Rw5G044370

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000183
Physical Location & Seq
Reverse (-)
78063160 .. 78071336
8177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G044370.1

Sequence Viewer

Length: 3063 bp
ATGGGTATGGGTGTGACGGGTTGCTGCACTTTCTCGTCGGACGACATTGTAATAAAGTCTCCTAATGACAAACGACTCTACAGAGTGATTAAGCTTGAGAATGGCCTTACTGCATTGCTCATTCACGATCCTCAGGTCTACCCACAAGGACCACCCCGCCACTCCTCCAACCTTGAACAAGAAGAAGAAGAAGATTGTGAAGCTGTAGAAGAAGAAAAGGAAGAGGAAGGTGAAGTTAAAAGGAAGGAAGAGGGAGGTGCTTCTGAGACTAAGAAGGCAGCAGCAGCAATGTGTGTCGGAATAGGCAGCTTCTCTGACCCTCTCGAGGCTCAGGGGCTTGCACATTTTCTAGAACACATGCTGTTCATGGGGAGTACAAAGTTCCCAGATGAAAATGAGTATGCTAGTTACTTGTCCAAGCATGGAGGGTCGTCAAATGCATATACAGGAGCAGAACATACTTGCTACTACTTTAATGTGAAACAAGAATTTCTTATGGGTGCCTTGAAAAGATTTTCTCAGTTCTTTGTTTCACCTCTAATGAAAAGTGAAGCCATGGAGAGAGAGGTACAGGCTATAGATTCAGAGTTTAAAACGGTTCTGCAAAACGATTTCTCCCGCCTTCGACAACTTCAGGGCCATACATCCTCACCTGGTCACCCATTTAATAAATTCGCTTGGGGAAATAAGAAGAGCTTGGATGATGCAAAGGAAAAAGGGATAAACTTGCAGGAACGAATACTAAAATTGTACAGAGACTATTACCACGGTGGATTAATGAAGCTAGTTGTCATTGGTGGAGAATCTCTTGATATACTTGAGCACTGGGTTTTGGAATTGTTTGGAGATGTCAAAAAAGGTCCCCAAGTAAATCTGGAGTTCAAGGCAGAAGGTCCTATTTGGAAAGCTGGAAAACTTTACAGGCTAGAGGCTGTTGATGATGTTCATATACTCCACCTAGCATGGACACTTCCATGTCTTCAAGAACACTATTTGAAGAAACCAGAAGATTACTTGAGTCATCTGCTTGGGCATGAGGGCAGGGGAAGTTTGCATTTCTACCTGAAAGCTAGAGGGTGGGTAACATCTTTAGATACTTGTTTGAGCGGGATGGACTGCTCTTCTGTGGCTTATATCTTTTGCATGGTCATATACCTCACTGACTCTGGATTGGAGAAGATTTTTGAAATAATTGGGTTCGTGTATCAATACATTGAGTTATTGCGTCAAATGTTGCCACAAGAATGGATATTTCGGGAACTTCAGGATATTGGGAACATGGACTTTAGATTTGCAGAGGAGCAGGATCAGGATGATTATGCTTCACAACTTGCAGAAAATTTACTATATTATGCAACAGAGCATGTTATTTATGGGGACTATGTGAACGAGAGTTGGGACCAGGAATTGATAGAATATGTTCTTGGTTTCTTCAGACCAGAAAACATGAGGATTGATGTGGTATCAAAGTCCTTGTTTAAGTCAGAAGATTTCCAGTGTGAGCCTTGGTTTGGATCACATTATACCGAGGAAGATGTATCTCCATCTTTAATAAATTTATGGAAGAATCCTCAAGAAATTAGTGTTTCATTGCATCTGCCAGCAAAGAATGATTTCATTCCTCGTGATTTTTCCATCCGTTCTGATGTTTTGTGCATTGATACTGCAACGACATCTTGTCCGAGATGTATACTTGATGAACCATTGATGCAGTTTTGGTACAAGCTGGACAATACATTTAAACTTCCACATGTAAATACATATTTCCGCATCAATCTGAAGGGTGCATGTGATGATGTGAAGAGTTGTGTTTTGACTAGCTTATACCTTGACCTTCTCACAGATCAGCTGAATGAGATCCTCTATGAGGCCTGTGTTGCCAGCCTGGGAACTTCTTTTTCTCTGTCTCTGGACAAACTGCAGTTAGAGGTATACGGTTTCAATGATAAGCTTCCAGCTCTGTTGTCAAAAATTTTGGAAACAGTAAAAAGTTTCTTGCCAACTGATGATCGTTTTGAGGTTTTTAAAGAAGACATGGAGCGAGCATATACGAACGCCAATATGGATCCTTGGAGTTACTCAACATACTCGAGAGATCAAGTTCTGTTGAAGAAATTCTATACCGTAGATGAGCAGTCGCATGTTTTGAAGGGATTGTCTGTTTCTGATCTGAAGTCTTTCATTCCTGAGATTTTTTCCCAGCTATACATTGAGGGCCTTTTGCATGGCAACTTGTCAGAAGAAGAAGCTATTAGTCTTGCAAAATTATTTCAAACAAATTTTACTGTACCACCACTTCCTAGCGAGTTGGTGTATAGAGACAGTTGTATCTGTCTTCCTCCAAATGCTAACCTCATTAGAGATGCTATTGTGAAGAACAAGTCAGAAACAAACTCTGTGACTGAGCTGTATTTTCAAATTGAGCAAGCAGTGAAGATTGAGTCCATCAGACTAAAAGTATTGATTGATCTTTTTCATGAAATTGTACAGGAACCACTTTTTAATCAACTAAGGACAAAGGAGCAGCTTGGGTATGTTGTTCTCTGTGGCCAGAATCATACATGCAATGTTTTTGGCTTCTATTTCTGTGTTCAGTCGTCCGAGTACAACCCGATCCACCTTCAAGGCAGACTGGACAACTTTATCGATGGTCTGGAAGAGTTGTTGGAAGGACTGGACGATGATTCCTTTGAGAATTATAAAGGTGGACTAATGGCAAAGATTCTGGAGAAAGATGCATCCCTCACATGTGAAACCAATCGATTGTGGACTCAGATTCTTGCTAAATGGTACAAGTTTGACTATTCGAAAAAGGCTGCAGAACAATTCAGAAGCATTCAGAAGGAGGATGTTACCAAATTTTACAAGACCTATTTGCAACAATCATCTCCAAAGCGTCGAAGACTTGCAACTCGTGTTTGGGGTTGCAACACAGACTTGAAAGAAGCTGAAGAAGCGCGACCGGAGTCTGTGCAAGTCATTGAAGACCTTGCAGCCTTTAAGATGTCGTCCAAGTTTTATGATCGCAAAAGATGTCAAAAAGTGACTCCTCTAAATAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

1020

Amino Acids

117.33

Weight (kDa)

5.04

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 89 - 211 7.7e-33 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 245 - 422 1.3e-17 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 429 - 712 3.5e-84 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 719 - 902 3.3e-18 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 824 - 923 8e-24 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2700
AccB1I GGYRCC 1 cut(s) 500
AccBSI CCGCTC 1 cut(s) 1107
AccI GTMKAC 3 cut(s) 138, 1690, 1932
AccII CGCG 1 cut(s) 2959
AciI CCGC 4 cut(s) 157, 619, 1107, 1768
AclWI GGATC 7 cut(s) 122, 1314, 1522, 1852, 2060, 2073, 2608
AcoI YGGCCR 1 cut(s) 2548
AcsI RAATTY 8 cut(s) 488, 671, 1339, 1555, 1971, 2114, 2278, 2858
AcuI CTGAAG 6 cut(s) 617, 1247, 1417, 1799, 2192, 2970
AfaI GTAC 8 cut(s) 376, 570, 752, 1721, 2289, 2487, 2606, 2792
AfiI CCNNNNNNNGG 3 cut(s) 325, 1511, 1867
AflIII ACRYGT 2 cut(s) 1750, 2747
AhdI GACNNNNNGTC 1 cut(s) 1677
AjnI CCWGG 3 cut(s) 652, 1401, 1884
AjuI GAANNNNNNNTTGG 2 cut(s) 1407, 1439
Alw21I GWGCWC 1 cut(s) 825
Alw26I GTCTC 5 cut(s) 63, 260, 750, 1911, 2313
AlwI GGATC 7 cut(s) 122, 1314, 1522, 1852, 2060, 2073, 2608
Ama87I CYCGRG 2 cut(s) 323, 2089
AoxI GGCC 5 cut(s) 103, 637, 1869, 2215, 2548
ApeKI GCWGC 8 cut(s) 24, 278, 281, 284, 306, 2524, 2816, 2993
ApoI RAATTY 8 cut(s) 488, 671, 1339, 1555, 1971, 2114, 2278, 2858
ArsI GACNNNNNNTTYG 2 cut(s) 1274, 1306
AseI ATTAAT 1 cut(s) 776
Asp700I GAANNNNTTC 4 cut(s) 1419, 1613, 2114, 2177
AspLEI GCGC 1 cut(s) 2959
AspS9I GGNCC 6 cut(s) 149, 637, 860, 893, 1399, 2215
AsuHPI GGTGA 4 cut(s) 242, 525, 642, 650
AsuII TTCGAA 1 cut(s) 2807
AvaI CYCGRG 2 cut(s) 323, 2089
AvaII GGWCC 4 cut(s) 149, 860, 893, 1399
AxyI CCTNAGG 1 cut(s) 132
BaeI ACNNNNGTAYC 2 cut(s) 2311, 2344
BalI TGGCCA 1 cut(s) 2550
BamHI GGATCC 1 cut(s) 2065
BanI GGYRCC 1 cut(s) 500
BarI GAAGNNNNNNTAC 2 cut(s) 2280, 2312
BauI CACGAG 2 cut(s) 1623, 2913
BbsI GAAGAC 5 cut(s) 971, 2037, 2327, 2908, 2991
Bbv12I GWGCWC 1 cut(s) 825
BbvI GCAGC 8 cut(s) 11, 290, 293, 296, 318, 2536, 2803, 3005
BccI CCATC 5 cut(s) 1105, 1552, 1643, 2453, 2642
BciT130I CCWGG 3 cut(s) 654, 1403, 1886
BcoDI GTCTC 5 cut(s) 63, 260, 750, 1911, 2313
BfaI CTAG 8 cut(s) 350, 405, 785, 926, 959, 1071, 1818, 2301
BfmI CTRYAG 5 cut(s) 79, 204, 576, 1919, 2817
BisI GCNGC 8 cut(s) 25, 279, 282, 285, 307, 2525, 2817, 2994
BlsI GCNGC 8 cut(s) 26, 280, 283, 286, 308, 2526, 2818, 2995
Bme1390I CCNGG 3 cut(s) 654, 1403, 1886
Bme18I GGWCC 4 cut(s) 149, 860, 893, 1399
BmeRI GACNNNNNGTC 1 cut(s) 1677
BmeT110I CYCGRG 2 cut(s) 323, 2089
BmgT120I GGNCC 6 cut(s) 149, 637, 860, 893, 1399, 2215
BmiI GGNNCC 5 cut(s) 502, 862, 1400, 2067, 2493
BmrFI CCNGG 3 cut(s) 654, 1403, 1886
BmrI ACTGGG 1 cut(s) 835
BmsI GCATC 7 cut(s) 694, 1603, 1698, 1779, 2353, 2725, 2747
BmuI ACTGGG 1 cut(s) 835
BoxI GACNNNNGTC 1 cut(s) 2965
BpiI GAAGAC 5 cut(s) 971, 2037, 2327, 2908, 2991
BpmI CTGGAG 2 cut(s) 896, 2747
Bpu10I CCTNAGC 1 cut(s) 330
Bpu14I TTCGAA 1 cut(s) 2807
BpuEI CTTGAG 4 cut(s) 116, 839, 1036, 1557
Bsa29I ATCGAT 2 cut(s) 2646, 2761
BsaJI CCNNGG 6 cut(s) 555, 766, 1505, 1527, 1885, 2069
BsaWI WCCGGW 1 cut(s) 2962
BsaXI ACNNNNNCTCC 2 cut(s) 599, 629
Bsc4I CCNNNNNNNGG 3 cut(s) 325, 1511, 1867
Bse1I ACTGG 4 cut(s) 830, 1495, 2637, 2679
Bse21I CCTNAGG 1 cut(s) 132
Bse3DI GCAATG 4 cut(s) 113, 294, 1589, 2572
BseBI CCWGG 3 cut(s) 654, 1403, 1886
BseCI ATCGAT 2 cut(s) 2646, 2761
BseDI CCNNGG 6 cut(s) 555, 766, 1505, 1527, 1885, 2069
BseGI GGATG 7 cut(s) 644, 706, 1116, 1318, 1635, 2738, 2854
BseLI CCNNNNNNNGG 3 cut(s) 325, 1511, 1867
BseMI GCAATG 4 cut(s) 113, 294, 1589, 2572
BseMII CTCAG 7 cut(s) 146, 255, 344, 533, 2178, 2394, 2786
BseNI ACTGG 4 cut(s) 830, 1495, 2637, 2679
BseRI GAGGAG 3 cut(s) 154, 1313, 3039
BseXI GCAGC 8 cut(s) 11, 290, 293, 296, 318, 2536, 2803, 3005
BseYI CCCAGC 1 cut(s) 2199
BsgI GTGCAG 1 cut(s) 10
Bsh1236I CGCG 1 cut(s) 2959
Bsh1285I CGRYCG 1 cut(s) 2963
BshFI GGCC 5 cut(s) 105, 639, 1871, 2217, 2550
BshNI GGYRCC 1 cut(s) 500
BshVI ATCGAT 2 cut(s) 2646, 2761
BsiEI CGRYCG 1 cut(s) 2963
BsiHKAI GWGCWC 1 cut(s) 825
BsiHKCI CYCGRG 2 cut(s) 323, 2089
BsiSI CCGG 1 cut(s) 2963
BslFI GGGAC 3 cut(s) 846, 1391, 1412
BslI CCNNNNNNNGG 3 cut(s) 325, 1511, 1867
BsmAI GTCTC 5 cut(s) 63, 260, 750, 1911, 2313
BsmFI GGGAC 3 cut(s) 846, 1391, 1412
BsmI GAATGC 1 cut(s) 2835
BsnI GGCC 5 cut(s) 105, 639, 1871, 2217, 2550
BsoBI CYCGRG 2 cut(s) 323, 2089
Bsp119I TTCGAA 1 cut(s) 2807
Bsp1286I GDGCHC 1 cut(s) 825
Bsp1407I TGTACA 2 cut(s) 750, 2485
Bsp19I CCATGG 1 cut(s) 555
BspACI CCGC 4 cut(s) 157, 619, 1107, 1768
BspANI GGCC 5 cut(s) 105, 639, 1871, 2217, 2550
BspCNI CTCAG 7 cut(s) 145, 256, 343, 532, 2179, 2395, 2785
BspDI ATCGAT 2 cut(s) 2646, 2761
BspFNI CGCG 1 cut(s) 2959
BspHI TCATGA 1 cut(s) 2476
BspLI GGNNCC 5 cut(s) 502, 862, 1400, 2067, 2493
BspMAI CTGCAG 2 cut(s) 1923, 2821
BspPI GGATC 7 cut(s) 122, 1314, 1522, 1852, 2060, 2073, 2608
BspQI GCTCTTC 2 cut(s) 686, 1126
BspT104I TTCGAA 1 cut(s) 2807
BspT107I GGYRCC 1 cut(s) 500
BsrBI CCGCTC 1 cut(s) 1107
BsrDI GCAATG 4 cut(s) 113, 294, 1589, 2572
BsrGI TGTACA 2 cut(s) 750, 2485
BsrI ACTGG 4 cut(s) 830, 1495, 2637, 2679
BssECI CCNNGG 6 cut(s) 555, 766, 1505, 1527, 1885, 2069
BssNAI GTATAC 2 cut(s) 1691, 1933
BssSI CACGAG 2 cut(s) 1623, 2913
BssT1I CCWWGG 3 cut(s) 555, 1505, 2069
Bst1107I GTATAC 2 cut(s) 1691, 1933
Bst2BI CACGAG 2 cut(s) 1623, 2913
Bst2UI CCWGG 3 cut(s) 654, 1403, 1886
Bst4CI ACNGT 7 cut(s) 598, 770, 1937, 1984, 2125, 2287, 2324
Bst6I CTCTTC 6 cut(s) 216, 243, 686, 1126, 1796, 2652
BstAUI TGTACA 2 cut(s) 750, 2485
BstBI TTCGAA 1 cut(s) 2807
BstC8I GCNNGC 5 cut(s) 339, 1602, 1882, 2043, 2427
BstDEI CTNAG 9 cut(s) 132, 264, 270, 330, 519, 2187, 2403, 2510, 2772
BstDSI CCRYGG 2 cut(s) 555, 766
BstEII GGTNACC 1 cut(s) 656
BstENI CCTNNNNNAGG 1 cut(s) 1865
BstF5I GGATG 7 cut(s) 644, 706, 1116, 1318, 1635, 2738, 2854
BstFNI CGCG 1 cut(s) 2959
BstHHI GCGC 1 cut(s) 2959
BstMAI GTCTC 5 cut(s) 63, 260, 750, 1911, 2313
BstMCI CGRYCG 1 cut(s) 2963
BstMWI GCNNNNNNNGC 3 cut(s) 284, 1877, 2954
BstNI CCWGG 3 cut(s) 654, 1403, 1886
BstNSI RCATGY 7 cut(s) 361, 1367, 1754, 1791, 2144, 2565, 2751
BstPAI GACNNNNGTC 1 cut(s) 2965
BstPI GGTNACC 1 cut(s) 656
BstSCI CCNGG 3 cut(s) 652, 1401, 1884
BstSFI CTRYAG 5 cut(s) 79, 204, 576, 1919, 2817
BstUI CGCG 1 cut(s) 2959
BstV1I GCAGC 8 cut(s) 11, 290, 293, 296, 318, 2536, 2803, 3005
BstV2I GAAGAC 5 cut(s) 971, 2037, 2327, 2908, 2991
BstX2I RGATCY 2 cut(s) 1857, 2065
BstXI CCANNNNNNTGG 1 cut(s) 1245
BstYI RGATCY 2 cut(s) 1857, 2065
BstZ17I GTATAC 2 cut(s) 1691, 1933
Bsu15I ATCGAT 2 cut(s) 2646, 2761
Bsu36I CCTNAGG 1 cut(s) 132
BsuRI GGCC 5 cut(s) 105, 639, 1871, 2217, 2550
BsuTUI ATCGAT 2 cut(s) 2646, 2761
BtgI CCRYGG 2 cut(s) 555, 766
BtsCI GGATG 7 cut(s) 644, 706, 1116, 1318, 1635, 2738, 2854
BtsI GCAGTG 1 cut(s) 2436
BtsIMutI CAGTG 4 cut(s) 823, 1158, 1502, 2436
Cac8I GCNNGC 5 cut(s) 339, 1602, 1882, 2043, 2427
CciI TCATGA 1 cut(s) 2476
CfoI GCGC 1 cut(s) 2959
Cfr13I GGNCC 6 cut(s) 149, 637, 860, 893, 1399, 2215
ClaI ATCGAT 2 cut(s) 2646, 2761
CseI GACGC 2 cut(s) 1214, 2885
CsiI ACCWGGT 1 cut(s) 652
Csp6I GTAC 8 cut(s) 375, 569, 751, 1720, 2288, 2486, 2605, 2791
CviQI GTAC 8 cut(s) 375, 569, 751, 1720, 2288, 2486, 2605, 2791
DdeI CTNAG 9 cut(s) 132, 264, 270, 330, 519, 2187, 2403, 2510, 2772
DraI TTTAAA 3 cut(s) 592, 1741, 2026
DriI GACNNNNNGTC 1 cut(s) 1677
EaeI YGGCCR 1 cut(s) 2548
Eam1104I CTCTTC 6 cut(s) 216, 243, 686, 1126, 1796, 2652
Eam1105I GACNNNNNGTC 1 cut(s) 1677
EarI CTCTTC 6 cut(s) 216, 243, 686, 1126, 1796, 2652
Eco130I CCWWGG 3 cut(s) 555, 1505, 2069
Eco147I AGGCCT 1 cut(s) 1871
Eco47I GGWCC 4 cut(s) 149, 860, 893, 1399
Eco57I CTGAAG 6 cut(s) 617, 1247, 1417, 1799, 2192, 2970
Eco81I CCTNAGG 1 cut(s) 132
Eco88I CYCGRG 2 cut(s) 323, 2089
Eco91I GGTNACC 1 cut(s) 656
EcoNI CCTNNNNNAGG 1 cut(s) 1865
EcoO109I RGGNCCY 3 cut(s) 860, 893, 2215
EcoO65I GGTNACC 1 cut(s) 656
EcoRII CCWGG 3 cut(s) 652, 1401, 1884
EcoT14I CCWWGG 3 cut(s) 555, 1505, 2069
EcoT22I ATGCAT 2 cut(s) 442, 2740
ErhI CCWWGG 3 cut(s) 555, 1505, 2069
FalI AAGNNNNNCTT 4 cut(s) 477, 509, 680, 712
FaqI GGGAC 3 cut(s) 846, 1391, 1412
FauI CCCGC 3 cut(s) 164, 626, 1100
FblI GTMKAC 3 cut(s) 138, 1690, 1932
Fnu4HI GCNGC 8 cut(s) 25, 279, 282, 285, 307, 2525, 2817, 2994
FokI GGATG 7 cut(s) 631, 713, 1123, 1325, 1622, 2725, 2861
Fsp4HI GCNGC 8 cut(s) 25, 279, 282, 285, 307, 2525, 2817, 2994
FspBI CTAG 8 cut(s) 350, 405, 785, 926, 959, 1071, 1818, 2301
GlaI GCGC 1 cut(s) 2958
GluI GCNGC 8 cut(s) 25, 279, 282, 285, 307, 2525, 2817, 2994
GsaI CCCAGC 1 cut(s) 2203
GsuI CTGGAG 2 cut(s) 896, 2747
HaeIII GGCC 5 cut(s) 105, 639, 1871, 2217, 2550
HapII CCGG 1 cut(s) 2963
HgaI GACGC 2 cut(s) 1214, 2885
HhaI GCGC 1 cut(s) 2959
Hin6I GCGC 1 cut(s) 2957
HinP1I GCGC 1 cut(s) 2957
HindIII AAGCTT 2 cut(s) 92, 1949
HpaII CCGG 1 cut(s) 2963
HphI GGTGA 4 cut(s) 242, 525, 642, 650
Hpy166II GTNNAC 6 cut(s) 139, 1387, 1691, 1933, 2708, 2769
Hpy8I GTNNAC 6 cut(s) 139, 1387, 1691, 1933, 2708, 2769
Hpy99I CGWCG 2 cut(s) 40, 2901
HpyCH4III ACNGT 7 cut(s) 598, 770, 1937, 1984, 2125, 2287, 2324
HpyF10VI GCNNNNNNNGC 3 cut(s) 284, 1877, 2954
HpyF3I CTNAG 9 cut(s) 132, 264, 270, 330, 519, 2187, 2403, 2510, 2772
HspAI GCGC 1 cut(s) 2957
LguI GCTCTTC 2 cut(s) 686, 1126
LmnI GCTCC 4 cut(s) 449, 1300, 2038, 2521
Lsp1109I GCAGC 8 cut(s) 11, 290, 293, 296, 318, 2536, 2803, 3005
LweI GCATC 7 cut(s) 694, 1603, 1698, 1779, 2353, 2725, 2747
MabI ACCWGGT 1 cut(s) 652
MaeI CTAG 8 cut(s) 350, 405, 785, 926, 959, 1071, 1818, 2301
MaeIII GTNAC 8 cut(s) 13, 407, 656, 1081, 2075, 2398, 2851, 3043
MbiI CCGCTC 1 cut(s) 1107
MflI RGATCY 2 cut(s) 1857, 2065
MhlI GDGCHC 1 cut(s) 825
MlsI TGGCCA 1 cut(s) 2550
MluNI TGGCCA 1 cut(s) 2550
MlyI GAGTC 7 cut(s) 69, 1027, 1157, 2450, 2764, 2975, 3040
MmeI TCCRAC 4 cut(s) 18, 192, 277, 2646
Mox20I TGGCCA 1 cut(s) 2550
Mph1103I ATGCAT 2 cut(s) 442, 2740
MroXI GAANNNNTTC 4 cut(s) 1419, 1613, 2114, 2177
MscI TGGCCA 1 cut(s) 2550
MslI CAYNNNNRTG 2 cut(s) 973, 1243
Msp20I TGGCCA 1 cut(s) 2550
MspA1I CMGCKG 1 cut(s) 1849
MspI CCGG 1 cut(s) 2963
MspR9I CCNGG 3 cut(s) 654, 1403, 1886
Mva1269I GAATGC 1 cut(s) 2835
MvaI CCWGG 3 cut(s) 654, 1403, 1886
MvnI CGCG 1 cut(s) 2959
MwoI GCNNNNNNNGC 3 cut(s) 284, 1877, 2954
NcoI CCATGG 1 cut(s) 555
NlaIV GGNNCC 5 cut(s) 502, 862, 1400, 2067, 2493
NmuCI GTSAC 4 cut(s) 13, 656, 2398, 3043
NsiI ATGCAT 2 cut(s) 442, 2740
NspI RCATGY 7 cut(s) 361, 1367, 1754, 1791, 2144, 2565, 2751
NspV TTCGAA 1 cut(s) 2807
PaeR7I CTCGAG 2 cut(s) 323, 2089
PagI TCATGA 1 cut(s) 2476
PceI AGGCCT 1 cut(s) 1871
PciI ACATGT 2 cut(s) 1750, 2747
PciSI GCTCTTC 2 cut(s) 686, 1126
PctI GAATGC 1 cut(s) 2835
PdmI GAANNNNTTC 4 cut(s) 1419, 1613, 2114, 2177
PfeI GAWTC 7 cut(s) 581, 803, 1567, 2554, 2684, 2722, 2776
PkrI GCNGC 8 cut(s) 26, 280, 283, 286, 308, 2526, 2818, 2995
PleI GAGTC 7 cut(s) 69, 1026, 1157, 2449, 2764, 2974, 3040
PpsI GAGTC 7 cut(s) 69, 1026, 1157, 2449, 2764, 2974, 3040
PpuMI RGGWCCY 2 cut(s) 860, 893
PscI ACATGT 2 cut(s) 1750, 2747
PshAI GACNNNNGTC 1 cut(s) 2965
PshBI ATTAAT 1 cut(s) 776
PsiI TTATAA 1 cut(s) 2700
Psp5II RGGWCCY 2 cut(s) 860, 893
Psp6I CCWGG 3 cut(s) 652, 1401, 1884
PspEI GGTNACC 1 cut(s) 656
PspFI CCCAGC 1 cut(s) 2199
PspGI CCWGG 3 cut(s) 652, 1401, 1884
PspN4I GGNNCC 5 cut(s) 502, 862, 1400, 2067, 2493
PspPI GGNCC 6 cut(s) 149, 637, 860, 893, 1399, 2215
PspPPI RGGWCCY 2 cut(s) 860, 893
PstI CTGCAG 2 cut(s) 1923, 2821
PsuI RGATCY 2 cut(s) 1857, 2065
PvuII CAGCTG 1 cut(s) 1849
RsaI GTAC 8 cut(s) 376, 570, 752, 1721, 2289, 2487, 2606, 2792
RsaNI GTAC 8 cut(s) 375, 569, 751, 1720, 2288, 2486, 2605, 2791
RseI CAYNNNNRTG 2 cut(s) 973, 1243
SapI GCTCTTC 2 cut(s) 686, 1126
SatI GCNGC 8 cut(s) 25, 279, 282, 285, 307, 2525, 2817, 2994
Sau96I GGNCC 6 cut(s) 149, 637, 860, 893, 1399, 2215
SchI GAGTC 7 cut(s) 69, 1027, 1157, 2450, 2764, 2975, 3040
ScrFI CCNGG 3 cut(s) 654, 1403, 1886
SduI GDGCHC 1 cut(s) 825
SexAI ACCWGGT 1 cut(s) 652
SfaNI GCATC 7 cut(s) 694, 1603, 1698, 1779, 2353, 2725, 2747
SfcI CTRYAG 5 cut(s) 79, 204, 576, 1919, 2817
Sfr274I CTCGAG 2 cut(s) 323, 2089
SfuI TTCGAA 1 cut(s) 2807
SinI GGWCC 4 cut(s) 149, 860, 893, 1399
SlaI CTCGAG 2 cut(s) 323, 2089
SmiMI CAYNNNNRTG 2 cut(s) 973, 1243
SmlI CTYRAG 6 cut(s) 95, 323, 818, 1015, 1572, 2089
SmoI CTYRAG 6 cut(s) 95, 323, 818, 1015, 1572, 2089
SseBI AGGCCT 1 cut(s) 1871
SsiI CCGC 4 cut(s) 157, 619, 1107, 1768
SspMI CTAG 8 cut(s) 350, 405, 785, 926, 959, 1071, 1818, 2301
StuI AGGCCT 1 cut(s) 1871
StyD4I CCNGG 3 cut(s) 652, 1401, 1884
StyI CCWWGG 3 cut(s) 555, 1505, 2069
TaaI ACNGT 7 cut(s) 598, 770, 1937, 1984, 2125, 2287, 2324
TaqI TCGA 7 cut(s) 324, 625, 2090, 2646, 2761, 2807, 2899
TatI WGTACW 4 cut(s) 374, 750, 2485, 2604
TfiI GAWTC 7 cut(s) 581, 803, 1567, 2554, 2684, 2722, 2776
TscAI CASTG 4 cut(s) 830, 1165, 1502, 2436
TseFI GTSAC 4 cut(s) 13, 656, 2398, 3043
TseI GCWGC 8 cut(s) 24, 278, 281, 284, 306, 2524, 2816, 2993
Tsp45I GTSAC 4 cut(s) 13, 656, 2398, 3043
TspGWI ACGGA 1 cut(s) 1628
TspRI CASTG 4 cut(s) 830, 1165, 1502, 2436
VpaK11BI GGWCC 4 cut(s) 149, 860, 893, 1399
VspI ATTAAT 1 cut(s) 776
XagI CCTNNNNNAGG 1 cut(s) 1865
XapI RAATTY 8 cut(s) 488, 671, 1339, 1555, 1971, 2114, 2278, 2858
XbaI TCTAGA 1 cut(s) 349
XceI RCATGY 7 cut(s) 361, 1367, 1754, 1791, 2144, 2565, 2751
XhoI CTCGAG 2 cut(s) 323, 2089
XmiI GTMKAC 3 cut(s) 138, 1690, 1932
XmnI GAANNNNTTC 4 cut(s) 1419, 1613, 2114, 2177
XspI CTAG 8 cut(s) 350, 405, 785, 926, 959, 1071, 1818, 2301
Zsp2I ATGCAT 2 cut(s) 442, 2740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.