1I GCAGC 9 cut(s) 13, 16, 75, 369, 737, 847, 880, 944, 991
BstX2I RGATCY 4 cut(s) 94, 492, 528, 1268
BstXI CCANNNNNNTGG 1 cut(s) 174
BstYI RGATCY 4 cut(s) 94, 492, 528, 1268
Bsu15I ATCGAT 1 cut(s) 217
BsuI GTATCC 1 cut(s) 350
BsuRI GGCC 2 cut(s) 393, 1283
BsuTUI ATCGAT 1 cut(s) 217
BtgI CCRYGG 1 cut(s) 275
BtrI CACGTC 2 cut(s) 58, 1501
BtsCI GGATG 5 cut(s) 186, 484, 1219, 1309, 1372
BtsIMutI CAGTG 1 cut(s) 465
BveI ACCTGC 1 cut(s) 1414
Cac8I GCNNGC 6 cut(s) 19, 937, 1045, 1116, 1152, 1161
CciI TCATGA 1 cut(s) 1125
CfoI GCGC 3 cut(s) 88, 310, 466
Cfr10I RCCGGY 1 cut(s) 691
Cfr13I GGNCC 1 cut(s) 7
ClaI ATCGAT 1 cut(s) 217
CseI GACGC 3 cut(s) 59, 136, 1121
Csp6I GTAC 2 cut(s) 159, 695
CviAII CATG 7 cut(s) 109, 266, 276, 625, 698, 1126, 1385
CviQI GTAC 2 cut(s) 159, 695
DdeI CTNAG 7 cut(s) 65, 768, 984, 1097, 1119, 1313, 1560
DrdI GACNNNNNNGTC 1 cut(s) 56
DseDI GACNNNNNNGTC 1 cut(s) 56
Eam1104I CTCTTC 4 cut(s) 477, 1103, 1206, 1416
EarI CTCTTC 4 cut(s) 477, 1103, 1206, 1416
EciI GGCGGA 2 cut(s) 678, 686
Eco105I TACGTA 1 cut(s) 398
Eco130I CCWWGG 2 cut(s) 275, 800
Eco24I GRGCYC 3 cut(s) 274, 769, 1096
Eco47I GGWCC 1 cut(s) 7
Eco57I CTGAAG 1 cut(s) 1127
Eco88I CYCGRG 2 cut(s) 594, 762
EcoRII CCWGG 3 cut(s) 1021, 1087, 1135
EcoT14I CCWWGG 2 cut(s) 275, 800
EcoT22I ATGCAT 1 cut(s) 630
EcoT38I GRGCYC 3 cut(s) 274, 769, 1096
ErhI CCWWGG 2 cut(s) 275, 800
FaeI CATG 7 cut(s) 112, 269, 279, 628, 701, 1129, 1388
FaqI GGGAC 5 cut(s) 129, 1070, 1245, 1302, 1479
FatI CATG 7 cut(s) 108, 265, 275, 624, 697, 1125, 1384
FauI CCCGC 1 cut(s) 700
FauNDI CATATG 1 cut(s) 313
FblI GTMKAC 1 cut(s) 60
FokI GGATG 5 cut(s) 193, 491, 1226, 1316, 1379
FriOI GRGCYC 3 cut(s) 274, 769, 1096
FspBI CTAG 3 cut(s) 801, 834, 1071
GlaI GCGC 3 cut(s) 87, 309, 465
GsaI CCCAGC 2 cut(s) 78, 1179
GsuI CTGGAG 3 cut(s) 196, 795, 1016
HaeII RGCGCY 3 cut(s) 89, 311, 467
HaeIII GGCC 2 cut(s) 393, 1283
HapII CCGG 3 cut(s) 496, 692, 921
HgaI GACGC 3 cut(s) 59, 136, 1121
HhaI GCGC 3 cut(s) 88, 310, 466
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 7 cut(s) 112, 269, 279, 628, 701, 1129, 1388
Hin6I GCGC 3 cut(s) 86, 308, 464
HinP1I GCGC 3 cut(s) 86, 308, 464
HincII GTYRAC 2 cut(s) 61, 126
HindII GTYRAC 2 cut(s) 61, 126
HinfI GANTC 7 cut(s) 253, 259, 568, 964, 1168, 1315, 1445
HpaII CCGG 3 cut(s) 496, 692, 921
HphI GGTGA 2 cut(s) 1438, 1558
Hpy166II GTNNAC 3 cut(s) 61, 126, 1103
Hpy188I TCNGA 8 cut(s) 7, 46, 203, 258, 328, 377, 1098, 1450
Hpy8I GTNNAC 3 cut(s) 61, 126, 1103
Hpy99I CGWCG 3 cut(s) 62, 509, 519
HpyAV CCTTC 5 cut(s) 329, 469, 1332, 1393, 1456
HpyCH4III ACNGT 3 cut(s) 120, 373, 1576
HpyCH4IV ACGT 4 cut(s) 57, 397, 1345, 1500
HpyF10VI GCNNNNNNNGC 9 cut(s) 23, 35, 80, 560, 625, 869, 1156, 1160, 1181
HpyF3I CTNAG 7 cut(s) 65, 768, 984, 1097, 1119, 1313, 1560
HpySE526I ACGT 4 cut(s) 57, 397, 1345, 1500
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 7 cut(s) 112, 269, 279, 628, 701, 1129, 1388
HspAI GCGC 3 cut(s) 86, 308, 464
Kpn2I TCCGGA 1 cut(s) 920
LguI GCTCTTC 1 cut(s) 477
LmnI GCTCC 4 cut(s) 277, 737, 1072, 1492
Lsp1109I GCAGC 9 cut(s) 13, 16, 75, 369, 737, 847, 880, 944, 991
LweI GCATC 2 cut(s) 1119, 1540
MaeI CTAG 3 cut(s) 801, 834, 1071
MaeII ACGT 4 cut(s) 57, 397, 1345, 1500
MaeIII GTNAC 4 cut(s) 398, 644, 1009, 1452
MfeI CAATTG 1 cut(s) 432
MflI RGATCY 4 cut(s) 94, 492, 528, 1268
MhlI GDGCHC 4 cut(s) 274, 763, 769, 1096
MluCI AATT 7 cut(s) 243, 345, 432, 1187, 1227, 1286, 1581
MlyI GAGTC 1 cut(s) 1324
Mph1103I ATGCAT 1 cut(s) 630
MroI TCCGGA 1 cut(s) 920
MroXI GAANNNNTTC 1 cut(s) 1346
MseI TTAA 2 cut(s) 915, 1326
MspA1I CMGCKG 2 cut(s) 728, 935
MspI CCGG 3 cut(s) 496, 692, 921
MspR9I CCNGG 3 cut(s) 1023, 1089, 1137
MunI CAATTG 1 cut(s) 432
Mva1269I GAATGC 3 cut(s) 628, 679, 896
MvaI CCWGG 3 cut(s) 1023, 1089, 1137
MvnI CGCG 1 cut(s) 130
MwoI GCNNNNNNNGC 9 cut(s) 23, 35, 80, 560, 625, 869, 1156, 1160, 1181
NcoI CCATGG 1 cut(s) 275
NdeI CATATG 1 cut(s) 313
NlaIII CATG 7 cut(s) 112, 269, 279, 628, 701, 1129, 1388
NlaIV GGNNCC 6 cut(s) 9, 273, 427, 494, 1270, 1404
NmuCI GTSAC 1 cut(s) 644
NsiI ATGCAT 1 cut(s) 630
NspI RCATGY 1 cut(s) 701
PaeR7I CTCGAG 1 cut(s) 594
PagI TCATGA 1 cut(s) 1125
PasI CCCWGGG 1 cut(s) 1088
PciSI GCTCTTC 1 cut(s) 477
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 445
PctI GAATGC 3 cut(s) 628, 679, 896
PdmI GAANNNNTTC 1 cut(s) 1346
PfeI GAWTC 6 cut(s) 253, 259, 568, 964, 1168, 1445
PflMI CCANNNNNTGG 1 cut(s) 317
Ple19I CGATCG 1 cut(s) 579
PleI GAGTC 1 cut(s) 1323
PpsI GAGTC 1 cut(s) 1323
Ppu21I YACGTR 1 cut(s) 398
Psp1406I AACGTT 1 cut(s) 1345
Psp6I CCWGG 3 cut(s) 1021, 1087, 1135
PspFI CCCAGC 2 cut(s) 74, 1175
PspGI CCWGG 3 cut(s) 1021, 1087, 1135
PspN4I GGNNCC 6 cut(s) 9, 273, 427, 494, 1270, 1404
PspPI GGNCC 1 cut(s) 7
PspXI VCTCGAGB 1 cut(s) 594
PstI CTGCAG 2 cut(s) 556, 865
PsuI RGATCY 4 cut(s) 94, 492, 528, 1268
PvuI CGATCG 1 cut(s) 579
PvuII CAGCTG 2 cut(s) 728, 935
RsaI GTAC 2 cut(s) 160, 696
RsaNI GTAC 2 cut(s) 159, 695
SalI GTCGAC 1 cut(s) 59
SapI GCTCTTC 1 cut(s) 477
SaqAI TTAA 2 cut(s) 915, 1326
Sau96I GGNCC 1 cut(s) 7
SchI GAGTC 1 cut(s) 1324
ScrFI CCNGG 3 cut(s) 1023, 1089, 1137
SduI GDGCHC 4 cut(s) 274, 763, 769, 1096
SfaNI GCATC 2 cut(s) 1119, 1540
SfcI CTRYAG 6 cut(s) 144, 552, 861, 926, 1392, 1518
Sfr274I CTCGAG 1 cut(s) 594
SinI GGWCC 1 cut(s) 7
SlaI CTCGAG 1 cut(s) 594
SmlI CTYRAG 2 cut(s) 594, 1361
SmoI CTYRAG 2 cut(s) 594, 1361
SnaBI TACGTA 1 cut(s) 398
Sse9I AATT 7 cut(s) 243, 345, 432, 1187, 1227, 1286, 1581
SsiI CCGC 6 cut(s) 83, 130, 429, 671, 689, 707
SspMI CTAG 3 cut(s) 801, 834, 1071
StyD4I CCNGG 3 cut(s) 1021, 1087, 1135
StyI CCWWGG 2 cut(s) 275, 800
TaaI ACNGT 3 cut(s) 120, 373, 1576
TaiI ACGT 4 cut(s) 60, 400, 1348, 1503
TaqI TCGA 6 cut(s) 60, 217, 453, 507, 575, 595
TasI AATT 7 cut(s) 243, 345, 432, 1187, 1227, 1286, 1581
TauI GCSGC 2 cut(s) 86, 133
TfiI GAWTC 6 cut(s) 253, 259, 568, 964, 1168, 1445
Tru1I TTAA 2 cut(s) 915, 1326
Tru9I TTAA 2 cut(s) 915, 1326
TscAI CASTG 1 cut(s) 465
TseFI GTSAC 1 cut(s) 644
TseI GCWGC 9 cut(s) 26, 29, 88, 382, 725, 860, 893, 932, 979
Tsp45I GTSAC 1 cut(s) 644
TspDTI ATGAA 8 cut(s) 255, 338, 495, 600, 613, 678, 831, 1503
TspGWI ACGGA 2 cut(s) 437, 524
TspRI CASTG 1 cut(s) 465
Van91I CCANNNNNTGG 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 7
XceI RCATGY 1 cut(s) 701
XhoI CTCGAG 1 cut(s) 594
XmaJI CCTAGG 1 cut(s) 800
XmiI GTMKAC 1 cut(s) 60
XmnI GAANNNNTTC 1 cut(s) 1346
XspI CTAG 3 cut(s) 801, 834, 1071
Zsp2I ATGCAT 1 cut(s) 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.