RchiOBHm_Chr5g0072931

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000183
Physical Location & Seq
Reverse (-)
78977550 .. 78985955
8406 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34785

Sequence Viewer

Length: 2955 bp
ATGGGTCGCTGCACTTTCTCCTCGGACGATATTGTAATAAAGTCTCCGAATGATAAGAAACTGTACAGACTAATTAAGCTTGAGAATGGCCTCACTGCATTGCTCATTCATGACCCTGAGATATACCCACAAGGATCCAAGAGCCTTGAACCCAGTGAAGAAGATGAAGCAGCAGCTAAAGGTGGAGATGGTGCCTCAGAGACTAAGAAGGCAGCAGCGGCAATGTGCGTTGGAATGGGCAGCTTCTCTGACCCTATGGAAGCACAGGGGCTTGCGCACTTTCTTGAACATATGCTCTTCATGGGGAGTACAAAGTTTCCGGTTGAAAATGAGTATAACAGTTACTTGTCCAAGCATGGGGGCTCATCAAATGCTTACACAAAGGAAGAGCACACTTGCTACCATTTTGACGTGAAACGAGATTTTCTTAAAGGTGCCTTGAAAAGATTTTCTCAGTTTTTTGTTTCACCTCTAATGAGAAGTGAAGCCATGGAGCGAGAGGTACAGTCTATAGATTCAGAGTTTAACAGGGTTCTGCATAGCGATTTGTGGCGCCTTCAACAACTTCGAGACCATACATCCTCACCTGGTCACCCATTTAATAAATTCTCTTGGGGAAATAAGAAGAGCTTGGATGGTGCAAAGGAAAAAGGGATAAACTTGCAGGAACAGATATTAAAATTGTACAGAGACTATTACCATGGTGGATTAATGAAGCTAGTTGTTATTGGTGGAGAATCTCTCAATGTACTTGAGCAGTGGGTTCTGAAGTTGTTTGGAGACATCAGAAACGGTCCCCAAGTAAATCTTGAGTTCAAGGCAGAAGGTTCTATTTGGAACGTTGGAAAGCTTTACAGGCTAGAGGCTGTTAATGATGTTCCTATGCTCAACTTGGCATGGACGCTTCCATGTCTTACTCAACACTATTTGAAGAGCCCAGAATATTATTTATGGCATCTCCTTGCGCATGAGAGCAGGGGAGGTTTGCAGTTCTACCTCAAAACTAGAGGGTGGGCAGTATCTCTAAGTGCTTGGGTGGAGAGCTATTCTGTGGCTTATGTCTTTGGCATGAATATTTACCTCACCGACTCTGGATTGGAGAAGATTTTTGAAATTATTGGGTTTGTGTATCAATACATTAAACTATTGCGTCAAGTGTCACCACAAAAATGGATATTTCAAGAACTCCAGGATATTCAGAACATATGCTTTAGATTTGTAGAGGAGCAGCCCCAGGATGACTATGCTTCAGAACTTGCAGGAAATTTACTACTTTATCCTGCAGAGCATGTTATTTCTGCGCAATGTGTGTATGAGATTTGGGATGAGGAATTGATTGAATATGTTCTTAGTTTCTTCAGACCAGACAACATGAGGATGGATGTGATATCAAAGTTTTCAACTATGTCCAAAGATTTTCAGTGCGAGCCTTGGTTTGGTTCACATTATATTGAGGAAGATGTATCTCCGTTTTTGATGGATTTGTGGAAGGATCCTCCAGAGGTTAATGTTTCGTTGCATTTCCGAGAAAAGAATGAGTTTATTCCTTCTGATTTTTCCATTCGTTCTGATGGTCTTGATACTACATATACATCTTCTCCGAGATGTATACTTGATGAACCTTTGCTTAAGTTCTGGTACAAGCTTGATCGTACTTTTAAAGTTCCACGGGCAAATATATACTTTCGAATCAGTCTAAAGGGTGGATTTGATGACTTGAAGAGTTCTCTGTTGACTGTACTATATATCGACCTTCTTAGAGATGAGCTGAATGAGATTTTATATCAGGCCAGTTTTGCCCTGCTGGATACTTCTTTGTATGTGAATAATTGCTATCTGGAACTGAAGCTGTGGGGCTTCAATGATAAGCTTCCAGCTGTATTGTCAACAATTCTGACAACAACCAAAAAATTCCTGCCAACTTATGATCGTTTCAAGGTTATTAAAGAAAATATGGAGCGTCTGATAAAGAACACTAATATGAATCCTCTGAGTCATGCTGAATACTTGAAACTGCAAGTTTTGTACCAGAGATTCTACGATGTAGATGACCAGTTGCATGTTTTAAATGGATTGTCTGTTTCTGATGTGAAGTCATTTATTCCAGAGCTTTGGTCCCAGCTTTACATTGAAGGCCTTGGTCATGGAAATCTGTTAGAAGAAGAAGCAATTAGCCTTTTGAATATGGTGAAAACAATTTTTTCTGGACAACCACTTCCTACCGAACTGATGCTTAAGAACAAATGTATTTGTCTCACTCCTGATGCTAACCTCATTAGAGATGTCAGTGTGAAGTATAAGACTGAAACAAACTCTGTGATTCAGCTATGTTTTCAAATTGAGCGGGCAGTGGGGATTGACTCCACCAGATTGAGAGCATTAATAGATCTTTTTGATGAAATTATAGACGAACCACTTTACGATCAACTGAGGACGAAGGAGCAGCTGGGATATGTTGTTCAGTGTGACTGGGAGGTGATATTTGGTGTTTTTGGCTTTTATTTCATAGTCCAGTCGTCGGAGTACAACCCAATCTACTTGCAGCGGAGAGTTGACAACTTTATCAATGGTCTGGAAGACATTTTGCAAGGACTGGATGATGATTCCTTTGAGAATTACAGAGGTGGGCTAATGGCAAAGCTATTGGTGAAAAATTCATCCCTTATACATGAAACCAATAGATTTTGGAATCAGATTATTCATAAAAGGTATACATTTGACTATGCAAAGAGGGTGGCAGAAGAGCTCAGCAGTCTACAGAAGGAGGATGTTGTCAACTTTTACAAGACCTACTTGCAACAATCATCTCCCAAGCGTCGGAGACTTGGCATTCGTGTTTGGGGTTGCAACACTGACTTGAAAGAAGCTGAAGAATCGCGACCGGAGTCTGTGCAGGTCATTGAAGACCTTGAAGCCTTTAAGATGTCATCTGAGTTCTATCCTCACGGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

984

Amino Acids

114.05

Weight (kDa)

5.3

Isoelectric Point (pI)

45.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 67 - 196 1.7e-34 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 223 - 398 8.7e-19 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 404 - 687 1.4e-76 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 692 - 874 2.1e-15 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 797 - 896 2.5e-22 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 3 cut(s) 276, 966, 1302
Acc36I ACCTGC 1 cut(s) 2884
AccB1I GGYRCC 3 cut(s) 191, 434, 552
AccBSI CCGCTC 1 cut(s) 2344
AccI GTMKAC 3 cut(s) 1609, 2711, 2755
AccII CGCG 1 cut(s) 2878
AciI CCGC 3 cut(s) 218, 2344, 2545
AclI AACGTT 1 cut(s) 840
AclWI GGATC 4 cut(s) 129, 142, 1487, 1500
AcsI RAATTY 4 cut(s) 605, 1264, 1910, 2653
AcuI CTGAAG 5 cut(s) 788, 1233, 1342, 1865, 2889
AcyI GRCGYC 1 cut(s) 553
AfiI CCNNNNNNNGG 4 cut(s) 357, 1436, 2518, 2817
AflII CTTAAG 2 cut(s) 1628, 2234
AjiI CACGTC 1 cut(s) 412
AjnI CCWGG 3 cut(s) 586, 1188, 1233
AloI GAACNNNNNNTCC 2 cut(s) 2442, 2474
Alw21I GWGCWC 2 cut(s) 393, 2748
Alw26I GTCTC 7 cut(s) 48, 194, 564, 684, 774, 2258, 2815
AlwI GGATC 4 cut(s) 129, 142, 1487, 1500
AoxI GGCC 3 cut(s) 88, 1788, 2134
ApeKI GCWGC 9 cut(s) 9, 170, 173, 212, 215, 240, 1228, 2443, 2542
ApoI RAATTY 4 cut(s) 605, 1264, 1910, 2653
ArsI GACNNNNNNTTYG 2 cut(s) 2814, 2846
AseI ATTAAT 2 cut(s) 710, 2381
Asp700I GAANNNNTTC 1 cut(s) 1344
AspLEI GCGC 4 cut(s) 277, 555, 967, 1303
AspS9I GGNCC 2 cut(s) 794, 2115
AsuHPI GGTGA 8 cut(s) 459, 576, 584, 1075, 1152, 2200, 2488, 2659
AsuII TTCGAA 1 cut(s) 1687
AvaII GGWCC 2 cut(s) 794, 2115
BamHI GGATCC 2 cut(s) 134, 1492
BanI GGYRCC 3 cut(s) 191, 434, 552
BanII GRGCYC 3 cut(s) 365, 938, 2748
BbsI GAAGAC 2 cut(s) 2583, 2910
Bbv12I GWGCWC 2 cut(s) 393, 2748
BbvI GCAGC 8 cut(s) 182, 185, 224, 227, 252, 1240, 2455, 2554
BccI CCATC 5 cut(s) 182, 629, 1372, 1471, 1565
BciT130I CCWGG 3 cut(s) 588, 1190, 1235
BciVI GTATCC 1 cut(s) 1801
BcoDI GTCTC 7 cut(s) 48, 194, 564, 684, 774, 2258, 2815
BfaI CTAG 3 cut(s) 719, 860, 1005
BfmI CTRYAG 3 cut(s) 510, 1281, 2756
BfoI RGCGCY 1 cut(s) 556
BfrI CTTAAG 2 cut(s) 1628, 2234
BfuAI ACCTGC 1 cut(s) 2884
BfuI GTATCC 1 cut(s) 1801
BglII AGATCT 1 cut(s) 2386
BlpI GCTNAGC 1 cut(s) 2747
Bme1390I CCNGG 3 cut(s) 588, 1190, 1235
Bme18I GGWCC 2 cut(s) 794, 2115
BmgBI CACGTC 1 cut(s) 412
BmgT120I GGNCC 2 cut(s) 794, 2115
BmiI GGNNCC 7 cut(s) 136, 193, 436, 554, 796, 1494, 2117
BmrFI CCNGG 3 cut(s) 588, 1190, 1235
BmrI ACTGGG 2 cut(s) 147, 2479
BmsI GCATC 3 cut(s) 964, 2220, 2254
BmuI ACTGGG 2 cut(s) 147, 2479
BoxI GACNNNNGTC 1 cut(s) 2884
BpiI GAAGAC 2 cut(s) 2583, 2910
BplI GAGNNNNNCTC 2 cut(s) 726, 758
BpmI CTGGAG 2 cut(s) 1172, 1482
Bpu1102I GCTNAGC 1 cut(s) 2747
Bpu14I TTCGAA 1 cut(s) 1687
BpuEI CTTGAG 3 cut(s) 101, 773, 830
BsaHI GRCGYC 1 cut(s) 553
BsaI GGTCTC 1 cut(s) 564
BsaJI CCNNGG 7 cut(s) 21, 489, 700, 1233, 1430, 1667, 2137
BsaWI WCCGGW 2 cut(s) 319, 2881
BsaXI ACNNNNNCTCC 2 cut(s) 1582, 1612
Bsc4I CCNNNNNNNGG 4 cut(s) 357, 1436, 2518, 2817
Bse1I ACTGG 6 cut(s) 153, 1791, 2053, 2474, 2512, 2598
Bse3DI GCAATG 3 cut(s) 98, 228, 1310
BseBI CCWGG 3 cut(s) 588, 1190, 1235
BseDI CCNNGG 7 cut(s) 21, 489, 700, 1233, 1430, 1667, 2137
BseGI GGATG 9 cut(s) 578, 640, 1243, 1330, 1383, 1387, 2602, 2657, 2773
BseLI CCNNNNNNNGG 4 cut(s) 357, 1436, 2518, 2817
BseMI GCAATG 3 cut(s) 98, 228, 1310
BseMII CTCAG 7 cut(s) 108, 210, 467, 1982, 2420, 2761, 2922
BseNI ACTGG 6 cut(s) 153, 1791, 2053, 2474, 2512, 2598
BseRI GAGGAG 2 cut(s) 10, 1238
BseXI GCAGC 8 cut(s) 182, 185, 224, 227, 252, 1240, 2455, 2554
BseYI CCCAGC 2 cut(s) 2118, 2446
BsgI GTGCAG 1 cut(s) 2912
Bsh1236I CGCG 1 cut(s) 2878
Bsh1285I CGRYCG 1 cut(s) 2882
BshFI GGCC 3 cut(s) 90, 1790, 2136
BshNI GGYRCC 3 cut(s) 191, 434, 552
BsiEI CGRYCG 1 cut(s) 2882
BsiHKAI GWGCWC 2 cut(s) 393, 2748
BsiSI CCGG 2 cut(s) 320, 2882
BslFI GGGAC 2 cut(s) 780, 2101
BslI CCNNNNNNNGG 4 cut(s) 357, 1436, 2518, 2817
BsmAI GTCTC 7 cut(s) 48, 194, 564, 684, 774, 2258, 2815
BsmFI GGGAC 2 cut(s) 780, 2101
BsmI GAATGC 1 cut(s) 2829
BsnI GGCC 3 cut(s) 90, 1790, 2136
Bso31I GGTCTC 1 cut(s) 564
Bsp119I TTCGAA 1 cut(s) 1687
Bsp1286I GDGCHC 4 cut(s) 365, 393, 938, 2748
Bsp1407I TGTACA 2 cut(s) 63, 684
Bsp143I GATC 6 cut(s) 134, 1492, 1648, 1927, 2386, 2422
Bsp1720I GCTNAGC 1 cut(s) 2747
Bsp19I CCATGG 2 cut(s) 489, 700
Bsp68I TCGCGA 1 cut(s) 2878
BspACI CCGC 3 cut(s) 218, 2344, 2545
BspANI GGCC 3 cut(s) 90, 1790, 2136
BspCNI CTCAG 7 cut(s) 109, 209, 466, 1983, 2421, 2760, 2923
BspFNI CGCG 1 cut(s) 2878
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 7 cut(s) 136, 193, 436, 554, 796, 1494, 2117
BspMAI CTGCAG 1 cut(s) 1285
BspMI ACCTGC 1 cut(s) 2884
BspPI GGATC 4 cut(s) 129, 142, 1487, 1500
BspQI GCTCTTC 5 cut(s) 302, 381, 620, 926, 2736
BspT104I TTCGAA 1 cut(s) 1687
BspT107I GGYRCC 3 cut(s) 191, 434, 552
BspTI CTTAAG 2 cut(s) 1628, 2234
BspTNI GGTCTC 1 cut(s) 564
BsrBI CCGCTC 1 cut(s) 2344
BsrDI GCAATG 3 cut(s) 98, 228, 1310
BsrGI TGTACA 2 cut(s) 63, 684
BsrI ACTGG 6 cut(s) 153, 1791, 2053, 2474, 2512, 2598
BssECI CCNNGG 7 cut(s) 21, 489, 700, 1233, 1430, 1667, 2137
BssMI GATC 6 cut(s) 134, 1492, 1648, 1927, 2386, 2422
BssNAI GTATAC 2 cut(s) 1610, 2712
BssNI GRCGYC 1 cut(s) 553
BssT1I CCWWGG 4 cut(s) 489, 700, 1430, 2137
Bst1107I GTATAC 2 cut(s) 1610, 2712
Bst2UI CCWGG 3 cut(s) 588, 1190, 1235
Bst4CI ACNGT 6 cut(s) 63, 341, 507, 794, 1738, 2948
Bst6I CTCTTC 6 cut(s) 302, 381, 620, 926, 1715, 2736
BstACI GRCGYC 1 cut(s) 553
BstAFI CTTAAG 2 cut(s) 1628, 2234
BstAUI TGTACA 2 cut(s) 63, 684
BstBI TTCGAA 1 cut(s) 1687
BstC8I GCNNGC 3 cut(s) 273, 1427, 2346
BstDSI CCRYGG 3 cut(s) 489, 700, 1667
BstEII GGTNACC 1 cut(s) 590
BstF5I GGATG 9 cut(s) 578, 640, 1243, 1330, 1383, 1387, 2602, 2657, 2773
BstFNI CGCG 1 cut(s) 2878
BstH2I RGCGCY 1 cut(s) 556
BstHHI GCGC 4 cut(s) 277, 555, 967, 1303
BstKTI GATC 6 cut(s) 137, 1495, 1651, 1930, 2389, 2425
BstMAI GTCTC 7 cut(s) 48, 194, 564, 684, 774, 2258, 2815
BstMBI GATC 6 cut(s) 134, 1492, 1648, 1927, 2386, 2422
BstMCI CGRYCG 1 cut(s) 2882
BstMWI GCNNNNNNNGC 3 cut(s) 218, 856, 1796
BstNI CCWGG 3 cut(s) 588, 1190, 1235
BstNSI RCATGY 2 cut(s) 1292, 2063
BstPAI GACNNNNGTC 1 cut(s) 2884
BstPI GGTNACC 1 cut(s) 590
BstSCI CCNGG 3 cut(s) 586, 1188, 1233
BstSFI CTRYAG 3 cut(s) 510, 1281, 2756
BstUI CGCG 1 cut(s) 2878
BstV1I GCAGC 8 cut(s) 182, 185, 224, 227, 252, 1240, 2455, 2554
BstV2I GAAGAC 2 cut(s) 2583, 2910
BstX2I RGATCY 3 cut(s) 134, 1492, 2386
BstXI CCANNNNNNTGG 2 cut(s) 1170, 2112
BstYI RGATCY 3 cut(s) 134, 1492, 2386
BstZ17I GTATAC 2 cut(s) 1610, 2712
BsuI GTATCC 1 cut(s) 1801
BsuRI GGCC 3 cut(s) 90, 1790, 2136
BtgI CCRYGG 3 cut(s) 489, 700, 1667
BtrI CACGTC 1 cut(s) 412
BtsCI GGATG 9 cut(s) 578, 640, 1243, 1330, 1383, 1387, 2602, 2657, 2773
BtsI GCAGTG 3 cut(s) 93, 764, 2355
BtsIMutI CAGTG 8 cut(s) 93, 160, 764, 1427, 2293, 2355, 2468, 2850
BtuMI TCGCGA 1 cut(s) 2878
BveI ACCTGC 1 cut(s) 2884
Cac8I GCNNGC 3 cut(s) 273, 1427, 2346
CciI TCATGA 1 cut(s) 109
CfoI GCGC 4 cut(s) 277, 555, 967, 1303
Cfr13I GGNCC 2 cut(s) 794, 2115
CseI GACGC 4 cut(s) 910, 1139, 1949, 2804
CsiI ACCWGGT 1 cut(s) 586
CspCI CAANNNNNGTGG 2 cut(s) 2715, 2750
DinI GGCGCC 1 cut(s) 554
DpnI GATC 6 cut(s) 136, 1494, 1650, 1929, 2388, 2424
DpnII GATC 6 cut(s) 134, 1492, 1648, 1927, 2386, 2422
DraI TTTAAA 2 cut(s) 1660, 2067
Eam1104I CTCTTC 6 cut(s) 302, 381, 620, 926, 1715, 2736
EarI CTCTTC 6 cut(s) 302, 381, 620, 926, 1715, 2736
Ecl136II GAGCTC 1 cut(s) 2746
Eco130I CCWWGG 4 cut(s) 489, 700, 1430, 2137
Eco147I AGGCCT 1 cut(s) 2136
Eco24I GRGCYC 3 cut(s) 365, 938, 2748
Eco31I GGTCTC 1 cut(s) 564
Eco32I GATATC 1 cut(s) 1389
Eco47I GGWCC 2 cut(s) 794, 2115
Eco53kI GAGCTC 1 cut(s) 2746
Eco57I CTGAAG 5 cut(s) 788, 1233, 1342, 1865, 2889
Eco91I GGTNACC 1 cut(s) 590
EcoICRI GAGCTC 1 cut(s) 2746
EcoO65I GGTNACC 1 cut(s) 590
EcoRII CCWGG 3 cut(s) 586, 1188, 1233
EcoRV GATATC 1 cut(s) 1389
EcoT14I CCWWGG 4 cut(s) 489, 700, 1430, 2137
EcoT38I GRGCYC 3 cut(s) 365, 938, 2748
EgeI GGCGCC 1 cut(s) 554
EheI GGCGCC 1 cut(s) 554
ErhI CCWWGG 4 cut(s) 489, 700, 1430, 2137
FalI AAGNNNNNCTT 6 cut(s) 614, 646, 792, 824, 2777, 2809
FaqI GGGAC 2 cut(s) 780, 2101
FauI CCCGC 1 cut(s) 2337
FauNDI CATATG 2 cut(s) 291, 1205
FblI GTMKAC 3 cut(s) 1609, 2711, 2755
FokI GGATG 9 cut(s) 565, 647, 1250, 1337, 1390, 1394, 2609, 2644, 2780
FriOI GRGCYC 3 cut(s) 365, 938, 2748
FspBI CTAG 3 cut(s) 719, 860, 1005
FspI TGCGCA 3 cut(s) 276, 966, 1302
GlaI GCGC 4 cut(s) 276, 554, 966, 1302
GsaI CCCAGC 2 cut(s) 2122, 2450
GsuI CTGGAG 2 cut(s) 1172, 1482
HaeII RGCGCY 1 cut(s) 556
HaeIII GGCC 3 cut(s) 90, 1790, 2136
HapII CCGG 2 cut(s) 320, 2882
HgaI GACGC 4 cut(s) 910, 1139, 1949, 2804
HhaI GCGC 4 cut(s) 277, 555, 967, 1303
Hin1I GRCGYC 1 cut(s) 553
Hin6I GCGC 4 cut(s) 275, 553, 965, 1301
HinP1I GCGC 4 cut(s) 275, 553, 965, 1301
HincII GTYRAC 4 cut(s) 1734, 1887, 2554, 2776
HindII GTYRAC 4 cut(s) 1734, 1887, 2554, 2776
HindIII AAGCTT 4 cut(s) 77, 848, 1643, 1868
HpaII CCGG 2 cut(s) 320, 2882
HphI GGTGA 8 cut(s) 459, 576, 584, 1075, 1152, 2200, 2488, 2659
Hpy166II GTNNAC 8 cut(s) 1442, 1610, 1734, 1887, 2554, 2712, 2756, 2776
Hpy8I GTNNAC 8 cut(s) 1442, 1610, 1734, 1887, 2554, 2712, 2756, 2776
Hpy99I CGWCG 2 cut(s) 2521, 2820
HpyAV CCTTC 9 cut(s) 202, 566, 818, 1483, 1557, 1763, 2126, 2431, 2755
HpyCH4III ACNGT 6 cut(s) 63, 341, 507, 794, 1738, 2948
HpyCH4IV ACGT 2 cut(s) 411, 840
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 856, 1796
HpySE526I ACGT 2 cut(s) 411, 840
Hsp92I GRCGYC 1 cut(s) 553
HspAI GCGC 4 cut(s) 275, 553, 965, 1301
KasI GGCGCC 1 cut(s) 552
Kzo9I GATC 6 cut(s) 134, 1492, 1648, 1927, 2386, 2422
LguI GCTCTTC 5 cut(s) 302, 381, 620, 926, 2736
LmnI GCTCC 4 cut(s) 493, 1225, 1957, 2440
Lsp1109I GCAGC 8 cut(s) 182, 185, 224, 227, 252, 1240, 2455, 2554
LweI GCATC 3 cut(s) 964, 2220, 2254
MabI ACCWGGT 1 cut(s) 586
MaeI CTAG 3 cut(s) 719, 860, 1005
MaeII ACGT 2 cut(s) 411, 840
MaeIII GTNAC 4 cut(s) 341, 590, 1158, 2465
MalI GATC 6 cut(s) 136, 1494, 1650, 1929, 2388, 2424
MbiI CCGCTC 1 cut(s) 2344
MboI GATC 6 cut(s) 134, 1492, 1648, 1927, 2386, 2422
MflI RGATCY 3 cut(s) 134, 1492, 2386
MhlI GDGCHC 4 cut(s) 365, 393, 938, 2748
Mly113I GGCGCC 1 cut(s) 553
MlyI GAGTC 4 cut(s) 1082, 2002, 2354, 2894
MmeI TCCRAC 4 cut(s) 211, 823, 2499, 2798
MroXI GAANNNNTTC 1 cut(s) 1344
MslI CAYNNNNRTG 3 cut(s) 1168, 1376, 1979
MspA1I CMGCKG 4 cut(s) 218, 1877, 2446, 2545
MspCI CTTAAG 2 cut(s) 1628, 2234
MspI CCGG 2 cut(s) 320, 2882
MspR9I CCNGG 3 cut(s) 588, 1190, 1235
Mva1269I GAATGC 1 cut(s) 2829
MvaI CCWGG 3 cut(s) 588, 1190, 1235
MvnI CGCG 1 cut(s) 2878
MwoI GCNNNNNNNGC 3 cut(s) 218, 856, 1796
NarI GGCGCC 1 cut(s) 553
NcoI CCATGG 2 cut(s) 489, 700
NdeI CATATG 2 cut(s) 291, 1205
NdeII GATC 6 cut(s) 134, 1492, 1648, 1927, 2386, 2422
NlaIV GGNNCC 7 cut(s) 136, 193, 436, 554, 796, 1494, 2117
NmuCI GTSAC 3 cut(s) 590, 1158, 2465
NruI TCGCGA 1 cut(s) 2878
NsbI TGCGCA 3 cut(s) 276, 966, 1302
NspI RCATGY 2 cut(s) 1292, 2063
NspV TTCGAA 1 cut(s) 1687
PagI TCATGA 1 cut(s) 109
PceI AGGCCT 1 cut(s) 2136
PciSI GCTCTTC 5 cut(s) 302, 381, 620, 926, 2736
PctI GAATGC 1 cut(s) 2829
PdmI GAANNNNTTC 1 cut(s) 1344
PfeI GAWTC 9 cut(s) 515, 737, 1689, 1984, 2034, 2320, 2603, 2689, 2873
PfoI TCCNGGA 1 cut(s) 1188
PleI GAGTC 4 cut(s) 1082, 2001, 2354, 2893
PluTI GGCGCC 1 cut(s) 556
PpsI GAGTC 4 cut(s) 1082, 2001, 2354, 2893
PshAI GACNNNNGTC 1 cut(s) 2884
PshBI ATTAAT 2 cut(s) 710, 2381
Psp124BI GAGCTC 1 cut(s) 2748
Psp1406I AACGTT 1 cut(s) 840
Psp6I CCWGG 3 cut(s) 586, 1188, 1233
PspEI GGTNACC 1 cut(s) 590
PspFI CCCAGC 2 cut(s) 2118, 2446
PspGI CCWGG 3 cut(s) 586, 1188, 1233
PspN4I GGNNCC 7 cut(s) 136, 193, 436, 554, 796, 1494, 2117
PspPI GGNCC 2 cut(s) 794, 2115
PstI CTGCAG 1 cut(s) 1285
PsuI RGATCY 3 cut(s) 134, 1492, 2386
PvuII CAGCTG 2 cut(s) 1877, 2446
RruI TCGCGA 1 cut(s) 2878
RseI CAYNNNNRTG 3 cut(s) 1168, 1376, 1979
SacI GAGCTC 1 cut(s) 2748
SapI GCTCTTC 5 cut(s) 302, 381, 620, 926, 2736
Sau3AI GATC 6 cut(s) 134, 1492, 1648, 1927, 2386, 2422
Sau96I GGNCC 2 cut(s) 794, 2115
SchI GAGTC 4 cut(s) 1082, 2002, 2354, 2894
ScrFI CCNGG 3 cut(s) 588, 1190, 1235
SduI GDGCHC 4 cut(s) 365, 393, 938, 2748
SexAI ACCWGGT 1 cut(s) 586
SfaNI GCATC 3 cut(s) 964, 2220, 2254
SfcI CTRYAG 3 cut(s) 510, 1281, 2756
SfoI GGCGCC 1 cut(s) 554
SfuI TTCGAA 1 cut(s) 1687
SinI GGWCC 2 cut(s) 794, 2115
SmiMI CAYNNNNRTG 3 cut(s) 1168, 1376, 1979
SmlI CTYRAG 5 cut(s) 80, 752, 809, 1628, 2234
SmoI CTYRAG 5 cut(s) 80, 752, 809, 1628, 2234
SseBI AGGCCT 1 cut(s) 2136
SsiI CCGC 3 cut(s) 218, 2344, 2545
SspDI GGCGCC 1 cut(s) 552
SspI AATATT 2 cut(s) 944, 1075
SspMI CTAG 3 cut(s) 719, 860, 1005
SstI GAGCTC 1 cut(s) 2748
StuI AGGCCT 1 cut(s) 2136
StyD4I CCNGG 3 cut(s) 586, 1188, 1233
StyI CCWWGG 4 cut(s) 489, 700, 1430, 2137
TaaI ACNGT 6 cut(s) 63, 341, 507, 794, 1738, 2948
TaiI ACGT 2 cut(s) 414, 843
TaqI TCGA 3 cut(s) 568, 1687, 1749
TatI WGTACW 6 cut(s) 63, 308, 684, 748, 1738, 2523
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 9 cut(s) 515, 737, 1689, 1984, 2034, 2320, 2603, 2689, 2873
TscAI CASTG 8 cut(s) 100, 160, 764, 1427, 2293, 2355, 2468, 2857
TseFI GTSAC 3 cut(s) 590, 1158, 2465
TseI GCWGC 9 cut(s) 9, 170, 173, 212, 215, 240, 1228, 2443, 2542
Tsp45I GTSAC 3 cut(s) 590, 1158, 2465
TspGWI ACGGA 1 cut(s) 1458
TspRI CASTG 8 cut(s) 100, 160, 764, 1427, 2293, 2355, 2468, 2857
Vha464I CTTAAG 2 cut(s) 1628, 2234
VpaK11BI GGWCC 2 cut(s) 794, 2115
VspI ATTAAT 2 cut(s) 710, 2381
XapI RAATTY 4 cut(s) 605, 1264, 1910, 2653
XceI RCATGY 2 cut(s) 1292, 2063
XmiI GTMKAC 3 cut(s) 1609, 2711, 2755
XmnI GAANNNNTTC 1 cut(s) 1344
XspI CTAG 3 cut(s) 719, 860, 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.