RLG00000036353

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000183
Physical Location & Seq
Reverse (-)
79589022 .. 79608197
19176 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036353

Sequence Viewer

Length: 2304 bp
ATGCAATCTTGGGAAGTGGGAGGAGTTTTGAGGTCTATTTTCGCACGAGAGAAGGGGCAAAAAAAACACATTGAGCCTGAGCTTTCTTGGAATACAAGCAACCATCCTTGGGCTGAAGCAAGGCAAGTTGCTCCATCCGACGATATCGTAATAAAGTCTCCAGATGATAATCGACTCTACAGATTAATCCAGCTCGAGAATGGCCTCACTGCATTGCTCATTCACGACCCTGAGACTTACCTAGACGTTAAAACCATGAAAAAGGGGACTACTTCTCAGAACAAGGCAGCAGCCGCAATATGCGTCGGAATAGGCAGCTTCTCTGACCCTTTGGAGGCACAGGGGCTTGCACACTTTCTTGAACACATGCTCTTCATGGGGAGCACAGAGTTTCCAGATGAAAATGAGTATTGTAGTTACTTGTCCGAGCATGGTGGGTGTCGAAATGCATACACAGAATTTGAGCATACTTGCTACCATTTTGAAGTAAAGGCGGAGTTTCTTAAGGGTGCCTTGGCAAGGTTTTCTCAGTTCTTTGTTTCATCTCTATTGAGGATTGAATCCATGGAGAAAGAGTTACGGACTATAGATTCAGAGTTTAACGGGGTTCGGCAACTGGATGATTATCGCCTTTTTCAACTTCAATGCCATGCATCTTCAACTGGTCACCCATATAATAGATTCGCTTGTGGAAATAAGAAGAGCTTGGTTTCTGCGGTGGAAAAGGGGATCAACTTGCGGGAAAAAATAGTGCAATTGTACAGAGATTATTACCATGGTGGATTAATGAAGCTAGTGGTCATTGGTGGAGAATCTCTTGATGTACTTGAGCAGTGGGTTTTGGAATTGTATGGTAATATTAAAAAAGGTCCCCAAGTAAACCTGGAGTTCAAGGCAGAAGGTCCTATTTGGAAAGCTGGAAAACTCTACAGGTTGGAGGCTGTTGAAGATGTTCATATGCTCAGCTTAGCATGGACACTTCCATGTCTTTGTCAATACTATTTGAAGAAACCAGAATATTTTCCAGAGGGCAGGGGAAATTTGTATTTCTACCTCAAGGCTAGAGGGTGGGTAACACGTCTGAATGCTTATCATGACAGGAACGGCTCTTCCGTGGCTTATGTCTTTCGCATGATCATATACCTTACTAACTCTGGATTGGAGAAGTTTGTGTATCAATACATAAAGTTATTACGTCAAGCTTCACCACAAGAATGGATATTTAAGGAACTCCAGGTTATTGGGAACATGGATTTTAATTTGTTTGCGGGACGGCCCCAGGATGAATATGCTTCAGAACTTGCAGATTTCCAGTGTGAGCCTTGGTTTGGGTCACATTATACCGAGGAAGATATATCTTCATCTTTGATGGATTTGTGGAGGGATCCTCCGGAAATTGATGCTTCATTGCATCTGCCAGCAAAGAATGAGTTCATTCCTTCTGATTTTTCCATCTGTTCTGATACCCCATGTCTTAATGCTGCAAATACATCTTCTCCAAGATGTATACTACATGAACCATTAGTGAAGCTCTGGTACAAGCTCCACCGTACTCCTAAAGTTCGATGGGTAAATACATACTTCATCATCAATCTAAAGGGCGGATATGATGATGTGAAGAGTAGTGCCCGTGTTGCCAAACTGGAAACTTCTTTATCTATGTCGAAGGACAAACTGCAGCTAGAGTTCGAAGGTTTCAGTGATAAGATTCCAATTCTGTTGTCAAAAGTTTTGGAAACAGTCAAAATTTCTTGCCAGCTCATGATTGTTTTCAGACAACAAGTTTTGTTGCAGAATTTCTACGATGTTGATGAGAAGTTGCATATTTTGAATGGATTATCTGTTTCTGATTTGAAGTCATTTATTCCCGAGCTTTTTTCCCAGGGCTTTTGCCATGGCAATTTGTCAGAAGAAGAAGCAATTAGCCTTTCATCTGTAATTAAAACAACTTTCTCTGTACCACCACTCCCTACCGCATTGATGTATAGAGATTATTGTGTTTGCCTTCCTCCTGCTGCTGACCTCATTAGAGATGCTTCTGTCAAAAATAAGTCGGAAACTAACTCTGTGATTTTGCTGTATTTTCAAATTAAGCAACCAGTTGGGAGTGAGTCAACCAGACTGCCAGCATTGGTAGATCTTTTTCATACCATTGTAAAGGAACCACTTTTCAATCAGCTAAGGACAATGGAGCAACTCGGGTATGTTGTTCGGTGTGCCCCAGATCACCACTCATGTGGTGTTCTTGGCTTTTCTTTCTGTATTCAGTCGTCTAAGCCCAATCTATCTACAAGGGAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

768

Amino Acids

87.53

Weight (kDa)

6.03

Isoelectric Point (pI)

35.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 90 - 211 7.2e-29 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 247 - 411 3.7e-09 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 437 - 542 2.1e-19 Middle or third domain of peptidase_M16
Peptidase_M16_M PF16187 541 - 582 6.8e-08 Middle or third domain of peptidase_M16
PqqF-like_C_4 PF22456 715 - 759 1.5e-07 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 509
AccI GTMKAC 1 cut(s) 1507
AccIII TCCGGA 1 cut(s) 1390
AciI CCGC 7 cut(s) 294, 494, 716, 739, 1268, 1602, 1974
AclWI GGATC 3 cut(s) 737, 1379, 1392
AcsI RAATTY 4 cut(s) 458, 1039, 1746, 1795
AcuI CTGAAG 2 cut(s) 135, 1278
AfaI GTAC 5 cut(s) 761, 825, 1538, 1552, 1959
AfiI CCNNNNNNNGG 4 cut(s) 109, 334, 519, 1328
AflII CTTAAG 1 cut(s) 503
AflIII ACRYGT 1 cut(s) 1076
AjiI CACGTC 1 cut(s) 1079
AjnI CCWGG 4 cut(s) 882, 1233, 1278, 1881
AleI CACNNNNGTG 1 cut(s) 2235
AloI GAACNNNNNNTCC 2 cut(s) 1094, 1126
Alw21I GWGCWC 1 cut(s) 386
Alw26I GTCTC 3 cut(s) 162, 227, 2291
AlwI GGATC 3 cut(s) 737, 1379, 1392
Ama87I CYCGRG 3 cut(s) 194, 1868, 2198
Aor13HI TCCGGA 1 cut(s) 1390
AoxI GGCC 2 cut(s) 202, 1274
ApeKI GCWGC 6 cut(s) 287, 290, 315, 1481, 1678, 2015
ApoI RAATTY 4 cut(s) 458, 1039, 1746, 1795
AseI ATTAAT 2 cut(s) 185, 785
Asp700I GAANNNNTTC 3 cut(s) 951, 1020, 1430
AspS9I GGNCC 3 cut(s) 869, 902, 1275
AsuHPI GGTGA 3 cut(s) 659, 1197, 2219
AsuII TTCGAA 1 cut(s) 1689
AvaI CYCGRG 3 cut(s) 194, 1868, 2198
AvaII GGWCC 2 cut(s) 869, 902
BaeGI GKGCMC 2 cut(s) 1630, 2221
BamHI GGATCC 1 cut(s) 1384
BanI GGYRCC 1 cut(s) 509
BarI GAAGNNNNNNTAC 2 cut(s) 1520, 1552
BauI CACGAG 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 386
BbvI GCAGC 6 cut(s) 299, 302, 327, 1468, 1690, 2002
BccI CCATC 5 cut(s) 111, 142, 1363, 1460, 1560
BceAI ACGGC 2 cut(s) 1120, 1289
BciT130I CCWGG 4 cut(s) 884, 1235, 1280, 1883
BclI TGATCA 1 cut(s) 1134
BcoDI GTCTC 3 cut(s) 162, 227, 2291
BfaI CTAG 4 cut(s) 242, 794, 1062, 1682
BfmI CTRYAG 4 cut(s) 178, 585, 928, 1676
BfrI CTTAAG 1 cut(s) 503
BglII AGATCT 1 cut(s) 2137
BisI GCNGC 7 cut(s) 288, 291, 294, 316, 1482, 1679, 2016
BlpI GCTNAGC 2 cut(s) 962, 967
BlsI GCNGC 7 cut(s) 289, 292, 295, 317, 1483, 1680, 2017
Bme1390I CCNGG 4 cut(s) 884, 1235, 1280, 1883
Bme18I GGWCC 2 cut(s) 869, 902
BmeT110I CYCGRG 3 cut(s) 194, 1868, 2198
BmgBI CACGTC 1 cut(s) 1079
BmgT120I GGNCC 3 cut(s) 869, 902, 1275
BmiI GGNNCC 5 cut(s) 511, 871, 1277, 1386, 2163
BmrFI CCNGG 4 cut(s) 884, 1235, 1280, 1883
BmsI GCATC 4 cut(s) 662, 1390, 1420, 2023
BplI GAGNNNNNCTC 2 cut(s) 1372, 1404
BpmI CTGGAG 3 cut(s) 144, 905, 1217
Bpu10I CCTNAGC 2 cut(s) 78, 2180
Bpu1102I GCTNAGC 2 cut(s) 962, 967
Bpu14I TTCGAA 1 cut(s) 1689
BpuEI CTTGAG 2 cut(s) 848, 1040
BsaBI GATNNNNATC 2 cut(s) 168, 624
BsaWI WCCGGW 1 cut(s) 1390
BsaXI ACNNNNNCTCC 6 cut(s) 560, 590, 1480, 1510, 1950, 1980
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 334, 519, 1328
Bse1I ACTGG 5 cut(s) 621, 667, 1312, 1647, 2099
Bse3DI GCAATG 2 cut(s) 212, 1406
Bse8I GATNNNNATC 2 cut(s) 168, 624
BseAI TCCGGA 1 cut(s) 1390
BseBI CCWGG 4 cut(s) 884, 1235, 1280, 1883
BseGI GGATG 4 cut(s) 103, 134, 625, 1288
BseJI GATNNNNATC 2 cut(s) 168, 624
BseLI CCNNNNNNNGG 4 cut(s) 109, 334, 519, 1328
BseMI GCAATG 2 cut(s) 212, 1406
BseMII CTCAG 5 cut(s) 69, 222, 290, 542, 976
BseNI ACTGG 5 cut(s) 621, 667, 1312, 1647, 2099
BseRI GAGGAG 1 cut(s) 36
BseSI GKGCMC 2 cut(s) 1630, 2221
BseXI GCAGC 6 cut(s) 299, 302, 327, 1468, 1690, 2002
BshFI GGCC 2 cut(s) 204, 1276
BshNI GGYRCC 1 cut(s) 509
BsiHKAI GWGCWC 1 cut(s) 386
BsiHKCI CYCGRG 3 cut(s) 194, 1868, 2198
BsiSI CCGG 1 cut(s) 1391
BslFI GGGAC 3 cut(s) 280, 855, 1284
BslI CCNNNNNNNGG 4 cut(s) 109, 334, 519, 1328
BsmAI GTCTC 3 cut(s) 162, 227, 2291
BsmFI GGGAC 3 cut(s) 280, 855, 1284
BsmI GAATGC 1 cut(s) 1090
BsnI GGCC 2 cut(s) 204, 1276
BsoBI CYCGRG 3 cut(s) 194, 1868, 2198
Bsp119I TTCGAA 1 cut(s) 1689
Bsp1286I GDGCHC 3 cut(s) 386, 1630, 2221
Bsp13I TCCGGA 1 cut(s) 1390
Bsp1407I TGTACA 1 cut(s) 759
Bsp143I GATC 5 cut(s) 729, 1134, 1384, 2137, 2224
Bsp1720I GCTNAGC 2 cut(s) 962, 967
Bsp19I CCATGG 3 cut(s) 564, 775, 1894
BspACI CCGC 7 cut(s) 294, 494, 716, 739, 1268, 1602, 1974
BspANI GGCC 2 cut(s) 204, 1276
BspCNI CTCAG 5 cut(s) 70, 223, 289, 541, 975
BspEI TCCGGA 1 cut(s) 1390
BspHI TCATGA 2 cut(s) 1093, 1761
BspLI GGNNCC 5 cut(s) 511, 871, 1277, 1386, 2163
BspMAI CTGCAG 1 cut(s) 1680
BspPI GGATC 3 cut(s) 737, 1379, 1392
BspQI GCTCTTC 3 cut(s) 377, 695, 1114
BspT104I TTCGAA 1 cut(s) 1689
BspT107I GGYRCC 1 cut(s) 509
BspTI CTTAAG 1 cut(s) 503
BsrDI GCAATG 2 cut(s) 212, 1406
BsrGI TGTACA 1 cut(s) 759
BsrI ACTGG 5 cut(s) 621, 667, 1312, 1647, 2099
BssMI GATC 5 cut(s) 729, 1134, 1384, 2137, 2224
BssNAI GTATAC 1 cut(s) 1508
BssSI CACGAG 1 cut(s) 45
BssT1I CCWWGG 6 cut(s) 107, 513, 564, 775, 1322, 1894
Bst1107I GTATAC 1 cut(s) 1508
Bst2BI CACGAG 1 cut(s) 45
Bst2UI CCWGG 4 cut(s) 884, 1235, 1280, 1883
Bst4CI ACNGT 2 cut(s) 1550, 1741
Bst6I CTCTTC 4 cut(s) 377, 695, 1114, 1613
BstAFI CTTAAG 1 cut(s) 503
BstAUI TGTACA 1 cut(s) 759
BstBI TTCGAA 1 cut(s) 1689
BstC8I GCNNGC 4 cut(s) 348, 1419, 1757, 2127
BstDEI CTNAG 8 cut(s) 78, 231, 276, 528, 962, 967, 2180, 2274
BstDSI CCRYGG 4 cut(s) 564, 775, 1113, 1894
BstEII GGTNACC 1 cut(s) 665
BstENI CCTNNNNNAGG 1 cut(s) 517
BstF5I GGATG 4 cut(s) 103, 134, 625, 1288
BstKTI GATC 5 cut(s) 732, 1137, 1387, 2140, 2227
BstMAI GTCTC 3 cut(s) 162, 227, 2291
BstMBI GATC 5 cut(s) 729, 1134, 1384, 2137, 2224
BstMWI GCNNNNNNNGC 2 cut(s) 293, 1634
BstNI CCWGG 4 cut(s) 884, 1235, 1280, 1883
BstNSI RCATGY 1 cut(s) 370
BstPI GGTNACC 1 cut(s) 665
BstSCI CCNGG 4 cut(s) 882, 1233, 1278, 1881
BstSFI CTRYAG 4 cut(s) 178, 585, 928, 1676
BstSLI GKGCMC 2 cut(s) 1630, 2221
BstV1I GCAGC 6 cut(s) 299, 302, 327, 1468, 1690, 2002
BstX2I RGATCY 2 cut(s) 1384, 2137
BstXI CCANNNNNNTGG 3 cut(s) 1215, 1241, 2237
BstYI RGATCY 2 cut(s) 1384, 2137
BstZ17I GTATAC 1 cut(s) 1508
BsuRI GGCC 2 cut(s) 204, 1276
BtgI CCRYGG 4 cut(s) 564, 775, 1113, 1894
BtrI CACGTC 1 cut(s) 1079
BtsCI GGATG 4 cut(s) 103, 134, 625, 1288
BtsI GCAGTG 2 cut(s) 207, 839
BtsIMutI CAGTG 4 cut(s) 207, 839, 1319, 1705
Cac8I GCNNGC 4 cut(s) 348, 1419, 1757, 2127
CciI TCATGA 2 cut(s) 1093, 1761
Cfr13I GGNCC 3 cut(s) 869, 902, 1275
CseI GACGC 1 cut(s) 292
Csp6I GTAC 5 cut(s) 760, 824, 1537, 1551, 1958
CviQI GTAC 5 cut(s) 760, 824, 1537, 1551, 1958
DdeI CTNAG 8 cut(s) 78, 231, 276, 528, 962, 967, 2180, 2274
DpnI GATC 5 cut(s) 731, 1136, 1386, 2139, 2226
DpnII GATC 5 cut(s) 729, 1134, 1384, 2137, 2224
Eam1104I CTCTTC 4 cut(s) 377, 695, 1114, 1613
EarI CTCTTC 4 cut(s) 377, 695, 1114, 1613
EciI GGCGGA 2 cut(s) 509, 1617
Eco130I CCWWGG 6 cut(s) 107, 513, 564, 775, 1322, 1894
Eco32I GATATC 1 cut(s) 145
Eco47I GGWCC 2 cut(s) 869, 902
Eco57I CTGAAG 2 cut(s) 135, 1278
Eco88I CYCGRG 3 cut(s) 194, 1868, 2198
Eco91I GGTNACC 1 cut(s) 665
EcoNI CCTNNNNNAGG 1 cut(s) 517
EcoO109I RGGNCCY 2 cut(s) 869, 902
EcoO65I GGTNACC 1 cut(s) 665
EcoRII CCWGG 4 cut(s) 882, 1233, 1278, 1881
EcoRV GATATC 1 cut(s) 145
EcoT14I CCWWGG 6 cut(s) 107, 513, 564, 775, 1322, 1894
EcoT22I ATGCAT 2 cut(s) 451, 655
ErhI CCWWGG 6 cut(s) 107, 513, 564, 775, 1322, 1894
FalI AAGNNNNNCTT 5 cut(s) 497, 529, 689, 721, 2284
FaqI GGGAC 3 cut(s) 280, 855, 1284
FauI CCCGC 2 cut(s) 732, 1261
FauNDI CATATG 1 cut(s) 957
FbaI TGATCA 1 cut(s) 1134
FblI GTMKAC 1 cut(s) 1507
Fnu4HI GCNGC 7 cut(s) 288, 291, 294, 316, 1482, 1679, 2016
FokI GGATG 4 cut(s) 90, 121, 632, 1295
Fsp4HI GCNGC 7 cut(s) 288, 291, 294, 316, 1482, 1679, 2016
FspBI CTAG 4 cut(s) 242, 794, 1062, 1682
GluI GCNGC 7 cut(s) 288, 291, 294, 316, 1482, 1679, 2016
GsuI CTGGAG 3 cut(s) 144, 905, 1217
HaeIII GGCC 2 cut(s) 204, 1276
HapII CCGG 1 cut(s) 1391
HgaI GACGC 1 cut(s) 292
HincII GTYRAC 1 cut(s) 2115
HindII GTYRAC 1 cut(s) 2115
HindIII AAGCTT 1 cut(s) 1200
HinfI GANTC 7 cut(s) 174, 560, 590, 681, 812, 1708, 2111
HpaII CCGG 1 cut(s) 1391
HphI GGTGA 3 cut(s) 659, 1197, 2219
Hpy166II GTNNAC 3 cut(s) 880, 1508, 2115
Hpy8I GTNNAC 3 cut(s) 880, 1508, 2115
Hpy99I CGWCG 2 cut(s) 143, 308
HpyAV CCTTC 6 cut(s) 46, 893, 1449, 1660, 1685, 2015
HpyCH4III ACNGT 2 cut(s) 1550, 1741
HpyCH4IV ACGT 3 cut(s) 246, 1078, 1195
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 1634
HpyF3I CTNAG 8 cut(s) 78, 231, 276, 528, 962, 967, 2180, 2274
HpySE526I ACGT 3 cut(s) 246, 1078, 1195
Kpn2I TCCGGA 1 cut(s) 1390
Ksp22I TGATCA 1 cut(s) 1134
Kzo9I GATC 5 cut(s) 729, 1134, 1384, 2137, 2224
LguI GCTCTTC 3 cut(s) 377, 695, 1114
LmnI GCTCC 4 cut(s) 136, 381, 1548, 2191
Lsp1109I GCAGC 6 cut(s) 299, 302, 327, 1468, 1690, 2002
LweI GCATC 4 cut(s) 662, 1390, 1420, 2023
MaeI CTAG 4 cut(s) 242, 794, 1062, 1682
MaeII ACGT 3 cut(s) 246, 1078, 1195
MaeIII GTNAC 5 cut(s) 416, 576, 665, 1072, 1332
MalI GATC 5 cut(s) 731, 1136, 1386, 2139, 2226
MboI GATC 5 cut(s) 729, 1134, 1384, 2137, 2224
MfeI CAATTG 1 cut(s) 755
MflI RGATCY 2 cut(s) 1384, 2137
MhlI GDGCHC 3 cut(s) 386, 1630, 2221
MlyI GAGTC 2 cut(s) 168, 2120
MmeI TCCRAC 4 cut(s) 162, 286, 915, 2034
Mph1103I ATGCAT 2 cut(s) 451, 655
MroI TCCGGA 1 cut(s) 1390
MroXI GAANNNNTTC 3 cut(s) 951, 1020, 1430
MslI CAYNNNNRTG 3 cut(s) 982, 1213, 2235
MspCI CTTAAG 1 cut(s) 503
MspI CCGG 1 cut(s) 1391
MspR9I CCNGG 4 cut(s) 884, 1235, 1280, 1883
MunI CAATTG 1 cut(s) 755
Mva1269I GAATGC 1 cut(s) 1090
MvaI CCWGG 4 cut(s) 884, 1235, 1280, 1883
MwoI GCNNNNNNNGC 2 cut(s) 293, 1634
NcoI CCATGG 3 cut(s) 564, 775, 1894
NdeI CATATG 1 cut(s) 957
NdeII GATC 5 cut(s) 729, 1134, 1384, 2137, 2224
NlaIV GGNNCC 5 cut(s) 511, 871, 1277, 1386, 2163
NmuCI GTSAC 2 cut(s) 665, 1332
NsiI ATGCAT 2 cut(s) 451, 655
NspI RCATGY 1 cut(s) 370
NspV TTCGAA 1 cut(s) 1689
OliI CACNNNNGTG 1 cut(s) 2235
PaeR7I CTCGAG 1 cut(s) 194
PagI TCATGA 2 cut(s) 1093, 1761
PasI CCCWGGG 1 cut(s) 1882
PciSI GCTCTTC 3 cut(s) 377, 695, 1114
PcsI WCGNNNNNNNCGW 1 cut(s) 1110
PctI GAATGC 1 cut(s) 1090
PdmI GAANNNNTTC 3 cut(s) 951, 1020, 1430
PfeI GAWTC 5 cut(s) 560, 590, 681, 812, 1708
PkrI GCNGC 7 cut(s) 289, 292, 295, 317, 1483, 1680, 2017
PleI GAGTC 2 cut(s) 168, 2119
PpsI GAGTC 2 cut(s) 168, 2119
PpuMI RGGWCCY 2 cut(s) 869, 902
PshBI ATTAAT 2 cut(s) 185, 785
Psp5II RGGWCCY 2 cut(s) 869, 902
Psp6I CCWGG 4 cut(s) 882, 1233, 1278, 1881
PspEI GGTNACC 1 cut(s) 665
PspGI CCWGG 4 cut(s) 882, 1233, 1278, 1881
PspN4I GGNNCC 5 cut(s) 511, 871, 1277, 1386, 2163
PspPI GGNCC 3 cut(s) 869, 902, 1275
PspPPI RGGWCCY 2 cut(s) 869, 902
PstI CTGCAG 1 cut(s) 1680
PsuI RGATCY 2 cut(s) 1384, 2137
RsaI GTAC 5 cut(s) 761, 825, 1538, 1552, 1959
RsaNI GTAC 5 cut(s) 760, 824, 1537, 1551, 1958
RseI CAYNNNNRTG 3 cut(s) 982, 1213, 2235
SapI GCTCTTC 3 cut(s) 377, 695, 1114
SatI GCNGC 7 cut(s) 288, 291, 294, 316, 1482, 1679, 2016
Sau3AI GATC 5 cut(s) 729, 1134, 1384, 2137, 2224
Sau96I GGNCC 3 cut(s) 869, 902, 1275
SchI GAGTC 2 cut(s) 168, 2120
ScrFI CCNGG 4 cut(s) 884, 1235, 1280, 1883
SduI GDGCHC 3 cut(s) 386, 1630, 2221
SfaNI GCATC 4 cut(s) 662, 1390, 1420, 2023
SfcI CTRYAG 4 cut(s) 178, 585, 928, 1676
Sfr274I CTCGAG 1 cut(s) 194
SfuI TTCGAA 1 cut(s) 1689
SinI GGWCC 2 cut(s) 869, 902
SlaI CTCGAG 1 cut(s) 194
SmiMI CAYNNNNRTG 3 cut(s) 982, 1213, 2235
SmlI CTYRAG 4 cut(s) 194, 503, 827, 1055
SmoI CTYRAG 4 cut(s) 194, 503, 827, 1055
SsiI CCGC 7 cut(s) 294, 494, 716, 739, 1268, 1602, 1974
SspI AATATT 2 cut(s) 859, 1019
SspMI CTAG 4 cut(s) 242, 794, 1062, 1682
StyD4I CCNGG 4 cut(s) 882, 1233, 1278, 1881
StyI CCWWGG 6 cut(s) 107, 513, 564, 775, 1322, 1894
TaaI ACNGT 2 cut(s) 1550, 1741
TaiI ACGT 3 cut(s) 249, 1081, 1198
TaqI TCGA 6 cut(s) 172, 195, 442, 1564, 1664, 1689
TatI WGTACW 2 cut(s) 759, 823
TauI GCSGC 1 cut(s) 296
TfiI GAWTC 5 cut(s) 560, 590, 681, 812, 1708
TscAI CASTG 4 cut(s) 214, 839, 1319, 1705
TseFI GTSAC 2 cut(s) 665, 1332
TseI GCWGC 6 cut(s) 287, 290, 315, 1481, 1678, 2015
Tsp45I GTSAC 2 cut(s) 665, 1332
TspGWI ACGGA 2 cut(s) 595, 1102
TspRI CASTG 4 cut(s) 214, 839, 1319, 1705
Vha464I CTTAAG 1 cut(s) 503
VpaK11BI GGWCC 2 cut(s) 869, 902
VspI ATTAAT 2 cut(s) 185, 785
XagI CCTNNNNNAGG 1 cut(s) 517
XapI RAATTY 4 cut(s) 458, 1039, 1746, 1795
XceI RCATGY 1 cut(s) 370
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XhoI CTCGAG 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 1507
XmnI GAANNNNTTC 3 cut(s) 951, 1020, 1430
XspI CTAG 4 cut(s) 242, 794, 1062, 1682
Zsp2I ATGCAT 2 cut(s) 451, 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.