FvH4_5g00310

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000183
Physical Location & Seq
Reverse (-)
215512 .. 223198
7687 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00310.t8

Sequence Viewer

Length: 2610 bp
ATGAAAACGAGTTACTTGTCCAAGCACGGAGGGTCATCAAATGCATGCACGCGTGAAGAGAATACATCGTACCACTTTGAAGTGAAACCAGAGTTTCTTAAGGGTGCTTTGACAAGATTTTCTCAGTTCTTTGTGTCACCTCTAATGAAAAGTGAAGCCATGGAGCGAGAGTTACAGTCTATAGATTCAGAGTTCAACCGGGTTCTGCAAAACGATTTTTGCCGCCTTGAACAACTTCAAGGTCATACAGCCACACCTGGTCACCCATTTAATAATTTCAGTCGGGGAAATAAGAGAAGCTTGGATGATGCAAGGGAAAAAGGGATAAACTTACGTCAACAAATAATGCAAATGTACACAGACTATTACCATGGCGGATTAATGAAGCTAGTTGTCATTGGTGGAGAATCTCTTGATATACTTGAGCAGTGGGTTTTGGAATTGTTTGGAGATGTCAAAAAAGGTCCCCAAGTTAACCTGGAGTTCAAAGCTGAAGGTCCTATTTGGAATGTTGGAAAACTTTACAGGCTAGAGGCCGTTAATGATGTTCATATACTCCACTTAGCATGGGCACTTCCAAGTCTTCATCAACATTATTTGAAATGCCCAGATTATTTTTTAAGCCATCTTCTTGAGCATGAGGGCAGGGGAAGTCTGTATTTTTATCTCAAAGCTAAAGGATGGGCAACATATCTCAATGCTTCGGTGAACCGCTATTCTGTGGCTGATGTCTTTTGCATGATCATATACCTTACTGACTCTGGATTGGAGCAGATTTTTGAGATAGTTGGCTCGGTGTATCAATACATTAAATTATTGCGTCAAGTATCACCTCAAGAATGGATATTCCGGGAACTCCAGGATATTGGGAACATGAGTTTTAGATTTATGCAGGATCAGCCCCAGGATGATTATGCTGTAAAACTTGGAGGAAATTTATTACGCTATTCAGCAGAACATGTTATTTATGGGGACTATGTGTACGAGACTTGGGATAAGGAATTGATAGAATATGTTCTTGGCTTCTTCAGACCAGAAAACATGAGGATTGATGTGATATCAAAGTCCTCATTCAAGTTGGAAAATTTCCAGTGCGAGCCTTGGTTTGGTTCACATTATACTGAAGAAGATATATCTCCATCTTTGATGAATTTGTGGAAGGATCCTCCAGAAGTTGATAATTCATTGCATCTCCCTCTAAAGAATATGTTCATTCCTGGTGATTTCTCCATCCGTTCAGATGTCTGTTGTCCTGATAGTGCAAATATATCTTCTCCAAGATGTATACTTGATGAACCATTGGTGAAGATCTGGTACAAGCTTGACAGTACTTTTAAACAACCACGGGTAAATGCATACTTCCGCATAAATCTGAAGGGTGGATATGATAATGTGAAGAGTTATGTTTTGACTGAACTGTATATTGAGCTTCTTGAGGATGAGCTGAATGAAATAGTATATCAGGCCAATGTTGCCGGGCTGGGAACTCATGTAAATGGATCTAACGACTTTATGGAGTTGAAGGTTTACGGTTTCAACGATAAGCTTCCAGCTTTATTGTCAAAAATTCTGACAGTGACCAGAAACTTCTTGCCAACATATGATCGTTTCGAGGTTATCAGAGAAAGAATGGAGAGAATGTTAAAGAACACTAATATGGATCCTCAGAGTCACTCTTCATATTTGAGACGTCAAGTTTTGTACCATAAATATTATGATGTAGATGAAGAGTTGCAAGTTTTGAACAGTTTGTCTGTTTCTGATATGAAGTTGTTTATTCCCGAGCTTTGTTCCCAGCTTTACATTGAAGGCTTTTGTCATGGAAATCTGGTAGAAGAAGAGGCACTTCACCTTTCCAATATATTTAAAACCAATTTTACTGTGGAACCACTTCCTATCGAATTGAGGCATAAGGATCATTGTATGTGCCTTCCTCCTTCTGCTAACCTCGTTAGAGAAGCCTGTGTGAAGAATAATTCTGAAACAAACTCTGTCATTGAGTTATATTTTCAAATTGAGATCGAGTCCACCAGATTGAGAGCATTGGCAAAACTTTTTATGAAAATTGTAGACGAACCACTTTACGATCAGCTAAGGACGAAGGAGCAGCTTGGTTATGTTGTTTGGTGTGGCCTGCGAGAAATATGTCGTGTATATGGCTTTGTTTTCTGTGTTGAGTCGTCTGAGCACAACCCAATCTACTTGCAAGGGAGAGTTGACAACTTTATAAATGGTGTGGATCTATTGTTGCACGGACTGGATGATGATTCCTTTGAGAATTATAAAGATGGACTAATAGCAAATCTATTGGAAAGAGATGAAACACTCGGACATGAAACGTCTAGGCTTTGGGAGGAAATTACCAATAAAAGGTATACATATGACTGGCCAAAAAGGGTAGCTGAAGAGGTCAGAAGCCTTCAGAAGGAGGACATTATTAACTTCTACAAAACCTACTTGCAACCATCATCTCCCAAGTGTCGGAGACTTGCAATTCGTGTTTGGGGTTGCAACTCTGATTTGAAAGAAGCTGAAGCACCACCGGAGTCTATACAACTCATTCAGGACCTTGCAGCCTTTAAGATGTCATCTGAATTCTATCTTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

870

Amino Acids

101.17

Weight (kDa)

5.32

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16_M PF16187 48 - 333 7.4e-85 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 338 - 519 7e-10 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 439 - 538 1.2e-24 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2228, 2283
AatII GACGTC 1 cut(s) 1693
AccI GTMKAC 3 cut(s) 1285, 2070, 2375
AccII CGCG 1 cut(s) 52
AciI CCGC 4 cut(s) 223, 375, 712, 1363
AclWI GGATC 8 cut(s) 903, 1157, 1170, 1507, 1655, 1668, 1923, 2247
AcoI YGGCCR 1 cut(s) 2387
AcsI RAATTY 5 cut(s) 934, 1084, 1150, 1566, 2594
AcuI CTGAAG 7 cut(s) 513, 1012, 1143, 1394, 2405, 2424, 2553
AcyI GRCGYC 1 cut(s) 1690
AfaI GTAC 6 cut(s) 71, 356, 983, 1316, 1330, 1703
AfiI CCNNNNNNNGG 4 cut(s) 1106, 2370, 2425, 2481
AflII CTTAAG 1 cut(s) 98
AflIII ACRYGT 2 cut(s) 50, 958
AjnI CCWGG 5 cut(s) 256, 477, 858, 903, 1216
AjuI GAANNNNNNNTTGG 3 cut(s) 1002, 1034, 2586
Alw21I GWGCWC 1 cut(s) 2190
Alw26I GTCTC 3 cut(s) 980, 1681, 2479
AlwI GGATC 8 cut(s) 903, 1157, 1170, 1507, 1655, 1668, 1923, 2247
Ama87I CYCGRG 1 cut(s) 1781
AoxI GGCC 4 cut(s) 534, 1464, 2131, 2387
ApeKI GCWGC 2 cut(s) 2107, 2573
ApoI RAATTY 5 cut(s) 934, 1084, 1150, 1566, 2594
ArsI GACNNNNNNTTYG 2 cut(s) 2478, 2510
AseI ATTAAT 1 cut(s) 380
Asp700I GAANNNNTTC 4 cut(s) 234, 1014, 1208, 1890
AspS9I GGNCC 3 cut(s) 464, 497, 2566
AsuC2I CCSGG 3 cut(s) 200, 851, 1477
AsuHPI GGTGA 7 cut(s) 129, 254, 718, 822, 1232, 1315, 1841
AvaI CYCGRG 1 cut(s) 1781
AvaII GGWCC 3 cut(s) 464, 497, 2566
BaeGI GKGCMC 1 cut(s) 574
BalI TGGCCA 1 cut(s) 2389
BamHI GGATCC 2 cut(s) 1162, 1660
BarI GAAGNNNNNNTAC 2 cut(s) 1298, 1330
BbsI GAAGAC 1 cut(s) 575
Bbv12I GWGCWC 1 cut(s) 2190
BbvI GCAGC 2 cut(s) 2119, 2585
BccI CCATC 6 cut(s) 633, 675, 1147, 1238, 2282, 2473
BceAI ACGGC 1 cut(s) 521
BciT130I CCWGG 5 cut(s) 258, 479, 860, 905, 1218
BclI TGATCA 1 cut(s) 741
BcnI CCSGG 3 cut(s) 200, 851, 1477
BcoDI GTCTC 3 cut(s) 980, 1681, 2479
BfaI CTAG 3 cut(s) 389, 530, 2343
BfmI CTRYAG 1 cut(s) 180
BfrI CTTAAG 1 cut(s) 98
BglII AGATCT 1 cut(s) 1308
BisI GCNGC 3 cut(s) 223, 2108, 2574
BlsI GCNGC 3 cut(s) 224, 2109, 2575
BmcAI AGTACT 1 cut(s) 1330
Bme1390I CCNGG 8 cut(s) 200, 258, 479, 851, 860, 905, 1218, 1477
Bme18I GGWCC 3 cut(s) 464, 497, 2566
BmeT110I CYCGRG 1 cut(s) 1781
BmgT120I GGNCC 3 cut(s) 464, 497, 2566
BmiI GGNNCC 4 cut(s) 466, 1164, 1662, 1887
BmrFI CCNGG 8 cut(s) 200, 258, 479, 851, 860, 905, 1218, 1477
BmsI GCATC 2 cut(s) 298, 1198
BpiI GAAGAC 1 cut(s) 575
BpmI CTGGAG 3 cut(s) 500, 842, 1152
Bpu10I CCTNAGC 1 cut(s) 2093
BpuEI CTTGAG 4 cut(s) 443, 653, 819, 1454
BpuMI CCSGG 3 cut(s) 200, 851, 1477
BsaHI GRCGYC 1 cut(s) 1690
BsaJI CCNNGG 5 cut(s) 159, 370, 903, 1100, 1343
BsaWI WCCGGW 1 cut(s) 2542
BsaXI ACNNNNNCTCC 4 cut(s) 155, 185, 1625, 1655
Bsc4I CCNNNNNNNGG 4 cut(s) 1106, 2370, 2425, 2481
Bse1I ACTGG 3 cut(s) 1090, 2262, 2390
Bse3DI GCAATG 1 cut(s) 1184
BseBI CCWGG 5 cut(s) 258, 479, 860, 905, 1218
BseDI CCNNGG 5 cut(s) 159, 370, 903, 1100, 1343
BseGI GGATG 6 cut(s) 310, 686, 913, 1230, 1444, 2266
BseLI CCNNNNNNNGG 4 cut(s) 1106, 2370, 2425, 2481
BseMI GCAATG 1 cut(s) 1184
BseMII CTCAG 3 cut(s) 137, 1679, 2175
BseNI ACTGG 3 cut(s) 1090, 2262, 2390
BseSI GKGCMC 1 cut(s) 574
BseXI GCAGC 2 cut(s) 2119, 2585
BseYI CCCAGC 2 cut(s) 1480, 1794
Bsh1236I CGCG 1 cut(s) 52
BshFI GGCC 4 cut(s) 536, 1466, 2133, 2389
BsiHKAI GWGCWC 1 cut(s) 2190
BsiHKCI CYCGRG 1 cut(s) 1781
BsiSI CCGG 4 cut(s) 199, 850, 1476, 2543
BslFI GGGAC 2 cut(s) 450, 986
BslI CCNNNNNNNGG 4 cut(s) 1106, 2370, 2425, 2481
BsmAI GTCTC 3 cut(s) 980, 1681, 2479
BsmBI CGTCTC 1 cut(s) 1681
BsmFI GGGAC 2 cut(s) 450, 986
BsnI GGCC 4 cut(s) 536, 1466, 2133, 2389
BsoBI CYCGRG 1 cut(s) 1781
Bsp1286I GDGCHC 2 cut(s) 574, 2190
Bsp1407I TGTACA 1 cut(s) 354
Bsp19I CCATGG 2 cut(s) 159, 370
BspACI CCGC 4 cut(s) 223, 375, 712, 1363
BspANI GGCC 4 cut(s) 536, 1466, 2133, 2389
BspCNI CTCAG 3 cut(s) 136, 1678, 2176
BspFNI CGCG 1 cut(s) 52
BspLI GGNNCC 4 cut(s) 466, 1164, 1662, 1887
BspPI GGATC 8 cut(s) 903, 1157, 1170, 1507, 1655, 1668, 1923, 2247
BspTI CTTAAG 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 1184
BsrGI TGTACA 1 cut(s) 354
BsrI ACTGG 3 cut(s) 1090, 2262, 2390
BssECI CCNNGG 5 cut(s) 159, 370, 903, 1100, 1343
BssNAI GTATAC 2 cut(s) 1286, 2376
BssNI GRCGYC 1 cut(s) 1690
BssT1I CCWWGG 3 cut(s) 159, 370, 1100
Bst1107I GTATAC 2 cut(s) 1286, 2376
Bst2UI CCWGG 5 cut(s) 258, 479, 860, 905, 1218
Bst4CI ACNGT 7 cut(s) 177, 1328, 1419, 1532, 1576, 1748, 1882
Bst6I CTCTTC 6 cut(s) 51, 1391, 1681, 1722, 1833, 2400
BstACI GRCGYC 1 cut(s) 1690
BstAFI CTTAAG 1 cut(s) 98
BstAUI TGTACA 1 cut(s) 354
BstC8I GCNNGC 4 cut(s) 46, 50, 1097, 2135
BstDEI CTNAG 5 cut(s) 123, 562, 1665, 2093, 2184
BstDSI CCRYGG 3 cut(s) 159, 370, 1343
BstEII GGTNACC 1 cut(s) 260
BstENI CCTNNNNNAGG 1 cut(s) 2423
BstF5I GGATG 6 cut(s) 310, 686, 913, 1230, 1444, 2266
BstFNI CGCG 1 cut(s) 52
BstMAI GTCTC 3 cut(s) 980, 1681, 2479
BstMWI GCNNNNNNNGC 2 cut(s) 898, 1472
BstNI CCWGG 5 cut(s) 258, 479, 860, 905, 1218
BstNSI RCATGY 2 cut(s) 48, 962
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 8 cut(s) 198, 256, 477, 849, 858, 903, 1216, 1475
BstSFI CTRYAG 1 cut(s) 180
BstSLI GKGCMC 1 cut(s) 574
BstUI CGCG 1 cut(s) 52
BstV1I GCAGC 2 cut(s) 2119, 2585
BstV2I GAAGAC 1 cut(s) 575
BstX2I RGATCY 5 cut(s) 1162, 1308, 1499, 1660, 2239
BstXI CCANNNNNNTGG 1 cut(s) 866
BstYI RGATCY 5 cut(s) 1162, 1308, 1499, 1660, 2239
BstZ17I GTATAC 2 cut(s) 1286, 2376
BsuRI GGCC 4 cut(s) 536, 1466, 2133, 2389
BtgI CCRYGG 3 cut(s) 159, 370, 1343
BtsCI GGATG 6 cut(s) 310, 686, 913, 1230, 1444, 2266
BtsI GCAGTG 1 cut(s) 434
BtsIMutI CAGTG 3 cut(s) 434, 1097, 1581
Cac8I GCNNGC 4 cut(s) 46, 50, 1097, 2135
Cfr13I GGNCC 3 cut(s) 464, 497, 2566
CseI GACGC 1 cut(s) 809
CsiI ACCWGGT 1 cut(s) 256
Csp6I GTAC 6 cut(s) 70, 355, 982, 1315, 1329, 1702
CviQI GTAC 6 cut(s) 70, 355, 982, 1315, 1329, 1702
DdeI CTNAG 5 cut(s) 123, 562, 1665, 2093, 2184
DraI TTTAAA 2 cut(s) 1336, 1867
EaeI YGGCCR 1 cut(s) 2387
Eam1104I CTCTTC 6 cut(s) 51, 1391, 1681, 1722, 1833, 2400
EarI CTCTTC 6 cut(s) 51, 1391, 1681, 1722, 1833, 2400
EciI GGCGGA 1 cut(s) 390
Eco130I CCWWGG 3 cut(s) 159, 370, 1100
Eco32I GATATC 1 cut(s) 1059
Eco47I GGWCC 3 cut(s) 464, 497, 2566
Eco57I CTGAAG 7 cut(s) 513, 1012, 1143, 1394, 2405, 2424, 2553
Eco88I CYCGRG 1 cut(s) 1781
Eco91I GGTNACC 1 cut(s) 260
EcoNI CCTNNNNNAGG 1 cut(s) 2423
EcoO109I RGGNCCY 3 cut(s) 464, 497, 2566
EcoO65I GGTNACC 1 cut(s) 260
EcoRI GAATTC 1 cut(s) 2594
EcoRII CCWGG 5 cut(s) 256, 477, 858, 903, 1216
EcoRV GATATC 1 cut(s) 1059
EcoT14I CCWWGG 3 cut(s) 159, 370, 1100
EcoT22I ATGCAT 2 cut(s) 46, 1357
ErhI CCWWGG 3 cut(s) 159, 370, 1100
Esp3I CGTCTC 1 cut(s) 1681
FalI AAGNNNNNCTT 4 cut(s) 284, 316, 1830, 1862
FaqI GGGAC 2 cut(s) 450, 986
FauNDI CATATG 2 cut(s) 1600, 2380
FbaI TGATCA 1 cut(s) 741
FblI GTMKAC 3 cut(s) 1285, 2070, 2375
Fnu4HI GCNGC 3 cut(s) 223, 2108, 2574
FokI GGATG 6 cut(s) 317, 693, 920, 1217, 1451, 2273
Fsp4HI GCNGC 3 cut(s) 223, 2108, 2574
FspBI CTAG 3 cut(s) 389, 530, 2343
GluI GCNGC 3 cut(s) 223, 2108, 2574
GsaI CCCAGC 2 cut(s) 1484, 1798
GsuI CTGGAG 3 cut(s) 500, 842, 1152
HaeIII GGCC 4 cut(s) 536, 1466, 2133, 2389
HapII CCGG 4 cut(s) 199, 850, 1476, 2543
HgaI GACGC 1 cut(s) 809
Hin1I GRCGYC 1 cut(s) 1690
HincII GTYRAC 3 cut(s) 338, 475, 2218
HindII GTYRAC 3 cut(s) 338, 475, 2218
HindIII AAGCTT 3 cut(s) 298, 1319, 1544
HinfI GANTC 8 cut(s) 185, 407, 758, 1669, 2024, 2177, 2267, 2546
HpaI GTTAAC 1 cut(s) 475
HpaII CCGG 4 cut(s) 199, 850, 1476, 2543
HphI GGTGA 7 cut(s) 129, 254, 718, 822, 1232, 1315, 1841
Hpy188III TCNNGA 9 cut(s) 413, 632, 762, 836, 1169, 1253, 1433, 1781, 2564
HpyCH4III ACNGT 7 cut(s) 177, 1328, 1419, 1532, 1576, 1748, 1882
HpyCH4IV ACGT 3 cut(s) 334, 1690, 2339
HpyF10VI GCNNNNNNNGC 2 cut(s) 898, 1472
HpyF3I CTNAG 5 cut(s) 123, 562, 1665, 2093, 2184
HpySE526I ACGT 3 cut(s) 334, 1690, 2339
Hsp92I GRCGYC 1 cut(s) 1690
Ksp22I TGATCA 1 cut(s) 741
KspAI GTTAAC 1 cut(s) 475
LmnI GCTCC 3 cut(s) 163, 769, 2104
Lsp1109I GCAGC 2 cut(s) 2119, 2585
LweI GCATC 2 cut(s) 298, 1198
MabI ACCWGGT 1 cut(s) 256
MaeI CTAG 3 cut(s) 389, 530, 2343
MaeII ACGT 3 cut(s) 334, 1690, 2339
MaeIII GTNAC 6 cut(s) 11, 135, 171, 260, 1576, 1670
MflI RGATCY 5 cut(s) 1162, 1308, 1499, 1660, 2239
MhlI GDGCHC 2 cut(s) 574, 2190
MlsI TGGCCA 1 cut(s) 2389
MluI ACGCGT 1 cut(s) 50
MluNI TGGCCA 1 cut(s) 2389
MlyI GAGTC 5 cut(s) 752, 1678, 2033, 2186, 2555
MmeI TCCRAC 3 cut(s) 493, 1059, 2462
Mox20I TGGCCA 1 cut(s) 2389
Mph1103I ATGCAT 2 cut(s) 46, 1357
MroXI GAANNNNTTC 4 cut(s) 234, 1014, 1208, 1890
MscI TGGCCA 1 cut(s) 2389
MslI CAYNNNNRTG 2 cut(s) 1494, 1655
Msp20I TGGCCA 1 cut(s) 2389
MspCI CTTAAG 1 cut(s) 98
MspI CCGG 4 cut(s) 199, 850, 1476, 2543
MspR9I CCNGG 8 cut(s) 200, 258, 479, 851, 860, 905, 1218, 1477
MvaI CCWGG 5 cut(s) 258, 479, 860, 905, 1218
MvnI CGCG 1 cut(s) 52
MwoI GCNNNNNNNGC 2 cut(s) 898, 1472
NciI CCSGG 3 cut(s) 200, 851, 1477
NcoI CCATGG 2 cut(s) 159, 370
NdeI CATATG 2 cut(s) 1600, 2380
NlaIV GGNNCC 4 cut(s) 466, 1164, 1662, 1887
NmuCI GTSAC 4 cut(s) 135, 260, 1576, 1670
NsiI ATGCAT 2 cut(s) 46, 1357
NspI RCATGY 2 cut(s) 48, 962
PaeI GCATGC 1 cut(s) 48
PciI ACATGT 1 cut(s) 958
PcsI WCGNNNNNNNCGW 1 cut(s) 1536
PdmI GAANNNNTTC 4 cut(s) 234, 1014, 1208, 1890
PfeI GAWTC 3 cut(s) 185, 407, 2267
PfoI TCCNGGA 2 cut(s) 849, 858
PkrI GCNGC 3 cut(s) 224, 2109, 2575
PleI GAGTC 5 cut(s) 752, 1677, 2032, 2185, 2554
PpsI GAGTC 5 cut(s) 752, 1677, 2032, 2185, 2554
PpuMI RGGWCCY 3 cut(s) 464, 497, 2566
PscI ACATGT 1 cut(s) 958
PshBI ATTAAT 1 cut(s) 380
PsiI TTATAA 2 cut(s) 2228, 2283
Psp5II RGGWCCY 3 cut(s) 464, 497, 2566
Psp6I CCWGG 5 cut(s) 256, 477, 858, 903, 1216
PspEI GGTNACC 1 cut(s) 260
PspFI CCCAGC 2 cut(s) 1480, 1794
PspGI CCWGG 5 cut(s) 256, 477, 858, 903, 1216
PspN4I GGNNCC 4 cut(s) 466, 1164, 1662, 1887
PspPI GGNCC 3 cut(s) 464, 497, 2566
PspPPI RGGWCCY 3 cut(s) 464, 497, 2566
PsuI RGATCY 5 cut(s) 1162, 1308, 1499, 1660, 2239
RsaI GTAC 6 cut(s) 71, 356, 983, 1316, 1330, 1703
RsaNI GTAC 6 cut(s) 70, 355, 982, 1315, 1329, 1702
RseI CAYNNNNRTG 2 cut(s) 1494, 1655
SatI GCNGC 3 cut(s) 223, 2108, 2574
Sau96I GGNCC 3 cut(s) 464, 497, 2566
ScaI AGTACT 1 cut(s) 1330
SchI GAGTC 5 cut(s) 752, 1678, 2033, 2186, 2555
ScrFI CCNGG 8 cut(s) 200, 258, 479, 851, 860, 905, 1218, 1477
SduI GDGCHC 2 cut(s) 574, 2190
SexAI ACCWGGT 1 cut(s) 256
SfaNI GCATC 2 cut(s) 298, 1198
SfcI CTRYAG 1 cut(s) 180
SinI GGWCC 3 cut(s) 464, 497, 2566
SmiMI CAYNNNNRTG 2 cut(s) 1494, 1655
SmlI CTYRAG 5 cut(s) 98, 422, 632, 834, 1433
SmoI CTYRAG 5 cut(s) 98, 422, 632, 834, 1433
SphI GCATGC 1 cut(s) 48
SsiI CCGC 4 cut(s) 223, 375, 712, 1363
SspI AATATT 1 cut(s) 1712
SspMI CTAG 3 cut(s) 389, 530, 2343
StyD4I CCNGG 8 cut(s) 198, 256, 477, 849, 858, 903, 1216, 1475
StyI CCWWGG 3 cut(s) 159, 370, 1100
TaaI ACNGT 7 cut(s) 177, 1328, 1419, 1532, 1576, 1748, 1882
TaiI ACGT 3 cut(s) 337, 1693, 2342
TaqI TCGA 3 cut(s) 1611, 1899, 2022
TatI WGTACW 2 cut(s) 354, 1328
TauI GCSGC 1 cut(s) 225
TfiI GAWTC 3 cut(s) 185, 407, 2267
TscAI CASTG 3 cut(s) 434, 1097, 1581
TseFI GTSAC 4 cut(s) 135, 260, 1576, 1670
TseI GCWGC 2 cut(s) 2107, 2573
Tsp45I GTSAC 4 cut(s) 135, 260, 1576, 1670
TspGWI ACGGA 3 cut(s) 42, 1223, 2268
TspRI CASTG 3 cut(s) 434, 1097, 1581
Vha464I CTTAAG 1 cut(s) 98
VpaK11BI GGWCC 3 cut(s) 464, 497, 2566
VspI ATTAAT 1 cut(s) 380
XagI CCTNNNNNAGG 1 cut(s) 2423
XapI RAATTY 5 cut(s) 934, 1084, 1150, 1566, 2594
XceI RCATGY 2 cut(s) 48, 962
XcmI CCANNNNNNNNNTGG 1 cut(s) 1879
XmiI GTMKAC 3 cut(s) 1285, 2070, 2375
XmnI GAANNNNTTC 4 cut(s) 234, 1014, 1208, 1890
XspI CTAG 3 cut(s) 389, 530, 2343
ZraI GACGTC 1 cut(s) 1691
ZrmI AGTACT 1 cut(s) 1330
Zsp2I ATGCAT 2 cut(s) 46, 1357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.