Rh5BG497600

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000183
Physical Location & Seq
Reverse (-)
79303952 .. 79311910
7959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG497600.1

Sequence Viewer

Length: 2208 bp
ATGGGTCGCTGCACTTTCTCCTCGGACGATATTGTAATAAAGTCTCCGAATGATAAGAAACTGTACAGACTAATTAAGCTTGAGAATGGCCTCACTGCATTGCTCATTCATGACCCTGAGATATACCCACAAGGATCCAAGAGCCTTGAACCCAGTGAAGAAGATGAAGCAGCAGCTAAAGGTGGAGATGGTGCCTCAGAGACTAAGAAGGCAGCAGCGGCAATGTGCGTTGGAATGGGCAGCTTCTCTGACCCTATGGAAGCACAGGGGCTTGCGCACTTTCTTGAACATATGCTCTTCATGGGGAGTACAAAGTTTCCGGTTGAAAATGAGTATAACAGTTACTTGTCCAAGCATGGGGGCTCATCAAATGCTTACACAAAGGAAGAGCACACTTGCTACCATTTTGACGTGAAACGAGATTTTCTTAAAGGTGCCTTGAAAAGATTTTCTCAGTTTTTTGTTTCACCTCTAATGAGAAGTGAAGCCATGGAGCGAGAGGTACAGTCTATAGATTCAGAGTTTAACAGGGTTCTGCATAGCGATTTGTGGCGCCTTCAACAACTTCGAGACCATACATCCTCACCTGGTCACCCATTTAATAAATTCTCTTGGGAGAGCAGGGGAGGTTTGCAGTTCTACCTCAAAACTAGAGGGTGGGCAGTATCTCTAAGTGCTTGGGTGGAGAGCTATTCTGTGGCTTATGTCTTTGGCATGAATATTTACCTCACCGACTCTGGATTGGAGAAGATTTTTGAAATTATTGGGTTCGTGTATCAATACATTAAACTATTGCGTCAAGTGTCACCACAAAAATGGATATTTCAAGAACTCCAGGATATTCGGAACATACACTTTAGATTTGTAGAGGAGCAGCCCCAGGATGACTATGCTTCAGAACTTGCAGGAAATTTACTACTTTATCCAGCAGAGCATGTTATTTCTGCGCAATGTGGGTATGAGATTTGGGATGAGGAATTGATTGAATATGTTCTTAGTTTCTTCAGACCAGACAACATGAGGATGGATGTGATATCAAAGTTTTCAACTATTTCCAAAGATTTTCAGTGCGAGCCTTGGTTTGGTTCACATTATATTGAGGAAGATGTATCTCCGTTTTTGATGGATTTGTGGAAGGATCCTCCAGAGATTGATGTTTCGTTGCATCTCCGAGAAAAGAATGAGTTTATTCCTTCTGATTTTTCCATTCGTTCTGATGGTCTTGATACTACATATACATCTTCTCCGAGATGTATACTTGATGAACCTTTGCTTAAGTTCTGGTACAAGCTTGATCGTACTTTTAAAGTTCCACGGGCAAATATATACTTTCGAATCAGTCTAAAGGGTGGATTTGATGACTTGAAGAGTTCTCTGTTGACTGTACTATATATCGACCTTCTTAGAGATGAGCTGAATGAGATTTTATATCAGGCCAGTTTTGCCCTGCTGGATACTTCTTTGTATGTGAATAATTGCTATCTGGAACTGAAGCTGTGGGGCTTCAATGACAAGCTTCCAGCTGTATTGTCAACAATTCTGACAACAACCAAAAATTTCCTGCCAACTTTTGATCGTTTCAAGGTTATTAAAGAAAATATGCAGCGTCTGATAAAGAACACTAATATGAATCCTCTGAGTCATGCTGAATACTTGAAACTGCAAGTTTTGTACCAGAGATTCTACGATGTAGATGACCAGTTGCATGTTTTAAATGGATTGTCTGTTTCTGATGTGAAGTCATTTATTCCAGAGCTTTGGTCCCAGTCGTCGGAGTACAACCCAATCTACTTGCAGCGGAGAGTTGACAACTTTATCAATGGTCTGGAAGACATTTTGCAAGGACTGGATGATGATTCCTTTGAGAATTACAGAGGTGGGCTAATGGCAAAACTATTGGTGAAAAATTCATCCCTTATACATGAAACCAATAGATTTTGGAATCAGATTATTCATAAAAGGTATACATTTGACTATGCAAAGAGGGTGGCAGAAGAGCTCAGCAGTCTACAGAAGGAGGATGTTGTCAACTTTTACAAGACCTACTTGCAACAATCATCTCCCAAGCGTCGGAGACTTGGCATTCGTGTTTGGGGTTGCAACACTGACTTGAAAGAAGCTGAAGAATCGCGACCGGAGTCTGTGCAGGTCATTGAAGACCTTGAAGCCTTTAAGATGTCATCTGAGTTCTATCCTCACGGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

735

Amino Acids

85.54

Weight (kDa)

5.32

Isoelectric Point (pI)

49.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 67 - 196 1.1e-34 Insulinase (Peptidase family M16)
Peptidase_M16_M PF16187 286 - 569 7.4e-77 Middle or third domain of peptidase_M16
PqqF-like_C_4 PF22456 589 - 647 1.1e-06 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 276, 948
Acc36I ACCTGC 1 cut(s) 2137
AccB1I GGYRCC 3 cut(s) 191, 434, 552
AccI GTMKAC 3 cut(s) 1255, 1964, 2008
AccII CGCG 1 cut(s) 2131
AciI CCGC 2 cut(s) 218, 1798
AclWI GGATC 4 cut(s) 129, 142, 1133, 1146
AcsI RAATTY 4 cut(s) 605, 910, 1555, 1906
AcuI CTGAAG 4 cut(s) 879, 988, 1511, 2142
AcyI GRCGYC 1 cut(s) 553
AfaI GTAC 8 cut(s) 65, 310, 504, 1286, 1300, 1386, 1673, 1778
AfiI CCNNNNNNNGG 4 cut(s) 357, 1082, 1771, 2070
AflII CTTAAG 1 cut(s) 1274
AjiI CACGTC 1 cut(s) 412
AjnI CCWGG 3 cut(s) 586, 834, 879
Alw21I GWGCWC 2 cut(s) 393, 2001
Alw26I GTCTC 4 cut(s) 48, 194, 564, 2068
AlwI GGATC 4 cut(s) 129, 142, 1133, 1146
AlwNI CAGNNNCTG 1 cut(s) 1609
AoxI GGCC 2 cut(s) 88, 1434
ApeKI GCWGC 9 cut(s) 9, 170, 173, 212, 215, 240, 874, 1603, 1795
ApoI RAATTY 4 cut(s) 605, 910, 1555, 1906
ArsI GACNNNNNNTTYG 2 cut(s) 2067, 2099
Asp700I GAANNNNTTC 1 cut(s) 990
AspLEI GCGC 3 cut(s) 277, 555, 949
AspS9I GGNCC 1 cut(s) 1761
AsuHPI GGTGA 6 cut(s) 459, 576, 584, 721, 798, 1912
AsuII TTCGAA 1 cut(s) 1333
AvaII GGWCC 1 cut(s) 1761
BamHI GGATCC 2 cut(s) 134, 1138
BanI GGYRCC 3 cut(s) 191, 434, 552
BanII GRGCYC 2 cut(s) 365, 2001
BbsI GAAGAC 2 cut(s) 1836, 2163
Bbv12I GWGCWC 2 cut(s) 393, 2001
BbvI GCAGC 8 cut(s) 182, 185, 224, 227, 252, 886, 1615, 1807
BccI CCATC 4 cut(s) 182, 1018, 1117, 1211
BciT130I CCWGG 3 cut(s) 588, 836, 881
BciVI GTATCC 1 cut(s) 1447
BcoDI GTCTC 4 cut(s) 48, 194, 564, 2068
BfaI CTAG 1 cut(s) 651
BfmI CTRYAG 2 cut(s) 510, 2009
BfoI RGCGCY 1 cut(s) 556
BfrI CTTAAG 1 cut(s) 1274
BfuAI ACCTGC 1 cut(s) 2137
BfuI GTATCC 1 cut(s) 1447
BlpI GCTNAGC 1 cut(s) 2000
Bme1390I CCNGG 3 cut(s) 588, 836, 881
Bme18I GGWCC 1 cut(s) 1761
BmgBI CACGTC 1 cut(s) 412
BmgT120I GGNCC 1 cut(s) 1761
BmiI GGNNCC 6 cut(s) 136, 193, 436, 554, 1140, 1763
BmrFI CCNGG 3 cut(s) 588, 836, 881
BmrI ACTGGG 2 cut(s) 147, 1759
BmsI GCATC 1 cut(s) 1174
BmuI ACTGGG 2 cut(s) 147, 1759
BoxI GACNNNNGTC 1 cut(s) 2137
BpiI GAAGAC 2 cut(s) 1836, 2163
BpmI CTGGAG 2 cut(s) 818, 1128
Bpu1102I GCTNAGC 1 cut(s) 2000
Bpu14I TTCGAA 1 cut(s) 1333
BpuEI CTTGAG 1 cut(s) 101
BsaHI GRCGYC 1 cut(s) 553
BsaI GGTCTC 1 cut(s) 564
BsaJI CCNNGG 5 cut(s) 21, 489, 879, 1076, 1313
BsaWI WCCGGW 2 cut(s) 319, 2134
BsaXI ACNNNNNCTCC 2 cut(s) 1228, 1258
Bsc4I CCNNNNNNNGG 4 cut(s) 357, 1082, 1771, 2070
Bse1I ACTGG 5 cut(s) 153, 1437, 1699, 1765, 1851
Bse3DI GCAATG 3 cut(s) 98, 228, 956
BseBI CCWGG 3 cut(s) 588, 836, 881
BseDI CCNNGG 5 cut(s) 21, 489, 879, 1076, 1313
BseGI GGATG 8 cut(s) 578, 889, 976, 1029, 1033, 1855, 1910, 2026
BseLI CCNNNNNNNGG 4 cut(s) 357, 1082, 1771, 2070
BseMI GCAATG 3 cut(s) 98, 228, 956
BseMII CTCAG 6 cut(s) 108, 210, 467, 1628, 2014, 2175
BseNI ACTGG 5 cut(s) 153, 1437, 1699, 1765, 1851
BseRI GAGGAG 2 cut(s) 10, 884
BseXI GCAGC 8 cut(s) 182, 185, 224, 227, 252, 886, 1615, 1807
BsgI GTGCAG 1 cut(s) 2165
Bsh1236I CGCG 1 cut(s) 2131
Bsh1285I CGRYCG 1 cut(s) 2135
BshFI GGCC 2 cut(s) 90, 1436
BshNI GGYRCC 3 cut(s) 191, 434, 552
BsiEI CGRYCG 1 cut(s) 2135
BsiHKAI GWGCWC 2 cut(s) 393, 2001
BsiSI CCGG 2 cut(s) 320, 2135
BslFI GGGAC 1 cut(s) 1747
BslI CCNNNNNNNGG 4 cut(s) 357, 1082, 1771, 2070
BsmAI GTCTC 4 cut(s) 48, 194, 564, 2068
BsmFI GGGAC 1 cut(s) 1747
BsmI GAATGC 1 cut(s) 2082
BsnI GGCC 2 cut(s) 90, 1436
Bso31I GGTCTC 1 cut(s) 564
Bsp119I TTCGAA 1 cut(s) 1333
Bsp1286I GDGCHC 3 cut(s) 365, 393, 2001
Bsp1407I TGTACA 1 cut(s) 63
Bsp143I GATC 4 cut(s) 134, 1138, 1294, 1573
Bsp1720I GCTNAGC 1 cut(s) 2000
Bsp19I CCATGG 1 cut(s) 489
Bsp68I TCGCGA 1 cut(s) 2131
BspACI CCGC 2 cut(s) 218, 1798
BspANI GGCC 2 cut(s) 90, 1436
BspCNI CTCAG 6 cut(s) 109, 209, 466, 1629, 2013, 2176
BspFNI CGCG 1 cut(s) 2131
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 6 cut(s) 136, 193, 436, 554, 1140, 1763
BspMI ACCTGC 1 cut(s) 2137
BspPI GGATC 4 cut(s) 129, 142, 1133, 1146
BspQI GCTCTTC 3 cut(s) 302, 381, 1989
BspT104I TTCGAA 1 cut(s) 1333
BspT107I GGYRCC 3 cut(s) 191, 434, 552
BspTI CTTAAG 1 cut(s) 1274
BspTNI GGTCTC 1 cut(s) 564
BsrDI GCAATG 3 cut(s) 98, 228, 956
BsrGI TGTACA 1 cut(s) 63
BsrI ACTGG 5 cut(s) 153, 1437, 1699, 1765, 1851
BssECI CCNNGG 5 cut(s) 21, 489, 879, 1076, 1313
BssMI GATC 4 cut(s) 134, 1138, 1294, 1573
BssNAI GTATAC 2 cut(s) 1256, 1965
BssNI GRCGYC 1 cut(s) 553
BssT1I CCWWGG 2 cut(s) 489, 1076
Bst1107I GTATAC 2 cut(s) 1256, 1965
Bst2UI CCWGG 3 cut(s) 588, 836, 881
Bst4CI ACNGT 5 cut(s) 63, 341, 507, 1384, 2201
Bst6I CTCTTC 4 cut(s) 302, 381, 1361, 1989
BstACI GRCGYC 1 cut(s) 553
BstAFI CTTAAG 1 cut(s) 1274
BstAUI TGTACA 1 cut(s) 63
BstBI TTCGAA 1 cut(s) 1333
BstC8I GCNNGC 2 cut(s) 273, 1073
BstDSI CCRYGG 2 cut(s) 489, 1313
BstEII GGTNACC 1 cut(s) 590
BstF5I GGATG 8 cut(s) 578, 889, 976, 1029, 1033, 1855, 1910, 2026
BstFNI CGCG 1 cut(s) 2131
BstH2I RGCGCY 1 cut(s) 556
BstHHI GCGC 3 cut(s) 277, 555, 949
BstKTI GATC 4 cut(s) 137, 1141, 1297, 1576
BstMAI GTCTC 4 cut(s) 48, 194, 564, 2068
BstMBI GATC 4 cut(s) 134, 1138, 1294, 1573
BstMCI CGRYCG 1 cut(s) 2135
BstMWI GCNNNNNNNGC 2 cut(s) 218, 1442
BstNI CCWGG 3 cut(s) 588, 836, 881
BstNSI RCATGY 2 cut(s) 938, 1709
BstPAI GACNNNNGTC 1 cut(s) 2137
BstPI GGTNACC 1 cut(s) 590
BstSCI CCNGG 3 cut(s) 586, 834, 879
BstSFI CTRYAG 2 cut(s) 510, 2009
BstUI CGCG 1 cut(s) 2131
BstV1I GCAGC 8 cut(s) 182, 185, 224, 227, 252, 886, 1615, 1807
BstV2I GAAGAC 2 cut(s) 1836, 2163
BstX2I RGATCY 2 cut(s) 134, 1138
BstXI CCANNNNNNTGG 2 cut(s) 816, 1758
BstYI RGATCY 2 cut(s) 134, 1138
BstZ17I GTATAC 2 cut(s) 1256, 1965
BsuI GTATCC 1 cut(s) 1447
BsuRI GGCC 2 cut(s) 90, 1436
BtgI CCRYGG 2 cut(s) 489, 1313
BtrI CACGTC 1 cut(s) 412
BtsCI GGATG 8 cut(s) 578, 889, 976, 1029, 1033, 1855, 1910, 2026
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 4 cut(s) 93, 160, 1073, 2103
BtuMI TCGCGA 1 cut(s) 2131
BveI ACCTGC 1 cut(s) 2137
Cac8I GCNNGC 2 cut(s) 273, 1073
CaiI CAGNNNCTG 1 cut(s) 1609
CciI TCATGA 1 cut(s) 109
CfoI GCGC 3 cut(s) 277, 555, 949
Cfr13I GGNCC 1 cut(s) 1761
CseI GACGC 3 cut(s) 785, 1595, 2057
CsiI ACCWGGT 1 cut(s) 586
Csp6I GTAC 8 cut(s) 64, 309, 503, 1285, 1299, 1385, 1672, 1777
CspCI CAANNNNNGTGG 2 cut(s) 1968, 2003
CviQI GTAC 8 cut(s) 64, 309, 503, 1285, 1299, 1385, 1672, 1777
DinI GGCGCC 1 cut(s) 554
DpnI GATC 4 cut(s) 136, 1140, 1296, 1575
DpnII GATC 4 cut(s) 134, 1138, 1294, 1573
DraI TTTAAA 2 cut(s) 1306, 1713
Eam1104I CTCTTC 4 cut(s) 302, 381, 1361, 1989
EarI CTCTTC 4 cut(s) 302, 381, 1361, 1989
Ecl136II GAGCTC 1 cut(s) 1999
Eco130I CCWWGG 2 cut(s) 489, 1076
Eco24I GRGCYC 2 cut(s) 365, 2001
Eco31I GGTCTC 1 cut(s) 564
Eco32I GATATC 1 cut(s) 1035
Eco47I GGWCC 1 cut(s) 1761
Eco53kI GAGCTC 1 cut(s) 1999
Eco57I CTGAAG 4 cut(s) 879, 988, 1511, 2142
Eco91I GGTNACC 1 cut(s) 590
EcoICRI GAGCTC 1 cut(s) 1999
EcoO65I GGTNACC 1 cut(s) 590
EcoRII CCWGG 3 cut(s) 586, 834, 879
EcoRV GATATC 1 cut(s) 1035
EcoT14I CCWWGG 2 cut(s) 489, 1076
EcoT38I GRGCYC 2 cut(s) 365, 2001
EgeI GGCGCC 1 cut(s) 554
EheI GGCGCC 1 cut(s) 554
ErhI CCWWGG 2 cut(s) 489, 1076
FalI AAGNNNNNCTT 2 cut(s) 2030, 2062
FaqI GGGAC 1 cut(s) 1747
FauNDI CATATG 1 cut(s) 291
FblI GTMKAC 3 cut(s) 1255, 1964, 2008
FokI GGATG 8 cut(s) 565, 896, 983, 1036, 1040, 1862, 1897, 2033
FriOI GRGCYC 2 cut(s) 365, 2001
FspBI CTAG 1 cut(s) 651
FspI TGCGCA 2 cut(s) 276, 948
GlaI GCGC 3 cut(s) 276, 554, 948
GsuI CTGGAG 2 cut(s) 818, 1128
HaeII RGCGCY 1 cut(s) 556
HaeIII GGCC 2 cut(s) 90, 1436
HapII CCGG 2 cut(s) 320, 2135
HgaI GACGC 3 cut(s) 785, 1595, 2057
HhaI GCGC 3 cut(s) 277, 555, 949
Hin1I GRCGYC 1 cut(s) 553
Hin6I GCGC 3 cut(s) 275, 553, 947
HinP1I GCGC 3 cut(s) 275, 553, 947
HincII GTYRAC 4 cut(s) 1380, 1533, 1807, 2029
HindII GTYRAC 4 cut(s) 1380, 1533, 1807, 2029
HindIII AAGCTT 3 cut(s) 77, 1289, 1514
HpaII CCGG 2 cut(s) 320, 2135
HphI GGTGA 6 cut(s) 459, 576, 584, 721, 798, 1912
Hpy166II GTNNAC 8 cut(s) 1088, 1256, 1380, 1533, 1807, 1965, 2009, 2029
Hpy8I GTNNAC 8 cut(s) 1088, 1256, 1380, 1533, 1807, 1965, 2009, 2029
Hpy99I CGWCG 2 cut(s) 1774, 2073
HpyAV CCTTC 6 cut(s) 202, 566, 1129, 1203, 1409, 2008
HpyCH4III ACNGT 5 cut(s) 63, 341, 507, 1384, 2201
HpyCH4IV ACGT 1 cut(s) 411
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 1442
HpySE526I ACGT 1 cut(s) 411
Hsp92I GRCGYC 1 cut(s) 553
HspAI GCGC 3 cut(s) 275, 553, 947
KasI GGCGCC 1 cut(s) 552
Kzo9I GATC 4 cut(s) 134, 1138, 1294, 1573
LguI GCTCTTC 3 cut(s) 302, 381, 1989
LmnI GCTCC 2 cut(s) 493, 871
Lsp1109I GCAGC 8 cut(s) 182, 185, 224, 227, 252, 886, 1615, 1807
LweI GCATC 1 cut(s) 1174
MabI ACCWGGT 1 cut(s) 586
MaeI CTAG 1 cut(s) 651
MaeII ACGT 1 cut(s) 411
MaeIII GTNAC 3 cut(s) 341, 590, 804
MalI GATC 4 cut(s) 136, 1140, 1296, 1575
MboI GATC 4 cut(s) 134, 1138, 1294, 1573
MflI RGATCY 2 cut(s) 134, 1138
MhlI GDGCHC 3 cut(s) 365, 393, 2001
Mly113I GGCGCC 1 cut(s) 553
MlyI GAGTC 3 cut(s) 728, 1648, 2147
MmeI TCCRAC 3 cut(s) 211, 1752, 2051
MroXI GAANNNNTTC 1 cut(s) 990
MslI CAYNNNNRTG 3 cut(s) 814, 1022, 1625
MspA1I CMGCKG 3 cut(s) 218, 1523, 1798
MspCI CTTAAG 1 cut(s) 1274
MspI CCGG 2 cut(s) 320, 2135
MspR9I CCNGG 3 cut(s) 588, 836, 881
Mva1269I GAATGC 1 cut(s) 2082
MvaI CCWGG 3 cut(s) 588, 836, 881
MvnI CGCG 1 cut(s) 2131
MwoI GCNNNNNNNGC 2 cut(s) 218, 1442
NarI GGCGCC 1 cut(s) 553
NcoI CCATGG 1 cut(s) 489
NdeI CATATG 1 cut(s) 291
NdeII GATC 4 cut(s) 134, 1138, 1294, 1573
NlaIV GGNNCC 6 cut(s) 136, 193, 436, 554, 1140, 1763
NmuCI GTSAC 2 cut(s) 590, 804
NruI TCGCGA 1 cut(s) 2131
NsbI TGCGCA 2 cut(s) 276, 948
NspI RCATGY 2 cut(s) 938, 1709
NspV TTCGAA 1 cut(s) 1333
PagI TCATGA 1 cut(s) 109
PciSI GCTCTTC 3 cut(s) 302, 381, 1989
PctI GAATGC 1 cut(s) 2082
PdmI GAANNNNTTC 1 cut(s) 990
PfeI GAWTC 7 cut(s) 515, 1335, 1630, 1680, 1856, 1942, 2126
PfoI TCCNGGA 1 cut(s) 834
PleI GAGTC 3 cut(s) 728, 1647, 2146
PluTI GGCGCC 1 cut(s) 556
PpsI GAGTC 3 cut(s) 728, 1647, 2146
PshAI GACNNNNGTC 1 cut(s) 2137
Psp124BI GAGCTC 1 cut(s) 2001
Psp6I CCWGG 3 cut(s) 586, 834, 879
PspEI GGTNACC 1 cut(s) 590
PspGI CCWGG 3 cut(s) 586, 834, 879
PspN4I GGNNCC 6 cut(s) 136, 193, 436, 554, 1140, 1763
PspPI GGNCC 1 cut(s) 1761
PstNI CAGNNNCTG 1 cut(s) 1609
PsuI RGATCY 2 cut(s) 134, 1138
PvuII CAGCTG 1 cut(s) 1523
RruI TCGCGA 1 cut(s) 2131
RsaI GTAC 8 cut(s) 65, 310, 504, 1286, 1300, 1386, 1673, 1778
RsaNI GTAC 8 cut(s) 64, 309, 503, 1285, 1299, 1385, 1672, 1777
RseI CAYNNNNRTG 3 cut(s) 814, 1022, 1625
SacI GAGCTC 1 cut(s) 2001
SapI GCTCTTC 3 cut(s) 302, 381, 1989
Sau3AI GATC 4 cut(s) 134, 1138, 1294, 1573
Sau96I GGNCC 1 cut(s) 1761
SchI GAGTC 3 cut(s) 728, 1648, 2147
ScrFI CCNGG 3 cut(s) 588, 836, 881
SduI GDGCHC 3 cut(s) 365, 393, 2001
SexAI ACCWGGT 1 cut(s) 586
SfaNI GCATC 1 cut(s) 1174
SfcI CTRYAG 2 cut(s) 510, 2009
SfoI GGCGCC 1 cut(s) 554
SfuI TTCGAA 1 cut(s) 1333
SinI GGWCC 1 cut(s) 1761
SmiMI CAYNNNNRTG 3 cut(s) 814, 1022, 1625
SmlI CTYRAG 2 cut(s) 80, 1274
SmoI CTYRAG 2 cut(s) 80, 1274
SsiI CCGC 2 cut(s) 218, 1798
SspDI GGCGCC 1 cut(s) 552
SspI AATATT 1 cut(s) 721
SspMI CTAG 1 cut(s) 651
SstI GAGCTC 1 cut(s) 2001
StyD4I CCNGG 3 cut(s) 586, 834, 879
StyI CCWWGG 2 cut(s) 489, 1076
TaaI ACNGT 5 cut(s) 63, 341, 507, 1384, 2201
TaiI ACGT 1 cut(s) 414
TaqI TCGA 3 cut(s) 568, 1333, 1395
TatI WGTACW 4 cut(s) 63, 308, 1384, 1776
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 7 cut(s) 515, 1335, 1630, 1680, 1856, 1942, 2126
TscAI CASTG 4 cut(s) 100, 160, 1073, 2110
TseFI GTSAC 2 cut(s) 590, 804
TseI GCWGC 9 cut(s) 9, 170, 173, 212, 215, 240, 874, 1603, 1795
Tsp45I GTSAC 2 cut(s) 590, 804
TspDTI ATGAA 9 cut(s) 98, 180, 289, 731, 1278, 1643, 1899, 1938, 1943
TspGWI ACGGA 1 cut(s) 1104
TspRI CASTG 4 cut(s) 100, 160, 1073, 2110
Vha464I CTTAAG 1 cut(s) 1274
VpaK11BI GGWCC 1 cut(s) 1761
XapI RAATTY 4 cut(s) 605, 910, 1555, 1906
XceI RCATGY 2 cut(s) 938, 1709
XmiI GTMKAC 3 cut(s) 1255, 1964, 2008
XmnI GAANNNNTTC 1 cut(s) 990
XspI CTAG 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.