RLG00000036350

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000183
Physical Location & Seq
Reverse (-)
79520088 .. 79528053
7966 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036350

Sequence Viewer

Length: 2499 bp
ATGGGTCGCTGCACTTTCTCCTCGGACGATATTGTAATAAAGTCTCCGAATGATAAGAAACTGTACAGGCTAATTAAGCTTCAGAATGGCCTCACTGCATTGCTCATTCATGACCCTGAGATATACCCACAAGGGTATAAGAGCCTTAAACCCAGTAAAGAAGATGAAGCAGCAGCTAAAGGTGGAGATGGTGCCTCAGAGACTAAGAAGGCAGCAGCAGCAATGTGCGTTGGAATGGGCAGCTTCTCTGACCCTATGGAAGCACAGGGGCTTGCGCACTTTCTTGAACATATGCTCTTCATGGGGAGTACAAAGTTTCCAGTTGAAAATGAGTATAACAGTTACTTGTCCGAGCATGGGGGCTCATCAAATGCTTACACAAAGGAAGAGCACACTTGCTACCATTTTGAAGTGAAACGAGAGTTTCTTAAAGAATCTCTCAACGTACTTGAGCAGTGGGTTCTGAAGTTGTTTGGAGACGTCAGAAAAGGTCCCCAAGTAAATCTTGAGTTCAAGGCAGAAGGTCCTATTTGGAACGTTGGAAAGCTTTACAGGCTAGAGGCTGTTAATGATGTTCCTATGCTCAACTTGGCATGGACACTTCCATGTCTTACTCAACACTATTTGAAGAGCCCAGAATATTATTTATGGCATCTCCTTGCGCATGAGAGCAGGGGAGGTTTGCAGTTCTACCTCAAAACTAGAGGGTGGGCAGTATCTCTAAGTGCTTGGGTGGAGAGCTATTCTGTGGCTTATGTCTTTGGCATGGATATTTACCTCACCGACTCTGGATTGGAGAAGATTTTTGAAATTATTGGGTTCGTGTATCAATACATTAAACTATTGCGTCAAGTGTCACCACAAAAATGGATATTTCAAGAACTCCAGGATATTCGGAACATACGCTTTAGATTTGTAGAGGAGCAGCCCCAGGATCACTATGCTTCAGAACTTGCAGATTTTCAGTGCGAGCCTTGGTTTGGGTCACATTATATTGAGGAAGATGTATCTCCGTTTTTGATGGATTTGTGGAAGGATCCTCCAGAGATTGATGTTTCGTTGCATCTCCGGGAAAAGAATGAGTTTATTCCTTCTGATTTTTCCATTCGTTCTGATGGTCTTGATACTACATATGCATCTTCTCCGAGATGTATACTTGATGAACCTTTGCTTAAGTTCTGGCACAAGCTTGATCGTACTTTTAAAGTTCCACGGGCAAATATATACTTTCGAATCAGTCTAAAGGGTGGATTTGATGACTTGAAGAGTTCTCTGTTGACTGTACTATATATTGACCTTCTTAGAGATAAGCTGAATGAGATTTTATATCAGGCCAGTTTTGCCCTGCTGGATACTTCTTTGTATGTGAATAATTACTATCTGGAACTGAAGCTGTGGGGCTTCAATGATAAGCTTCCAGCTGTATTGTCAACAATTCTGACAACAACCAAAAATTTCCTGCCAACGTATGATCGTTTCAAGGTTATTAAAGAAAATATGGAGCGTCTGATAAAGAACACTAATATGAAGCCTCTGAGTCATGCTGAATACTTGAAACTGCAAGTTTTGTACCAGAGATTCTACGATGTAGATGAGCAGTTGCATGTTTTAAATGGATTGTCTGTTTCTGATGTGAAGTCATTTATTCCAGAGATTTGGTCCCAGCTTTACATTGAAGGCCTTGGTCATGGAAATCTGTTAGAAGAAGAAGCAATTAGCCTTTTGAACATGGTGAAAACAATTTTTTCTGGACAACCACTTCCTACCGAACTGATGCTTAAGAACAATTGTATTTGTCTCGCTCCTGATGCTAACCTCATTAGAGATGTCAGTGTGAAGTATAAGACTGAAACAAACTCTGTGATTCAGCTATATTTTCAAATTGAGCGGGCAGTGGGGATAGAGTCCACCAGATTGAGAGCATTAATAGATCTTTTTGATGAAATTATAGACGAACCATTTTACGATCAACTCAGGATGAAGGAGCAGCTGGGATATGTTGTTCAGTGTGACTGGGAGGTGATATTTGGTGTTTTTGGCCTTTATTTCATAATTCAGTCGTCGGAGTACAACCCAATCGACTTGCAGCGGAGAGTTGACAACTTTATCAATGGTCTGGAAGACATTTTGCAAGGACTGGATGATGGTTCCTTTGAGAATTATAGAGATGGGCTAATGGCAAAGCTATTGGTGAAAAATTCATCCCTTAGACATGAAACCAATCGATTTTGGAATCAGATTATTCATAAACGGTATACATTTGACTATGCAAAGAGGGTGGCAGAAGAGCTCAGCAGTCTACAGAAGGAGGATGTTGTCAACTTTTACAAGACTTACTTGCACCAATCATCTCCCAAGCGTCGGAGACTTGCCATTCGTGTTTGGGGTTGCAACACTGACTTGGAAGAAGCTGCAGAATCACGATGGGAGTCTGTGCAGGTCATTGAAGACCTTGAAGCCTTTAAGATGTCATCTGAGTTCTATCCTAACGGTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

833

Amino Acids

96.73

Weight (kDa)

5.36

Isoelectric Point (pI)

46.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 67 - 156 2e-26 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 146 - 297 2.3e-10 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 319 - 535 2.6e-57 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 540 - 725 1.7e-13 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 645 - 744 1.7e-23 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 483
Acc16I TGCGCA 2 cut(s) 276, 663
Acc36I ACCTGC 1 cut(s) 2428
AccB1I GGYRCC 1 cut(s) 191
AccBSI CCGCTC 1 cut(s) 1888
AccI GTMKAC 3 cut(s) 1153, 2255, 2299
AciI CCGC 2 cut(s) 1888, 2089
AclI AACGTT 1 cut(s) 537
AclWI GGATC 3 cut(s) 942, 1031, 1044
AcsI RAATTY 2 cut(s) 1453, 2197
AcuI CTGAAG 4 cut(s) 65, 485, 930, 1409
AcyI GRCGYC 1 cut(s) 480
AfaI GTAC 7 cut(s) 65, 310, 447, 1198, 1284, 1571, 2069
AfiI CCNNNNNNNGG 3 cut(s) 357, 980, 2361
AflII CTTAAG 2 cut(s) 1172, 1778
AjnI CCWGG 2 cut(s) 885, 930
AloI GAACNNNNNNTCC 2 cut(s) 1986, 2018
Alw21I GWGCWC 2 cut(s) 393, 2292
Alw26I GTCTC 5 cut(s) 48, 194, 471, 1802, 2359
AlwI GGATC 3 cut(s) 942, 1031, 1044
AoxI GGCC 4 cut(s) 88, 1332, 1678, 2038
ApoI RAATTY 2 cut(s) 1453, 2197
ArsI GACNNNNNNTTYG 2 cut(s) 2358, 2390
AseI ATTAAT 1 cut(s) 1925
AspLEI GCGC 2 cut(s) 277, 664
AspS9I GGNCC 3 cut(s) 491, 524, 1659
AsuC2I CCSGG 1 cut(s) 1070
AsuHPI GGTGA 5 cut(s) 772, 849, 1744, 2032, 2203
AsuII TTCGAA 1 cut(s) 1231
AvaII GGWCC 3 cut(s) 491, 524, 1659
BamHI GGATCC 1 cut(s) 1036
BanI GGYRCC 1 cut(s) 191
BanII GRGCYC 3 cut(s) 365, 635, 2292
BbsI GAAGAC 2 cut(s) 2127, 2454
Bbv12I GWGCWC 2 cut(s) 393, 2292
BccI CCATC 6 cut(s) 182, 1015, 1109, 2138, 2162, 2418
BciT130I CCWGG 2 cut(s) 887, 932
BciVI GTATCC 1 cut(s) 1345
BcnI CCSGG 1 cut(s) 1070
BcoDI GTCTC 5 cut(s) 48, 194, 471, 1802, 2359
BfaI CTAG 2 cut(s) 557, 702
BfmI CTRYAG 2 cut(s) 2300, 2412
BfrI CTTAAG 2 cut(s) 1172, 1778
BfuAI ACCTGC 1 cut(s) 2428
BfuI GTATCC 1 cut(s) 1345
BglII AGATCT 1 cut(s) 1930
BlpI GCTNAGC 1 cut(s) 2291
Bme1390I CCNGG 3 cut(s) 887, 932, 1070
Bme18I GGWCC 3 cut(s) 491, 524, 1659
BmgT120I GGNCC 3 cut(s) 491, 524, 1659
BmiI GGNNCC 5 cut(s) 193, 493, 1038, 1661, 2149
BmrFI CCNGG 3 cut(s) 887, 932, 1070
BmrI ACTGGG 2 cut(s) 147, 2023
BmsI GCATC 5 cut(s) 661, 1072, 1145, 1764, 1798
BmuI ACTGGG 2 cut(s) 147, 2023
BpiI GAAGAC 2 cut(s) 2127, 2454
BpmI CTGGAG 2 cut(s) 869, 1026
Bpu1102I GCTNAGC 1 cut(s) 2291
Bpu14I TTCGAA 1 cut(s) 1231
BpuEI CTTGAG 2 cut(s) 470, 527
BpuMI CCSGG 1 cut(s) 1070
Bsa29I ATCGAT 1 cut(s) 2224
BsaHI GRCGYC 1 cut(s) 480
BsaJI CCNNGG 5 cut(s) 21, 930, 974, 1211, 1681
Bsc4I CCNNNNNNNGG 3 cut(s) 357, 980, 2361
Bse1I ACTGG 5 cut(s) 153, 320, 1335, 2018, 2142
Bse3DI GCAATG 2 cut(s) 98, 228
BseBI CCWGG 2 cut(s) 887, 932
BseCI ATCGAT 1 cut(s) 2224
BseDI CCNNGG 5 cut(s) 21, 930, 974, 1211, 1681
BseGI GGATG 4 cut(s) 1983, 2146, 2201, 2317
BseLI CCNNNNNNNGG 3 cut(s) 357, 980, 2361
BseMI GCAATG 2 cut(s) 98, 228
BseMII CTCAG 6 cut(s) 108, 210, 1526, 1987, 2305, 2466
BseNI ACTGG 5 cut(s) 153, 320, 1335, 2018, 2142
BseRI GAGGAG 2 cut(s) 10, 935
BseYI CCCAGC 2 cut(s) 1662, 1990
BsgI GTGCAG 1 cut(s) 2456
Bsh1285I CGRYCG 1 cut(s) 2494
BshFI GGCC 4 cut(s) 90, 1334, 1680, 2040
BshNI GGYRCC 1 cut(s) 191
BshVI ATCGAT 1 cut(s) 2224
BsiEI CGRYCG 1 cut(s) 2494
BsiHKAI GWGCWC 2 cut(s) 393, 2292
BsiSI CCGG 1 cut(s) 1069
BslFI GGGAC 2 cut(s) 477, 1645
BslI CCNNNNNNNGG 3 cut(s) 357, 980, 2361
BsmAI GTCTC 5 cut(s) 48, 194, 471, 1802, 2359
BsmBI CGTCTC 1 cut(s) 471
BsmFI GGGAC 2 cut(s) 477, 1645
BsnI GGCC 4 cut(s) 90, 1334, 1680, 2040
Bsp119I TTCGAA 1 cut(s) 1231
Bsp1286I GDGCHC 4 cut(s) 365, 393, 635, 2292
Bsp1407I TGTACA 1 cut(s) 63
Bsp143I GATC 6 cut(s) 934, 1036, 1192, 1471, 1930, 1966
Bsp1720I GCTNAGC 1 cut(s) 2291
BspACI CCGC 2 cut(s) 1888, 2089
BspANI GGCC 4 cut(s) 90, 1334, 1680, 2040
BspCNI CTCAG 6 cut(s) 109, 209, 1527, 1986, 2304, 2467
BspDI ATCGAT 1 cut(s) 2224
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 5 cut(s) 193, 493, 1038, 1661, 2149
BspMAI CTGCAG 1 cut(s) 2416
BspMI ACCTGC 1 cut(s) 2428
BspPI GGATC 3 cut(s) 942, 1031, 1044
BspQI GCTCTTC 4 cut(s) 302, 381, 623, 2280
BspT104I TTCGAA 1 cut(s) 1231
BspT107I GGYRCC 1 cut(s) 191
BspTI CTTAAG 2 cut(s) 1172, 1778
BsrBI CCGCTC 1 cut(s) 1888
BsrDI GCAATG 2 cut(s) 98, 228
BsrGI TGTACA 1 cut(s) 63
BsrI ACTGG 5 cut(s) 153, 320, 1335, 2018, 2142
BssECI CCNNGG 5 cut(s) 21, 930, 974, 1211, 1681
BssMI GATC 6 cut(s) 934, 1036, 1192, 1471, 1930, 1966
BssNAI GTATAC 2 cut(s) 1154, 2256
BssNI GRCGYC 1 cut(s) 480
BssT1I CCWWGG 2 cut(s) 974, 1681
Bst1107I GTATAC 2 cut(s) 1154, 2256
Bst2UI CCWGG 2 cut(s) 887, 932
Bst4CI ACNGT 5 cut(s) 63, 341, 1282, 2253, 2492
Bst6I CTCTTC 5 cut(s) 302, 381, 623, 1259, 2280
BstACI GRCGYC 1 cut(s) 480
BstAFI CTTAAG 2 cut(s) 1172, 1778
BstAUI TGTACA 1 cut(s) 63
BstBI TTCGAA 1 cut(s) 1231
BstC8I GCNNGC 3 cut(s) 273, 971, 1890
BstDSI CCRYGG 1 cut(s) 1211
BstF5I GGATG 4 cut(s) 1983, 2146, 2201, 2317
BstHHI GCGC 2 cut(s) 277, 664
BstKTI GATC 6 cut(s) 937, 1039, 1195, 1474, 1933, 1969
BstMAI GTCTC 5 cut(s) 48, 194, 471, 1802, 2359
BstMBI GATC 6 cut(s) 934, 1036, 1192, 1471, 1930, 1966
BstMCI CGRYCG 1 cut(s) 2494
BstMWI GCNNNNNNNGC 5 cut(s) 76, 218, 553, 1340, 1808
BstNI CCWGG 2 cut(s) 887, 932
BstNSI RCATGY 1 cut(s) 1607
BstSCI CCNGG 3 cut(s) 885, 930, 1068
BstSFI CTRYAG 2 cut(s) 2300, 2412
BstV2I GAAGAC 2 cut(s) 2127, 2454
BstX2I RGATCY 2 cut(s) 1036, 1930
BstXI CCANNNNNNTGG 2 cut(s) 867, 1656
BstYI RGATCY 2 cut(s) 1036, 1930
BstZ17I GTATAC 2 cut(s) 1154, 2256
Bsu15I ATCGAT 1 cut(s) 2224
BsuI GTATCC 1 cut(s) 1345
BsuRI GGCC 4 cut(s) 90, 1334, 1680, 2040
BsuTUI ATCGAT 1 cut(s) 2224
BtgI CCRYGG 1 cut(s) 1211
BtsCI GGATG 4 cut(s) 1983, 2146, 2201, 2317
BtsI GCAGTG 3 cut(s) 93, 461, 1899
BtsIMutI CAGTG 7 cut(s) 93, 461, 971, 1837, 1899, 2012, 2394
BveI ACCTGC 1 cut(s) 2428
Cac8I GCNNGC 3 cut(s) 273, 971, 1890
CciI TCATGA 1 cut(s) 109
CfoI GCGC 2 cut(s) 277, 664
Cfr13I GGNCC 3 cut(s) 491, 524, 1659
ClaI ATCGAT 1 cut(s) 2224
CseI GACGC 3 cut(s) 836, 1493, 2348
Csp6I GTAC 7 cut(s) 64, 309, 446, 1197, 1283, 1570, 2068
CspCI CAANNNNNGTGG 2 cut(s) 2259, 2294
CviQI GTAC 7 cut(s) 64, 309, 446, 1197, 1283, 1570, 2068
DpnI GATC 6 cut(s) 936, 1038, 1194, 1473, 1932, 1968
DpnII GATC 6 cut(s) 934, 1036, 1192, 1471, 1930, 1966
DraI TTTAAA 2 cut(s) 1204, 1611
Eam1104I CTCTTC 5 cut(s) 302, 381, 623, 1259, 2280
EarI CTCTTC 5 cut(s) 302, 381, 623, 1259, 2280
Ecl136II GAGCTC 1 cut(s) 2290
Eco130I CCWWGG 2 cut(s) 974, 1681
Eco147I AGGCCT 1 cut(s) 1680
Eco24I GRGCYC 3 cut(s) 365, 635, 2292
Eco47I GGWCC 3 cut(s) 491, 524, 1659
Eco53kI GAGCTC 1 cut(s) 2290
Eco57I CTGAAG 4 cut(s) 65, 485, 930, 1409
EcoICRI GAGCTC 1 cut(s) 2290
EcoO109I RGGNCCY 2 cut(s) 491, 524
EcoRII CCWGG 2 cut(s) 885, 930
EcoT14I CCWWGG 2 cut(s) 974, 1681
EcoT22I ATGCAT 1 cut(s) 1138
EcoT38I GRGCYC 3 cut(s) 365, 635, 2292
ErhI CCWWGG 2 cut(s) 974, 1681
Esp3I CGTCTC 1 cut(s) 471
FalI AAGNNNNNCTT 4 cut(s) 489, 521, 2321, 2353
FaqI GGGAC 2 cut(s) 477, 1645
FauI CCCGC 1 cut(s) 1881
FauNDI CATATG 2 cut(s) 291, 1132
FblI GTMKAC 3 cut(s) 1153, 2255, 2299
FokI GGATG 4 cut(s) 1990, 2153, 2188, 2324
FriOI GRGCYC 3 cut(s) 365, 635, 2292
FspBI CTAG 2 cut(s) 557, 702
FspI TGCGCA 2 cut(s) 276, 663
GlaI GCGC 2 cut(s) 276, 663
GsaI CCCAGC 2 cut(s) 1666, 1994
GsuI CTGGAG 2 cut(s) 869, 1026
HaeIII GGCC 4 cut(s) 90, 1334, 1680, 2040
HapII CCGG 1 cut(s) 1069
HgaI GACGC 3 cut(s) 836, 1493, 2348
HhaI GCGC 2 cut(s) 277, 664
Hin1I GRCGYC 1 cut(s) 480
Hin6I GCGC 2 cut(s) 275, 662
HinP1I GCGC 2 cut(s) 275, 662
HincII GTYRAC 4 cut(s) 1278, 1431, 2098, 2320
HindII GTYRAC 4 cut(s) 1278, 1431, 2098, 2320
HindIII AAGCTT 4 cut(s) 77, 545, 1187, 1412
HpaII CCGG 1 cut(s) 1069
HphI GGTGA 5 cut(s) 772, 849, 1744, 2032, 2203
Hpy166II GTNNAC 8 cut(s) 1154, 1278, 1431, 1908, 2098, 2256, 2300, 2320
Hpy8I GTNNAC 8 cut(s) 1154, 1278, 1431, 1908, 2098, 2256, 2300, 2320
Hpy99I CGWCG 2 cut(s) 2065, 2364
HpyAV CCTTC 8 cut(s) 202, 515, 1027, 1101, 1307, 1670, 1975, 2299
HpyCH4III ACNGT 5 cut(s) 63, 341, 1282, 2253, 2492
HpyCH4IV ACGT 4 cut(s) 444, 480, 537, 1466
HpyF10VI GCNNNNNNNGC 5 cut(s) 76, 218, 553, 1340, 1808
HpySE526I ACGT 4 cut(s) 444, 480, 537, 1466
Hsp92I GRCGYC 1 cut(s) 480
HspAI GCGC 2 cut(s) 275, 662
Kzo9I GATC 6 cut(s) 934, 1036, 1192, 1471, 1930, 1966
LguI GCTCTTC 4 cut(s) 302, 381, 623, 2280
LmnI GCTCC 4 cut(s) 922, 1501, 1807, 1984
LweI GCATC 5 cut(s) 661, 1072, 1145, 1764, 1798
MaeI CTAG 2 cut(s) 557, 702
MaeII ACGT 4 cut(s) 444, 480, 537, 1466
MaeIII GTNAC 4 cut(s) 341, 855, 984, 2009
MalI GATC 6 cut(s) 936, 1038, 1194, 1473, 1932, 1968
MbiI CCGCTC 1 cut(s) 1888
MboI GATC 6 cut(s) 934, 1036, 1192, 1471, 1930, 1966
MfeI CAATTG 1 cut(s) 1786
MflI RGATCY 2 cut(s) 1036, 1930
MhlI GDGCHC 4 cut(s) 365, 393, 635, 2292
MlyI GAGTC 4 cut(s) 779, 1546, 1913, 2438
MmeI TCCRAC 4 cut(s) 211, 520, 2043, 2342
Mph1103I ATGCAT 1 cut(s) 1138
MslI CAYNNNNRTG 3 cut(s) 604, 865, 1523
MspA1I CMGCKG 3 cut(s) 1421, 1990, 2089
MspCI CTTAAG 2 cut(s) 1172, 1778
MspI CCGG 1 cut(s) 1069
MspR9I CCNGG 3 cut(s) 887, 932, 1070
MunI CAATTG 1 cut(s) 1786
MvaI CCWGG 2 cut(s) 887, 932
MwoI GCNNNNNNNGC 5 cut(s) 76, 218, 553, 1340, 1808
NciI CCSGG 1 cut(s) 1070
NdeI CATATG 2 cut(s) 291, 1132
NdeII GATC 6 cut(s) 934, 1036, 1192, 1471, 1930, 1966
NlaIV GGNNCC 5 cut(s) 193, 493, 1038, 1661, 2149
NmuCI GTSAC 3 cut(s) 855, 984, 2009
NsbI TGCGCA 2 cut(s) 276, 663
NsiI ATGCAT 1 cut(s) 1138
NspI RCATGY 1 cut(s) 1607
NspV TTCGAA 1 cut(s) 1231
PagI TCATGA 1 cut(s) 109
PceI AGGCCT 1 cut(s) 1680
PciSI GCTCTTC 4 cut(s) 302, 381, 623, 2280
PfeI GAWTC 6 cut(s) 434, 1233, 1578, 1864, 2233, 2417
PfoI TCCNGGA 2 cut(s) 885, 1068
PleI GAGTC 4 cut(s) 779, 1545, 1912, 2437
PpsI GAGTC 4 cut(s) 779, 1545, 1912, 2437
PpuMI RGGWCCY 2 cut(s) 491, 524
PshBI ATTAAT 1 cut(s) 1925
Psp124BI GAGCTC 1 cut(s) 2292
Psp1406I AACGTT 1 cut(s) 537
Psp5II RGGWCCY 2 cut(s) 491, 524
Psp6I CCWGG 2 cut(s) 885, 930
PspFI CCCAGC 2 cut(s) 1662, 1990
PspGI CCWGG 2 cut(s) 885, 930
PspN4I GGNNCC 5 cut(s) 193, 493, 1038, 1661, 2149
PspPI GGNCC 3 cut(s) 491, 524, 1659
PspPPI RGGWCCY 2 cut(s) 491, 524
PstI CTGCAG 1 cut(s) 2416
PsuI RGATCY 2 cut(s) 1036, 1930
PvuII CAGCTG 2 cut(s) 1421, 1990
RsaI GTAC 7 cut(s) 65, 310, 447, 1198, 1284, 1571, 2069
RsaNI GTAC 7 cut(s) 64, 309, 446, 1197, 1283, 1570, 2068
RseI CAYNNNNRTG 3 cut(s) 604, 865, 1523
SacI GAGCTC 1 cut(s) 2292
SapI GCTCTTC 4 cut(s) 302, 381, 623, 2280
Sau3AI GATC 6 cut(s) 934, 1036, 1192, 1471, 1930, 1966
Sau96I GGNCC 3 cut(s) 491, 524, 1659
SchI GAGTC 4 cut(s) 779, 1546, 1913, 2438
ScrFI CCNGG 3 cut(s) 887, 932, 1070
SduI GDGCHC 4 cut(s) 365, 393, 635, 2292
SfaNI GCATC 5 cut(s) 661, 1072, 1145, 1764, 1798
SfcI CTRYAG 2 cut(s) 2300, 2412
SfuI TTCGAA 1 cut(s) 1231
SinI GGWCC 3 cut(s) 491, 524, 1659
SmiMI CAYNNNNRTG 3 cut(s) 604, 865, 1523
SmlI CTYRAG 4 cut(s) 449, 506, 1172, 1778
SmoI CTYRAG 4 cut(s) 449, 506, 1172, 1778
SseBI AGGCCT 1 cut(s) 1680
SsiI CCGC 2 cut(s) 1888, 2089
SspI AATATT 1 cut(s) 641
SspMI CTAG 2 cut(s) 557, 702
SstI GAGCTC 1 cut(s) 2292
StuI AGGCCT 1 cut(s) 1680
StyD4I CCNGG 3 cut(s) 885, 930, 1068
StyI CCWWGG 2 cut(s) 974, 1681
TaaI ACNGT 5 cut(s) 63, 341, 1282, 2253, 2492
TaiI ACGT 4 cut(s) 447, 483, 540, 1469
TaqI TCGA 3 cut(s) 1231, 2079, 2224
TatI WGTACW 4 cut(s) 63, 308, 1282, 2067
TfiI GAWTC 6 cut(s) 434, 1233, 1578, 1864, 2233, 2417
TscAI CASTG 7 cut(s) 100, 461, 971, 1837, 1899, 2012, 2401
TseFI GTSAC 3 cut(s) 855, 984, 2009
Tsp45I GTSAC 3 cut(s) 855, 984, 2009
TspGWI ACGGA 1 cut(s) 1002
TspRI CASTG 7 cut(s) 100, 461, 971, 1837, 1899, 2012, 2401
Vha464I CTTAAG 2 cut(s) 1172, 1778
VpaK11BI GGWCC 3 cut(s) 491, 524, 1659
VspI ATTAAT 1 cut(s) 1925
XapI RAATTY 2 cut(s) 1453, 2197
XceI RCATGY 1 cut(s) 1607
XmiI GTMKAC 3 cut(s) 1153, 2255, 2299
XspI CTAG 2 cut(s) 557, 702
ZraI GACGTC 1 cut(s) 481
Zsp2I ATGCAT 1 cut(s) 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.