Belongs to the peptidase M16 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1203 |
| Acc36I | ACCTGC | 1 cut(s) | 146 |
| AccI | GTMKAC | 2 cut(s) | 439, 741 |
| AciI | CCGC | 2 cut(s) | 517, 1320 |
| AclWI | GGATC | 4 cut(s) | 271, 601, 869, 882 |
| AcoI | YGGCCR | 2 cut(s) | 1051, 1218 |
| AcsI | RAATTY | 3 cut(s) | 13, 780, 984 |
| AcuI | CTGAAG | 2 cut(s) | 548, 1566 |
| AfaI | GTAC | 4 cut(s) | 470, 484, 1109, 1297 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 616, 979 |
| AflII | CTTAAG | 1 cut(s) | 1499 |
| AgsI | TTSAA | 7 cut(s) | 21, 751, 920, 1127, 1312, 1528, 1580 |
| AjnI | CCWGG | 3 cut(s) | 55, 142, 704 |
| AluBI | AGCT | 9 cut(s) | 69, 475, 598, 641, 760, 767, 854, 1030, 1544 |
| AluI | AGCT | 9 cut(s) | 69, 475, 598, 641, 760, 767, 854, 1030, 1544 |
| Alw26I | GTCTC | 3 cut(s) | 631, 636, 731 |
| AlwI | GGATC | 4 cut(s) | 271, 601, 869, 882 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1282 |
| AoxI | GGCC | 5 cut(s) | 250, 1051, 1218, 1317, 1495 |
| ApeKI | GCWGC | 2 cut(s) | 1027, 1589 |
| ApoI | RAATTY | 3 cut(s) | 13, 780, 984 |
| AseI | ATTAAT | 1 cut(s) | 330 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 51, 362, 674 |
| AspLEI | GCGC | 1 cut(s) | 1507 |
| BaeGI | GKGCMC | 1 cut(s) | 246 |
| BalI | TGGCCA | 2 cut(s) | 1053, 1220 |
| BamHI | GGATCC | 1 cut(s) | 874 |
| BauI | CACGAG | 2 cut(s) | 372, 1416 |
| BbsI | GAAGAC | 4 cut(s) | 846, 1411, 1535, 1587 |
| BbvI | GCAGC | 2 cut(s) | 1039, 1601 |
| BccI | CCATC | 5 cut(s) | 37, 301, 392, 660, 1145 |
| BceAI | ACGGC | 1 cut(s) | 140 |
| BciT130I | CCWGG | 3 cut(s) | 57, 144, 706 |
| BciVI | GTATCC | 4 cut(s) | 114, 127, 143, 208 |
| BclI | TGATCA | 1 cut(s) | 207 |
| BcoDI | GTCTC | 3 cut(s) | 631, 636, 731 |
| BfaI | CTAG | 2 cut(s) | 642, 1464 |
| BfmI | CTRYAG | 1 cut(s) | 989 |
| BfoI | RGCGCY | 1 cut(s) | 1508 |
| BfrI | CTTAAG | 1 cut(s) | 1499 |
| BfuAI | ACCTGC | 1 cut(s) | 146 |
| BfuI | GTATCC | 4 cut(s) | 114, 127, 143, 208 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1494 |
| BisI | GCNGC | 3 cut(s) | 1028, 1320, 1590 |
| BlsI | GCNGC | 3 cut(s) | 1029, 1321, 1591 |
| Bme1390I | CCNGG | 3 cut(s) | 57, 144, 706 |
| BmiI | GGNNCC | 3 cut(s) | 7, 876, 996 |
| BmrFI | CCNGG | 3 cut(s) | 57, 144, 706 |
| BmsI | GCATC | 4 cut(s) | 352, 528, 1228, 1250 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1561 |
| BpiI | GAAGAC | 4 cut(s) | 846, 1411, 1535, 1587 |
| BpmI | CTGGAG | 2 cut(s) | 78, 254 |
| Bpu10I | CCTNAGC | 1 cut(s) | 159 |
| BpuEI | CTTGAG | 1 cut(s) | 919 |
| Bsa29I | ATCGAT | 1 cut(s) | 1264 |
| BsaJI | CCNNGG | 5 cut(s) | 142, 253, 705, 878, 1221 |
| BsaWI | WCCGGW | 1 cut(s) | 1558 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 616, 979 |
| Bse118I | RCCGGY | 1 cut(s) | 349 |
| Bse1I | ACTGG | 3 cut(s) | 237, 571, 1140 |
| Bse3DI | GCAATG | 2 cut(s) | 338, 1075 |
| BseBI | CCWGG | 3 cut(s) | 57, 144, 706 |
| BseCI | ATCGAT | 1 cut(s) | 1264 |
| BseDI | CCNNGG | 5 cut(s) | 142, 253, 705, 878, 1221 |
| BseGI | GGATG | 4 cut(s) | 384, 1100, 1241, 1357 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 616, 979 |
| BseMI | GCAATG | 2 cut(s) | 338, 1075 |
| BseMII | CTCAG | 4 cut(s) | 120, 267, 1289, 1345 |
| BseNI | ACTGG | 3 cut(s) | 237, 571, 1140 |
| BseRI | GAGGAG | 3 cut(s) | 164, 167, 1625 |
| BseSI | GKGCMC | 1 cut(s) | 246 |
| BseXI | GCAGC | 2 cut(s) | 1039, 1601 |
| BsgI | GTGCAG | 1 cut(s) | 172 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1559 |
| BshFI | GGCC | 5 cut(s) | 252, 1053, 1220, 1319, 1497 |
| BshVI | ATCGAT | 1 cut(s) | 1264 |
| BsiEI | CGRYCG | 1 cut(s) | 1559 |
| BsiSI | CCGG | 2 cut(s) | 350, 1559 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 616, 979 |
| BsmAI | GTCTC | 3 cut(s) | 631, 636, 731 |
| BsmBI | CGTCTC | 1 cut(s) | 636 |
| BsmI | GAATGC | 2 cut(s) | 1338, 1458 |
| BsnI | GGCC | 5 cut(s) | 252, 1053, 1220, 1319, 1497 |
| Bsp1286I | GDGCHC | 1 cut(s) | 246 |
| Bsp143I | GATC | 7 cut(s) | 207, 263, 592, 606, 817, 874, 904 |
| BspACI | CCGC | 2 cut(s) | 517, 1320 |
| BspANI | GGCC | 5 cut(s) | 252, 1053, 1220, 1319, 1497 |
| BspCNI | CTCAG | 4 cut(s) | 119, 268, 1288, 1344 |
| BspDI | ATCGAT | 1 cut(s) | 1264 |
| BspLI | GGNNCC | 3 cut(s) | 7, 876, 996 |
| BspMI | ACCTGC | 1 cut(s) | 146 |
| BspPI | GGATC | 4 cut(s) | 271, 601, 869, 882 |
| BspQI | GCTCTTC | 1 cut(s) | 76 |
| BspTI | CTTAAG | 1 cut(s) | 1499 |
| BsrDI | GCAATG | 2 cut(s) | 338, 1075 |
| BsrFI | RCCGGY | 1 cut(s) | 349 |
| BsrI | ACTGG | 3 cut(s) | 237, 571, 1140 |
| BssAI | RCCGGY | 1 cut(s) | 349 |
| BssECI | CCNNGG | 5 cut(s) | 142, 253, 705, 878, 1221 |
| BssMI | GATC | 7 cut(s) | 207, 263, 592, 606, 817, 874, 904 |
| BssNAI | GTATAC | 2 cut(s) | 440, 742 |
| BssSI | CACGAG | 2 cut(s) | 372, 1416 |
| BssT1I | CCWWGG | 3 cut(s) | 253, 878, 1221 |
| Bst1107I | GTATAC | 2 cut(s) | 440, 742 |
| Bst2BI | CACGAG | 2 cut(s) | 372, 1416 |
| Bst2UI | CCWGG | 3 cut(s) | 57, 144, 706 |
| Bst4CI | ACNGT | 3 cut(s) | 482, 746, 912 |
| Bst6I | CTCTTC | 4 cut(s) | 76, 545, 932, 1155 |
| BstAFI | CTTAAG | 1 cut(s) | 1499 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 697 |
| BstC8I | GCNNGC | 4 cut(s) | 351, 702, 852, 930 |
| BstDEI | CTNAG | 6 cut(s) | 106, 159, 276, 1013, 1275, 1331 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 614, 977 |
| BstF5I | GGATG | 4 cut(s) | 384, 1100, 1241, 1357 |
| BstH2I | RGCGCY | 1 cut(s) | 1508 |
| BstHHI | GCGC | 1 cut(s) | 1507 |
| BstKTI | GATC | 7 cut(s) | 210, 266, 595, 609, 820, 877, 907 |
| BstMAI | GTCTC | 3 cut(s) | 631, 636, 731 |
| BstMBI | GATC | 7 cut(s) | 207, 263, 592, 606, 817, 874, 904 |
| BstMCI | CGRYCG | 1 cut(s) | 1559 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 697, 1494, 1550 |
| BstNI | CCWGG | 3 cut(s) | 57, 144, 706 |
| BstNSI | RCATGY | 1 cut(s) | 695 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1561 |
| BstSCI | CCNGG | 3 cut(s) | 55, 142, 704 |
| BstSFI | CTRYAG | 1 cut(s) | 989 |
| BstSLI | GKGCMC | 1 cut(s) | 246 |
| BstV1I | GCAGC | 2 cut(s) | 1039, 1601 |
| BstV2I | GAAGAC | 4 cut(s) | 846, 1411, 1535, 1587 |
| BstX2I | RGATCY | 2 cut(s) | 606, 874 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1228 |
| BstYI | RGATCY | 2 cut(s) | 606, 874 |
| BstZ17I | GTATAC | 2 cut(s) | 440, 742 |
| Bsu15I | ATCGAT | 1 cut(s) | 1264 |
| BsuI | GTATCC | 4 cut(s) | 114, 127, 143, 208 |
| BsuRI | GGCC | 5 cut(s) | 252, 1053, 1220, 1319, 1497 |
| BsuTUI | ATCGAT | 1 cut(s) | 1264 |
| BtsCI | GGATG | 4 cut(s) | 384, 1100, 1241, 1357 |
| BtsI | GCAGTG | 1 cut(s) | 939 |
| BtsIMutI | CAGTG | 2 cut(s) | 628, 939 |
| BveI | ACCTGC | 1 cut(s) | 146 |
| Cac8I | GCNNGC | 4 cut(s) | 351, 702, 852, 930 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1282 |
| CfoI | GCGC | 1 cut(s) | 1507 |
| Cfr10I | RCCGGY | 1 cut(s) | 349 |
| ClaI | ATCGAT | 1 cut(s) | 1264 |
| CseI | GACGC | 1 cut(s) | 1388 |
| Csp6I | GTAC | 4 cut(s) | 469, 483, 1108, 1296 |
| CviAII | CATG | 3 cut(s) | 692, 844, 1457 |
| CviQI | GTAC | 4 cut(s) | 469, 483, 1108, 1296 |
| DdeI | CTNAG | 6 cut(s) | 106, 159, 276, 1013, 1275, 1331 |
| DpnI | GATC | 7 cut(s) | 209, 265, 594, 608, 819, 876, 906 |
| DpnII | GATC | 7 cut(s) | 207, 263, 592, 606, 817, 874, 904 |
| DraI | TTTAAA | 4 cut(s) | 490, 685, 835, 1444 |
| EaeI | YGGCCR | 2 cut(s) | 1051, 1218 |
| Eam1104I | CTCTTC | 4 cut(s) | 76, 545, 932, 1155 |
| EarI | CTCTTC | 4 cut(s) | 76, 545, 932, 1155 |
| Eco130I | CCWWGG | 3 cut(s) | 253, 878, 1221 |
| Eco147I | AGGCCT | 1 cut(s) | 1497 |
| Eco57I | CTGAAG | 2 cut(s) | 548, 1566 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 614, 977 |
| EcoRI | GAATTC | 1 cut(s) | 13 |
| EcoRII | CCWGG | 3 cut(s) | 55, 142, 704 |
| EcoT14I | CCWWGG | 3 cut(s) | 253, 878, 1221 |
| EcoT22I | ATGCAT | 2 cut(s) | 1243, 1458 |
| ErhI | CCWWGG | 3 cut(s) | 253, 878, 1221 |
| Esp3I | CGTCTC | 1 cut(s) | 636 |
| FaeI | CATG | 3 cut(s) | 695, 847, 1460 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 58, 90 |
| FatI | CATG | 3 cut(s) | 691, 843, 1456 |
| FauNDI | CATATG | 2 cut(s) | 499, 538 |
| FbaI | TGATCA | 1 cut(s) | 207 |
| FblI | GTMKAC | 2 cut(s) | 439, 741 |
| Fnu4HI | GCNGC | 3 cut(s) | 1028, 1320, 1590 |
| FokI | GGATG | 4 cut(s) | 371, 1087, 1228, 1364 |
| Fsp4HI | GCNGC | 3 cut(s) | 1028, 1320, 1590 |
| FspBI | CTAG | 2 cut(s) | 642, 1464 |
| GlaI | GCGC | 1 cut(s) | 1506 |
| GluI | GCNGC | 3 cut(s) | 1028, 1320, 1590 |
| GsuI | CTGGAG | 2 cut(s) | 78, 254 |
| HaeII | RGCGCY | 1 cut(s) | 1508 |
| HaeIII | GGCC | 5 cut(s) | 252, 1053, 1220, 1319, 1497 |
| HapII | CCGG | 2 cut(s) | 350, 1559 |
| HgaI | GACGC | 1 cut(s) | 1388 |
| HhaI | GCGC | 1 cut(s) | 1507 |
| Hin1II | CATG | 3 cut(s) | 695, 847, 1460 |
| Hin6I | GCGC | 1 cut(s) | 1505 |
| HinP1I | GCGC | 1 cut(s) | 1505 |
| HindIII | AAGCTT | 1 cut(s) | 758 |
| HinfI | GANTC | 7 cut(s) | 52, 316, 944, 1187, 1273, 1279, 1562 |
| HpaII | CCGG | 2 cut(s) | 350, 1559 |
| Hpy166II | GTNNAC | 6 cut(s) | 230, 440, 742, 1211, 1272, 1296 |
| Hpy188III | TCNNGA | 7 cut(s) | 21, 80, 374, 730, 980, 1157, 1283 |
| Hpy8I | GTNNAC | 6 cut(s) | 230, 440, 742, 1211, 1272, 1296 |
| Hpy99I | CGWCG | 1 cut(s) | 1404 |
| HpyAV | CCTTC | 6 cut(s) | 523, 593, 1015, 1133, 1166, 1339 |
| HpyCH4III | ACNGT | 3 cut(s) | 482, 746, 912 |
| HpyCH4IV | ACGT | 2 cut(s) | 1250, 1298 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 697, 1494, 1550 |
| HpyF3I | CTNAG | 6 cut(s) | 106, 159, 276, 1013, 1275, 1331 |
| HpySE526I | ACGT | 2 cut(s) | 1250, 1298 |
| Hsp92II | CATG | 3 cut(s) | 695, 847, 1460 |
| HspAI | GCGC | 1 cut(s) | 1505 |
| KroI | GCCGGC | 1 cut(s) | 349 |
| KroNI | GCCGGC | 1 cut(s) | 351 |
| Ksp22I | TGATCA | 1 cut(s) | 207 |
| Kzo9I | GATC | 7 cut(s) | 207, 263, 592, 606, 817, 874, 904 |
| LguI | GCTCTTC | 1 cut(s) | 76 |
| LmnI | GCTCC | 2 cut(s) | 847, 1024 |
| Lsp1109I | GCAGC | 2 cut(s) | 1039, 1601 |
| LweI | GCATC | 4 cut(s) | 352, 528, 1228, 1250 |
| MaeI | CTAG | 2 cut(s) | 642, 1464 |
| MaeII | ACGT | 2 cut(s) | 1250, 1298 |
| MaeIII | GTNAC | 1 cut(s) | 884 |
| MalI | GATC | 7 cut(s) | 209, 265, 594, 608, 819, 876, 906 |
| MboI | GATC | 7 cut(s) | 207, 263, 592, 606, 817, 874, 904 |
| MflI | RGATCY | 2 cut(s) | 606, 874 |
| MhlI | GDGCHC | 1 cut(s) | 246 |
| MlsI | TGGCCA | 2 cut(s) | 1053, 1220 |
| MluCI | AATT | 9 cut(s) | 13, 110, 197, 327, 780, 921, 984, 1057, 1198 |
| MluNI | TGGCCA | 2 cut(s) | 1053, 1220 |
| MlyI | GAGTC | 3 cut(s) | 953, 1267, 1571 |
| MmeI | TCCRAC | 1 cut(s) | 1149 |
| Mox20I | TGGCCA | 2 cut(s) | 1053, 1220 |
| Mph1103I | ATGCAT | 2 cut(s) | 1243, 1458 |
| MroNI | GCCGGC | 1 cut(s) | 349 |
| MroXI | GAANNNNTTC | 3 cut(s) | 51, 362, 674 |
| MscI | TGGCCA | 2 cut(s) | 1053, 1220 |
| MslI | CAYNNNNRTG | 1 cut(s) | 869 |
| Msp20I | TGGCCA | 2 cut(s) | 1053, 1220 |
| MspA1I | CMGCKG | 1 cut(s) | 598 |
| MspCI | CTTAAG | 1 cut(s) | 1499 |
| MspI | CCGG | 2 cut(s) | 350, 1559 |
| MspR9I | CCNGG | 3 cut(s) | 57, 144, 706 |
| Mva1269I | GAATGC | 2 cut(s) | 1338, 1458 |
| MvaI | CCWGG | 3 cut(s) | 57, 144, 706 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 697, 1494, 1550 |
| NaeI | GCCGGC | 1 cut(s) | 351 |
| NdeI | CATATG | 2 cut(s) | 499, 538 |
| NdeII | GATC | 7 cut(s) | 207, 263, 592, 606, 817, 874, 904 |
| NgoMIV | GCCGGC | 1 cut(s) | 349 |
| NlaIII | CATG | 3 cut(s) | 695, 847, 1460 |
| NlaIV | GGNNCC | 3 cut(s) | 7, 876, 996 |
| NsiI | ATGCAT | 2 cut(s) | 1243, 1458 |
| NspI | RCATGY | 1 cut(s) | 695 |
| PceI | AGGCCT | 1 cut(s) | 1497 |
| PciSI | GCTCTTC | 1 cut(s) | 76 |
| PctI | GAATGC | 2 cut(s) | 1338, 1458 |
| PdiI | GCCGGC | 1 cut(s) | 351 |
| PdmI | GAANNNNTTC | 3 cut(s) | 51, 362, 674 |
| PfeI | GAWTC | 4 cut(s) | 52, 316, 1187, 1279 |
| PfoI | TCCNGGA | 1 cut(s) | 55 |
| PkrI | GCNGC | 3 cut(s) | 1029, 1321, 1591 |
| PleI | GAGTC | 3 cut(s) | 952, 1267, 1570 |
| PpsI | GAGTC | 3 cut(s) | 952, 1267, 1570 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1561 |
| PshBI | ATTAAT | 1 cut(s) | 330 |
| PsiI | TTATAA | 1 cut(s) | 1203 |
| Psp6I | CCWGG | 3 cut(s) | 55, 142, 704 |
| PspGI | CCWGG | 3 cut(s) | 55, 142, 704 |
| PspN4I | GGNNCC | 3 cut(s) | 7, 876, 996 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1282 |
| PsuI | RGATCY | 2 cut(s) | 606, 874 |
| PvuII | CAGCTG | 1 cut(s) | 598 |
| RsaI | GTAC | 4 cut(s) | 470, 484, 1109, 1297 |
| RsaNI | GTAC | 4 cut(s) | 469, 483, 1108, 1296 |
| RseI | CAYNNNNRTG | 1 cut(s) | 869 |
| SapI | GCTCTTC | 1 cut(s) | 76 |
| SatI | GCNGC | 3 cut(s) | 1028, 1320, 1590 |
| Sau3AI | GATC | 7 cut(s) | 207, 263, 592, 606, 817, 874, 904 |
| SchI | GAGTC | 3 cut(s) | 953, 1267, 1571 |
| ScrFI | CCNGG | 3 cut(s) | 57, 144, 706 |
| SduI | GDGCHC | 1 cut(s) | 246 |
| SfaNI | GCATC | 4 cut(s) | 352, 528, 1228, 1250 |
| SfcI | CTRYAG | 1 cut(s) | 989 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 869 |
| SmlI | CTYRAG | 2 cut(s) | 898, 1499 |
| SmoI | CTYRAG | 2 cut(s) | 898, 1499 |
| Sse9I | AATT | 9 cut(s) | 13, 110, 197, 327, 780, 921, 984, 1057, 1198 |
| SseBI | AGGCCT | 1 cut(s) | 1497 |
| SsiI | CCGC | 2 cut(s) | 517, 1320 |
| SspMI | CTAG | 2 cut(s) | 642, 1464 |
| StuI | AGGCCT | 1 cut(s) | 1497 |
| StyD4I | CCNGG | 3 cut(s) | 55, 142, 704 |
| StyI | CCWWGG | 3 cut(s) | 253, 878, 1221 |
| TaaI | ACNGT | 3 cut(s) | 482, 746, 912 |
| TaiI | ACGT | 2 cut(s) | 1253, 1301 |
| TaqI | TCGA | 4 cut(s) | 11, 477, 1264, 1402 |
| TasI | AATT | 9 cut(s) | 13, 110, 197, 327, 780, 921, 984, 1057, 1198 |
| TatI | WGTACW | 2 cut(s) | 482, 1107 |
| TauI | GCSGC | 1 cut(s) | 1322 |
| TfiI | GAWTC | 4 cut(s) | 52, 316, 1187, 1279 |
| TscAI | CASTG | 2 cut(s) | 628, 939 |
| TseI | GCWGC | 2 cut(s) | 1027, 1589 |
| TspDTI | ATGAA | 3 cut(s) | 327, 355, 462 |
| TspGWI | ACGGA | 3 cut(s) | 42, 377, 937 |
| TspRI | CASTG | 2 cut(s) | 628, 939 |
| Vha464I | CTTAAG | 1 cut(s) | 1499 |
| VspI | ATTAAT | 1 cut(s) | 330 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 614, 977 |
| XapI | RAATTY | 3 cut(s) | 13, 780, 984 |
| XceI | RCATGY | 1 cut(s) | 695 |
| XmiI | GTMKAC | 2 cut(s) | 439, 741 |
| XmnI | GAANNNNTTC | 3 cut(s) | 51, 362, 674 |
| XspI | CTAG | 2 cut(s) | 642, 1464 |
| Zsp2I | ATGCAT | 2 cut(s) | 1243, 1458 |