RchiOBHm_Chr5g0072951

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000183
Physical Location & Seq
Reverse (-)
79000183 .. 79005748
5566 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34787

Sequence Viewer

Length: 2037 bp
ATGGCCTCACTGCATTGCTCATTCATGCCTGAGATTTACCCACAAGGGCCCAAAAGCCTTGAACCCAGTGAAGAATATGAAGCAGCCAAAGGTGGAGATGGTGCCTCTGAGACTAAGACGGCAGCAGCAGCAATGTGCGTTGGAATAGGCAGCATGTCTGACCCTGTTGAGGCACAAGGGCTTGCACACTTCCTAGAACACATGCTCTTTATGGGGAGTACAAAGTTTCCTATTGAAAATGAGTTCGGTAGTTACTTGTCCAAGAATGGAGGATGGTCAAATGCATACACAGAAGCAGAGCAGACTTGCTACTATTTTGTTGTAAAACGAGAGTTTCTAAAGGGTGCCTTATCAAGATTTTCTCAGTTCTTTGTTTCACCTCTAATGAAAAGAGAAGCCATGGAGCGAGAGTTACAGGCTATAGATTTAGAGTTTAACAGGGTTCTGCAAAACGATTTTTGCCTCCTTCAGCAACTTCAATGCTATACATCCTCACCTGGTCACCCATTTAACAAATTCTCTTGGGGAAATAAGAAGAGCTTGGTTAATGCAAAAGAAAAAGGGATCGACTTGCGTGAACAAATAATGAAATTTTACAGTGACTATTACCATGGTGGATTAATGAAGCTAGTTGTCATTGGTGGAGAATCTCTTGATGTACTTGAGGAGTGGATTTTAGAACTGTATGGAGATGTTAAAAGTGGTCCCCAAGTAAATCTGGAGTTCAAGGCAGAAGGTCCTATATGGAAATCTGGAAAACTTTACAGGCTAGAGGCTGTTGACAATGTTCATATACTCCACCTAGCATGGACACTTCCATGTCTTCATCGACACTATTTGAAAAGCCCAACATATTATTTACGTCATCTCCTTGAGCATGAGGGCAGGGGAAGTTTGTATTTCCACCTCAAAGCTAGAGGTTGGGCAACATTTGTAAGTGCTCCAGTGGAACACTATTCTGTGGCTGATGTCTTCTGCATGATCATATACCTCACTGACTCTGGATTGGAGAAGATTTTCGATATAATTGGGTTGGTGTATCAATACATTAAGTTCTTACGGCAAGTGTTGCCCCAAGAATGGATATTTCGTGAACTCCAGGATATTGGGAACATCTACCTTAAGTTTTTAGAGGAGCAGCCCCAGGATTATTATGCTTTAAGACTTGGAGGAAATTTACTACATTATCCCGCAGAGCATGCTATTTATGGGGACTATTTGCTAGAGAATTGGGATGAGAAATTGATAGAATATACTCTAGGTTTCTTGAGACCAGAGAACATGAGGATTGATGTGATATCTAAGTCCTCCACGTTGTCAGAAGATTTCCAGTGCGACCCTTGGTTTGGGTCACATTTTACCGAGGAAGATATATTTCCGTCTTTGATAGATTTATGGAAGGATCCTCAAGAAATTGATAGTTCATTGCATCTCCCAGAAAAGAATGAATTCATTCCCTCTGATTTTTCCATCTGTTCTGATGTTCTTGATACCGCAAATACATCTTGTCCTAGATGTATACTTGATGGGCCATTGGTGAAGTTATGGTACAAGCTTGATAGTACTTACAAACTTCCGCAGGCAAACATATACTTTCGTATCAGTCTAAAGGAAGCATCTGGTAGTGTGAAGAGTTCTGTCTTGACTCAACTATATGGCGACCTTCTTTGGGATGAGCTGAATGAGGTTGTATATCAGGCTAGTGTCGCCCAGCTGGGAACTTCTGTATCTGTGTACACTAACTATCTGGAGCTGAAGGTCAGCGGTTTCAATGATAAGATTCCAACTCTCTTGTCAAAAATTATGACAACAGCCAAAAATTTCTCGCCAACTTACGAATGCTTCAAGGTTATTAAAGAGGACATGGAGCGAGCATATAAGAACACCAATATGGATCCTTGGAGCCACTCGACATACTTGAGAGATGAAGTTTTGTACCAAAATTTCTGTGATGTAGATGAGACGTTGCATGTTTTAAATGGATTGACTGTTTCTGATGTGAAGTCATTCATTCCCGAGCTTTGGTCCCCGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

678

Amino Acids

77.84

Weight (kDa)

5.06

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 37 - 152 1.3e-30 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 192 - 367 2.9e-18 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 375 - 657 6.7e-80 Middle or third domain of peptidase_M16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 101, 344
AccI GTMKAC 1 cut(s) 1519
AciI CCGC 4 cut(s) 1191, 1494, 1577, 1764
AclWI GGATC 5 cut(s) 572, 1397, 1410, 1889, 1902
AcsI RAATTY 6 cut(s) 515, 590, 1174, 1448, 1819, 1942
AcuI CTGAAG 2 cut(s) 452, 1775
AfaI GTAC 6 cut(s) 220, 660, 1550, 1564, 1736, 1937
AfiI CCNNNNNNNGG 5 cut(s) 169, 1080, 1346, 1669, 2022
AflII CTTAAG 1 cut(s) 1121
AgsI TTSAA 7 cut(s) 62, 236, 479, 727, 841, 1771, 1846
AjnI CCWGG 3 cut(s) 496, 1098, 1143
AluBI AGCT 8 cut(s) 540, 628, 914, 1555, 1678, 1714, 1753, 2020
AluI AGCT 8 cut(s) 540, 628, 914, 1555, 1678, 1714, 1753, 2020
Alw21I GWGCWC 1 cut(s) 943
Alw26I GTCTC 3 cut(s) 104, 1264, 1955
AlwI GGATC 5 cut(s) 572, 1397, 1410, 1889, 1902
Ama87I CYCGRG 1 cut(s) 2015
AoxI GGCC 3 cut(s) 3, 47, 1529
ApaI GGGCCC 1 cut(s) 51
ApeKI GCWGC 6 cut(s) 83, 122, 125, 128, 150, 1138
ApoI RAATTY 6 cut(s) 515, 590, 1174, 1448, 1819, 1942
ArsI GACNNNNNNTTYG 2 cut(s) 1328, 1360
AseI ATTAAT 1 cut(s) 620
Asp700I GAANNNNTTC 4 cut(s) 1016, 1448, 1452, 2006
AspS9I GGNCC 6 cut(s) 47, 48, 704, 737, 1529, 2025
AsuC2I CCSGG 1 cut(s) 2030
AsuHPI GGTGA 4 cut(s) 369, 486, 494, 1549
AvaI CYCGRG 1 cut(s) 2015
AvaII GGWCC 3 cut(s) 704, 737, 2025
BaeGI GKGCMC 1 cut(s) 51
BaeI ACNNNNGTAYC 4 cut(s) 1582, 1615, 1710, 1743
BamHI GGATCC 2 cut(s) 1402, 1894
BanI GGYRCC 2 cut(s) 101, 344
BanII GRGCYC 1 cut(s) 51
BarI GAAGNNNNNNTAC 2 cut(s) 1532, 1564
BbsI GAAGAC 2 cut(s) 815, 964
Bbv12I GWGCWC 1 cut(s) 943
BbvI GCAGC 6 cut(s) 95, 134, 137, 140, 162, 1150
BccI CCATC 4 cut(s) 92, 267, 1478, 1520
BceAI ACGGC 2 cut(s) 135, 1076
BciT130I CCWGG 3 cut(s) 498, 1100, 1145
BclI TGATCA 1 cut(s) 981
BcnI CCSGG 1 cut(s) 2030
BcoDI GTCTC 3 cut(s) 104, 1264, 1955
BfaI CTAG 9 cut(s) 194, 629, 770, 803, 915, 1223, 1259, 1512, 1701
BfmI CTRYAG 1 cut(s) 420
BfrI CTTAAG 1 cut(s) 1121
BisI GCNGC 6 cut(s) 84, 123, 126, 129, 151, 1139
BlsI GCNGC 6 cut(s) 85, 124, 127, 130, 152, 1140
BmcAI AGTACT 1 cut(s) 1564
Bme1390I CCNGG 4 cut(s) 498, 1100, 1145, 2030
Bme18I GGWCC 3 cut(s) 704, 737, 2025
BmeT110I CYCGRG 1 cut(s) 2015
BmgT120I GGNCC 6 cut(s) 47, 48, 704, 737, 1529, 2025
BmiI GGNNCC 8 cut(s) 49, 103, 346, 706, 1404, 1896, 1904, 2027
BmrFI CCNGG 4 cut(s) 498, 1100, 1145, 2030
BmrI ACTGGG 1 cut(s) 60
BmsI GCATC 2 cut(s) 1438, 1625
BmuI ACTGGG 1 cut(s) 60
BpiI GAAGAC 2 cut(s) 815, 964
BpmI CTGGAG 4 cut(s) 740, 927, 1082, 1769
BpuEI CTTGAG 5 cut(s) 683, 893, 1288, 1392, 1939
BpuMI CCSGG 1 cut(s) 2030
BsaI GGTCTC 1 cut(s) 1264
BsaJI CCNNGG 7 cut(s) 399, 610, 1143, 1340, 1362, 1898, 2028
BsaXI ACNNNNNCTCC 4 cut(s) 395, 425, 852, 882
Bsc4I CCNNNNNNNGG 5 cut(s) 169, 1080, 1346, 1669, 2022
Bse1I ACTGG 3 cut(s) 66, 944, 1330
Bse3DI GCAATG 3 cut(s) 13, 138, 1424
BseBI CCWGG 3 cut(s) 498, 1100, 1145
BseDI CCNNGG 7 cut(s) 399, 610, 1143, 1340, 1362, 1898, 2028
BseGI GGATG 4 cut(s) 278, 488, 1240, 1678
BseLI CCNNNNNNNGG 5 cut(s) 169, 1080, 1346, 1669, 2022
BseMI GCAATG 3 cut(s) 13, 138, 1424
BseMII CTCAG 3 cut(s) 21, 99, 377
BseNI ACTGG 3 cut(s) 66, 944, 1330
BseRI GAGGAG 2 cut(s) 680, 1148
BseSI GKGCMC 1 cut(s) 51
BseXI GCAGC 6 cut(s) 95, 134, 137, 140, 162, 1150
BseYI CCCAGC 2 cut(s) 1710, 1714
BshFI GGCC 3 cut(s) 5, 49, 1531
BshNI GGYRCC 2 cut(s) 101, 344
BsiHKAI GWGCWC 1 cut(s) 943
BsiHKCI CYCGRG 1 cut(s) 2015
BsiSI CCGG 1 cut(s) 2030
BslFI GGGAC 3 cut(s) 690, 1226, 2011
BslI CCNNNNNNNGG 5 cut(s) 169, 1080, 1346, 1669, 2022
BsmAI GTCTC 3 cut(s) 104, 1264, 1955
BsmBI CGTCTC 1 cut(s) 1955
BsmFI GGGAC 3 cut(s) 690, 1226, 2011
BsmI GAATGC 1 cut(s) 1844
BsnI GGCC 3 cut(s) 5, 49, 1531
Bso31I GGTCTC 1 cut(s) 1264
BsoBI CYCGRG 1 cut(s) 2015
Bsp120I GGGCCC 1 cut(s) 47
Bsp1286I GDGCHC 2 cut(s) 51, 943
Bsp1407I TGTACA 1 cut(s) 1734
Bsp143I GATC 4 cut(s) 564, 981, 1402, 1894
Bsp19I CCATGG 2 cut(s) 399, 610
BspACI CCGC 4 cut(s) 1191, 1494, 1577, 1764
BspANI GGCC 3 cut(s) 5, 49, 1531
BspCNI CTCAG 3 cut(s) 22, 100, 376
BspLI GGNNCC 8 cut(s) 49, 103, 346, 706, 1404, 1896, 1904, 2027
BspPI GGATC 5 cut(s) 572, 1397, 1410, 1889, 1902
BspQI GCTCTTC 1 cut(s) 530
BspT107I GGYRCC 2 cut(s) 101, 344
BspTI CTTAAG 1 cut(s) 1121
BspTNI GGTCTC 1 cut(s) 1264
BsrDI GCAATG 3 cut(s) 13, 138, 1424
BsrGI TGTACA 1 cut(s) 1734
BsrI ACTGG 3 cut(s) 66, 944, 1330
BssECI CCNNGG 7 cut(s) 399, 610, 1143, 1340, 1362, 1898, 2028
BssMI GATC 4 cut(s) 564, 981, 1402, 1894
BssNAI GTATAC 1 cut(s) 1520
BssT1I CCWWGG 4 cut(s) 399, 610, 1340, 1898
Bst1107I GTATAC 1 cut(s) 1520
Bst2UI CCWGG 3 cut(s) 498, 1100, 1145
Bst4CI ACNGT 3 cut(s) 599, 684, 1990
Bst6I CTCTTC 2 cut(s) 530, 1625
BstAFI CTTAAG 1 cut(s) 1121
BstAPI GCANNNNNTGC 2 cut(s) 1069, 1199
BstAUI TGTACA 1 cut(s) 1734
BstC8I GCNNGC 4 cut(s) 183, 1200, 1581, 1872
BstDEI CTNAG 5 cut(s) 30, 108, 114, 363, 1302
BstDSI CCRYGG 2 cut(s) 399, 610
BstEII GGTNACC 1 cut(s) 500
BstF5I GGATG 4 cut(s) 278, 488, 1240, 1678
BstKTI GATC 4 cut(s) 567, 984, 1405, 1897
BstMAI GTCTC 3 cut(s) 104, 1264, 1955
BstMBI GATC 4 cut(s) 564, 981, 1402, 1894
BstMWI GCNNNNNNNGC 4 cut(s) 128, 1069, 1199, 1706
BstNI CCWGG 3 cut(s) 498, 1100, 1145
BstNSI RCATGY 4 cut(s) 157, 205, 1202, 1973
BstPI GGTNACC 1 cut(s) 500
BstSCI CCNGG 4 cut(s) 496, 1098, 1143, 2028
BstSFI CTRYAG 1 cut(s) 420
BstSLI GKGCMC 1 cut(s) 51
BstV1I GCAGC 6 cut(s) 95, 134, 137, 140, 162, 1150
BstV2I GAAGAC 2 cut(s) 815, 964
BstX2I RGATCY 2 cut(s) 1402, 1894
BstXI CCANNNNNNTGG 1 cut(s) 1106
BstYI RGATCY 2 cut(s) 1402, 1894
BstZ17I GTATAC 1 cut(s) 1520
BsuRI GGCC 3 cut(s) 5, 49, 1531
BtgI CCRYGG 2 cut(s) 399, 610
BtsCI GGATG 4 cut(s) 278, 488, 1240, 1678
BtsI GCAGTG 1 cut(s) 8
BtsIMutI CAGTG 6 cut(s) 8, 73, 604, 951, 993, 1337
Cac8I GCNNGC 4 cut(s) 183, 1200, 1581, 1872
Cfr13I GGNCC 6 cut(s) 47, 48, 704, 737, 1529, 2025
CsiI ACCWGGT 1 cut(s) 496
Csp6I GTAC 6 cut(s) 219, 659, 1549, 1563, 1735, 1936
CviQI GTAC 6 cut(s) 219, 659, 1549, 1563, 1735, 1936
DdeI CTNAG 5 cut(s) 30, 108, 114, 363, 1302
DpnI GATC 4 cut(s) 566, 983, 1404, 1896
DpnII GATC 4 cut(s) 564, 981, 1402, 1894
DraI TTTAAA 1 cut(s) 1977
Eam1104I CTCTTC 2 cut(s) 530, 1625
EarI CTCTTC 2 cut(s) 530, 1625
Eco130I CCWWGG 4 cut(s) 399, 610, 1340, 1898
Eco24I GRGCYC 1 cut(s) 51
Eco31I GGTCTC 1 cut(s) 1264
Eco32I GATATC 1 cut(s) 1299
Eco47I GGWCC 3 cut(s) 704, 737, 2025
Eco57I CTGAAG 2 cut(s) 452, 1775
Eco88I CYCGRG 1 cut(s) 2015
Eco91I GGTNACC 1 cut(s) 500
EcoO109I RGGNCCY 2 cut(s) 47, 737
EcoO65I GGTNACC 1 cut(s) 500
EcoRI GAATTC 1 cut(s) 1448
EcoRII CCWGG 3 cut(s) 496, 1098, 1143
EcoRV GATATC 1 cut(s) 1299
EcoT14I CCWWGG 4 cut(s) 399, 610, 1340, 1898
EcoT22I ATGCAT 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 51
ErhI CCWWGG 4 cut(s) 399, 610, 1340, 1898
Esp3I CGTCTC 1 cut(s) 1955
FalI AAGNNNNNCTT 4 cut(s) 332, 364, 524, 556
FaqI GGGAC 3 cut(s) 690, 1226, 2011
FauI CCCGC 1 cut(s) 1198
FbaI TGATCA 1 cut(s) 981
FblI GTMKAC 1 cut(s) 1519
Fnu4HI GCNGC 6 cut(s) 84, 123, 126, 129, 151, 1139
FokI GGATG 4 cut(s) 285, 475, 1247, 1685
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 6 cut(s) 84, 123, 126, 129, 151, 1139
FspBI CTAG 9 cut(s) 194, 629, 770, 803, 915, 1223, 1259, 1512, 1701
GluI GCNGC 6 cut(s) 84, 123, 126, 129, 151, 1139
GsaI CCCAGC 2 cut(s) 1714, 1718
GsuI CTGGAG 4 cut(s) 740, 927, 1082, 1769
HaeIII GGCC 3 cut(s) 5, 49, 1531
HapII CCGG 1 cut(s) 2030
HincII GTYRAC 1 cut(s) 781
HindII GTYRAC 1 cut(s) 781
HindIII AAGCTT 1 cut(s) 1553
HinfI GANTC 4 cut(s) 647, 998, 1645, 1780
HpaII CCGG 1 cut(s) 2030
HphI GGTGA 4 cut(s) 369, 486, 494, 1549
Hpy166II GTNNAC 6 cut(s) 578, 781, 1094, 1520, 1735, 1737
Hpy188I TCNGA 6 cut(s) 109, 160, 1321, 1462, 1480, 1996
Hpy8I GTNNAC 6 cut(s) 578, 781, 1094, 1520, 1735, 1737
HpyAV CCTTC 5 cut(s) 476, 728, 1393, 1673, 1750
HpyCH4III ACNGT 3 cut(s) 599, 684, 1990
HpyCH4IV ACGT 3 cut(s) 862, 1313, 1964
HpyCH4V TGCA 8 cut(s) 13, 185, 284, 448, 551, 978, 1429, 1969
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 1069, 1199, 1706
HpyF3I CTNAG 5 cut(s) 30, 108, 114, 363, 1302
HpySE526I ACGT 3 cut(s) 862, 1313, 1964
Ksp22I TGATCA 1 cut(s) 981
Kzo9I GATC 4 cut(s) 564, 981, 1402, 1894
LguI GCTCTTC 1 cut(s) 530
LmnI GCTCC 6 cut(s) 403, 946, 1135, 1750, 1867, 1902
Lsp1109I GCAGC 6 cut(s) 95, 134, 137, 140, 162, 1150
LweI GCATC 2 cut(s) 1438, 1625
MabI ACCWGGT 1 cut(s) 496
MaeI CTAG 9 cut(s) 194, 629, 770, 803, 915, 1223, 1259, 1512, 1701
MaeII ACGT 3 cut(s) 862, 1313, 1964
MaeIII GTNAC 5 cut(s) 251, 411, 500, 599, 1350
MalI GATC 4 cut(s) 566, 983, 1404, 1896
MboI GATC 4 cut(s) 564, 981, 1402, 1894
MboII GAAGA 8 cut(s) 83, 547, 815, 964, 1024, 1334, 1379, 1642
MflI RGATCY 2 cut(s) 1402, 1894
MhlI GDGCHC 2 cut(s) 51, 943
MlyI GAGTC 2 cut(s) 992, 1639
MmeI TCCRAC 2 cut(s) 121, 1808
Mph1103I ATGCAT 1 cut(s) 286
MroXI GAANNNNTTC 4 cut(s) 1016, 1448, 1452, 2006
MslI CAYNNNNRTG 2 cut(s) 817, 1889
MspA1I CMGCKG 2 cut(s) 1714, 1764
MspCI CTTAAG 1 cut(s) 1121
MspI CCGG 1 cut(s) 2030
MspR9I CCNGG 4 cut(s) 498, 1100, 1145, 2030
Mva1269I GAATGC 1 cut(s) 1844
MvaI CCWGG 3 cut(s) 498, 1100, 1145
MwoI GCNNNNNNNGC 4 cut(s) 128, 1069, 1199, 1706
NciI CCSGG 1 cut(s) 2030
NcoI CCATGG 2 cut(s) 399, 610
NdeII GATC 4 cut(s) 564, 981, 1402, 1894
NlaIV GGNNCC 8 cut(s) 49, 103, 346, 706, 1404, 1896, 1904, 2027
NmuCI GTSAC 3 cut(s) 500, 599, 1350
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 4 cut(s) 157, 205, 1202, 1973
PaeI GCATGC 1 cut(s) 1202
PciSI GCTCTTC 1 cut(s) 530
PctI GAATGC 1 cut(s) 1844
PdmI GAANNNNTTC 4 cut(s) 1016, 1448, 1452, 2006
PfeI GAWTC 2 cut(s) 647, 1780
PfoI TCCNGGA 1 cut(s) 1098
PkrI GCNGC 6 cut(s) 85, 124, 127, 130, 152, 1140
PleI GAGTC 2 cut(s) 992, 1639
PpsI GAGTC 2 cut(s) 992, 1639
PpuMI RGGWCCY 1 cut(s) 737
PshBI ATTAAT 1 cut(s) 620
Psp5II RGGWCCY 1 cut(s) 737
Psp6I CCWGG 3 cut(s) 496, 1098, 1143
PspEI GGTNACC 1 cut(s) 500
PspFI CCCAGC 2 cut(s) 1710, 1714
PspGI CCWGG 3 cut(s) 496, 1098, 1143
PspN4I GGNNCC 8 cut(s) 49, 103, 346, 706, 1404, 1896, 1904, 2027
PspOMI GGGCCC 1 cut(s) 47
PspPI GGNCC 6 cut(s) 47, 48, 704, 737, 1529, 2025
PspPPI RGGWCCY 1 cut(s) 737
PsuI RGATCY 2 cut(s) 1402, 1894
PvuII CAGCTG 1 cut(s) 1714
RsaI GTAC 6 cut(s) 220, 660, 1550, 1564, 1736, 1937
RsaNI GTAC 6 cut(s) 219, 659, 1549, 1563, 1735, 1936
RseI CAYNNNNRTG 2 cut(s) 817, 1889
SapI GCTCTTC 1 cut(s) 530
SatI GCNGC 6 cut(s) 84, 123, 126, 129, 151, 1139
Sau3AI GATC 4 cut(s) 564, 981, 1402, 1894
Sau96I GGNCC 6 cut(s) 47, 48, 704, 737, 1529, 2025
ScaI AGTACT 1 cut(s) 1564
SchI GAGTC 2 cut(s) 992, 1639
ScrFI CCNGG 4 cut(s) 498, 1100, 1145, 2030
SduI GDGCHC 2 cut(s) 51, 943
SexAI ACCWGGT 1 cut(s) 496
SfaNI GCATC 2 cut(s) 1438, 1625
SfcI CTRYAG 1 cut(s) 420
SinI GGWCC 3 cut(s) 704, 737, 2025
SmiMI CAYNNNNRTG 2 cut(s) 817, 1889
SmlI CTYRAG 6 cut(s) 662, 872, 1121, 1267, 1407, 1918
SmoI CTYRAG 6 cut(s) 662, 872, 1121, 1267, 1407, 1918
SphI GCATGC 1 cut(s) 1202
SsiI CCGC 4 cut(s) 1191, 1494, 1577, 1764
SspMI CTAG 9 cut(s) 194, 629, 770, 803, 915, 1223, 1259, 1512, 1701
StyD4I CCNGG 4 cut(s) 496, 1098, 1143, 2028
StyI CCWWGG 4 cut(s) 399, 610, 1340, 1898
TaaI ACNGT 3 cut(s) 599, 684, 1990
TaiI ACGT 3 cut(s) 865, 1316, 1967
TaqI TCGA 4 cut(s) 567, 829, 1020, 1910
TatI WGTACW 4 cut(s) 218, 658, 1562, 1734
TfiI GAWTC 2 cut(s) 647, 1780
TscAI CASTG 6 cut(s) 15, 73, 604, 951, 1000, 1337
TseFI GTSAC 3 cut(s) 500, 599, 1350
TseI GCWGC 6 cut(s) 83, 122, 125, 128, 150, 1138
Tsp45I GTSAC 3 cut(s) 500, 599, 1350
TspGWI ACGGA 1 cut(s) 1368
TspRI CASTG 6 cut(s) 15, 73, 604, 951, 1000, 1337
Vha464I CTTAAG 1 cut(s) 1121
VpaK11BI GGWCC 3 cut(s) 704, 737, 2025
VspI ATTAAT 1 cut(s) 620
XapI RAATTY 6 cut(s) 515, 590, 1174, 1448, 1819, 1942
XceI RCATGY 4 cut(s) 157, 205, 1202, 1973
XmiI GTMKAC 1 cut(s) 1519
XmnI GAANNNNTTC 4 cut(s) 1016, 1448, 1452, 2006
XspI CTAG 9 cut(s) 194, 629, 770, 803, 915, 1223, 1259, 1512, 1701
ZrmI AGTACT 1 cut(s) 1564
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.