Belongs to the peptidase M16 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 33 |
| Acc36I | ACCTGC | 1 cut(s) | 838 |
| AccBSI | CCGCTC | 1 cut(s) | 427 |
| AccI | GTMKAC | 2 cut(s) | 667, 712 |
| AccII | CGCG | 1 cut(s) | 832 |
| AciI | CCGC | 3 cut(s) | 170, 427, 586 |
| AcsI | RAATTY | 1 cut(s) | 266 |
| AcuI | CTGAAG | 1 cut(s) | 146 |
| AcyI | GRCGYC | 1 cut(s) | 30 |
| AfaI | GTAC | 4 cut(s) | 262, 385, 566, 746 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 211, 771 |
| AgsI | TTSAA | 9 cut(s) | 40, 362, 401, 419, 508, 554, 814, 857, 866 |
| AjnI | CCWGG | 1 cut(s) | 69 |
| AluBI | AGCT | 6 cut(s) | 195, 318, 409, 487, 703, 821 |
| AluI | AGCT | 6 cut(s) | 195, 318, 409, 487, 703, 821 |
| Alw21I | GWGCWC | 1 cut(s) | 705 |
| Alw26I | GTCTC | 2 cut(s) | 48, 769 |
| Ama87I | CYCGRG | 1 cut(s) | 209 |
| ApoI | RAATTY | 1 cut(s) | 266 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 667, 699, 768, 800 |
| AseI | ATTAAT | 1 cut(s) | 464 |
| AsuHPI | GGTGA | 1 cut(s) | 529 |
| AsuII | TTCGAA | 1 cut(s) | 307 |
| AvaI | CYCGRG | 1 cut(s) | 209 |
| BanII | GRGCYC | 1 cut(s) | 705 |
| BbsI | GAAGAC | 2 cut(s) | 624, 864 |
| Bbv12I | GWGCWC | 1 cut(s) | 705 |
| BccI | CCATC | 1 cut(s) | 97 |
| BciT130I | CCWGG | 1 cut(s) | 71 |
| BcoDI | GTCTC | 2 cut(s) | 48, 769 |
| BfaI | CTAG | 3 cut(s) | 62, 83, 236 |
| BfmI | CTRYAG | 2 cut(s) | 713, 822 |
| BfuAI | ACCTGC | 1 cut(s) | 838 |
| BglII | AGATCT | 1 cut(s) | 469 |
| BlpI | GCTNAGC | 1 cut(s) | 704 |
| Bme1390I | CCNGG | 1 cut(s) | 71 |
| BmeT110I | CYCGRG | 1 cut(s) | 209 |
| BmrFI | CCNGG | 1 cut(s) | 71 |
| BoxI | GACNNNNGTC | 1 cut(s) | 838 |
| BpiI | GAAGAC | 2 cut(s) | 624, 864 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 704 |
| Bpu14I | TTCGAA | 1 cut(s) | 307 |
| BsaHI | GRCGYC | 1 cut(s) | 30 |
| BsaJI | CCNNGG | 1 cut(s) | 210 |
| BsaWI | WCCGGW | 1 cut(s) | 394 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 211, 771 |
| Bse1I | ACTGG | 2 cut(s) | 515, 639 |
| Bse3DI | GCAATG | 2 cut(s) | 180, 303 |
| BseBI | CCWGG | 1 cut(s) | 71 |
| BseDI | CCNNGG | 1 cut(s) | 210 |
| BseGI | GGATG | 4 cut(s) | 100, 112, 643, 727 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 211, 771 |
| BseMI | GCAATG | 2 cut(s) | 180, 303 |
| BseMII | CTCAG | 2 cut(s) | 164, 718 |
| BseNI | ACTGG | 2 cut(s) | 515, 639 |
| BseYI | CCCAGC | 1 cut(s) | 487 |
| BsgI | GTGCAG | 2 cut(s) | 521, 866 |
| Bsh1236I | CGCG | 1 cut(s) | 832 |
| BsiHKAI | GWGCWC | 1 cut(s) | 705 |
| BsiHKCI | CYCGRG | 1 cut(s) | 209 |
| BsiSI | CCGG | 2 cut(s) | 10, 395 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 211, 771 |
| BsmAI | GTCTC | 2 cut(s) | 48, 769 |
| BsmI | GAATGC | 2 cut(s) | 303, 682 |
| BsoBI | CYCGRG | 1 cut(s) | 209 |
| Bsp119I | TTCGAA | 1 cut(s) | 307 |
| Bsp1286I | GDGCHC | 1 cut(s) | 705 |
| Bsp143I | GATC | 1 cut(s) | 469 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 704 |
| Bsp68I | TCGCGA | 1 cut(s) | 832 |
| BspACI | CCGC | 3 cut(s) | 170, 427, 586 |
| BspCNI | CTCAG | 2 cut(s) | 163, 717 |
| BspFNI | CGCG | 1 cut(s) | 832 |
| BspMAI | CTGCAG | 1 cut(s) | 826 |
| BspMI | ACCTGC | 1 cut(s) | 838 |
| BspQI | GCTCTTC | 1 cut(s) | 693 |
| BspT104I | TTCGAA | 1 cut(s) | 307 |
| BsrBI | CCGCTC | 1 cut(s) | 427 |
| BsrDI | GCAATG | 2 cut(s) | 180, 303 |
| BsrI | ACTGG | 2 cut(s) | 515, 639 |
| BssECI | CCNNGG | 1 cut(s) | 210 |
| BssMI | GATC | 1 cut(s) | 469 |
| BssNAI | GTATAC | 1 cut(s) | 668 |
| BssNI | GRCGYC | 1 cut(s) | 30 |
| Bst1107I | GTATAC | 1 cut(s) | 668 |
| Bst2UI | CCWGG | 1 cut(s) | 71 |
| Bst4CI | ACNGT | 1 cut(s) | 902 |
| Bst6I | CTCTTC | 2 cut(s) | 93, 693 |
| BstACI | GRCGYC | 1 cut(s) | 30 |
| BstBI | TTCGAA | 1 cut(s) | 307 |
| BstC8I | GCNNGC | 4 cut(s) | 7, 225, 348, 429 |
| BstDEI | CTNAG | 3 cut(s) | 150, 156, 704 |
| BstF5I | GGATG | 4 cut(s) | 100, 112, 643, 727 |
| BstFNI | CGCG | 1 cut(s) | 832 |
| BstKTI | GATC | 1 cut(s) | 472 |
| BstMAI | GTCTC | 2 cut(s) | 48, 769 |
| BstMBI | GATC | 1 cut(s) | 469 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 170, 293 |
| BstNI | CCWGG | 1 cut(s) | 71 |
| BstNSI | RCATGY | 1 cut(s) | 247 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 838 |
| BstSCI | CCNGG | 1 cut(s) | 69 |
| BstSFI | CTRYAG | 2 cut(s) | 713, 822 |
| BstUI | CGCG | 1 cut(s) | 832 |
| BstV2I | GAAGAC | 2 cut(s) | 624, 864 |
| BstX2I | RGATCY | 1 cut(s) | 469 |
| BstYI | RGATCY | 1 cut(s) | 469 |
| BstZ17I | GTATAC | 1 cut(s) | 668 |
| BtsCI | GGATG | 4 cut(s) | 100, 112, 643, 727 |
| BtsI | GCAGTG | 1 cut(s) | 438 |
| BtsIMutI | CAGTG | 1 cut(s) | 438 |
| BtuMI | TCGCGA | 1 cut(s) | 832 |
| BveI | ACCTGC | 1 cut(s) | 838 |
| Cac8I | GCNNGC | 4 cut(s) | 7, 225, 348, 429 |
| CseI | GACGC | 1 cut(s) | 758 |
| Csp6I | GTAC | 4 cut(s) | 261, 384, 565, 745 |
| CviAII | CATG | 2 cut(s) | 244, 376 |
| CviQI | GTAC | 4 cut(s) | 261, 384, 565, 745 |
| DdeI | CTNAG | 3 cut(s) | 150, 156, 704 |
| DpnI | GATC | 1 cut(s) | 471 |
| DpnII | GATC | 1 cut(s) | 469 |
| Eam1104I | CTCTTC | 2 cut(s) | 93, 693 |
| EarI | CTCTTC | 2 cut(s) | 93, 693 |
| Ecl136II | GAGCTC | 1 cut(s) | 703 |
| Eco24I | GRGCYC | 1 cut(s) | 705 |
| Eco53kI | GAGCTC | 1 cut(s) | 703 |
| Eco57I | CTGAAG | 1 cut(s) | 146 |
| Eco88I | CYCGRG | 1 cut(s) | 209 |
| EcoICRI | GAGCTC | 1 cut(s) | 703 |
| EcoRII | CCWGG | 1 cut(s) | 69 |
| EcoT22I | ATGCAT | 1 cut(s) | 684 |
| EcoT38I | GRGCYC | 1 cut(s) | 705 |
| FaeI | CATG | 2 cut(s) | 247, 379 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 731, 763 |
| FatI | CATG | 2 cut(s) | 243, 375 |
| FauI | CCCGC | 1 cut(s) | 420 |
| FauNDI | CATATG | 1 cut(s) | 366 |
| FblI | GTMKAC | 2 cut(s) | 667, 712 |
| FokI | GGATG | 4 cut(s) | 107, 119, 650, 734 |
| FriOI | GRGCYC | 1 cut(s) | 705 |
| FspBI | CTAG | 3 cut(s) | 62, 83, 236 |
| GsaI | CCCAGC | 1 cut(s) | 491 |
| HapII | CCGG | 2 cut(s) | 10, 395 |
| HgaI | GACGC | 1 cut(s) | 758 |
| Hin1I | GRCGYC | 1 cut(s) | 30 |
| Hin1II | CATG | 2 cut(s) | 247, 379 |
| HincII | GTYRAC | 1 cut(s) | 730 |
| HindII | GTYRAC | 1 cut(s) | 730 |
| HinfI | GANTC | 5 cut(s) | 146, 443, 644, 827, 839 |
| HpaII | CCGG | 2 cut(s) | 10, 395 |
| HphI | GGTGA | 1 cut(s) | 529 |
| Hpy166II | GTNNAC | 4 cut(s) | 447, 668, 713, 730 |
| Hpy188I | TCNGA | 8 cut(s) | 25, 48, 153, 202, 325, 474, 562, 774 |
| Hpy188III | TCNNGA | 6 cut(s) | 209, 236, 272, 359, 614, 831 |
| Hpy8I | GTNNAC | 4 cut(s) | 447, 668, 713, 730 |
| Hpy99I | CGWCG | 4 cut(s) | 32, 35, 562, 774 |
| HpyAV | CCTTC | 3 cut(s) | 154, 472, 508 |
| HpyCH4III | ACNGT | 1 cut(s) | 902 |
| HpyCH4IV | ACGT | 2 cut(s) | 30, 743 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 170, 293 |
| HpyF3I | CTNAG | 3 cut(s) | 150, 156, 704 |
| HpySE526I | ACGT | 2 cut(s) | 30, 743 |
| Hsp92I | GRCGYC | 1 cut(s) | 30 |
| Hsp92II | CATG | 2 cut(s) | 247, 379 |
| Kzo9I | GATC | 1 cut(s) | 469 |
| LguI | GCTCTTC | 1 cut(s) | 693 |
| LmnI | GCTCC | 1 cut(s) | 481 |
| MaeI | CTAG | 3 cut(s) | 62, 83, 236 |
| MaeII | ACGT | 2 cut(s) | 30, 743 |
| MalI | GATC | 1 cut(s) | 471 |
| MbiI | CCGCTC | 1 cut(s) | 427 |
| MboI | GATC | 1 cut(s) | 469 |
| MboII | GAAGA | 8 cut(s) | 110, 122, 128, 139, 142, 629, 710, 869 |
| MflI | RGATCY | 1 cut(s) | 469 |
| MhlI | GDGCHC | 1 cut(s) | 705 |
| MluCI | AATT | 4 cut(s) | 266, 420, 591, 655 |
| MlyI | GAGTC | 2 cut(s) | 452, 848 |
| MmeI | TCCRAC | 2 cut(s) | 540, 752 |
| MnlI | CCTC | 8 cut(s) | 67, 134, 205, 216, 277, 656, 681, 712 |
| Mph1103I | ATGCAT | 1 cut(s) | 684 |
| MseI | TTAA | 2 cut(s) | 464, 873 |
| MspA1I | CMGCKG | 3 cut(s) | 170, 487, 586 |
| MspI | CCGG | 2 cut(s) | 10, 395 |
| MspR9I | CCNGG | 1 cut(s) | 71 |
| Mva1269I | GAATGC | 2 cut(s) | 303, 682 |
| MvaI | CCWGG | 1 cut(s) | 71 |
| MvnI | CGCG | 1 cut(s) | 832 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 170, 293 |
| NdeI | CATATG | 1 cut(s) | 366 |
| NdeII | GATC | 1 cut(s) | 469 |
| NlaIII | CATG | 2 cut(s) | 247, 379 |
| NruI | TCGCGA | 1 cut(s) | 832 |
| NsiI | ATGCAT | 1 cut(s) | 684 |
| NspI | RCATGY | 1 cut(s) | 247 |
| NspV | TTCGAA | 1 cut(s) | 307 |
| PciSI | GCTCTTC | 1 cut(s) | 693 |
| PctI | GAATGC | 2 cut(s) | 303, 682 |
| PfeI | GAWTC | 3 cut(s) | 146, 644, 827 |
| PflFI | GACNNNGTC | 1 cut(s) | 29 |
| PleI | GAGTC | 2 cut(s) | 451, 847 |
| PpsI | GAGTC | 2 cut(s) | 451, 847 |
| PshAI | GACNNNNGTC | 1 cut(s) | 838 |
| PshBI | ATTAAT | 1 cut(s) | 464 |
| Psp124BI | GAGCTC | 1 cut(s) | 705 |
| Psp6I | CCWGG | 1 cut(s) | 69 |
| PspFI | CCCAGC | 1 cut(s) | 487 |
| PspGI | CCWGG | 1 cut(s) | 69 |
| PstI | CTGCAG | 1 cut(s) | 826 |
| PsuI | RGATCY | 1 cut(s) | 469 |
| PsyI | GACNNNGTC | 1 cut(s) | 29 |
| PvuII | CAGCTG | 1 cut(s) | 487 |
| RruI | TCGCGA | 1 cut(s) | 832 |
| RsaI | GTAC | 4 cut(s) | 262, 385, 566, 746 |
| RsaNI | GTAC | 4 cut(s) | 261, 384, 565, 745 |
| SacI | GAGCTC | 1 cut(s) | 705 |
| SapI | GCTCTTC | 1 cut(s) | 693 |
| SaqAI | TTAA | 2 cut(s) | 464, 873 |
| Sau3AI | GATC | 1 cut(s) | 469 |
| SchI | GAGTC | 2 cut(s) | 452, 848 |
| ScrFI | CCNGG | 1 cut(s) | 71 |
| SduI | GDGCHC | 1 cut(s) | 705 |
| SfcI | CTRYAG | 2 cut(s) | 713, 822 |
| SfuI | TTCGAA | 1 cut(s) | 307 |
| Sse9I | AATT | 4 cut(s) | 266, 420, 591, 655 |
| SsiI | CCGC | 3 cut(s) | 170, 427, 586 |
| SspMI | CTAG | 3 cut(s) | 62, 83, 236 |
| SstI | GAGCTC | 1 cut(s) | 705 |
| StyD4I | CCNGG | 1 cut(s) | 69 |
| TaaI | ACNGT | 1 cut(s) | 902 |
| TaiI | ACGT | 2 cut(s) | 33, 746 |
| TaqI | TCGA | 2 cut(s) | 307, 686 |
| TasI | AATT | 4 cut(s) | 266, 420, 591, 655 |
| TatI | WGTACW | 3 cut(s) | 260, 383, 564 |
| TauI | GCSGC | 1 cut(s) | 173 |
| TfiI | GAWTC | 3 cut(s) | 146, 644, 827 |
| Tru1I | TTAA | 2 cut(s) | 464, 873 |
| Tru9I | TTAA | 2 cut(s) | 464, 873 |
| TscAI | CASTG | 1 cut(s) | 438 |
| TspDTI | ATGAA | 8 cut(s) | 111, 123, 129, 135, 291, 364, 535, 620 |
| TspRI | CASTG | 1 cut(s) | 438 |
| Tth111I | GACNNNGTC | 1 cut(s) | 29 |
| VspI | ATTAAT | 1 cut(s) | 464 |
| XapI | RAATTY | 1 cut(s) | 266 |
| XbaI | TCTAGA | 1 cut(s) | 235 |
| XceI | RCATGY | 1 cut(s) | 247 |
| XmiI | GTMKAC | 2 cut(s) | 667, 712 |
| XspI | CTAG | 3 cut(s) | 62, 83, 236 |
| ZraI | GACGTC | 1 cut(s) | 31 |
| Zsp2I | ATGCAT | 1 cut(s) | 684 |