Rroxscaffold_1G00008160

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000183
Physical Location & Seq
Forward (+)
10289003 .. 10305515
16513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00008160.1

Sequence Viewer

Length: 3354 bp
ATGGAGCGAGCTTATACGAACACCAATATGGATCCTTGGAGTTACTCAACATACTTGAGAGATCAAGTTCTGTTGCAGAAATTCTATACCGTAGATGAGCAGTTGCATGTTTTGAAGGGATTGTCTGTTTCTGATCTGAAGTCGTTCATTCCTGAGATCTTTTCCCAGCTATACATTGAGGGCCTTTTGCATGGCAATTTGTCAGAAGAAGAAGCAATTAGTCTTGCAAATTTATTTCAAACAAATTTTACTGTACCACCACTTCCTACCAAATTGGTGTATAAAGACCATTGTATCTGTCTTCCTCCGAATGCTAACCCTGTTAGAGATGCTACTGTGAAGAACAAGTCAGAAACAAACTCTGTGACTGAGGAAGGGCTGGACGATGATTCCTTTGAGAATTATAAAGGTGGACTAATGGCCAAGGTTTTGGAGAAAGATGCATCCCTCACATCTGAAACCAATCGATTGTGGACTCAGATTCTTGATAAATGGCCGCAGAACAACTCAGAAGCAGTCAGAAGGAGGATGTTATCAACTTTTACAAGACCTATCTGCAACAATCATCTCCAAAGCGTCGAAGACTTGCAACTCGTGTATGGGGTTGCAATCAGCGATTTAAAAGGCGAATGCATGAGGCTAGGCGTTTCCCTTGCATTAAGCCTCAAAGGCATTAAGGCGCTTTTAATGTGCAAGTCATTGAAGACCTTGCAAGAAGCTGAAGAAGCACTACCGGAGTCTGTGCAAGTCATTGAAGACTTTGCAGCCTTTAAGATGTCGTCCAAGTTTTATGATTCTCCTAATGATAAACGACTCTACAGAGTGATTAAGCTTGAGAATGGCCTCACTGCATTGCTCATTCACGATCCTCAGGTCTACCCACAAGGACCACCCCACCACTCCTCCGACCTTGAACAACAACAACAAGATAGTGAAGCTGTAGAAGAAGAAGAGGAAGAGGAAGAAGAAGAAGAAGAAGATAGTGAAGCTGTAGAAGAAGAAAAGGAAGAGGAAGGTGAAGTTAAAAGGAAGGAAGAGGGAGGTGCTTCTGAGACTAAGAAGGCAGCAGCAGCAATGTGTGTCGGAATAGGCAGCTTCTCTGACCCTCTCGAGGCTCAGGGGCTTGCACATTTTCTAGAGTTTAAAACGGTTGTGCAAAACGATTTCTGCCGCCTTCGACAACTTCAGGGCCATACATCCTCACCTGGTCACCCATTTAATAAATTCTCTTGGGGAAATAAGAAGAGCTTGGATGATGCAAAGGAAAAAGGGATCAACTTGCAGGAACGAATACTAAAATTGTACAGAGACTATTACCACGGTGGATTAATGAATTTAGTTGTCATTGGTGGAGAATCTCTTGATGTACTTGAGCACTGGGTTTTGGAATTGTTTGGAGATGTCAAAAAAGGTCCCCAAGTAAATCTGGAGTTCAAGGCAGAAGGTCCTATTTGGAAAGCTGGAAAACTTTACAGGCTAGAGGCTGTTGACGATGTTCATATACTCCACCTAGCATGGACACTTCCATGTCTTCAAGAACACTATTTGAAGAAACCAGAAGATTACTTGAGTCATCTGCTTGGGCATGAGGGCAGGGGAAGTTTGCATTTCTACCTGAAAGCTAGAGGGTGGGTAACATCTTTAGATACTTGTTTGAGTGGGATGGACTGCTCTTCTGTGGCTTATATCTTTTGCATGGTCATATACCTCACTGACTCTGGATTGGAGAAGATTTTTGAAATAATTGGGTTCGTGTATCAATACATTGAGTTATTGCGTCAAATATCACCACAAGAATGGATATTTCGGGAACTTCAGGATATTGGGAACATGGAATTTAGATTTGCAGAGGAGCAGGATCAGGATGATTATGCTTCACAACTTGCAGAAAATTTACTATATTATGCAACAGAGCATGTTATTTATGGGGACTATGTGAATGAGAGTTGGGACAAGGAATTGATAGAATATGTTCATGGTTTCTTCAGACCAGAAAACATGAGGATTGATGTGATATCAAAGTCCTTGGTTAAGTCAGAAGATTTCCAGTGTGAGCCTTGGTTTGGATCACATTATACTGAGGAAGATATATCTCCATCTTTAATAAATTTATGGAAGAATCCTCAAGAAATTAGTGTTTCATTGCATCTGCCAGCAAAGAATGATTTCATTCCTCGTGATTTCTCCATCCGTTCTGATGTTTTGTGCATTGATACTGCAACGACATCTTATCCGAGATGTATACTTGATGAACCATTGATGAAGTTTTGGTACAAGCTCGACAGTACATTTAAACTTCCACATGTAAATACATATTTCCACATCAATCTGAAGGGTGCATGTGATGATGTGAAGAGTTGTGTTTTGACTGGCTTATACCTTGACCTTCTCACAGATCAGCTGAATGAGATCCTCTATGAGGCCTGTGTTGCCAGCTTGGGAACTTCTTTTTCTCTGTCTCTGGACAAACTGCAGTTAGAGGTATACGGTTTCAATGATAAGCTTCCAGCTCTGTTGTCAAAAATTTTGGAAACAGTAAAAAGTTTCTTGCCAACTGATGATCGTTTTGAGGTTTTTAAAGAAGACATGAAGCGAGCATATACGAACACCAATATGGATCCTTGGAGTTACTCAACATATTTGAGAGATCAAGTTCTGTTGAAGAAATTCTATACCGTAGATGAGCAGTTGCAAGTTTTGAAGGGATTGTCTGTTTCTGATCTGAAGTCTTTCATTCCTGAGATTTTTTCCCAGCTATACATTGAGGGCCTTTTGCATGGCAACTTGTCAGAAGAAGAAGCTATTAGTCTTGCAAAATTATTTCAAACAAATTTTACTGTACCACCACTTCCTAGCGAGTTGGTGTATAGAGACAATTGTATCTGTCTTCCTCCAAATGCTAACCTCATTAGAGATGCTACTGTGAAGAACAAGTCAGAAACAAACTCTGTGACTGAGGAAGGACTGGAAGATGATTCCTTTGAGAATTATAAAGGCGGACTAATGGCAAAGATTCTGGAGAAAGATGCATCCCTCACATGTGAAACCAATCGATTGTGGACTCAGATTCTTGCTAAATGGTACAAGTTTGACTATTCGAAAAAGGCTGCAGAACAACTCAGAAGCATTCAGAAGGAGGATGTTACCAAATTTTACAAGACCTATTTGCAACAATCATCTCCAAAGCGTCGAAGACTTGCAACTCGTGTTTGGGGTTGCAACACTGACTTGAAAGAAGCTGAAGAAGCGCGACCGGAGTCTGTGCAAGTCATTGAAGACCTTGCGGCCTTTAAGATGTCGTCCAAGTTTTATGATCGCAAAAGATGTCAAAAAGTGACTCCTCTAAATAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

1117

Amino Acids

128.12

Weight (kDa)

4.94

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16_C PF05193 429 - 606 2.5e-17 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 613 - 898 2e-84 Middle or third domain of peptidase_M16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 405, 2991
AccI GTMKAC 3 cut(s) 876, 2242, 2484
AccII CGCG 1 cut(s) 3250
AciI CCGC 4 cut(s) 497, 1171, 2997, 3284
AclWI GGATC 9 cut(s) 26, 39, 860, 1280, 1866, 2074, 2404, 2612, 2625
AcoI YGGCCR 2 cut(s) 420, 494
AcuI CTGAAG 8 cut(s) 158, 741, 1169, 1799, 1969, 2351, 2744, 3261
AfaI GTAC 7 cut(s) 255, 1304, 1368, 2273, 2287, 2841, 3083
AfiI CCNNNNNNNGG 3 cut(s) 1111, 2063, 2419
AflIII ACRYGT 2 cut(s) 2302, 3038
AjnI CCWGG 1 cut(s) 1204
Alw21I GWGCWC 1 cut(s) 1377
Alw26I GTCTC 4 cut(s) 1046, 1302, 2463, 2865
AlwI GGATC 9 cut(s) 26, 39, 860, 1280, 1866, 2074, 2404, 2612, 2625
Ama87I CYCGRG 1 cut(s) 1109
AoxI GGCC 8 cut(s) 181, 420, 494, 841, 1189, 2421, 2767, 3285
ApeKI GCWGC 6 cut(s) 764, 1064, 1067, 1070, 1092, 3107
AseI ATTAAT 1 cut(s) 1328
Asp700I GAANNNNTTC 5 cut(s) 143, 1971, 2165, 2666, 2729
AspLEI GCGC 2 cut(s) 682, 3250
AspS9I GGNCC 6 cut(s) 181, 887, 1189, 1412, 1445, 2767
AsuHPI GGTGA 4 cut(s) 1028, 1194, 1202, 1779
AsuII TTCGAA 1 cut(s) 3098
AvaI CYCGRG 1 cut(s) 1109
AvaII GGWCC 3 cut(s) 887, 1412, 1445
AxyI CCTNAGG 1 cut(s) 870
BaeI ACNNNNGTAYC 4 cut(s) 277, 310, 2863, 2896
BalI TGGCCA 1 cut(s) 422
BamHI GGATCC 2 cut(s) 31, 2617
BarI GAAGNNNNNNTAC 8 cut(s) 246, 278, 714, 746, 2255, 2287, 2832, 2864
BauI CACGAG 3 cut(s) 593, 2175, 3204
BbsI GAAGAC 9 cut(s) 293, 588, 710, 762, 1523, 2589, 2879, 3199, 3282
Bbv12I GWGCWC 1 cut(s) 1377
BbvI GCAGC 6 cut(s) 776, 1076, 1079, 1082, 1104, 3094
BccI CCATC 3 cut(s) 1657, 2104, 2195
BciT130I CCWGG 1 cut(s) 1206
BcoDI GTCTC 4 cut(s) 1046, 1302, 2463, 2865
BfaI CTAG 6 cut(s) 641, 1136, 1478, 1511, 1623, 2853
BfmI CTRYAG 5 cut(s) 817, 939, 990, 2471, 3108
BfoI RGCGCY 1 cut(s) 683
BglI GCCNNNNNGGC 1 cut(s) 669
BglII AGATCT 1 cut(s) 156
BisI GCNGC 9 cut(s) 497, 765, 1065, 1068, 1071, 1093, 1171, 3108, 3285
BlsI GCNGC 9 cut(s) 498, 766, 1066, 1069, 1072, 1094, 1172, 3109, 3286
Bme1390I CCNGG 1 cut(s) 1206
Bme18I GGWCC 3 cut(s) 887, 1412, 1445
BmeT110I CYCGRG 1 cut(s) 1109
BmgT120I GGNCC 6 cut(s) 181, 887, 1189, 1412, 1445, 2767
BmiI GGNNCC 3 cut(s) 33, 1414, 2619
BmrFI CCNGG 1 cut(s) 1206
BmrI ACTGGG 1 cut(s) 1387
BmsI GCATC 8 cut(s) 319, 430, 452, 1246, 2155, 2905, 3016, 3038
BmuI ACTGGG 1 cut(s) 1387
BoxI GACNNNNGTC 1 cut(s) 3256
BpiI GAAGAC 9 cut(s) 293, 588, 710, 762, 1523, 2589, 2879, 3199, 3282
BpmI CTGGAG 2 cut(s) 1448, 3038
Bpu10I CCTNAGC 1 cut(s) 1116
Bpu14I TTCGAA 1 cut(s) 3098
BpuEI CTTGAG 5 cut(s) 76, 854, 1391, 1588, 2109
Bsa29I ATCGAT 2 cut(s) 466, 3052
BsaJI CCNNGG 6 cut(s) 35, 423, 1318, 2025, 2057, 2621
BsaWI WCCGGW 2 cut(s) 733, 3253
BsaXI ACNNNNNCTCC 2 cut(s) 887, 917
Bsc4I CCNNNNNNNGG 3 cut(s) 1111, 2063, 2419
Bse1I ACTGG 4 cut(s) 1382, 2047, 2374, 2970
Bse21I CCTNAGG 1 cut(s) 870
Bse3DI GCAATG 3 cut(s) 851, 1080, 2141
BseBI CCWGG 1 cut(s) 1206
BseCI ATCGAT 2 cut(s) 466, 3052
BseDI CCNNGG 6 cut(s) 35, 423, 1318, 2025, 2057, 2621
BseGI GGATG 9 cut(s) 443, 534, 1196, 1258, 1668, 1870, 2187, 3029, 3145
BseLI CCNNNNNNNGG 3 cut(s) 1111, 2063, 2419
BseMI GCAATG 3 cut(s) 851, 1080, 2141
BseNI ACTGG 4 cut(s) 1382, 2047, 2374, 2970
BseRI GAGGAG 3 cut(s) 892, 1865, 3330
BseXI GCAGC 6 cut(s) 776, 1076, 1079, 1082, 1104, 3094
BseYI CCCAGC 2 cut(s) 165, 2751
Bsh1236I CGCG 1 cut(s) 3250
Bsh1285I CGRYCG 1 cut(s) 3254
BshFI GGCC 8 cut(s) 183, 422, 496, 843, 1191, 2423, 2769, 3287
BshVI ATCGAT 2 cut(s) 466, 3052
BsiEI CGRYCG 1 cut(s) 3254
BsiHKAI GWGCWC 1 cut(s) 1377
BsiHKCI CYCGRG 1 cut(s) 1109
BsiSI CCGG 2 cut(s) 734, 3254
BslFI GGGAC 3 cut(s) 1398, 1943, 1964
BslI CCNNNNNNNGG 3 cut(s) 1111, 2063, 2419
BsmAI GTCTC 4 cut(s) 1046, 1302, 2463, 2865
BsmFI GGGAC 3 cut(s) 1398, 1943, 1964
BsmI GAATGC 3 cut(s) 316, 635, 3126
BsnI GGCC 8 cut(s) 183, 422, 496, 843, 1191, 2423, 2769, 3287
BsoBI CYCGRG 1 cut(s) 1109
Bsp119I TTCGAA 1 cut(s) 3098
Bsp1286I GDGCHC 1 cut(s) 1377
Bsp1407I TGTACA 1 cut(s) 1302
BspACI CCGC 4 cut(s) 497, 1171, 2997, 3284
BspANI GGCC 8 cut(s) 183, 422, 496, 843, 1191, 2423, 2769, 3287
BspDI ATCGAT 2 cut(s) 466, 3052
BspFNI CGCG 1 cut(s) 3250
BspLI GGNNCC 3 cut(s) 33, 1414, 2619
BspMAI CTGCAG 2 cut(s) 2475, 3112
BspPI GGATC 9 cut(s) 26, 39, 860, 1280, 1866, 2074, 2404, 2612, 2625
BspQI GCTCTTC 2 cut(s) 1238, 1678
BspT104I TTCGAA 1 cut(s) 3098
BsrDI GCAATG 3 cut(s) 851, 1080, 2141
BsrGI TGTACA 1 cut(s) 1302
BsrI ACTGG 4 cut(s) 1382, 2047, 2374, 2970
BssECI CCNNGG 6 cut(s) 35, 423, 1318, 2025, 2057, 2621
BssNAI GTATAC 2 cut(s) 2243, 2485
BssSI CACGAG 3 cut(s) 593, 2175, 3204
BssT1I CCWWGG 5 cut(s) 35, 423, 2025, 2057, 2621
Bst1107I GTATAC 2 cut(s) 2243, 2485
Bst2BI CACGAG 3 cut(s) 593, 2175, 3204
Bst2UI CCWGG 1 cut(s) 1206
Bst6I CTCTTC 7 cut(s) 945, 951, 1002, 1029, 1238, 1678, 2348
BstAUI TGTACA 1 cut(s) 1302
BstBI TTCGAA 1 cut(s) 3098
BstC8I GCNNGC 5 cut(s) 9, 1125, 2154, 2434, 2595
BstDSI CCRYGG 1 cut(s) 1318
BstEII GGTNACC 1 cut(s) 1208
BstENI CCTNNNNNAGG 1 cut(s) 2417
BstF5I GGATG 9 cut(s) 443, 534, 1196, 1258, 1668, 1870, 2187, 3029, 3145
BstFNI CGCG 1 cut(s) 3250
BstH2I RGCGCY 1 cut(s) 683
BstHHI GCGC 2 cut(s) 682, 3250
BstMAI GTCTC 4 cut(s) 1046, 1302, 2463, 2865
BstMCI CGRYCG 1 cut(s) 3254
BstMWI GCNNNNNNNGC 5 cut(s) 669, 725, 1070, 2429, 3245
BstNI CCWGG 1 cut(s) 1206
BstNSI RCATGY 5 cut(s) 110, 1919, 2306, 2343, 3042
BstPAI GACNNNNGTC 1 cut(s) 3256
BstPI GGTNACC 1 cut(s) 1208
BstSCI CCNGG 1 cut(s) 1204
BstSFI CTRYAG 5 cut(s) 817, 939, 990, 2471, 3108
BstUI CGCG 1 cut(s) 3250
BstV1I GCAGC 6 cut(s) 776, 1076, 1079, 1082, 1104, 3094
BstV2I GAAGAC 9 cut(s) 293, 588, 710, 762, 1523, 2589, 2879, 3199, 3282
BstX2I RGATCY 4 cut(s) 31, 156, 2409, 2617
BstXI CCANNNNNNTGG 2 cut(s) 430, 1797
BstYI RGATCY 4 cut(s) 31, 156, 2409, 2617
BstZ17I GTATAC 2 cut(s) 2243, 2485
Bsu15I ATCGAT 2 cut(s) 466, 3052
Bsu36I CCTNAGG 1 cut(s) 870
BsuRI GGCC 8 cut(s) 183, 422, 496, 843, 1191, 2423, 2769, 3287
BsuTUI ATCGAT 2 cut(s) 466, 3052
BtgI CCRYGG 1 cut(s) 1318
BtsCI GGATG 9 cut(s) 443, 534, 1196, 1258, 1668, 1870, 2187, 3029, 3145
BtsI GCAGTG 1 cut(s) 846
BtsIMutI CAGTG 5 cut(s) 846, 1375, 1710, 2054, 3222
Cac8I GCNNGC 5 cut(s) 9, 1125, 2154, 2434, 2595
CfoI GCGC 2 cut(s) 682, 3250
Cfr13I GGNCC 6 cut(s) 181, 887, 1189, 1412, 1445, 2767
ClaI ATCGAT 2 cut(s) 466, 3052
CseI GACGC 3 cut(s) 565, 1766, 3176
CsiI ACCWGGT 1 cut(s) 1204
Csp6I GTAC 7 cut(s) 254, 1303, 1367, 2272, 2286, 2840, 3082
CviQI GTAC 7 cut(s) 254, 1303, 1367, 2272, 2286, 2840, 3082
DraI TTTAAA 4 cut(s) 621, 1144, 2293, 2578
EaeI YGGCCR 2 cut(s) 420, 494
Eam1104I CTCTTC 7 cut(s) 945, 951, 1002, 1029, 1238, 1678, 2348
EarI CTCTTC 7 cut(s) 945, 951, 1002, 1029, 1238, 1678, 2348
EciI GGCGGA 1 cut(s) 3012
Eco130I CCWWGG 5 cut(s) 35, 423, 2025, 2057, 2621
Eco147I AGGCCT 1 cut(s) 2423
Eco32I GATATC 1 cut(s) 2016
Eco47I GGWCC 3 cut(s) 887, 1412, 1445
Eco57I CTGAAG 8 cut(s) 158, 741, 1169, 1799, 1969, 2351, 2744, 3261
Eco81I CCTNAGG 1 cut(s) 870
Eco88I CYCGRG 1 cut(s) 1109
Eco91I GGTNACC 1 cut(s) 1208
EcoNI CCTNNNNNAGG 1 cut(s) 2417
EcoO109I RGGNCCY 4 cut(s) 181, 1412, 1445, 2767
EcoO65I GGTNACC 1 cut(s) 1208
EcoRII CCWGG 1 cut(s) 1204
EcoRV GATATC 1 cut(s) 2016
EcoT14I CCWWGG 5 cut(s) 35, 423, 2025, 2057, 2621
EcoT22I ATGCAT 3 cut(s) 445, 635, 3031
ErhI CCWWGG 5 cut(s) 35, 423, 2025, 2057, 2621
FalI AAGNNNNNCTT 2 cut(s) 1232, 1264
FaqI GGGAC 3 cut(s) 1398, 1943, 1964
FblI GTMKAC 3 cut(s) 876, 2242, 2484
Fnu4HI GCNGC 9 cut(s) 497, 765, 1065, 1068, 1071, 1093, 1171, 3108, 3285
FokI GGATG 9 cut(s) 430, 541, 1183, 1265, 1675, 1877, 2174, 3016, 3152
Fsp4HI GCNGC 9 cut(s) 497, 765, 1065, 1068, 1071, 1093, 1171, 3108, 3285
FspBI CTAG 6 cut(s) 641, 1136, 1478, 1511, 1623, 2853
GlaI GCGC 2 cut(s) 681, 3249
GluI GCNGC 9 cut(s) 497, 765, 1065, 1068, 1071, 1093, 1171, 3108, 3285
GsaI CCCAGC 2 cut(s) 169, 2755
GsuI CTGGAG 2 cut(s) 1448, 3038
HaeII RGCGCY 1 cut(s) 683
HaeIII GGCC 8 cut(s) 183, 422, 496, 843, 1191, 2423, 2769, 3287
HapII CCGG 2 cut(s) 734, 3254
HgaI GACGC 3 cut(s) 565, 1766, 3176
HhaI GCGC 2 cut(s) 682, 3250
Hin6I GCGC 2 cut(s) 680, 3248
HinP1I GCGC 2 cut(s) 680, 3248
HincII GTYRAC 1 cut(s) 1489
HindII GTYRAC 1 cut(s) 1489
HindIII AAGCTT 2 cut(s) 830, 2501
HpaII CCGG 2 cut(s) 734, 3254
HphI GGTGA 4 cut(s) 1028, 1194, 1202, 1779
Hpy166II GTNNAC 7 cut(s) 413, 474, 877, 1489, 2243, 2485, 3060
Hpy8I GTNNAC 7 cut(s) 413, 474, 877, 1489, 2243, 2485, 3060
Hpy99I CGWCG 2 cut(s) 581, 3192
HpyF10VI GCNNNNNNNGC 5 cut(s) 669, 725, 1070, 2429, 3245
HspAI GCGC 2 cut(s) 680, 3248
LguI GCTCTTC 2 cut(s) 1238, 1678
LmnI GCTCC 2 cut(s) 4, 1852
Lsp1109I GCAGC 6 cut(s) 776, 1076, 1079, 1082, 1104, 3094
LweI GCATC 8 cut(s) 319, 430, 452, 1246, 2155, 2905, 3016, 3038
MabI ACCWGGT 1 cut(s) 1204
MaeI CTAG 6 cut(s) 641, 1136, 1478, 1511, 1623, 2853
MaeIII GTNAC 8 cut(s) 41, 364, 1208, 1633, 2627, 2950, 3142, 3334
MfeI CAATTG 1 cut(s) 2875
MflI RGATCY 4 cut(s) 31, 156, 2409, 2617
MhlI GDGCHC 1 cut(s) 1377
MlsI TGGCCA 1 cut(s) 422
MluNI TGGCCA 1 cut(s) 422
MlyI GAGTC 8 cut(s) 469, 746, 807, 1579, 1709, 3055, 3266, 3331
MmeI TCCRAC 2 cut(s) 930, 1063
Mox20I TGGCCA 1 cut(s) 422
Mph1103I ATGCAT 3 cut(s) 445, 635, 3031
MroXI GAANNNNTTC 5 cut(s) 143, 1971, 2165, 2666, 2729
MscI TGGCCA 1 cut(s) 422
MslI CAYNNNNRTG 4 cut(s) 26, 1525, 1795, 2612
Msp20I TGGCCA 1 cut(s) 422
MspA1I CMGCKG 1 cut(s) 2401
MspI CCGG 2 cut(s) 734, 3254
MspR9I CCNGG 1 cut(s) 1206
MunI CAATTG 1 cut(s) 2875
Mva1269I GAATGC 3 cut(s) 316, 635, 3126
MvaI CCWGG 1 cut(s) 1206
MvnI CGCG 1 cut(s) 3250
MwoI GCNNNNNNNGC 5 cut(s) 669, 725, 1070, 2429, 3245
NlaIV GGNNCC 3 cut(s) 33, 1414, 2619
NmuCI GTSAC 4 cut(s) 364, 1208, 2950, 3334
NsiI ATGCAT 3 cut(s) 445, 635, 3031
NspI RCATGY 5 cut(s) 110, 1919, 2306, 2343, 3042
NspV TTCGAA 1 cut(s) 3098
PaeR7I CTCGAG 1 cut(s) 1109
PceI AGGCCT 1 cut(s) 2423
PciI ACATGT 2 cut(s) 2302, 3038
PciSI GCTCTTC 2 cut(s) 1238, 1678
PctI GAATGC 3 cut(s) 316, 635, 3126
PdmI GAANNNNTTC 5 cut(s) 143, 1971, 2165, 2666, 2729
PfeI GAWTC 8 cut(s) 389, 481, 794, 1355, 2119, 2975, 3013, 3067
PkrI GCNGC 9 cut(s) 498, 766, 1066, 1069, 1072, 1094, 1172, 3109, 3286
PleI GAGTC 8 cut(s) 469, 745, 807, 1578, 1709, 3055, 3265, 3331
PpsI GAGTC 8 cut(s) 469, 745, 807, 1578, 1709, 3055, 3265, 3331
PpuMI RGGWCCY 2 cut(s) 1412, 1445
PscI ACATGT 2 cut(s) 2302, 3038
PshAI GACNNNNGTC 1 cut(s) 3256
PshBI ATTAAT 1 cut(s) 1328
PsiI TTATAA 2 cut(s) 405, 2991
Psp5II RGGWCCY 2 cut(s) 1412, 1445
Psp6I CCWGG 1 cut(s) 1204
PspEI GGTNACC 1 cut(s) 1208
PspFI CCCAGC 2 cut(s) 165, 2751
PspGI CCWGG 1 cut(s) 1204
PspN4I GGNNCC 3 cut(s) 33, 1414, 2619
PspPI GGNCC 6 cut(s) 181, 887, 1189, 1412, 1445, 2767
PspPPI RGGWCCY 2 cut(s) 1412, 1445
PstI CTGCAG 2 cut(s) 2475, 3112
PsuI RGATCY 4 cut(s) 31, 156, 2409, 2617
PvuII CAGCTG 1 cut(s) 2401
RsaI GTAC 7 cut(s) 255, 1304, 1368, 2273, 2287, 2841, 3083
RsaNI GTAC 7 cut(s) 254, 1303, 1367, 2272, 2286, 2840, 3082
RseI CAYNNNNRTG 4 cut(s) 26, 1525, 1795, 2612
SapI GCTCTTC 2 cut(s) 1238, 1678
SatI GCNGC 9 cut(s) 497, 765, 1065, 1068, 1071, 1093, 1171, 3108, 3285
Sau96I GGNCC 6 cut(s) 181, 887, 1189, 1412, 1445, 2767
SchI GAGTC 8 cut(s) 469, 746, 807, 1579, 1709, 3055, 3266, 3331
ScrFI CCNGG 1 cut(s) 1206
SduI GDGCHC 1 cut(s) 1377
SexAI ACCWGGT 1 cut(s) 1204
SfaNI GCATC 8 cut(s) 319, 430, 452, 1246, 2155, 2905, 3016, 3038
SfcI CTRYAG 5 cut(s) 817, 939, 990, 2471, 3108
Sfr274I CTCGAG 1 cut(s) 1109
SfuI TTCGAA 1 cut(s) 3098
SinI GGWCC 3 cut(s) 887, 1412, 1445
SlaI CTCGAG 1 cut(s) 1109
SmiMI CAYNNNNRTG 4 cut(s) 26, 1525, 1795, 2612
SmlI CTYRAG 6 cut(s) 55, 833, 1109, 1370, 1567, 2124
SmoI CTYRAG 6 cut(s) 55, 833, 1109, 1370, 1567, 2124
SseBI AGGCCT 1 cut(s) 2423
SsiI CCGC 4 cut(s) 497, 1171, 2997, 3284
SspMI CTAG 6 cut(s) 641, 1136, 1478, 1511, 1623, 2853
StuI AGGCCT 1 cut(s) 2423
StyD4I CCNGG 1 cut(s) 1204
StyI CCWWGG 5 cut(s) 35, 423, 2025, 2057, 2621
TaqI TCGA 8 cut(s) 466, 579, 1110, 1177, 2280, 3052, 3098, 3190
TatI WGTACW 3 cut(s) 1302, 1366, 2285
TauI GCSGC 3 cut(s) 499, 1173, 3287
TfiI GAWTC 8 cut(s) 389, 481, 794, 1355, 2119, 2975, 3013, 3067
TscAI CASTG 5 cut(s) 853, 1382, 1717, 2054, 3229
TseFI GTSAC 4 cut(s) 364, 1208, 2950, 3334
TseI GCWGC 6 cut(s) 764, 1064, 1067, 1070, 1092, 3107
Tsp45I GTSAC 4 cut(s) 364, 1208, 2950, 3334
TspGWI ACGGA 1 cut(s) 2180
TspRI CASTG 5 cut(s) 853, 1382, 1717, 2054, 3229
VpaK11BI GGWCC 3 cut(s) 887, 1412, 1445
VspI ATTAAT 1 cut(s) 1328
XagI CCTNNNNNAGG 1 cut(s) 2417
XbaI TCTAGA 1 cut(s) 1135
XceI RCATGY 5 cut(s) 110, 1919, 2306, 2343, 3042
XhoI CTCGAG 1 cut(s) 1109
XmiI GTMKAC 3 cut(s) 876, 2242, 2484
XmnI GAANNNNTTC 5 cut(s) 143, 1971, 2165, 2666, 2729
XspI CTAG 6 cut(s) 641, 1136, 1478, 1511, 1623, 2853
Zsp2I ATGCAT 3 cut(s) 445, 635, 3031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.