Rw5G044340

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000183
Physical Location & Seq
Reverse (-)
78034108 .. 78041002
6895 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G044340.1

Sequence Viewer

Length: 2988 bp
ATGGGTCGCTGCACTTTCTCCTCGGACGATATTATAATAAAGTCTCCGAATGATAAGAAATTGTACAGATTAATTGAGCTTGAGAATGGCCTCACTGCATTGCTCATTCATGATCCTGAGATTTACCCACAAGGGCCCAAAAGCCTTGAACCCAGTGAAGAAGATGAAGCAGCCAAAGGTGGAGATGGTGCCTCTGAGACTAAGACGGCAGCAGCAGCAATGTGCGTTGGAATAGGCAGCATGTCTGACCCTGTTGAGGCACAAGGGCTTGCACACTTCCTAGAACACATGCTCTTTATGGGGAGTACAAAGTTTCCTATTGAAAATGAGTTCGGTAGTTACTTGTCCAAGCATGGAGGATGGTCAAATGCATACACAGAAGCAGAGCAGACTTGCTACTACTTTGTTGTAAAACGAGAGTTTCTAAAGGGTGCCTTATCAAGATTTTCTCAGTTCTTTGTTTCACCTCTAATGAAAAGAGAAGCCATGGAGCGAGAGTTACAGGCTATAGATTCAGAGTTTAACAGGGTTCTGCAAAACGATTTTTGCCGCCTTCAGCAACTTCAATGCTATACATCCTCACCTGGTCACCCATTTAACAAATTCTCTTGGGGAAATAAGAAGAGCTTGGTTAATGCAAAAGAAAAAGGGATCGACTTGCGTGAACAAATAATGAAATTTTACAGTGACTATTACCATGGTGGATTAATGAAGCTAGTTGTCATTGGTGGAGAATCTCTTGATGTACTTGAGGAGTGGATTTTAGAACTGTATGGAGATGTTAAAAGTGGTCCCCAAGTAAATATGGAGTTCAAGGCAGAAGGTCCTATATGGAAATCTGGAAAACTTTACAGGCTAGAGGCTGTTGACAATGTTCATATACTCCACTTAGCATGGACACTTCCATGTCTTCATCGACACTATTTGAAAAGCCCAACATATTATTTACGTCATCTCCTTGAGCATGAGGGCAGGGGAAGTTTGTATTTCCACCTCAAAGCTAGAGGTTGGGCAACATTTGTAAGTGCTCCAGTGGAACACTATTCTGTGGCTGATGTCTTCTGCATGATCATATACCTCACTGACTCTGGATTGGAGAAGATTTTCGATATAATTGGGTTGGTGTATCAATACATTAAGTTCTTACGGCAAGTGTTGCCCCAAGAATGGATATTTCGTGAACTCCAGGATATTGGGAACATCTACCTTAAGTTTTTAGAGGAGCAGCCCCAGGATTATTATGCTTTAAGACTTGGAGGAAATTTACTACATTATCCCGCAGAGCATGCTATTTATGGGGACTATTTGCTAGAGAATTGGGATGAGAAATTGATAGAATATACTCTAGGTTTCTTGAGACCAGAAAACATGAGGATTGATGTGATATCTAAGTCCTCCACGTTGTCAGAAGATTTCCAGTGCGAGCCTTGGTTTGGGTCACATTTTACCGAGGAAGATATATCTCCGTCTTTGATAGATTTATGGAAGGATCCTCAAGAAATTGATAGTTCATTGCATCTCCCAGAAAAGAATGAATTCATTCCCTCTGATTTTTCCATCCGTTCTGATGTTCTTGATACTGCAAATACATCTTGTCCTAGATGTATACTTGATGGGCCATTGGTGAAGTTATGGTACAAGCTTGATAGTACTTACAAACTTCCGCAGGCAAACATATACTTTCGTGTCAGTCTAAAGGAAGCATCTGGTAGTGTGAAGAGTTCTGTCTTGACTCAACTATATGGCGACCTTCTTTGGGATGAGCTGAATGAGGTTGTATATCAGGCTAGTGTCGCCCAGCTGGGAACTTCTGTATCTGTGTACACTAACTATCTGGAGCTGAAGGTCAGCGGTTTCAATGATAAGATTCCAACTCTCTTGTCAAAAATTATGACAACAGCCAAAAATTTCTCGCCAACTTACGAATGTTTCAAGGTTGTTAAAGAGGACATGGAGCGAGCATATAAGAACACCAATATGGATCCTTGGAGCCACTCGACATACTTGAGAGATGAAGTTTTGTACCAAAATTTCGGTGATGTAGATGAGACGTTGCATGTTTTAAATGGATTGACTGTTTCTGATGTGAAGTCATTCATTCCCGAGCTTTGGTCCCCGCATTACATTGAAGGCCTTTGCCATGGAAATCTGTTAGAAGAAGAAGCAATCAGCCTTTTAAATATAGTGAAGACAAATTTTTCTGTGCAACCACATCCTATCGAACTGAAGCATAAAAATAACTGTATTTGTCTTCCTCCTGATGCTAACGTCATTAGAGATGTCAGAGTGAAGAATAAGTCTCAAACAAACTCTGTTATCGAGCTGTATTTTCAAATTGAGTGGGCAGTGGGGATTGAGTCCACCAGATTGAGAGCATTGATAGATCTCTTTTGTGAAATTGTAAACGAACCACTTTACAATCAGCTAAGGACGAAGGAGCAGCTGGGATATGTTGTTCAATGTAACTGGAATGTAACACTCGGTGTTTTTGGCTTTTATTTTAAAGTTCAGTCGTCTGATTATAATCCAATCTACTTACAAAGAAGAGTTGACAACTTTATCAATGGTGTGGAAGAACTTTTGCTAGGATTGGATGATGAATCCTTTGAGAATTACCAAGGTGGGTTAATAGCGAAGCTATTGGAGAAAGATTCAACCCTTATGGATGAAACTGCTCGATATTGGGATCATATTATCTGCAAAAGGTATAAGTTTGACAAATGGGAAAAGATAGCAGAAGAACTCAGATGTCTACAGAAGGGGGATGTTATCAAATTTTACAAGACTTACTTGCAACTATCATCTCCCAAGCGTCGGAGACTTGTAACTCGTGTTTGGGGTTGCAACACTGACATGAAAGAAGCTGAAGAAGAGCGAACGGAGTCTGAGCAAATCATTGAAGACCTTGCAGCCTTTAAGAGGTCATCTCAGTTCTATGGTCATGGAAATTGTCAGACAGTTGCTCCTCCGATTATGTGTAAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

995

Amino Acids

114.72

Weight (kDa)

5.27

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 66 - 181 3.2e-31 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 221 - 396 1.1e-17 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 404 - 685 2.7e-80 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 691 - 873 1.9e-18 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 796 - 895 7.6e-24 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 35, 2523
AccB1I GGYRCC 2 cut(s) 188, 431
AccI GTMKAC 2 cut(s) 1606, 2752
AciI CCGC 5 cut(s) 550, 1278, 1664, 1851, 2117
AclWI GGATC 7 cut(s) 107, 659, 1484, 1497, 1976, 1989, 2694
AcsI RAATTY 8 cut(s) 602, 677, 1261, 1535, 1906, 2029, 2194, 2774
AcuI CTGAAG 4 cut(s) 539, 1862, 2246, 2886
AdeI CACNNNGTG 1 cut(s) 2483
AfaI GTAC 7 cut(s) 65, 307, 747, 1637, 1651, 1823, 2024
AfiI CCNNNNNNNGG 7 cut(s) 256, 1167, 1433, 1756, 2109, 2814, 2919
AflII CTTAAG 1 cut(s) 1208
AjnI CCWGG 3 cut(s) 583, 1185, 1230
AloI GAACNNNNNNTCC 2 cut(s) 2439, 2471
Alw21I GWGCWC 1 cut(s) 1030
Alw26I GTCTC 6 cut(s) 48, 191, 1351, 2042, 2304, 2812
AlwI GGATC 7 cut(s) 107, 659, 1484, 1497, 1976, 1989, 2694
Ama87I CYCGRG 1 cut(s) 2102
AoxI GGCC 4 cut(s) 88, 134, 1616, 2131
ApaI GGGCCC 1 cut(s) 138
ApeKI GCWGC 9 cut(s) 9, 170, 209, 212, 215, 237, 1225, 2440, 2909
ApoI RAATTY 8 cut(s) 602, 677, 1261, 1535, 1906, 2029, 2194, 2774
AseI ATTAAT 2 cut(s) 71, 707
Asp700I GAANNNNTTC 4 cut(s) 1103, 1535, 1539, 2093
AspS9I GGNCC 6 cut(s) 134, 135, 791, 824, 1616, 2112
AsuHPI GGTGA 5 cut(s) 456, 573, 581, 1636, 2048
AvaI CYCGRG 1 cut(s) 2102
AvaII GGWCC 3 cut(s) 791, 824, 2112
BaeGI GKGCMC 1 cut(s) 138
BaeI ACNNNNGTAYC 2 cut(s) 1797, 1830
BamHI GGATCC 2 cut(s) 1489, 1981
BanI GGYRCC 2 cut(s) 188, 431
BanII GRGCYC 1 cut(s) 138
BarI GAAGNNNNNNTAC 2 cut(s) 1619, 1651
BauI CACGAG 1 cut(s) 2829
BbsI GAAGAC 5 cut(s) 902, 1051, 2195, 2243, 2907
Bbv12I GWGCWC 1 cut(s) 1030
BbvI GCAGC 8 cut(s) 182, 221, 224, 227, 249, 1237, 2452, 2921
BccI CCATC 4 cut(s) 179, 354, 1565, 1607
BceAI ACGGC 2 cut(s) 222, 1163
BciT130I CCWGG 3 cut(s) 585, 1187, 1232
BclI TGATCA 1 cut(s) 1068
BcoDI GTCTC 6 cut(s) 48, 191, 1351, 2042, 2304, 2812
BfaI CTAG 9 cut(s) 281, 716, 857, 1002, 1310, 1346, 1599, 1788, 2585
BfmI CTRYAG 2 cut(s) 507, 2753
BfrI CTTAAG 1 cut(s) 1208
BglII AGATCT 1 cut(s) 2383
BmcAI AGTACT 1 cut(s) 1651
Bme1390I CCNGG 3 cut(s) 585, 1187, 1232
Bme18I GGWCC 3 cut(s) 791, 824, 2112
BmeT110I CYCGRG 1 cut(s) 2102
BmgT120I GGNCC 6 cut(s) 134, 135, 791, 824, 1616, 2112
BmiI GGNNCC 8 cut(s) 136, 190, 433, 793, 1491, 1983, 1991, 2114
BmrFI CCNGG 3 cut(s) 585, 1187, 1232
BmrI ACTGGG 1 cut(s) 147
BmsI GCATC 3 cut(s) 1525, 1712, 2251
BmuI ACTGGG 1 cut(s) 147
BpiI GAAGAC 5 cut(s) 902, 1051, 2195, 2243, 2907
BplI GAGNNNNNCTC 2 cut(s) 2911, 2943
BpmI CTGGAG 3 cut(s) 1014, 1169, 1856
Bpu10I CCTNAGC 1 cut(s) 2426
BpuEI CTTGAG 6 cut(s) 101, 770, 980, 1375, 1479, 2026
BsaBI GATNNNNATC 1 cut(s) 2523
BsaI GGTCTC 1 cut(s) 1351
BsaJI CCNNGG 9 cut(s) 21, 486, 697, 1230, 1427, 1449, 1985, 2140, 2617
BsaXI ACNNNNNCTCC 6 cut(s) 482, 512, 939, 969, 2947, 2977
Bsc4I CCNNNNNNNGG 7 cut(s) 256, 1167, 1433, 1756, 2109, 2814, 2919
Bse1I ACTGG 4 cut(s) 153, 1031, 1417, 2471
Bse3DI GCAATG 3 cut(s) 98, 225, 1511
Bse8I GATNNNNATC 1 cut(s) 2523
BseBI CCWGG 3 cut(s) 585, 1187, 1232
BseDI CCNNGG 9 cut(s) 21, 486, 697, 1230, 1427, 1449, 1985, 2140, 2617
BseGI GGATG 9 cut(s) 365, 575, 1327, 1557, 1765, 2212, 2599, 2671, 2770
BseJI GATNNNNATC 1 cut(s) 2523
BseLI CCNNNNNNNGG 7 cut(s) 256, 1167, 1433, 1756, 2109, 2814, 2919
BseMI GCAATG 3 cut(s) 98, 225, 1511
BseMII CTCAG 6 cut(s) 108, 186, 464, 2758, 2877, 2942
BseNI ACTGG 4 cut(s) 153, 1031, 1417, 2471
BseRI GAGGAG 4 cut(s) 10, 767, 1235, 2955
BseSI GKGCMC 1 cut(s) 138
BseXI GCAGC 8 cut(s) 182, 221, 224, 227, 249, 1237, 2452, 2921
BseYI CCCAGC 3 cut(s) 1797, 1801, 2443
BshFI GGCC 4 cut(s) 90, 136, 1618, 2133
BshNI GGYRCC 2 cut(s) 188, 431
BsiHKAI GWGCWC 1 cut(s) 1030
BsiHKCI CYCGRG 1 cut(s) 2102
BslFI GGGAC 3 cut(s) 777, 1313, 2098
BslI CCNNNNNNNGG 7 cut(s) 256, 1167, 1433, 1756, 2109, 2814, 2919
BsmAI GTCTC 6 cut(s) 48, 191, 1351, 2042, 2304, 2812
BsmBI CGTCTC 1 cut(s) 2042
BsmFI GGGAC 3 cut(s) 777, 1313, 2098
BsnI GGCC 4 cut(s) 90, 136, 1618, 2133
Bso31I GGTCTC 1 cut(s) 1351
BsoBI CYCGRG 1 cut(s) 2102
Bsp120I GGGCCC 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 138, 1030
Bsp1407I TGTACA 2 cut(s) 63, 1821
Bsp143I GATC 7 cut(s) 112, 651, 1068, 1489, 1981, 2383, 2686
Bsp19I CCATGG 3 cut(s) 486, 697, 2140
BspACI CCGC 5 cut(s) 550, 1278, 1664, 1851, 2117
BspANI GGCC 4 cut(s) 90, 136, 1618, 2133
BspCNI CTCAG 6 cut(s) 109, 187, 463, 2757, 2878, 2941
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 8 cut(s) 136, 190, 433, 793, 1491, 1983, 1991, 2114
BspPI GGATC 7 cut(s) 107, 659, 1484, 1497, 1976, 1989, 2694
BspQI GCTCTTC 2 cut(s) 617, 2865
BspT107I GGYRCC 2 cut(s) 188, 431
BspTI CTTAAG 1 cut(s) 1208
BspTNI GGTCTC 1 cut(s) 1351
BsrDI GCAATG 3 cut(s) 98, 225, 1511
BsrGI TGTACA 2 cut(s) 63, 1821
BsrI ACTGG 4 cut(s) 153, 1031, 1417, 2471
BssECI CCNNGG 9 cut(s) 21, 486, 697, 1230, 1427, 1449, 1985, 2140, 2617
BssMI GATC 7 cut(s) 112, 651, 1068, 1489, 1981, 2383, 2686
BssNAI GTATAC 1 cut(s) 1607
BssSI CACGAG 1 cut(s) 2829
BssT1I CCWWGG 6 cut(s) 486, 697, 1427, 1985, 2140, 2617
Bst1107I GTATAC 1 cut(s) 1607
Bst2BI CACGAG 1 cut(s) 2829
Bst2UI CCWGG 3 cut(s) 585, 1187, 1232
Bst4CI ACNGT 5 cut(s) 686, 771, 2077, 2243, 2959
Bst6I CTCTTC 4 cut(s) 617, 1712, 2539, 2865
BstAFI CTTAAG 1 cut(s) 1208
BstAPI GCANNNNNTGC 2 cut(s) 1156, 1286
BstAUI TGTACA 2 cut(s) 63, 1821
BstC8I GCNNGC 5 cut(s) 270, 1287, 1424, 1668, 1959
BstDSI CCRYGG 3 cut(s) 486, 697, 2140
BstEII GGTNACC 1 cut(s) 587
BstENI CCTNNNNNAGG 1 cut(s) 2917
BstF5I GGATG 9 cut(s) 365, 575, 1327, 1557, 1765, 2212, 2599, 2671, 2770
BstKTI GATC 7 cut(s) 115, 654, 1071, 1492, 1984, 2386, 2689
BstMAI GTCTC 6 cut(s) 48, 191, 1351, 2042, 2304, 2812
BstMBI GATC 7 cut(s) 112, 651, 1068, 1489, 1981, 2383, 2686
BstMWI GCNNNNNNNGC 4 cut(s) 215, 1156, 1286, 1793
BstNI CCWGG 3 cut(s) 585, 1187, 1232
BstNSI RCATGY 4 cut(s) 244, 292, 1289, 2060
BstPI GGTNACC 1 cut(s) 587
BstSCI CCNGG 3 cut(s) 583, 1185, 1230
BstSFI CTRYAG 2 cut(s) 507, 2753
BstSLI GKGCMC 1 cut(s) 138
BstV1I GCAGC 8 cut(s) 182, 221, 224, 227, 249, 1237, 2452, 2921
BstV2I GAAGAC 5 cut(s) 902, 1051, 2195, 2243, 2907
BstX2I RGATCY 3 cut(s) 1489, 1981, 2383
BstXI CCANNNNNNTGG 1 cut(s) 1193
BstYI RGATCY 3 cut(s) 1489, 1981, 2383
BstZ17I GTATAC 1 cut(s) 1607
BsuRI GGCC 4 cut(s) 90, 136, 1618, 2133
BtgI CCRYGG 3 cut(s) 486, 697, 2140
BtsCI GGATG 9 cut(s) 365, 575, 1327, 1557, 1765, 2212, 2599, 2671, 2770
BtsI GCAGTG 2 cut(s) 93, 2352
BtsIMutI CAGTG 8 cut(s) 93, 160, 691, 1038, 1080, 1424, 2352, 2847
Cac8I GCNNGC 5 cut(s) 270, 1287, 1424, 1668, 1959
CciI TCATGA 1 cut(s) 109
Cfr13I GGNCC 6 cut(s) 134, 135, 791, 824, 1616, 2112
CseI GACGC 1 cut(s) 2801
CsiI ACCWGGT 1 cut(s) 583
Csp6I GTAC 7 cut(s) 64, 306, 746, 1636, 1650, 1822, 2023
CspCI CAANNNNNGTGG 2 cut(s) 2321, 2356
CviQI GTAC 7 cut(s) 64, 306, 746, 1636, 1650, 1822, 2023
DpnI GATC 7 cut(s) 114, 653, 1070, 1491, 1983, 2385, 2688
DpnII GATC 7 cut(s) 112, 651, 1068, 1489, 1981, 2383, 2686
DraI TTTAAA 3 cut(s) 2064, 2178, 2503
DraIII CACNNNGTG 1 cut(s) 2483
Eam1104I CTCTTC 4 cut(s) 617, 1712, 2539, 2865
EarI CTCTTC 4 cut(s) 617, 1712, 2539, 2865
Eco130I CCWWGG 6 cut(s) 486, 697, 1427, 1985, 2140, 2617
Eco147I AGGCCT 1 cut(s) 2133
Eco24I GRGCYC 1 cut(s) 138
Eco31I GGTCTC 1 cut(s) 1351
Eco32I GATATC 1 cut(s) 1386
Eco47I GGWCC 3 cut(s) 791, 824, 2112
Eco57I CTGAAG 4 cut(s) 539, 1862, 2246, 2886
Eco88I CYCGRG 1 cut(s) 2102
Eco91I GGTNACC 1 cut(s) 587
EcoNI CCTNNNNNAGG 1 cut(s) 2917
EcoO109I RGGNCCY 2 cut(s) 134, 824
EcoO65I GGTNACC 1 cut(s) 587
EcoRI GAATTC 1 cut(s) 1535
EcoRII CCWGG 3 cut(s) 583, 1185, 1230
EcoRV GATATC 1 cut(s) 1386
EcoT14I CCWWGG 6 cut(s) 486, 697, 1427, 1985, 2140, 2617
EcoT22I ATGCAT 1 cut(s) 373
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 6 cut(s) 486, 697, 1427, 1985, 2140, 2617
Esp3I CGTCTC 1 cut(s) 2042
FalI AAGNNNNNCTT 6 cut(s) 419, 451, 611, 643, 2774, 2806
FaqI GGGAC 3 cut(s) 777, 1313, 2098
FauI CCCGC 2 cut(s) 1285, 2124
FbaI TGATCA 1 cut(s) 1068
FblI GTMKAC 2 cut(s) 1606, 2752
FokI GGATG 9 cut(s) 372, 562, 1334, 1544, 1772, 2199, 2606, 2678, 2777
FriOI GRGCYC 1 cut(s) 138
FspBI CTAG 9 cut(s) 281, 716, 857, 1002, 1310, 1346, 1599, 1788, 2585
GsaI CCCAGC 3 cut(s) 1801, 1805, 2447
GsuI CTGGAG 3 cut(s) 1014, 1169, 1856
HaeIII GGCC 4 cut(s) 90, 136, 1618, 2133
HgaI GACGC 1 cut(s) 2801
HincII GTYRAC 2 cut(s) 868, 2551
HindII GTYRAC 2 cut(s) 868, 2551
HindIII AAGCTT 2 cut(s) 1640, 2981
HinfI GANTC 9 cut(s) 512, 734, 1085, 1732, 1867, 2357, 2600, 2651, 2882
HphI GGTGA 5 cut(s) 456, 573, 581, 1636, 2048
Hpy99I CGWCG 1 cut(s) 2817
HpyAV CCTTC 8 cut(s) 563, 815, 1480, 1760, 1837, 2123, 2428, 2752
HpyCH4III ACNGT 5 cut(s) 686, 771, 2077, 2243, 2959
HpyCH4IV ACGT 4 cut(s) 949, 1400, 2051, 2268
HpyF10VI GCNNNNNNNGC 4 cut(s) 215, 1156, 1286, 1793
HpySE526I ACGT 4 cut(s) 949, 1400, 2051, 2268
Ksp22I TGATCA 1 cut(s) 1068
Kzo9I GATC 7 cut(s) 112, 651, 1068, 1489, 1981, 2383, 2686
LguI GCTCTTC 2 cut(s) 617, 2865
LmnI GCTCC 8 cut(s) 490, 1033, 1222, 1837, 1954, 1989, 2437, 2968
Lsp1109I GCAGC 8 cut(s) 182, 221, 224, 227, 249, 1237, 2452, 2921
LweI GCATC 3 cut(s) 1525, 1712, 2251
MabI ACCWGGT 1 cut(s) 583
MaeI CTAG 9 cut(s) 281, 716, 857, 1002, 1310, 1346, 1599, 1788, 2585
MaeII ACGT 4 cut(s) 949, 1400, 2051, 2268
MaeIII GTNAC 8 cut(s) 338, 498, 587, 686, 1437, 2462, 2473, 2824
MalI GATC 7 cut(s) 114, 653, 1070, 1491, 1983, 2385, 2688
MboI GATC 7 cut(s) 112, 651, 1068, 1489, 1981, 2383, 2686
MflI RGATCY 3 cut(s) 1489, 1981, 2383
MhlI GDGCHC 2 cut(s) 138, 1030
MlyI GAGTC 4 cut(s) 1079, 1726, 2366, 2891
MmeI TCCRAC 3 cut(s) 208, 1895, 2795
Mph1103I ATGCAT 1 cut(s) 373
MroXI GAANNNNTTC 4 cut(s) 1103, 1535, 1539, 2093
MslI CAYNNNNRTG 3 cut(s) 904, 1976, 2852
MspA1I CMGCKG 3 cut(s) 1801, 1851, 2443
MspCI CTTAAG 1 cut(s) 1208
MspR9I CCNGG 3 cut(s) 585, 1187, 1232
MvaI CCWGG 3 cut(s) 585, 1187, 1232
MwoI GCNNNNNNNGC 4 cut(s) 215, 1156, 1286, 1793
NcoI CCATGG 3 cut(s) 486, 697, 2140
NdeII GATC 7 cut(s) 112, 651, 1068, 1489, 1981, 2383, 2686
NlaIV GGNNCC 8 cut(s) 136, 190, 433, 793, 1491, 1983, 1991, 2114
NmuCI GTSAC 3 cut(s) 587, 686, 1437
NsiI ATGCAT 1 cut(s) 373
NspI RCATGY 4 cut(s) 244, 292, 1289, 2060
PaeI GCATGC 1 cut(s) 1289
PagI TCATGA 1 cut(s) 109
PceI AGGCCT 1 cut(s) 2133
PciSI GCTCTTC 2 cut(s) 617, 2865
PdmI GAANNNNTTC 4 cut(s) 1103, 1535, 1539, 2093
PfeI GAWTC 5 cut(s) 512, 734, 1867, 2600, 2651
PfoI TCCNGGA 1 cut(s) 1185
PleI GAGTC 4 cut(s) 1079, 1726, 2365, 2890
PpsI GAGTC 4 cut(s) 1079, 1726, 2365, 2890
PpuMI RGGWCCY 1 cut(s) 824
PshBI ATTAAT 2 cut(s) 71, 707
PsiI TTATAA 2 cut(s) 35, 2523
Psp5II RGGWCCY 1 cut(s) 824
Psp6I CCWGG 3 cut(s) 583, 1185, 1230
PspEI GGTNACC 1 cut(s) 587
PspFI CCCAGC 3 cut(s) 1797, 1801, 2443
PspGI CCWGG 3 cut(s) 583, 1185, 1230
PspN4I GGNNCC 8 cut(s) 136, 190, 433, 793, 1491, 1983, 1991, 2114
PspOMI GGGCCC 1 cut(s) 134
PspPI GGNCC 6 cut(s) 134, 135, 791, 824, 1616, 2112
PspPPI RGGWCCY 1 cut(s) 824
PsuI RGATCY 3 cut(s) 1489, 1981, 2383
PvuII CAGCTG 2 cut(s) 1801, 2443
RsaI GTAC 7 cut(s) 65, 307, 747, 1637, 1651, 1823, 2024
RsaNI GTAC 7 cut(s) 64, 306, 746, 1636, 1650, 1822, 2023
RseI CAYNNNNRTG 3 cut(s) 904, 1976, 2852
SapI GCTCTTC 2 cut(s) 617, 2865
Sau3AI GATC 7 cut(s) 112, 651, 1068, 1489, 1981, 2383, 2686
Sau96I GGNCC 6 cut(s) 134, 135, 791, 824, 1616, 2112
ScaI AGTACT 1 cut(s) 1651
SchI GAGTC 4 cut(s) 1079, 1726, 2366, 2891
ScrFI CCNGG 3 cut(s) 585, 1187, 1232
SduI GDGCHC 2 cut(s) 138, 1030
SexAI ACCWGGT 1 cut(s) 583
SfaNI GCATC 3 cut(s) 1525, 1712, 2251
SfcI CTRYAG 2 cut(s) 507, 2753
SinI GGWCC 3 cut(s) 791, 824, 2112
SmiMI CAYNNNNRTG 3 cut(s) 904, 1976, 2852
SmlI CTYRAG 7 cut(s) 80, 749, 959, 1208, 1354, 1494, 2005
SmoI CTYRAG 7 cut(s) 80, 749, 959, 1208, 1354, 1494, 2005
SphI GCATGC 1 cut(s) 1289
SseBI AGGCCT 1 cut(s) 2133
SsiI CCGC 5 cut(s) 550, 1278, 1664, 1851, 2117
SspMI CTAG 9 cut(s) 281, 716, 857, 1002, 1310, 1346, 1599, 1788, 2585
StuI AGGCCT 1 cut(s) 2133
StyD4I CCNGG 3 cut(s) 583, 1185, 1230
StyI CCWWGG 6 cut(s) 486, 697, 1427, 1985, 2140, 2617
TaaI ACNGT 5 cut(s) 686, 771, 2077, 2243, 2959
TaiI ACGT 4 cut(s) 952, 1403, 2054, 2271
TaqI TCGA 7 cut(s) 654, 916, 1107, 1997, 2220, 2319, 2677
TatI WGTACW 5 cut(s) 63, 305, 745, 1649, 1821
TauI GCSGC 1 cut(s) 552
TfiI GAWTC 5 cut(s) 512, 734, 1867, 2600, 2651
TscAI CASTG 8 cut(s) 100, 160, 691, 1038, 1087, 1424, 2352, 2854
TseFI GTSAC 3 cut(s) 587, 686, 1437
TseI GCWGC 9 cut(s) 9, 170, 209, 212, 215, 237, 1225, 2440, 2909
Tsp45I GTSAC 3 cut(s) 587, 686, 1437
TspGWI ACGGA 3 cut(s) 1455, 1550, 2894
TspRI CASTG 8 cut(s) 100, 160, 691, 1038, 1087, 1424, 2352, 2854
Vha464I CTTAAG 1 cut(s) 1208
VpaK11BI GGWCC 3 cut(s) 791, 824, 2112
VspI ATTAAT 2 cut(s) 71, 707
XagI CCTNNNNNAGG 1 cut(s) 2917
XapI RAATTY 8 cut(s) 602, 677, 1261, 1535, 1906, 2029, 2194, 2774
XceI RCATGY 4 cut(s) 244, 292, 1289, 2060
XmiI GTMKAC 2 cut(s) 1606, 2752
XmnI GAANNNNTTC 4 cut(s) 1103, 1535, 1539, 2093
XspI CTAG 9 cut(s) 281, 716, 857, 1002, 1310, 1346, 1599, 1788, 2585
ZrmI AGTACT 1 cut(s) 1651
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.